lgi1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lgi1a
Entrez Gene ID	323011
Full Name	leucine-rich, glioma inactivated 1a
Synonyms	LGIa,fb78h12,wu:fb78h12,zgc:65886
General protein information	Preferred Names leucine-rich, glioma inactivated 1a Names leucine-rich, glioma inactivated 1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199627.2	NP_955921.2	leucine-rich, glioma inactivated 1a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LGI1	NP_005088.1
Gallus gallus (chicken)	LGI1	NP_001038120.1
Danio rerio (zebrafish)	lgi1a	NP_955921.1
Xenopus tropicalis (tropical clawed frog)	lgi1	NP_001072366.1
Pan troglodytes (chimpanzee)	LGI1	NP_001065246.1
Canis lupus familiaris (dog)	LGI1	XP_534971.2
Rattus norvegicus (Norway rat)	Lgi1	NP_665712.1
Danio rerio (zebrafish)	lgi1b	NP_001122241.1
	LGI1	XP_001093284.1
Bos taurus (cattle)	LGI1	NP_001040056.2
Mus musculus (house mouse)	Lgi1	NP_064674.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Functional phylogenetic analysis of LGI proteins identifies an interaction motif crucial for myelination.
Kegel L, Jaegle M, Driegen S, Aunin E, Leslie K, Fukata Y, Watanabe M, Fukata M, Meijer D
Development (Cambridge, England)141(8)1749-56(2014 Apr)

Loss of zebrafish lgi1b leads to hydrocephalus and sensitization to pentylenetetrazol induced seizure-like behavior.
Teng Y, Xie X, Walker S, Saxena M, Kozlowski DJ, Mumm JS, Cowell JK
PloS one6(9)e24596(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of zebrafish Lgi1a results in abnormal development, brain defects and a seizure-like behavioral phenotype.
Title: Knockdown of zebrafish Lgi1a results in abnormal development, brain defects and a seizure-like behavioral phenotype.

The following lgi1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lgi1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53219	NM_199627.2 Latest version!	Danio rerio leucine-rich, glioma inactivated 1a (lgi1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lgi1a ( NM_199627.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955921.2 Danio rerio leucine-rich, glioma inactivated 1a (lgi1a), mRNA.

Price & Availability↑

CloneID	ODa53219
Clone ID Related Accession (Same CDS sequence)	NM_199627.2
Accession Version	NM_199627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	leucine-rich, glioma inactivated 1a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU570800.2 and BC056753.1. On Nov 5, 2015 this sequence version replaced NM_199627.1. ##Evidence-Data-START## Transcript exon combination :: BC056753.1, GFIL01014404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTGTT TGCGGCGGGC GGCGGTGCTG TGCGTGCTGC TGGCGGCGCT GGTGCTCGGC 
GGCCGGAGGA GGAGCTCCCG CTGCCCCGCG CCCTGCACCT GCAGCAAGGA TAACGCGTTA 
TGCGCCAATA CCGGAGCCAT ACCGAGGAGC TTCCCGCAGG ACGTCATCTC ACTATCATTC 
GTCAAATCCG GCTTTACTGA GATCCCCAAA GAGAGTTTCA TTCACACACC TGCACTACAC 
CTGCTTCTCT TCACAGCAAA CAGCTTCGAC TCTATAAACG AGGATGCGTT CCTCGGTCTC 
CCTCATCTGG AGTATCTATT CATTGAAAAC AACCAGATCA AGTCCATATC GCCGTTCGCC 
TTCAGAGGAC TCAAGTCTTT AATTCACCTA AGTCTGGCGT ACAATAATCT GGAGACGCTG 
CCCAAAGACC TCTTCAAGGG GATGGAAGCT TTGACTAAAG TGGATCTGAG AGGGAATCTG 
TTCAGCTGTG ACTGTAAGCT GAAGTGGTTG GTGGACTGGA TGTTTCACAC AAACGCTACA 
GTGGATCAGA TCTTCTGCAA CGGCCCTGAA GGGTACCAGG GGAAAAAAAT CAACGATCTG 
GAGGCGCAGA CCTTCGACTG CATCACCACA GATTTTACCC TGCTGAAGTC TCTGGAGTTT 
CAGTCCATCT CAGTGGAGGC TTTTTCCTTC GCTGGTGATC AGTTCGTAGT TTTCGCTCAG 
CCCTTCAGCG GCAGATGCAG CTTCATGGAG TGGGATCACG TGCAGATGGA GTTCAGAAAC 
TTCGACAACA TCACGAGCAC ATCCACGGTC ATCTGCAAAC CTCTAGTCAT CGACAGCCAG 
CTCTTCATCA TAGTAGCTCA GCTGTTCGGC GGCTCGCACA TCTTCAAGCG CGACGTCTCC 
GCAAACAAAT TCATCAGAAT CCAAGACATC GACATCCTGA AGATCCGCAA GCCCAACGAC 
GTGGAAATCT TCGAGGTAGA CGGAGAGTCC TTCTTCATCA TCGCCGACAG CTCGAAAGCT 
GGCTCCACCA CCATCTATAA GTGGAACGGA AACGGGTTTT ACTCCTACCA GTCGCTCCAT 
CCTTGGCACC GCGACACCGA TGTGGAGTAT CTGGACATTT CTGGAAAACC GCATCTGATT 
TTATCCAGCA GTTCGCAGAG GCCTGTTGTT TACCAGTGGA GCCGGGGCAG CGCTCAGTTC 
GAGAGGCGCA CTGACATCCC GGAGATGGAG GATGTGTTCG CGGTCAAACA TTTTACGGTG 
AAAAACGAGC TGTACATCTG CCTGACGCGC TTCATCGGCG ACTCCAAAGT GATGCGGTGG 
GATGGCAGCA TGTTTACAGA GATCCAGACC GTGGCTTCCC GGGGCTCCAT GGTGTTCCAG 
CCGTTCTCCG TGGCCAGCTG GCAGTACGCC ATCCTGGGCA GCGATTACGC CTTCACACGG 
GTCTACCGCT GGGACGCCAA GAAGAGGCAG TTCGTTCCCT TCCAGGAGCT GAACATCCAG 
GCGCCCAGGG CTTTCTCTCT GGTGTTCATC GATAACAGGG AGTTCCTGCT GGGGTCCAGT 
TTTAAGGGGC AAACGCGGAT ATATGAGCAT CTGGTGCTGG ACCTGAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955921.2
CDS	169..1782
Translation	MMCLRRAAVLCVLLAALVLGGRRRSSRCPAPCTCSKDNALCANTGAIPRSFPQDVISLSFVKSGFTEIPKESFIHTPALHLLLFTANSFDSINEDAFLGLPHLEYLFIENNQIKSISPFAFRGLKSLIHLSLAYNNLETLPKDLFKGMEALTKVDLRGNLFSCDCKLKWLVDWMFHTNATVDQIFCNGPEGYQGKKINDLEAQTFDCITTDFTLLKSLEFQSISVEAFSFAGDQFVVFAQPFSGRCSFMEWDHVQMEFRNFDNITSTSTVICKPLVIDSQLFIIVAQLFGGSHIFKRDVSANKFIRIQDIDILKIRKPNDVEIFEVDGESFFIIADSSKAGSTTIYKWNGNGFYSYQSLHPWHRDTDVEYLDISGKPHLILSSSSQRPVVYQWSRGSAQFERRTDIPEMEDVFAVKHFTVKNELYICLTRFIGDSKVMRWDGSMFTEIQTVASRGSMVFQPFSVASWQYAILGSDYAFTRVYRWDAKKRQFVPFQELNIQAPRAFSLVFIDNREFLLGSSFKGQTRIYEHLVLDLSS

Target ORF information:

RefSeq Version	NM_199627.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich, glioma inactivated 1a (lgi1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199627.2

ORF Insert Sequence:

ATGATGTGTT TGCGGCGGGC GGCGGTGCTG TGCGTGCTGC TGGCGGCGCT GGTGCTCGGC 
GGCCGGAGGA GGAGCTCCCG CTGCCCCGCG CCCTGCACCT GCAGCAAGGA TAACGCGTTA 
TGCGCCAATA CCGGAGCCAT ACCGAGGAGC TTCCCGCAGG ACGTCATCTC ACTATCATTC 
GTCAAATCCG GCTTTACTGA GATCCCCAAA GAGAGTTTCA TTCACACACC TGCACTACAC 
CTGCTTCTCT TCACAGCAAA CAGCTTCGAC TCTATAAACG AGGATGCGTT CCTCGGTCTC 
CCTCATCTGG AGTATCTATT CATTGAAAAC AACCAGATCA AGTCCATATC GCCGTTCGCC 
TTCAGAGGAC TCAAGTCTTT AATTCACCTA AGTCTGGCGT ACAATAATCT GGAGACGCTG 
CCCAAAGACC TCTTCAAGGG GATGGAAGCT TTGACTAAAG TGGATCTGAG AGGGAATCTG 
TTCAGCTGTG ACTGTAAGCT GAAGTGGTTG GTGGACTGGA TGTTTCACAC AAACGCTACA 
GTGGATCAGA TCTTCTGCAA CGGCCCTGAA GGGTACCAGG GGAAAAAAAT CAACGATCTG 
GAGGCGCAGA CCTTCGACTG CATCACCACA GATTTTACCC TGCTGAAGTC TCTGGAGTTT 
CAGTCCATCT CAGTGGAGGC TTTTTCCTTC GCTGGTGATC AGTTCGTAGT TTTCGCTCAG 
CCCTTCAGCG GCAGATGCAG CTTCATGGAG TGGGATCACG TGCAGATGGA GTTCAGAAAC 
TTCGACAACA TCACGAGCAC ATCCACGGTC ATCTGCAAAC CTCTAGTCAT CGACAGCCAG 
CTCTTCATCA TAGTAGCTCA GCTGTTCGGC GGCTCGCACA TCTTCAAGCG CGACGTCTCC 
GCAAACAAAT TCATCAGAAT CCAAGACATC GACATCCTGA AGATCCGCAA GCCCAACGAC 
GTGGAAATCT TCGAGGTAGA CGGAGAGTCC TTCTTCATCA TCGCCGACAG CTCGAAAGCT 
GGCTCCACCA CCATCTATAA GTGGAACGGA AACGGGTTTT ACTCCTACCA GTCGCTCCAT 
CCTTGGCACC GCGACACCGA TGTGGAGTAT CTGGACATTT CTGGAAAACC GCATCTGATT 
TTATCCAGCA GTTCGCAGAG GCCTGTTGTT TACCAGTGGA GCCGGGGCAG CGCTCAGTTC 
GAGAGGCGCA CTGACATCCC GGAGATGGAG GATGTGTTCG CGGTCAAACA TTTTACGGTG 
AAAAACGAGC TGTACATCTG CCTGACGCGC TTCATCGGCG ACTCCAAAGT GATGCGGTGG 
GATGGCAGCA TGTTTACAGA GATCCAGACC GTGGCTTCCC GGGGCTCCAT GGTGTTCCAG 
CCGTTCTCCG TGGCCAGCTG GCAGTACGCC ATCCTGGGCA GCGATTACGC CTTCACACGG 
GTCTACCGCT GGGACGCCAA GAAGAGGCAG TTCGTTCCCT TCCAGGAGCT GAACATCCAG 
GCGCCCAGGG CTTTCTCTCT GGTGTTCATC GATAACAGGG AGTTCCTGCT GGGGTCCAGT 
TTTAAGGGGC AAACGCGGAT ATATGAGCAT CTGGTGCTGG ACCTGAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Functional phylogenetic analysis of LGI proteins identifies an interaction motif crucial for myelination.
Development (Cambridge, England)141(8)1749-56(2014 Apr)
Kegel L,Jaegle M,Driegen S,Aunin E,Leslie K,Fukata Y,Watanabe M,Fukata M,Meijer D

Loss of zebrafish lgi1b leads to hydrocephalus and sensitization to pentylenetetrazol induced seizure-like behavior.
PloS one6(9)e24596(2011)
Teng Y,Xie X,Walker S,Saxena M,Kozlowski DJ,Mumm JS,Cowell JK