gRNAs
The following aars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa36096 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001040035.2 | |
| Accession Version | NM_001040035.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-07-28 | |
| Organism | Danio rerio(zebrafish) | |
| Product | alanine--tRNA ligase, cytoplasmic isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649641.20. On Oct 19, 2010 this sequence version replaced NM_001040035.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' CDS and uses an alternate 3' terminal exon compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus and a distinct, shorter C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC097014.1, BC171667.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001035124.2 |
| CDS | 124..1425 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001040035.2 |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa36098 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001044310.2 | |
| Accession Version | NM_001044310.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-07-28 | |
| Organism | Danio rerio(zebrafish) | |
| Product | alanine--tRNA ligase, cytoplasmic isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649641.20. On Oct 19, 2010 this sequence version replaced NM_001044310.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01000023.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCTCTTC ATCTCACATT TTTATGTGCC AAAGCTTTCT TCTGTTGTCA GTCTCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001037775.1 |
| CDS | 120..3095 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001044310.2 |
1 | ATGGCTCTTC ATCTCACATT TTTATGTGCC AAAGCTTTCT TCTGTTGTCA GTCTCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa23217 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005159050.4 , XM_005159050.3 | |
| Accession Version | XM_005159050.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | alanine--tRNA ligase, cytoplasmic isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159050.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005159107.1 |
| CDS | 124..3024 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005159050.4 |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa23217 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005159050.4 , XM_005159050.3 | |
| Accession Version | XM_005159050.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | alanine--tRNA ligase, cytoplasmic isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005159107.1 |
| CDS | 107..3007 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005159050.3 |
1 | ATGGACTCTT CACTGACTGC TGCACAGATC CGTGAGAAGT TCATCGACTT CTTCCGCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Evolution of complexity in the zebrafish synapse proteome. |
A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases. |
Utilising polymorphisms to achieve allele-specific genome editing in zebrafish. |
Integrative view of ?2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses. |
