rock2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rock2a
Entrez Gene ID	317747
Full Name	rho-associated, coiled-coil containing protein kinase 2a
Synonyms	rock2,rok2
General protein information	Preferred Names rho-associated, coiled-coil containing protein kinase 2a Names rho-associated protein kinase 2 rho-associated, coiled-coil containing protein kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174863.2	NP_777288.1	rho-associated protein kinase 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
Zhang J, Jiang Z, Liu X, Meng A
Development (Cambridge, England)143(14)2603-15(2016 07)

Identification of apoptosis and macrophage migration events in paraquat-induced oxidative stress using a zebrafish model.
Wang Q, Liu S, Hu D, Wang Z, Wang L, Wu T, Wu Z, Mohan C, Peng A
Life sciences157116-24(2016 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina.
Lahne M, Li J, Marton RM, Hyde DR
The Journal of neuroscience : the official journal of the Society for Neuroscience35(47)15612-34(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Double knockdown of rock2a disrupted interkinetic nuclear migration in the retina.
Title: Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina.

The findings of the present study indicate that miR-142-3p plays a critical role in hematopoiesis, cardiogenesis, and somitegenesis in the early stage of mesoderm formation via regulation of Rock2a.
Title: miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in zebrafish.

Data show that Rock2 acts as a negative regulator of the TGFbeta signaling pathway.
Title: Rock2 controls TGFbeta signaling and inhibits mesoderm induction in zebrafish embryos.

The following rock2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rock2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35086	NM_174863.2 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2a (rock2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rock2a ( NM_174863.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_777288.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2a (rock2a), mRNA.

Price & Availability↑

CloneID	ODa35086
Clone ID Related Accession (Same CDS sequence)	NM_174863.2
Accession Version	NM_174863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	rho-associated protein kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF295804.1. On Jun 3, 2003 this sequence version replaced NM_174863.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF295804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCTAG GAGCGGAGAG AAGGATGGAA AACCGGCTGA GACAACTGGA AGCCATGATC 
AAAGACCCGC GGTCTGCGAT CAACTTGGAA AGTTTGCTGG ACTCCATGAA TGCCCTGGTT 
TTAGATCTGG ACTTTCCGGC CTTACGTAAA AATAAAAACA TCGAGAATTT CTTGAACAGA 
TATGAGAAAG TCATGAACCA TATAAGGGAG CTCCAGATGA GGCCAGAGGA TTTTGACAGA 
GTGAAGGTGA TTGGTAGAGG AGCATTCGGG GAAGTCCAGC TGGTTAGGCA TAAGGCCTCT 
CAGAAAGTGT ACGCCATGAA GGTGCTCAGC AAGTTCGAAA TGATCAAACG CTCAGATTCT 
GCCTTCTTCT GGGAGGAACG GGACATCATG GCGTTTGCAG ACAGCCCATG GGTCGTACAG 
CTGTGCTGTG CGTTCCAGGA TGACCGCTCT CTCTACATGG TAATGGAGTA CATGCCAGGT 
GGAGATCTAG TCAATCTCAC CAGCACATAT GATGTCCCAG AGAAGTGGGC AAAGTTTTAC 
ACAGCAGAGG TGGTGTTGGC TTTAGACGCT ATTCACTCCA TGGGCTTCAT TCATCGAGAC 
GTCAAACCAG ACAACATGCT GCTGGACCGC TACGGACACC TCAAACTGGC AGACTTCGGG 
ACCTGCATGA AAATGGATGG GACAGGGATG GTTCATTGTG ACACCGCTGT AGGAACCCCA 
GACTACATTT CGCCGGAGGT TTTGAAGTCT CAGGGAGGAG ACGGATACTA TGGTCGCGAG 
TGTGACTGGT GGTCAGTGGG AGTGTTTATC TACGAGATGC TGGTTGGTGA TACACCGTTC 
TACGCTGATT CTCTTGTGGG GACATACAGC AAGATTATGG ACCATAAGAA CTCTCTCAAC 
TTCCCTGATG ATGTTGAGAT CTCTCAAGAA GGCAAAAACA TTATCTGTGC CTTCCTTACT 
GACAGGGAGG TTCGCCTGGG TCGAAGTGGT GTAGAAGAAA TTAAAAGACA TCCTTTCTTT 
AGAAATGACC AGTGGACCTT CAGCACTATT AGAGAGACTG CAGCTCCTGT GGTTCCAGAG 
CTGAGCAGTG ACATAGACAC CAGTAATTTT GATGAAATTG AGGAGGATAA AGGAGAAGTG 
GAGACCTTTC CCACACCTAA AGCCTTTGTT GGGAATCAGC TGCCCTTTGT GGGATTCACC 
TACTTCAAAG AGAACCAGTT GTTGAATGCT GTGAACAGTT CAGCAATGAA AAATGACCAC 
CCAGTATCTA ACAGAGAGGA CAATTTAGCC CTCCAGAAGA AGCTTCACTG TCTAGAAGAG 
CAGCTCAACA ATGAAATGCA GGCCAAAGAT GAGTTGGAGC AGAAATACAG AAGCAACAGT 
AACCGCCTGG AAAAGATCAC CAAAGAGCTC GATGAAGAGA TGAATAGCAG GAAAGGTCTG 
GAGTCGACCC TGAGACAGCT GGAGAGAGAG AAGGCTCTGC TGCAGCACAA GAGTGTGGAG 
AGTCACCGCA GGGCCGAGAG CGAGGCCGAC CGCAAACGCT GCCTGGAGAA TGAAGTCAAT 
AGTCTGAGGG ATCAGCTGGA TGAAATGAAG AAGAAGAACC AGAACTCGCA CATCTCCAAC 
GAGAAGAACA TCCACCTGCA GAAACAGCTG GATGAGGCTA ATGCTTTACT GCGGGCAGAA 
TCTGAGGTGG CCACACGGAT GCGTAAAACT CAGACGGAGA GCTCCAAACA GCTGCAGCAG 
CTGGAGGCGC ATGTAAGAGA ACTGCAGGAC AAATGCTGCA TGCTGGAGAA CAGTAAGCTC 
ACGCTGGAGA GGGAGAACAT CAGCCTGCAG GCCGCACTGG ACACAGAGAA ACGAGAGCAG 
ACACAGGGAT CCGAGACCAT CTCTGATCTA CAGGCACGAA TCACTGGTAT GGAGGATGAG 
GTGAGGCAAA TGAGGCAGGC CCTATCTAAG GCTGAGACGG AAAAAAGGCA ACTTCAGGAG 
AAGCTCACAG ATATGGAGAA GGAGAAGAGC AATAACCAAA TTGACATGAC CTATAAGCTG 
AAGATGTTGC AGCAGGGTTT GGAGCAGGAG GAGGCAGCAC ACAAGGCCAC TAAAGCTCGA 
CTCGCTGACA AAAACATGAT CAGCGAGTCC ATCGAAGGAG CCAAATCTGA AGCTGTAAAG 
GAGCTGGAGC AAAAGCTGCA GGAGGAACGT TCCTCTAAAC AGCGTGTGGA GAACAGGGTG 
TTAGAACTGG AGAAGAAGAA CTCCATGCTC GACTGTGACT ATAAACAATC GCTCCAAAAA 
CTAGAGGAAC TGCGGCGACA CAAGGAGAGA CTCACAGAAG AGGTGAAGAA TCTGAACCTG 
AAGATTGAAC AAGAGGTTCA GAAACGCACT TTGACTCAGA ATGACCTGAA GGTTCAGAAC 
CAGCAGCTCA GCACCCTGAG AACATCAGAG AAACAGCTCA AACAGGAGAT CAACCACATT 
CTAGAAATTA AACGCAGCCT GGAAAAACAA AACATGGAAC TGCGCAAGGA ACGTCAGGAT 
TCTGATGGGC AGATGAAGGA ACTGCAGGAT CAGTTGGAAG CAGAGCAGTA TTTCTCTACG 
CTGTATAAGA CACAAGTGCG AGAGCTGAAG GAGGAGTGTG AGGAGAAAAA TAAACTTTAT 
AAAGACGTGC AGCAGAATCT ACAGGAGCTG CAGGAGGAAA GGGATTCACT TGCGGCCCAG 
TTGGAGATCA CACTAACCAA AGCAGACTCT GAGCAGCTGG CACGCTCTAT TGCGGAGGAG 
CAGTATTCAG ACCTAGAGAA AGAGAAGATC ATGAAGGAGC TGGAGATCAA GGAGATGATG 
GCCAGACACC GACAGGAGCT CGCTGAGAAA GACACCACCA TCAGCTCCTT AGAGGAAGCC 
AATCGCACAT TGACCAGTGA TGTGGCAAAC CTGGCCAATG AGAAGGAAGA ATTCAACAAC 
AAGCTGAAGG AGGCAGAGGA CTACCTGCAG AATCTGAAGA ATGAAGAGCA ATCCATCACT 
CAGGTCAAGC TAGCGCTGGA GAAACAACTG CAGTCAGAGA GAACCCTCAA AACACAGGCG 
GTGAACAAGC TGGCAGAGAT CATGAACAGA AAGGAGGTCA GAGGTGGTGG CAGCCGCCGT 
GGAAATGATA CAGACGTGAG GAGGAAGGAG AAAGAAAACA GGAAGCTGCA GCTGGAGCTT 
CGTTCAGAAA GAGAGAAGCT CAACAGCACA ATCATCAAAT ACCAGAGAGA GATCAACGAC 
ATACAAGCGC AACTGTTGGA TGAGTCTCAG GTGCGCATTG AGCTTCAGAT GGCTCTGGAC 
AGTAAAGACA GTGATATTGA GCAGCTGCGG AGTCTGCTGA ACTCACTCAA CGTTCAGTCG 
CTGGACTCGG CCAGTATGAG CAGCGGTCCA GACATGGACA CAGATGAGTC TTTGCTTGAG 
ATCAGATTGG AGGGTTGGCT GTCTTTACCT GTCAGAAACA ACACCAAACG CTTTGGATGG 
GAAAGAAAGT ATGTTGTGGT GAGCAGCAAG AAGATCCTCT TTTACAACAG CGAACAGGAC 
AAAGAGCACT CCAACCCGTA CATGGTGTTG GACATAGATA AACTTTTCCA TGTGCGCTCT 
GTAACCCAGA CGGATGTTTA TCGCGCTGAC GCCAAAGAAA TCCCCAGAAT ATTCCAGATT 
TTGTATGCTA ACGAGGGCGA GAGCAAGAAA GAGCAGGAAC TGGAGCCTCT TCCAGGTGAC 
AAGTCAAGCT ACATTTGCCA TAAGGGTCAC GAGTTCATCC CCACCCTCTA TCACTTCCCC 
ACCAACTGTG AAGCCTGTAC CAAACCCCTG TGGAACATGT TTAAGCCACC GCCTGCCCTC 
GAATGCAGAC GATGCCACAT CAAGTGCCAC AAAGACCACA TGGATAAGAA GGAGGAAGTC 
ATCGCGCCCT GCAGAGTCGA CATGTCTACA GCTAAGAATC TGCTGCTGTT AGCTTCGTCT 
CAGGAGGACC AGCAGAAGTG GGTTAGCCGT CTAATGAAGA AAATCCCCAA AAAACCTCCA 
GCCCCAGACG CCCCTCACCG CTCCTCTCCT AGAGTCCCTG TCAAAGTACA AAGCAGTCAA 
TCCATGAGGA GACCCAGTCG GCAGATACCC GCCGGCAAAC CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777288.1
CDS	98..4225
Translation	MSLGAERRMENRLRQLEAMIKDPRSAINLESLLDSMNALVLDLDFPALRKNKNIENFLNRYEKVMNHIRELQMRPEDFDRVKVIGRGAFGEVQLVRHKASQKVYAMKVLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRSLYMVMEYMPGGDLVNLTSTYDVPEKWAKFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDRYGHLKLADFGTCMKMDGTGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIYEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISQEGKNIICAFLTDREVRLGRSGVEEIKRHPFFRNDQWTFSTIRETAAPVVPELSSDIDTSNFDEIEEDKGEVETFPTPKAFVGNQLPFVGFTYFKENQLLNAVNSSAMKNDHPVSNREDNLALQKKLHCLEEQLNNEMQAKDELEQKYRSNSNRLEKITKELDEEMNSRKGLESTLRQLEREKALLQHKSVESHRRAESEADRKRCLENEVNSLRDQLDEMKKKNQNSHISNEKNIHLQKQLDEANALLRAESEVATRMRKTQTESSKQLQQLEAHVRELQDKCCMLENSKLTLERENISLQAALDTEKREQTQGSETISDLQARITGMEDEVRQMRQALSKAETEKRQLQEKLTDMEKEKSNNQIDMTYKLKMLQQGLEQEEAAHKATKARLADKNMISESIEGAKSEAVKELEQKLQEERSSKQRVENRVLELEKKNSMLDCDYKQSLQKLEELRRHKERLTEEVKNLNLKIEQEVQKRTLTQNDLKVQNQQLSTLRTSEKQLKQEINHILEIKRSLEKQNMELRKERQDSDGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKNKLYKDVQQNLQELQEERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHRQELAEKDTTISSLEEANRTLTSDVANLANEKEEFNNKLKEAEDYLQNLKNEEQSITQVKLALEKQLQSERTLKTQAVNKLAEIMNRKEVRGGGSRRGNDTDVRRKEKENRKLQLELRSEREKLNSTIIKYQREINDIQAQLLDESQVRIELQMALDSKDSDIEQLRSLLNSLNVQSLDSASMSSGPDMDTDESLLEIRLEGWLSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILYANEGESKKEQELEPLPGDKSSYICHKGHEFIPTLYHFPTNCEACTKPLWNMFKPPPALECRRCHIKCHKDHMDKKEEVIAPCRVDMSTAKNLLLLASSQEDQQKWVSRLMKKIPKKPPAPDAPHRSSPRVPVKVQSSQSMRRPSRQIPAGKPS

Target ORF information:

RefSeq Version	NM_174863.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2a (rock2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174863.2

ORF Insert Sequence:

ATGTCGCTAG GAGCGGAGAG AAGGATGGAA AACCGGCTGA GACAACTGGA AGCCATGATC 
AAAGACCCGC GGTCTGCGAT CAACTTGGAA AGTTTGCTGG ACTCCATGAA TGCCCTGGTT 
TTAGATCTGG ACTTTCCGGC CTTACGTAAA AATAAAAACA TCGAGAATTT CTTGAACAGA 
TATGAGAAAG TCATGAACCA TATAAGGGAG CTCCAGATGA GGCCAGAGGA TTTTGACAGA 
GTGAAGGTGA TTGGTAGAGG AGCATTCGGG GAAGTCCAGC TGGTTAGGCA TAAGGCCTCT 
CAGAAAGTGT ACGCCATGAA GGTGCTCAGC AAGTTCGAAA TGATCAAACG CTCAGATTCT 
GCCTTCTTCT GGGAGGAACG GGACATCATG GCGTTTGCAG ACAGCCCATG GGTCGTACAG 
CTGTGCTGTG CGTTCCAGGA TGACCGCTCT CTCTACATGG TAATGGAGTA CATGCCAGGT 
GGAGATCTAG TCAATCTCAC CAGCACATAT GATGTCCCAG AGAAGTGGGC AAAGTTTTAC 
ACAGCAGAGG TGGTGTTGGC TTTAGACGCT ATTCACTCCA TGGGCTTCAT TCATCGAGAC 
GTCAAACCAG ACAACATGCT GCTGGACCGC TACGGACACC TCAAACTGGC AGACTTCGGG 
ACCTGCATGA AAATGGATGG GACAGGGATG GTTCATTGTG ACACCGCTGT AGGAACCCCA 
GACTACATTT CGCCGGAGGT TTTGAAGTCT CAGGGAGGAG ACGGATACTA TGGTCGCGAG 
TGTGACTGGT GGTCAGTGGG AGTGTTTATC TACGAGATGC TGGTTGGTGA TACACCGTTC 
TACGCTGATT CTCTTGTGGG GACATACAGC AAGATTATGG ACCATAAGAA CTCTCTCAAC 
TTCCCTGATG ATGTTGAGAT CTCTCAAGAA GGCAAAAACA TTATCTGTGC CTTCCTTACT 
GACAGGGAGG TTCGCCTGGG TCGAAGTGGT GTAGAAGAAA TTAAAAGACA TCCTTTCTTT 
AGAAATGACC AGTGGACCTT CAGCACTATT AGAGAGACTG CAGCTCCTGT GGTTCCAGAG 
CTGAGCAGTG ACATAGACAC CAGTAATTTT GATGAAATTG AGGAGGATAA AGGAGAAGTG 
GAGACCTTTC CCACACCTAA AGCCTTTGTT GGGAATCAGC TGCCCTTTGT GGGATTCACC 
TACTTCAAAG AGAACCAGTT GTTGAATGCT GTGAACAGTT CAGCAATGAA AAATGACCAC 
CCAGTATCTA ACAGAGAGGA CAATTTAGCC CTCCAGAAGA AGCTTCACTG TCTAGAAGAG 
CAGCTCAACA ATGAAATGCA GGCCAAAGAT GAGTTGGAGC AGAAATACAG AAGCAACAGT 
AACCGCCTGG AAAAGATCAC CAAAGAGCTC GATGAAGAGA TGAATAGCAG GAAAGGTCTG 
GAGTCGACCC TGAGACAGCT GGAGAGAGAG AAGGCTCTGC TGCAGCACAA GAGTGTGGAG 
AGTCACCGCA GGGCCGAGAG CGAGGCCGAC CGCAAACGCT GCCTGGAGAA TGAAGTCAAT 
AGTCTGAGGG ATCAGCTGGA TGAAATGAAG AAGAAGAACC AGAACTCGCA CATCTCCAAC 
GAGAAGAACA TCCACCTGCA GAAACAGCTG GATGAGGCTA ATGCTTTACT GCGGGCAGAA 
TCTGAGGTGG CCACACGGAT GCGTAAAACT CAGACGGAGA GCTCCAAACA GCTGCAGCAG 
CTGGAGGCGC ATGTAAGAGA ACTGCAGGAC AAATGCTGCA TGCTGGAGAA CAGTAAGCTC 
ACGCTGGAGA GGGAGAACAT CAGCCTGCAG GCCGCACTGG ACACAGAGAA ACGAGAGCAG 
ACACAGGGAT CCGAGACCAT CTCTGATCTA CAGGCACGAA TCACTGGTAT GGAGGATGAG 
GTGAGGCAAA TGAGGCAGGC CCTATCTAAG GCTGAGACGG AAAAAAGGCA ACTTCAGGAG 
AAGCTCACAG ATATGGAGAA GGAGAAGAGC AATAACCAAA TTGACATGAC CTATAAGCTG 
AAGATGTTGC AGCAGGGTTT GGAGCAGGAG GAGGCAGCAC ACAAGGCCAC TAAAGCTCGA 
CTCGCTGACA AAAACATGAT CAGCGAGTCC ATCGAAGGAG CCAAATCTGA AGCTGTAAAG 
GAGCTGGAGC AAAAGCTGCA GGAGGAACGT TCCTCTAAAC AGCGTGTGGA GAACAGGGTG 
TTAGAACTGG AGAAGAAGAA CTCCATGCTC GACTGTGACT ATAAACAATC GCTCCAAAAA 
CTAGAGGAAC TGCGGCGACA CAAGGAGAGA CTCACAGAAG AGGTGAAGAA TCTGAACCTG 
AAGATTGAAC AAGAGGTTCA GAAACGCACT TTGACTCAGA ATGACCTGAA GGTTCAGAAC 
CAGCAGCTCA GCACCCTGAG AACATCAGAG AAACAGCTCA AACAGGAGAT CAACCACATT 
CTAGAAATTA AACGCAGCCT GGAAAAACAA AACATGGAAC TGCGCAAGGA ACGTCAGGAT 
TCTGATGGGC AGATGAAGGA ACTGCAGGAT CAGTTGGAAG CAGAGCAGTA TTTCTCTACG 
CTGTATAAGA CACAAGTGCG AGAGCTGAAG GAGGAGTGTG AGGAGAAAAA TAAACTTTAT 
AAAGACGTGC AGCAGAATCT ACAGGAGCTG CAGGAGGAAA GGGATTCACT TGCGGCCCAG 
TTGGAGATCA CACTAACCAA AGCAGACTCT GAGCAGCTGG CACGCTCTAT TGCGGAGGAG 
CAGTATTCAG ACCTAGAGAA AGAGAAGATC ATGAAGGAGC TGGAGATCAA GGAGATGATG 
GCCAGACACC GACAGGAGCT CGCTGAGAAA GACACCACCA TCAGCTCCTT AGAGGAAGCC 
AATCGCACAT TGACCAGTGA TGTGGCAAAC CTGGCCAATG AGAAGGAAGA ATTCAACAAC 
AAGCTGAAGG AGGCAGAGGA CTACCTGCAG AATCTGAAGA ATGAAGAGCA ATCCATCACT 
CAGGTCAAGC TAGCGCTGGA GAAACAACTG CAGTCAGAGA GAACCCTCAA AACACAGGCG 
GTGAACAAGC TGGCAGAGAT CATGAACAGA AAGGAGGTCA GAGGTGGTGG CAGCCGCCGT 
GGAAATGATA CAGACGTGAG GAGGAAGGAG AAAGAAAACA GGAAGCTGCA GCTGGAGCTT 
CGTTCAGAAA GAGAGAAGCT CAACAGCACA ATCATCAAAT ACCAGAGAGA GATCAACGAC 
ATACAAGCGC AACTGTTGGA TGAGTCTCAG GTGCGCATTG AGCTTCAGAT GGCTCTGGAC 
AGTAAAGACA GTGATATTGA GCAGCTGCGG AGTCTGCTGA ACTCACTCAA CGTTCAGTCG 
CTGGACTCGG CCAGTATGAG CAGCGGTCCA GACATGGACA CAGATGAGTC TTTGCTTGAG 
ATCAGATTGG AGGGTTGGCT GTCTTTACCT GTCAGAAACA ACACCAAACG CTTTGGATGG 
GAAAGAAAGT ATGTTGTGGT GAGCAGCAAG AAGATCCTCT TTTACAACAG CGAACAGGAC 
AAAGAGCACT CCAACCCGTA CATGGTGTTG GACATAGATA AACTTTTCCA TGTGCGCTCT 
GTAACCCAGA CGGATGTTTA TCGCGCTGAC GCCAAAGAAA TCCCCAGAAT ATTCCAGATT 
TTGTATGCTA ACGAGGGCGA GAGCAAGAAA GAGCAGGAAC TGGAGCCTCT TCCAGGTGAC 
AAGTCAAGCT ACATTTGCCA TAAGGGTCAC GAGTTCATCC CCACCCTCTA TCACTTCCCC 
ACCAACTGTG AAGCCTGTAC CAAACCCCTG TGGAACATGT TTAAGCCACC GCCTGCCCTC 
GAATGCAGAC GATGCCACAT CAAGTGCCAC AAAGACCACA TGGATAAGAA GGAGGAAGTC 
ATCGCGCCCT GCAGAGTCGA CATGTCTACA GCTAAGAATC TGCTGCTGTT AGCTTCGTCT 
CAGGAGGACC AGCAGAAGTG GGTTAGCCGT CTAATGAAGA AAATCCCCAA AAAACCTCCA 
GCCCCAGACG CCCCTCACCG CTCCTCTCCT AGAGTCCCTG TCAAAGTACA AAGCAGTCAA 
TCCATGAGGA GACCCAGTCG GCAGATACCC GCCGGCAAAC CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
Development (Cambridge, England)143(14)2603-15(2016 07)
Zhang J,Jiang Z,Liu X,Meng A

Identification of apoptosis and macrophage migration events in paraquat-induced oxidative stress using a zebrafish model.
Life sciences157116-24(2016 Jul)
Wang Q,Liu S,Hu D,Wang Z,Wang L,Wu T,Wu Z,Mohan C,Peng A

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(47)15612-34(2015 Nov)
Lahne M,Li J,Marton RM,Hyde DR

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rock2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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