chsy1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chsy1
Entrez Gene ID	324407
Full Name	chondroitin sulfate synthase 1
Synonyms	chys1,wu:fc27h05,wu:fc27h06,zgc:64191
General protein information	Preferred Names chondroitin sulfate synthase 1 Names chondroitin sulfate synthase 1 carbohydrate (chondroitin) synthase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212678.1	NP_997843.1	chondroitin sulfate synthase 1 precursor

Pathways from BioSystems

Homologies

	CHSY1	XP_001084554.2
Rattus norvegicus (Norway rat)	Chsy1	NP_001099738.1
Homo sapiens (human)	CHSY1	NP_055733.2
Gallus gallus (chicken)	CHSY1	XP_413889.3
Drosophila melanogaster (fruit fly)	CG9220	NP_996440.2
Canis lupus familiaris (dog)	CHSY1	XP_545821.3
Danio rerio (zebrafish)	chsy1	NP_997843.1
Bos taurus (cattle)	CHSY1	NP_001178086.1
Mus musculus (house mouse)	Chsy1	NP_001074632.1
Xenopus tropicalis (tropical clawed frog)	chsy1	NP_001090724.1
Pan troglodytes (chimpanzee)	CHSY1	XP_523172.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001010	XP_309203.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene.
Geng FS, Abbas L, Baxendale S, Holdsworth CJ, Swanson AG, Slanchev K, Hammerschmidt M, Topczewski J, Whitfield TT
Development (Cambridge, England)140(21)4362-74(2013 Nov)

Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development.
Filipek-G?rniok B, Holmborn K, Haitina T, Habicher J, Oliveira MB, Hellgren C, Eriksson I, Kjell?n L, Kreuger J, Ledin J
Developmental dynamics : an official publication of the American Association of Anatomists242(8)964-75(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chondroitin sulfate expression is required for cardiac atrioventricular canal formation.
Title: Chondroitin sulfate expression is required for cardiac atrioventricular canal formation.

The following chsy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chsy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04389	NM_212678.1 Latest version!	Danio rerio chondroitin sulfate synthase 1 (chsy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chsy1 ( NM_212678.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997843.1 Danio rerio chondroitin sulfate synthase 1 (chsy1), mRNA.

Price & Availability↑

CloneID	ODa04389
Clone ID Related Accession (Same CDS sequence)	NM_212678.1
Accession Version	NM_212678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC064670.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGAA GGAGTCGCAG ATCTTGGTTT AGCGTGTTGC TGGGGCTGGT GGTCGGGTTC 
ACACTGGCGT CCAGGCTCAT CCTGCCCCGG GCCAGCGAGC TGAAGAAAGC GGGTCAGAAG 
CGGCGGGCGA GCGGCGGCGG CGCGGGAGGC TGCGGAGGCG GCGGAGGGAT GATGATGATG 
ATGAAGAAGG AGTACGGAGG GGTCTTGATG CCCGGTACCG AGAAGCCCTC AGTGCCCCAG 
TCTAGCAGCT TTCTGTTTGT GGGCGTCATG ACCGCGCAGA AGTACCTGAA TAACCGCGCC 
GTGGCAGCAT ATAGGACCTG GGCCAAGACC ATCCCGGGCA AGGTGGAGTT TTTCTCCAGT 
GAAGGTTCTG ACACCACCAT CCCCATCCCC ATTGTGGCTC TGCAGAACGT GGATGACTCG 
TACCCTCCGC AGAAGAAGTC CTTCATGATG CTCAAATACA TGCATGACCA CTATTTGGAC 
CAGTTCGAGT GGTTCATGCG AGCCGACGAC GACGTCTACA TCAAAAGCGA AAAGCTGGAG 
AGTTTCCTAA GGAGTCTCAA CAGCAGCGAG GCCATCTTTC TGGGCCAGAC GGGCATGGGA 
GCCCGCGACG AACTGGGCAA ACTGGCGCTG GAGCCGGGGG AGAACTTCTG CATGGGCGGC 
CCTGGTGTTA TCATGAGCCG CGAGGTGCTG CGCAGGATGG TTCCCCACAT CCGCCAGTGC 
CTGCAGGAGA TGTACACCAC CCATGAGGAC GTGGAGGTGG GCCGCTGCGT GCGCAGATTC 
GCCGGAGTGC AGTGTGTTTG GTCCTACGAG ATGCAGCAGC TGTTCTATGA GAATTACGAG 
CCAAACAAGA AGGGCTACAT CCGTGATCTC CACAACAGTA AGATCCACAG AGCCATAACC 
CTTCACCCCA ACAAGAACCC ACCTTACCAG TATCGGTTGC ACAGCTACAT GTTGAGCCGG 
AAGATTGCAG AGCTGCGCCA CCGCACCATT CAGCTCCATC GTGAGATTGT GCAAATGAGC 
CGCTACAGCA ACACCGAGGT GCACAGGGAA GACCTTCAAC TGGGCATGCC TCCATCCTTC 
ATGCGCTTTC ATCCCCACCA ACGTGAGGAG GTGCTGGAAT GGGAATTCCT CACGGGAAAG 
TACATTTTCT CATCCTCAGA TGGACAGCCT CCCCGTCGAG GCATTGACTC CTCGCAGAAG 
ATGGCGCTGG ACGACATCAT CATGCAAGTT ATGGAGATGA TTAACGCCAA TGCCAAGACA 
CGCGGAAGAG TCATTGACTT CAAAGAGATC CAATATGGCT ACCGAAGGGT CAACCCTATG 
TATGGTGCAG AGTACGTACT GGATCTGCTG CTGCTCTACA AGAAGCACAA GGGGAAGACT 
ATGACAGTGC CCGTAAGGAG GCATGCGTAT CTTCAGCAGA CCTTCAGCAA GATCCAGTTC 
TTGGAAGAAG AAGAGATGGA CGCTCGAGTC CTGGCTGCCA GGATCAACCA AGACTCCGAC 
TCGCTGTCTT TCTTGTCCAA CTCCCTCAAG ATGCTCGTCC CGTTCAAGTT GAGCAGCCCA 
GGCATCGAAC AACACGAACC CAAAGAGAAG AAGATCAACA TCCTTGTGCC GCTGGCCGGA 
CGCTACGAGA TTTTTCTGCG CTTCATGGCC AACTTCGAGA AGATCTGCCT CATTCCCAAC 
CAGAATGTCA AGCTTCTGAT TCTGCTTTTC AGCACGGACA ACAACACTGA GCGCATCAAG 
CAAATCGAGC TCATGAGGGA GTATCGCATG AAGTACCCCA AAGCCGATAT GGAGATCAAA 
CCGGTTTCCG GACCATTCTC CCGTGCTCTG GCCCTGGAGG TCGGCTCGGC CCACTTTACC 
AATGATTCTT TACTCTTCTA CTGTGACGTG GACTTACTGT TTACTCCTGA CTTCTTGACT 
CGATGTCGTG GGAATACCAT ACTTGGGGAA CAGACATACT TTCCTATTAT TTTCAGCCAG 
TATGACCCAA AGGTTGTCTA CGCAGGGAAG GTGCCCAGCG ACAACCACTA CGTGTTCACA 
TCCAAAACAG GCCTGTGGAG ACACTATGGT TTTGGTATTG TCTGCGTCTA CAAAGGAGAC 
CTGGTCAAAG CTGGTGGTTT TGACGTCTCC ATCCAAGGTT GGGGACTGGA GGACGTTGAC 
CTTTTCAACA AGTTTGTCCA ATCCGGGATC AAGCTGTTCC GCAGTACGGA TACCGGCATC 
GTTCACGTGC ATCACCCTGT GGTTTGCGAC CCCAATCTTG ATCCCAAGCA GTACAAAATG 
TGCCTGGGAT CCAAAGCATC CTCGCACGGC TCCACGCAGC AACTCGCAGA GCTATGGTTG 
GAGAAAAACA ACCACAGCTT CCGAAAGATC TCGAACTCCA ATAATGAATC AATGAGGACA 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997843.1
CDS	286..2691
Translation	MAGRSRRSWFSVLLGLVVGFTLASRLILPRASELKKAGQKRRASGGGAGGCGGGGGMMMMMKKEYGGVLMPGTEKPSVPQSSSFLFVGVMTAQKYLNNRAVAAYRTWAKTIPGKVEFFSSEGSDTTIPIPIVALQNVDDSYPPQKKSFMMLKYMHDHYLDQFEWFMRADDDVYIKSEKLESFLRSLNSSEAIFLGQTGMGARDELGKLALEPGENFCMGGPGVIMSREVLRRMVPHIRQCLQEMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEPNKKGYIRDLHNSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVQMSRYSNTEVHREDLQLGMPPSFMRFHPHQREEVLEWEFLTGKYIFSSSDGQPPRRGIDSSQKMALDDIIMQVMEMINANAKTRGRVIDFKEIQYGYRRVNPMYGAEYVLDLLLLYKKHKGKTMTVPVRRHAYLQQTFSKIQFLEEEEMDARVLAARINQDSDSLSFLSNSLKMLVPFKLSSPGIEQHEPKEKKINILVPLAGRYEIFLRFMANFEKICLIPNQNVKLLILLFSTDNNTERIKQIELMREYRMKYPKADMEIKPVSGPFSRALALEVGSAHFTNDSLLFYCDVDLLFTPDFLTRCRGNTILGEQTYFPIIFSQYDPKVVYAGKVPSDNHYVFTSKTGLWRHYGFGIVCVYKGDLVKAGGFDVSIQGWGLEDVDLFNKFVQSGIKLFRSTDTGIVHVHHPVVCDPNLDPKQYKMCLGSKASSHGSTQQLAELWLEKNNHSFRKISNSNNESMRTE

Target ORF information:

RefSeq Version	NM_212678.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chondroitin sulfate synthase 1 (chsy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212678.1

ORF Insert Sequence:

ATGGCAGGAA GGAGTCGCAG ATCTTGGTTT AGCGTGTTGC TGGGGCTGGT GGTCGGGTTC 
ACACTGGCGT CCAGGCTCAT CCTGCCCCGG GCCAGCGAGC TGAAGAAAGC GGGTCAGAAG 
CGGCGGGCGA GCGGCGGCGG CGCGGGAGGC TGCGGAGGCG GCGGAGGGAT GATGATGATG 
ATGAAGAAGG AGTACGGAGG GGTCTTGATG CCCGGTACCG AGAAGCCCTC AGTGCCCCAG 
TCTAGCAGCT TTCTGTTTGT GGGCGTCATG ACCGCGCAGA AGTACCTGAA TAACCGCGCC 
GTGGCAGCAT ATAGGACCTG GGCCAAGACC ATCCCGGGCA AGGTGGAGTT TTTCTCCAGT 
GAAGGTTCTG ACACCACCAT CCCCATCCCC ATTGTGGCTC TGCAGAACGT GGATGACTCG 
TACCCTCCGC AGAAGAAGTC CTTCATGATG CTCAAATACA TGCATGACCA CTATTTGGAC 
CAGTTCGAGT GGTTCATGCG AGCCGACGAC GACGTCTACA TCAAAAGCGA AAAGCTGGAG 
AGTTTCCTAA GGAGTCTCAA CAGCAGCGAG GCCATCTTTC TGGGCCAGAC GGGCATGGGA 
GCCCGCGACG AACTGGGCAA ACTGGCGCTG GAGCCGGGGG AGAACTTCTG CATGGGCGGC 
CCTGGTGTTA TCATGAGCCG CGAGGTGCTG CGCAGGATGG TTCCCCACAT CCGCCAGTGC 
CTGCAGGAGA TGTACACCAC CCATGAGGAC GTGGAGGTGG GCCGCTGCGT GCGCAGATTC 
GCCGGAGTGC AGTGTGTTTG GTCCTACGAG ATGCAGCAGC TGTTCTATGA GAATTACGAG 
CCAAACAAGA AGGGCTACAT CCGTGATCTC CACAACAGTA AGATCCACAG AGCCATAACC 
CTTCACCCCA ACAAGAACCC ACCTTACCAG TATCGGTTGC ACAGCTACAT GTTGAGCCGG 
AAGATTGCAG AGCTGCGCCA CCGCACCATT CAGCTCCATC GTGAGATTGT GCAAATGAGC 
CGCTACAGCA ACACCGAGGT GCACAGGGAA GACCTTCAAC TGGGCATGCC TCCATCCTTC 
ATGCGCTTTC ATCCCCACCA ACGTGAGGAG GTGCTGGAAT GGGAATTCCT CACGGGAAAG 
TACATTTTCT CATCCTCAGA TGGACAGCCT CCCCGTCGAG GCATTGACTC CTCGCAGAAG 
ATGGCGCTGG ACGACATCAT CATGCAAGTT ATGGAGATGA TTAACGCCAA TGCCAAGACA 
CGCGGAAGAG TCATTGACTT CAAAGAGATC CAATATGGCT ACCGAAGGGT CAACCCTATG 
TATGGTGCAG AGTACGTACT GGATCTGCTG CTGCTCTACA AGAAGCACAA GGGGAAGACT 
ATGACAGTGC CCGTAAGGAG GCATGCGTAT CTTCAGCAGA CCTTCAGCAA GATCCAGTTC 
TTGGAAGAAG AAGAGATGGA CGCTCGAGTC CTGGCTGCCA GGATCAACCA AGACTCCGAC 
TCGCTGTCTT TCTTGTCCAA CTCCCTCAAG ATGCTCGTCC CGTTCAAGTT GAGCAGCCCA 
GGCATCGAAC AACACGAACC CAAAGAGAAG AAGATCAACA TCCTTGTGCC GCTGGCCGGA 
CGCTACGAGA TTTTTCTGCG CTTCATGGCC AACTTCGAGA AGATCTGCCT CATTCCCAAC 
CAGAATGTCA AGCTTCTGAT TCTGCTTTTC AGCACGGACA ACAACACTGA GCGCATCAAG 
CAAATCGAGC TCATGAGGGA GTATCGCATG AAGTACCCCA AAGCCGATAT GGAGATCAAA 
CCGGTTTCCG GACCATTCTC CCGTGCTCTG GCCCTGGAGG TCGGCTCGGC CCACTTTACC 
AATGATTCTT TACTCTTCTA CTGTGACGTG GACTTACTGT TTACTCCTGA CTTCTTGACT 
CGATGTCGTG GGAATACCAT ACTTGGGGAA CAGACATACT TTCCTATTAT TTTCAGCCAG 
TATGACCCAA AGGTTGTCTA CGCAGGGAAG GTGCCCAGCG ACAACCACTA CGTGTTCACA 
TCCAAAACAG GCCTGTGGAG ACACTATGGT TTTGGTATTG TCTGCGTCTA CAAAGGAGAC 
CTGGTCAAAG CTGGTGGTTT TGACGTCTCC ATCCAAGGTT GGGGACTGGA GGACGTTGAC 
CTTTTCAACA AGTTTGTCCA ATCCGGGATC AAGCTGTTCC GCAGTACGGA TACCGGCATC 
GTTCACGTGC ATCACCCTGT GGTTTGCGAC CCCAATCTTG ATCCCAAGCA GTACAAAATG 
TGCCTGGGAT CCAAAGCATC CTCGCACGGC TCCACGCAGC AACTCGCAGA GCTATGGTTG 
GAGAAAAACA ACCACAGCTT CCGAAAGATC TCGAACTCCA ATAATGAATC AATGAGGACA 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene.
Development (Cambridge, England)140(21)4362-74(2013 Nov)
Geng FS,Abbas L,Baxendale S,Holdsworth CJ,Swanson AG,Slanchev K,Hammerschmidt M,Topczewski J,Whitfield TT

Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development.
Developmental dynamics : an official publication of the American Association of Anatomists242(8)964-75(2013 Aug)
Filipek-G?rniok B,Holmborn K,Haitina T,Habicher J,Oliveira MB,Hellgren C,Eriksson I,Kjell?n L,Kreuger J,Ledin J

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chsy1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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