gRNAs
The following brd2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brd2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa150055 | |
| Clone ID Related Accession (Same CDS sequence) | XM_021467982.1 | |
| Accession Version | XM_021467982.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | bromodomain-containing protein 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_021323657.1 |
| CDS | 110..1549 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_021467982.1 |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa02968 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001270571.1 | |
| Accession Version | NM_001270571.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-04 | |
| Organism | Danio rerio(zebrafish) | |
| Product | bromodomain-containing protein 2 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB772248.1, BC163902.1 and FP085392.6. Transcript Variant: This variant (2) uses an alternate splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N-and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01002523.1, GDQQ01001399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001257500.1 |
| CDS | 109..2607 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001270571.1 |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa03336 | |
| Clone ID Related Accession (Same CDS sequence) | NM_131200.2 | |
| Accession Version | NM_131200.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-04 | |
| Organism | Danio rerio(zebrafish) | |
| Product | bromodomain-containing protein 2 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB772248.1, BC163902.1 and FP085392.6. On Jul 19, 2012 this sequence version replaced NM_131200.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU126946.1, EU126947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_571275.2 |
| CDS | 109..2619 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_131200.2 |
1 | ATGGAAACGG CTCTTAGCCC GCCCTTTGAC AGCTCCCTGG GCGGGTGCGA CGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish. |
Alternative haplotypes of antigen processing genes in zebrafish diverged early in vertebrate evolution. |
Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database. |
