six3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	six3a
Entrez Gene ID	30635
Full Name	SIX homeobox 3a
Synonyms	six3,six3.1,wu:fc10a08
General protein information	Preferred Names SIX homeobox 3a Names homeobox protein SIX3 etID309945.13 etID46760.13 sine oculis homeobox homolog 3a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131362.1	NP_571437.1	homeobox protein SIX3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	six3	XP_002944388.2
Canis lupus familiaris (dog)	SIX3	XP_538477.4
Mus musculus (house mouse)	Six3	NP_035511.2
Rattus norvegicus (Norway rat)	Six3	NP_076480.1
Danio rerio (zebrafish)	six3a	NP_571437.1
Pan troglodytes (chimpanzee)	SIX3	XP_525749.4
Bos taurus (cattle)	SIX3	NP_001180053.1
Gallus gallus (chicken)	SIX3	NP_989695.1
Homo sapiens (human)	SIX3	NP_005404.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Genetic Dissection of Dual Roles for the Transcription Factor six7 in Photoreceptor Development and Patterning in Zebrafish.
Sotolongo-Lopez M, Alvarez-Delfin K, Saade CJ, Vera DL, Fadool JM
PLoS genetics12(4)e1005968(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings reveal new roles for Six3 in eye development
Title: Six3 regulates optic nerve development via multiple mechanisms.

data find that Six3 promotes ventral telencephalic fates through transient regulation of foxg1a expression and repression of the Wnt/beta-catenin pathway.
Title: Six3 cooperates with Hedgehog signaling to specify ventral telencephalon by promoting early expression of Foxg1a and repressing Wnt signaling.

the TAATGTC motif is an important target sequence for vertebrate Six3 proteins in vivo
Title: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins.

Study provides a comprehensive view of six3a transcription regulation during brain and eye development and offers an opportunity to establish the gene regulatory networks underlying neurogenesis in zebrafish.
Title: Complexity of cis-regulatory organization of six3a during forebrain and eye development in zebrafish.

These results identify Six3 as a direct regulator of Shh expression and reveal a crossregulatory loop between Shh and Six3 in the ventral forebrain.
Title: Haploinsufficiency of Six3 fails to activate Sonic hedgehog expression in the ventral forebrain and causes holoprosencephaly.

The following six3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the six3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03476	NM_131362.1 Latest version!	Danio rerio SIX homeobox 3a (six3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

six3a ( NM_131362.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571437.1 Danio rerio SIX homeobox 3a (six3a), mRNA.

Price & Availability↑

CloneID	ODa03476
Clone ID Related Accession (Same CDS sequence)	NM_131362.1
Accession Version	NM_131362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	homeobox protein SIX3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF030280.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF030280.1, BC059414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTTTTCA GATCGCCTTT AGAGCTTTAT CCCTCTCATT TCTTCCTGCC AAACTTTGCT 
GATCGCCCTT TGCTCTTGGC GAGCAGTGCG CCCAGCACCA GGTCTCCCGA AGACTTGTCA 
ATGTTTCAGC TACCGACCCT AAACTTCTCT CCAGAGCAAG TGGCGAGCGT CTGCGAGACG 
CTGGAGGAGA CCGGGGACAT CGAGCGGCTG GGTCGCTTCC TATGGTCGCT TCCAGTGGCA 
CCGGGTGCTT GCGAGGCGAT CAACAAGCAT GAATCCATCC TACGCGCCCG GGCTGTTGTC 
GCCTTTCACA CCGGCAATTT TCGCGATCTC TACCACATTT TGGAGAACCA CAAGTTTACC 
AAGGACTCGC ATGGTAAACT ACAAGCGATG TGGCTAGAGG CTCACTATCA GGAGGCCGAG 
AAACTACGCG GGCGTCCCCT AGGACCGGTT GATAAGTACA GGGTGCGAAA GAAGTTTCCC 
CTGCCTAGAA CCATCTGGGA CGGTGAGCAG AAGACGCATT GTTTCAAGGA GCGAACGCGG 
AGTCTGTTAC GAGAGTGGTA CCTTCAGGAC CCCTACCCAA ACCCCAGCAA GAAAAGGGAA 
CTGGCTCAAG CCACTGGACT CACTCCTACA CAGGTCGGAA ATTGGTTTAA AAACAGGAGA 
CAACGAGACA GGGCAGCGGC AGCAAAAAAC AGGCTCCAGC ATCAAGCAAT AGGGCAGAAT 
GGCATGCGGT CCCTTTCAGA ATCCGGCTGC GCCCCACGGA GTTCGGCGGA GTCGCCATCA 
ACTGCGGCTA GTCCAACAAC CAGTGTCTCG AGTATGACAG AACGAGTCGA CACGGGCACG 
TCAATTCTTT CAGTAACATC AAGTGACTCG GAATGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571437.1
CDS	117..1001
Translation	MVFRSPLELYPSHFFLPNFADRPLLLASSAPSTRSPEDLSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAFHTGNFRDLYHILENHKFTKDSHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGQNGMRSLSESGCAPRSSAESPSTAASPTTSVSSMTERVDTGTSILSVTSSDSECDV

Target ORF information:

RefSeq Version	NM_131362.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SIX homeobox 3a (six3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131362.1

ORF Insert Sequence:

ATGGTTTTCA GATCGCCTTT AGAGCTTTAT CCCTCTCATT TCTTCCTGCC AAACTTTGCT 
GATCGCCCTT TGCTCTTGGC GAGCAGTGCG CCCAGCACCA GGTCTCCCGA AGACTTGTCA 
ATGTTTCAGC TACCGACCCT AAACTTCTCT CCAGAGCAAG TGGCGAGCGT CTGCGAGACG 
CTGGAGGAGA CCGGGGACAT CGAGCGGCTG GGTCGCTTCC TATGGTCGCT TCCAGTGGCA 
CCGGGTGCTT GCGAGGCGAT CAACAAGCAT GAATCCATCC TACGCGCCCG GGCTGTTGTC 
GCCTTTCACA CCGGCAATTT TCGCGATCTC TACCACATTT TGGAGAACCA CAAGTTTACC 
AAGGACTCGC ATGGTAAACT ACAAGCGATG TGGCTAGAGG CTCACTATCA GGAGGCCGAG 
AAACTACGCG GGCGTCCCCT AGGACCGGTT GATAAGTACA GGGTGCGAAA GAAGTTTCCC 
CTGCCTAGAA CCATCTGGGA CGGTGAGCAG AAGACGCATT GTTTCAAGGA GCGAACGCGG 
AGTCTGTTAC GAGAGTGGTA CCTTCAGGAC CCCTACCCAA ACCCCAGCAA GAAAAGGGAA 
CTGGCTCAAG CCACTGGACT CACTCCTACA CAGGTCGGAA ATTGGTTTAA AAACAGGAGA 
CAACGAGACA GGGCAGCGGC AGCAAAAAAC AGGCTCCAGC ATCAAGCAAT AGGGCAGAAT 
GGCATGCGGT CCCTTTCAGA ATCCGGCTGC GCCCCACGGA GTTCGGCGGA GTCGCCATCA 
ACTGCGGCTA GTCCAACAAC CAGTGTCTCG AGTATGACAG AACGAGTCGA CACGGGCACG 
TCAATTCTTT CAGTAACATC AAGTGACTCG GAATGCGACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Genetic Dissection of Dual Roles for the Transcription Factor six7 in Photoreceptor Development and Patterning in Zebrafish.
PLoS genetics12(4)e1005968(2016 Apr)
Sotolongo-Lopez M,Alvarez-Delfin K,Saade CJ,Vera DL,Fadool JM