TEK cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TEK
Entrez Gene ID	30747
Full Name	TEK tyrosine kinase, endothelial
Synonyms	tie-2,tie2
General protein information	Preferred Names TEK tyrosine kinase, endothelial Names tyrosine-protein kinase receptor Tie-2 endothelium-specific receptor tyrosine kinase 2 tyrosine kinase with Ig and EGF homology domains-2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301113.3	XP_009299388.1	tyrosine-protein kinase receptor Tie-2 isoform X1
XM_017356039.1	XP_017211528.1	tyrosine-protein kinase receptor Tie-2 isoform X2
NM_131461.1	NP_571536.1	tyrosine-protein kinase receptor Tie-2 precursor
XM_021476003.1	XP_021331678.1	tyrosine-protein kinase receptor Tie-2 isoform X2
XM_009301114.2	XP_009299389.1	tyrosine-protein kinase receptor Tie-2 isoform X3
XM_021476005.1	XP_021331680.1	tyrosine-protein kinase receptor Tie-2 isoform X2
XM_009301113.2	XP_009299388.1	tyrosine-protein kinase receptor Tie-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TEK	NP_000450.2
Mus musculus (house mouse)	Tek	NP_038718.2
Xenopus tropicalis (tropical clawed frog)	tek	XP_002934031.2
	TEK	XP_001105270.1
Rattus norvegicus (Norway rat)	Tek	NP_001099207.1
Pan troglodytes (chimpanzee)	TEK	XP_520519.3
Canis lupus familiaris (dog)	TEK	XP_005626753.1
Bos taurus (cattle)	TEK	NP_776389.1
Gallus gallus (chicken)	TEK	XP_004949637.1
Danio rerio (zebrafish)	tek	NP_571536.1

Gene Ontology
Bibliography

Related articles in PubMed

Etv2-miR-130a-Jarid2 cascade regulates vascular patterning during embryogenesis.
Singh BN, Tahara N, Kawakami Y, Das S, Koyano-Nakagawa N, Gong W, Garry MG, Garry DJ
PloS one12(12)e0189010(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observations demonstrate that Tie2 is an important regulator of tip cell behaviors
Title: The zebrafish Tie2 signaling controls tip cell behaviors and acts synergistically with Vegf pathway in developmental angiogenesis.

Tie-1 and Tie-2 are not required for early heart development, yet they have redundant roles for the maintenance of endocardial-myocardial connection in later stages.
Title: Zebrafish Tie-2 shares a redundant role with Tie-1 in heart development and regulates vessel integrity.

The following TEK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TEK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142563	XM_021476003.1 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa142563	XM_021476005.1 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa01325	NM_131461.1 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa39325	XM_009301113.3 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa39326	XM_009301114.2 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa59054	XM_017356039.1 Latest version!	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
ODa39325	XM_009301113.2	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TEK ( XM_021476003.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331678.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa142563
Clone ID Related Accession (Same CDS sequence)	XM_021476003.1 , XM_021476005.1
Accession Version	XM_021476003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331678.1
CDS	855..3848
Translation	MLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEVVSLGGTPYCGMTCAELYEKLPLGFRLEKPLNCDDEVYELMQQCWREKPFERPSFSQILLSLGRMLEERKTYVNTTLYEKFTYAGIDCSAEEAG

Target ORF information:

RefSeq Version	XM_021476003.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476003.1

ORF Insert Sequence:

ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( XM_021476005.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331680.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa142563
Clone ID Related Accession (Same CDS sequence)	XM_021476003.1 , XM_021476005.1
Accession Version	XM_021476005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331680.1
CDS	852..3845
Translation	MLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEVVSLGGTPYCGMTCAELYEKLPLGFRLEKPLNCDDEVYELMQQCWREKPFERPSFSQILLSLGRMLEERKTYVNTTLYEKFTYAGIDCSAEEAG

Target ORF information:

RefSeq Version	XM_021476005.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476005.1

ORF Insert Sequence:

ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( NM_131461.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571536.1 Danio rerio TEK tyrosine kinase, endothelial (tek), mRNA.

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CloneID	ODa01325
Clone ID Related Accession (Same CDS sequence)	NM_131461.1
Accession Version	NM_131461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF053632.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF053632.1, BC163579.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCGTCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCAG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCC 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGACACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGCGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACATGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTGCCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TTGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCGTCACAG CGCACGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCAG CTTCCTCCTC CACCGGCCAA CATCTCCATC 
TGGAACATCA GCGATACCTC CGCCGTCCTC ACCTGGGCTG TGGCGGAGGG CGAGTCTGTG 
TCCAGAGCCG TGATCCGCTT CCAGCAGGTG GAGCAGGCGC AGTACAGACA GCAAGTGGAG 
CTCCCGGTGC AGACACAGCA GCTCCACATG CGCTTCCAGC TCCTGGGCCT GAGGCCCAAC 
ACCGGATACC AGCTGCAGCT GTGGACGGTC AATAACATGG GGGAGAGTGC GGAGAGTCCA 
CCGGTCAGCC TGATGACACT GCCACAGCAG GAGAGCTCAG CGCTGTTCGC GGCTCATGGA 
CACCTGCTCC TCTATGCTAT CCTGGGGTCC GCCGGCATGA CCTGCTGCAC CGTCCTGCTG 
GCCTTCTGCA TCGTCCTGCA GCTCAAGAGG AACACACTGC AGCGGCGCAT CCACAGCATA 
CTGAGGGAGG AGCCGGCGGT GCACTTCAGC TCAGCTCCGC CCCCTCATCG GCGCTCGGCG 
GTGGTCAGCC GCTCTCTGGT GTTCCCGGCG CTGCAGTGGA GTGATATCCA GTTCCAGGAC 
GTGCTCGGGG AGGGAAACTT CGGGCAGGTG CTGAAGGCGC GCATCAGGAA GGACGGGCTG 
AGGATGGATG CGGCCGTCAA GAGGATGAAA GATTACGCCT CTCAGGACGA CCACCGCGAC 
TTCGCCGGGG AGCTGGAGGT GCTGTGCAGA CTGGGCCCAC ACAAAAACAT CATACACCTG 
CTGGGAGCCT GCGAACACAG AGGTTACCTG TATCTGGCCA TCGAGTTCGC TCCTCATGGG 
AACCTGCTGG ACTTCCTGCG CAAGAGTCGT GTTCTGGAGA CCGACCCGGC CTTCGCCATC 
GCCCACCGCA CCGCCAGCAC ACTCTCCTCA CAGCAGCTGC TGGCCTTCAG TGCAGACGTG 
GCCCGCGGGA TGAGCTACCT GAGCCAGAAA CAGTTCATAC ACAGAGATCT AGCGGCTCGA 
AACGTCCTGG TGGGAGAGAA CTTTGTGGCG AAGATCGCAG ACTTCGGGCT GTCCAGAGGT 
CAGGAGGTGT ACGTCAAGAA GACCATGGGT CGTCTGCCAG TGCGATGGAT GGCAATAGAG 
TCGCTTAACT ACAGTGTGTA CACCACCAAC AGTGACGTGT GGTCGTACGG TGTGCTGCTG 
TGGGAAGTGG TCAGTTTAGG AGGAACGCCG TACTGCGGGA TGACCTGCGC TGAACTCTAT 
GAGAAGCTGC CGTTGGGCTT CAGGCTGGAG AAACCACTGA ACTGTGACGA CGAGGTTTAT 
GAGCTGATGC AGCAGTGCTG GAGGGAGAAG CCGTTCGAGA GGCCGTCATT CTCTCAGATC 
CTGCTGTCGC TGGGCCGCAT GCTGGAGGAG CGCAAGACAT ATGTCAACAC TACGCTGTAC 
GAGAAGTTCA CCTACGCAGG AATCGACTGC TCCGCGGAGG AGGCCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571536.1
CDS	35..3385
Translation	MCLLDSCTALLLLGCWMSGSAVRISDVTLVNPDPVVSPLTAPSLLCVSSDWSSGGSVLALGQEFPRPQGSVLALGQEFPHTEPRPHPAAATVTWSSRSHAFGAFYCQIRNSTGRKIYTYKMLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHMQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSVTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDQLPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEVVSLGGTPYCGMTCAELYEKLPLGFRLEKPLNCDDEVYELMQQCWREKPFERPSFSQILLSLGRMLEERKTYVNTTLYEKFTYAGIDCSAEEAG

Target ORF information:

RefSeq Version	NM_131461.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131461.1

ORF Insert Sequence:

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCGTCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCAG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCC 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGACACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGCGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACATGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTGCCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TTGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCGTCACAG CGCACGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCAG CTTCCTCCTC CACCGGCCAA CATCTCCATC 
TGGAACATCA GCGATACCTC CGCCGTCCTC ACCTGGGCTG TGGCGGAGGG CGAGTCTGTG 
TCCAGAGCCG TGATCCGCTT CCAGCAGGTG GAGCAGGCGC AGTACAGACA GCAAGTGGAG 
CTCCCGGTGC AGACACAGCA GCTCCACATG CGCTTCCAGC TCCTGGGCCT GAGGCCCAAC 
ACCGGATACC AGCTGCAGCT GTGGACGGTC AATAACATGG GGGAGAGTGC GGAGAGTCCA 
CCGGTCAGCC TGATGACACT GCCACAGCAG GAGAGCTCAG CGCTGTTCGC GGCTCATGGA 
CACCTGCTCC TCTATGCTAT CCTGGGGTCC GCCGGCATGA CCTGCTGCAC CGTCCTGCTG 
GCCTTCTGCA TCGTCCTGCA GCTCAAGAGG AACACACTGC AGCGGCGCAT CCACAGCATA 
CTGAGGGAGG AGCCGGCGGT GCACTTCAGC TCAGCTCCGC CCCCTCATCG GCGCTCGGCG 
GTGGTCAGCC GCTCTCTGGT GTTCCCGGCG CTGCAGTGGA GTGATATCCA GTTCCAGGAC 
GTGCTCGGGG AGGGAAACTT CGGGCAGGTG CTGAAGGCGC GCATCAGGAA GGACGGGCTG 
AGGATGGATG CGGCCGTCAA GAGGATGAAA GATTACGCCT CTCAGGACGA CCACCGCGAC 
TTCGCCGGGG AGCTGGAGGT GCTGTGCAGA CTGGGCCCAC ACAAAAACAT CATACACCTG 
CTGGGAGCCT GCGAACACAG AGGTTACCTG TATCTGGCCA TCGAGTTCGC TCCTCATGGG 
AACCTGCTGG ACTTCCTGCG CAAGAGTCGT GTTCTGGAGA CCGACCCGGC CTTCGCCATC 
GCCCACCGCA CCGCCAGCAC ACTCTCCTCA CAGCAGCTGC TGGCCTTCAG TGCAGACGTG 
GCCCGCGGGA TGAGCTACCT GAGCCAGAAA CAGTTCATAC ACAGAGATCT AGCGGCTCGA 
AACGTCCTGG TGGGAGAGAA CTTTGTGGCG AAGATCGCAG ACTTCGGGCT GTCCAGAGGT 
CAGGAGGTGT ACGTCAAGAA GACCATGGGT CGTCTGCCAG TGCGATGGAT GGCAATAGAG 
TCGCTTAACT ACAGTGTGTA CACCACCAAC AGTGACGTGT GGTCGTACGG TGTGCTGCTG 
TGGGAAGTGG TCAGTTTAGG AGGAACGCCG TACTGCGGGA TGACCTGCGC TGAACTCTAT 
GAGAAGCTGC CGTTGGGCTT CAGGCTGGAG AAACCACTGA ACTGTGACGA CGAGGTTTAT 
GAGCTGATGC AGCAGTGCTG GAGGGAGAAG CCGTTCGAGA GGCCGTCATT CTCTCAGATC 
CTGCTGTCGC TGGGCCGCAT GCTGGAGGAG CGCAAGACAT ATGTCAACAC TACGCTGTAC 
GAGAAGTTCA CCTACGCAGG AATCGACTGC TCCGCGGAGG AGGCCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( XM_009301113.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299388.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.

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CloneID	ODa39325
Clone ID Related Accession (Same CDS sequence)	XM_009301113.3 , XM_009301113.2
Accession Version	XM_009301113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299388.1
CDS	584..3937
Translation	MCLLDSCTALLLLGCWMSGSAVRISDVTLVNPDPVVSPLTAPSLLCVSSDWSSGGSVLALGQEFPRPQGSVLALGQEFPHTEPRPHPAAATVTWSSRSHAFGAFYCQIRNSTGRKIYTYKMLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEVVSLGGTPYCGMTCAELYEKLPLGFRLEKPLNCDDEVYELMQQCWREKPFERPSFSQILLSLGRMLEERKTYVNTTLYEKFTYAGIDCSAEEAG

Target ORF information:

RefSeq Version	XM_009301113.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301113.3

ORF Insert Sequence:

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( XM_009301113.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299388.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.

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CloneID	ODa39325
Clone ID Related Accession (Same CDS sequence)	XM_009301113.3 , XM_009301113.2
Accession Version	XM_009301113.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299388.1
CDS	579..3932
Translation	MCLLDSCTALLLLGCWMSGSAVRISDVTLVNPDPVVSPLTAPSLLCVSSDWSSGGSVLALGQEFPRPQGSVLALGQEFPHTEPRPHPAAATVTWSSRSHAFGAFYCQIRNSTGRKIYTYKMLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEVVSLGGTPYCGMTCAELYEKLPLGFRLEKPLNCDDEVYELMQQCWREKPFERPSFSQILLSLGRMLEERKTYVNTTLYEKFTYAGIDCSAEEAG

Target ORF information:

RefSeq Version	XM_009301113.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301113.2

ORF Insert Sequence:

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGTGGTCGTA CGGTGTGCTG 
CTGTGGGAAG TGGTCAGTTT AGGAGGAACG CCGTACTGCG GGATGACCTG CGCTGAACTC 
TATGAGAAGC TGCCGTTGGG CTTCAGGCTG GAGAAACCAC TGAACTGTGA CGACGAGGTT 
TATGAGCTGA TGCAGCAGTG CTGGAGGGAG AAGCCGTTCG AGAGGCCGTC ATTCTCTCAG 
ATCCTGCTGT CGCTGGGCCG CATGCTGGAG GAGCGCAAGA CATATGTCAA CACTACGCTG 
TACGAGAAGT TCACCTACGC AGGAATCGAC TGCTCCGCGG AGGAGGCCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( XM_009301114.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299389.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.

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CloneID	ODa39326
Clone ID Related Accession (Same CDS sequence)	XM_009301114.2
Accession Version	XM_009301114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGGTCAGAC TCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299389.1
CDS	579..2795
Translation	MCLLDSCTALLLLGCWMSGSAVRISDVTLVNPDPVVSPLTAPSLLCVSSDWSSGGSVLALGQEFPRPQGSVLALGQEFPHTEPRPHPAAATVTWSSRSHAFGAFYCQIRNSTGRKIYTYKMLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSGQTR

Target ORF information:

RefSeq Version	XM_009301114.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301114.2

ORF Insert Sequence:

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGGTCAGAC TCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEK ( XM_017356039.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211528.1 Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59054
Clone ID Related Accession (Same CDS sequence)	XM_017356039.1
Accession Version	XM_017356039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein kinase receptor Tie-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGAGGAACGC CGTACTGCGG 
GATGACCTGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211528.1
CDS	579..3653
Translation	MCLLDSCTALLLLGCWMSGSAVRISDVTLVNPDPVVSPLTAPSLLCVSSDWSSGGSVLALGQEFPRPQGSVLALGQEFPHTEPRPHPAAATVTWSSRSHAFGAFYCQIRNSTGRKIYTYKMLQEAAFLPESLTITVNQGENINISYSRRLYSPEDTVIHKNGHFEHSSPKEDISDIIHYPVTNAKAESHAGIYAIRYISAAPSSAAITRLIVRSCRAGFWGPNCTESCPRCANGGVCDDTTGECVCPPGFRGHTCDIVCGEGRFGAGCKERCVDGVCRALVFCLRDPYGCSCASGWRGLSCNDACPDGYYGAGCTQKCVCAKGRCDRFRGCVCAGRHGSRCEEADSSPVISHLRDVEINTGVELSVNCSASGRPAPLHGDITLITANRTTIAAVDTHTLNDQSTSVFRVQQVRVSSAGRWRCQVNNTHAQVEDEFTVEVKVPPRPQNPPVLQGSGPRHLLLLLNTEPYSGDGPIATTTLLYRPASAHTWSSFTAHGPLVRLDNLYPMTQYLTQVQLSRPGPGGAGQAGPAATFSTQVLELPVGVKLSAVSQTALLLSWDIAPAEQHCTYEVSCLQAGAPGTLRTFQLPSNSSAMHLSDLKPRHKYQCTVRSSCGVGQNHPSASAWTLSDPELPPPPANISIWNISDTSAVLTWAVAEGESVSRAVIRFQQVEQAQYRQQVELPVQTQQLHMRFQLLGLRPNTGYQLQLWTVNNMGESAESPPVSLMTLPQQESSALFAAHGHLLLYAILGSAGMTCCTVLLAFCIVLQLKRNTLQRRIHSILREEPAVHFSSAPPPHRRSAVVSRSLVFPALQWSDIQFQDVLGEGNFGQVLKARIRKDGLRMDAAVKRMKDYASQDDHRDFAGELEVLCRLGPHKNIIHLLGACEHRGYLYLAIEFAPHGNLLDFLRKSRVLETDPAFAIAHRTASTLSSQQLLAFSADVARGMSYLSQKQFIHRDLAARNVLVGENFVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVRNAVLRDDLR

Target ORF information:

RefSeq Version	XM_017356039.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TEK tyrosine kinase, endothelial (tek), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356039.1

ORF Insert Sequence:

ATGTGTCTGC TGGACTCCTG CACTGCTCTG CTGCTGCTCG GCTGCTGGAT GTCAGGCAGT 
GCAGTCCGCA TCTCAGACGT GACTCTGGTG AACCCTGACC CGGTGGTGTC GCCTCTGACC 
GCTCCATCTC TCCTGTGTGT CAGCAGTGAC TGGAGCTCCG GCGGGTCAGT GCTGGCTCTG 
GGTCAGGAGT TCCCCCGTCC ACAGGGGTCA GTGCTGGCTC TGGGTCAGGA GTTCCCCCAT 
ACGGAGCCCC GCCCACACCC CGCCGCCGCT ACCGTCACCT GGAGCTCCCG CAGCCACGCA 
TTCGGGGCCT TCTACTGCCA GATCAGAAAC TCTACTGGCA GAAAAATCTA CACCTACAAG 
ATGCTACAGG AAGCTGCGTT CCTGCCCGAG TCTCTGACCA TCACCGTGAA CCAGGGAGAG 
AACATCAACA TCTCCTACAG CAGGAGGCTG TACTCGCCTG AGGACACGGT CATACATAAA 
AACGGTCATT TCGAACACTC GTCCCCTAAA GAGGACATCT CAGACATCAT CCACTACCCA 
GTGACCAACG CTAAAGCTGA GAGTCACGCG GGAATATATG CTATACGCTA CATCTCTGCA 
GCGCCGTCCA GCGCCGCTAT CACACGGCTG ATCGTCAGGA GCTGTAGAGC GGGTTTCTGG 
GGGCCCAACT GCACTGAGTC GTGTCCGCGC TGCGCTAACG GTGGTGTGTG TGACGATACC 
ACAGGAGAGT GTGTCTGTCC TCCAGGATTT AGAGGACACA CCTGTGACAT AGTGTGTGGC 
GAGGGCCGGT TCGGGGCGGG CTGTAAGGAG CGCTGTGTGG ACGGTGTGTG TCGCGCTCTG 
GTGTTCTGCC TCAGAGACCC GTACGGCTGC TCCTGCGCGT CTGGATGGAG AGGCTTGAGC 
TGCAATGACG CCTGTCCGGA TGGTTATTAC GGCGCTGGGT GTACACAGAA GTGTGTGTGT 
GCAAAAGGCA GGTGTGACCG CTTCAGAGGA TGTGTGTGTG CGGGCCGACA TGGATCTCGC 
TGTGAGGAAG CAGACAGCAG CCCTGTGATC AGCCATCTGA GGGATGTGGA GATCAACACT 
GGTGTGGAGT TGTCAGTGAA CTGCTCTGCG TCCGGCCGAC CCGCTCCACT GCACGGAGAC 
ATCACCCTGA TCACCGCCAA CAGAACCACC ATCGCTGCAG TGGACACACA CACACTGAAT 
GATCAGTCGA CGTCAGTGTT CAGAGTGCAG CAGGTGAGAG TGTCGTCTGC GGGCCGCTGG 
CGCTGTCAGG TGAACAACAC ACACGCGCAG GTGGAGGATG AGTTCACTGT GGAGGTCAAA 
GTACCGCCGC GGCCCCAGAA TCCTCCAGTC CTGCAGGGCA GCGGGCCCCG TCACCTGCTG 
CTGCTGCTCA ACACAGAGCC CTACAGCGGG GACGGACCGA TCGCCACCAC CACACTCCTG 
TACCGGCCTG CCAGCGCACA CACATGGAGC AGCTTCACAG CGCATGGCCC GCTGGTGCGA 
CTGGACAACC TTTACCCCAT GACCCAGTAC CTGACCCAGG TACAGCTCAG CCGCCCCGGC 
CCTGGAGGAG CAGGACAGGC TGGACCTGCA GCCACTTTCT CCACACAGGT GCTCGAGCTG 
CCGGTGGGTG TGAAGCTGTC GGCCGTCAGC CAGACCGCAC TGCTGCTGTC CTGGGACATC 
GCTCCAGCTG AGCAGCACTG CACCTATGAG GTCTCCTGTC TGCAGGCCGG AGCTCCAGGA 
ACACTGAGGA CCTTCCAGCT GCCGTCAAAC TCCTCCGCTA TGCATCTGAG CGATCTGAAA 
CCCAGACACA AATACCAGTG TACAGTGAGG AGCAGCTGCG GCGTGGGACA GAACCATCCC 
TCCGCCAGCG CCTGGACACT CAGCGACCCA GAGCTTCCTC CTCCACCGGC CAACATCTCC 
ATCTGGAACA TCAGCGATAC CTCCGCCGTC CTCACCTGGG CTGTGGCGGA GGGCGAGTCT 
GTGTCCAGAG CCGTGATCCG CTTCCAGCAG GTGGAGCAGG CGCAGTACAG ACAGCAAGTG 
GAGCTCCCGG TGCAGACACA GCAGCTCCAC ATGCGCTTCC AGCTCCTGGG CCTGAGGCCC 
AACACCGGAT ACCAGCTGCA GCTGTGGACG GTCAATAACA TGGGGGAGAG TGCGGAGAGT 
CCACCGGTCA GCCTGATGAC ACTGCCACAG CAGGAGAGCT CAGCGCTGTT CGCGGCTCAT 
GGACACCTGC TCCTCTATGC TATCCTGGGG TCCGCCGGCA TGACCTGCTG CACCGTCCTG 
CTGGCCTTCT GCATCGTCCT GCAGCTCAAG AGGAACACAC TGCAGCGGCG CATCCACAGC 
ATACTGAGGG AGGAGCCGGC GGTGCACTTC AGCTCAGCTC CGCCCCCTCA CAGGCGCTCG 
GCGGTGGTCA GCCGCTCTCT GGTGTTCCCG GCGCTGCAGT GGAGTGATAT CCAGTTCCAG 
GACGTGCTCG GGGAGGGAAA CTTCGGGCAG GTGCTGAAGG CGCGCATCAG GAAGGACGGG 
CTGAGGATGG ATGCGGCCGT CAAGAGGATG AAAGATTACG CCTCTCAGGA CGACCACCGC 
GACTTCGCCG GGGAGCTGGA GGTGCTGTGC AGACTGGGCC CACACAAAAA CATCATACAC 
CTGCTGGGAG CCTGCGAACA CAGAGGTTAC CTGTATCTGG CCATCGAGTT TGCTCCTCAT 
GGGAACCTGC TGGACTTCCT GCGCAAGAGT CGTGTTCTGG AGACCGACCC GGCCTTCGCC 
ATCGCCCACC GCACCGCCAG CACACTCTCC TCACAGCAGC TGCTGGCCTT CAGTGCAGAC 
GTGGCCCGCG GGATGAGCTA TCTGAGCCAG AAACAGTTCA TACACAGAGA TCTAGCGGCT 
CGAAACGTCC TGGTGGGAGA GAACTTTGTG GCGAAGATCG CAGACTTCGG GCTGTCCAGA 
GGTCAGGAGG TGTACGTCAA GAAGACCATG GGTCGTCTGC CAGTGCGATG GATGGCAATA 
GAGTCGCTTA ACTACAGTGT GTACACCACC AACAGTGACG TGAGGAACGC CGTACTGCGG 
GATGACCTGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Etv2-miR-130a-Jarid2 cascade regulates vascular patterning during embryogenesis.
PloS one12(12)e0189010(2017)
Singh BN,Tahara N,Kawakami Y,Das S,Koyano-Nakagawa N,Gong W,Garry MG,Garry DJ

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TEK cDNA ORF clone, Danio rerio(Zebrafish)

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