tnika gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005163391.4	XP_005163448.1	TRAF2 and NCK interacting kinase a isoform X5
XM_005163392.4	XP_005163449.1	TRAF2 and NCK interacting kinase a isoform X6
XM_005163387.3	XP_005163444.1	TRAF2 and NCK interacting kinase a isoform X1
XM_005163389.3	XP_005163446.1	TRAF2 and NCK interacting kinase a isoform X3
NM_199703.1	NP_955997.1	TRAF2 and NCK interacting kinase a
XM_005163392.3	XP_005163449.1	TRAF2 and NCK interacting kinase a isoform X6
XM_009298543.2	XP_009296818.1	TRAF2 and NCK interacting kinase a isoform X8
XM_005163389.4	XP_005163446.1	TRAF2 and NCK interacting kinase a isoform X3
XM_005163388.3	XP_005163445.1	TRAF2 and NCK interacting kinase a isoform X2
XM_005163388.4	XP_005163445.1	TRAF2 and NCK interacting kinase a isoform X2
XM_005163393.4	XP_005163450.1	TRAF2 and NCK interacting kinase a isoform X7
XM_005163391.3	XP_005163448.1	TRAF2 and NCK interacting kinase a isoform X5
XM_005163390.4	XP_005163447.1	TRAF2 and NCK interacting kinase a isoform X4
XM_005163390.3	XP_005163447.1	TRAF2 and NCK interacting kinase a isoform X4
XM_005163393.3	XP_005163450.1	TRAF2 and NCK interacting kinase a isoform X7
XM_005163387.4	XP_005163444.1	TRAF2 and NCK interacting kinase a isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa07038	XM_005163393.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa39841	XM_009298543.2 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa31229	NM_199703.1 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07035	XM_005163390.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07036	XM_005163391.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07037	XM_005163392.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07032	XM_005163387.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07033	XM_005163388.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07034	XM_005163389.4 Latest version!	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa07037	XM_005163392.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07034	XM_005163389.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07033	XM_005163388.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07036	XM_005163391.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07035	XM_005163390.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07038	XM_005163393.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07032	XM_005163387.3	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

tnika ( XM_005163393.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163450.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa07038
Clone ID Related Accession (Same CDS sequence)	XM_005163393.4 , XM_005163393.3
Accession Version	XM_005163393.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163393.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATAA ATCATTCAGT TAGCCATATA ACTCACGTTA TCTATTTGTT CATGCATATG 
TTAAGCCACT CGCTTTTCCA TCTTATAAGT GACATCATAA AAGCCCCTTT TTCATGTTTA 
ACCCTGGTTC TGATTCTGAG CCCCATCAGA TGGCTACTGT CCCACTTGGA AGACCTCTTT 
TTTTGTGTTG AGGAGCGATC TAAACTGAAC CGCCAGAGCT CTCCTGCCCT ACAGCATAAA 
GTGGCCAATC GTATCTCTGA CCCTTCCCTC CCGCCTCGCT CTGAGTCCTT CAGCAGTGGA 
GGAATGCAGC CAGCACGCAC CCCTCCTATG CACCGCCCTG TGGAGCCCCA GATGGCTCAT 
TTGGTTCCAG TGAGGTCCCA TAGCTCCTCT ATGTCAGTCT CGCAGTCTTT GCATGATCAA 
TCTGCAAAGG GTGTTTCAGC CTTCCAGGAA AGCCTGGTGT CCAATCGACC AGAGATGCCT 
CGACAGAACT CCGACCCGAC TTCAGAGATG CCCCCTCCTG CCCCTCGCAT GGCCAGCCAT 
GAGGAAAAAC CTGACCGCAG TTCCCCCTGG CTGAGGGAAG ACGCCGTTCC TCCCAAGTTG 
TCCGGTACTA CCCAGGTGCC CCAGAGGACC ACTTCTATCT CCCCTGCTCT TGCTAGGAAA 
AACTCTCCTG GAAACGGAGA GTCCAGTGGA GGGGGGCGGT CGGCCTCTCA GCTCATTCGC 
ACCAGTAATC CAGACCTGAG GAGGACAGAG TTGTCTCTGG ATGTGGCCCT GCAGAGAACC 
AGCAGCAACA GCTCCTCCAG CTCCAGCACA CCCAGCTCTC AGGGTGGATC CCAGCCTGGA 
TCCAGTGAGA GGAACCAGCC AAGAGGATCT GGTAAATCAG AGGGATCTCC AGTGGCATTA 
CAGGAAACCT CCAAGCCCAT TCAGGAGGAA GGCAGAGAGC TAAGCAAACC CAGTCGGCCT 
GCAGATCTGA CTGCCCTGGC TAAAGAACTG AGGGAGCTGC GAATAGATGA GGTCAGTCGA 
CCCCCTCTTA AACTCACAGA CTACTCCTCT TCTAGTGAGG ACTCTGATAG CAGTGAGGAT 
GAGGATGGTA CGGTGGGACA CGACGGCACT GTGTCAGTCA GTGACATACC CAGGATCATG 
CCATCCGTTC AAGGAAATAA TGAGTCGTTC ACGAGCAGCC ATGATGATTC CCTCACTGAC 
TCCTACAATG ATGAGTCTAA AGATGGGACA CTGAGGATGC GAGAGAACAA TGAGAGGCAA 
CGCTTAGGCC ATGGCGAGAG CAATGGCTTT GCAGGCCATG GAAACCTACC AGACCTCCTG 
CAGCAGAGCC ACTCTCCATC CGGAACTCCA ACAGGCCTCA ATTCCCAAGA CCTGGACTCT 
ATGGATGAGT TTGGTCTTGG CGGTGGTTCG AAAGCTTCTT TCACTCCTTT TGTGGACCCA 
CGAGTGTATC AAACCTCCCC CACAGACGAC AACGATGAGA GCTCAGCAGC CGCTATGTTT 
GCGAATGAGC TGTTGCGTCA AGAGCAGGCC AAACTGAATG AGGCCCGCAA GATCTCTGTG 
GTGAACGTCA ACCCCACCAA CATCAGACCT CACAGCGACA CGCCAGAGAT CCGCAAATAC 
AAAAAACGCT TTAACTCCGA GATCTTGTGT GCTGCTCTAT GGGGTGTTAA CCTCTTGGTG 
GGCACTGAAA ATGGTCTGAT GCTGCTGGAT CGTAGCGGTC AGGGCAAGGT TTATAACCTC 
ATCACCCGCA GACGCTTCCA ACAGATGGAT GTGCTGGAGG GTCTAAATGT CCTGGTCACC 
ATATCAGGGA AAAAAAATAA GCTCCGTGTG TACTACTTGT CATGGCTAAG AAACCGAATA 
CTGCACAATG ACCCGGAGGT GGAGAAGAAG CAGGGCTGGA TCACTGTTGG TGAACTCGAA 
GGCTGCATCC ACTATAAAGT TGTGAAATAC GAGAGAATCA AATTCCTGGT GATTGCACTG 
AAGAACTCGG TAGAGATTTA CGCATGGGCC CCCAAGCCGT ACCATAAATT CATGGCTTTC 
AAGTCGTTCA CAGAGCTGCA GCACAGACCT CAGCTGGTGG ACCTCACAGT AGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA CGGCTCTAGT GTGGGCTTTC ACGTCATCGA TGTGGATTCC 
GGAAGCCCCT ACGACATCTA CATACCGTCA CATATTCAGA GCACTGTGAC TCCTCATGCC 
ATCGTGGTGC TTCCGAAGAC TGATGGCATG GAGATGCTGC TGTGTTATGA AGATGAGGGC 
GTTTATGTCA ATACCTACGG CAGAATCACC AAGGACGTTG TCTTGCAGTG GGGAGAGATG 
CCCACCTCTG TTGCTTATAT TCATTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CTGTGGAAAC TGGTCACCTG GATGGGGTTT TCATGCACAA GAGAGCTCAG 
AGACTCAAGT TCTTGTGTGA GAGGAACGAC AAGGTGTTCT TCGCCTCAGT GCGGTCAGGT 
GGCAGCAGTC AAGTGTTCTT CATGACCCTG AATAGGAACT CTCTGATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163450.1
CDS	121..2697
Translation	MYINHSVSHITHVIYLFMHMLSHSLFHLISDIIKAPFSCLTLVLILSPIRWLLSHLEDLFFCVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163393.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163393.4

ORF Insert Sequence:

ATGTACATAA ATCATTCAGT TAGCCATATA ACTCACGTTA TCTATTTGTT CATGCATATG 
TTAAGCCACT CGCTTTTCCA TCTTATAAGT GACATCATAA AAGCCCCTTT TTCATGTTTA 
ACCCTGGTTC TGATTCTGAG CCCCATCAGA TGGCTACTGT CCCACTTGGA AGACCTCTTT 
TTTTGTGTTG AGGAGCGATC TAAACTGAAC CGCCAGAGCT CTCCTGCCCT ACAGCATAAA 
GTGGCCAATC GTATCTCTGA CCCTTCCCTC CCGCCTCGCT CTGAGTCCTT CAGCAGTGGA 
GGAATGCAGC CAGCACGCAC CCCTCCTATG CACCGCCCTG TGGAGCCCCA GATGGCTCAT 
TTGGTTCCAG TGAGGTCCCA TAGCTCCTCT ATGTCAGTCT CGCAGTCTTT GCATGATCAA 
TCTGCAAAGG GTGTTTCAGC CTTCCAGGAA AGCCTGGTGT CCAATCGACC AGAGATGCCT 
CGACAGAACT CCGACCCGAC TTCAGAGATG CCCCCTCCTG CCCCTCGCAT GGCCAGCCAT 
GAGGAAAAAC CTGACCGCAG TTCCCCCTGG CTGAGGGAAG ACGCCGTTCC TCCCAAGTTG 
TCCGGTACTA CCCAGGTGCC CCAGAGGACC ACTTCTATCT CCCCTGCTCT TGCTAGGAAA 
AACTCTCCTG GAAACGGAGA GTCCAGTGGA GGGGGGCGGT CGGCCTCTCA GCTCATTCGC 
ACCAGTAATC CAGACCTGAG GAGGACAGAG TTGTCTCTGG ATGTGGCCCT GCAGAGAACC 
AGCAGCAACA GCTCCTCCAG CTCCAGCACA CCCAGCTCTC AGGGTGGATC CCAGCCTGGA 
TCCAGTGAGA GGAACCAGCC AAGAGGATCT GGTAAATCAG AGGGATCTCC AGTGGCATTA 
CAGGAAACCT CCAAGCCCAT TCAGGAGGAA GGCAGAGAGC TAAGCAAACC CAGTCGGCCT 
GCAGATCTGA CTGCCCTGGC TAAAGAACTG AGGGAGCTGC GAATAGATGA GGTCAGTCGA 
CCCCCTCTTA AACTCACAGA CTACTCCTCT TCTAGTGAGG ACTCTGATAG CAGTGAGGAT 
GAGGATGGTA CGGTGGGACA CGACGGCACT GTGTCAGTCA GTGACATACC CAGGATCATG 
CCATCCGTTC AAGGAAATAA TGAGTCGTTC ACGAGCAGCC ATGATGATTC CCTCACTGAC 
TCCTACAATG ATGAGTCTAA AGATGGGACA CTGAGGATGC GAGAGAACAA TGAGAGGCAA 
CGCTTAGGCC ATGGCGAGAG CAATGGCTTT GCAGGCCATG GAAACCTACC AGACCTCCTG 
CAGCAGAGCC ACTCTCCATC CGGAACTCCA ACAGGCCTCA ATTCCCAAGA CCTGGACTCT 
ATGGATGAGT TTGGTCTTGG CGGTGGTTCG AAAGCTTCTT TCACTCCTTT TGTGGACCCA 
CGAGTGTATC AAACCTCCCC CACAGACGAC AACGATGAGA GCTCAGCAGC CGCTATGTTT 
GCGAATGAGC TGTTGCGTCA AGAGCAGGCC AAACTGAATG AGGCCCGCAA GATCTCTGTG 
GTGAACGTCA ACCCCACCAA CATCAGACCT CACAGCGACA CGCCAGAGAT CCGCAAATAC 
AAAAAACGCT TTAACTCCGA GATCTTGTGT GCTGCTCTAT GGGGTGTTAA CCTCTTGGTG 
GGCACTGAAA ATGGTCTGAT GCTGCTGGAT CGTAGCGGTC AGGGCAAGGT TTATAACCTC 
ATCACCCGCA GACGCTTCCA ACAGATGGAT GTGCTGGAGG GTCTAAATGT CCTGGTCACC 
ATATCAGGGA AAAAAAATAA GCTCCGTGTG TACTACTTGT CATGGCTAAG AAACCGAATA 
CTGCACAATG ACCCGGAGGT GGAGAAGAAG CAGGGCTGGA TCACTGTTGG TGAACTCGAA 
GGCTGCATCC ACTATAAAGT TGTGAAATAC GAGAGAATCA AATTCCTGGT GATTGCACTG 
AAGAACTCGG TAGAGATTTA CGCATGGGCC CCCAAGCCGT ACCATAAATT CATGGCTTTC 
AAGTCGTTCA CAGAGCTGCA GCACAGACCT CAGCTGGTGG ACCTCACAGT AGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA CGGCTCTAGT GTGGGCTTTC ACGTCATCGA TGTGGATTCC 
GGAAGCCCCT ACGACATCTA CATACCGTCA CATATTCAGA GCACTGTGAC TCCTCATGCC 
ATCGTGGTGC TTCCGAAGAC TGATGGCATG GAGATGCTGC TGTGTTATGA AGATGAGGGC 
GTTTATGTCA ATACCTACGG CAGAATCACC AAGGACGTTG TCTTGCAGTG GGGAGAGATG 
CCCACCTCTG TTGCTTATAT TCATTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CTGTGGAAAC TGGTCACCTG GATGGGGTTT TCATGCACAA GAGAGCTCAG 
AGACTCAAGT TCTTGTGTGA GAGGAACGAC AAGGTGTTCT TCGCCTCAGT GCGGTCAGGT 
GGCAGCAGTC AAGTGTTCTT CATGACCCTG AATAGGAACT CTCTGATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163393.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163450.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa07038
Clone ID Related Accession (Same CDS sequence)	XM_005163393.4 , XM_005163393.3
Accession Version	XM_005163393.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATAA ATCATTCAGT TAGCCATATA ACTCACGTTA TCTATTTGTT CATGCATATG 
TTAAGCCACT CGCTTTTCCA TCTTATAAGT GACATCATAA AAGCCCCTTT TTCATGTTTA 
ACCCTGGTTC TGATTCTGAG CCCCATCAGA TGGCTACTGT CCCACTTGGA AGACCTCTTT 
TTTTGTGTTG AGGAGCGATC TAAACTGAAC CGCCAGAGCT CTCCTGCCCT ACAGCATAAA 
GTGGCCAATC GTATCTCTGA CCCTTCCCTC CCGCCTCGCT CTGAGTCCTT CAGCAGTGGA 
GGAATGCAGC CAGCACGCAC CCCTCCTATG CACCGCCCTG TGGAGCCCCA GATGGCTCAT 
TTGGTTCCAG TGAGGTCCCA TAGCTCCTCT ATGTCAGTCT CGCAGTCTTT GCATGATCAA 
TCTGCAAAGG GTGTTTCAGC CTTCCAGGAA AGCCTGGTGT CCAATCGACC AGAGATGCCT 
CGACAGAACT CCGACCCGAC TTCAGAGATG CCCCCTCCTG CCCCTCGCAT GGCCAGCCAT 
GAGGAAAAAC CTGACCGCAG TTCCCCCTGG CTGAGGGAAG ACGCCGTTCC TCCCAAGTTG 
TCCGGTACTA CCCAGGTGCC CCAGAGGACC ACTTCTATCT CCCCTGCTCT TGCTAGGAAA 
AACTCTCCTG GAAACGGAGA GTCCAGTGGA GGGGGGCGGT CGGCCTCTCA GCTCATTCGC 
ACCAGTAATC CAGACCTGAG GAGGACAGAG TTGTCTCTGG ATGTGGCCCT GCAGAGAACC 
AGCAGCAACA GCTCCTCCAG CTCCAGCACA CCCAGCTCTC AGGGTGGATC CCAGCCTGGA 
TCCAGTGAGA GGAACCAGCC AAGAGGATCT GGTAAATCAG AGGGATCTCC AGTGGCATTA 
CAGGAAACCT CCAAGCCCAT TCAGGAGGAA GGCAGAGAGC TAAGCAAACC CAGTCGGCCT 
GCAGATCTGA CTGCCCTGGC TAAAGAACTG AGGGAGCTGC GAATAGATGA GGTCAGTCGA 
CCCCCTCTTA AACTCACAGA CTACTCCTCT TCTAGTGAGG ACTCTGATAG CAGTGAGGAT 
GAGGATGGTA CGGTGGGACA CGACGGCACT GTGTCAGTCA GTGACATACC CAGGATCATG 
CCATCCGTTC AAGGAAATAA TGAGTCGTTC ACGAGCAGCC ATGATGATTC CCTCACTGAC 
TCCTACAATG ATGAGTCTAA AGATGGGACA CTGAGGATGC GAGAGAACAA TGAGAGGCAA 
CGCTTAGGCC ATGGCGAGAG CAATGGCTTT GCAGGCCATG GAAACCTACC AGACCTCCTG 
CAGCAGAGCC ACTCTCCATC CGGAACTCCA ACAGGCCTCA ATTCCCAAGA CCTGGACTCT 
ATGGATGAGT TTGGTCTTGG CGGTGGTTCG AAAGCTTCTT TCACTCCTTT TGTGGACCCA 
CGAGTGTATC AAACCTCCCC CACAGACGAC AACGATGAGA GCTCAGCAGC CGCTATGTTT 
GCGAATGAGC TGTTGCGTCA AGAGCAGGCC AAACTGAATG AGGCCCGCAA GATCTCTGTG 
GTGAACGTCA ACCCCACCAA CATCAGACCT CACAGCGACA CGCCAGAGAT CCGCAAATAC 
AAAAAACGCT TTAACTCCGA GATCTTGTGT GCTGCTCTAT GGGGTGTTAA CCTCTTGGTG 
GGCACTGAAA ATGGTCTGAT GCTGCTGGAT CGTAGCGGTC AGGGCAAGGT TTATAACCTC 
ATCACCCGCA GACGCTTCCA ACAGATGGAT GTGCTGGAGG GTCTAAATGT CCTGGTCACC 
ATATCAGGGA AAAAAAATAA GCTCCGTGTG TACTACTTGT CATGGCTAAG AAACCGAATA 
CTGCACAATG ACCCGGAGGT GGAGAAGAAG CAGGGCTGGA TCACTGTTGG TGAACTCGAA 
GGCTGCATCC ACTATAAAGT TGTGAAATAC GAGAGAATCA AATTCCTGGT GATTGCACTG 
AAGAACTCGG TAGAGATTTA CGCATGGGCC CCCAAGCCGT ACCATAAATT CATGGCTTTC 
AAGTCGTTCA CAGAGCTGCA GCACAGACCT CAGCTGGTGG ACCTCACAGT AGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA CGGCTCTAGT GTGGGCTTTC ACGTCATCGA TGTGGATTCC 
GGAAGCCCCT ACGACATCTA CATACCGTCA CATATTCAGA GCACTGTGAC TCCTCATGCC 
ATCGTGGTGC TTCCGAAGAC TGATGGCATG GAGATGCTGC TGTGTTATGA AGATGAGGGC 
GTTTATGTCA ATACCTACGG CAGAATCACC AAGGACGTTG TCTTGCAGTG GGGAGAGATG 
CCCACCTCTG TTGCTTATAT TCATTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CTGTGGAAAC TGGTCACCTG GATGGGGTTT TCATGCACAA GAGAGCTCAG 
AGACTCAAGT TCTTGTGTGA GAGGAACGAC AAGGTGTTCT TCGCCTCAGT GCGGTCAGGT 
GGCAGCAGTC AAGTGTTCTT CATGACCCTG AATAGGAACT CTCTGATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163450.1
CDS	127..2703
Translation	MYINHSVSHITHVIYLFMHMLSHSLFHLISDIIKAPFSCLTLVLILSPIRWLLSHLEDLFFCVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163393.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163393.3

ORF Insert Sequence:

ATGTACATAA ATCATTCAGT TAGCCATATA ACTCACGTTA TCTATTTGTT CATGCATATG 
TTAAGCCACT CGCTTTTCCA TCTTATAAGT GACATCATAA AAGCCCCTTT TTCATGTTTA 
ACCCTGGTTC TGATTCTGAG CCCCATCAGA TGGCTACTGT CCCACTTGGA AGACCTCTTT 
TTTTGTGTTG AGGAGCGATC TAAACTGAAC CGCCAGAGCT CTCCTGCCCT ACAGCATAAA 
GTGGCCAATC GTATCTCTGA CCCTTCCCTC CCGCCTCGCT CTGAGTCCTT CAGCAGTGGA 
GGAATGCAGC CAGCACGCAC CCCTCCTATG CACCGCCCTG TGGAGCCCCA GATGGCTCAT 
TTGGTTCCAG TGAGGTCCCA TAGCTCCTCT ATGTCAGTCT CGCAGTCTTT GCATGATCAA 
TCTGCAAAGG GTGTTTCAGC CTTCCAGGAA AGCCTGGTGT CCAATCGACC AGAGATGCCT 
CGACAGAACT CCGACCCGAC TTCAGAGATG CCCCCTCCTG CCCCTCGCAT GGCCAGCCAT 
GAGGAAAAAC CTGACCGCAG TTCCCCCTGG CTGAGGGAAG ACGCCGTTCC TCCCAAGTTG 
TCCGGTACTA CCCAGGTGCC CCAGAGGACC ACTTCTATCT CCCCTGCTCT TGCTAGGAAA 
AACTCTCCTG GAAACGGAGA GTCCAGTGGA GGGGGGCGGT CGGCCTCTCA GCTCATTCGC 
ACCAGTAATC CAGACCTGAG GAGGACAGAG TTGTCTCTGG ATGTGGCCCT GCAGAGAACC 
AGCAGCAACA GCTCCTCCAG CTCCAGCACA CCCAGCTCTC AGGGTGGATC CCAGCCTGGA 
TCCAGTGAGA GGAACCAGCC AAGAGGATCT GGTAAATCAG AGGGATCTCC AGTGGCATTA 
CAGGAAACCT CCAAGCCCAT TCAGGAGGAA GGCAGAGAGC TAAGCAAACC CAGTCGGCCT 
GCAGATCTGA CTGCCCTGGC TAAAGAACTG AGGGAGCTGC GAATAGATGA GGTCAGTCGA 
CCCCCTCTTA AACTCACAGA CTACTCCTCT TCTAGTGAGG ACTCTGATAG CAGTGAGGAT 
GAGGATGGTA CGGTGGGACA CGACGGCACT GTGTCAGTCA GTGACATACC CAGGATCATG 
CCATCCGTTC AAGGAAATAA TGAGTCGTTC ACGAGCAGCC ATGATGATTC CCTCACTGAC 
TCCTACAATG ATGAGTCTAA AGATGGGACA CTGAGGATGC GAGAGAACAA TGAGAGGCAA 
CGCTTAGGCC ATGGCGAGAG CAATGGCTTT GCAGGCCATG GAAACCTACC AGACCTCCTG 
CAGCAGAGCC ACTCTCCATC CGGAACTCCA ACAGGCCTCA ATTCCCAAGA CCTGGACTCT 
ATGGATGAGT TTGGTCTTGG CGGTGGTTCG AAAGCTTCTT TCACTCCTTT TGTGGACCCA 
CGAGTGTATC AAACCTCCCC CACAGACGAC AACGATGAGA GCTCAGCAGC CGCTATGTTT 
GCGAATGAGC TGTTGCGTCA AGAGCAGGCC AAACTGAATG AGGCCCGCAA GATCTCTGTG 
GTGAACGTCA ACCCCACCAA CATCAGACCT CACAGCGACA CGCCAGAGAT CCGCAAATAC 
AAAAAACGCT TTAACTCCGA GATCTTGTGT GCTGCTCTAT GGGGTGTTAA CCTCTTGGTG 
GGCACTGAAA ATGGTCTGAT GCTGCTGGAT CGTAGCGGTC AGGGCAAGGT TTATAACCTC 
ATCACCCGCA GACGCTTCCA ACAGATGGAT GTGCTGGAGG GTCTAAATGT CCTGGTCACC 
ATATCAGGGA AAAAAAATAA GCTCCGTGTG TACTACTTGT CATGGCTAAG AAACCGAATA 
CTGCACAATG ACCCGGAGGT GGAGAAGAAG CAGGGCTGGA TCACTGTTGG TGAACTCGAA 
GGCTGCATCC ACTATAAAGT TGTGAAATAC GAGAGAATCA AATTCCTGGT GATTGCACTG 
AAGAACTCGG TAGAGATTTA CGCATGGGCC CCCAAGCCGT ACCATAAATT CATGGCTTTC 
AAGTCGTTCA CAGAGCTGCA GCACAGACCT CAGCTGGTGG ACCTCACAGT AGAAGAGGGT 
CAGAGGTTAA AGGTCATCTA CGGCTCTAGT GTGGGCTTTC ACGTCATCGA TGTGGATTCC 
GGAAGCCCCT ACGACATCTA CATACCGTCA CATATTCAGA GCACTGTGAC TCCTCATGCC 
ATCGTGGTGC TTCCGAAGAC TGATGGCATG GAGATGCTGC TGTGTTATGA AGATGAGGGC 
GTTTATGTCA ATACCTACGG CAGAATCACC AAGGACGTTG TCTTGCAGTG GGGAGAGATG 
CCCACCTCTG TTGCTTATAT TCATTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CTGTGGAAAC TGGTCACCTG GATGGGGTTT TCATGCACAA GAGAGCTCAG 
AGACTCAAGT TCTTGTGTGA GAGGAACGAC AAGGTGTTCT TCGCCTCAGT GCGGTCAGGT 
GGCAGCAGTC AAGTGTTCTT CATGACCCTG AATAGGAACT CTCTGATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_009298543.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296818.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X8, mRNA.

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CloneID	ODa39841
Clone ID Related Accession (Same CDS sequence)	XM_009298543.2
Accession Version	XM_009298543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG CACGCACCCC TCCTATGCAC CGCCCTGTGG AGCCCCAGAT GGCTCATTTG 
GTTCCAGTGA GGTCCCATAG CTCCTCTATG TCAGTCTCGC AGTCTTTGCA TGATCAATCT 
GCAAAGGGTG TTTCAGCCTT CCAGGAAAGC CTGGTGTCCA ATCGACCAGA GATGCCTCGA 
CAGAACTCCG ACCCGACTTC AGAGATGCCC CCTCCTGCCC CTCGCATGGC CAGCCATGAG 
GAAAAACCTG ACCGCAGTTC CCCCTGGCTG AGGGAAGACG CCGTTCCTCC CAAGTTGTCC 
GGTACTACCC AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296818.1
CDS	1152..3425
Translation	MQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_009298543.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298543.2

ORF Insert Sequence:

ATGCAGCCAG CACGCACCCC TCCTATGCAC CGCCCTGTGG AGCCCCAGAT GGCTCATTTG 
GTTCCAGTGA GGTCCCATAG CTCCTCTATG TCAGTCTCGC AGTCTTTGCA TGATCAATCT 
GCAAAGGGTG TTTCAGCCTT CCAGGAAAGC CTGGTGTCCA ATCGACCAGA GATGCCTCGA 
CAGAACTCCG ACCCGACTTC AGAGATGCCC CCTCCTGCCC CTCGCATGGC CAGCCATGAG 
GAAAAACCTG ACCGCAGTTC CCCCTGGCTG AGGGAAGACG CCGTTCCTCC CAAGTTGTCC 
GGTACTACCC AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( NM_199703.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955997.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), mRNA.

Price & Availability↑

CloneID	ODa31229
Clone ID Related Accession (Same CDS sequence)	NM_199703.1
Accession Version	NM_199703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055134.1. ##Evidence-Data-START## Transcript exon combination :: BC055134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GAACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TCCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA GAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAG GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGTGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGCTCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCCGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CTTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGTTGTCCGG TACTACCCAG GTGCCCCAGA GGACCACTTC TATCTCCCCT 
GCTCTTGCTA GGAAAAACTC TCCTGGAAAC GGAGAGTCCA GTGGAGGCGG GCGGTCGGCC 
TCTCAGCTCA TTCGCACCAG TAATCCAGAC CTGAGGAGGA CAGAGTTGTC TCTGGATGTG 
GCCCTGCAGA GAACCAGCAG CAACAGCTCC TCCAGCTCCA GCACACCCAG CTCTCAGGGT 
GGATCCCAGC CTGGATCCAG TGAGAGGAAC CAGCCAAGAG GATCTGGTAA ATCAGAGGGA 
TCTCCAGTGG CATTACAGGA AACTTCCAAG CCCATTCAGG AGGAAGGCAG AGAGCTAAGC 
AAACCCAGTC GGCCTGCAGA TCTGACTGCC CTGGCTAAAG AACTGAGGGA GCTGCGAATA 
GATGAGGTCA GTCGACCCCC TCTTAAACTC ACAGACTACT CCTCTTCTAG TGAGGACTCT 
GATAGCAGTG AGGATGAGGA TGGTACGGTG GGACACGACG GCACTGTGTC AGTCAGTGAC 
ATACCCAGGA TCATGCCCTC CGTTCAAGGA AATAATGAGT CGTTCACGAG CAGCCATGAT 
GATTCCCTCA CTGACTCCTA CAATGATGAG TCTAAAGATG GGACACTGAG GATGCGAGAG 
AACAATGAGA GGCAGCGCTT AGGCCATGGC GAGAGCAATG GCTTTGCAGG CCATGGAAAC 
CTACCAGACC TCCTGCAGCA GAGCCACTCT CCATCCGGGA CTCCAACAGG CCTCAATTCC 
CAAGACCTGG ACTCTATGGA TGAGTTTGGT CTTGGCGGTG GTTCGAAAGC TTCTTTCACT 
CCTTTTGTGG ACCCACGAGT GTATCAAACC TCCCCCACAG ACGACAACGA TGAGAGCTCA 
GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG CGTCAAGAGC AGGCCAAACT GAATGAGGCC 
CGCAAGATCT CTGTGGTGAA CGTCAACCCC ACCAACATCA GACCTCACAG CGACACGCCA 
GAGATCCGCA AATACAAAAA ACGCTTTAAC TCCGAGATCT TGTGTGCTGC TCTATGGGGT 
GTTAACCTCT TGGTGGGCAC TGAAAATGGT CTGATGCTGC TGGATCGTAG CGGTCAGGGT 
AAGGTTTATA ACCTCATCAC CCGCAGACGC TTTCAACAGA TGGATGTGCT GGAGGGTCTA 
AATGTCCTGG TCACCATATC AGGGAAGAGA AATAAGCTCC GTGTGTACTA CTTGTCATGG 
CTAAGAAACC GAATACTGCA CAATGACCCG GAGGTGGAGA AGAAGCAGGG CTGGATCACT 
GTTGGTGAAC TCGAAGGCTG CATCCACTAT AAAGTTGTGA TATACGAGAG AATCAAATTC 
CTGGTGATTG CACTGAAGAA CTCGGTAGAG ATTTACGCAT GGGCCCCCAA GCCGTACCAT 
AAATTCATGG CTTTCAAGTC GTTCACAGAG CTGCAGCACA GACCTCAGCT GGTGGACCTC 
ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC ATCTACGGCT CTAGTGTGGG CTTTCACGTC 
ATCGATGTGG ATTCTGGAAG CCCCTACGAC ATCTACATAC CGTCACATAT TCAGAGCACT 
GTGACTCCTC ATGCCATCGT GGTGCTTCCG AAGACTGATG GCATGGAGAT GCTGCTGCGT 
TATGAAGATG AGGGCGTTTA TGTCAATACC TACGGCAGAA TCACCAAGGA CGTTGTCTTG 
CAGTGGGGAG AGATGCCCAC CTCTGTTGCT TATATTCATT CCAACCAGAC CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCTGTG GAAACTGGTC ACCTGGATGG GGTTTTCATG 
CACAAGAGAG CTCAGAGACT CAAGTTCTTG TGTGAGAGGA ACGACAAGGT GTTCTTCGCC 
TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG TTCTTCATGA CCCTGAATAG GAACTCTCTG 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955997.1
CDS	282..4193
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKRNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVIYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLRYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	NM_199703.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199703.1

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GAACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TCCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA GAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAG GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGTGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGCTCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCCGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CTTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGTTGTCCGG TACTACCCAG GTGCCCCAGA GGACCACTTC TATCTCCCCT 
GCTCTTGCTA GGAAAAACTC TCCTGGAAAC GGAGAGTCCA GTGGAGGCGG GCGGTCGGCC 
TCTCAGCTCA TTCGCACCAG TAATCCAGAC CTGAGGAGGA CAGAGTTGTC TCTGGATGTG 
GCCCTGCAGA GAACCAGCAG CAACAGCTCC TCCAGCTCCA GCACACCCAG CTCTCAGGGT 
GGATCCCAGC CTGGATCCAG TGAGAGGAAC CAGCCAAGAG GATCTGGTAA ATCAGAGGGA 
TCTCCAGTGG CATTACAGGA AACTTCCAAG CCCATTCAGG AGGAAGGCAG AGAGCTAAGC 
AAACCCAGTC GGCCTGCAGA TCTGACTGCC CTGGCTAAAG AACTGAGGGA GCTGCGAATA 
GATGAGGTCA GTCGACCCCC TCTTAAACTC ACAGACTACT CCTCTTCTAG TGAGGACTCT 
GATAGCAGTG AGGATGAGGA TGGTACGGTG GGACACGACG GCACTGTGTC AGTCAGTGAC 
ATACCCAGGA TCATGCCCTC CGTTCAAGGA AATAATGAGT CGTTCACGAG CAGCCATGAT 
GATTCCCTCA CTGACTCCTA CAATGATGAG TCTAAAGATG GGACACTGAG GATGCGAGAG 
AACAATGAGA GGCAGCGCTT AGGCCATGGC GAGAGCAATG GCTTTGCAGG CCATGGAAAC 
CTACCAGACC TCCTGCAGCA GAGCCACTCT CCATCCGGGA CTCCAACAGG CCTCAATTCC 
CAAGACCTGG ACTCTATGGA TGAGTTTGGT CTTGGCGGTG GTTCGAAAGC TTCTTTCACT 
CCTTTTGTGG ACCCACGAGT GTATCAAACC TCCCCCACAG ACGACAACGA TGAGAGCTCA 
GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG CGTCAAGAGC AGGCCAAACT GAATGAGGCC 
CGCAAGATCT CTGTGGTGAA CGTCAACCCC ACCAACATCA GACCTCACAG CGACACGCCA 
GAGATCCGCA AATACAAAAA ACGCTTTAAC TCCGAGATCT TGTGTGCTGC TCTATGGGGT 
GTTAACCTCT TGGTGGGCAC TGAAAATGGT CTGATGCTGC TGGATCGTAG CGGTCAGGGT 
AAGGTTTATA ACCTCATCAC CCGCAGACGC TTTCAACAGA TGGATGTGCT GGAGGGTCTA 
AATGTCCTGG TCACCATATC AGGGAAGAGA AATAAGCTCC GTGTGTACTA CTTGTCATGG 
CTAAGAAACC GAATACTGCA CAATGACCCG GAGGTGGAGA AGAAGCAGGG CTGGATCACT 
GTTGGTGAAC TCGAAGGCTG CATCCACTAT AAAGTTGTGA TATACGAGAG AATCAAATTC 
CTGGTGATTG CACTGAAGAA CTCGGTAGAG ATTTACGCAT GGGCCCCCAA GCCGTACCAT 
AAATTCATGG CTTTCAAGTC GTTCACAGAG CTGCAGCACA GACCTCAGCT GGTGGACCTC 
ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC ATCTACGGCT CTAGTGTGGG CTTTCACGTC 
ATCGATGTGG ATTCTGGAAG CCCCTACGAC ATCTACATAC CGTCACATAT TCAGAGCACT 
GTGACTCCTC ATGCCATCGT GGTGCTTCCG AAGACTGATG GCATGGAGAT GCTGCTGCGT 
TATGAAGATG AGGGCGTTTA TGTCAATACC TACGGCAGAA TCACCAAGGA CGTTGTCTTG 
CAGTGGGGAG AGATGCCCAC CTCTGTTGCT TATATTCATT CCAACCAGAC CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCTGTG GAAACTGGTC ACCTGGATGG GGTTTTCATG 
CACAAGAGAG CTCAGAGACT CAAGTTCTTG TGTGAGAGGA ACGACAAGGT GTTCTTCGCC 
TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG TTCTTCATGA CCCTGAATAG GAACTCTCTG 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163390.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163447.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07035
Clone ID Related Accession (Same CDS sequence)	XM_005163390.4 , XM_005163390.3
Accession Version	XM_005163390.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163390.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGT TGTCCGGTAC TACCCAGGTG 
CCCCAGAGGA CCACTTCTAT CTCCCCTGCT CTTGCTAGGA AAAACTCTCC TGGAAACGGA 
GAGTCCAGTG GAGGGGGGCG GTCGGCCTCT CAGCTCATTC GCACCAGTAA TCCAGACCTG 
AGGAGGACAG AGTTGTCTCT GGATGTGGCC CTGCAGAGAA CCAGCAGCAA CAGCTCCTCC 
AGCTCCAGCA CACCCAGCTC TCAGGGTGGA TCCCAGCCTG GATCCAGTGA GAGGAACCAG 
CCAAGAGGAT CTGGTAAATC AGAGGGATCT CCAGTGGCAT TACAGGAAAC CTCCAAGCCC 
ATTCAGGAGG AAGGCAGAGA GCTAAGCAAA CCCAGTCGGC CTGCAGATCT GACTGCCCTG 
GCTAAAGAAC TGAGGGAGCT GCGAATAGAT GAGGTCAGTC GACCCCCTCT TAAACTCACA 
GACTACTCCT CTTCTAGTGA GGACTCTGAT AGCAGTGAGG ATGAGGATGG TACGGTGGGA 
CACGACGGCA CTGTGTCAGT CAGTGACATA CCCAGGATCA TGCCATCCGT TCAAGGAAAT 
AATGAGTCGT TCACGAGCAG CCATGATGAT TCCCTCACTG ACTCCTACAA TGATGAGTCT 
AAAGATGGGA CACTGAGGAT GCGAGAGAAC AATGAGAGGC AACGCTTAGG CCATGGCGAG 
AGCAATGGCT TTGCAGGCCA TGGAAACCTA CCAGACCTCC TGCAGCAGAG CCACTCTCCA 
TCCGGAACTC CAACAGGCCT CAATTCCCAA GACCTGGACT CTATGGATGA GTTTGGTCTT 
GGCGGTGGTT CGAAAGCTTC TTTCACTCCT TTTGTGGACC CACGAGTGTA TCAAACCTCC 
CCCACAGACG ACAACGATGA GAGCTCAGCA GCCGCTATGT TTGCGAATGA GCTGTTGCGT 
CAAGAGCAGG CCAAACTGAA TGAGGCCCGC AAGATCTCTG TGGTGAACGT CAACCCCACC 
AACATCAGAC CTCACAGCGA CACGCCAGAG ATCCGCAAAT ACAAAAAACG CTTTAACTCC 
GAGATCTTGT GTGCTGCTCT ATGGGGTGTT AACCTCTTGG TGGGCACTGA AAATGGTCTG 
ATGCTGCTGG ATCGTAGCGG TCAGGGCAAG GTTTATAACC TCATCACCCG CAGACGCTTC 
CAACAGATGG ATGTGCTGGA GGGTCTAAAT GTCCTGGTCA CCATATCAGG GAAAAAAAAT 
AAGCTCCGTG TGTACTACTT GTCATGGCTA AGAAACCGAA TACTGCACAA TGACCCGGAG 
GTGGAGAAGA AGCAGGGCTG GATCACTGTT GGTGAACTCG AAGGCTGCAT CCACTATAAA 
GTTGTGAAAT ACGAGAGAAT CAAATTCCTG GTGATTGCAC TGAAGAACTC GGTAGAGATT 
TACGCATGGG CCCCCAAGCC GTACCATAAA TTCATGGCTT TCAAGTCGTT CACAGAGCTG 
CAGCACAGAC CTCAGCTGGT GGACCTCACA GTAGAAGAGG GTCAGAGGTT AAAGGTCATC 
TACGGCTCTA GTGTGGGCTT TCACGTCATC GATGTGGATT CCGGAAGCCC CTACGACATC 
TACATACCGT CACATATTCA GAGCACTGTG ACTCCTCATG CCATCGTGGT GCTTCCGAAG 
ACTGATGGCA TGGAGATGCT GCTGTGTTAT GAAGATGAGG GCGTTTATGT CAATACCTAC 
GGCAGAATCA CCAAGGACGT TGTCTTGCAG TGGGGAGAGA TGCCCACCTC TGTTGCTTAT 
ATTCATTCCA ACCAGATCAT GGGCTGGGGA GAGAAAGCCA TTGAGATCCG CTCTGTGGAA 
ACTGGTCACC TGGATGGGGT TTTCATGCAC AAGAGAGCTC AGAGACTCAA GTTCTTGTGT 
GAGAGGAACG ACAAGGTGTT CTTCGCCTCA GTGCGGTCAG GTGGCAGCAG TCAAGTGTTC 
TTCATGACCC TGAATAGGAA CTCTCTGATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163447.1
CDS	313..4311
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163390.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163390.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGT TGTCCGGTAC TACCCAGGTG 
CCCCAGAGGA CCACTTCTAT CTCCCCTGCT CTTGCTAGGA AAAACTCTCC TGGAAACGGA 
GAGTCCAGTG GAGGGGGGCG GTCGGCCTCT CAGCTCATTC GCACCAGTAA TCCAGACCTG 
AGGAGGACAG AGTTGTCTCT GGATGTGGCC CTGCAGAGAA CCAGCAGCAA CAGCTCCTCC 
AGCTCCAGCA CACCCAGCTC TCAGGGTGGA TCCCAGCCTG GATCCAGTGA GAGGAACCAG 
CCAAGAGGAT CTGGTAAATC AGAGGGATCT CCAGTGGCAT TACAGGAAAC CTCCAAGCCC 
ATTCAGGAGG AAGGCAGAGA GCTAAGCAAA CCCAGTCGGC CTGCAGATCT GACTGCCCTG 
GCTAAAGAAC TGAGGGAGCT GCGAATAGAT GAGGTCAGTC GACCCCCTCT TAAACTCACA 
GACTACTCCT CTTCTAGTGA GGACTCTGAT AGCAGTGAGG ATGAGGATGG TACGGTGGGA 
CACGACGGCA CTGTGTCAGT CAGTGACATA CCCAGGATCA TGCCATCCGT TCAAGGAAAT 
AATGAGTCGT TCACGAGCAG CCATGATGAT TCCCTCACTG ACTCCTACAA TGATGAGTCT 
AAAGATGGGA CACTGAGGAT GCGAGAGAAC AATGAGAGGC AACGCTTAGG CCATGGCGAG 
AGCAATGGCT TTGCAGGCCA TGGAAACCTA CCAGACCTCC TGCAGCAGAG CCACTCTCCA 
TCCGGAACTC CAACAGGCCT CAATTCCCAA GACCTGGACT CTATGGATGA GTTTGGTCTT 
GGCGGTGGTT CGAAAGCTTC TTTCACTCCT TTTGTGGACC CACGAGTGTA TCAAACCTCC 
CCCACAGACG ACAACGATGA GAGCTCAGCA GCCGCTATGT TTGCGAATGA GCTGTTGCGT 
CAAGAGCAGG CCAAACTGAA TGAGGCCCGC AAGATCTCTG TGGTGAACGT CAACCCCACC 
AACATCAGAC CTCACAGCGA CACGCCAGAG ATCCGCAAAT ACAAAAAACG CTTTAACTCC 
GAGATCTTGT GTGCTGCTCT ATGGGGTGTT AACCTCTTGG TGGGCACTGA AAATGGTCTG 
ATGCTGCTGG ATCGTAGCGG TCAGGGCAAG GTTTATAACC TCATCACCCG CAGACGCTTC 
CAACAGATGG ATGTGCTGGA GGGTCTAAAT GTCCTGGTCA CCATATCAGG GAAAAAAAAT 
AAGCTCCGTG TGTACTACTT GTCATGGCTA AGAAACCGAA TACTGCACAA TGACCCGGAG 
GTGGAGAAGA AGCAGGGCTG GATCACTGTT GGTGAACTCG AAGGCTGCAT CCACTATAAA 
GTTGTGAAAT ACGAGAGAAT CAAATTCCTG GTGATTGCAC TGAAGAACTC GGTAGAGATT 
TACGCATGGG CCCCCAAGCC GTACCATAAA TTCATGGCTT TCAAGTCGTT CACAGAGCTG 
CAGCACAGAC CTCAGCTGGT GGACCTCACA GTAGAAGAGG GTCAGAGGTT AAAGGTCATC 
TACGGCTCTA GTGTGGGCTT TCACGTCATC GATGTGGATT CCGGAAGCCC CTACGACATC 
TACATACCGT CACATATTCA GAGCACTGTG ACTCCTCATG CCATCGTGGT GCTTCCGAAG 
ACTGATGGCA TGGAGATGCT GCTGTGTTAT GAAGATGAGG GCGTTTATGT CAATACCTAC 
GGCAGAATCA CCAAGGACGT TGTCTTGCAG TGGGGAGAGA TGCCCACCTC TGTTGCTTAT 
ATTCATTCCA ACCAGATCAT GGGCTGGGGA GAGAAAGCCA TTGAGATCCG CTCTGTGGAA 
ACTGGTCACC TGGATGGGGT TTTCATGCAC AAGAGAGCTC AGAGACTCAA GTTCTTGTGT 
GAGAGGAACG ACAAGGTGTT CTTCGCCTCA GTGCGGTCAG GTGGCAGCAG TCAAGTGTTC 
TTCATGACCC TGAATAGGAA CTCTCTGATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163390.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163447.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07035
Clone ID Related Accession (Same CDS sequence)	XM_005163390.4 , XM_005163390.3
Accession Version	XM_005163390.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGT TGTCCGGTAC TACCCAGGTG 
CCCCAGAGGA CCACTTCTAT CTCCCCTGCT CTTGCTAGGA AAAACTCTCC TGGAAACGGA 
GAGTCCAGTG GAGGGGGGCG GTCGGCCTCT CAGCTCATTC GCACCAGTAA TCCAGACCTG 
AGGAGGACAG AGTTGTCTCT GGATGTGGCC CTGCAGAGAA CCAGCAGCAA CAGCTCCTCC 
AGCTCCAGCA CACCCAGCTC TCAGGGTGGA TCCCAGCCTG GATCCAGTGA GAGGAACCAG 
CCAAGAGGAT CTGGTAAATC AGAGGGATCT CCAGTGGCAT TACAGGAAAC CTCCAAGCCC 
ATTCAGGAGG AAGGCAGAGA GCTAAGCAAA CCCAGTCGGC CTGCAGATCT GACTGCCCTG 
GCTAAAGAAC TGAGGGAGCT GCGAATAGAT GAGGTCAGTC GACCCCCTCT TAAACTCACA 
GACTACTCCT CTTCTAGTGA GGACTCTGAT AGCAGTGAGG ATGAGGATGG TACGGTGGGA 
CACGACGGCA CTGTGTCAGT CAGTGACATA CCCAGGATCA TGCCATCCGT TCAAGGAAAT 
AATGAGTCGT TCACGAGCAG CCATGATGAT TCCCTCACTG ACTCCTACAA TGATGAGTCT 
AAAGATGGGA CACTGAGGAT GCGAGAGAAC AATGAGAGGC AACGCTTAGG CCATGGCGAG 
AGCAATGGCT TTGCAGGCCA TGGAAACCTA CCAGACCTCC TGCAGCAGAG CCACTCTCCA 
TCCGGAACTC CAACAGGCCT CAATTCCCAA GACCTGGACT CTATGGATGA GTTTGGTCTT 
GGCGGTGGTT CGAAAGCTTC TTTCACTCCT TTTGTGGACC CACGAGTGTA TCAAACCTCC 
CCCACAGACG ACAACGATGA GAGCTCAGCA GCCGCTATGT TTGCGAATGA GCTGTTGCGT 
CAAGAGCAGG CCAAACTGAA TGAGGCCCGC AAGATCTCTG TGGTGAACGT CAACCCCACC 
AACATCAGAC CTCACAGCGA CACGCCAGAG ATCCGCAAAT ACAAAAAACG CTTTAACTCC 
GAGATCTTGT GTGCTGCTCT ATGGGGTGTT AACCTCTTGG TGGGCACTGA AAATGGTCTG 
ATGCTGCTGG ATCGTAGCGG TCAGGGCAAG GTTTATAACC TCATCACCCG CAGACGCTTC 
CAACAGATGG ATGTGCTGGA GGGTCTAAAT GTCCTGGTCA CCATATCAGG GAAAAAAAAT 
AAGCTCCGTG TGTACTACTT GTCATGGCTA AGAAACCGAA TACTGCACAA TGACCCGGAG 
GTGGAGAAGA AGCAGGGCTG GATCACTGTT GGTGAACTCG AAGGCTGCAT CCACTATAAA 
GTTGTGAAAT ACGAGAGAAT CAAATTCCTG GTGATTGCAC TGAAGAACTC GGTAGAGATT 
TACGCATGGG CCCCCAAGCC GTACCATAAA TTCATGGCTT TCAAGTCGTT CACAGAGCTG 
CAGCACAGAC CTCAGCTGGT GGACCTCACA GTAGAAGAGG GTCAGAGGTT AAAGGTCATC 
TACGGCTCTA GTGTGGGCTT TCACGTCATC GATGTGGATT CCGGAAGCCC CTACGACATC 
TACATACCGT CACATATTCA GAGCACTGTG ACTCCTCATG CCATCGTGGT GCTTCCGAAG 
ACTGATGGCA TGGAGATGCT GCTGTGTTAT GAAGATGAGG GCGTTTATGT CAATACCTAC 
GGCAGAATCA CCAAGGACGT TGTCTTGCAG TGGGGAGAGA TGCCCACCTC TGTTGCTTAT 
ATTCATTCCA ACCAGATCAT GGGCTGGGGA GAGAAAGCCA TTGAGATCCG CTCTGTGGAA 
ACTGGTCACC TGGATGGGGT TTTCATGCAC AAGAGAGCTC AGAGACTCAA GTTCTTGTGT 
GAGAGGAACG ACAAGGTGTT CTTCGCCTCA GTGCGGTCAG GTGGCAGCAG TCAAGTGTTC 
TTCATGACCC TGAATAGGAA CTCTCTGATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163447.1
CDS	312..4310
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163390.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163390.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGT TGTCCGGTAC TACCCAGGTG 
CCCCAGAGGA CCACTTCTAT CTCCCCTGCT CTTGCTAGGA AAAACTCTCC TGGAAACGGA 
GAGTCCAGTG GAGGGGGGCG GTCGGCCTCT CAGCTCATTC GCACCAGTAA TCCAGACCTG 
AGGAGGACAG AGTTGTCTCT GGATGTGGCC CTGCAGAGAA CCAGCAGCAA CAGCTCCTCC 
AGCTCCAGCA CACCCAGCTC TCAGGGTGGA TCCCAGCCTG GATCCAGTGA GAGGAACCAG 
CCAAGAGGAT CTGGTAAATC AGAGGGATCT CCAGTGGCAT TACAGGAAAC CTCCAAGCCC 
ATTCAGGAGG AAGGCAGAGA GCTAAGCAAA CCCAGTCGGC CTGCAGATCT GACTGCCCTG 
GCTAAAGAAC TGAGGGAGCT GCGAATAGAT GAGGTCAGTC GACCCCCTCT TAAACTCACA 
GACTACTCCT CTTCTAGTGA GGACTCTGAT AGCAGTGAGG ATGAGGATGG TACGGTGGGA 
CACGACGGCA CTGTGTCAGT CAGTGACATA CCCAGGATCA TGCCATCCGT TCAAGGAAAT 
AATGAGTCGT TCACGAGCAG CCATGATGAT TCCCTCACTG ACTCCTACAA TGATGAGTCT 
AAAGATGGGA CACTGAGGAT GCGAGAGAAC AATGAGAGGC AACGCTTAGG CCATGGCGAG 
AGCAATGGCT TTGCAGGCCA TGGAAACCTA CCAGACCTCC TGCAGCAGAG CCACTCTCCA 
TCCGGAACTC CAACAGGCCT CAATTCCCAA GACCTGGACT CTATGGATGA GTTTGGTCTT 
GGCGGTGGTT CGAAAGCTTC TTTCACTCCT TTTGTGGACC CACGAGTGTA TCAAACCTCC 
CCCACAGACG ACAACGATGA GAGCTCAGCA GCCGCTATGT TTGCGAATGA GCTGTTGCGT 
CAAGAGCAGG CCAAACTGAA TGAGGCCCGC AAGATCTCTG TGGTGAACGT CAACCCCACC 
AACATCAGAC CTCACAGCGA CACGCCAGAG ATCCGCAAAT ACAAAAAACG CTTTAACTCC 
GAGATCTTGT GTGCTGCTCT ATGGGGTGTT AACCTCTTGG TGGGCACTGA AAATGGTCTG 
ATGCTGCTGG ATCGTAGCGG TCAGGGCAAG GTTTATAACC TCATCACCCG CAGACGCTTC 
CAACAGATGG ATGTGCTGGA GGGTCTAAAT GTCCTGGTCA CCATATCAGG GAAAAAAAAT 
AAGCTCCGTG TGTACTACTT GTCATGGCTA AGAAACCGAA TACTGCACAA TGACCCGGAG 
GTGGAGAAGA AGCAGGGCTG GATCACTGTT GGTGAACTCG AAGGCTGCAT CCACTATAAA 
GTTGTGAAAT ACGAGAGAAT CAAATTCCTG GTGATTGCAC TGAAGAACTC GGTAGAGATT 
TACGCATGGG CCCCCAAGCC GTACCATAAA TTCATGGCTT TCAAGTCGTT CACAGAGCTG 
CAGCACAGAC CTCAGCTGGT GGACCTCACA GTAGAAGAGG GTCAGAGGTT AAAGGTCATC 
TACGGCTCTA GTGTGGGCTT TCACGTCATC GATGTGGATT CCGGAAGCCC CTACGACATC 
TACATACCGT CACATATTCA GAGCACTGTG ACTCCTCATG CCATCGTGGT GCTTCCGAAG 
ACTGATGGCA TGGAGATGCT GCTGTGTTAT GAAGATGAGG GCGTTTATGT CAATACCTAC 
GGCAGAATCA CCAAGGACGT TGTCTTGCAG TGGGGAGAGA TGCCCACCTC TGTTGCTTAT 
ATTCATTCCA ACCAGATCAT GGGCTGGGGA GAGAAAGCCA TTGAGATCCG CTCTGTGGAA 
ACTGGTCACC TGGATGGGGT TTTCATGCAC AAGAGAGCTC AGAGACTCAA GTTCTTGTGT 
GAGAGGAACG ACAAGGTGTT CTTCGCCTCA GTGCGGTCAG GTGGCAGCAG TCAAGTGTTC 
TTCATGACCC TGAATAGGAA CTCTCTGATG AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163391.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163448.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.

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CloneID	ODa07036
Clone ID Related Accession (Same CDS sequence)	XM_005163391.4 , XM_005163391.3
Accession Version	XM_005163391.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163391.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGG TGCCCCAGAG GACCACTTCT 
ATCTCCCCTG CTCTTGCTAG GAAAAACTCT CCTGGAAACG GAGAGTCCAG TGGAGGGGGG 
CGGTCGGCCT CTCAGCTCAT TCGCACCAGT AATCCAGACC TGAGGAGGAC AGAGTTGTCT 
CTGGATGTGG CCCTGCAGAG AACCAGCAGC AACAGCTCCT CCAGCTCCAG CACACCCAGC 
TCTCAGGGTG GATCCCAGCC TGGATCCAGT GAGAGGAACC AGCCAAGAGG ATCTGGTAAA 
TCAGAGGGAT CTCCAGTGGC ATTACAGGAA ACCTCCAAGC CCATTCAGGA GGAAGGCAGA 
GAGCTAAGCA AACCCAGTCG GCCTGCAGAT CTGACTGCCC TGGCTAAAGA ACTGAGGGAG 
CTGCGAATAG ATGAGGTCAG TCGACCCCCT CTTAAACTCA CAGACTACTC CTCTTCTAGT 
GAGGACTCTG ATAGCAGTGA GGATGAGGAT GGTACGGTGG GACACGACGG CACTGTGTCA 
GTCAGTGACA TACCCAGGAT CATGCCATCC GTTCAAGGAA ATAATGAGTC GTTCACGAGC 
AGCCATGATG ATTCCCTCAC TGACTCCTAC AATGATGAGT CTAAAGATGG GACACTGAGG 
ATGCGAGAGA ACAATGAGAG GCAACGCTTA GGCCATGGCG AGAGCAATGG CTTTGCAGGC 
CATGGAAACC TACCAGACCT CCTGCAGCAG AGCCACTCTC CATCCGGAAC TCCAACAGGC 
CTCAATTCCC AAGACCTGGA CTCTATGGAT GAGTTTGGTC TTGGCGGTGG TTCGAAAGCT 
TCTTTCACTC CTTTTGTGGA CCCACGAGTG TATCAAACCT CCCCCACAGA CGACAACGAT 
GAGAGCTCAG CAGCCGCTAT GTTTGCGAAT GAGCTGTTGC GTCAAGAGCA GGCCAAACTG 
AATGAGGCCC GCAAGATCTC TGTGGTGAAC GTCAACCCCA CCAACATCAG ACCTCACAGC 
GACACGCCAG AGATCCGCAA ATACAAAAAA CGCTTTAACT CCGAGATCTT GTGTGCTGCT 
CTATGGGGTG TTAACCTCTT GGTGGGCACT GAAAATGGTC TGATGCTGCT GGATCGTAGC 
GGTCAGGGCA AGGTTTATAA CCTCATCACC CGCAGACGCT TCCAACAGAT GGATGTGCTG 
GAGGGTCTAA ATGTCCTGGT CACCATATCA GGGAAAAAAA ATAAGCTCCG TGTGTACTAC 
TTGTCATGGC TAAGAAACCG AATACTGCAC AATGACCCGG AGGTGGAGAA GAAGCAGGGC 
TGGATCACTG TTGGTGAACT CGAAGGCTGC ATCCACTATA AAGTTGTGAA ATACGAGAGA 
ATCAAATTCC TGGTGATTGC ACTGAAGAAC TCGGTAGAGA TTTACGCATG GGCCCCCAAG 
CCGTACCATA AATTCATGGC TTTCAAGTCG TTCACAGAGC TGCAGCACAG ACCTCAGCTG 
GTGGACCTCA CAGTAGAAGA GGGTCAGAGG TTAAAGGTCA TCTACGGCTC TAGTGTGGGC 
TTTCACGTCA TCGATGTGGA TTCCGGAAGC CCCTACGACA TCTACATACC GTCACATATT 
CAGAGCACTG TGACTCCTCA TGCCATCGTG GTGCTTCCGA AGACTGATGG CATGGAGATG 
CTGCTGTGTT ATGAAGATGA GGGCGTTTAT GTCAATACCT ACGGCAGAAT CACCAAGGAC 
GTTGTCTTGC AGTGGGGAGA GATGCCCACC TCTGTTGCTT ATATTCATTC CAACCAGATC 
ATGGGCTGGG GAGAGAAAGC CATTGAGATC CGCTCTGTGG AAACTGGTCA CCTGGATGGG 
GTTTTCATGC ACAAGAGAGC TCAGAGACTC AAGTTCTTGT GTGAGAGGAA CGACAAGGTG 
TTCTTCGCCT CAGTGCGGTC AGGTGGCAGC AGTCAAGTGT TCTTCATGAC CCTGAATAGG 
AACTCTCTGA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163448.1
CDS	313..4293
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163391.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163391.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGG TGCCCCAGAG GACCACTTCT 
ATCTCCCCTG CTCTTGCTAG GAAAAACTCT CCTGGAAACG GAGAGTCCAG TGGAGGGGGG 
CGGTCGGCCT CTCAGCTCAT TCGCACCAGT AATCCAGACC TGAGGAGGAC AGAGTTGTCT 
CTGGATGTGG CCCTGCAGAG AACCAGCAGC AACAGCTCCT CCAGCTCCAG CACACCCAGC 
TCTCAGGGTG GATCCCAGCC TGGATCCAGT GAGAGGAACC AGCCAAGAGG ATCTGGTAAA 
TCAGAGGGAT CTCCAGTGGC ATTACAGGAA ACCTCCAAGC CCATTCAGGA GGAAGGCAGA 
GAGCTAAGCA AACCCAGTCG GCCTGCAGAT CTGACTGCCC TGGCTAAAGA ACTGAGGGAG 
CTGCGAATAG ATGAGGTCAG TCGACCCCCT CTTAAACTCA CAGACTACTC CTCTTCTAGT 
GAGGACTCTG ATAGCAGTGA GGATGAGGAT GGTACGGTGG GACACGACGG CACTGTGTCA 
GTCAGTGACA TACCCAGGAT CATGCCATCC GTTCAAGGAA ATAATGAGTC GTTCACGAGC 
AGCCATGATG ATTCCCTCAC TGACTCCTAC AATGATGAGT CTAAAGATGG GACACTGAGG 
ATGCGAGAGA ACAATGAGAG GCAACGCTTA GGCCATGGCG AGAGCAATGG CTTTGCAGGC 
CATGGAAACC TACCAGACCT CCTGCAGCAG AGCCACTCTC CATCCGGAAC TCCAACAGGC 
CTCAATTCCC AAGACCTGGA CTCTATGGAT GAGTTTGGTC TTGGCGGTGG TTCGAAAGCT 
TCTTTCACTC CTTTTGTGGA CCCACGAGTG TATCAAACCT CCCCCACAGA CGACAACGAT 
GAGAGCTCAG CAGCCGCTAT GTTTGCGAAT GAGCTGTTGC GTCAAGAGCA GGCCAAACTG 
AATGAGGCCC GCAAGATCTC TGTGGTGAAC GTCAACCCCA CCAACATCAG ACCTCACAGC 
GACACGCCAG AGATCCGCAA ATACAAAAAA CGCTTTAACT CCGAGATCTT GTGTGCTGCT 
CTATGGGGTG TTAACCTCTT GGTGGGCACT GAAAATGGTC TGATGCTGCT GGATCGTAGC 
GGTCAGGGCA AGGTTTATAA CCTCATCACC CGCAGACGCT TCCAACAGAT GGATGTGCTG 
GAGGGTCTAA ATGTCCTGGT CACCATATCA GGGAAAAAAA ATAAGCTCCG TGTGTACTAC 
TTGTCATGGC TAAGAAACCG AATACTGCAC AATGACCCGG AGGTGGAGAA GAAGCAGGGC 
TGGATCACTG TTGGTGAACT CGAAGGCTGC ATCCACTATA AAGTTGTGAA ATACGAGAGA 
ATCAAATTCC TGGTGATTGC ACTGAAGAAC TCGGTAGAGA TTTACGCATG GGCCCCCAAG 
CCGTACCATA AATTCATGGC TTTCAAGTCG TTCACAGAGC TGCAGCACAG ACCTCAGCTG 
GTGGACCTCA CAGTAGAAGA GGGTCAGAGG TTAAAGGTCA TCTACGGCTC TAGTGTGGGC 
TTTCACGTCA TCGATGTGGA TTCCGGAAGC CCCTACGACA TCTACATACC GTCACATATT 
CAGAGCACTG TGACTCCTCA TGCCATCGTG GTGCTTCCGA AGACTGATGG CATGGAGATG 
CTGCTGTGTT ATGAAGATGA GGGCGTTTAT GTCAATACCT ACGGCAGAAT CACCAAGGAC 
GTTGTCTTGC AGTGGGGAGA GATGCCCACC TCTGTTGCTT ATATTCATTC CAACCAGATC 
ATGGGCTGGG GAGAGAAAGC CATTGAGATC CGCTCTGTGG AAACTGGTCA CCTGGATGGG 
GTTTTCATGC ACAAGAGAGC TCAGAGACTC AAGTTCTTGT GTGAGAGGAA CGACAAGGTG 
TTCTTCGCCT CAGTGCGGTC AGGTGGCAGC AGTCAAGTGT TCTTCATGAC CCTGAATAGG 
AACTCTCTGA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163391.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163448.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.

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CloneID	ODa07036
Clone ID Related Accession (Same CDS sequence)	XM_005163391.4 , XM_005163391.3
Accession Version	XM_005163391.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGG TGCCCCAGAG GACCACTTCT 
ATCTCCCCTG CTCTTGCTAG GAAAAACTCT CCTGGAAACG GAGAGTCCAG TGGAGGGGGG 
CGGTCGGCCT CTCAGCTCAT TCGCACCAGT AATCCAGACC TGAGGAGGAC AGAGTTGTCT 
CTGGATGTGG CCCTGCAGAG AACCAGCAGC AACAGCTCCT CCAGCTCCAG CACACCCAGC 
TCTCAGGGTG GATCCCAGCC TGGATCCAGT GAGAGGAACC AGCCAAGAGG ATCTGGTAAA 
TCAGAGGGAT CTCCAGTGGC ATTACAGGAA ACCTCCAAGC CCATTCAGGA GGAAGGCAGA 
GAGCTAAGCA AACCCAGTCG GCCTGCAGAT CTGACTGCCC TGGCTAAAGA ACTGAGGGAG 
CTGCGAATAG ATGAGGTCAG TCGACCCCCT CTTAAACTCA CAGACTACTC CTCTTCTAGT 
GAGGACTCTG ATAGCAGTGA GGATGAGGAT GGTACGGTGG GACACGACGG CACTGTGTCA 
GTCAGTGACA TACCCAGGAT CATGCCATCC GTTCAAGGAA ATAATGAGTC GTTCACGAGC 
AGCCATGATG ATTCCCTCAC TGACTCCTAC AATGATGAGT CTAAAGATGG GACACTGAGG 
ATGCGAGAGA ACAATGAGAG GCAACGCTTA GGCCATGGCG AGAGCAATGG CTTTGCAGGC 
CATGGAAACC TACCAGACCT CCTGCAGCAG AGCCACTCTC CATCCGGAAC TCCAACAGGC 
CTCAATTCCC AAGACCTGGA CTCTATGGAT GAGTTTGGTC TTGGCGGTGG TTCGAAAGCT 
TCTTTCACTC CTTTTGTGGA CCCACGAGTG TATCAAACCT CCCCCACAGA CGACAACGAT 
GAGAGCTCAG CAGCCGCTAT GTTTGCGAAT GAGCTGTTGC GTCAAGAGCA GGCCAAACTG 
AATGAGGCCC GCAAGATCTC TGTGGTGAAC GTCAACCCCA CCAACATCAG ACCTCACAGC 
GACACGCCAG AGATCCGCAA ATACAAAAAA CGCTTTAACT CCGAGATCTT GTGTGCTGCT 
CTATGGGGTG TTAACCTCTT GGTGGGCACT GAAAATGGTC TGATGCTGCT GGATCGTAGC 
GGTCAGGGCA AGGTTTATAA CCTCATCACC CGCAGACGCT TCCAACAGAT GGATGTGCTG 
GAGGGTCTAA ATGTCCTGGT CACCATATCA GGGAAAAAAA ATAAGCTCCG TGTGTACTAC 
TTGTCATGGC TAAGAAACCG AATACTGCAC AATGACCCGG AGGTGGAGAA GAAGCAGGGC 
TGGATCACTG TTGGTGAACT CGAAGGCTGC ATCCACTATA AAGTTGTGAA ATACGAGAGA 
ATCAAATTCC TGGTGATTGC ACTGAAGAAC TCGGTAGAGA TTTACGCATG GGCCCCCAAG 
CCGTACCATA AATTCATGGC TTTCAAGTCG TTCACAGAGC TGCAGCACAG ACCTCAGCTG 
GTGGACCTCA CAGTAGAAGA GGGTCAGAGG TTAAAGGTCA TCTACGGCTC TAGTGTGGGC 
TTTCACGTCA TCGATGTGGA TTCCGGAAGC CCCTACGACA TCTACATACC GTCACATATT 
CAGAGCACTG TGACTCCTCA TGCCATCGTG GTGCTTCCGA AGACTGATGG CATGGAGATG 
CTGCTGTGTT ATGAAGATGA GGGCGTTTAT GTCAATACCT ACGGCAGAAT CACCAAGGAC 
GTTGTCTTGC AGTGGGGAGA GATGCCCACC TCTGTTGCTT ATATTCATTC CAACCAGATC 
ATGGGCTGGG GAGAGAAAGC CATTGAGATC CGCTCTGTGG AAACTGGTCA CCTGGATGGG 
GTTTTCATGC ACAAGAGAGC TCAGAGACTC AAGTTCTTGT GTGAGAGGAA CGACAAGGTG 
TTCTTCGCCT CAGTGCGGTC AGGTGGCAGC AGTCAAGTGT TCTTCATGAC CCTGAATAGG 
AACTCTCTGA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163448.1
CDS	312..4292
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163391.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163391.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGGT TGAGGAGCGA 
TCTAAACTGA ACCGCCAGAG CTCTCCTGCC CTACAGCATA AAGTGGCCAA TCGTATCTCT 
GACCCTTCCC TCCCGCCTCG CTCTGAGTCC TTCAGCAGTG GAGGAATGCA GCCAGCACGC 
ACCCCTCCTA TGCACCGCCC TGTGGAGCCC CAGATGGCTC ATTTGGTTCC AGTGAGGTCC 
CATAGCTCCT CTATGTCAGT CTCGCAGTCT TTGCATGATC AATCTGCAAA GGGTGTTTCA 
GCCTTCCAGG AAAGCCTGGT GTCCAATCGA CCAGAGATGC CTCGACAGAA CTCCGACCCG 
ACTTCAGAGA TGCCCCCTCC TGCCCCTCGC ATGGCCAGCC ATGAGGAAAA ACCTGACCGC 
AGTTCCCCCT GGCTGAGGGA AGACGCCGTT CCTCCCAAGG TGCCCCAGAG GACCACTTCT 
ATCTCCCCTG CTCTTGCTAG GAAAAACTCT CCTGGAAACG GAGAGTCCAG TGGAGGGGGG 
CGGTCGGCCT CTCAGCTCAT TCGCACCAGT AATCCAGACC TGAGGAGGAC AGAGTTGTCT 
CTGGATGTGG CCCTGCAGAG AACCAGCAGC AACAGCTCCT CCAGCTCCAG CACACCCAGC 
TCTCAGGGTG GATCCCAGCC TGGATCCAGT GAGAGGAACC AGCCAAGAGG ATCTGGTAAA 
TCAGAGGGAT CTCCAGTGGC ATTACAGGAA ACCTCCAAGC CCATTCAGGA GGAAGGCAGA 
GAGCTAAGCA AACCCAGTCG GCCTGCAGAT CTGACTGCCC TGGCTAAAGA ACTGAGGGAG 
CTGCGAATAG ATGAGGTCAG TCGACCCCCT CTTAAACTCA CAGACTACTC CTCTTCTAGT 
GAGGACTCTG ATAGCAGTGA GGATGAGGAT GGTACGGTGG GACACGACGG CACTGTGTCA 
GTCAGTGACA TACCCAGGAT CATGCCATCC GTTCAAGGAA ATAATGAGTC GTTCACGAGC 
AGCCATGATG ATTCCCTCAC TGACTCCTAC AATGATGAGT CTAAAGATGG GACACTGAGG 
ATGCGAGAGA ACAATGAGAG GCAACGCTTA GGCCATGGCG AGAGCAATGG CTTTGCAGGC 
CATGGAAACC TACCAGACCT CCTGCAGCAG AGCCACTCTC CATCCGGAAC TCCAACAGGC 
CTCAATTCCC AAGACCTGGA CTCTATGGAT GAGTTTGGTC TTGGCGGTGG TTCGAAAGCT 
TCTTTCACTC CTTTTGTGGA CCCACGAGTG TATCAAACCT CCCCCACAGA CGACAACGAT 
GAGAGCTCAG CAGCCGCTAT GTTTGCGAAT GAGCTGTTGC GTCAAGAGCA GGCCAAACTG 
AATGAGGCCC GCAAGATCTC TGTGGTGAAC GTCAACCCCA CCAACATCAG ACCTCACAGC 
GACACGCCAG AGATCCGCAA ATACAAAAAA CGCTTTAACT CCGAGATCTT GTGTGCTGCT 
CTATGGGGTG TTAACCTCTT GGTGGGCACT GAAAATGGTC TGATGCTGCT GGATCGTAGC 
GGTCAGGGCA AGGTTTATAA CCTCATCACC CGCAGACGCT TCCAACAGAT GGATGTGCTG 
GAGGGTCTAA ATGTCCTGGT CACCATATCA GGGAAAAAAA ATAAGCTCCG TGTGTACTAC 
TTGTCATGGC TAAGAAACCG AATACTGCAC AATGACCCGG AGGTGGAGAA GAAGCAGGGC 
TGGATCACTG TTGGTGAACT CGAAGGCTGC ATCCACTATA AAGTTGTGAA ATACGAGAGA 
ATCAAATTCC TGGTGATTGC ACTGAAGAAC TCGGTAGAGA TTTACGCATG GGCCCCCAAG 
CCGTACCATA AATTCATGGC TTTCAAGTCG TTCACAGAGC TGCAGCACAG ACCTCAGCTG 
GTGGACCTCA CAGTAGAAGA GGGTCAGAGG TTAAAGGTCA TCTACGGCTC TAGTGTGGGC 
TTTCACGTCA TCGATGTGGA TTCCGGAAGC CCCTACGACA TCTACATACC GTCACATATT 
CAGAGCACTG TGACTCCTCA TGCCATCGTG GTGCTTCCGA AGACTGATGG CATGGAGATG 
CTGCTGTGTT ATGAAGATGA GGGCGTTTAT GTCAATACCT ACGGCAGAAT CACCAAGGAC 
GTTGTCTTGC AGTGGGGAGA GATGCCCACC TCTGTTGCTT ATATTCATTC CAACCAGATC 
ATGGGCTGGG GAGAGAAAGC CATTGAGATC CGCTCTGTGG AAACTGGTCA CCTGGATGGG 
GTTTTCATGC ACAAGAGAGC TCAGAGACTC AAGTTCTTGT GTGAGAGGAA CGACAAGGTG 
TTCTTCGCCT CAGTGCGGTC AGGTGGCAGC AGTCAAGTGT TCTTCATGAC CCTGAATAGG 
AACTCTCTGA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163392.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163449.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa07037
Clone ID Related Accession (Same CDS sequence)	XM_005163392.4 , XM_005163392.3
Accession Version	XM_005163392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163392.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGATCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCTGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CCTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163449.1
CDS	313..4206
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163392.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163392.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGATCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCTGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CCTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163392.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163449.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa07037
Clone ID Related Accession (Same CDS sequence)	XM_005163392.4 , XM_005163392.3
Accession Version	XM_005163392.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGATCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCTGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CCTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163449.1
CDS	312..4205
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163392.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163392.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GGTTGAGGAG CGATCTAAAC TGAACCGCCA GAGCTCTCCT 
GCCCTACAGC ATAAAGTGGC CAATCGTATC TCTGACCCTT CCCTCCCGCC TCGCTCTGAG 
TCCTTCAGCA GTGGAGGAAT GCAGCCAGCA CGCACCCCTC CTATGCACCG CCCTGTGGAG 
CCCCAGATGG CTCATTTGGT TCCAGTGAGG TCCCATAGCT CCTCTATGTC AGTCTCGCAG 
TCTTTGCATG ATCAATCTGC AAAGGGTGTT TCAGCCTTCC AGGAAAGCCT GGTGTCCAAT 
CGACCAGAGA TGCCTCGACA GAACTCCGAC CCGACTTCAG AGATGCCCCC TCCTGCCCCT 
CGCATGGCCA GCCATGAGGA AAAACCTGAC CGCAGTTCCC CCTGGCTGAG GGAAGACGCC 
GTTCCTCCCA AGGTGCCCCA GAGGACCACT TCTATCTCCC CTGCTCTTGC TAGGAAAAAC 
TCTCCTGGAA ACGGAGAGTC CAGTGGAGGG GGGCGGTCGG CCTCTCAGCT CATTCGCACC 
AGTAATCCAG ACCTGAGGAG GACAGAGTTG TCTCTGGATG TGGCCCTGCA GAGAACCAGC 
AGCAACAGCT CCTCCAGCTC CAGCACACCC AGCTCTCAGG GTGGATCCCA GCCTGGATCC 
AGTGAGAGGA ACCAGCCAAG AGGATCTGGT AAATCAGAGG GATCTCCAGT GGCATTACAG 
GAAACCTCCA AGCCCATTCA GGAGGAAGGC AGAGAGCTAA GCAAACCCAG TCGGCCTGCA 
GATCTGACTG CCCTGGCTAA AGAACTGAGG GAGCTGCGAA TAGATGAGGT CAGTCGACCC 
CCTCTTAAAC TCACAGACTA CTCCTCTTCT AGTGAGGACT CTGATAGCAG TGAGGATGAG 
GATGGTACGG TGGGACACGA CGGCACTGTG TCAGTCAGTG ACATACCCAG GATCATGCCA 
TCCGTTCAAG GAAATAATGA GTCGTTCACG AGCAGCCATG ATGATTCCCT CACTGACTCC 
TACAATGATG AGTCTAAAGA TGGGACACTG AGGATGCGAG AGAACAATGA GAGGCAACGC 
TTAGGCCATG GCGAGAGCAA TGGCTTTGCA GGCCATGGAA ACCTACCAGA CCTCCTGCAG 
CAGAGCCACT CTCCATCCGG AACTCCAACA GGCCTCAATT CCCAAGACCT GGACTCTATG 
GATGAGTTTG GTCTTGGCGG TGGTTCGAAA GCTTCTTTCA CTCCTTTTGT GGACCCACGA 
GTGTATCAAA CCTCCCCCAC AGACGACAAC GATGAGAGCT CAGCAGCCGC TATGTTTGCG 
AATGAGCTGT TGCGTCAAGA GCAGGCCAAA CTGAATGAGG CCCGCAAGAT CTCTGTGGTG 
AACGTCAACC CCACCAACAT CAGACCTCAC AGCGACACGC CAGAGATCCG CAAATACAAA 
AAACGCTTTA ACTCCGAGAT CTTGTGTGCT GCTCTATGGG GTGTTAACCT CTTGGTGGGC 
ACTGAAAATG GTCTGATGCT GCTGGATCGT AGCGGTCAGG GCAAGGTTTA TAACCTCATC 
ACCCGCAGAC GCTTCCAACA GATGGATGTG CTGGAGGGTC TAAATGTCCT GGTCACCATA 
TCAGGGAAAA AAAATAAGCT CCGTGTGTAC TACTTGTCAT GGCTAAGAAA CCGAATACTG 
CACAATGACC CGGAGGTGGA GAAGAAGCAG GGCTGGATCA CTGTTGGTGA ACTCGAAGGC 
TGCATCCACT ATAAAGTTGT GAAATACGAG AGAATCAAAT TCCTGGTGAT TGCACTGAAG 
AACTCGGTAG AGATTTACGC ATGGGCCCCC AAGCCGTACC ATAAATTCAT GGCTTTCAAG 
TCGTTCACAG AGCTGCAGCA CAGACCTCAG CTGGTGGACC TCACAGTAGA AGAGGGTCAG 
AGGTTAAAGG TCATCTACGG CTCTAGTGTG GGCTTTCACG TCATCGATGT GGATTCCGGA 
AGCCCCTACG ACATCTACAT ACCGTCACAT ATTCAGAGCA CTGTGACTCC TCATGCCATC 
GTGGTGCTTC CGAAGACTGA TGGCATGGAG ATGCTGCTGT GTTATGAAGA TGAGGGCGTT 
TATGTCAATA CCTACGGCAG AATCACCAAG GACGTTGTCT TGCAGTGGGG AGAGATGCCC 
ACCTCTGTTG CTTATATTCA TTCCAACCAG ATCATGGGCT GGGGAGAGAA AGCCATTGAG 
ATCCGCTCTG TGGAAACTGG TCACCTGGAT GGGGTTTTCA TGCACAAGAG AGCTCAGAGA 
CTCAAGTTCT TGTGTGAGAG GAACGACAAG GTGTTCTTCG CCTCAGTGCG GTCAGGTGGC 
AGCAGTCAAG TGTTCTTCAT GACCCTGAAT AGGAACTCTC TGATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163387.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163444.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.

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CloneID	ODa07032
Clone ID Related Accession (Same CDS sequence)	XM_005163387.3 , XM_005163387.4
Accession Version	XM_005163387.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
TTGTCCGGTA CTACCCAGGT GCCCCAGAGG ACCACTTCTA TCTCCCCTGC TCTTGCTAGG 
AAAAACTCTC CTGGAAACGG AGAGTCCAGT GGAGGGGGGC GGTCGGCCTC TCAGCTCATT 
CGCACCAGTA ATCCAGACCT GAGGAGGACA GAGTTGTCTC TGGATGTGGC CCTGCAGAGA 
ACCAGCAGCA ACAGCTCCTC CAGCTCCAGC ACACCCAGCT CTCAGGGTGG ATCCCAGCCT 
GGATCCAGTG AGAGGAACCA GCCAAGAGGA TCTGGTAAAT CAGAGGGATC TCCAGTGGCA 
TTACAGGAAA CCTCCAAGCC CATTCAGGAG GAAGGCAGAG AGCTAAGCAA ACCCAGTCGG 
CCTGCAGATC TGACTGCCCT GGCTAAAGAA CTGAGGGAGC TGCGAATAGA TGAGGTCAGT 
CGACCCCCTC TTAAACTCAC AGACTACTCC TCTTCTAGTG AGGACTCTGA TAGCAGTGAG 
GATGAGGATG GTACGGTGGG ACACGACGGC ACTGTGTCAG TCAGTGACAT ACCCAGGATC 
ATGCCATCCG TTCAAGGAAA TAATGAGTCG TTCACGAGCA GCCATGATGA TTCCCTCACT 
GACTCCTACA ATGATGAGTC TAAAGATGGG ACACTGAGGA TGCGAGAGAA CAATGAGAGG 
CAACGCTTAG GCCATGGCGA GAGCAATGGC TTTGCAGGCC ATGGAAACCT ACCAGACCTC 
CTGCAGCAGA GCCACTCTCC ATCCGGAACT CCAACAGGCC TCAATTCCCA AGACCTGGAC 
TCTATGGATG AGTTTGGTCT TGGCGGTGGT TCGAAAGCTT CTTTCACTCC TTTTGTGGAC 
CCACGAGTGT ATCAAACCTC CCCCACAGAC GACAACGATG AGAGCTCAGC AGCCGCTATG 
TTTGCGAATG AGCTGTTGCG TCAAGAGCAG GCCAAACTGA ATGAGGCCCG CAAGATCTCT 
GTGGTGAACG TCAACCCCAC CAACATCAGA CCTCACAGCG ACACGCCAGA GATCCGCAAA 
TACAAAAAAC GCTTTAACTC CGAGATCTTG TGTGCTGCTC TATGGGGTGT TAACCTCTTG 
GTGGGCACTG AAAATGGTCT GATGCTGCTG GATCGTAGCG GTCAGGGCAA GGTTTATAAC 
CTCATCACCC GCAGACGCTT CCAACAGATG GATGTGCTGG AGGGTCTAAA TGTCCTGGTC 
ACCATATCAG GGAAAAAAAA TAAGCTCCGT GTGTACTACT TGTCATGGCT AAGAAACCGA 
ATACTGCACA ATGACCCGGA GGTGGAGAAG AAGCAGGGCT GGATCACTGT TGGTGAACTC 
GAAGGCTGCA TCCACTATAA AGTTGTGAAA TACGAGAGAA TCAAATTCCT GGTGATTGCA 
CTGAAGAACT CGGTAGAGAT TTACGCATGG GCCCCCAAGC CGTACCATAA ATTCATGGCT 
TTCAAGTCGT TCACAGAGCT GCAGCACAGA CCTCAGCTGG TGGACCTCAC AGTAGAAGAG 
GGTCAGAGGT TAAAGGTCAT CTACGGCTCT AGTGTGGGCT TTCACGTCAT CGATGTGGAT 
TCCGGAAGCC CCTACGACAT CTACATACCG TCACATATTC AGAGCACTGT GACTCCTCAT 
GCCATCGTGG TGCTTCCGAA GACTGATGGC ATGGAGATGC TGCTGTGTTA TGAAGATGAG 
GGCGTTTATG TCAATACCTA CGGCAGAATC ACCAAGGACG TTGTCTTGCA GTGGGGAGAG 
ATGCCCACCT CTGTTGCTTA TATTCATTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCTGTGGA AACTGGTCAC CTGGATGGGG TTTTCATGCA CAAGAGAGCT 
CAGAGACTCA AGTTCTTGTG TGAGAGGAAC GACAAGGTGT TCTTCGCCTC AGTGCGGTCA 
GGTGGCAGCA GTCAAGTGTT CTTCATGACC CTGAATAGGA ACTCTCTGAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163444.1
CDS	312..4571
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163387.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163387.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
TTGTCCGGTA CTACCCAGGT GCCCCAGAGG ACCACTTCTA TCTCCCCTGC TCTTGCTAGG 
AAAAACTCTC CTGGAAACGG AGAGTCCAGT GGAGGGGGGC GGTCGGCCTC TCAGCTCATT 
CGCACCAGTA ATCCAGACCT GAGGAGGACA GAGTTGTCTC TGGATGTGGC CCTGCAGAGA 
ACCAGCAGCA ACAGCTCCTC CAGCTCCAGC ACACCCAGCT CTCAGGGTGG ATCCCAGCCT 
GGATCCAGTG AGAGGAACCA GCCAAGAGGA TCTGGTAAAT CAGAGGGATC TCCAGTGGCA 
TTACAGGAAA CCTCCAAGCC CATTCAGGAG GAAGGCAGAG AGCTAAGCAA ACCCAGTCGG 
CCTGCAGATC TGACTGCCCT GGCTAAAGAA CTGAGGGAGC TGCGAATAGA TGAGGTCAGT 
CGACCCCCTC TTAAACTCAC AGACTACTCC TCTTCTAGTG AGGACTCTGA TAGCAGTGAG 
GATGAGGATG GTACGGTGGG ACACGACGGC ACTGTGTCAG TCAGTGACAT ACCCAGGATC 
ATGCCATCCG TTCAAGGAAA TAATGAGTCG TTCACGAGCA GCCATGATGA TTCCCTCACT 
GACTCCTACA ATGATGAGTC TAAAGATGGG ACACTGAGGA TGCGAGAGAA CAATGAGAGG 
CAACGCTTAG GCCATGGCGA GAGCAATGGC TTTGCAGGCC ATGGAAACCT ACCAGACCTC 
CTGCAGCAGA GCCACTCTCC ATCCGGAACT CCAACAGGCC TCAATTCCCA AGACCTGGAC 
TCTATGGATG AGTTTGGTCT TGGCGGTGGT TCGAAAGCTT CTTTCACTCC TTTTGTGGAC 
CCACGAGTGT ATCAAACCTC CCCCACAGAC GACAACGATG AGAGCTCAGC AGCCGCTATG 
TTTGCGAATG AGCTGTTGCG TCAAGAGCAG GCCAAACTGA ATGAGGCCCG CAAGATCTCT 
GTGGTGAACG TCAACCCCAC CAACATCAGA CCTCACAGCG ACACGCCAGA GATCCGCAAA 
TACAAAAAAC GCTTTAACTC CGAGATCTTG TGTGCTGCTC TATGGGGTGT TAACCTCTTG 
GTGGGCACTG AAAATGGTCT GATGCTGCTG GATCGTAGCG GTCAGGGCAA GGTTTATAAC 
CTCATCACCC GCAGACGCTT CCAACAGATG GATGTGCTGG AGGGTCTAAA TGTCCTGGTC 
ACCATATCAG GGAAAAAAAA TAAGCTCCGT GTGTACTACT TGTCATGGCT AAGAAACCGA 
ATACTGCACA ATGACCCGGA GGTGGAGAAG AAGCAGGGCT GGATCACTGT TGGTGAACTC 
GAAGGCTGCA TCCACTATAA AGTTGTGAAA TACGAGAGAA TCAAATTCCT GGTGATTGCA 
CTGAAGAACT CGGTAGAGAT TTACGCATGG GCCCCCAAGC CGTACCATAA ATTCATGGCT 
TTCAAGTCGT TCACAGAGCT GCAGCACAGA CCTCAGCTGG TGGACCTCAC AGTAGAAGAG 
GGTCAGAGGT TAAAGGTCAT CTACGGCTCT AGTGTGGGCT TTCACGTCAT CGATGTGGAT 
TCCGGAAGCC CCTACGACAT CTACATACCG TCACATATTC AGAGCACTGT GACTCCTCAT 
GCCATCGTGG TGCTTCCGAA GACTGATGGC ATGGAGATGC TGCTGTGTTA TGAAGATGAG 
GGCGTTTATG TCAATACCTA CGGCAGAATC ACCAAGGACG TTGTCTTGCA GTGGGGAGAG 
ATGCCCACCT CTGTTGCTTA TATTCATTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCTGTGGA AACTGGTCAC CTGGATGGGG TTTTCATGCA CAAGAGAGCT 
CAGAGACTCA AGTTCTTGTG TGAGAGGAAC GACAAGGTGT TCTTCGCCTC AGTGCGGTCA 
GGTGGCAGCA GTCAAGTGTT CTTCATGACC CTGAATAGGA ACTCTCTGAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163387.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163444.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07032
Clone ID Related Accession (Same CDS sequence)	XM_005163387.3 , XM_005163387.4
Accession Version	XM_005163387.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163387.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
TTGTCCGGTA CTACCCAGGT GCCCCAGAGG ACCACTTCTA TCTCCCCTGC TCTTGCTAGG 
AAAAACTCTC CTGGAAACGG AGAGTCCAGT GGAGGGGGGC GGTCGGCCTC TCAGCTCATT 
CGCACCAGTA ATCCAGACCT GAGGAGGACA GAGTTGTCTC TGGATGTGGC CCTGCAGAGA 
ACCAGCAGCA ACAGCTCCTC CAGCTCCAGC ACACCCAGCT CTCAGGGTGG ATCCCAGCCT 
GGATCCAGTG AGAGGAACCA GCCAAGAGGA TCTGGTAAAT CAGAGGGATC TCCAGTGGCA 
TTACAGGAAA CCTCCAAGCC CATTCAGGAG GAAGGCAGAG AGCTAAGCAA ACCCAGTCGG 
CCTGCAGATC TGACTGCCCT GGCTAAAGAA CTGAGGGAGC TGCGAATAGA TGAGGTCAGT 
CGACCCCCTC TTAAACTCAC AGACTACTCC TCTTCTAGTG AGGACTCTGA TAGCAGTGAG 
GATGAGGATG GTACGGTGGG ACACGACGGC ACTGTGTCAG TCAGTGACAT ACCCAGGATC 
ATGCCATCCG TTCAAGGAAA TAATGAGTCG TTCACGAGCA GCCATGATGA TTCCCTCACT 
GACTCCTACA ATGATGAGTC TAAAGATGGG ACACTGAGGA TGCGAGAGAA CAATGAGAGG 
CAACGCTTAG GCCATGGCGA GAGCAATGGC TTTGCAGGCC ATGGAAACCT ACCAGACCTC 
CTGCAGCAGA GCCACTCTCC ATCCGGAACT CCAACAGGCC TCAATTCCCA AGACCTGGAC 
TCTATGGATG AGTTTGGTCT TGGCGGTGGT TCGAAAGCTT CTTTCACTCC TTTTGTGGAC 
CCACGAGTGT ATCAAACCTC CCCCACAGAC GACAACGATG AGAGCTCAGC AGCCGCTATG 
TTTGCGAATG AGCTGTTGCG TCAAGAGCAG GCCAAACTGA ATGAGGCCCG CAAGATCTCT 
GTGGTGAACG TCAACCCCAC CAACATCAGA CCTCACAGCG ACACGCCAGA GATCCGCAAA 
TACAAAAAAC GCTTTAACTC CGAGATCTTG TGTGCTGCTC TATGGGGTGT TAACCTCTTG 
GTGGGCACTG AAAATGGTCT GATGCTGCTG GATCGTAGCG GTCAGGGCAA GGTTTATAAC 
CTCATCACCC GCAGACGCTT CCAACAGATG GATGTGCTGG AGGGTCTAAA TGTCCTGGTC 
ACCATATCAG GGAAAAAAAA TAAGCTCCGT GTGTACTACT TGTCATGGCT AAGAAACCGA 
ATACTGCACA ATGACCCGGA GGTGGAGAAG AAGCAGGGCT GGATCACTGT TGGTGAACTC 
GAAGGCTGCA TCCACTATAA AGTTGTGAAA TACGAGAGAA TCAAATTCCT GGTGATTGCA 
CTGAAGAACT CGGTAGAGAT TTACGCATGG GCCCCCAAGC CGTACCATAA ATTCATGGCT 
TTCAAGTCGT TCACAGAGCT GCAGCACAGA CCTCAGCTGG TGGACCTCAC AGTAGAAGAG 
GGTCAGAGGT TAAAGGTCAT CTACGGCTCT AGTGTGGGCT TTCACGTCAT CGATGTGGAT 
TCCGGAAGCC CCTACGACAT CTACATACCG TCACATATTC AGAGCACTGT GACTCCTCAT 
GCCATCGTGG TGCTTCCGAA GACTGATGGC ATGGAGATGC TGCTGTGTTA TGAAGATGAG 
GGCGTTTATG TCAATACCTA CGGCAGAATC ACCAAGGACG TTGTCTTGCA GTGGGGAGAG 
ATGCCCACCT CTGTTGCTTA TATTCATTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCTGTGGA AACTGGTCAC CTGGATGGGG TTTTCATGCA CAAGAGAGCT 
CAGAGACTCA AGTTCTTGTG TGAGAGGAAC GACAAGGTGT TCTTCGCCTC AGTGCGGTCA 
GGTGGCAGCA GTCAAGTGTT CTTCATGACC CTGAATAGGA ACTCTCTGAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163444.1
CDS	313..4572
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163387.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163387.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
TTGTCCGGTA CTACCCAGGT GCCCCAGAGG ACCACTTCTA TCTCCCCTGC TCTTGCTAGG 
AAAAACTCTC CTGGAAACGG AGAGTCCAGT GGAGGGGGGC GGTCGGCCTC TCAGCTCATT 
CGCACCAGTA ATCCAGACCT GAGGAGGACA GAGTTGTCTC TGGATGTGGC CCTGCAGAGA 
ACCAGCAGCA ACAGCTCCTC CAGCTCCAGC ACACCCAGCT CTCAGGGTGG ATCCCAGCCT 
GGATCCAGTG AGAGGAACCA GCCAAGAGGA TCTGGTAAAT CAGAGGGATC TCCAGTGGCA 
TTACAGGAAA CCTCCAAGCC CATTCAGGAG GAAGGCAGAG AGCTAAGCAA ACCCAGTCGG 
CCTGCAGATC TGACTGCCCT GGCTAAAGAA CTGAGGGAGC TGCGAATAGA TGAGGTCAGT 
CGACCCCCTC TTAAACTCAC AGACTACTCC TCTTCTAGTG AGGACTCTGA TAGCAGTGAG 
GATGAGGATG GTACGGTGGG ACACGACGGC ACTGTGTCAG TCAGTGACAT ACCCAGGATC 
ATGCCATCCG TTCAAGGAAA TAATGAGTCG TTCACGAGCA GCCATGATGA TTCCCTCACT 
GACTCCTACA ATGATGAGTC TAAAGATGGG ACACTGAGGA TGCGAGAGAA CAATGAGAGG 
CAACGCTTAG GCCATGGCGA GAGCAATGGC TTTGCAGGCC ATGGAAACCT ACCAGACCTC 
CTGCAGCAGA GCCACTCTCC ATCCGGAACT CCAACAGGCC TCAATTCCCA AGACCTGGAC 
TCTATGGATG AGTTTGGTCT TGGCGGTGGT TCGAAAGCTT CTTTCACTCC TTTTGTGGAC 
CCACGAGTGT ATCAAACCTC CCCCACAGAC GACAACGATG AGAGCTCAGC AGCCGCTATG 
TTTGCGAATG AGCTGTTGCG TCAAGAGCAG GCCAAACTGA ATGAGGCCCG CAAGATCTCT 
GTGGTGAACG TCAACCCCAC CAACATCAGA CCTCACAGCG ACACGCCAGA GATCCGCAAA 
TACAAAAAAC GCTTTAACTC CGAGATCTTG TGTGCTGCTC TATGGGGTGT TAACCTCTTG 
GTGGGCACTG AAAATGGTCT GATGCTGCTG GATCGTAGCG GTCAGGGCAA GGTTTATAAC 
CTCATCACCC GCAGACGCTT CCAACAGATG GATGTGCTGG AGGGTCTAAA TGTCCTGGTC 
ACCATATCAG GGAAAAAAAA TAAGCTCCGT GTGTACTACT TGTCATGGCT AAGAAACCGA 
ATACTGCACA ATGACCCGGA GGTGGAGAAG AAGCAGGGCT GGATCACTGT TGGTGAACTC 
GAAGGCTGCA TCCACTATAA AGTTGTGAAA TACGAGAGAA TCAAATTCCT GGTGATTGCA 
CTGAAGAACT CGGTAGAGAT TTACGCATGG GCCCCCAAGC CGTACCATAA ATTCATGGCT 
TTCAAGTCGT TCACAGAGCT GCAGCACAGA CCTCAGCTGG TGGACCTCAC AGTAGAAGAG 
GGTCAGAGGT TAAAGGTCAT CTACGGCTCT AGTGTGGGCT TTCACGTCAT CGATGTGGAT 
TCCGGAAGCC CCTACGACAT CTACATACCG TCACATATTC AGAGCACTGT GACTCCTCAT 
GCCATCGTGG TGCTTCCGAA GACTGATGGC ATGGAGATGC TGCTGTGTTA TGAAGATGAG 
GGCGTTTATG TCAATACCTA CGGCAGAATC ACCAAGGACG TTGTCTTGCA GTGGGGAGAG 
ATGCCCACCT CTGTTGCTTA TATTCATTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCTGTGGA AACTGGTCAC CTGGATGGGG TTTTCATGCA CAAGAGAGCT 
CAGAGACTCA AGTTCTTGTG TGAGAGGAAC GACAAGGTGT TCTTCGCCTC AGTGCGGTCA 
GGTGGCAGCA GTCAAGTGTT CTTCATGACC CTGAATAGGA ACTCTCTGAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163388.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163445.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07033
Clone ID Related Accession (Same CDS sequence)	XM_005163388.3 , XM_005163388.4
Accession Version	XM_005163388.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
GTGCCCCAGA GGACCACTTC TATCTCCCCT GCTCTTGCTA GGAAAAACTC TCCTGGAAAC 
GGAGAGTCCA GTGGAGGGGG GCGGTCGGCC TCTCAGCTCA TTCGCACCAG TAATCCAGAC 
CTGAGGAGGA CAGAGTTGTC TCTGGATGTG GCCCTGCAGA GAACCAGCAG CAACAGCTCC 
TCCAGCTCCA GCACACCCAG CTCTCAGGGT GGATCCCAGC CTGGATCCAG TGAGAGGAAC 
CAGCCAAGAG GATCTGGTAA ATCAGAGGGA TCTCCAGTGG CATTACAGGA AACCTCCAAG 
CCCATTCAGG AGGAAGGCAG AGAGCTAAGC AAACCCAGTC GGCCTGCAGA TCTGACTGCC 
CTGGCTAAAG AACTGAGGGA GCTGCGAATA GATGAGGTCA GTCGACCCCC TCTTAAACTC 
ACAGACTACT CCTCTTCTAG TGAGGACTCT GATAGCAGTG AGGATGAGGA TGGTACGGTG 
GGACACGACG GCACTGTGTC AGTCAGTGAC ATACCCAGGA TCATGCCATC CGTTCAAGGA 
AATAATGAGT CGTTCACGAG CAGCCATGAT GATTCCCTCA CTGACTCCTA CAATGATGAG 
TCTAAAGATG GGACACTGAG GATGCGAGAG AACAATGAGA GGCAACGCTT AGGCCATGGC 
GAGAGCAATG GCTTTGCAGG CCATGGAAAC CTACCAGACC TCCTGCAGCA GAGCCACTCT 
CCATCCGGAA CTCCAACAGG CCTCAATTCC CAAGACCTGG ACTCTATGGA TGAGTTTGGT 
CTTGGCGGTG GTTCGAAAGC TTCTTTCACT CCTTTTGTGG ACCCACGAGT GTATCAAACC 
TCCCCCACAG ACGACAACGA TGAGAGCTCA GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG 
CGTCAAGAGC AGGCCAAACT GAATGAGGCC CGCAAGATCT CTGTGGTGAA CGTCAACCCC 
ACCAACATCA GACCTCACAG CGACACGCCA GAGATCCGCA AATACAAAAA ACGCTTTAAC 
TCCGAGATCT TGTGTGCTGC TCTATGGGGT GTTAACCTCT TGGTGGGCAC TGAAAATGGT 
CTGATGCTGC TGGATCGTAG CGGTCAGGGC AAGGTTTATA ACCTCATCAC CCGCAGACGC 
TTCCAACAGA TGGATGTGCT GGAGGGTCTA AATGTCCTGG TCACCATATC AGGGAAAAAA 
AATAAGCTCC GTGTGTACTA CTTGTCATGG CTAAGAAACC GAATACTGCA CAATGACCCG 
GAGGTGGAGA AGAAGCAGGG CTGGATCACT GTTGGTGAAC TCGAAGGCTG CATCCACTAT 
AAAGTTGTGA AATACGAGAG AATCAAATTC CTGGTGATTG CACTGAAGAA CTCGGTAGAG 
ATTTACGCAT GGGCCCCCAA GCCGTACCAT AAATTCATGG CTTTCAAGTC GTTCACAGAG 
CTGCAGCACA GACCTCAGCT GGTGGACCTC ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTACGGCT CTAGTGTGGG CTTTCACGTC ATCGATGTGG ATTCCGGAAG CCCCTACGAC 
ATCTACATAC CGTCACATAT TCAGAGCACT GTGACTCCTC ATGCCATCGT GGTGCTTCCG 
AAGACTGATG GCATGGAGAT GCTGCTGTGT TATGAAGATG AGGGCGTTTA TGTCAATACC 
TACGGCAGAA TCACCAAGGA CGTTGTCTTG CAGTGGGGAG AGATGCCCAC CTCTGTTGCT 
TATATTCATT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCTGTG 
GAAACTGGTC ACCTGGATGG GGTTTTCATG CACAAGAGAG CTCAGAGACT CAAGTTCTTG 
TGTGAGAGGA ACGACAAGGT GTTCTTCGCC TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG 
TTCTTCATGA CCCTGAATAG GAACTCTCTG ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163445.1
CDS	312..4553
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163388.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163388.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
GTGCCCCAGA GGACCACTTC TATCTCCCCT GCTCTTGCTA GGAAAAACTC TCCTGGAAAC 
GGAGAGTCCA GTGGAGGGGG GCGGTCGGCC TCTCAGCTCA TTCGCACCAG TAATCCAGAC 
CTGAGGAGGA CAGAGTTGTC TCTGGATGTG GCCCTGCAGA GAACCAGCAG CAACAGCTCC 
TCCAGCTCCA GCACACCCAG CTCTCAGGGT GGATCCCAGC CTGGATCCAG TGAGAGGAAC 
CAGCCAAGAG GATCTGGTAA ATCAGAGGGA TCTCCAGTGG CATTACAGGA AACCTCCAAG 
CCCATTCAGG AGGAAGGCAG AGAGCTAAGC AAACCCAGTC GGCCTGCAGA TCTGACTGCC 
CTGGCTAAAG AACTGAGGGA GCTGCGAATA GATGAGGTCA GTCGACCCCC TCTTAAACTC 
ACAGACTACT CCTCTTCTAG TGAGGACTCT GATAGCAGTG AGGATGAGGA TGGTACGGTG 
GGACACGACG GCACTGTGTC AGTCAGTGAC ATACCCAGGA TCATGCCATC CGTTCAAGGA 
AATAATGAGT CGTTCACGAG CAGCCATGAT GATTCCCTCA CTGACTCCTA CAATGATGAG 
TCTAAAGATG GGACACTGAG GATGCGAGAG AACAATGAGA GGCAACGCTT AGGCCATGGC 
GAGAGCAATG GCTTTGCAGG CCATGGAAAC CTACCAGACC TCCTGCAGCA GAGCCACTCT 
CCATCCGGAA CTCCAACAGG CCTCAATTCC CAAGACCTGG ACTCTATGGA TGAGTTTGGT 
CTTGGCGGTG GTTCGAAAGC TTCTTTCACT CCTTTTGTGG ACCCACGAGT GTATCAAACC 
TCCCCCACAG ACGACAACGA TGAGAGCTCA GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG 
CGTCAAGAGC AGGCCAAACT GAATGAGGCC CGCAAGATCT CTGTGGTGAA CGTCAACCCC 
ACCAACATCA GACCTCACAG CGACACGCCA GAGATCCGCA AATACAAAAA ACGCTTTAAC 
TCCGAGATCT TGTGTGCTGC TCTATGGGGT GTTAACCTCT TGGTGGGCAC TGAAAATGGT 
CTGATGCTGC TGGATCGTAG CGGTCAGGGC AAGGTTTATA ACCTCATCAC CCGCAGACGC 
TTCCAACAGA TGGATGTGCT GGAGGGTCTA AATGTCCTGG TCACCATATC AGGGAAAAAA 
AATAAGCTCC GTGTGTACTA CTTGTCATGG CTAAGAAACC GAATACTGCA CAATGACCCG 
GAGGTGGAGA AGAAGCAGGG CTGGATCACT GTTGGTGAAC TCGAAGGCTG CATCCACTAT 
AAAGTTGTGA AATACGAGAG AATCAAATTC CTGGTGATTG CACTGAAGAA CTCGGTAGAG 
ATTTACGCAT GGGCCCCCAA GCCGTACCAT AAATTCATGG CTTTCAAGTC GTTCACAGAG 
CTGCAGCACA GACCTCAGCT GGTGGACCTC ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTACGGCT CTAGTGTGGG CTTTCACGTC ATCGATGTGG ATTCCGGAAG CCCCTACGAC 
ATCTACATAC CGTCACATAT TCAGAGCACT GTGACTCCTC ATGCCATCGT GGTGCTTCCG 
AAGACTGATG GCATGGAGAT GCTGCTGTGT TATGAAGATG AGGGCGTTTA TGTCAATACC 
TACGGCAGAA TCACCAAGGA CGTTGTCTTG CAGTGGGGAG AGATGCCCAC CTCTGTTGCT 
TATATTCATT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCTGTG 
GAAACTGGTC ACCTGGATGG GGTTTTCATG CACAAGAGAG CTCAGAGACT CAAGTTCTTG 
TGTGAGAGGA ACGACAAGGT GTTCTTCGCC TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG 
TTCTTCATGA CCCTGAATAG GAACTCTCTG ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163388.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163445.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.

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CloneID	ODa07033
Clone ID Related Accession (Same CDS sequence)	XM_005163388.3 , XM_005163388.4
Accession Version	XM_005163388.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163388.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
GTGCCCCAGA GGACCACTTC TATCTCCCCT GCTCTTGCTA GGAAAAACTC TCCTGGAAAC 
GGAGAGTCCA GTGGAGGGGG GCGGTCGGCC TCTCAGCTCA TTCGCACCAG TAATCCAGAC 
CTGAGGAGGA CAGAGTTGTC TCTGGATGTG GCCCTGCAGA GAACCAGCAG CAACAGCTCC 
TCCAGCTCCA GCACACCCAG CTCTCAGGGT GGATCCCAGC CTGGATCCAG TGAGAGGAAC 
CAGCCAAGAG GATCTGGTAA ATCAGAGGGA TCTCCAGTGG CATTACAGGA AACCTCCAAG 
CCCATTCAGG AGGAAGGCAG AGAGCTAAGC AAACCCAGTC GGCCTGCAGA TCTGACTGCC 
CTGGCTAAAG AACTGAGGGA GCTGCGAATA GATGAGGTCA GTCGACCCCC TCTTAAACTC 
ACAGACTACT CCTCTTCTAG TGAGGACTCT GATAGCAGTG AGGATGAGGA TGGTACGGTG 
GGACACGACG GCACTGTGTC AGTCAGTGAC ATACCCAGGA TCATGCCATC CGTTCAAGGA 
AATAATGAGT CGTTCACGAG CAGCCATGAT GATTCCCTCA CTGACTCCTA CAATGATGAG 
TCTAAAGATG GGACACTGAG GATGCGAGAG AACAATGAGA GGCAACGCTT AGGCCATGGC 
GAGAGCAATG GCTTTGCAGG CCATGGAAAC CTACCAGACC TCCTGCAGCA GAGCCACTCT 
CCATCCGGAA CTCCAACAGG CCTCAATTCC CAAGACCTGG ACTCTATGGA TGAGTTTGGT 
CTTGGCGGTG GTTCGAAAGC TTCTTTCACT CCTTTTGTGG ACCCACGAGT GTATCAAACC 
TCCCCCACAG ACGACAACGA TGAGAGCTCA GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG 
CGTCAAGAGC AGGCCAAACT GAATGAGGCC CGCAAGATCT CTGTGGTGAA CGTCAACCCC 
ACCAACATCA GACCTCACAG CGACACGCCA GAGATCCGCA AATACAAAAA ACGCTTTAAC 
TCCGAGATCT TGTGTGCTGC TCTATGGGGT GTTAACCTCT TGGTGGGCAC TGAAAATGGT 
CTGATGCTGC TGGATCGTAG CGGTCAGGGC AAGGTTTATA ACCTCATCAC CCGCAGACGC 
TTCCAACAGA TGGATGTGCT GGAGGGTCTA AATGTCCTGG TCACCATATC AGGGAAAAAA 
AATAAGCTCC GTGTGTACTA CTTGTCATGG CTAAGAAACC GAATACTGCA CAATGACCCG 
GAGGTGGAGA AGAAGCAGGG CTGGATCACT GTTGGTGAAC TCGAAGGCTG CATCCACTAT 
AAAGTTGTGA AATACGAGAG AATCAAATTC CTGGTGATTG CACTGAAGAA CTCGGTAGAG 
ATTTACGCAT GGGCCCCCAA GCCGTACCAT AAATTCATGG CTTTCAAGTC GTTCACAGAG 
CTGCAGCACA GACCTCAGCT GGTGGACCTC ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTACGGCT CTAGTGTGGG CTTTCACGTC ATCGATGTGG ATTCCGGAAG CCCCTACGAC 
ATCTACATAC CGTCACATAT TCAGAGCACT GTGACTCCTC ATGCCATCGT GGTGCTTCCG 
AAGACTGATG GCATGGAGAT GCTGCTGTGT TATGAAGATG AGGGCGTTTA TGTCAATACC 
TACGGCAGAA TCACCAAGGA CGTTGTCTTG CAGTGGGGAG AGATGCCCAC CTCTGTTGCT 
TATATTCATT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCTGTG 
GAAACTGGTC ACCTGGATGG GGTTTTCATG CACAAGAGAG CTCAGAGACT CAAGTTCTTG 
TGTGAGAGGA ACGACAAGGT GTTCTTCGCC TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG 
TTCTTCATGA CCCTGAATAG GAACTCTCTG ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163445.1
CDS	313..4554
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYIRRQLEEEQRQLEILQQQLLQEQALLLEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163388.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163388.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATATCC GTCGTCAGTT GGAGGAGGAA CAGAGACAAC TCGAGATCCT ACAACAACAG 
CTCTTGCAAG AGCAGGCGCT CCTACTGGAG TATAAACGTA AACAGGTGGA GGAACAAAGA 
CAGGCGGATA GATTGCAGAG GCAGCTCCAG CAAGAAAGAG CTTACCTCGT CTCTCTCCAA 
CAACAGCAAC AGCAGCAACA ACAAGACTCC AGACCTGAAG AGAAGAAGCA ACTTTACCAT 
TACAAAGATG CTGTCAACCC CAGCGACAAA CCAGCGTGGG CTAAAAAGAT CCATGGACCG 
CCCCAGATAA AGATCCAACC CAGCAACCGC AAGCAGTCCA CACCCCTCTA CTATAAGTTG 
GACAACCTAG GGATCCCCAT AGGCCGCGCT ATACTGAATC CCTCAATTAA TCAAAGTGTC 
ATGCAGCCTT TAAAGCCGCA GAAGCAGAAC CTGATACAGC GACAATCGAT TTCACCACCC 
CCCCAATACA GTGCAAAAAA AATTAGTATG GGACCTCGCC CAGGCTTTTC CCGTCCTCCT 
TATAAGCGGG TTGAGGAGCG ATCTAAACTG AACCGCCAGA GCTCTCCTGC CCTACAGCAT 
AAAGTGGCCA ATCGTATCTC TGACCCTTCC CTCCCGCCTC GCTCTGAGTC CTTCAGCAGT 
GGAGGAATGC AGCCAGCACG CACCCCTCCT ATGCACCGCC CTGTGGAGCC CCAGATGGCT 
CATTTGGTTC CAGTGAGGTC CCATAGCTCC TCTATGTCAG TCTCGCAGTC TTTGCATGAT 
CAATCTGCAA AGGGTGTTTC AGCCTTCCAG GAAAGCCTGG TGTCCAATCG ACCAGAGATG 
CCTCGACAGA ACTCCGACCC GACTTCAGAG ATGCCCCCTC CTGCCCCTCG CATGGCCAGC 
CATGAGGAAA AACCTGACCG CAGTTCCCCC TGGCTGAGGG AAGACGCCGT TCCTCCCAAG 
GTGCCCCAGA GGACCACTTC TATCTCCCCT GCTCTTGCTA GGAAAAACTC TCCTGGAAAC 
GGAGAGTCCA GTGGAGGGGG GCGGTCGGCC TCTCAGCTCA TTCGCACCAG TAATCCAGAC 
CTGAGGAGGA CAGAGTTGTC TCTGGATGTG GCCCTGCAGA GAACCAGCAG CAACAGCTCC 
TCCAGCTCCA GCACACCCAG CTCTCAGGGT GGATCCCAGC CTGGATCCAG TGAGAGGAAC 
CAGCCAAGAG GATCTGGTAA ATCAGAGGGA TCTCCAGTGG CATTACAGGA AACCTCCAAG 
CCCATTCAGG AGGAAGGCAG AGAGCTAAGC AAACCCAGTC GGCCTGCAGA TCTGACTGCC 
CTGGCTAAAG AACTGAGGGA GCTGCGAATA GATGAGGTCA GTCGACCCCC TCTTAAACTC 
ACAGACTACT CCTCTTCTAG TGAGGACTCT GATAGCAGTG AGGATGAGGA TGGTACGGTG 
GGACACGACG GCACTGTGTC AGTCAGTGAC ATACCCAGGA TCATGCCATC CGTTCAAGGA 
AATAATGAGT CGTTCACGAG CAGCCATGAT GATTCCCTCA CTGACTCCTA CAATGATGAG 
TCTAAAGATG GGACACTGAG GATGCGAGAG AACAATGAGA GGCAACGCTT AGGCCATGGC 
GAGAGCAATG GCTTTGCAGG CCATGGAAAC CTACCAGACC TCCTGCAGCA GAGCCACTCT 
CCATCCGGAA CTCCAACAGG CCTCAATTCC CAAGACCTGG ACTCTATGGA TGAGTTTGGT 
CTTGGCGGTG GTTCGAAAGC TTCTTTCACT CCTTTTGTGG ACCCACGAGT GTATCAAACC 
TCCCCCACAG ACGACAACGA TGAGAGCTCA GCAGCCGCTA TGTTTGCGAA TGAGCTGTTG 
CGTCAAGAGC AGGCCAAACT GAATGAGGCC CGCAAGATCT CTGTGGTGAA CGTCAACCCC 
ACCAACATCA GACCTCACAG CGACACGCCA GAGATCCGCA AATACAAAAA ACGCTTTAAC 
TCCGAGATCT TGTGTGCTGC TCTATGGGGT GTTAACCTCT TGGTGGGCAC TGAAAATGGT 
CTGATGCTGC TGGATCGTAG CGGTCAGGGC AAGGTTTATA ACCTCATCAC CCGCAGACGC 
TTCCAACAGA TGGATGTGCT GGAGGGTCTA AATGTCCTGG TCACCATATC AGGGAAAAAA 
AATAAGCTCC GTGTGTACTA CTTGTCATGG CTAAGAAACC GAATACTGCA CAATGACCCG 
GAGGTGGAGA AGAAGCAGGG CTGGATCACT GTTGGTGAAC TCGAAGGCTG CATCCACTAT 
AAAGTTGTGA AATACGAGAG AATCAAATTC CTGGTGATTG CACTGAAGAA CTCGGTAGAG 
ATTTACGCAT GGGCCCCCAA GCCGTACCAT AAATTCATGG CTTTCAAGTC GTTCACAGAG 
CTGCAGCACA GACCTCAGCT GGTGGACCTC ACAGTAGAAG AGGGTCAGAG GTTAAAGGTC 
ATCTACGGCT CTAGTGTGGG CTTTCACGTC ATCGATGTGG ATTCCGGAAG CCCCTACGAC 
ATCTACATAC CGTCACATAT TCAGAGCACT GTGACTCCTC ATGCCATCGT GGTGCTTCCG 
AAGACTGATG GCATGGAGAT GCTGCTGTGT TATGAAGATG AGGGCGTTTA TGTCAATACC 
TACGGCAGAA TCACCAAGGA CGTTGTCTTG CAGTGGGGAG AGATGCCCAC CTCTGTTGCT 
TATATTCATT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCTGTG 
GAAACTGGTC ACCTGGATGG GGTTTTCATG CACAAGAGAG CTCAGAGACT CAAGTTCTTG 
TGTGAGAGGA ACGACAAGGT GTTCTTCGCC TCAGTGCGGT CAGGTGGCAG CAGTCAAGTG 
TTCTTCATGA CCCTGAATAG GAACTCTCTG ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163389.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163446.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.

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CloneID	ODa07034
Clone ID Related Accession (Same CDS sequence)	XM_005163389.3 , XM_005163389.4
Accession Version	XM_005163389.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GATCCATGGA CCGCCCCAGA TAAAGATCCA ACCCAGCAAC 
CGCAAGCAGT CCACACCCCT CTACTATAAG TTGGACAACC TAGGGATCCC CATAGGCCGC 
GCTATACTGA ATCCCTCAAT TAATCAAAGT GTCATGCAGC CTTTAAAGCC GCAGAAGCAG 
AACCTGATAC AGCGACAATC GATTTCACCA CCCCCCCAAT ACAGTGCAAA AAAAATTAGT 
ATGGGACCTC GCCCAGGCTT TTCCCGTCCT CCTTATAAGC GGGTTGAGGA GCGATCTAAA 
CTGAACCGCC AGAGCTCTCC TGCCCTACAG CATAAAGTGG CCAATCGTAT CTCTGACCCT 
TCCCTCCCGC CTCGCTCTGA GTCCTTCAGC AGTGGAGGAA TGCAGCCAGC ACGCACCCCT 
CCTATGCACC GCCCTGTGGA GCCCCAGATG GCTCATTTGG TTCCAGTGAG GTCCCATAGC 
TCCTCTATGT CAGTCTCGCA GTCTTTGCAT GATCAATCTG CAAAGGGTGT TTCAGCCTTC 
CAGGAAAGCC TGGTGTCCAA TCGACCAGAG ATGCCTCGAC AGAACTCCGA CCCGACTTCA 
GAGATGCCCC CTCCTGCCCC TCGCATGGCC AGCCATGAGG AAAAACCTGA CCGCAGTTCC 
CCCTGGCTGA GGGAAGACGC CGTTCCTCCC AAGTTGTCCG GTACTACCCA GGTGCCCCAG 
AGGACCACTT CTATCTCCCC TGCTCTTGCT AGGAAAAACT CTCCTGGAAA CGGAGAGTCC 
AGTGGAGGGG GGCGGTCGGC CTCTCAGCTC ATTCGCACCA GTAATCCAGA CCTGAGGAGG 
ACAGAGTTGT CTCTGGATGT GGCCCTGCAG AGAACCAGCA GCAACAGCTC CTCCAGCTCC 
AGCACACCCA GCTCTCAGGG TGGATCCCAG CCTGGATCCA GTGAGAGGAA CCAGCCAAGA 
GGATCTGGTA AATCAGAGGG ATCTCCAGTG GCATTACAGG AAACCTCCAA GCCCATTCAG 
GAGGAAGGCA GAGAGCTAAG CAAACCCAGT CGGCCTGCAG ATCTGACTGC CCTGGCTAAA 
GAACTGAGGG AGCTGCGAAT AGATGAGGTC AGTCGACCCC CTCTTAAACT CACAGACTAC 
TCCTCTTCTA GTGAGGACTC TGATAGCAGT GAGGATGAGG ATGGTACGGT GGGACACGAC 
GGCACTGTGT CAGTCAGTGA CATACCCAGG ATCATGCCAT CCGTTCAAGG AAATAATGAG 
TCGTTCACGA GCAGCCATGA TGATTCCCTC ACTGACTCCT ACAATGATGA GTCTAAAGAT 
GGGACACTGA GGATGCGAGA GAACAATGAG AGGCAACGCT TAGGCCATGG CGAGAGCAAT 
GGCTTTGCAG GCCATGGAAA CCTACCAGAC CTCCTGCAGC AGAGCCACTC TCCATCCGGA 
ACTCCAACAG GCCTCAATTC CCAAGACCTG GACTCTATGG ATGAGTTTGG TCTTGGCGGT 
GGTTCGAAAG CTTCTTTCAC TCCTTTTGTG GACCCACGAG TGTATCAAAC CTCCCCCACA 
GACGACAACG ATGAGAGCTC AGCAGCCGCT ATGTTTGCGA ATGAGCTGTT GCGTCAAGAG 
CAGGCCAAAC TGAATGAGGC CCGCAAGATC TCTGTGGTGA ACGTCAACCC CACCAACATC 
AGACCTCACA GCGACACGCC AGAGATCCGC AAATACAAAA AACGCTTTAA CTCCGAGATC 
TTGTGTGCTG CTCTATGGGG TGTTAACCTC TTGGTGGGCA CTGAAAATGG TCTGATGCTG 
CTGGATCGTA GCGGTCAGGG CAAGGTTTAT AACCTCATCA CCCGCAGACG CTTCCAACAG 
ATGGATGTGC TGGAGGGTCT AAATGTCCTG GTCACCATAT CAGGGAAAAA AAATAAGCTC 
CGTGTGTACT ACTTGTCATG GCTAAGAAAC CGAATACTGC ACAATGACCC GGAGGTGGAG 
AAGAAGCAGG GCTGGATCAC TGTTGGTGAA CTCGAAGGCT GCATCCACTA TAAAGTTGTG 
AAATACGAGA GAATCAAATT CCTGGTGATT GCACTGAAGA ACTCGGTAGA GATTTACGCA 
TGGGCCCCCA AGCCGTACCA TAAATTCATG GCTTTCAAGT CGTTCACAGA GCTGCAGCAC 
AGACCTCAGC TGGTGGACCT CACAGTAGAA GAGGGTCAGA GGTTAAAGGT CATCTACGGC 
TCTAGTGTGG GCTTTCACGT CATCGATGTG GATTCCGGAA GCCCCTACGA CATCTACATA 
CCGTCACATA TTCAGAGCAC TGTGACTCCT CATGCCATCG TGGTGCTTCC GAAGACTGAT 
GGCATGGAGA TGCTGCTGTG TTATGAAGAT GAGGGCGTTT ATGTCAATAC CTACGGCAGA 
ATCACCAAGG ACGTTGTCTT GCAGTGGGGA GAGATGCCCA CCTCTGTTGC TTATATTCAT 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCTGT GGAAACTGGT 
CACCTGGATG GGGTTTTCAT GCACAAGAGA GCTCAGAGAC TCAAGTTCTT GTGTGAGAGG 
AACGACAAGG TGTTCTTCGC CTCAGTGCGG TCAGGTGGCA GCAGTCAAGT GTTCTTCATG 
ACCCTGAATA GGAACTCTCT GATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163446.1
CDS	312..4484
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163389.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163389.3

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GATCCATGGA CCGCCCCAGA TAAAGATCCA ACCCAGCAAC 
CGCAAGCAGT CCACACCCCT CTACTATAAG TTGGACAACC TAGGGATCCC CATAGGCCGC 
GCTATACTGA ATCCCTCAAT TAATCAAAGT GTCATGCAGC CTTTAAAGCC GCAGAAGCAG 
AACCTGATAC AGCGACAATC GATTTCACCA CCCCCCCAAT ACAGTGCAAA AAAAATTAGT 
ATGGGACCTC GCCCAGGCTT TTCCCGTCCT CCTTATAAGC GGGTTGAGGA GCGATCTAAA 
CTGAACCGCC AGAGCTCTCC TGCCCTACAG CATAAAGTGG CCAATCGTAT CTCTGACCCT 
TCCCTCCCGC CTCGCTCTGA GTCCTTCAGC AGTGGAGGAA TGCAGCCAGC ACGCACCCCT 
CCTATGCACC GCCCTGTGGA GCCCCAGATG GCTCATTTGG TTCCAGTGAG GTCCCATAGC 
TCCTCTATGT CAGTCTCGCA GTCTTTGCAT GATCAATCTG CAAAGGGTGT TTCAGCCTTC 
CAGGAAAGCC TGGTGTCCAA TCGACCAGAG ATGCCTCGAC AGAACTCCGA CCCGACTTCA 
GAGATGCCCC CTCCTGCCCC TCGCATGGCC AGCCATGAGG AAAAACCTGA CCGCAGTTCC 
CCCTGGCTGA GGGAAGACGC CGTTCCTCCC AAGTTGTCCG GTACTACCCA GGTGCCCCAG 
AGGACCACTT CTATCTCCCC TGCTCTTGCT AGGAAAAACT CTCCTGGAAA CGGAGAGTCC 
AGTGGAGGGG GGCGGTCGGC CTCTCAGCTC ATTCGCACCA GTAATCCAGA CCTGAGGAGG 
ACAGAGTTGT CTCTGGATGT GGCCCTGCAG AGAACCAGCA GCAACAGCTC CTCCAGCTCC 
AGCACACCCA GCTCTCAGGG TGGATCCCAG CCTGGATCCA GTGAGAGGAA CCAGCCAAGA 
GGATCTGGTA AATCAGAGGG ATCTCCAGTG GCATTACAGG AAACCTCCAA GCCCATTCAG 
GAGGAAGGCA GAGAGCTAAG CAAACCCAGT CGGCCTGCAG ATCTGACTGC CCTGGCTAAA 
GAACTGAGGG AGCTGCGAAT AGATGAGGTC AGTCGACCCC CTCTTAAACT CACAGACTAC 
TCCTCTTCTA GTGAGGACTC TGATAGCAGT GAGGATGAGG ATGGTACGGT GGGACACGAC 
GGCACTGTGT CAGTCAGTGA CATACCCAGG ATCATGCCAT CCGTTCAAGG AAATAATGAG 
TCGTTCACGA GCAGCCATGA TGATTCCCTC ACTGACTCCT ACAATGATGA GTCTAAAGAT 
GGGACACTGA GGATGCGAGA GAACAATGAG AGGCAACGCT TAGGCCATGG CGAGAGCAAT 
GGCTTTGCAG GCCATGGAAA CCTACCAGAC CTCCTGCAGC AGAGCCACTC TCCATCCGGA 
ACTCCAACAG GCCTCAATTC CCAAGACCTG GACTCTATGG ATGAGTTTGG TCTTGGCGGT 
GGTTCGAAAG CTTCTTTCAC TCCTTTTGTG GACCCACGAG TGTATCAAAC CTCCCCCACA 
GACGACAACG ATGAGAGCTC AGCAGCCGCT ATGTTTGCGA ATGAGCTGTT GCGTCAAGAG 
CAGGCCAAAC TGAATGAGGC CCGCAAGATC TCTGTGGTGA ACGTCAACCC CACCAACATC 
AGACCTCACA GCGACACGCC AGAGATCCGC AAATACAAAA AACGCTTTAA CTCCGAGATC 
TTGTGTGCTG CTCTATGGGG TGTTAACCTC TTGGTGGGCA CTGAAAATGG TCTGATGCTG 
CTGGATCGTA GCGGTCAGGG CAAGGTTTAT AACCTCATCA CCCGCAGACG CTTCCAACAG 
ATGGATGTGC TGGAGGGTCT AAATGTCCTG GTCACCATAT CAGGGAAAAA AAATAAGCTC 
CGTGTGTACT ACTTGTCATG GCTAAGAAAC CGAATACTGC ACAATGACCC GGAGGTGGAG 
AAGAAGCAGG GCTGGATCAC TGTTGGTGAA CTCGAAGGCT GCATCCACTA TAAAGTTGTG 
AAATACGAGA GAATCAAATT CCTGGTGATT GCACTGAAGA ACTCGGTAGA GATTTACGCA 
TGGGCCCCCA AGCCGTACCA TAAATTCATG GCTTTCAAGT CGTTCACAGA GCTGCAGCAC 
AGACCTCAGC TGGTGGACCT CACAGTAGAA GAGGGTCAGA GGTTAAAGGT CATCTACGGC 
TCTAGTGTGG GCTTTCACGT CATCGATGTG GATTCCGGAA GCCCCTACGA CATCTACATA 
CCGTCACATA TTCAGAGCAC TGTGACTCCT CATGCCATCG TGGTGCTTCC GAAGACTGAT 
GGCATGGAGA TGCTGCTGTG TTATGAAGAT GAGGGCGTTT ATGTCAATAC CTACGGCAGA 
ATCACCAAGG ACGTTGTCTT GCAGTGGGGA GAGATGCCCA CCTCTGTTGC TTATATTCAT 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCTGT GGAAACTGGT 
CACCTGGATG GGGTTTTCAT GCACAAGAGA GCTCAGAGAC TCAAGTTCTT GTGTGAGAGG 
AACGACAAGG TGTTCTTCGC CTCAGTGCGG TCAGGTGGCA GCAGTCAAGT GTTCTTCATG 
ACCCTGAATA GGAACTCTCT GATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnika ( XM_005163389.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163446.1 Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.

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CloneID	ODa07034
Clone ID Related Accession (Same CDS sequence)	XM_005163389.3 , XM_005163389.4
Accession Version	XM_005163389.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRAF2 and NCK interacting kinase a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163389.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GATCCATGGA CCGCCCCAGA TAAAGATCCA ACCCAGCAAC 
CGCAAGCAGT CCACACCCCT CTACTATAAG TTGGACAACC TAGGGATCCC CATAGGCCGC 
GCTATACTGA ATCCCTCAAT TAATCAAAGT GTCATGCAGC CTTTAAAGCC GCAGAAGCAG 
AACCTGATAC AGCGACAATC GATTTCACCA CCCCCCCAAT ACAGTGCAAA AAAAATTAGT 
ATGGGACCTC GCCCAGGCTT TTCCCGTCCT CCTTATAAGC GGGTTGAGGA GCGATCTAAA 
CTGAACCGCC AGAGCTCTCC TGCCCTACAG CATAAAGTGG CCAATCGTAT CTCTGACCCT 
TCCCTCCCGC CTCGCTCTGA GTCCTTCAGC AGTGGAGGAA TGCAGCCAGC ACGCACCCCT 
CCTATGCACC GCCCTGTGGA GCCCCAGATG GCTCATTTGG TTCCAGTGAG GTCCCATAGC 
TCCTCTATGT CAGTCTCGCA GTCTTTGCAT GATCAATCTG CAAAGGGTGT TTCAGCCTTC 
CAGGAAAGCC TGGTGTCCAA TCGACCAGAG ATGCCTCGAC AGAACTCCGA CCCGACTTCA 
GAGATGCCCC CTCCTGCCCC TCGCATGGCC AGCCATGAGG AAAAACCTGA CCGCAGTTCC 
CCCTGGCTGA GGGAAGACGC CGTTCCTCCC AAGTTGTCCG GTACTACCCA GGTGCCCCAG 
AGGACCACTT CTATCTCCCC TGCTCTTGCT AGGAAAAACT CTCCTGGAAA CGGAGAGTCC 
AGTGGAGGGG GGCGGTCGGC CTCTCAGCTC ATTCGCACCA GTAATCCAGA CCTGAGGAGG 
ACAGAGTTGT CTCTGGATGT GGCCCTGCAG AGAACCAGCA GCAACAGCTC CTCCAGCTCC 
AGCACACCCA GCTCTCAGGG TGGATCCCAG CCTGGATCCA GTGAGAGGAA CCAGCCAAGA 
GGATCTGGTA AATCAGAGGG ATCTCCAGTG GCATTACAGG AAACCTCCAA GCCCATTCAG 
GAGGAAGGCA GAGAGCTAAG CAAACCCAGT CGGCCTGCAG ATCTGACTGC CCTGGCTAAA 
GAACTGAGGG AGCTGCGAAT AGATGAGGTC AGTCGACCCC CTCTTAAACT CACAGACTAC 
TCCTCTTCTA GTGAGGACTC TGATAGCAGT GAGGATGAGG ATGGTACGGT GGGACACGAC 
GGCACTGTGT CAGTCAGTGA CATACCCAGG ATCATGCCAT CCGTTCAAGG AAATAATGAG 
TCGTTCACGA GCAGCCATGA TGATTCCCTC ACTGACTCCT ACAATGATGA GTCTAAAGAT 
GGGACACTGA GGATGCGAGA GAACAATGAG AGGCAACGCT TAGGCCATGG CGAGAGCAAT 
GGCTTTGCAG GCCATGGAAA CCTACCAGAC CTCCTGCAGC AGAGCCACTC TCCATCCGGA 
ACTCCAACAG GCCTCAATTC CCAAGACCTG GACTCTATGG ATGAGTTTGG TCTTGGCGGT 
GGTTCGAAAG CTTCTTTCAC TCCTTTTGTG GACCCACGAG TGTATCAAAC CTCCCCCACA 
GACGACAACG ATGAGAGCTC AGCAGCCGCT ATGTTTGCGA ATGAGCTGTT GCGTCAAGAG 
CAGGCCAAAC TGAATGAGGC CCGCAAGATC TCTGTGGTGA ACGTCAACCC CACCAACATC 
AGACCTCACA GCGACACGCC AGAGATCCGC AAATACAAAA AACGCTTTAA CTCCGAGATC 
TTGTGTGCTG CTCTATGGGG TGTTAACCTC TTGGTGGGCA CTGAAAATGG TCTGATGCTG 
CTGGATCGTA GCGGTCAGGG CAAGGTTTAT AACCTCATCA CCCGCAGACG CTTCCAACAG 
ATGGATGTGC TGGAGGGTCT AAATGTCCTG GTCACCATAT CAGGGAAAAA AAATAAGCTC 
CGTGTGTACT ACTTGTCATG GCTAAGAAAC CGAATACTGC ACAATGACCC GGAGGTGGAG 
AAGAAGCAGG GCTGGATCAC TGTTGGTGAA CTCGAAGGCT GCATCCACTA TAAAGTTGTG 
AAATACGAGA GAATCAAATT CCTGGTGATT GCACTGAAGA ACTCGGTAGA GATTTACGCA 
TGGGCCCCCA AGCCGTACCA TAAATTCATG GCTTTCAAGT CGTTCACAGA GCTGCAGCAC 
AGACCTCAGC TGGTGGACCT CACAGTAGAA GAGGGTCAGA GGTTAAAGGT CATCTACGGC 
TCTAGTGTGG GCTTTCACGT CATCGATGTG GATTCCGGAA GCCCCTACGA CATCTACATA 
CCGTCACATA TTCAGAGCAC TGTGACTCCT CATGCCATCG TGGTGCTTCC GAAGACTGAT 
GGCATGGAGA TGCTGCTGTG TTATGAAGAT GAGGGCGTTT ATGTCAATAC CTACGGCAGA 
ATCACCAAGG ACGTTGTCTT GCAGTGGGGA GAGATGCCCA CCTCTGTTGC TTATATTCAT 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCTGT GGAAACTGGT 
CACCTGGATG GGGTTTTCAT GCACAAGAGA GCTCAGAGAC TCAAGTTCTT GTGTGAGAGG 
AACGACAAGG TGTTCTTCGC CTCAGTGCGG TCAGGTGGCA GCAGTCAAGT GTTCTTCATG 
ACCCTGAATA GGAACTCTCT GATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163446.1
CDS	313..4485
Translation	MASDSPARSLDEIDLSTLRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKCMDVTGEEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGVDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEDWTAYISREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVSRRNTFIGTPYWMAPEVIACDENPDATYDYKSDLWSLGITALEMAEGAPPLCELHPMRALFLIPRNAPPRLKSKKWTKRFHSFIESCLVKNHNMRPGTDQLLKHPFIRDMANERTIRIQLKDHIDRTRKKRGERDETEYEYSGSEEEEEAMLEIGEPSSIINIPGESTLRRDFLRLQVANKERSDALRRQQLEQQQNEGHKHQLLAERQKRIEEQKEQRRRLEEQQRREREARKQQEQRHRYEELRREEERRQAEREQEYKRKQVEEQRQADRLQRQLQQERAYLVSLQQQQQQQQQDSRPEEKKQLYHYKDAVNPSDKPAWAKKIHGPPQIKIQPSNRKQSTPLYYKLDNLGIPIGRAILNPSINQSVMQPLKPQKQNLIQRQSISPPPQYSAKKISMGPRPGFSRPPYKRVEERSKLNRQSSPALQHKVANRISDPSLPPRSESFSSGGMQPARTPPMHRPVEPQMAHLVPVRSHSSSMSVSQSLHDQSAKGVSAFQESLVSNRPEMPRQNSDPTSEMPPPAPRMASHEEKPDRSSPWLREDAVPPKLSGTTQVPQRTTSISPALARKNSPGNGESSGGGRSASQLIRTSNPDLRRTELSLDVALQRTSSNSSSSSSTPSSQGGSQPGSSERNQPRGSGKSEGSPVALQETSKPIQEEGRELSKPSRPADLTALAKELRELRIDEVSRPPLKLTDYSSSSEDSDSSEDEDGTVGHDGTVSVSDIPRIMPSVQGNNESFTSSHDDSLTDSYNDESKDGTLRMRENNERQRLGHGESNGFAGHGNLPDLLQQSHSPSGTPTGLNSQDLDSMDEFGLGGGSKASFTPFVDPRVYQTSPTDDNDESSAAAMFANELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLITRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGELEGCIHYKVVKYERIKFLVIALKNSVEIYAWAPKPYHKFMAFKSFTELQHRPQLVDLTVEEGQRLKVIYGSSVGFHVIDVDSGSPYDIYIPSHIQSTVTPHAIVVLPKTDGMEMLLCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSLMNW

Target ORF information:

RefSeq Version	XM_005163389.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TRAF2 and NCK interacting kinase a (tnika), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163389.4

ORF Insert Sequence:

ATGGCGAGCG ATTCCCCGGC TCGGAGTCTG GATGAAATAG ACCTCTCCAC TTTAAGGGAC 
CCTGCAGGAA TTTTTGAGCT GGTGGAGCTG GTGGGCAATG GCACGTATGG TCAGGTGTAT 
AAGGGCCGAC ATGTCAAAAC AGGTCAACTG GCTGCTATCA AGTGCATGGA TGTCACAGGG 
GAGGAAGAGG AGGAGATCAA AGCGGAGATC AACATGTTGA AGAAATACTC TCACCACAGG 
AACATCGCCA CCTACTATGG TGCCTTTATT AAAAAGAACC CACCCGGCGT GGACGACCAG 
CTGTGGCTGG TGATGGAGTT TTGTGGCGCA GGCTCAGTGA CGGACCTGAT CAAGAACACC 
AAAGGAAACT CGTTGAAGGA AGACTGGACT GCCTACATTA GCAGAGAGAT CCTCAGGGGG 
CTCACTCATC TTCACCAACA CAAAGTCATC CACAGAGACA TCAAAGGCCA GAACGTGCTG 
CTAACAGAGA ACGCTGAGGT CAAACTAGTG GACTTCGGTG TGAGCGCACA GCTGGACCGA 
ACCGTCAGCC GCAGGAACAC TTTCATCGGC ACCCCTTATT GGATGGCACC AGAGGTCATT 
GCTTGTGATG AGAATCCTGA CGCCACATAT GACTATAAGA GTGACCTGTG GTCTTTGGGA 
ATCACGGCCC TTGAAATGGC TGAAGGAGCT CCACCCCTTT GTGAATTGCA CCCAATGAGA 
GCCCTGTTTC TGATCCCCAG AAATGCTCCT CCCAGGCTCA AGTCCAAGAA GTGGACTAAG 
AGATTCCACT CGTTCATCGA AAGCTGTTTG GTAAAGAACC ACAACATGAG GCCTGGTACA 
GATCAGCTCC TCAAACACCC CTTTATCCGG GACATGGCGA ACGAGAGAAC GATCCGCATC 
CAGTTGAAGG ATCACATCGA TCGCACCAGG AAGAAGAGAG GAGAGAGAGA TGAGACGGAG 
TATGAGTACA GCGGGAGTGA AGAGGAGGAA GAGGCCATGC TTGAGATTGG AGAACCCAGC 
TCCATTATCA ACATACCCGG CGAGTCGACT CTACGGCGGG ATTTCCTGCG CCTGCAGGTG 
GCCAATAAGG AGCGTTCAGA CGCCCTGCGT AGACAACAGC TTGAGCAGCA GCAGAACGAG 
GGACACAAAC ATCAACTGCT GGCAGAGAGG CAGAAACGCA TCGAGGAACA GAAAGAACAG 
AGGCGCCGAT TAGAAGAGCA ACAGCGACGG GAACGTGAAG CAAGAAAACA GCAGGAGCAA 
AGACATCGCT ATGAGGAGCT CCGGCGAGAG GAGGAGAGGA GACAGGCTGA AAGAGAGCAG 
GAGTATAAAC GTAAACAGGT GGAGGAACAA AGACAGGCGG ATAGATTGCA GAGGCAGCTC 
CAGCAAGAAA GAGCTTACCT CGTCTCTCTC CAACAACAGC AACAGCAGCA ACAACAAGAC 
TCCAGACCTG AAGAGAAGAA GCAACTTTAC CATTACAAAG ATGCTGTCAA CCCCAGCGAC 
AAACCAGCGT GGGCTAAAAA GATCCATGGA CCGCCCCAGA TAAAGATCCA ACCCAGCAAC 
CGCAAGCAGT CCACACCCCT CTACTATAAG TTGGACAACC TAGGGATCCC CATAGGCCGC 
GCTATACTGA ATCCCTCAAT TAATCAAAGT GTCATGCAGC CTTTAAAGCC GCAGAAGCAG 
AACCTGATAC AGCGACAATC GATTTCACCA CCCCCCCAAT ACAGTGCAAA AAAAATTAGT 
ATGGGACCTC GCCCAGGCTT TTCCCGTCCT CCTTATAAGC GGGTTGAGGA GCGATCTAAA 
CTGAACCGCC AGAGCTCTCC TGCCCTACAG CATAAAGTGG CCAATCGTAT CTCTGACCCT 
TCCCTCCCGC CTCGCTCTGA GTCCTTCAGC AGTGGAGGAA TGCAGCCAGC ACGCACCCCT 
CCTATGCACC GCCCTGTGGA GCCCCAGATG GCTCATTTGG TTCCAGTGAG GTCCCATAGC 
TCCTCTATGT CAGTCTCGCA GTCTTTGCAT GATCAATCTG CAAAGGGTGT TTCAGCCTTC 
CAGGAAAGCC TGGTGTCCAA TCGACCAGAG ATGCCTCGAC AGAACTCCGA CCCGACTTCA 
GAGATGCCCC CTCCTGCCCC TCGCATGGCC AGCCATGAGG AAAAACCTGA CCGCAGTTCC 
CCCTGGCTGA GGGAAGACGC CGTTCCTCCC AAGTTGTCCG GTACTACCCA GGTGCCCCAG 
AGGACCACTT CTATCTCCCC TGCTCTTGCT AGGAAAAACT CTCCTGGAAA CGGAGAGTCC 
AGTGGAGGGG GGCGGTCGGC CTCTCAGCTC ATTCGCACCA GTAATCCAGA CCTGAGGAGG 
ACAGAGTTGT CTCTGGATGT GGCCCTGCAG AGAACCAGCA GCAACAGCTC CTCCAGCTCC 
AGCACACCCA GCTCTCAGGG TGGATCCCAG CCTGGATCCA GTGAGAGGAA CCAGCCAAGA 
GGATCTGGTA AATCAGAGGG ATCTCCAGTG GCATTACAGG AAACCTCCAA GCCCATTCAG 
GAGGAAGGCA GAGAGCTAAG CAAACCCAGT CGGCCTGCAG ATCTGACTGC CCTGGCTAAA 
GAACTGAGGG AGCTGCGAAT AGATGAGGTC AGTCGACCCC CTCTTAAACT CACAGACTAC 
TCCTCTTCTA GTGAGGACTC TGATAGCAGT GAGGATGAGG ATGGTACGGT GGGACACGAC 
GGCACTGTGT CAGTCAGTGA CATACCCAGG ATCATGCCAT CCGTTCAAGG AAATAATGAG 
TCGTTCACGA GCAGCCATGA TGATTCCCTC ACTGACTCCT ACAATGATGA GTCTAAAGAT 
GGGACACTGA GGATGCGAGA GAACAATGAG AGGCAACGCT TAGGCCATGG CGAGAGCAAT 
GGCTTTGCAG GCCATGGAAA CCTACCAGAC CTCCTGCAGC AGAGCCACTC TCCATCCGGA 
ACTCCAACAG GCCTCAATTC CCAAGACCTG GACTCTATGG ATGAGTTTGG TCTTGGCGGT 
GGTTCGAAAG CTTCTTTCAC TCCTTTTGTG GACCCACGAG TGTATCAAAC CTCCCCCACA 
GACGACAACG ATGAGAGCTC AGCAGCCGCT ATGTTTGCGA ATGAGCTGTT GCGTCAAGAG 
CAGGCCAAAC TGAATGAGGC CCGCAAGATC TCTGTGGTGA ACGTCAACCC CACCAACATC 
AGACCTCACA GCGACACGCC AGAGATCCGC AAATACAAAA AACGCTTTAA CTCCGAGATC 
TTGTGTGCTG CTCTATGGGG TGTTAACCTC TTGGTGGGCA CTGAAAATGG TCTGATGCTG 
CTGGATCGTA GCGGTCAGGG CAAGGTTTAT AACCTCATCA CCCGCAGACG CTTCCAACAG 
ATGGATGTGC TGGAGGGTCT AAATGTCCTG GTCACCATAT CAGGGAAAAA AAATAAGCTC 
CGTGTGTACT ACTTGTCATG GCTAAGAAAC CGAATACTGC ACAATGACCC GGAGGTGGAG 
AAGAAGCAGG GCTGGATCAC TGTTGGTGAA CTCGAAGGCT GCATCCACTA TAAAGTTGTG 
AAATACGAGA GAATCAAATT CCTGGTGATT GCACTGAAGA ACTCGGTAGA GATTTACGCA 
TGGGCCCCCA AGCCGTACCA TAAATTCATG GCTTTCAAGT CGTTCACAGA GCTGCAGCAC 
AGACCTCAGC TGGTGGACCT CACAGTAGAA GAGGGTCAGA GGTTAAAGGT CATCTACGGC 
TCTAGTGTGG GCTTTCACGT CATCGATGTG GATTCCGGAA GCCCCTACGA CATCTACATA 
CCGTCACATA TTCAGAGCAC TGTGACTCCT CATGCCATCG TGGTGCTTCC GAAGACTGAT 
GGCATGGAGA TGCTGCTGTG TTATGAAGAT GAGGGCGTTT ATGTCAATAC CTACGGCAGA 
ATCACCAAGG ACGTTGTCTT GCAGTGGGGA GAGATGCCCA CCTCTGTTGC TTATATTCAT 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCTGT GGAAACTGGT 
CACCTGGATG GGGTTTTCAT GCACAAGAGA GCTCAGAGAC TCAAGTTCTT GTGTGAGAGG 
AACGACAAGG TGTTCTTCGC CTCAGTGCGG TCAGGTGGCA GCAGTCAAGT GTTCTTCATG 
ACCCTGAATA GGAACTCTCT GATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tnika
Entrez Gene ID	325042
Full Name	TRAF2 and NCK interacting kinase a
Synonyms	fc52d11,msn,tnikl,wu:fc52d11,zgc:63535
General protein information	Preferred Names TRAF2 and NCK interacting kinase a Names TRAF2 and NCK interacting kinase a TRAF2 and NCK interacting kinase like misshapen 1 msn1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	TNIK	XP_001164831.1
Gallus gallus (chicken)	TNIK	XP_422794.3
Danio rerio (zebrafish)	tnika	NP_955997.1
Mus musculus (house mouse)	Tnik	NP_081186.1
Xenopus tropicalis (tropical clawed frog)	tnik	XP_002931596.2
Homo sapiens (human)	TNIK	NP_055843.1
	TNIK	XP_001088063.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tnika cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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