smad2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smad2
Entrez Gene ID	30639
Full Name	SMAD family member 2
Synonyms	fj43c06,madh2,wu:fj43c06
General protein information	Preferred Names SMAD family member 2 Names mothers against decapentaplegic homolog 2 MAD homolog 2 SMA- and MAD-related protein 2 mothers against DPP homolog 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290015.1	NP_001276944.1	mothers against decapentaplegic homolog 2
NM_131366.3	NP_571441.3	mothers against decapentaplegic homolog 2
XM_021479107.1	XP_021334782.1	mothers against decapentaplegic homolog 2 isoform X2
XM_005155558.3	XP_005155615.1	mothers against decapentaplegic homolog 2 isoform X1
XM_005155558.4	XP_005155615.1	mothers against decapentaplegic homolog 2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smad2	NP_571441.2
Pan troglodytes (chimpanzee)	SMAD2	XP_001149585.1
Gallus gallus (chicken)	SMAD2	NP_989892.1
	SMAD2	XP_001086616.1
Mus musculus (house mouse)	Smad2	NP_034884.2
Rattus norvegicus (Norway rat)	Smad2	NP_062064.1
Canis lupus familiaris (dog)	SMAD2	XP_005622887.1
Bos taurus (cattle)	SMAD2	NP_001039683.1
Xenopus tropicalis (tropical clawed frog)	smad2	NP_001011168.1
Homo sapiens (human)	SMAD2	NP_001003652.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification.
Hozumi S, Aoki S, Kikuchi Y
Development (Cambridge, England)144(21)4015-4025(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The non-Smad JNK signaling pathway, which is downstream of Nodal signaling, regulates nuclear movement independently of the Smad pathway, and this nuclear movement is associated with Smad signal transduction toward the nucleus.
Title: Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification.

The results of this study found that Bptf and TGF-beta/Smad2 mediate nucleosome remodeling to regulate wnt8a expression and hence neural posteriorization.
Title: The Chromatin Remodeling Protein Bptf Promotes Posterior Neuroectodermal Fate by Enhancing Smad2-Activated wnt8a Expression.

Smad2 and Eomesodermin a (Eomesa) bind common genomic regions proximal to genes involved in mesoderm and endoderm formation, suggesting Eomesa forms a general component of the Smad2 signalling complex in zebrafish.
Title: Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression.

study systemically uncovers a large number of Smad2 targets in early gastrulas and suggests cooperative roles of Smad2 and other transcription factors in controlling target gene transcription
Title: Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.

Smad2/3 activities play important roles not only in mesendodermal development but also in neural development during early vertebrate embryogenesis
Title: Smad2/3 activities are required for induction and patterning of the neuroectoderm in zebrafish.

The following smad2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smad2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02834	NM_131366.3 Latest version!	Danio rerio SMAD family member 2 (smad2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa02834	NM_001290015.1 Latest version!	Danio rerio SMAD family member 2 (smad2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa15886	XM_005155558.4 Latest version!	Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa147585	XM_021479107.1 Latest version!	Danio rerio SMAD family member 2 (smad2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa15886	XM_005155558.3	Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smad2 ( NM_001290015.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001276944.1 Danio rerio SMAD family member 2 (smad2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa02834
Clone ID Related Accession (Same CDS sequence)	NM_001290015.1 , NM_131366.3
Accession Version	NM_001290015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR536613.20 and CU138537.5. On Feb 20, 2014 this sequence version replaced XM_005155557.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01038975.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTATGGACA CAGGTTCTCC TGCAGAACTG TCACCAAGCA CACTCTCGCC TGTCAATCAT 
GGCATGGACC TGCAGCCAGT GACTTACTCA GAGCCTGCGT TTTGGTGCTC CATAGCTTAC 
TATGAACTTA ACCAGCGGGT TGGAGAAACA TTCCACGCCT CTCAGCCTTC CCTCACCGTG 
GACGGCTTCA CAGACCCCTC CAATTCAGAG CGATTCTGCC TGGGCTTGCT GTCCAACGTC 
AACCGCAACG CCACCGTCGA GATGACCCGA AGACATATAG GACGAGGAGT CAGGCTGTAT 
TATATTGGTG GGGAAGTGTT TGCTGAATGT CTTAGCGATA GCGCCATCTT TGTTCAGAGC 
CCGAACTGTA ATCAGAGGTA TGGCTGGCAT CCTGCAACAG TCTGTAAAAT CCCCCCAGGC 
TGTAACCTGA AGATCTTCAA CAACCAGGAG TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC 
CAGGGGTTCG AGGCTGTTTA TCAGTTGACC AGGATGTGCA CCATTCGCAT GAGTTTTGTC 
AAAGGCTGGG GAGCAGAGTA CAGACGGCAG ACAGTGACGA GCACCCCCTG CTGGATCGAG 
CTGCATCTGA ACGGGCCCCT CCAGTGGCTG GACAAGGTTC TGACCCAGAT GGGCTCTCCC 
TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276944.1
CDS	435..1841
Translation	MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNRNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTNSIPENTNFPTGIEPPNNYIPETPPPGYISEDGEASDQQMNQSMDTGSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Target ORF information:

RefSeq Version	NM_001290015.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 2 (smad2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290015.1

ORF Insert Sequence:

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTATGGACA CAGGTTCTCC TGCAGAACTG TCACCAAGCA CACTCTCGCC TGTCAATCAT 
GGCATGGACC TGCAGCCAGT GACTTACTCA GAGCCTGCGT TTTGGTGCTC CATAGCTTAC 
TATGAACTTA ACCAGCGGGT TGGAGAAACA TTCCACGCCT CTCAGCCTTC CCTCACCGTG 
GACGGCTTCA CAGACCCCTC CAATTCAGAG CGATTCTGCC TGGGCTTGCT GTCCAACGTC 
AACCGCAACG CCACCGTCGA GATGACCCGA AGACATATAG GACGAGGAGT CAGGCTGTAT 
TATATTGGTG GGGAAGTGTT TGCTGAATGT CTTAGCGATA GCGCCATCTT TGTTCAGAGC 
CCGAACTGTA ATCAGAGGTA TGGCTGGCAT CCTGCAACAG TCTGTAAAAT CCCCCCAGGC 
TGTAACCTGA AGATCTTCAA CAACCAGGAG TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC 
CAGGGGTTCG AGGCTGTTTA TCAGTTGACC AGGATGTGCA CCATTCGCAT GAGTTTTGTC 
AAAGGCTGGG GAGCAGAGTA CAGACGGCAG ACAGTGACGA GCACCCCCTG CTGGATCGAG 
CTGCATCTGA ACGGGCCCCT CCAGTGGCTG GACAAGGTTC TGACCCAGAT GGGCTCTCCC 
TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smad2 ( NM_131366.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571441.3 Danio rerio SMAD family member 2 (smad2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa02834
Clone ID Related Accession (Same CDS sequence)	NM_001290015.1 , NM_131366.3
Accession Version	NM_131366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR536613.20 and CU138537.5. On Feb 19, 2014 this sequence version replaced NM_131366.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044338.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTATGGACA CAGGTTCTCC TGCAGAACTG TCACCAAGCA CACTCTCGCC TGTCAATCAT 
GGCATGGACC TGCAGCCAGT GACTTACTCA GAGCCTGCGT TTTGGTGCTC CATAGCTTAC 
TATGAACTTA ACCAGCGGGT TGGAGAAACA TTCCACGCCT CTCAGCCTTC CCTCACCGTG 
GACGGCTTCA CAGACCCCTC CAATTCAGAG CGATTCTGCC TGGGCTTGCT GTCCAACGTC 
AACCGCAACG CCACCGTCGA GATGACCCGA AGACATATAG GACGAGGAGT CAGGCTGTAT 
TATATTGGTG GGGAAGTGTT TGCTGAATGT CTTAGCGATA GCGCCATCTT TGTTCAGAGC 
CCGAACTGTA ATCAGAGGTA TGGCTGGCAT CCTGCAACAG TCTGTAAAAT CCCCCCAGGC 
TGTAACCTGA AGATCTTCAA CAACCAGGAG TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC 
CAGGGGTTCG AGGCTGTTTA TCAGTTGACC AGGATGTGCA CCATTCGCAT GAGTTTTGTC 
AAAGGCTGGG GAGCAGAGTA CAGACGGCAG ACAGTGACGA GCACCCCCTG CTGGATCGAG 
CTGCATCTGA ACGGGCCCCT CCAGTGGCTG GACAAGGTTC TGACCCAGAT GGGCTCTCCC 
TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571441.3
CDS	356..1762
Translation	MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNRNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTNSIPENTNFPTGIEPPNNYIPETPPPGYISEDGEASDQQMNQSMDTGSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Target ORF information:

RefSeq Version	NM_131366.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 2 (smad2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131366.3

ORF Insert Sequence:

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTATGGACA CAGGTTCTCC TGCAGAACTG TCACCAAGCA CACTCTCGCC TGTCAATCAT 
GGCATGGACC TGCAGCCAGT GACTTACTCA GAGCCTGCGT TTTGGTGCTC CATAGCTTAC 
TATGAACTTA ACCAGCGGGT TGGAGAAACA TTCCACGCCT CTCAGCCTTC CCTCACCGTG 
GACGGCTTCA CAGACCCCTC CAATTCAGAG CGATTCTGCC TGGGCTTGCT GTCCAACGTC 
AACCGCAACG CCACCGTCGA GATGACCCGA AGACATATAG GACGAGGAGT CAGGCTGTAT 
TATATTGGTG GGGAAGTGTT TGCTGAATGT CTTAGCGATA GCGCCATCTT TGTTCAGAGC 
CCGAACTGTA ATCAGAGGTA TGGCTGGCAT CCTGCAACAG TCTGTAAAAT CCCCCCAGGC 
TGTAACCTGA AGATCTTCAA CAACCAGGAG TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC 
CAGGGGTTCG AGGCTGTTTA TCAGTTGACC AGGATGTGCA CCATTCGCAT GAGTTTTGTC 
AAAGGCTGGG GAGCAGAGTA CAGACGGCAG ACAGTGACGA GCACCCCCTG CTGGATCGAG 
CTGCATCTGA ACGGGCCCCT CCAGTGGCTG GACAAGGTTC TGACCCAGAT GGGCTCTCCC 
TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smad2 ( XM_005155558.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155615.1 Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15886
Clone ID Related Accession (Same CDS sequence)	XM_005155558.3 , XM_005155558.4
Accession Version	XM_005155558.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mothers against decapentaplegic homolog 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTTCTCCTG CAGAACTGTC ACCAAGCACA CTCTCGCCTG TCAATCATGG CATGGACCTG 
CAGCCAGTGA CTTACTCAGA GCCTGCGTTT TGGTGCTCCA TAGCTTACTA TGAACTTAAC 
CAGCGGGTTG GAGAAACATT CCACGCCTCT CAGCCTTCCC TCACCGTGGA CGGCTTCACA 
GACCCCTCCA ATTCAGAGCG ATTCTGCCTG GGCTTGCTGT CCAACGTCAA CCGCAACGCC 
ACCGTCGAGA TGACCCGAAG ACATATAGGA CGAGGAGTCA GGCTGTATTA TATTGGTGGG 
GAAGTGTTTG CTGAATGTCT TAGCGATAGC GCCATCTTTG TTCAGAGCCC GAACTGTAAT 
CAGAGGTATG GCTGGCATCC TGCAACAGTC TGTAAAATCC CCCCAGGCTG TAACCTGAAG 
ATCTTCAACA ACCAGGAGTT TGCAGCATTG CTGGCTCAGT CGGTGAACCA GGGGTTCGAG 
GCTGTTTATC AGTTGACCAG GATGTGCACC ATTCGCATGA GTTTTGTCAA AGGCTGGGGA 
GCAGAGTACA GACGGCAGAC AGTGACGAGC ACCCCCTGCT GGATCGAGCT GCATCTGAAC 
GGGCCCCTCC AGTGGCTGGA CAAGGTTCTG ACCCAGATGG GCTCTCCCTC CGTACGCTGC 
TCCAGTATGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155615.1
CDS	423..1817
Translation	MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNRNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTNSIPENTNFPTGIEPPNNYIPETPPPGYISEDGEASDQQMNQSSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Target ORF information:

RefSeq Version	XM_005155558.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155558.3

ORF Insert Sequence:

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTTCTCCTG CAGAACTGTC ACCAAGCACA CTCTCGCCTG TCAATCATGG CATGGACCTG 
CAGCCAGTGA CTTACTCAGA GCCTGCGTTT TGGTGCTCCA TAGCTTACTA TGAACTTAAC 
CAGCGGGTTG GAGAAACATT CCACGCCTCT CAGCCTTCCC TCACCGTGGA CGGCTTCACA 
GACCCCTCCA ATTCAGAGCG ATTCTGCCTG GGCTTGCTGT CCAACGTCAA CCGCAACGCC 
ACCGTCGAGA TGACCCGAAG ACATATAGGA CGAGGAGTCA GGCTGTATTA TATTGGTGGG 
GAAGTGTTTG CTGAATGTCT TAGCGATAGC GCCATCTTTG TTCAGAGCCC GAACTGTAAT 
CAGAGGTATG GCTGGCATCC TGCAACAGTC TGTAAAATCC CCCCAGGCTG TAACCTGAAG 
ATCTTCAACA ACCAGGAGTT TGCAGCATTG CTGGCTCAGT CGGTGAACCA GGGGTTCGAG 
GCTGTTTATC AGTTGACCAG GATGTGCACC ATTCGCATGA GTTTTGTCAA AGGCTGGGGA 
GCAGAGTACA GACGGCAGAC AGTGACGAGC ACCCCCTGCT GGATCGAGCT GCATCTGAAC 
GGGCCCCTCC AGTGGCTGGA CAAGGTTCTG ACCCAGATGG GCTCTCCCTC CGTACGCTGC 
TCCAGTATGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smad2 ( XM_005155558.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155615.1 Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.

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CloneID	ODa15886
Clone ID Related Accession (Same CDS sequence)	XM_005155558.3 , XM_005155558.4
Accession Version	XM_005155558.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155558.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTTCTCCTG CAGAACTGTC ACCAAGCACA CTCTCGCCTG TCAATCATGG CATGGACCTG 
CAGCCAGTGA CTTACTCAGA GCCTGCGTTT TGGTGCTCCA TAGCTTACTA TGAACTTAAC 
CAGCGGGTTG GAGAAACATT CCACGCCTCT CAGCCTTCCC TCACCGTGGA CGGCTTCACA 
GACCCCTCCA ATTCAGAGCG ATTCTGCCTG GGCTTGCTGT CCAACGTCAA CCGCAACGCC 
ACCGTCGAGA TGACCCGAAG ACATATAGGA CGAGGAGTCA GGCTGTATTA TATTGGTGGG 
GAAGTGTTTG CTGAATGTCT TAGCGATAGC GCCATCTTTG TTCAGAGCCC GAACTGTAAT 
CAGAGGTATG GCTGGCATCC TGCAACAGTC TGTAAAATCC CCCCAGGCTG TAACCTGAAG 
ATCTTCAACA ACCAGGAGTT TGCAGCATTG CTGGCTCAGT CGGTGAACCA GGGGTTCGAG 
GCTGTTTATC AGTTGACCAG GATGTGCACC ATTCGCATGA GTTTTGTCAA AGGCTGGGGA 
GCAGAGTACA GACGGCAGAC AGTGACGAGC ACCCCCTGCT GGATCGAGCT GCATCTGAAC 
GGGCCCCTCC AGTGGCTGGA CAAGGTTCTG ACCCAGATGG GCTCTCCCTC CGTACGCTGC 
TCCAGTATGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155615.1
CDS	435..1829
Translation	MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNRNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTNSIPENTNFPTGIEPPNNYIPETPPPGYISEDGEASDQQMNQSSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Target ORF information:

RefSeq Version	XM_005155558.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 2 (smad2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155558.4

ORF Insert Sequence:

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGC 
AATTGCTCTG AAATTTGGGG ACTGAGTACA CCAAATACGA TAGAACAGTG GGATACCTCA 
GGCCTTTACA GCTACCCTGA CCAAACCAGA TCTCTGGACG GGCGTTTGCA AGTGTCTCAC 
CGTAAAGGTC TGCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCCGA CCTGCACAGC 
CATCACGAGC TGCGCGCCAT CGAGACCTGC GAGTATGCTT TCAACCTCAA GAAGGATGAA 
GTCTGCGTCA ATCCCTACCA CTACCAGCGG GTGGAGACAC CAGTTCTTCC TCCTGTTCTC 
GTGCCAAGAC ACACGGAGAT CCTGACTGAG TTGCCACCTT TGGACGACTA CACCAACTCC 
ATACCTGAAA ACACCAACTT CCCAACAGGG ATCGAGCCCC CCAACAATTA CATACCAGAA 
ACTCCTCCAC CGGGATACAT CAGTGAGGAT GGGGAGGCCA GTGACCAGCA AATGAATCAA 
AGTTCTCCTG CAGAACTGTC ACCAAGCACA CTCTCGCCTG TCAATCATGG CATGGACCTG 
CAGCCAGTGA CTTACTCAGA GCCTGCGTTT TGGTGCTCCA TAGCTTACTA TGAACTTAAC 
CAGCGGGTTG GAGAAACATT CCACGCCTCT CAGCCTTCCC TCACCGTGGA CGGCTTCACA 
GACCCCTCCA ATTCAGAGCG ATTCTGCCTG GGCTTGCTGT CCAACGTCAA CCGCAACGCC 
ACCGTCGAGA TGACCCGAAG ACATATAGGA CGAGGAGTCA GGCTGTATTA TATTGGTGGG 
GAAGTGTTTG CTGAATGTCT TAGCGATAGC GCCATCTTTG TTCAGAGCCC GAACTGTAAT 
CAGAGGTATG GCTGGCATCC TGCAACAGTC TGTAAAATCC CCCCAGGCTG TAACCTGAAG 
ATCTTCAACA ACCAGGAGTT TGCAGCATTG CTGGCTCAGT CGGTGAACCA GGGGTTCGAG 
GCTGTTTATC AGTTGACCAG GATGTGCACC ATTCGCATGA GTTTTGTCAA AGGCTGGGGA 
GCAGAGTACA GACGGCAGAC AGTGACGAGC ACCCCCTGCT GGATCGAGCT GCATCTGAAC 
GGGCCCCTCC AGTGGCTGGA CAAGGTTCTG ACCCAGATGG GCTCTCCCTC CGTACGCTGC 
TCCAGTATGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smad2 ( XM_021479107.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334782.1 Danio rerio SMAD family member 2 (smad2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147585
Clone ID Related Accession (Same CDS sequence)	XM_021479107.1
Accession Version	XM_021479107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGA 
TCTCTGGACG GGCGTTTGCA AGTGTCTCAC CGTAAAGGTC TGCCTCATGT CATCTACTGC 
CGCCTGTGGC GATGGCCCGA CCTGCACAGC CATCACGAGC TGCGCGCCAT CGAGACCTGC 
GAGTATGCTT TCAACCTCAA GAAGGATGAA GTCTGCGTCA ATCCCTACCA CTACCAGCGG 
GTGGAGACAC CAGTTCTTCC TCCTGTTCTC GTGCCAAGAC ACACGGAGAT CCTGACTGAG 
TTGCCACCTT TGGACGACTA CACCAACTCC ATACCTGAAA ACACCAACTT CCCAACAGGG 
ATCGAGCCCC CCAACAATTA CATACCAGAA ACTCCTCCAC CGGGATACAT CAGTGAGGAT 
GGGGAGGCCA GTGACCAGCA AATGAATCAA AGTATGGACA CAGGTTCTCC TGCAGAACTG 
TCACCAAGCA CACTCTCGCC TGTCAATCAT GGCATGGACC TGCAGCCAGT GACTTACTCA 
GAGCCTGCGT TTTGGTGCTC CATAGCTTAC TATGAACTTA ACCAGCGGGT TGGAGAAACA 
TTCCACGCCT CTCAGCCTTC CCTCACCGTG GACGGCTTCA CAGACCCCTC CAATTCAGAG 
CGATTCTGCC TGGGCTTGCT GTCCAACGTC AACCGCAACG CCACCGTCGA GATGACCCGA 
AGACATATAG GACGAGGAGT CAGGCTGTAT TATATTGGTG GGGAAGTGTT TGCTGAATGT 
CTTAGCGATA GCGCCATCTT TGTTCAGAGC CCGAACTGTA ATCAGAGGTA TGGCTGGCAT 
CCTGCAACAG TCTGTAAAAT CCCCCCAGGC TGTAACCTGA AGATCTTCAA CAACCAGGAG 
TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC CAGGGGTTCG AGGCTGTTTA TCAGTTGACC 
AGGATGTGCA CCATTCGCAT GAGTTTTGTC AAAGGCTGGG GAGCAGAGTA CAGACGGCAG 
ACAGTGACGA GCACCCCCTG CTGGATCGAG CTGCATCTGA ACGGGCCCCT CCAGTGGCTG 
GACAAGGTTC TGACCCAGAT GGGCTCTCCC TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334782.1
CDS	435..1751
Translation	MSSILPFTPPVVKRLLGWKKSASGSSGAGGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNRNTKCVTIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIETCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTNSIPENTNFPTGIEPPNNYIPETPPPGYISEDGEASDQQMNQSMDTGSPAELSPSTLSPVNHGMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Target ORF information:

RefSeq Version	XM_021479107.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 2 (smad2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479107.1

ORF Insert Sequence:

ATGTCCTCCA TCTTGCCTTT CACTCCGCCC GTGGTGAAAC GTCTGCTGGG TTGGAAGAAG 
TCTGCTAGTG GTTCAAGCGG AGCAGGAGGT GGTGGAGAGC AAAACGGCCA GGAGGAGAAG 
TGGTGCGAGA AGGCAGTCAA AAGCCTGGTA AAGAAGTTGA AGAAAACTGG CCAACTGGAC 
GAGCTGGAGA AGGCCATCAC CACACAAAAC CGCAACACCA AGTGTGTCAC CATTCCCAGA 
TCTCTGGACG GGCGTTTGCA AGTGTCTCAC CGTAAAGGTC TGCCTCATGT CATCTACTGC 
CGCCTGTGGC GATGGCCCGA CCTGCACAGC CATCACGAGC TGCGCGCCAT CGAGACCTGC 
GAGTATGCTT TCAACCTCAA GAAGGATGAA GTCTGCGTCA ATCCCTACCA CTACCAGCGG 
GTGGAGACAC CAGTTCTTCC TCCTGTTCTC GTGCCAAGAC ACACGGAGAT CCTGACTGAG 
TTGCCACCTT TGGACGACTA CACCAACTCC ATACCTGAAA ACACCAACTT CCCAACAGGG 
ATCGAGCCCC CCAACAATTA CATACCAGAA ACTCCTCCAC CGGGATACAT CAGTGAGGAT 
GGGGAGGCCA GTGACCAGCA AATGAATCAA AGTATGGACA CAGGTTCTCC TGCAGAACTG 
TCACCAAGCA CACTCTCGCC TGTCAATCAT GGCATGGACC TGCAGCCAGT GACTTACTCA 
GAGCCTGCGT TTTGGTGCTC CATAGCTTAC TATGAACTTA ACCAGCGGGT TGGAGAAACA 
TTCCACGCCT CTCAGCCTTC CCTCACCGTG GACGGCTTCA CAGACCCCTC CAATTCAGAG 
CGATTCTGCC TGGGCTTGCT GTCCAACGTC AACCGCAACG CCACCGTCGA GATGACCCGA 
AGACATATAG GACGAGGAGT CAGGCTGTAT TATATTGGTG GGGAAGTGTT TGCTGAATGT 
CTTAGCGATA GCGCCATCTT TGTTCAGAGC CCGAACTGTA ATCAGAGGTA TGGCTGGCAT 
CCTGCAACAG TCTGTAAAAT CCCCCCAGGC TGTAACCTGA AGATCTTCAA CAACCAGGAG 
TTTGCAGCAT TGCTGGCTCA GTCGGTGAAC CAGGGGTTCG AGGCTGTTTA TCAGTTGACC 
AGGATGTGCA CCATTCGCAT GAGTTTTGTC AAAGGCTGGG GAGCAGAGTA CAGACGGCAG 
ACAGTGACGA GCACCCCCTG CTGGATCGAG CTGCATCTGA ACGGGCCCCT CCAGTGGCTG 
GACAAGGTTC TGACCCAGAT GGGCTCTCCC TCCGTACGCT GCTCCAGTAT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification.
Development (Cambridge, England)144(21)4015-4025(2017 11)
Hozumi S,Aoki S,Kikuchi Y

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smad2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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