ttc27 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttc27
Entrez Gene ID	317640
Full Name	tetratricopeptide repeat domain 27
Synonyms	chunp6906,flj20272l,hi1257,im:7158386,wu:fj40h09
General protein information	Preferred Names tetratricopeptide repeat domain 27 Names tetratricopeptide repeat protein 27
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467108.1	XP_021322783.1	tetratricopeptide repeat protein 27 isoform X1
NM_173285.2	NP_775392.2	tetratricopeptide repeat protein 27

Pathways from BioSystems

Homologies

Homo sapiens (human)	TTC27	NP_060205.3
Canis lupus familiaris (dog)	TTC27	XP_532927.3
Bos taurus (cattle)	TTC27	NP_001069330.1
Rattus norvegicus (Norway rat)	Ttc27	NP_001100176.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006358	XP_316379.4
Eremothecium gossypii	AGOS_ADL177C	NP_983919.2
Pan troglodytes (chimpanzee)	TTC27	XP_001164860.2
Gallus gallus (chicken)	TTC27	NP_001012585.1
Danio rerio (zebrafish)	ttc27	NP_775392.2
Kluyveromyces lactis	KLLA0E07239g	XP_454275.1
	TTC27	XP_001106562.1
Caenorhabditis elegans (roundworm)	T20B12.1	NP_498636.2
Saccharomyces cerevisiae (baker's yeast)	EMW1	NP_014086.1
Magnaporthe oryzae (rice blast fungus)	MGG_09846	XP_003720393.1
Mus musculus (house mouse)	Ttc27	NP_690030.3
Drosophila melanogaster (fruit fly)	CG5290	NP_649026.1
Schizosaccharomyces pombe (fission yeast)	SPAC19B12.01	NP_594764.2
Neurospora crassa	NCU09658	XP_958724.1
Xenopus tropicalis (tropical clawed frog)	ttc27	XP_002934504.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Trinh le A, Hochgreb T, Graham M, Wu D, Ruf-Zamojski F, Jayasena CS, Saxena A, Hawk R, Gonzalez-Serricchio A, Dixson A, Chow E, Gonzales C, Leung HY, Solomon I, Bronner-Fraser M, Megason SG, Fraser SE
Genes & development25(21)2306-20(2011 Nov)

Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
Golling G, Amsterdam A, Sun Z, Antonelli M, Maldonado E, Chen W, Burgess S, Haldi M, Artzt K, Farrington S, Lin SY, Nissen RM, Hopkins N
Nature genetics31(2)135-40(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ttc27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttc27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa149513	XM_021467108.1 Latest version!	Danio rerio tetratricopeptide repeat domain 27 (ttc27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa34897	NM_173285.2 Latest version!	Danio rerio tetratricopeptide repeat domain 27 (ttc27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttc27 ( XM_021467108.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322783.1 Danio rerio tetratricopeptide repeat domain 27 (ttc27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149513
Clone ID Related Accession (Same CDS sequence)	XM_021467108.1
Accession Version	XM_021467108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tetratricopeptide repeat protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGC TCGAGGCGGC GGTTCTGGGC GGATTGAGTC TGGACTGGAA ACAGAGCAGC 
TTCAGCACCG CAGATGCAGG TTCATTGCTG CAGTTGGTAT ATGACGGAGA TTTTGAGGCC 
GTCCTCTTCA GCTCTGCGGT GCAGGGGCTT CTGGGAGTTT GTCCTGAAGA GGGTGACAGC 
ATTGAGGCCT ATCTGGAGAG ACAGGTGCTG TCTTACCTGA GTGATGCCAC CGAAGAGCAG 
AGGAGCCACA GGGAAGTTGC TCTGTTGGCG GTGGCAGTGG GGTGTCTGAA CCTTTTCGCC 
CAGAGTAACT GGACCGGCCC ACCAGTTGAG CTCCATGTCT CTGATGTTCT TCCTGAAGCC 
CTGCTCCAGA ACTTTTCTCA GCCTGCGGCC CTGAGTACAG CTCTGTTGTC CAGTCTTCAG 
CTGGATGGAG AGTCTGTGTA CAGTCTGGTC TCCAACCCTC TCCTGCTGCT GCTGACCAGA 
GTCATCTTCG TCAACTGCGC CGCCAAACTG GAGAGCCTTC AGCTGCTGCC CTGGTGGACG 
CTTCGATATG TCGCTCTCCA TCAGCAGATA CTGGAGGAAA GATCCCCACA GCTCCTCAAC 
CTGATGCTCA GCTGCATCGA GAAAGTGTGT AAATGTGACG AGCTGTTCAG CAGTAATGCA 
CACAGAAACC TGGCCATTCA GTTCCACCTG GAGTGCAGTT ATATCTGCCT GACCTACTAT 
GAGTATCACA AGGCCAAAGA ACATCTGCAG ACGGCTCGTG ATCTGTCAGG ACTCGATGTT 
AACATGACAG GTGCTCTAGG GAAGCGAACT CGTTTTCAGG AGAACTTTTT GGCTCAGCTC 
ATCTTAGATG TAAAGCGAAA GGACAACTCG CCCTTTTACA ACTCCGAGAG TCCCTCCAGT 
ACACCAACAC CTAAAGAGCT CCTACCAAAG GACCATCAGC TGAGCGACGA TACAGTCCTA 
AACCAGATTA ATCTTGCAGA ACCATCTGAG CATGAACTTC CAGACCTAAG CGCTGAGGAG 
CAAGCCTTCA TACTGGCCAC ATGCACAGAC TTCCAGAAGA ATAATCCAGT TCACAAGCTG 
AATGATGAGG AGCTCCTGGC CTTCACCACG TGTTTGCTGT CTCAGCCTAA GTTCTGGGCT 
GTGGAAGTGA CATCTCTGTG TCTGAGGACT AAGCTGGAGA GAGGAAGCTC ACGGAGAGTT 
GAACGTGCCA TGATGCAAAC ACAGACTCTG GTGGATTTCT TCAGTGAGAA GGACTGTCCC 
GTCACGGAGA GGCTGAAGAT GTTCTACAGC TGCCGGGCTC CTCCTCTCTG GGATCTACAG 
TCTTTTTTTC ACTTTCATCA TTTACTACTT TCATCTGTTC GTCTTTTCTT TTGTCCTTTT 
ATCCTATTTT TGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322783.1
CDS	279..1676
Translation	MLQLEAAVLGGLSLDWKQSSFSTADAGSLLQLVYDGDFEAVLFSSAVQGLLGVCPEEGDSIEAYLERQVLSYLSDATEEQRSHREVALLAVAVGCLNLFAQSNWTGPPVELHVSDVLPEALLQNFSQPAALSTALLSSLQLDGESVYSLVSNPLLLLLTRVIFVNCAAKLESLQLLPWWTLRYVALHQQILEERSPQLLNLMLSCIEKVCKCDELFSSNAHRNLAIQFHLECSYICLTYYEYHKAKEHLQTARDLSGLDVNMTGALGKRTRFQENFLAQLILDVKRKDNSPFYNSESPSSTPTPKELLPKDHQLSDDTVLNQINLAEPSEHELPDLSAEEQAFILATCTDFQKNNPVHKLNDEELLAFTTCLLSQPKFWAVEVTSLCLRTKLERGSSRRVERAMMQTQTLVDFFSEKDCPVTERLKMFYSCRAPPLWDLQSFFHFHHLLLSSVRLFFCPFILFLM

Target ORF information:

RefSeq Version	XM_021467108.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 27 (ttc27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467108.1

ORF Insert Sequence:

ATGCTGCAGC TCGAGGCGGC GGTTCTGGGC GGATTGAGTC TGGACTGGAA ACAGAGCAGC 
TTCAGCACCG CAGATGCAGG TTCATTGCTG CAGTTGGTAT ATGACGGAGA TTTTGAGGCC 
GTCCTCTTCA GCTCTGCGGT GCAGGGGCTT CTGGGAGTTT GTCCTGAAGA GGGTGACAGC 
ATTGAGGCCT ATCTGGAGAG ACAGGTGCTG TCTTACCTGA GTGATGCCAC CGAAGAGCAG 
AGGAGCCACA GGGAAGTTGC TCTGTTGGCG GTGGCAGTGG GGTGTCTGAA CCTTTTCGCC 
CAGAGTAACT GGACCGGCCC ACCAGTTGAG CTCCATGTCT CTGATGTTCT TCCTGAAGCC 
CTGCTCCAGA ACTTTTCTCA GCCTGCGGCC CTGAGTACAG CTCTGTTGTC CAGTCTTCAG 
CTGGATGGAG AGTCTGTGTA CAGTCTGGTC TCCAACCCTC TCCTGCTGCT GCTGACCAGA 
GTCATCTTCG TCAACTGCGC CGCCAAACTG GAGAGCCTTC AGCTGCTGCC CTGGTGGACG 
CTTCGATATG TCGCTCTCCA TCAGCAGATA CTGGAGGAAA GATCCCCACA GCTCCTCAAC 
CTGATGCTCA GCTGCATCGA GAAAGTGTGT AAATGTGACG AGCTGTTCAG CAGTAATGCA 
CACAGAAACC TGGCCATTCA GTTCCACCTG GAGTGCAGTT ATATCTGCCT GACCTACTAT 
GAGTATCACA AGGCCAAAGA ACATCTGCAG ACGGCTCGTG ATCTGTCAGG ACTCGATGTT 
AACATGACAG GTGCTCTAGG GAAGCGAACT CGTTTTCAGG AGAACTTTTT GGCTCAGCTC 
ATCTTAGATG TAAAGCGAAA GGACAACTCG CCCTTTTACA ACTCCGAGAG TCCCTCCAGT 
ACACCAACAC CTAAAGAGCT CCTACCAAAG GACCATCAGC TGAGCGACGA TACAGTCCTA 
AACCAGATTA ATCTTGCAGA ACCATCTGAG CATGAACTTC CAGACCTAAG CGCTGAGGAG 
CAAGCCTTCA TACTGGCCAC ATGCACAGAC TTCCAGAAGA ATAATCCAGT TCACAAGCTG 
AATGATGAGG AGCTCCTGGC CTTCACCACG TGTTTGCTGT CTCAGCCTAA GTTCTGGGCT 
GTGGAAGTGA CATCTCTGTG TCTGAGGACT AAGCTGGAGA GAGGAAGCTC ACGGAGAGTT 
GAACGTGCCA TGATGCAAAC ACAGACTCTG GTGGATTTCT TCAGTGAGAA GGACTGTCCC 
GTCACGGAGA GGCTGAAGAT GTTCTACAGC TGCCGGGCTC CTCCTCTCTG GGATCTACAG 
TCTTTTTTTC ACTTTCATCA TTTACTACTT TCATCTGTTC GTCTTTTCTT TTGTCCTTTT 
ATCCTATTTT TGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttc27 ( NM_173285.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775392.2 Danio rerio tetratricopeptide repeat domain 27 (ttc27), mRNA.

Price & Availability↑

CloneID	ODa34897
Clone ID Related Accession (Same CDS sequence)	NM_173285.2
Accession Version	NM_173285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tetratricopeptide repeat protein 27
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548077.12, BX511309.12 and CT027669.8. On Jul 17, 2010 this sequence version replaced NM_173285.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01001521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06350654, SAMN06350656 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCAGC TCGAGGCGGC GGTTCTGGGC GGATTGAGTC TGGACTGGAA ACAGAGCAGC 
TTCAGCACCG CAGATGCAGG TTCATTGCTG CAGTTGGTAT ATGACGGAGA TTTTGAGGCC 
GTCCTCTTCA GCTCTGCGGT GCAGGGGCTT CTGGGAGTTT GTCCTGAAGA GGGTGACAGC 
ATTGAGGCCT ATCTGGAGAG ACAGGTGCTG TCTTACCTGA GTGATGCCAC CGAAGAGCAG 
AGGAGCCACA GGGAAGTTGC TCTGTTGGCG GTGGCAGTGG GGTGTCTGAA CCTTTTCGCC 
CAGAGTAACT GGACCGGCCC ACCAGTTGAG CTCCATGTCT CTGATGTTCT TCCTGAAGCC 
CTGCTCCAGA ACTTTTCTCA GCCTGCGGCC CTGAGTACAG CTCTGTTGTC CAGTCTTCAG 
CTGGATGGAG AGTCTGTGTA CAGTCTGGTC TCCAACCCTC TCCTGCTGCT GCTGACCAGA 
GTCATCTTCG TCAACTGCGC CGCCAAACTG GAGAGCCTTC AGCTGCTGCC CTGGTGGACG 
CTTCGATATG TCGCTCTCCA TCAGCAGATA CTGGAGGAAA GATCCCCACA GCTCCTCAAC 
CTGATGCTCA GCTGCATCGA GAAAGTGTGT AAATGTGACG AGCTGTTCAG CAGTAATGCA 
CACAGAAACC TGGCCATTCA GTTCCACCTG GAGTGCAGTT ATATCTGCCT GACCTACTAT 
GAGTATCACA AGGCCAAAGA ACATCTGCAG ACGGCTCGTG ATCTGTCAGG ACTCGATGTT 
AACATGACAG GTGCTCTAGG GAAGCGAACT CGTTTTCAGG AGAACTTTTT GGCTCAGCTC 
ATCTTAGATG TAAAGCGAAA GGACAACTCG CCCTTTTACA ACTCCGAGAG TCCCTCCAGT 
ACACCAACAC CTAAAGAGCT CCTACCAAAG GACCATCAGC TGAGCGACGA TACAGTCCTA 
AACCAGATTA ATCTTGCAGA ACCATCTGAG CATGAACTTC CAGACCTAAG CGCTGAGGAG 
CAAGCCTTCA TACTGGCCAC ATGCACAGAC TTCCAGAAGA ATAATCCAGT TCACAAGCTG 
AATGATGAGG AGCTCCTGGC CTTCACCACG TGTTTGCTGT CTCAGCCTAA GTTCTGGGCT 
GTGGAAGTGA CATCTCTGTG TCTGAGGACT AAGCTGGAGA GAGGAAGCTC ACGGAGAGTT 
GAACGTGCCA TGATGCAAAC ACAGACTCTG GTGGATTTCT TCAGTGAGAA GGACTGTCCC 
GTCACGGAGA GGCTGAAGAT GTTCTACAGC TGCCGGGCTC CTCCTCTCTG GGATCTACAG 
AGGCAGTTGG CGAGTCTGTT GACTGATTTG GGCCTGACTA GTTCTGCACT GCTGATCTAC 
GAGAGACTGG AGCTCTGGGA GGATGCAGTT GTGTGTCTGG AGAGGATGGG ACAGCATGGC 
AAGGCGGAGG AGATTTTGAG GCGTGAGTTG GAGAAAAAGG AGACGCCGTC GCTGTACTGT 
CTGCTGGGCG ATGTACTGAA GGATCCGCAG TATTATGACC GAGCATGGGA GCTTTCCAAA 
CACCGCAGCG CACGTGCCCA GCGCTCCAAA GCTCTGCATC ACCTGCGCCA CAGAGAGTTC 
CAGCAGTGTG TGGAGTGTTT CGAACGCTCT CTACAAATCA ACTCCATGCA GTTGGGTGTG 
TGGTTTTCCC TGGGCTGTGC ATATTTTGCA TTGGAAAGCT ACGAAGGAGC CGCCAGAGCT 
TTTCAAAGAT GCGTCGGACT GGAACCAGAT AATTCAGAAG CTTGGAACAA CCTTTCCACG 
GCCTACATCA AACTGAGAGT GAAGGAGAAG GCGTTCCGCA CCCTTCAGGA AGCGCTCAAG 
TGTAATTATG AGCGATGGCA GATCTGGGAG AATTTCATTG CGGTGTGCGT AGACCTGGGC 
GAGTTCAGTG AAGCTATCAG AGCCTACCAT CGGCTCATGG AGCTCAAGGA CAAATACAAG 
GACGTGGAGG TTCTGGAAAT CCTGGTGCAA GCAGTAGTGG ATAATCTAAC CGATCACCGG 
GGCGAACAGG CCTCCATTCT CAGACCCAAA CTCCAGGAGC TTTTGGGCAG AGTGTCGTCC 
CGCTGCAGCA CAGACGCACA GATATGGAAA CAGTACGCCC GTCTTTATGG CGATGGACAT 
AGCAACAATG CAGAAGACAA TGAAAAGGCG CTGCAGTTTC TGAGTAAAGC TCATCGATGT 
GAAACGCAGG CGGCTGGATG GGAGAAAGAC ACAGGCAGCT TCAAATCTGT GGTGAAAGGT 
GCCAGAGACA TGGCTAATGT GTCCATCAGC TGCAGCAGGT CGAAACGCAG TCCTCAAGAG 
GCTCTGCAGC TGCTGTCTTC AGCTCGACTC AGTCTGAAGA GTCTGACCAC CAAAGCCAGA 
CAACTGTACA CTGACGTGGC CACAGGTGAG CTCCACGATG ACCTCAGAGA TGATGTCATC 
GAACTGGAGC AGCTCATCAC AGAAATCCAG GATTTGAGCG CACAGCTGCG CAGCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775392.2
CDS	188..2707
Translation	MLQLEAAVLGGLSLDWKQSSFSTADAGSLLQLVYDGDFEAVLFSSAVQGLLGVCPEEGDSIEAYLERQVLSYLSDATEEQRSHREVALLAVAVGCLNLFAQSNWTGPPVELHVSDVLPEALLQNFSQPAALSTALLSSLQLDGESVYSLVSNPLLLLLTRVIFVNCAAKLESLQLLPWWTLRYVALHQQILEERSPQLLNLMLSCIEKVCKCDELFSSNAHRNLAIQFHLECSYICLTYYEYHKAKEHLQTARDLSGLDVNMTGALGKRTRFQENFLAQLILDVKRKDNSPFYNSESPSSTPTPKELLPKDHQLSDDTVLNQINLAEPSEHELPDLSAEEQAFILATCTDFQKNNPVHKLNDEELLAFTTCLLSQPKFWAVEVTSLCLRTKLERGSSRRVERAMMQTQTLVDFFSEKDCPVTERLKMFYSCRAPPLWDLQRQLASLLTDLGLTSSALLIYERLELWEDAVVCLERMGQHGKAEEILRRELEKKETPSLYCLLGDVLKDPQYYDRAWELSKHRSARAQRSKALHHLRHREFQQCVECFERSLQINSMQLGVWFSLGCAYFALESYEGAARAFQRCVGLEPDNSEAWNNLSTAYIKLRVKEKAFRTLQEALKCNYERWQIWENFIAVCVDLGEFSEAIRAYHRLMELKDKYKDVEVLEILVQAVVDNLTDHRGEQASILRPKLQELLGRVSSRCSTDAQIWKQYARLYGDGHSNNAEDNEKALQFLSKAHRCETQAAGWEKDTGSFKSVVKGARDMANVSISCSRSKRSPQEALQLLSSARLSLKSLTTKARQLYTDVATGELHDDLRDDVIELEQLITEIQDLSAQLRSQ

Target ORF information:

RefSeq Version	NM_173285.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 27 (ttc27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173285.2

ORF Insert Sequence:

ATGCTGCAGC TCGAGGCGGC GGTTCTGGGC GGATTGAGTC TGGACTGGAA ACAGAGCAGC 
TTCAGCACCG CAGATGCAGG TTCATTGCTG CAGTTGGTAT ATGACGGAGA TTTTGAGGCC 
GTCCTCTTCA GCTCTGCGGT GCAGGGGCTT CTGGGAGTTT GTCCTGAAGA GGGTGACAGC 
ATTGAGGCCT ATCTGGAGAG ACAGGTGCTG TCTTACCTGA GTGATGCCAC CGAAGAGCAG 
AGGAGCCACA GGGAAGTTGC TCTGTTGGCG GTGGCAGTGG GGTGTCTGAA CCTTTTCGCC 
CAGAGTAACT GGACCGGCCC ACCAGTTGAG CTCCATGTCT CTGATGTTCT TCCTGAAGCC 
CTGCTCCAGA ACTTTTCTCA GCCTGCGGCC CTGAGTACAG CTCTGTTGTC CAGTCTTCAG 
CTGGATGGAG AGTCTGTGTA CAGTCTGGTC TCCAACCCTC TCCTGCTGCT GCTGACCAGA 
GTCATCTTCG TCAACTGCGC CGCCAAACTG GAGAGCCTTC AGCTGCTGCC CTGGTGGACG 
CTTCGATATG TCGCTCTCCA TCAGCAGATA CTGGAGGAAA GATCCCCACA GCTCCTCAAC 
CTGATGCTCA GCTGCATCGA GAAAGTGTGT AAATGTGACG AGCTGTTCAG CAGTAATGCA 
CACAGAAACC TGGCCATTCA GTTCCACCTG GAGTGCAGTT ATATCTGCCT GACCTACTAT 
GAGTATCACA AGGCCAAAGA ACATCTGCAG ACGGCTCGTG ATCTGTCAGG ACTCGATGTT 
AACATGACAG GTGCTCTAGG GAAGCGAACT CGTTTTCAGG AGAACTTTTT GGCTCAGCTC 
ATCTTAGATG TAAAGCGAAA GGACAACTCG CCCTTTTACA ACTCCGAGAG TCCCTCCAGT 
ACACCAACAC CTAAAGAGCT CCTACCAAAG GACCATCAGC TGAGCGACGA TACAGTCCTA 
AACCAGATTA ATCTTGCAGA ACCATCTGAG CATGAACTTC CAGACCTAAG CGCTGAGGAG 
CAAGCCTTCA TACTGGCCAC ATGCACAGAC TTCCAGAAGA ATAATCCAGT TCACAAGCTG 
AATGATGAGG AGCTCCTGGC CTTCACCACG TGTTTGCTGT CTCAGCCTAA GTTCTGGGCT 
GTGGAAGTGA CATCTCTGTG TCTGAGGACT AAGCTGGAGA GAGGAAGCTC ACGGAGAGTT 
GAACGTGCCA TGATGCAAAC ACAGACTCTG GTGGATTTCT TCAGTGAGAA GGACTGTCCC 
GTCACGGAGA GGCTGAAGAT GTTCTACAGC TGCCGGGCTC CTCCTCTCTG GGATCTACAG 
AGGCAGTTGG CGAGTCTGTT GACTGATTTG GGCCTGACTA GTTCTGCACT GCTGATCTAC 
GAGAGACTGG AGCTCTGGGA GGATGCAGTT GTGTGTCTGG AGAGGATGGG ACAGCATGGC 
AAGGCGGAGG AGATTTTGAG GCGTGAGTTG GAGAAAAAGG AGACGCCGTC GCTGTACTGT 
CTGCTGGGCG ATGTACTGAA GGATCCGCAG TATTATGACC GAGCATGGGA GCTTTCCAAA 
CACCGCAGCG CACGTGCCCA GCGCTCCAAA GCTCTGCATC ACCTGCGCCA CAGAGAGTTC 
CAGCAGTGTG TGGAGTGTTT CGAACGCTCT CTACAAATCA ACTCCATGCA GTTGGGTGTG 
TGGTTTTCCC TGGGCTGTGC ATATTTTGCA TTGGAAAGCT ACGAAGGAGC CGCCAGAGCT 
TTTCAAAGAT GCGTCGGACT GGAACCAGAT AATTCAGAAG CTTGGAACAA CCTTTCCACG 
GCCTACATCA AACTGAGAGT GAAGGAGAAG GCGTTCCGCA CCCTTCAGGA AGCGCTCAAG 
TGTAATTATG AGCGATGGCA GATCTGGGAG AATTTCATTG CGGTGTGCGT AGACCTGGGC 
GAGTTCAGTG AAGCTATCAG AGCCTACCAT CGGCTCATGG AGCTCAAGGA CAAATACAAG 
GACGTGGAGG TTCTGGAAAT CCTGGTGCAA GCAGTAGTGG ATAATCTAAC CGATCACCGG 
GGCGAACAGG CCTCCATTCT CAGACCCAAA CTCCAGGAGC TTTTGGGCAG AGTGTCGTCC 
CGCTGCAGCA CAGACGCACA GATATGGAAA CAGTACGCCC GTCTTTATGG CGATGGACAT 
AGCAACAATG CAGAAGACAA TGAAAAGGCG CTGCAGTTTC TGAGTAAAGC TCATCGATGT 
GAAACGCAGG CGGCTGGATG GGAGAAAGAC ACAGGCAGCT TCAAATCTGT GGTGAAAGGT 
GCCAGAGACA TGGCTAATGT GTCCATCAGC TGCAGCAGGT CGAAACGCAG TCCTCAAGAG 
GCTCTGCAGC TGCTGTCTTC AGCTCGACTC AGTCTGAAGA GTCTGACCAC CAAAGCCAGA 
CAACTGTACA CTGACGTGGC CACAGGTGAG CTCCACGATG ACCTCAGAGA TGATGTCATC 
GAACTGGAGC AGCTCATCAC AGAAATCCAG GATTTGAGCG CACAGCTGCG CAGCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Genes & development25(21)2306-20(2011 Nov)
Trinh le A,Hochgreb T,Graham M,Wu D,Ruf-Zamojski F,Jayasena CS,Saxena A,Hawk R,Gonzalez-Serricchio A,Dixson A,Chow E,Gonzales C,Leung HY,Solomon I,Bronner-Fraser M,Megason SG,Fraser SE

Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
Nature genetics31(2)135-40(2002 Jun)
Golling G,Amsterdam A,Sun Z,Antonelli M,Maldonado E,Chen W,Burgess S,Haldi M,Artzt K,Farrington S,Lin SY,Nissen RM,Hopkins N

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ttc27 cDNA ORF clone, Danio rerio(zebrafish)

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