top2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	top2a
Entrez Gene ID	323733
Full Name	DNA topoisomerase II alpha
Synonyms	fc08c07,wu:fc08c07
General protein information	Preferred Names DNA topoisomerase II alpha Names DNA topoisomerase 2-alpha topoisomerase (DNA) II alpha topoisomerase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310015.1	NP_001296944.1	DNA topoisomerase 2-alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Liu Q, Klingler RH, Wimpee B, Dellinger M, King-Heiden T, Grzybowski J, Gerstenberger SL, Weber DN, Carvan MJ 3rd
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)

Single-Cell RNA-Sequencing Reveals a Continuous Spectrum of Differentiation in Hematopoietic Cells.
Macaulay IC, Svensson V, Labalette C, Ferreira L, Hamey F, Voet T, Teichmann SA, Cvejic A
Cell reports14(4)966-977(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish models of human liver development and disease.
Wilkins BJ, Pack M
Comprehensive Physiology3(3)1213-30(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The absence of zygotic top2a is not fully compensated for by maternal, zygotic or ectopic top2b mRNA suggesting distinct functions of Top2a and Top2b in embryonic development.
Title: Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.

Results confirm a conserved role for topoisomerase II alpha (TOP2A) in vertebrates as well as a dose-sensitive requirement for top2a in adults.
Title: Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish.

The following top2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39788	NM_001310015.1 Latest version!	Danio rerio DNA topoisomerase II alpha (top2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top2a ( NM_001310015.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296944.1 Danio rerio DNA topoisomerase II alpha (top2a), mRNA.

Price & Availability↑

CloneID	ODa39788
Clone ID Related Accession (Same CDS sequence)	NM_001310015.1
Accession Version	NM_001310015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 2-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FO704786.2, BX470262.7, EB928159.1, EH541591.1 and EH551427.1. On Jun 21, 2015 this sequence version replaced XM_009306487.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01005796.1, BC086970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAA CAGAAGGACC TCTGAAGAGT TTATTTGAGA ATAAGGCTCT CAGTAAGCCC 
AAGAAAGATG AGAAGCGTCT GTCTGTGGAA CGGATCTATC AGAAGAAGAC CCAGCTGGAG 
CATATCCTGC TTCGACCTGA CACCTACATA GGCTCAGTGG AACCTGTGAC TCAGCAAATG 
TGGGTGTTTG ATGAGGATAC AGGGATGAAT TGCAGAGACA TCACATTTGT GCCAGGACTC 
TACAAGATTT TTGATGAGAT TCTGGTGAAT GCTGCTGATA ACAAACAAAG GGACAAGACT 
ATGAACTGCA TCAAGGTCAA CATTGACCCA GAGAATAACA CAATCAGTGT GTGGAACAAT 
GGGAAGGGTA TTCCTGTGGT TGAACACAAG GTGGAAAAAG TGTATGTTCC TGCTTTGATC 
TTTGGCCAAC TCCTGACCTC CAGTAACTAT GATGATGATG AGAAGAAGGT CACAGGTGGG 
CGAAATGGAT ATGGTGCAAA GCTGTGTAAC ATCTTCAGCC TAAAGTTTAC TGTGGAGACT 
GCTTGCAGAG AGTCAAAACG CTGTTTCAAA CAGACATGGT TTGACAACAT GGGACGAGCA 
GGTGAATCTA AGATTAAACC CTTCGACGGT GACGACTACA CCTGCATCAC ATTCCAGCCA 
GACCTGGCCA AGTTCAAGAT GCAAGCTTTA GATAAGGACA CTGTTGCGCT ACTGACTCGA 
CGAGCATATG ACATTGCTGG ATCCACTAAA GGTGTCCGTG TCTTACTTAA TGGAAAGAGG 
CTGCCAATTA ATAATTTCCG CACCTATGTG GACTTGTATG TGAAGGATAA GGTGGATGAA 
ACTGGTTCTC CCCTGACAGT AGTTCACGAG ATGGTGAATG AGCGCTGGGA AGTGTGTCTT 
ACCCTTAGTG AAAAAGGGTT CCAGCAAGTC AGCTTTGTTA ACAGCATCGC CACAACTAAG 
GGTGGCAGAC ACACAGACTA TGTTGCAGAT CAGATTGTGT CCAAACTGAT TGAAGTAGTG 
AAAAAGAAGA ACAAGGCTGG AGTAGCCGTC AAACCTTTCC AGGTCAAGAA CCATATGTGG 
TTGTTTGTGA ACTGTCTGAT AGAGAACCCC AGCTTTGACT CCCAGACGAA AGAGAATATG 
ACCTTGCAGC AGAAGAATTT TGGTTCTGCC TGCTCTCTGA GTGACAAGTT TGTTAAGCAG 
GCTAATGGGT GTGGAATTGT GGAAAGCATT ATGAACTGGG TGAAGTTTAA AGCCCAGGCT 
CAGCTCAATA AGAAATGCTC AGCTGTCAAA CACACGAAGA TTAAAGGAGT ACCTAAGCTG 
GATGATGCCA ACGATGCAGG TGGAAAGAAC TCCAATAGCT GCACTCTAAT CCTGACCGAA 
GGAGACTCGG CCAAAACTCT GGCCGTGTCG GGTCTGGGTG TTGTGGGACG TGATCGATAT 
GGTGTCTTCC CTCTGCGTGG TAAAATGCTC AACGTTCGAG AAGCTTCACA CAAACAGATC 
ATGGAAAATG CTGAGATCAA CAACATCATT AAGATCCTGG GTCTGCAGTA CAAAAAGAAC 
TACAGTGACC CTGAGTCACT CAAGAGTCTG CGCTATGGCA AGCTCATGAT CATGACAGAT 
CAGGATCAAG ATGGATCTCA CATTAAAGGT CTGCTGATCA ACTTCATCCA TCATAACTGG 
CCATCACTGC TGCGCCATAA CTTTCTAGAA GAGTTCATCA CCCCTATCAT CAAGGCATCC 
AATAAGAAGC AGGAGATTTC TTTCTACAGT ATTCCTGAGT TCACTGAGTG GAAAGAGAAG 
CAGAGTAGCA TCAAATCCTG GAAAATCAAA TACTACAAAG GTTTGGGTAC CAGCACATCA 
AAGGAGGCCA AAGAGTATTT TTCGGATATG ACAAGACATC GAATCCCTTT TAAGTACTCC 
GGGCCGGCTG ATGATGAGGC TATCACCCTG GCCTTTAGTA AGAAGATGAT AGAGGAAAGG 
AAGGAATGGC TGACCAGCTT TATGATCAAC AGACGTCAAC GTAGAGAACA CAACCTGCCT 
GAGGAGTATC TGTATGGACA GGAAACCAAG TCCCTCTCCT ACCATGACTT TATCAATAAA 
GAGCTTGTGT TGTTCTCCAA CTCTGACAAC GAGAGGTCAA TCCCCTGCAT GGTGGATGGG 
CTTAAACCAG GCCAGAGGAA AGTTGTCTTC TGTTGTTTTA AGAGAAATGA TAAGAGAGAG 
GTGAAAGTGG CTCAGTTGGC TGGATCAGTG GCTGAGATGT CAGCATATCA CCATGGAGAG 
GTCTCTCTGA TGATGACCAT TGTGGGTCTT GCTCAGAATT TTGTGGGCAG TAATAATTTG 
AACCTGCTCC AGCCTCTGGG TCAGTTTGGC ACACGTCTCC ATGGCGGCAA AGACTCAGCC 
AGCCCCAGAT ACATCTTTAC TATGCTCAGT ACACTTGCTC GTCTACTGTT CCCTGTGGTG 
GACGACAACC TGCTGAAGTA CAACTACGAT GACAATTTGC GAGTGGAACC GGAGTGGTAC 
ATGCCCATCA TCCCTCTGGT GCTGGCTAAT GGAGGTGAAG GCATTGGCAC AGGATGGGCC 
AGCCGCATCC CTAACTATGA CGTGCGAGAG ATCATCAACA ACATTCACCG CATGCTTAAC 
GGAGAAGAGC CCCTCCCCAT GCTGCCCAGC TATAAGGGCT TCAAGGGCAC CATTGATGAG 
CTGGCAAAGA ATCAGTATGT GAATAATGGA GAAGTGGCCA TCATTGACTC CACCACCATC 
GAGATCACAG AGCTGCCTGT CAAAACCTGG ACACAGACCT ATAAGGAGAA TGTGTTGGAG 
GTGATGCTGA ACGGCACAGA GAAGGTCCCT GCTCTGATCA CAGACTACAA GGAGTATCAT 
ACAGACACCA CAGTGCGTTT TGTTGTTAAG ATGACAGAGG AGCGCCTGAG AGAGGCAGAG 
GCAGCTGGAC TGCACAAAGT CTTTAAACTC CAGAACCTGC TGACCTGTAA CTCCATGGTG 
TTATTTGATC ATGTGGGCAG TCTGAAGAAG TATGAGTCTG TTCAGGACAT CTTAAAGGAG 
TTCTTTGAGC TCAGAATGAA ATACTACGTC CTCAGGAAAG ACTGGCTTGT AGGAATGTTG 
GGAGCTGAAA GTGCCAAACT TTCCAACCAA GCCCGATTTA TTCTGGAGAA GATCCAGGGC 
ACACTGGTCA TTGAAAACAA ACCGAAAAAA GAGCTGATCC GCATGCTTCA GCAGATGGGC 
TATGACTCGG ACCCTGTTAA AGCATGGAAA GATGCTCAGG AGAAGGAAGA AGAAGAGGAT 
GCTGAGGATG ATGATGAGAA GGAGAAAGAG GACACGTCTG GCCCTGATTA TAACTATCTG 
CTCAGCATGC CCATGTGGTA CCTAACCAAA GAAAAGAAAG ATGAGTTGTG CAAGCAGAGA 
GATGCAAAGA TGACTGAGTT GAACACACTC AAGATGAAGG CCCCTGCTGA TCTGTGGAAA 
GAAGATCTGG CAGCTTTTAC TGAGGAGCTG GAGCGTGTGG AACAGAAAGA GAAGGAGTCA 
CAGAATGCAA TGCCCCTGGT GAAGGGTAAA GTGGGCAAAC CAAAAGTGGT CAAGGTAAAG 
AACGAGACCA TGCCTACACC GCAAGGCCGC AGAGTTATCC CCCGCATCAC CAGCACCATG 
AAGGTCCAGG CTGTAAAAAA AGAGCGAGGC AAAGGATCGA AGAAAGAGGC TGGTTTGGTG 
ATGAAGATGG AGTTTGATGA TGAGGCAGAC GCAGCAGCTG ATGGCACTGG GGTGGGATTG 
GCTGCAAGAC TTACAAAGAA GATCAAGAAA GAGCCAACCA AAGACAAAGG CTCAAAGTCA 
GGAAAGCAGA CCACCCTCAC TTTTAAACCA GTCCAGAAGA AGAACCCCTG GTCTTCTGAT 
GAGGGAGAGT CTGATTCTGA CCTAGAGTTG ATGTCTGAGG AAACAGCCCC AAGAGAAAAG 
AGAGAACGCA GAGCCGCTGT TGAAAAGAAG TACAGTCTGT CAAGCAGTGA GGATGAATTT 
GATGACTTTA CGAAGAGCAG TAAAACGAAG CGTGCTGTGA TTGAGGACGA CGAGTCTTTC 
AATCCAGAAC CCAGTGCTGC ACCTGACAGT GATGTGGACT CTCCTCCTCC ACCTGTTGTG 
AAAAAAGCCC CAACAAAAGT TGCCAAAGCA AAGGCAGAGA AACCAGAAGC CAGCAGCAAG 
TCTAGCTCTC ATTCTGAAGC CAGTCAAGAG CCGGCTCCTA AACCTGTGAA GAAAACTGCT 
GAAGGAGCTG CCAAAAAACC TCCTGCCAAG ATGACAAGTG CCGTTTCAAA GAAGAAAGCA 
GCTGATTCAA AACAGCCTTC GATCCTTGAC GTGCTCTCCA AGGCAAGCCC TAAGAAGTCC 
AAAGTTGTCA AAAAGCCAAC TGCTGTCAAG CCATTGTCCA GCGACTCGGA CAGCGCAGGC 
CCTGCTGTCA CAAAGAAACA AGCCAAGAAG AGGAAGACTG TGCTGAGCAG TGACCAATCA 
GACAGCTCCG ACTCTGACAG CGGCAATCTC ATGTCCCGCC TCAAGGGCAA GAGCACTGCT 
GGAAAGAAAG CAAAGAGCTG GGAGGATGAT GAAAGCTTTC AGTTGGAAGA GACTGAGAAG 
GTGGTGGTCT CTGCAGCCCC CAGGAGCCGA GCTGCCCGCA CCACTAAACC TGTGACCTAC 
GCACTCGATT CAGACTCTGA CGATGAAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296944.1
CDS	205..4917
Translation	MAGTEGPLKSLFENKALSKPKKDEKRLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDTGMNCRDITFVPGLYKIFDEILVNAADNKQRDKTMNCIKVNIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSLKFTVETACRESKRCFKQTWFDNMGRAGESKIKPFDGDDYTCITFQPDLAKFKMQALDKDTVALLTRRAYDIAGSTKGVRVLLNGKRLPINNFRTYVDLYVKDKVDETGSPLTVVHEMVNERWEVCLTLSEKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKKNKAGVAVKPFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKNFGSACSLSDKFVKQANGCGIVESIMNWVKFKAQAQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSNSCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKILGLQYKKNYSDPESLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKQEISFYSIPEFTEWKEKQSSIKSWKIKYYKGLGTSTSKEAKEYFSDMTRHRIPFKYSGPADDEAITLAFSKKMIEERKEWLTSFMINRRQRREHNLPEEYLYGQETKSLSYHDFINKELVLFSNSDNERSIPCMVDGLKPGQRKVVFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKEEEEDAEDDDEKEKEDTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKEKESQNAMPLVKGKVGKPKVVKVKNETMPTPQGRRVIPRITSTMKVQAVKKERGKGSKKEAGLVMKMEFDDEADAAADGTGVGLAARLTKKIKKEPTKDKGSKSGKQTTLTFKPVQKKNPWSSDEGESDSDLELMSEETAPREKRERRAAVEKKYSLSSSEDEFDDFTKSSKTKRAVIEDDESFNPEPSAAPDSDVDSPPPPVVKKAPTKVAKAKAEKPEASSKSSSHSEASQEPAPKPVKKTAEGAAKKPPAKMTSAVSKKKAADSKQPSILDVLSKASPKKSKVVKKPTAVKPLSSDSDSAGPAVTKKQAKKRKTVLSSDQSDSSDSDSGNLMSRLKGKSTAGKKAKSWEDDESFQLEETEKVVVSAAPRSRAARTTKPVTYALDSDSDDED

Target ORF information:

RefSeq Version	NM_001310015.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA topoisomerase II alpha (top2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310015.1

ORF Insert Sequence:

ATGGCTGGAA CAGAAGGACC TCTGAAGAGT TTATTTGAGA ATAAGGCTCT CAGTAAGCCC 
AAGAAAGATG AGAAGCGTCT GTCTGTGGAA CGGATCTATC AGAAGAAGAC CCAGCTGGAG 
CATATCCTGC TTCGACCTGA CACCTACATA GGCTCAGTGG AACCTGTGAC TCAGCAAATG 
TGGGTGTTTG ATGAGGATAC AGGGATGAAT TGCAGAGACA TCACATTTGT GCCAGGACTC 
TACAAGATTT TTGATGAGAT TCTGGTGAAT GCTGCTGATA ACAAACAAAG GGACAAGACT 
ATGAACTGCA TCAAGGTCAA CATTGACCCA GAGAATAACA CAATCAGTGT GTGGAACAAT 
GGGAAGGGTA TTCCTGTGGT TGAACACAAG GTGGAAAAAG TGTATGTTCC TGCTTTGATC 
TTTGGCCAAC TCCTGACCTC CAGTAACTAT GATGATGATG AGAAGAAGGT CACAGGTGGG 
CGAAATGGAT ATGGTGCAAA GCTGTGTAAC ATCTTCAGCC TAAAGTTTAC TGTGGAGACT 
GCTTGCAGAG AGTCAAAACG CTGTTTCAAA CAGACATGGT TTGACAACAT GGGACGAGCA 
GGTGAATCTA AGATTAAACC CTTCGACGGT GACGACTACA CCTGCATCAC ATTCCAGCCA 
GACCTGGCCA AGTTCAAGAT GCAAGCTTTA GATAAGGACA CTGTTGCGCT ACTGACTCGA 
CGAGCATATG ACATTGCTGG ATCCACTAAA GGTGTCCGTG TCTTACTTAA TGGAAAGAGG 
CTGCCAATTA ATAATTTCCG CACCTATGTG GACTTGTATG TGAAGGATAA GGTGGATGAA 
ACTGGTTCTC CCCTGACAGT AGTTCACGAG ATGGTGAATG AGCGCTGGGA AGTGTGTCTT 
ACCCTTAGTG AAAAAGGGTT CCAGCAAGTC AGCTTTGTTA ACAGCATCGC CACAACTAAG 
GGTGGCAGAC ACACAGACTA TGTTGCAGAT CAGATTGTGT CCAAACTGAT TGAAGTAGTG 
AAAAAGAAGA ACAAGGCTGG AGTAGCCGTC AAACCTTTCC AGGTCAAGAA CCATATGTGG 
TTGTTTGTGA ACTGTCTGAT AGAGAACCCC AGCTTTGACT CCCAGACGAA AGAGAATATG 
ACCTTGCAGC AGAAGAATTT TGGTTCTGCC TGCTCTCTGA GTGACAAGTT TGTTAAGCAG 
GCTAATGGGT GTGGAATTGT GGAAAGCATT ATGAACTGGG TGAAGTTTAA AGCCCAGGCT 
CAGCTCAATA AGAAATGCTC AGCTGTCAAA CACACGAAGA TTAAAGGAGT ACCTAAGCTG 
GATGATGCCA ACGATGCAGG TGGAAAGAAC TCCAATAGCT GCACTCTAAT CCTGACCGAA 
GGAGACTCGG CCAAAACTCT GGCCGTGTCG GGTCTGGGTG TTGTGGGACG TGATCGATAT 
GGTGTCTTCC CTCTGCGTGG TAAAATGCTC AACGTTCGAG AAGCTTCACA CAAACAGATC 
ATGGAAAATG CTGAGATCAA CAACATCATT AAGATCCTGG GTCTGCAGTA CAAAAAGAAC 
TACAGTGACC CTGAGTCACT CAAGAGTCTG CGCTATGGCA AGCTCATGAT CATGACAGAT 
CAGGATCAAG ATGGATCTCA CATTAAAGGT CTGCTGATCA ACTTCATCCA TCATAACTGG 
CCATCACTGC TGCGCCATAA CTTTCTAGAA GAGTTCATCA CCCCTATCAT CAAGGCATCC 
AATAAGAAGC AGGAGATTTC TTTCTACAGT ATTCCTGAGT TCACTGAGTG GAAAGAGAAG 
CAGAGTAGCA TCAAATCCTG GAAAATCAAA TACTACAAAG GTTTGGGTAC CAGCACATCA 
AAGGAGGCCA AAGAGTATTT TTCGGATATG ACAAGACATC GAATCCCTTT TAAGTACTCC 
GGGCCGGCTG ATGATGAGGC TATCACCCTG GCCTTTAGTA AGAAGATGAT AGAGGAAAGG 
AAGGAATGGC TGACCAGCTT TATGATCAAC AGACGTCAAC GTAGAGAACA CAACCTGCCT 
GAGGAGTATC TGTATGGACA GGAAACCAAG TCCCTCTCCT ACCATGACTT TATCAATAAA 
GAGCTTGTGT TGTTCTCCAA CTCTGACAAC GAGAGGTCAA TCCCCTGCAT GGTGGATGGG 
CTTAAACCAG GCCAGAGGAA AGTTGTCTTC TGTTGTTTTA AGAGAAATGA TAAGAGAGAG 
GTGAAAGTGG CTCAGTTGGC TGGATCAGTG GCTGAGATGT CAGCATATCA CCATGGAGAG 
GTCTCTCTGA TGATGACCAT TGTGGGTCTT GCTCAGAATT TTGTGGGCAG TAATAATTTG 
AACCTGCTCC AGCCTCTGGG TCAGTTTGGC ACACGTCTCC ATGGCGGCAA AGACTCAGCC 
AGCCCCAGAT ACATCTTTAC TATGCTCAGT ACACTTGCTC GTCTACTGTT CCCTGTGGTG 
GACGACAACC TGCTGAAGTA CAACTACGAT GACAATTTGC GAGTGGAACC GGAGTGGTAC 
ATGCCCATCA TCCCTCTGGT GCTGGCTAAT GGAGGTGAAG GCATTGGCAC AGGATGGGCC 
AGCCGCATCC CTAACTATGA CGTGCGAGAG ATCATCAACA ACATTCACCG CATGCTTAAC 
GGAGAAGAGC CCCTCCCCAT GCTGCCCAGC TATAAGGGCT TCAAGGGCAC CATTGATGAG 
CTGGCAAAGA ATCAGTATGT GAATAATGGA GAAGTGGCCA TCATTGACTC CACCACCATC 
GAGATCACAG AGCTGCCTGT CAAAACCTGG ACACAGACCT ATAAGGAGAA TGTGTTGGAG 
GTGATGCTGA ACGGCACAGA GAAGGTCCCT GCTCTGATCA CAGACTACAA GGAGTATCAT 
ACAGACACCA CAGTGCGTTT TGTTGTTAAG ATGACAGAGG AGCGCCTGAG AGAGGCAGAG 
GCAGCTGGAC TGCACAAAGT CTTTAAACTC CAGAACCTGC TGACCTGTAA CTCCATGGTG 
TTATTTGATC ATGTGGGCAG TCTGAAGAAG TATGAGTCTG TTCAGGACAT CTTAAAGGAG 
TTCTTTGAGC TCAGAATGAA ATACTACGTC CTCAGGAAAG ACTGGCTTGT AGGAATGTTG 
GGAGCTGAAA GTGCCAAACT TTCCAACCAA GCCCGATTTA TTCTGGAGAA GATCCAGGGC 
ACACTGGTCA TTGAAAACAA ACCGAAAAAA GAGCTGATCC GCATGCTTCA GCAGATGGGC 
TATGACTCGG ACCCTGTTAA AGCATGGAAA GATGCTCAGG AGAAGGAAGA AGAAGAGGAT 
GCTGAGGATG ATGATGAGAA GGAGAAAGAG GACACGTCTG GCCCTGATTA TAACTATCTG 
CTCAGCATGC CCATGTGGTA CCTAACCAAA GAAAAGAAAG ATGAGTTGTG CAAGCAGAGA 
GATGCAAAGA TGACTGAGTT GAACACACTC AAGATGAAGG CCCCTGCTGA TCTGTGGAAA 
GAAGATCTGG CAGCTTTTAC TGAGGAGCTG GAGCGTGTGG AACAGAAAGA GAAGGAGTCA 
CAGAATGCAA TGCCCCTGGT GAAGGGTAAA GTGGGCAAAC CAAAAGTGGT CAAGGTAAAG 
AACGAGACCA TGCCTACACC GCAAGGCCGC AGAGTTATCC CCCGCATCAC CAGCACCATG 
AAGGTCCAGG CTGTAAAAAA AGAGCGAGGC AAAGGATCGA AGAAAGAGGC TGGTTTGGTG 
ATGAAGATGG AGTTTGATGA TGAGGCAGAC GCAGCAGCTG ATGGCACTGG GGTGGGATTG 
GCTGCAAGAC TTACAAAGAA GATCAAGAAA GAGCCAACCA AAGACAAAGG CTCAAAGTCA 
GGAAAGCAGA CCACCCTCAC TTTTAAACCA GTCCAGAAGA AGAACCCCTG GTCTTCTGAT 
GAGGGAGAGT CTGATTCTGA CCTAGAGTTG ATGTCTGAGG AAACAGCCCC AAGAGAAAAG 
AGAGAACGCA GAGCCGCTGT TGAAAAGAAG TACAGTCTGT CAAGCAGTGA GGATGAATTT 
GATGACTTTA CGAAGAGCAG TAAAACGAAG CGTGCTGTGA TTGAGGACGA CGAGTCTTTC 
AATCCAGAAC CCAGTGCTGC ACCTGACAGT GATGTGGACT CTCCTCCTCC ACCTGTTGTG 
AAAAAAGCCC CAACAAAAGT TGCCAAAGCA AAGGCAGAGA AACCAGAAGC CAGCAGCAAG 
TCTAGCTCTC ATTCTGAAGC CAGTCAAGAG CCGGCTCCTA AACCTGTGAA GAAAACTGCT 
GAAGGAGCTG CCAAAAAACC TCCTGCCAAG ATGACAAGTG CCGTTTCAAA GAAGAAAGCA 
GCTGATTCAA AACAGCCTTC GATCCTTGAC GTGCTCTCCA AGGCAAGCCC TAAGAAGTCC 
AAAGTTGTCA AAAAGCCAAC TGCTGTCAAG CCATTGTCCA GCGACTCGGA CAGCGCAGGC 
CCTGCTGTCA CAAAGAAACA AGCCAAGAAG AGGAAGACTG TGCTGAGCAG TGACCAATCA 
GACAGCTCCG ACTCTGACAG CGGCAATCTC ATGTCCCGCC TCAAGGGCAA GAGCACTGCT 
GGAAAGAAAG CAAAGAGCTG GGAGGATGAT GAAAGCTTTC AGTTGGAAGA GACTGAGAAG 
GTGGTGGTCT CTGCAGCCCC CAGGAGCCGA GCTGCCCGCA CCACTAAACC TGTGACCTAC 
GCACTCGATT CAGACTCTGA CGATGAAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)
Liu Q,Klingler RH,Wimpee B,Dellinger M,King-Heiden T,Grzybowski J,Gerstenberger SL,Weber DN,Carvan MJ 3rd

Single-Cell RNA-Sequencing Reveals a Continuous Spectrum of Differentiation in Hematopoietic Cells.
Cell reports14(4)966-977(2016 Feb)
Macaulay IC,Svensson V,Labalette C,Ferreira L,Hamey F,Voet T,Teichmann SA,Cvejic A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish models of human liver development and disease.
Comprehensive Physiology3(3)1213-30(2013 Jul)
Wilkins BJ,Pack M

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top2a cDNA ORF clone, Danio rerio(zebrafish)

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