wdr36 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wdr36
Entrez Gene ID	321745
Full Name	WD repeat domain 36
Synonyms	wu:fb33e10,zgc:66324
General protein information	Preferred Names WD repeat domain 36 Names WD repeat-containing protein 36
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308831.1	NP_001295760.1	WD repeat-containing protein 36

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UTP21	NP_013513.3
Magnaporthe oryzae (rice blast fungus)	MGG_16744	XP_003711887.1
Xenopus tropicalis (tropical clawed frog)	wdr36	NP_001120392.1
Danio rerio (zebrafish)	wdr36	NP_955860.1
Eremothecium gossypii	AGOS_AER081C	NP_984941.2
Pan troglodytes (chimpanzee)	WDR36	XP_517870.2
Mus musculus (house mouse)	Wdr36	NP_001103485.1
Rattus norvegicus (Norway rat)	LOC688637	XP_006254674.1
Gallus gallus (chicken)	WDR36	NP_001038099.1
Kluyveromyces lactis	KLLA0B02827g	XP_451658.1
Schizosaccharomyces pombe (fission yeast)	SPCC1672.07	NP_587878.1
Homo sapiens (human)	WDR36	NP_644810.1
Drosophila melanogaster (fruit fly)	CG9799	NP_650284.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004557	XP_313855.3
Neurospora crassa	NCU07011	XP_961832.1
Arabidopsis thaliana (thale cress)	AT4G04940	NP_567275.1
Canis lupus familiaris (dog)	WDR36	XP_545992.2
Bos taurus (cattle)	WDR36	XP_002691114.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The primary open-angle glaucoma gene WDR36 functions in ribosomal RNA processing and interacts with the p53 stress-response pathway.
Skarie JM, Link BA
Human molecular genetics17(16)2474-85(2008 Aug)

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Wdr36 function leads to an activation of the p53 stress-response pathway, suggesting that defects in p53 pathway may influence the impact of WDR36 variants on primary open-angle glaucoma.
Title: The primary open-angle glaucoma gene WDR36 functions in ribosomal RNA processing and interacts with the p53 stress-response pathway.

The following wdr36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wdr36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39681	NM_001308831.1 Latest version!	Danio rerio WD repeat domain 36 (wdr36), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wdr36 ( NM_001308831.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001295760.1 Danio rerio WD repeat domain 36 (wdr36), mRNA.

Price & Availability↑

CloneID	ODa39681
Clone ID Related Accession (Same CDS sequence)	NM_001308831.1
Accession Version	NM_001308831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	WD repeat-containing protein 36
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX294395.15. On Jan 27, 2016 this sequence version replaced NM_199566.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY648830.1, GFIL01043298.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAGCA TGTCTGCGAA CAAAAAAGGG AGCGTGATCT TCTCTGGATT CAGGGCGCTG 
GGGCTTTACT CTAATCATCT GGCGCACGTG GTTCGCTTCC ATAAGAAACA CAGAGAGTTC 
TATGTGGTCA CTGCAGTCGG ACAATGTTTC CATACATATA ACGTAAAGAA ACTAGGAATC 
GTTGCAGTCA GTAATGCCCT CCCAGAAGAC ATTTCATCCG TGACAGCAGA TTGTATGCTT 
GTCTATGCAG CATATGGAAA ACTTATCTCC GCTTTTGCTA GAAGCAGAGA GGTTGTTCAC 
ACATACACCG GTCACCAAGC TGATGTGCAT TTGCTGCTGC CTTTTGGAAA TCATTTGATT 
TCTGTTGATA AGGATAATGT GGTTATCATT TGGGATGTGG AGTCTGAGGA CACATATCTG 
CAGATTTCAT ATGACAAAGC ATCTTTTGAG GTCTCCGCTT TGATGCATCC GAGCACCTAC 
CTGAACAAAA TCCTGTTTGG AAGCAGTCAA GGGAGTCTTC AGTTATGGAA CATCAAATCA 
AACAAACTCC TGTTCACATT TTCTGGATGG TCAGCTGCTG TTACTGTTCT TCAACAGACC 
CCAGCTGTGG ATGTTGTAGG TGTGGGTTTA GCATCTGGCC AGATCATCAT TCACAATATT 
AAATTTGACG AAACACTTAT GAAGTTCCAA CAAGACTGGG GTCCAATCAC AGCTTTATCC 
TTCAGGACAG ATGGACATCC AATAATGGCA TCTGGAAGTC CTATTGGACA CATAGGACTG 
TGGGATTTAG AGGAGAAGAA ACTAGTCAGT CAAATGCGAG ATGCTCATAC AACCGCAATC 
GCTGGCCTCA CGTTTCTTCA AAATGAACCT CTTTTGATCA CCAACGGAGC GGATAATGCA 
ATACGGGTGT GGATTTTTGA CGTTGCTGGT GGAGATGGTC GTCTGCTCAG ACAGAGGATG 
GGTCACAGCG CCCCACCAAC AAAAATTCGC CATTATGATC AAAGTGGCTT GAACATTCTT 
AGCGCAGGTC AAGATGGCAC TTTGCAGTCC TTTTCGACTG TTCATGAAAG GTTCAACAAG 
AGTTTGGGGC ATGGAACCCT CAACAAAGCA AAAGCTAAAA AGAAAGGCGT GAAGTATGAC 
ACAGCGAAAC TCCCATCCAT CACAACATTT GCTTCAGAAA CAGCACGTCA GAGTGACTGG 
GATGGCATTA TTGCATGCCA TCGTGGCTTT CTTATGACAA CATCATGGAA CTACCAGAAG 
GGTTCAATGG GTGCGCACAA ACTGGAGCCT GAACGATTCA ACAGGAACCG TAGCCTCAAT 
GTCCATGCAA CTGCTGTGGA TATCAGCTCT TGTGGGAACT TTGCTGTGAT TGCGCTGTCT 
TCGGGACACA TCGATGTGTA CAACATGCAG TCTGGTCTTC ATCGTGGGCA TTATGGAGAG 
GACAAGGCAC ATGAAGGGCC TGTGCGAGGA GTGGCTGTGG ATGCTTTAAA CCAGTTGACT 
TTTAGTGTGG GAGCAGACCA TCTTCTGAAA ATCTGGAAGT TCAAATCAAA AGAGCACTTA 
CATACTCACA GGTTACCTGC AGCCCCGGCA TCATCTCTGC TTCACAGGGA CAGTGGGATG 
CTCGCCATTG CTCTAGATGT TTTTGCCATT CACATTTTGG ACGTGGAAAC TAGGAGGATT 
GTCCGCAAAT TCTCTGGACA TCGTGGCCAG ATCAATGACA TGACATTTAG TCCAGATGGA 
CGTTGGCTGA TTACAGCATC TATGGACTGC ACCATCAGAA CATGGGATCT TCCATCTGGC 
AGTTTGGTCG ACTGTTTCCT GGTAGAAGCA GCAGCCGTGA GTGTCAGTTT GTCCCCTACC 
GGGAACTTCC TGGCCTCGTC GCACGTTGAC GGCCTCGGTG TTTACTTATG GTCCAATAAC 
ACGCTGTGCA GTTTGGTGTC ATTGCGCCCT CTACCTGCAG ACTATGAGCC GACTGTGATC 
ATGCTGCCTG GCAATTGCCC AAGCAAAGAT ACAGAAGAGG AGGAGGTCTT AGACTCTGAC 
AGTTCTGAGA TGGCAGAGTA CATTTCTCCA GCACAGTTAG ATGAACATCT GGTCACTCTG 
TCACTGCTTC CTGACTCACG CTGGAAGAAC CTGCTGCATC TCGACATCAT CAAGAAAAAG 
AACAAACCCA AAGAGCCTCC AAAAGTGCCC AAAGCAGCAC CCTTCTTTAT TCCCACCATT 
CCAGGGCTGG TGCCCCAGTT TTCTCTTCCC AACACACCTT CAGAAGCCCA GTCTAAAGTT 
GTCAACTTAG GAGTATTGGC ACAGAAGTCC AACTTCTACA TTGAGCTTGA GAATGCTATA 
GAGAGCAACT CCTATGAAGA GCCGGTAAAG CTGTTGAGAG AGTTGGGGCC CTCTGCCATT 
GAAACAGAAC TTCGGGCCCT GTCTCCAGAT ATGGGTGGAG ATGTTGGAGT CATGAAGAGC 
TTCCTCAAGA TGATCAGCTG CATCCTTAAG TCCAAAAAAG ATTTTGACCT GGCCCAAGCC 
TATCTGGCAT TATTCCTAAA GCTGCATCTT CGGTTGATCT CGGATGAGCC TGAACTGATG 
GAGGAGGCAG AGAGAGTGTC TGCGCAGCTG GAGGAGACGT GGATGTGCAT ACAGACGCTC 
TTAAACCAGA GCATTTGTCT GTTGTCTTAT ATCAAGAGTG CGCTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295760.1
CDS	150..2840
Translation	MSSMSANKKGSVIFSGFRALGLYSNHLAHVVRFHKKHREFYVVTAVGQCFHTYNVKKLGIVAVSNALPEDISSVTADCMLVYAAYGKLISAFARSREVVHTYTGHQADVHLLLPFGNHLISVDKDNVVIIWDVESEDTYLQISYDKASFEVSALMHPSTYLNKILFGSSQGSLQLWNIKSNKLLFTFSGWSAAVTVLQQTPAVDVVGVGLASGQIIIHNIKFDETLMKFQQDWGPITALSFRTDGHPIMASGSPIGHIGLWDLEEKKLVSQMRDAHTTAIAGLTFLQNEPLLITNGADNAIRVWIFDVAGGDGRLLRQRMGHSAPPTKIRHYDQSGLNILSAGQDGTLQSFSTVHERFNKSLGHGTLNKAKAKKKGVKYDTAKLPSITTFASETARQSDWDGIIACHRGFLMTTSWNYQKGSMGAHKLEPERFNRNRSLNVHATAVDISSCGNFAVIALSSGHIDVYNMQSGLHRGHYGEDKAHEGPVRGVAVDALNQLTFSVGADHLLKIWKFKSKEHLHTHRLPAAPASSLLHRDSGMLAIALDVFAIHILDVETRRIVRKFSGHRGQINDMTFSPDGRWLITASMDCTIRTWDLPSGSLVDCFLVEAAAVSVSLSPTGNFLASSHVDGLGVYLWSNNTLCSLVSLRPLPADYEPTVIMLPGNCPSKDTEEEEVLDSDSSEMAEYISPAQLDEHLVTLSLLPDSRWKNLLHLDIIKKKNKPKEPPKVPKAAPFFIPTIPGLVPQFSLPNTPSEAQSKVVNLGVLAQKSNFYIELENAIESNSYEEPVKLLRELGPSAIETELRALSPDMGGDVGVMKSFLKMISCILKSKKDFDLAQAYLALFLKLHLRLISDEPELMEEAERVSAQLEETWMCIQTLLNQSICLLSYIKSALL

Target ORF information:

RefSeq Version	NM_001308831.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WD repeat domain 36 (wdr36), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308831.1

ORF Insert Sequence:

ATGAGCAGCA TGTCTGCGAA CAAAAAAGGG AGCGTGATCT TCTCTGGATT CAGGGCGCTG 
GGGCTTTACT CTAATCATCT GGCGCACGTG GTTCGCTTCC ATAAGAAACA CAGAGAGTTC 
TATGTGGTCA CTGCAGTCGG ACAATGTTTC CATACATATA ACGTAAAGAA ACTAGGAATC 
GTTGCAGTCA GTAATGCCCT CCCAGAAGAC ATTTCATCCG TGACAGCAGA TTGTATGCTT 
GTCTATGCAG CATATGGAAA ACTTATCTCC GCTTTTGCTA GAAGCAGAGA GGTTGTTCAC 
ACATACACCG GTCACCAAGC TGATGTGCAT TTGCTGCTGC CTTTTGGAAA TCATTTGATT 
TCTGTTGATA AGGATAATGT GGTTATCATT TGGGATGTGG AGTCTGAGGA CACATATCTG 
CAGATTTCAT ATGACAAAGC ATCTTTTGAG GTCTCCGCTT TGATGCATCC GAGCACCTAC 
CTGAACAAAA TCCTGTTTGG AAGCAGTCAA GGGAGTCTTC AGTTATGGAA CATCAAATCA 
AACAAACTCC TGTTCACATT TTCTGGATGG TCAGCTGCTG TTACTGTTCT TCAACAGACC 
CCAGCTGTGG ATGTTGTAGG TGTGGGTTTA GCATCTGGCC AGATCATCAT TCACAATATT 
AAATTTGACG AAACACTTAT GAAGTTCCAA CAAGACTGGG GTCCAATCAC AGCTTTATCC 
TTCAGGACAG ATGGACATCC AATAATGGCA TCTGGAAGTC CTATTGGACA CATAGGACTG 
TGGGATTTAG AGGAGAAGAA ACTAGTCAGT CAAATGCGAG ATGCTCATAC AACCGCAATC 
GCTGGCCTCA CGTTTCTTCA AAATGAACCT CTTTTGATCA CCAACGGAGC GGATAATGCA 
ATACGGGTGT GGATTTTTGA CGTTGCTGGT GGAGATGGTC GTCTGCTCAG ACAGAGGATG 
GGTCACAGCG CCCCACCAAC AAAAATTCGC CATTATGATC AAAGTGGCTT GAACATTCTT 
AGCGCAGGTC AAGATGGCAC TTTGCAGTCC TTTTCGACTG TTCATGAAAG GTTCAACAAG 
AGTTTGGGGC ATGGAACCCT CAACAAAGCA AAAGCTAAAA AGAAAGGCGT GAAGTATGAC 
ACAGCGAAAC TCCCATCCAT CACAACATTT GCTTCAGAAA CAGCACGTCA GAGTGACTGG 
GATGGCATTA TTGCATGCCA TCGTGGCTTT CTTATGACAA CATCATGGAA CTACCAGAAG 
GGTTCAATGG GTGCGCACAA ACTGGAGCCT GAACGATTCA ACAGGAACCG TAGCCTCAAT 
GTCCATGCAA CTGCTGTGGA TATCAGCTCT TGTGGGAACT TTGCTGTGAT TGCGCTGTCT 
TCGGGACACA TCGATGTGTA CAACATGCAG TCTGGTCTTC ATCGTGGGCA TTATGGAGAG 
GACAAGGCAC ATGAAGGGCC TGTGCGAGGA GTGGCTGTGG ATGCTTTAAA CCAGTTGACT 
TTTAGTGTGG GAGCAGACCA TCTTCTGAAA ATCTGGAAGT TCAAATCAAA AGAGCACTTA 
CATACTCACA GGTTACCTGC AGCCCCGGCA TCATCTCTGC TTCACAGGGA CAGTGGGATG 
CTCGCCATTG CTCTAGATGT TTTTGCCATT CACATTTTGG ACGTGGAAAC TAGGAGGATT 
GTCCGCAAAT TCTCTGGACA TCGTGGCCAG ATCAATGACA TGACATTTAG TCCAGATGGA 
CGTTGGCTGA TTACAGCATC TATGGACTGC ACCATCAGAA CATGGGATCT TCCATCTGGC 
AGTTTGGTCG ACTGTTTCCT GGTAGAAGCA GCAGCCGTGA GTGTCAGTTT GTCCCCTACC 
GGGAACTTCC TGGCCTCGTC GCACGTTGAC GGCCTCGGTG TTTACTTATG GTCCAATAAC 
ACGCTGTGCA GTTTGGTGTC ATTGCGCCCT CTACCTGCAG ACTATGAGCC GACTGTGATC 
ATGCTGCCTG GCAATTGCCC AAGCAAAGAT ACAGAAGAGG AGGAGGTCTT AGACTCTGAC 
AGTTCTGAGA TGGCAGAGTA CATTTCTCCA GCACAGTTAG ATGAACATCT GGTCACTCTG 
TCACTGCTTC CTGACTCACG CTGGAAGAAC CTGCTGCATC TCGACATCAT CAAGAAAAAG 
AACAAACCCA AAGAGCCTCC AAAAGTGCCC AAAGCAGCAC CCTTCTTTAT TCCCACCATT 
CCAGGGCTGG TGCCCCAGTT TTCTCTTCCC AACACACCTT CAGAAGCCCA GTCTAAAGTT 
GTCAACTTAG GAGTATTGGC ACAGAAGTCC AACTTCTACA TTGAGCTTGA GAATGCTATA 
GAGAGCAACT CCTATGAAGA GCCGGTAAAG CTGTTGAGAG AGTTGGGGCC CTCTGCCATT 
GAAACAGAAC TTCGGGCCCT GTCTCCAGAT ATGGGTGGAG ATGTTGGAGT CATGAAGAGC 
TTCCTCAAGA TGATCAGCTG CATCCTTAAG TCCAAAAAAG ATTTTGACCT GGCCCAAGCC 
TATCTGGCAT TATTCCTAAA GCTGCATCTT CGGTTGATCT CGGATGAGCC TGAACTGATG 
GAGGAGGCAG AGAGAGTGTC TGCGCAGCTG GAGGAGACGT GGATGTGCAT ACAGACGCTC 
TTAAACCAGA GCATTTGTCT GTTGTCTTAT ATCAAGAGTG CGCTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The primary open-angle glaucoma gene WDR36 functions in ribosomal RNA processing and interacts with the p53 stress-response pathway.
Human molecular genetics17(16)2474-85(2008 Aug)
Skarie JM,Link BA

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

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wdr36 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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