otx1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	otx1
Entrez Gene ID	30500
Full Name	orthodenticle homeobox 1
Synonyms	cb769,fj99f05,otx1b,wu:fj99f05,zOtx1
General protein information	Preferred Names orthodenticle homeobox 1 Names homeobox protein OTX1 B orthodenticle homeobox 1b orthodenticle homolog 1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131250.2	NP_571325.2	homeobox protein OTX1 B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Otx1	NP_035153.1
Homo sapiens (human)	OTX1	NP_055377.1
Xenopus tropicalis (tropical clawed frog)	otx1	NP_989216.1
	LOC694175	XP_001084150.1
Canis lupus familiaris (dog)	OTX1	XP_852530.1
Pan troglodytes (chimpanzee)	OTX1	XP_001162799.1
Bos taurus (cattle)	OTX1	NP_001192946.1
Rattus norvegicus (Norway rat)	Otx1	NP_037241.1
Danio rerio (zebrafish)	otx1b	NP_571325.2

Gene Ontology
Bibliography

Related articles in PubMed

sox2 and sox3 play unique roles in development of hair cells and neurons in the zebrafish inner ear.
Gou Y, Vemaraju S, Sweet EM, Kwon HJ, Riley BB
Developmental biology(2018 Jan)

Expansion microscopy of zebrafish for neuroscience and developmental biology studies.
Freifeld L, Odstrcil I, F?rster D, Ramirez A, Gagnon JA, Randlett O, Costa EK, Asano S, Celiker OT, Gao R, Martin-Alarcon DA, Reginato P, Dick C, Chen L, Schoppik D, Engert F, Baier H, Boyden ES
Proceedings of the National Academy of Sciences of the United States of America114(50)E10799-E10808(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that one or more Otx targets in addition to mitfa and mitfb, possibly another mitf family member, are necessary for development of the retinal pigmented epithelium in zebrafish.
Title: Otx but not Mitf transcription factors are required for zebrafish retinal pigment epithelium development.

Foxa1 binds to the otx1b non-conserved enhancer to direct its activity in forebrain and otic vesicle of zebrafish at 24 hpf.
Title: Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genes.

Results suggest that for transcriptional activation of at least one gene in the cell aggregation pathway, zOtx1 need not bind directly to DNA, but does require helix 1 and 2 of its homeodomain to interact with an as yet undefined DNA binding protein.
Title: Molecular dissection of Otx1 functional domains in the zebrafish embryo.

otx1 expression can account for all major patterning differences between zebrafish and lampreys.
Title: The developing lamprey ear closely resembles the zebrafish otic vesicle: otx1 expression can account for all major patterning differences.

The following otx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03619	NM_131250.2 Latest version!	Danio rerio orthodenticle homeobox 1 (otx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

otx1 ( NM_131250.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571325.2 Danio rerio orthodenticle homeobox 1 (otx1), mRNA.

Price & Availability↑

CloneID	ODa03619
Clone ID Related Accession (Same CDS sequence)	NM_131250.2
Accession Version	NM_131250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	homeobox protein OTX1 B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045290.1. On Dec 20, 2003 this sequence version replaced NM_131250.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045290.1, D26172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGTCAT ACCTCAAACA GCCCCCCTAC GCCATGAACG GCCTGGGGCT GAGCGGCGCG 
GCCATGGACC TACTACACCC GTCCGTCGGA TACCCAGCAA CACCGAGAAA GCAGCGGCGG 
GAGAGGACTA CATTTACGCG CTCACAGCTT GACATTCTCG AAGCGCTCTT TGCCAAAACT 
CGTTATCCGG ACATATTTAT GAGAGAGGAG GTGGCACTGA AGATCAACCT TCCCGAGTCC 
CGAGTTCAGG TGTGGTTTAA GAATCGTCGT GCTAAATGTC GTCAGCAGCA GCAGAGTGGC 
AGCAGCACGA AAACTCGGCC TGCAAAGAAG AAATCCTCAC CCACCAGGGA GAGCACAGGC 
TCTGAGAGCA GCGGCCAGTT CACCCCTCCT GCAGTCTCCA GCGCTGGCTC CTCGTCCTCC 
TCCTCTTCCT CCACCAATAA CACAGGCATC TGTAGCACCT CTACCTCTAT CAGTACTGTC 
TCCTCAATCT GGAGCCCTGC CATTTCTCCA GGCTCAGCAC CCCCATCTGT GTCCCTGCCG 
GAGCCTGTCG CTCCCTCAAA CACCTCCTGC ATGCAGCGTT CCGTCTCAGG CACAGCCAGC 
TCCACCTACC CCATGCCCTA CAACCAGACC ACCGGCTACA GCCAGGGCTA CCCGACTCCG 
TCCGGTTCTT ACTTCAGTGG GGTGGACTGT GGGTCCTATC TGGCCCCCAT GCACTCTCAC 
CACCACCCTC ATCAGCTCAG CCCCATGACG GCCTCATCCA TGCCCACACA CCCCCATCAC 
CACATCAGCC AGTCCTCAGG ACACCACCAC CACCATCACC ACCAGGCCTA CAGTGGCACG 
GGGCTGGCCT TCAATTCCTC TGACTGCCTG GATTACAAGG AGCAAACGGC CTCCTCCTGG 
AAGCTCAACT TCAACACTAC AGACTGTTTG GACTACAAAG ACCAAGCGTC CTGGAGGTTC 
CAAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571325.2
CDS	426..1397
Translation	MMSYLKQPPYAMNGLGLSGAAMDLLHPSVGYPATPRKQRRERTTFTRSQLDILEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSSTKTRPAKKKSSPTRESTGSESSGQFTPPAVSSAGSSSSSSSSTNNTGICSTSTSISTVSSIWSPAISPGSAPPSVSLPEPVAPSNTSCMQRSVSGTASSTYPMPYNQTTGYSQGYPTPSGSYFSGVDCGSYLAPMHSHHHPHQLSPMTASSMPTHPHHHISQSSGHHHHHHHQAYSGTGLAFNSSDCLDYKEQTASSWKLNFNTTDCLDYKDQASWRFQVL

Target ORF information:

RefSeq Version	NM_131250.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio orthodenticle homeobox 1 (otx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131250.2

ORF Insert Sequence:

ATGATGTCAT ACCTCAAACA GCCCCCCTAC GCCATGAACG GCCTGGGGCT GAGCGGCGCG 
GCCATGGACC TACTACACCC GTCCGTCGGA TACCCAGCAA CACCGAGAAA GCAGCGGCGG 
GAGAGGACTA CATTTACGCG CTCACAGCTT GACATTCTCG AAGCGCTCTT TGCCAAAACT 
CGTTATCCGG ACATATTTAT GAGAGAGGAG GTGGCACTGA AGATCAACCT TCCCGAGTCC 
CGAGTTCAGG TGTGGTTTAA GAATCGTCGT GCTAAATGTC GTCAGCAGCA GCAGAGTGGC 
AGCAGCACGA AAACTCGGCC TGCAAAGAAG AAATCCTCAC CCACCAGGGA GAGCACAGGC 
TCTGAGAGCA GCGGCCAGTT CACCCCTCCT GCAGTCTCCA GCGCTGGCTC CTCGTCCTCC 
TCCTCTTCCT CCACCAATAA CACAGGCATC TGTAGCACCT CTACCTCTAT CAGTACTGTC 
TCCTCAATCT GGAGCCCTGC CATTTCTCCA GGCTCAGCAC CCCCATCTGT GTCCCTGCCG 
GAGCCTGTCG CTCCCTCAAA CACCTCCTGC ATGCAGCGTT CCGTCTCAGG CACAGCCAGC 
TCCACCTACC CCATGCCCTA CAACCAGACC ACCGGCTACA GCCAGGGCTA CCCGACTCCG 
TCCGGTTCTT ACTTCAGTGG GGTGGACTGT GGGTCCTATC TGGCCCCCAT GCACTCTCAC 
CACCACCCTC ATCAGCTCAG CCCCATGACG GCCTCATCCA TGCCCACACA CCCCCATCAC 
CACATCAGCC AGTCCTCAGG ACACCACCAC CACCATCACC ACCAGGCCTA CAGTGGCACG 
GGGCTGGCCT TCAATTCCTC TGACTGCCTG GATTACAAGG AGCAAACGGC CTCCTCCTGG 
AAGCTCAACT TCAACACTAC AGACTGTTTG GACTACAAAG ACCAAGCGTC CTGGAGGTTC 
CAAGTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

sox2 and sox3 play unique roles in development of hair cells and neurons in the zebrafish inner ear.
Developmental biology(2018 Jan)
Gou Y,Vemaraju S,Sweet EM,Kwon HJ,Riley BB

Expansion microscopy of zebrafish for neuroscience and developmental biology studies.
Proceedings of the National Academy of Sciences of the United States of America114(50)E10799-E10808(2017 Dec)
Freifeld L,Odstrcil I,F?rster D,Ramirez A,Gagnon JA,Randlett O,Costa EK,Asano S,Celiker OT,Gao R,Martin-Alarcon DA,Reginato P,Dick C,Chen L,Schoppik D,Engert F,Baier H,Boyden ES

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otx1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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