anos1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	anos1a
Entrez Gene ID	30630
Full Name	anosmin 1a
Synonyms	anosmin-1a,kal1.1,kal1a,wu:fl91z45
General protein information	Preferred Names anosmin 1a Names anosmin-1 Kallmann syndrome 1.1 sequence Kallmann syndrome 1a sequence
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131358.1	NP_571433.1	anosmin-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	kal1a	NP_571433.1
Pan troglodytes (chimpanzee)	KAL1	XP_520920.3
Canis lupus familiaris (dog)	KAL1	XP_853595.3
Gallus gallus (chicken)	KAL1	NP_990755.1
Caenorhabditis elegans (roundworm)	kal-1	NP_493468.2
Homo sapiens (human)	KAL1	NP_000207.2
	KAL1	XP_002806176.1
Bos taurus (cattle)	LOC525898	XP_005228592.1
Xenopus tropicalis (tropical clawed frog)	kal1	NP_001107160.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Point mutations in KAL1 and the mitochondrial gene MT-tRNA(cys) synergize to produce Kallmann syndrome phenotype.
Wang F, Huang GD, Tian H, Zhong YB, Shi HJ, Li Z, Zhang XS, Wang H, Sun F
Scientific reports513050(2015 Aug)

Functional development of the olfactory system in zebrafish.
Miyasaka N, Wanner AA, Li J, Mack-Bucher J, Genoud C, Yoshihara Y, Friedrich RW
Mechanisms of development130(6-8)336-46(2013 Jun-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data provide a consolidated map of expression of kal 1.a and kal 1.b
Title: Localization and characterization of kal 1.a and kal 1.b in the brain of adult zebrafish (Danio rerio).

Studies using wild-type and omp::GFP trangenic embryos reveal that anosmin-1a is required for differentiation of olfactory bulb neuron subpopulations
Title: Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system.

essential requirement for anosmin-1a in posterior lateral line primordium (PLLP) migration, and a key role for proper activation of the CXCR4b/SDF1a and/or CXCR7/SDF1a signalling pathway in PLLP migration
Title: Essential requirement for zebrafish anosmin-1a in the migration of the posterior lateral line primordium.

anosmin-1a encoded by kal1a and anosmin-1b,encoded by kal1b are expressed in sensory hair cells of the inner ear cristae ampullaris and the lateral line neuromasts
Title: Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish.

The following anos1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the anos1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34438	NM_131358.1 Latest version!	Danio rerio anosmin 1a (anos1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

anos1a ( NM_131358.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571433.1 Danio rerio anosmin 1a (anos1a), mRNA.

Price & Availability↑

CloneID	ODa34438
Clone ID Related Accession (Same CDS sequence)	NM_131358.1
Accession Version	NM_131358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Danio rerio(zebrafish)
Product	anosmin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF163310.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF163310.1, BC060938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCGCGACG GGCTCACCGG TTTGTGCATT GGGATTATTC TGTTATTAAA CGGCGGGCTG 
GTGGTTATGA GGAGACAGGA CTCGTCTTCA GCTCTTGGCA GTATTTTCCG GACGAGGTGC 
GCCGCCAGGT GCCTGAGCCT CCACAGCACG CGCATCGCTC TCTCGCCAAG ACATTTCCAG 
AGTAATGGAT CCCTTGGATG GTGTCAGAGC CATAAACAGT GTGCAAAGTG CCTGGAGCCA 
TGCAAAGACT CCTGGGAGTT GAAAGACGGA CCTTGTCGAG ATTTATGTGA GCATGCGTTT 
CCCAAGCGTC ATGGGGAGTG TGTGACGAGC TGTGAGTTCC TGCGATCGGT GATGGTGGTG 
AAGCAGGGTG ACTGTCCGCC ACCGGAGAGA GCAAGTGGTT TTGCAGCCGC CTGTGTGGAA 
GGATGTGAGG AAGATGGAGA GTGTTCAGGA CAGAAGAAAT GCTGTCCAAA TGGCTGCGGA 
CACACATGCC AGTCCCCTAA AAACCTCTAC AGAGGTGCTC CTCTGAAGCC CAGGAAGGAG 
CTGGTGTTTG AAGAGCTGGA GTCTGGTGTG TTGGAAGTGC GCTGGTCCTC CAAATTCAAT 
GTGTCGGCAG AGCCAGTTCT GAATGTTCTG CAGAGAAGGT GGAATTACGG CATCCACCCC 
AGTGAAGATG GAGCCACTGA GTGGCAAGTG GTGGCACGGA CATCAGGAGA ACGTGTGTGG 
CTGACAGACA TACGACCTGG TCGCTGGTAC CAGTTCAGAG TGGCAGCGGT CAATGTTCAT 
GGAACCAGAG GATACACCAC CCCTAGCAGA CACTTCAGAT CATCTAAAGA TCCTCCCCCT 
CCCCCGGCGC CATCTGATCT TCATGTTAGC GACATGACCT TTGGTGCGGA TCACTCGGCG 
TCTGTCCGGC TCAGCTGGAG CATTTCTGCA GATTTAGACA TCCCAGTAAA CCACTACAAG 
CTCTCCTGGA GCTCCTCTGA CCACAATCTG CCACCCAAAC TCAAGAAGAG AGAGACTGTA 
AACGGGGACT CTACCTATGC TGAGCTGGAT GATTTAAGAG AGAACGGGAG TTATACTGTG 
GAGCTCCAGG CAGTTTCATA CTGGGGTCAG GTCCCGCTCA AAAGCTCCAA GGCCATTCTT 
CAGTTTACTA CAAGCCAAAC ACAATCTGAT TTGGAGCCCA CAGTCAGTCC GGTCTTTATA 
AAGCCCCAGT CAGATCTACT AGACGTGGGC ACTCCATTCT ATCAAGATGG ACAGCTTCAG 
GTTCGGGTTT ATTGGAAAAA GAAAGATCCC ACCGTGAATC GTTACCGTGT GCAGTGGGCT 
CCAGAATTCT GCAGTCACAA CGGATCCAGA ACCCAGGGAA AGCTCATCAC CCAGGAAAAT 
TTTGCCAGCC TTCCCGGTCT TCAGTTTTCG TGCAAGTATA AAGTGATCAT CCAGCCTGTG 
GGGTTAAAGG GCCGAACCCA AGTGGAAAGC ACCACTTTCT ACACCCCGTC TTGTGCTACT 
ATCCAGAGCA AGAGTCCCAA ACCAATCCCA TGCTCCTCTG TCCCAGTGGT TCAGCCAAAG 
GTCCTGGTCA AGGCAGAAAA CCTGACAGCA GCATTTTCAG TCTACTTGGG GAACGTGACA 
GGCCTGTTCT CATGGGTAGT GGCCATGCCT CAGCAGCCAC AGCAGGTCAC AGGGTATCAG 
GTCACATGGG CAGAGGTCAT CACGGAAAGC CGGAGGAATA ATCTGCCCAA CAGCCTCATA 
TCCCAGTCTC AAATTCTGCC ACCAGAGCGC AACATCCTGG TAGTTTCTGG CCTTCAGTTA 
GCATCGCTCT ACAGACTTGA GGTGCAGGTG ATCACTGCAG CAGGACAAGG ACCAGCAACA 
TCCAGAACCT TTCAGACACC CACTCACAGC AAGCCTGTCC TGCATTACAA ACCCAGGATT 
AAGAAACACC ATCTAAGGTC AGTGATTGAA CGTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571433.1
CDS	48..2006
Translation	MRDGLTGLCIGIILLLNGGLVVMRRQDSSSALGSIFRTRCAARCLSLHSTRIALSPRHFQSNGSLGWCQSHKQCAKCLEPCKDSWELKDGPCRDLCEHAFPKRHGECVTSCEFLRSVMVVKQGDCPPPERASGFAAACVEGCEEDGECSGQKKCCPNGCGHTCQSPKNLYRGAPLKPRKELVFEELESGVLEVRWSSKFNVSAEPVLNVLQRRWNYGIHPSEDGATEWQVVARTSGERVWLTDIRPGRWYQFRVAAVNVHGTRGYTTPSRHFRSSKDPPPPPAPSDLHVSDMTFGADHSASVRLSWSISADLDIPVNHYKLSWSSSDHNLPPKLKKRETVNGDSTYAELDDLRENGSYTVELQAVSYWGQVPLKSSKAILQFTTSQTQSDLEPTVSPVFIKPQSDLLDVGTPFYQDGQLQVRVYWKKKDPTVNRYRVQWAPEFCSHNGSRTQGKLITQENFASLPGLQFSCKYKVIIQPVGLKGRTQVESTTFYTPSCATIQSKSPKPIPCSSVPVVQPKVLVKAENLTAAFSVYLGNVTGLFSWVVAMPQQPQQVTGYQVTWAEVITESRRNNLPNSLISQSQILPPERNILVVSGLQLASLYRLEVQVITAAGQGPATSRTFQTPTHSKPVLHYKPRIKKHHLRSVIERH

Target ORF information:

RefSeq Version	NM_131358.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anosmin 1a (anos1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131358.1

ORF Insert Sequence:

ATGCGCGACG GGCTCACCGG TTTGTGCATT GGGATTATTC TGTTATTAAA CGGCGGGCTG 
GTGGTTATGA GGAGACAGGA CTCGTCTTCA GCTCTTGGCA GTATTTTCCG GACGAGGTGC 
GCCGCCAGGT GCCTGAGCCT CCACAGCACG CGCATCGCTC TCTCGCCAAG ACATTTCCAG 
AGTAATGGAT CCCTTGGATG GTGTCAGAGC CATAAACAGT GTGCAAAGTG CCTGGAGCCA 
TGCAAAGACT CCTGGGAGTT GAAAGACGGA CCTTGTCGAG ATTTATGTGA GCATGCGTTT 
CCCAAGCGTC ATGGGGAGTG TGTGACGAGC TGTGAGTTCC TGCGATCGGT GATGGTGGTG 
AAGCAGGGTG ACTGTCCGCC ACCGGAGAGA GCAAGTGGTT TTGCAGCCGC CTGTGTGGAA 
GGATGTGAGG AAGATGGAGA GTGTTCAGGA CAGAAGAAAT GCTGTCCAAA TGGCTGCGGA 
CACACATGCC AGTCCCCTAA AAACCTCTAC AGAGGTGCTC CTCTGAAGCC CAGGAAGGAG 
CTGGTGTTTG AAGAGCTGGA GTCTGGTGTG TTGGAAGTGC GCTGGTCCTC CAAATTCAAT 
GTGTCGGCAG AGCCAGTTCT GAATGTTCTG CAGAGAAGGT GGAATTACGG CATCCACCCC 
AGTGAAGATG GAGCCACTGA GTGGCAAGTG GTGGCACGGA CATCAGGAGA ACGTGTGTGG 
CTGACAGACA TACGACCTGG TCGCTGGTAC CAGTTCAGAG TGGCAGCGGT CAATGTTCAT 
GGAACCAGAG GATACACCAC CCCTAGCAGA CACTTCAGAT CATCTAAAGA TCCTCCCCCT 
CCCCCGGCGC CATCTGATCT TCATGTTAGC GACATGACCT TTGGTGCGGA TCACTCGGCG 
TCTGTCCGGC TCAGCTGGAG CATTTCTGCA GATTTAGACA TCCCAGTAAA CCACTACAAG 
CTCTCCTGGA GCTCCTCTGA CCACAATCTG CCACCCAAAC TCAAGAAGAG AGAGACTGTA 
AACGGGGACT CTACCTATGC TGAGCTGGAT GATTTAAGAG AGAACGGGAG TTATACTGTG 
GAGCTCCAGG CAGTTTCATA CTGGGGTCAG GTCCCGCTCA AAAGCTCCAA GGCCATTCTT 
CAGTTTACTA CAAGCCAAAC ACAATCTGAT TTGGAGCCCA CAGTCAGTCC GGTCTTTATA 
AAGCCCCAGT CAGATCTACT AGACGTGGGC ACTCCATTCT ATCAAGATGG ACAGCTTCAG 
GTTCGGGTTT ATTGGAAAAA GAAAGATCCC ACCGTGAATC GTTACCGTGT GCAGTGGGCT 
CCAGAATTCT GCAGTCACAA CGGATCCAGA ACCCAGGGAA AGCTCATCAC CCAGGAAAAT 
TTTGCCAGCC TTCCCGGTCT TCAGTTTTCG TGCAAGTATA AAGTGATCAT CCAGCCTGTG 
GGGTTAAAGG GCCGAACCCA AGTGGAAAGC ACCACTTTCT ACACCCCGTC TTGTGCTACT 
ATCCAGAGCA AGAGTCCCAA ACCAATCCCA TGCTCCTCTG TCCCAGTGGT TCAGCCAAAG 
GTCCTGGTCA AGGCAGAAAA CCTGACAGCA GCATTTTCAG TCTACTTGGG GAACGTGACA 
GGCCTGTTCT CATGGGTAGT GGCCATGCCT CAGCAGCCAC AGCAGGTCAC AGGGTATCAG 
GTCACATGGG CAGAGGTCAT CACGGAAAGC CGGAGGAATA ATCTGCCCAA CAGCCTCATA 
TCCCAGTCTC AAATTCTGCC ACCAGAGCGC AACATCCTGG TAGTTTCTGG CCTTCAGTTA 
GCATCGCTCT ACAGACTTGA GGTGCAGGTG ATCACTGCAG CAGGACAAGG ACCAGCAACA 
TCCAGAACCT TTCAGACACC CACTCACAGC AAGCCTGTCC TGCATTACAA ACCCAGGATT 
AAGAAACACC ATCTAAGGTC AGTGATTGAA CGTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Point mutations in KAL1 and the mitochondrial gene MT-tRNA(cys) synergize to produce Kallmann syndrome phenotype.
Scientific reports513050(2015 Aug)
Wang F,Huang GD,Tian H,Zhong YB,Shi HJ,Li Z,Zhang XS,Wang H,Sun F

Functional development of the olfactory system in zebrafish.
Mechanisms of development130(6-8)336-46(2013 Jun-Aug)
Miyasaka N,Wanner AA,Li J,Mack-Bucher J,Genoud C,Yoshihara Y,Friedrich RW

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anos1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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