UBE2IB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2IB
Entrez Gene ID	30622
Full Name	ubiquitin-conjugating enzyme E2Ib
Synonyms	ubc9,ube2i,ube2ia,zUbc9
General protein information	Preferred Names ubiquitin-conjugating enzyme E2Ib Names SUMO-conjugating enzyme UBC9-A RING-type E3 SUMO transferase UBC9-A SUMO-protein ligase A ubc9.1 ubiquitin carrier protein 9-A ubiquitin carrier protein I-A ubiquitin-conjugating enzyme 9 ubiquitin-conjugating enzyme E2 I-A ubiquitin-conjugating enzyme E2I ubiquitin-protein ligase I-A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131351.2	NP_571426.1	SUMO-conjugating enzyme UBC9-A
XM_005174194.4	XP_005174251.1	SUMO-conjugating enzyme UBC9-A isoform X1
XM_005174194.3	XP_005174251.1	SUMO-conjugating enzyme UBC9-A isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ubc-9	NP_001023158.1
Danio rerio (zebrafish)	ube2i	NP_571426.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012107	XP_320422.3

Gene Ontology
Bibliography

Related articles in PubMed

Integrated in silico and in vivo approaches to investigate effects of BDE-99 mediated by the nuclear receptors on developing zebrafish.
Zhang L, Jin Y, Han Z, Liu H, Shi L, Hua X, Doering JA, Tang S, Giesy JP, Yu H
Environmental toxicology and chemistry(2017 Oct)

GATA5 SUMOylation is indispensable for zebrafish cardiac development.
Wen B, Yuan H, Liu X, Wang H, Chen S, Chen Z, de The H, Zhou J, Zhu J
Biochimica et biophysica acta1861(7)1691-1701(2017 07)

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation.
Pagano C, di Martino O, Ruggiero G, Maria Guarino A, Mueller N, Siauciunaite R, Reischl M, Simon Foulkes N, Vallone D, Calabr? V
Oncotarget8(4)6193-6205(2017 Jan)

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Yu F, Wang H, Liu W, Lu L
Fish & shellfish immunology48169-74(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate an in vivo requirement of Ubc9 for G2/M transition and/or progression through mitosis during vertebrate organogenesis.
Title: Ubc9 regulates mitosis and cell survival during zebrafish development.

The following UBE2IB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2IB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01072	NM_131351.2 Latest version!	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa28841	XM_005174194.4 Latest version!	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa28841	XM_005174194.3	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2IB ( NM_131351.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571426.1 Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), mRNA.

Price & Availability↑

CloneID	ODa01072
Clone ID Related Accession (Same CDS sequence)	NM_131351.2
Accession Version	NM_131351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Danio rerio(zebrafish)
Product	SUMO-conjugating enzyme UBC9-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059506.1. On May 16, 2004 this sequence version replaced NM_131351.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EL651154.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTCCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571426.1
CDS	129..605
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPMKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSPS

Target ORF information:

RefSeq Version	NM_131351.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131351.2

ORF Insert Sequence:

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTCCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2IB ( XM_005174194.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174251.1 Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28841
Clone ID Related Accession (Same CDS sequence)	XM_005174194.4 , XM_005174194.3
Accession Version	XM_005174194.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SUMO-conjugating enzyme UBC9-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174194.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTTCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174251.1
CDS	162..638
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPMKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSPS

Target ORF information:

RefSeq Version	XM_005174194.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174194.4

ORF Insert Sequence:

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTTCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2IB ( XM_005174194.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174251.1 Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28841
Clone ID Related Accession (Same CDS sequence)	XM_005174194.4 , XM_005174194.3
Accession Version	XM_005174194.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SUMO-conjugating enzyme UBC9-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTTCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174251.1
CDS	156..632
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPMKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSPS

Target ORF information:

RefSeq Version	XM_005174194.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-conjugating enzyme E2Ib (ube2ib), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174194.3

ORF Insert Sequence:

ATGTCTGGCA TTGCTCTGAG TCGACTTGCA CAGGAGCGCA AAGCATGGAG GAAAGACCAT 
CCATTCGGCT TTGTTGCTGT GCCGATGAAA AACCCCGATG GTACCATGAA CCTTATGAAC 
TGGGAATGTG CTATTCCAGG AAAGAAGGGG ACTCCATGGG AAGGAGGTCT GTTTAAGTTA 
CGGATGCTGT TTAAAGACGA TTACCCTTCA TCACCCCCAA AGTGCAAATT TGAGCCTCCG 
CTGTTTCATC CGAATGTGTA TCCGTCAGGA ACAGTGTGTC TTTCCATCCT GGAGGAGGAT 
AAAGACTGGA GACCCGCCAT CACAATTAAA CAGATCCTGT TGGGGATTCA GGAGCTTCTA 
AATGAACCGA ACATCCAGGA TCCAGCTCAG GCAGAGGCGT ACACCATATA CTGTCAGAAT 
AGAGTGGAGT ACGAGAAGAG AGTTCGTGCA CAGGCCAAAA AGTTCTCCCC GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Integrated in silico and in vivo approaches to investigate effects of BDE-99 mediated by the nuclear receptors on developing zebrafish.
Environmental toxicology and chemistry(2017 Oct)
Zhang L,Jin Y,Han Z,Liu H,Shi L,Hua X,Doering JA,Tang S,Giesy JP,Yu H

GATA5 SUMOylation is indispensable for zebrafish cardiac development.
Biochimica et biophysica acta1861(7)1691-1701(2017 07)
Wen B,Yuan H,Liu X,Wang H,Chen S,Chen Z,de The H,Zhou J,Zhu J

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation.
Oncotarget8(4)6193-6205(2017 Jan)
Pagano C,di Martino O,Ruggiero G,Maria Guarino A,Mueller N,Siauciunaite R,Reischl M,Simon Foulkes N,Vallone D,Calabr? V

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Fish & shellfish immunology48169-74(2016 Jan)
Yu F,Wang H,Liu W,Lu L

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UBE2IB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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