nup50 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup50
Entrez Gene ID	322969
Full Name	nucleoporin 50
Synonyms	wu:fa56e03,wu:fb78a08
General protein information	Preferred Names nucleoporin 50 Names nuclear pore complex protein Nup50 nucleoporin 50kDa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301036.2	XP_009299311.1	nuclear pore complex protein Nup50 isoform X1
NM_201580.2	NP_963874.2	nuclear pore complex protein Nup50

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nup50	NP_057923.2
Gallus gallus (chicken)	NUP50	NP_001012559.1
Danio rerio (zebrafish)	nup50	NP_963874.2
Pan troglodytes (chimpanzee)	NUP50	XP_001135300.1
	NUP50	XP_001109545.1
Bos taurus (cattle)	NUP50	NP_001033137.1
Xenopus tropicalis (tropical clawed frog)	nup50	NP_001016628.1
Canis lupus familiaris (dog)	NUP50	XP_531700.3
Rattus norvegicus (Norway rat)	Npap60	NP_037123.1
Homo sapiens (human)	NUP50	NP_009103.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nup50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30970	NM_201580.2 Latest version!	Danio rerio nucleoporin 50 (nup50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa09901	XM_009301036.2 Latest version!	Danio rerio nucleoporin 50 (nup50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup50 ( NM_201580.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_963874.2 Danio rerio nucleoporin 50 (nup50), mRNA.

Price & Availability↑

CloneID	ODa30970
Clone ID Related Accession (Same CDS sequence)	NM_201580.2
Accession Version	NM_201580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nuclear pore complex protein Nup50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065637.1. On Feb 9, 2004 this sequence version replaced NM_201580.1. ##Evidence-Data-START## Transcript exon combination :: BC065637.1, BC051604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGC GGATTGCGGA AAAAGAGTTG ACCGACAGGA ACTGGGATCA GGAGGATGAT 
GGAGAAGAGG CAGGAACGTT TTCGATTGCG AGTGATGATG TGATGAAAAA CCGCGCCATC 
AAGAAAGCCA AGCGGCGTAA CGTTGCAGAG GGAGAGAGTG GAGGGGCTTT CAAAGCGTTT 
AAGGGGTTTT CTCTGGCTCC AGCCACAGCG ACGGCTTCGT TCTCTGGCTT CGGCAATGGT 
GCTGGCTTCA AACCCATGGC CAGCCTGACC AATGGCAGCG TGGCATCAGT GGCTCCGTCA 
TTCACAGGCT TCAACGTTCC TGCATCTGCT AAAACTAACA GTGGTCCTTT AACATTCAGC 
AGCTCCACCC CCTCCAAGCC CACAGACAGC GAGGTCACCT CCAACGGCTC CGCCCCCAGC 
AGTAAAGAGT ACAACCGGCA GCTCACAGCG CTGAACTGCT CGGTGCGCGA CTGGATCACC 
AAACACGTCA ACGACAACCC GCTGTGCGAC CTCAACCCCA TCTTCCGGGA CTACGAGCGT 
CACCTGGCCA GCATCGAGAG GAAATACGGC AGCGGGGCGG CAGACGAAAC ACCAGGGAAG 
CCGCTGGCAT CCTGTGCTGC GGCTCCGCCG ACCGTCTCCC TCAAAAACAA GGACGACAAG 
CCTCCAGCAG AAAAGCCAGC AGCTCCTCCG GGAGTCTCCT TTAATTTCGG AAAGAAGGTG 
GACAGCTCTG TGCTCGGGGC GCTGTCGTCA GGTGGAGCGC CGTCGTTCTC CTTCTCCTCT 
TCGTCTTCTT CTTCTGCTGT GTTTGGCTCA GCCGTCAGTA GCGTCAGTCC TGTGGTCAAC 
TCGGACAGCA ACAGCCAGAC TGCAGATGAG AACGGGGAGG ACTCTGAGGA GCCGCCGGTG 
CCTGTGGTGA AGGAGATCAA GGAGAAAGAC GCTTTCTACT CCAAAAAGTG TAAGCTGTTC 
TACAAGAAAG ACGGCGAGTT TAAGGAGAAG GGCGTCGGGA CGCTGCATCT GAAGATGACC 
ACCGAGAGCA AACTCCAGCT GCTAGTGCGC GCAGACACCA ACCTGGGGAA CATCCTGCTG 
AACATCATGG TGCCCTCCTC CATGCCCTGC TCACGAACTG GCAAAAACAA TGTGATGGTG 
GTGTGTGTGC CAAACCCTCC CGTGGACGAC AAGAACCCCA GTGTGCCCGT CCCAGTGCTG 
ATCCGCGTCA AAACAGCCGA GGACGCAGAC CAACTGCACC GAGTCCTCCA GGAGAAGAAA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963874.2
CDS	118..1383
Translation	MAKRIAEKELTDRNWDQEDDGEEAGTFSIASDDVMKNRAIKKAKRRNVAEGESGGAFKAFKGFSLAPATATASFSGFGNGAGFKPMASLTNGSVASVAPSFTGFNVPASAKTNSGPLTFSSSTPSKPTDSEVTSNGSAPSSKEYNRQLTALNCSVRDWITKHVNDNPLCDLNPIFRDYERHLASIERKYGSGAADETPGKPLASCAAAPPTVSLKNKDDKPPAEKPAAPPGVSFNFGKKVDSSVLGALSSGGAPSFSFSSSSSSSAVFGSAVSSVSPVVNSDSNSQTADENGEDSEEPPVPVVKEIKEKDAFYSKKCKLFYKKDGEFKEKGVGTLHLKMTTESKLQLLVRADTNLGNILLNIMVPSSMPCSRTGKNNVMVVCVPNPPVDDKNPSVPVPVLIRVKTAEDADQLHRVLQEKKA

Target ORF information:

RefSeq Version	NM_201580.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleoporin 50 (nup50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201580.2

ORF Insert Sequence:

ATGGCCAAGC GGATTGCGGA AAAAGAGTTG ACCGACAGGA ACTGGGATCA GGAGGATGAT 
GGAGAAGAGG CAGGAACGTT TTCGATTGCG AGTGATGATG TGATGAAAAA CCGCGCCATC 
AAGAAAGCCA AGCGGCGTAA CGTTGCAGAG GGAGAGAGTG GAGGGGCTTT CAAAGCGTTT 
AAGGGGTTTT CTCTGGCTCC AGCCACAGCG ACGGCTTCGT TCTCTGGCTT CGGCAATGGT 
GCTGGCTTCA AACCCATGGC CAGCCTGACC AATGGCAGCG TGGCATCAGT GGCTCCGTCA 
TTCACAGGCT TCAACGTTCC TGCATCTGCT AAAACTAACA GTGGTCCTTT AACATTCAGC 
AGCTCCACCC CCTCCAAGCC CACAGACAGC GAGGTCACCT CCAACGGCTC CGCCCCCAGC 
AGTAAAGAGT ACAACCGGCA GCTCACAGCG CTGAACTGCT CGGTGCGCGA CTGGATCACC 
AAACACGTCA ACGACAACCC GCTGTGCGAC CTCAACCCCA TCTTCCGGGA CTACGAGCGT 
CACCTGGCCA GCATCGAGAG GAAATACGGC AGCGGGGCGG CAGACGAAAC ACCAGGGAAG 
CCGCTGGCAT CCTGTGCTGC GGCTCCGCCG ACCGTCTCCC TCAAAAACAA GGACGACAAG 
CCTCCAGCAG AAAAGCCAGC AGCTCCTCCG GGAGTCTCCT TTAATTTCGG AAAGAAGGTG 
GACAGCTCTG TGCTCGGGGC GCTGTCGTCA GGTGGAGCGC CGTCGTTCTC CTTCTCCTCT 
TCGTCTTCTT CTTCTGCTGT GTTTGGCTCA GCCGTCAGTA GCGTCAGTCC TGTGGTCAAC 
TCGGACAGCA ACAGCCAGAC TGCAGATGAG AACGGGGAGG ACTCTGAGGA GCCGCCGGTG 
CCTGTGGTGA AGGAGATCAA GGAGAAAGAC GCTTTCTACT CCAAAAAGTG TAAGCTGTTC 
TACAAGAAAG ACGGCGAGTT TAAGGAGAAG GGCGTCGGGA CGCTGCATCT GAAGATGACC 
ACCGAGAGCA AACTCCAGCT GCTAGTGCGC GCAGACACCA ACCTGGGGAA CATCCTGCTG 
AACATCATGG TGCCCTCCTC CATGCCCTGC TCACGAACTG GCAAAAACAA TGTGATGGTG 
GTGTGTGTGC CAAACCCTCC CGTGGACGAC AAGAACCCCA GTGTGCCCGT CCCAGTGCTG 
ATCCGCGTCA AAACAGCCGA GGACGCAGAC CAACTGCACC GAGTCCTCCA GGAGAAGAAA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup50 ( XM_009301036.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299311.1 Danio rerio nucleoporin 50 (nup50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09901
Clone ID Related Accession (Same CDS sequence)	XM_009301036.2
Accession Version	XM_009301036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear pore complex protein Nup50 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GGATTGCGGA AAAAGAGTTG ACCGACAGGA ACTGGGATCA GGAGGATGAT 
GGAGAAGAGG CAGGAACGTT TTCGATTGCG AGTGATGATG TGATGAAAAA CCGCGCCATC 
AAGAAAGCCA AGCGGCGTAA CGTTGCAGAG GGAGAGAGTG GAGGGGCTTT CAAAGCGTTT 
AAGGGGTTTT CTCTGGCTCC AGCCACAGCG ACGGCTTCGT TCTCTGGCTT CGGCAATGGT 
GCTGGCTTCA AACCCATGGC CAGCCTGACC AATGGCAGCG TGGCATCAGT GGCTCCGTCA 
TTCACAGGTT TCAACGTTCC TGCATCTGCT AAAACTAACA GTGGTCCTTT AACATTCAGC 
AGCTCCACCC CCTCCAAGCC CACAGACGGT GAGGTCACCT CCAACGGCTC CGCCCCCAGC 
AGTAAAGAGT ACAACCGGCA GCTCACAGCG CTGAACTGCT CGGTGCGCGA CTGGATCACC 
AAACACGTCA ACGACAACCC GCTGTGCGAC CTCAACCCCA TCTTCCGGGA CTACGAGCGT 
CACCTGGCCA GCATCGAGAG GAAATACGGC AGCGGGGCGG CAGACGAAAC ACCAGGGAAG 
CCGCTGGCAT CCTGTGCTGC GGCTCCGCAG ACCGTCTCCC TCAAAAACAA GGACGACAAG 
CCTCCAGCAG AAAAGCCAGC AGCTCCTCCG GGAGTCTCCT TTAATTTCGG AAAGAAGGTG 
GACAGCTCGG TGCTCGGGGC GCTGTCGTCA GGTGGAGCGC CGTCGTTTTC CTTCTCCTCT 
TCGTCTTCTT CTTCTGCTGT GTTTGGCTCA GCCGTCAGTA GCGTCAGTAG TCCTGTGGTC 
AACTCGGACA GCAACAGCCA GACTGCAGAT GAGAACGGGG AGGACTCTGA GGAGCCGCCG 
GTGCCTGTGG TGAAGGAGAT CAAGGAGAAA GACGCTTTCT ACTCCAAAAA GTGTAAGCTG 
TTCTACAAGA AAGACGGCGA GTTTAAGGAG AAGGGCGTCG GGACGCTGCA TCTGAAGATG 
ACCACCGAGA GCAAACTCCA GCTGCTAGTG CGCGCAGACA CCAACCTGGG GAACATCCTG 
CTGAACATCA TGGTGCCCTC CTCCATGCCC TGCTCACGAA CTGGCAAAAA CAATGTGATG 
GTGGTGTGTG TGCCAAACCC TCCCGTGGAC GACAAGAACC CCAGCGTGCC CGTCCCAGTG 
CTGATCCGCG TCAAAACAGC CGAGGACGCA GACCAACTGC ACCGAGTCCT CCAGGAGAAG 
AAAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299311.1
CDS	83..1351
Translation	MAKRIAEKELTDRNWDQEDDGEEAGTFSIASDDVMKNRAIKKAKRRNVAEGESGGAFKAFKGFSLAPATATASFSGFGNGAGFKPMASLTNGSVASVAPSFTGFNVPASAKTNSGPLTFSSSTPSKPTDGEVTSNGSAPSSKEYNRQLTALNCSVRDWITKHVNDNPLCDLNPIFRDYERHLASIERKYGSGAADETPGKPLASCAAAPQTVSLKNKDDKPPAEKPAAPPGVSFNFGKKVDSSVLGALSSGGAPSFSFSSSSSSSAVFGSAVSSVSSPVVNSDSNSQTADENGEDSEEPPVPVVKEIKEKDAFYSKKCKLFYKKDGEFKEKGVGTLHLKMTTESKLQLLVRADTNLGNILLNIMVPSSMPCSRTGKNNVMVVCVPNPPVDDKNPSVPVPVLIRVKTAEDADQLHRVLQEKKA

Target ORF information:

RefSeq Version	XM_009301036.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleoporin 50 (nup50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301036.2

ORF Insert Sequence:

ATGGCCAAGC GGATTGCGGA AAAAGAGTTG ACCGACAGGA ACTGGGATCA GGAGGATGAT 
GGAGAAGAGG CAGGAACGTT TTCGATTGCG AGTGATGATG TGATGAAAAA CCGCGCCATC 
AAGAAAGCCA AGCGGCGTAA CGTTGCAGAG GGAGAGAGTG GAGGGGCTTT CAAAGCGTTT 
AAGGGGTTTT CTCTGGCTCC AGCCACAGCG ACGGCTTCGT TCTCTGGCTT CGGCAATGGT 
GCTGGCTTCA AACCCATGGC CAGCCTGACC AATGGCAGCG TGGCATCAGT GGCTCCGTCA 
TTCACAGGTT TCAACGTTCC TGCATCTGCT AAAACTAACA GTGGTCCTTT AACATTCAGC 
AGCTCCACCC CCTCCAAGCC CACAGACGGT GAGGTCACCT CCAACGGCTC CGCCCCCAGC 
AGTAAAGAGT ACAACCGGCA GCTCACAGCG CTGAACTGCT CGGTGCGCGA CTGGATCACC 
AAACACGTCA ACGACAACCC GCTGTGCGAC CTCAACCCCA TCTTCCGGGA CTACGAGCGT 
CACCTGGCCA GCATCGAGAG GAAATACGGC AGCGGGGCGG CAGACGAAAC ACCAGGGAAG 
CCGCTGGCAT CCTGTGCTGC GGCTCCGCAG ACCGTCTCCC TCAAAAACAA GGACGACAAG 
CCTCCAGCAG AAAAGCCAGC AGCTCCTCCG GGAGTCTCCT TTAATTTCGG AAAGAAGGTG 
GACAGCTCGG TGCTCGGGGC GCTGTCGTCA GGTGGAGCGC CGTCGTTTTC CTTCTCCTCT 
TCGTCTTCTT CTTCTGCTGT GTTTGGCTCA GCCGTCAGTA GCGTCAGTAG TCCTGTGGTC 
AACTCGGACA GCAACAGCCA GACTGCAGAT GAGAACGGGG AGGACTCTGA GGAGCCGCCG 
GTGCCTGTGG TGAAGGAGAT CAAGGAGAAA GACGCTTTCT ACTCCAAAAA GTGTAAGCTG 
TTCTACAAGA AAGACGGCGA GTTTAAGGAG AAGGGCGTCG GGACGCTGCA TCTGAAGATG 
ACCACCGAGA GCAAACTCCA GCTGCTAGTG CGCGCAGACA CCAACCTGGG GAACATCCTG 
CTGAACATCA TGGTGCCCTC CTCCATGCCC TGCTCACGAA CTGGCAAAAA CAATGTGATG 
GTGGTGTGTG TGCCAAACCC TCCCGTGGAC GACAAGAACC CCAGCGTGCC CGTCCCAGTG 
CTGATCCGCG TCAAAACAGC CGAGGACGCA GACCAACTGC ACCGAGTCCT CCAGGAGAAG 
AAAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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nup50 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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