rad21a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad21a
Entrez Gene ID	322275
Full Name	RAD21 cohesin complex component a
Synonyms	rad21,wu:fb55d02,wu:fb93f04
General protein information	Preferred Names RAD21 cohesin complex component a Names RAD21 cohesin complex component a RAD21 homolog a double-strand-break repair protein rad21 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199595.1	NP_955889.1	double-strand-break repair protein rad21 homolog A

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD21	NP_006256.1
	RAD21	XP_001095142.1
Bos taurus (cattle)	RAD21	NP_001029889.1
Drosophila melanogaster (fruit fly)	vtd	NP_001015132.1
Canis lupus familiaris (dog)	RAD21	XP_539142.2
Mus musculus (house mouse)	Rad21	NP_033035.3
Gallus gallus (chicken)	RAD21	NP_001026121.1
Danio rerio (zebrafish)	LOC100536176	XP_003200317.1
Rattus norvegicus (Norway rat)	Rad21	NP_001020872.1
Danio rerio (zebrafish)	rad21b	NP_001038585.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004560	XP_313862.4
Xenopus tropicalis (tropical clawed frog)	rad21	XP_002940047.1
Pan troglodytes (chimpanzee)	RAD21	XP_003951384.1
Danio rerio (zebrafish)	rad21a	NP_955889.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A neural crest origin for cohesinopathy heart defects.
Schuster K, Leeke B, Meier M, Wang Y, Newman T, Burgess S, Horsfield JA
Human molecular genetics24(24)7005-16(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction.
Bonora E, Bianco F, Cordeddu L, Bamshad M, Francescatto L, Dowless D, Stanghellini V, Cogliandro RF, Lindberg G, Mungan Z, Cefle K, Ozcelik T, Palanduz S, Ozturk S, Gedikbasi A, Gori A, Pippucci T, Graziano C, Volta U, Caio G, Barbara G, D'Amato M, Seri M, Katsanis N, Romeo G, De Giorgio R
Gastroenterology148(4)771-782.e11(2015 Apr)

L-leucine partially rescues translational and developmental defects associated with zebrafish models of Cornelia de Lange syndrome.
Xu B, Sowa N, Cardenas ME, Gerton JL
Human molecular genetics24(6)1540-55(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The hearts of Rad21-depleted animals were smaller, often failed to loop, and functioned less efficiently than size-matched controls
Title: A neural crest origin for cohesinopathy heart defects.

RAD21a suppression led to delayed intestinal transit and greatly reduced numbers of enteric neurons, similar to patients with chronic intestinal pseudo-obstruction.
Title: Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction.

Cohesin is a protein complex essential for sister chromatid cohesion and DNA repair that also appears to be essential for normal development through as yet unknown mechanisms.
Title: Cohesin-dependent regulation of Runx genes.

The following rad21a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad21a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36755	NM_199595.1 Latest version!	Danio rerio RAD21 cohesin complex component a (rad21a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad21a ( NM_199595.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955889.1 Danio rerio RAD21 cohesin complex component a (rad21a), mRNA.

Price & Availability↑

CloneID	ODa36755
Clone ID Related Accession (Same CDS sequence)	NM_199595.1
Accession Version	NM_199595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Danio rerio(zebrafish)
Product	double-strand-break repair protein rad21 homolog A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045311.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045311.1, AY423040.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTTACG CCCACTTCGT CCTCAGTAAA CGTGGGCCGC TAGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAAAAGCT GACCAAAGCG CACGTCTTTG AATGCAATCT AGAGAGCAGC 
GTGGAAAGCA TCATTTCCCC AAAGGTGAAA ATGGCTCTGC GTACATCTGG TCATCTTCTC 
CTGGGTGTGG TGAGGATCTA CCACAGAAAA GCCAAATATC TTCTGGCTGA TTGTAATGAG 
GCATTTATCA AGATCAAAAT GGCTTTCCGC CCAGGTGTCG TTGATCTGCC AGAAGACAAC 
CGTGAGGCAG CATACAATGC CATCACCTTG CCAGAGGAGT TCCACGATTT TGACCAGCCT 
CTTCCTGATC TTGATGATAT TGATGTAGCA CAGCAGTTCA CCCTGAATCA AAGTCGTGTT 
GAAGAGATCA CCATGAGGGA GGAAGTAGGA AACATCAGCC TCATGACTGA CAACGACTTT 
GGTGACTTCG GCATGGATGA TCGCGAGATG ATGAGGGAGG AGAATGCTTT TGGAGACATC 
ATCCATGAAT CGTCTGCTGC CAATCTTCTT TTGGAAGCTG AACCTGGTCC TGCTCACCTC 
CCCGACAAAA CACCCAACCT TGACTATGAT GACTTTGGAG ACAACAACCT GGAGAACAGC 
GATGGCGGAA TTTTAATGGA CAAGATCCTG AGCAATGAGG ATGGCGGTGG TATATTTGAT 
GATCCTCCAG CTATCACTGA CAGTGTGATG ATGCCTCAAG ACCATGGAGA TGATGATGAC 
GACTTTGATA ACTTTTCTCC AGCTGGAGGT CCAGACAGTC CAGACTCTGG ACCCGTGGAG 
CCTCTTCCCA ACACCACTGA CCAGACCGAT CAGACCGAAC AGACCACTCT TGTGCCCAAT 
GAGGATGAAG CTTTCGCTCT TGAGCCCATC GACATCACTG TAAAAGAGAC CAAGGCTAAG 
AGAAAGAGGA AGCTCATCGT CGACAGCCTG AAGGAGTTGG ACAGCAAGAC CATCCGTGCC 
CAGCTGAGTG ATTACTCTGA TATCGTCACC ACGCTGGATT TGGCTCCTCC AACCAAAAAG 
CTGATGATGT GGAAGGAGAC TGGAGGTGTG GAGAAGCTCT TCTCATTGCC TGCTCAGCCT 
CTCTGGAACG GCCGTCTGCT TAAGATGTTT ACTCGCTGCT TGACTCCACT GGTGCCTGAT 
GAGCTGAGGA AGAGGAGGAA GGGAGGAGAA GCCGACAGCC TTGAGGACTT CCTGAAGGAG 
CTGGAGAACC CAGAAGTGCC CAGAGAGGAG GTGCTGGGTC ACAGGAGCGA CGTTATCGAT 
CAGACCATCA TGGAGGAGCC CAGTATGCTG CAGGCGTCCT CTATGGAGGG CAGCAGAACC 
GCTCTCGATG AGTCCATGAT GCCTCCTCCA TCCCGCCAGC GCGGACTCAA GCGCAAGTCA 
CTCGAGACGC TCGAGAAAGA TGCAGTTCTG CCTCCAATGG GAGTGTTGGA GCAGACACTT 
CAGCCCTTGG ATCAGTCTGT GCTTTCCCAT CAGCTCGAGA TGCCGCAGGT GGAGCTTCCT 
CCTGAGGACA CCACTAACCT CTCTAGACTC ATTCCAGAGC TGGATCTGTT GGATGAAAAG 
AGCAAGGATA AAGACAAGGA CGACAGCGAC GAAGAGGGTG AGGAAGGACA GGGAGGAGAT 
CAGGACCAAG AGGAGAGAAG GTGGAACAAG AGAACTCAGC AGATGCTTCA CGGTCTTCAG 
CGCGTGGTGG CTAAAACCGG AGCCCAGTCC ATCGGCCTGC TGGAGCTGTG CAGAAACAAC 
AACAAGAAAC AGGCTGCGGC CAAGTTTTAC AGCTTCCTGG TGCTGAAGAA GCAGCAGGCC 
ATCGACCTGA CGCAGACCGA GCCCTACAGT GACATCATCG CCGCGCCGGG ACCACGCTTC 
CACATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955889.1
CDS	146..2077
Translation	MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEDNREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFTLNQSRVEEITMREEVGNISLMTDNDFGDFGMDDREMMREENAFGDIIHESSAANLLLEAEPGPAHLPDKTPNLDYDDFGDNNLENSDGGILMDKILSNEDGGGIFDDPPAITDSVMMPQDHGDDDDDFDNFSPAGGPDSPDSGPVEPLPNTTDQTDQTEQTTLVPNEDEAFALEPIDITVKETKAKRKRKLIVDSLKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFSLPAQPLWNGRLLKMFTRCLTPLVPDELRKRRKGGEADSLEDFLKELENPEVPREEVLGHRSDVIDQTIMEEPSMLQASSMEGSRTALDESMMPPPSRQRGLKRKSLETLEKDAVLPPMGVLEQTLQPLDQSVLSHQLEMPQVELPPEDTTNLSRLIPELDLLDEKSKDKDKDDSDEEGEEGQGGDQDQEERRWNKRTQQMLHGLQRVVAKTGAQSIGLLELCRNNNKKQAAAKFYSFLVLKKQQAIDLTQTEPYSDIIAAPGPRFHIV

Target ORF information:

RefSeq Version	NM_199595.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAD21 cohesin complex component a (rad21a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199595.1

ORF Insert Sequence:

ATGTTTTACG CCCACTTCGT CCTCAGTAAA CGTGGGCCGC TAGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAAAAGCT GACCAAAGCG CACGTCTTTG AATGCAATCT AGAGAGCAGC 
GTGGAAAGCA TCATTTCCCC AAAGGTGAAA ATGGCTCTGC GTACATCTGG TCATCTTCTC 
CTGGGTGTGG TGAGGATCTA CCACAGAAAA GCCAAATATC TTCTGGCTGA TTGTAATGAG 
GCATTTATCA AGATCAAAAT GGCTTTCCGC CCAGGTGTCG TTGATCTGCC AGAAGACAAC 
CGTGAGGCAG CATACAATGC CATCACCTTG CCAGAGGAGT TCCACGATTT TGACCAGCCT 
CTTCCTGATC TTGATGATAT TGATGTAGCA CAGCAGTTCA CCCTGAATCA AAGTCGTGTT 
GAAGAGATCA CCATGAGGGA GGAAGTAGGA AACATCAGCC TCATGACTGA CAACGACTTT 
GGTGACTTCG GCATGGATGA TCGCGAGATG ATGAGGGAGG AGAATGCTTT TGGAGACATC 
ATCCATGAAT CGTCTGCTGC CAATCTTCTT TTGGAAGCTG AACCTGGTCC TGCTCACCTC 
CCCGACAAAA CACCCAACCT TGACTATGAT GACTTTGGAG ACAACAACCT GGAGAACAGC 
GATGGCGGAA TTTTAATGGA CAAGATCCTG AGCAATGAGG ATGGCGGTGG TATATTTGAT 
GATCCTCCAG CTATCACTGA CAGTGTGATG ATGCCTCAAG ACCATGGAGA TGATGATGAC 
GACTTTGATA ACTTTTCTCC AGCTGGAGGT CCAGACAGTC CAGACTCTGG ACCCGTGGAG 
CCTCTTCCCA ACACCACTGA CCAGACCGAT CAGACCGAAC AGACCACTCT TGTGCCCAAT 
GAGGATGAAG CTTTCGCTCT TGAGCCCATC GACATCACTG TAAAAGAGAC CAAGGCTAAG 
AGAAAGAGGA AGCTCATCGT CGACAGCCTG AAGGAGTTGG ACAGCAAGAC CATCCGTGCC 
CAGCTGAGTG ATTACTCTGA TATCGTCACC ACGCTGGATT TGGCTCCTCC AACCAAAAAG 
CTGATGATGT GGAAGGAGAC TGGAGGTGTG GAGAAGCTCT TCTCATTGCC TGCTCAGCCT 
CTCTGGAACG GCCGTCTGCT TAAGATGTTT ACTCGCTGCT TGACTCCACT GGTGCCTGAT 
GAGCTGAGGA AGAGGAGGAA GGGAGGAGAA GCCGACAGCC TTGAGGACTT CCTGAAGGAG 
CTGGAGAACC CAGAAGTGCC CAGAGAGGAG GTGCTGGGTC ACAGGAGCGA CGTTATCGAT 
CAGACCATCA TGGAGGAGCC CAGTATGCTG CAGGCGTCCT CTATGGAGGG CAGCAGAACC 
GCTCTCGATG AGTCCATGAT GCCTCCTCCA TCCCGCCAGC GCGGACTCAA GCGCAAGTCA 
CTCGAGACGC TCGAGAAAGA TGCAGTTCTG CCTCCAATGG GAGTGTTGGA GCAGACACTT 
CAGCCCTTGG ATCAGTCTGT GCTTTCCCAT CAGCTCGAGA TGCCGCAGGT GGAGCTTCCT 
CCTGAGGACA CCACTAACCT CTCTAGACTC ATTCCAGAGC TGGATCTGTT GGATGAAAAG 
AGCAAGGATA AAGACAAGGA CGACAGCGAC GAAGAGGGTG AGGAAGGACA GGGAGGAGAT 
CAGGACCAAG AGGAGAGAAG GTGGAACAAG AGAACTCAGC AGATGCTTCA CGGTCTTCAG 
CGCGTGGTGG CTAAAACCGG AGCCCAGTCC ATCGGCCTGC TGGAGCTGTG CAGAAACAAC 
AACAAGAAAC AGGCTGCGGC CAAGTTTTAC AGCTTCCTGG TGCTGAAGAA GCAGCAGGCC 
ATCGACCTGA CGCAGACCGA GCCCTACAGT GACATCATCG CCGCGCCGGG ACCACGCTTC 
CACATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A neural crest origin for cohesinopathy heart defects.
Human molecular genetics24(24)7005-16(2015 Dec)
Schuster K,Leeke B,Meier M,Wang Y,Newman T,Burgess S,Horsfield JA

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction.
Gastroenterology148(4)771-782.e11(2015 Apr)
Bonora E,Bianco F,Cordeddu L,Bamshad M,Francescatto L,Dowless D,Stanghellini V,Cogliandro RF,Lindberg G,Mungan Z,Cefle K,Ozcelik T,Palanduz S,Ozturk S,Gedikbasi A,Gori A,Pippucci T,Graziano C,Volta U,Caio G,Barbara G,D'Amato M,Seri M,Katsanis N,Romeo G,De Giorgio R

L-leucine partially rescues translational and developmental defects associated with zebrafish models of Cornelia de Lange syndrome.
Human molecular genetics24(6)1540-55(2015 Mar)
Xu B,Sowa N,Cardenas ME,Gerton JL

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rad21a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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