melk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	melk
Entrez Gene ID	30724
Full Name	maternal embryonic leucine zipper kinase
Synonyms	fb71b01,fe01f04,wu:fb71b01,wu:fe01f04
General protein information	Preferred Names maternal embryonic leucine zipper kinase Names maternal embryonic leucine zipper kinase protein kinase PK38 zMelk
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_206888.2	NP_996771.2	maternal embryonic leucine zipper kinase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MELK	XP_538730.2
Gallus gallus (chicken)	MELK	NP_001026680.1
Xenopus tropicalis (tropical clawed frog)	melk	NP_001016390.1
Rattus norvegicus (Norway rat)	Melk	NP_001102132.1
Pan troglodytes (chimpanzee)	MELK	XP_001169038.1
Mus musculus (house mouse)	Melk	NP_034920.2
Danio rerio (zebrafish)	melk	NP_996771.2
Arabidopsis thaliana (thale cress)	CIPK7	NP_188940.1
Homo sapiens (human)	MELK	NP_055606.1
	MELK	XP_001115076.2
Bos taurus (cattle)	MELK	NP_001104730.1
Caenorhabditis elegans (roundworm)	pig-1	NP_001023420.2
Arabidopsis thaliana (thale cress)	CIPK4	NP_193194.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

Normalization of RNA-sequencing data from samples with varying mRNA levels.
Aanes H, Winata C, Moen LF, ?strup O, Mathavan S, Collas P, Rognes T, Alestr?m P
PloS one9(2)e89158(2014)

Toxicological assessment of trace ?-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
Yin X, Wang H, Zhang Y, Dahlgren RA, Zhang H, Shi M, Gao M, Wang X
PloS one9(7)e102731(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Melk-like gene may play a role in primitive hematopoiesis by affecting the expression of genes critical for hematopoiesis.
Title: Melk-like kinase plays a role in hematopoiesis in the zebra fish.

The following melk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the melk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37363	NM_206888.2 Latest version!	Danio rerio maternal embryonic leucine zipper kinase (melk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

melk ( NM_206888.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996771.2 Danio rerio maternal embryonic leucine zipper kinase (melk), mRNA.

Price & Availability↑

CloneID	ODa37363
Clone ID Related Accession (Same CDS sequence)	NM_206888.2
Accession Version	NM_206888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Danio rerio(zebrafish)
Product	maternal embryonic leucine zipper kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050520.1. On Apr 5, 2004 this sequence version replaced NM_206888.1. ##Evidence-Data-START## Transcript exon combination :: BC050520.1, AB108827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGTAG ACAGCACTTC TGAACTCCTC AGGCACTATG AGGTTTATGA AACCATTGGC 
TCAGGTGGGT TTGCCAAGGT CAAACTTGGC AGACACAAAC TGACAGGAGA GAAAGTAGCC 
ATCAAAATAA TGGAGAAAAA AGATCTTGGG GATGATCTGC CCAGAGTAAA GATAGAGATT 
GAGGCAATGA AGAACCTCAG TCATCAACAT GTATGTCGTC TCTACCATGT CATTGAGACC 
ACAAGCAAAA TCTACATGGT GCTTGAGTAC TGTCCTGGAG GAGAACTCTT TGATTACATC 
ATTGCTAAAG ATCGGTTATC AGAGGAAGAG ACCCGAGTGT TTTTTCGCCA GATCATCTCT 
GCGTTGGCCT ATGTTCATAG CCAAGGTTAT GCCCATCGAG ACCTCAAACC TGAAAACTTG 
CTGATCGATG AAGATCATAA CCTAAAACTC ATTGATTTTG GCCTGTGTGC CAAGCCAAAG 
GGTGGTTTGG GTTTTGAATT GCTGACATGT TGTGGCAGTC CTGCATACGC AGCTCCAGAG 
CTCATTCAGG GCAAGGCTTA CATCGGCTCT GAGGCTGATG TTTGGAGTAT GGGTGTATTG 
CTTTATGCAC TACTGTGTGG TTTTCTACCA TTTGATGATG ACAATTGTAT GGTTCTCTAT 
AGAAAAATCA CAAGAGGCAA ATACAGCAAC CCACACTGGC TTTCACCTTC TAGTATTCTG 
CTTCTAAACC AAATGATGCA GGTGGACCCA AAGAGGCGTT TGACAGTGAA ACATTTGCTT 
GATCACCCAT GGGCTATGAG GGGCTACAGC ACACCAGTGG AGTGGCACAG TAAATATCCA 
TTGGGTCATA TAGATGAAGA CTGCATCACA GAAATGGCGG TGACTTTCAA ACAGTCCAAA 
CAACGAACTA TCCAGCTTGT GTCTGAGTGG AAGTATGATC AAATAACAGC AACCTACCTT 
TTACTGCTGG CTAAGAAGCG TCAAGGCCGT CCTGTGCGTC TGAGAGCTGA ATGCCCTGTG 
ATTGACATTG TGTGTTCTCC TTTACAGGAT ATGCAACTGA AGAAGAGTCT ACGCTTCACT 
GAGGATGATG ATGGTGTTCA CCCAGTGGTG CTCGGCTCTA TGGTCTTTCC CCCTGATTGC 
TACGATGACG AGAATCCCTG GACCCCTCTC ACACCCAAGA ACACACATAC CACAAACACT 
CCACGCATGA AGCTGTACCC TGAAACAACA GAGAAGTGGA ATGAAATGGC TTACTCCCCC 
GTCATAGAGC ACAGCAGACC CTGCCGACAG AAGCCAGAGA GACGTGACAG AACCAAGGAA 
AATAAAGAGA ACCTGGCAGT GCCAGGCACA GATGGAGATG TTTTTGCTCT GCCAGCGCCG 
CGAACTCCAA CATCCAGCCG AAAGGTCAAA TCCAACAGAA CTGTAATGAC AACGCCCAAC 
CACAACAACA ACAAGAGCAG CGAAGTAAAC AAAGGAGCGG GCAGTGCCAC TAAAGAAGGG 
TCCCGCAGGA GAGAGGTGGA ACAGCAGCAG AACGGCCAAC AAGGAGAACT CAATATGTTG 
GCTTTTAGCC CTGAGCGAAG GTCTCGATCA CTGGACCTGG CAGGCTGTCA GGTGGACAGT 
GGGCAGAAGC GTAAGGGAGG GAAAGTGTTT GGCAGTCTGG AAAGAGGCCT GGATAAAGTC 
ATTACAATGC TTACACCCAG TAAAAAGAGA GGACCTCGAG ATGGCCCACG CAAAATTAAG 
GCTCAGTATA ATGTAACTCT CACCAATCAA ACGAATGCTG ACCAAGTGCT CAATCAGATT 
CTCAGCATAT TACCAGAGAA GAATGTGGAC TTTGTTCAGA AGGGCTATAC TCTGAAATGC 
CACACGCAGT CAGACTTTGG GAAGGTCACC ATGCAGTTCG AGCTGGAGGT TTGTCTCCTG 
CAGAAGCCTG AGGTTGTGGG GATCCGTCGA CAACGGCTCA AAGGAGATGC CTGGGTGTAT 
AAACACCCGG TAGAGGACAT TCTGTCCTCC TCCAGCCAAT GGTCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996771.2
CDS	224..2254
Translation	MPVDSTSELLRHYEVYETIGSGGFAKVKLGRHKLTGEKVAIKIMEKKDLGDDLPRVKIEIEAMKNLSHQHVCRLYHVIETTSKIYMVLEYCPGGELFDYIIAKDRLSEEETRVFFRQIISALAYVHSQGYAHRDLKPENLLIDEDHNLKLIDFGLCAKPKGGLGFELLTCCGSPAYAAPELIQGKAYIGSEADVWSMGVLLYALLCGFLPFDDDNCMVLYRKITRGKYSNPHWLSPSSILLLNQMMQVDPKRRLTVKHLLDHPWAMRGYSTPVEWHSKYPLGHIDEDCITEMAVTFKQSKQRTIQLVSEWKYDQITATYLLLLAKKRQGRPVRLRAECPVIDIVCSPLQDMQLKKSLRFTEDDDGVHPVVLGSMVFPPDCYDDENPWTPLTPKNTHTTNTPRMKLYPETTEKWNEMAYSPVIEHSRPCRQKPERRDRTKENKENLAVPGTDGDVFALPAPRTPTSSRKVKSNRTVMTTPNHNNNKSSEVNKGAGSATKEGSRRREVEQQQNGQQGELNMLAFSPERRSRSLDLAGCQVDSGQKRKGGKVFGSLERGLDKVITMLTPSKKRGPRDGPRKIKAQYNVTLTNQTNADQVLNQILSILPEKNVDFVQKGYTLKCHTQSDFGKVTMQFELEVCLLQKPEVVGIRRQRLKGDAWVYKHPVEDILSSSSQWSA

Target ORF information:

RefSeq Version	NM_206888.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio maternal embryonic leucine zipper kinase (melk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206888.2

ORF Insert Sequence:

ATGCCAGTAG ACAGCACTTC TGAACTCCTC AGGCACTATG AGGTTTATGA AACCATTGGC 
TCAGGTGGGT TTGCCAAGGT CAAACTTGGC AGACACAAAC TGACAGGAGA GAAAGTAGCC 
ATCAAAATAA TGGAGAAAAA AGATCTTGGG GATGATCTGC CCAGAGTAAA GATAGAGATT 
GAGGCAATGA AGAACCTCAG TCATCAACAT GTATGTCGTC TCTACCATGT CATTGAGACC 
ACAAGCAAAA TCTACATGGT GCTTGAGTAC TGTCCTGGAG GAGAACTCTT TGATTACATC 
ATTGCTAAAG ATCGGTTATC AGAGGAAGAG ACCCGAGTGT TTTTTCGCCA GATCATCTCT 
GCGTTGGCCT ATGTTCATAG CCAAGGTTAT GCCCATCGAG ACCTCAAACC TGAAAACTTG 
CTGATCGATG AAGATCATAA CCTAAAACTC ATTGATTTTG GCCTGTGTGC CAAGCCAAAG 
GGTGGTTTGG GTTTTGAATT GCTGACATGT TGTGGCAGTC CTGCATACGC AGCTCCAGAG 
CTCATTCAGG GCAAGGCTTA CATCGGCTCT GAGGCTGATG TTTGGAGTAT GGGTGTATTG 
CTTTATGCAC TACTGTGTGG TTTTCTACCA TTTGATGATG ACAATTGTAT GGTTCTCTAT 
AGAAAAATCA CAAGAGGCAA ATACAGCAAC CCACACTGGC TTTCACCTTC TAGTATTCTG 
CTTCTAAACC AAATGATGCA GGTGGACCCA AAGAGGCGTT TGACAGTGAA ACATTTGCTT 
GATCACCCAT GGGCTATGAG GGGCTACAGC ACACCAGTGG AGTGGCACAG TAAATATCCA 
TTGGGTCATA TAGATGAAGA CTGCATCACA GAAATGGCGG TGACTTTCAA ACAGTCCAAA 
CAACGAACTA TCCAGCTTGT GTCTGAGTGG AAGTATGATC AAATAACAGC AACCTACCTT 
TTACTGCTGG CTAAGAAGCG TCAAGGCCGT CCTGTGCGTC TGAGAGCTGA ATGCCCTGTG 
ATTGACATTG TGTGTTCTCC TTTACAGGAT ATGCAACTGA AGAAGAGTCT ACGCTTCACT 
GAGGATGATG ATGGTGTTCA CCCAGTGGTG CTCGGCTCTA TGGTCTTTCC CCCTGATTGC 
TACGATGACG AGAATCCCTG GACCCCTCTC ACACCCAAGA ACACACATAC CACAAACACT 
CCACGCATGA AGCTGTACCC TGAAACAACA GAGAAGTGGA ATGAAATGGC TTACTCCCCC 
GTCATAGAGC ACAGCAGACC CTGCCGACAG AAGCCAGAGA GACGTGACAG AACCAAGGAA 
AATAAAGAGA ACCTGGCAGT GCCAGGCACA GATGGAGATG TTTTTGCTCT GCCAGCGCCG 
CGAACTCCAA CATCCAGCCG AAAGGTCAAA TCCAACAGAA CTGTAATGAC AACGCCCAAC 
CACAACAACA ACAAGAGCAG CGAAGTAAAC AAAGGAGCGG GCAGTGCCAC TAAAGAAGGG 
TCCCGCAGGA GAGAGGTGGA ACAGCAGCAG AACGGCCAAC AAGGAGAACT CAATATGTTG 
GCTTTTAGCC CTGAGCGAAG GTCTCGATCA CTGGACCTGG CAGGCTGTCA GGTGGACAGT 
GGGCAGAAGC GTAAGGGAGG GAAAGTGTTT GGCAGTCTGG AAAGAGGCCT GGATAAAGTC 
ATTACAATGC TTACACCCAG TAAAAAGAGA GGACCTCGAG ATGGCCCACG CAAAATTAAG 
GCTCAGTATA ATGTAACTCT CACCAATCAA ACGAATGCTG ACCAAGTGCT CAATCAGATT 
CTCAGCATAT TACCAGAGAA GAATGTGGAC TTTGTTCAGA AGGGCTATAC TCTGAAATGC 
CACACGCAGT CAGACTTTGG GAAGGTCACC ATGCAGTTCG AGCTGGAGGT TTGTCTCCTG 
CAGAAGCCTG AGGTTGTGGG GATCCGTCGA CAACGGCTCA AAGGAGATGC CTGGGTGTAT 
AAACACCCGG TAGAGGACAT TCTGTCCTCC TCCAGCCAAT GGTCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ

Normalization of RNA-sequencing data from samples with varying mRNA levels.
PloS one9(2)e89158(2014)
Aanes H,Winata C,Moen LF,?strup O,Mathavan S,Collas P,Rognes T,Alestr?m P

Toxicological assessment of trace ?-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
PloS one9(7)e102731(2014)
Yin X,Wang H,Zhang Y,Dahlgren RA,Zhang H,Shi M,Gao M,Wang X

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melk cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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