smn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smn1
Entrez Gene ID	30432
Full Name	survival of motor neuron 1, telomeric
Synonyms	fa12d01,smn,wu:fa12d01
General protein information	Preferred Names survival of motor neuron 1, telomeric Names survival motor neuron protein 1 survival motor neuron 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131191.1	NP_571266.1	survival motor neuron protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SMN1	NP_783632.1
Rattus norvegicus (Norway rat)	Smn1	NP_071954.1
Gallus gallus (chicken)	SMN	NP_989530.1
Homo sapiens (human)	SMN2	NP_075014.1
Pan troglodytes (chimpanzee)	SMN1	XP_001156259.1
Mus musculus (house mouse)	Smn1	NP_035550.1
Homo sapiens (human)	SMN1	NP_075012.1
	SMN1	XP_001096348.2
Canis lupus familiaris (dog)	SMN	NP_001003226.1
Danio rerio (zebrafish)	smn1	NP_571266.1
Xenopus tropicalis (tropical clawed frog)	smn2	NP_001093710.1

Gene Ontology
Bibliography

Related articles in PubMed

HuD and the Survival Motor Neuron Protein Interact in Motoneurons and Are Essential for Motoneuron Development, Function, and mRNA Regulation.
Hao le T, Duy PQ, An M, Talbot J, Iyer CC, Wolman M, Beattie CE
The Journal of neuroscience : the official journal of the Society for Neuroscience37(48)11559-11571(2017 Nov)

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.
Boyd PJ, Tu WY, Shorrock HK, Groen EJN, Carter RN, Powis RA, Thomson SR, Thomson D, Graham LC, Motyl AAL, Wishart TM, Highley JR, Morton NM, Becker T, Becker CG, Heath PR, Gillingwater TH
PLoS genetics13(4)e1006744(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In motor neurons, smn1 interacts with the RNA binding protein, HuD and regulates motoneuron development and function.
Title: HuD and the Survival Motor Neuron Protein Interact in Motoneurons and Are Essential for Motoneuron Development, Function, and mRNA Regulation.

our finding that elevation of Pgk1 levels or activity, via injection of Pgk1 mRNA or treatment with terazosin respectively, robustly ameliorated MN pathology in smn morphant zebrafish provides an initial demonstration that these pathways are amenable to therapeutic intervention.
Title: Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

SMN deficiency affects splicing and abundance of nrxn2a. This may explain the pre-synaptic defects at neuromuscular endplates in SMA pathophysiology.
Title: SMN deficiency alters Nrxn2 expression and splicing in zebrafish and mouse models of spinal muscular atrophy.

SMN is needed early in development of motoneuron dendrites and axons to develop normally and that this is essential for proper connectivity and movement.
Title: Temporal requirement for SMN in motoneuron development.

Smn mutants in zebrafish survive to larval stages and exhibit presynaptic neuromuscular junction defects.
Title: Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects.

The following smn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02589	NM_131191.1 Latest version!	Danio rerio survival of motor neuron 1, telomeric (smn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smn1 ( NM_131191.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571266.1 Danio rerio survival of motor neuron 1, telomeric (smn1), mRNA.

Price & Availability↑

CloneID	ODa02589
Clone ID Related Accession (Same CDS sequence)	NM_131191.1
Accession Version	NM_131191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Danio rerio(zebrafish)
Product	survival motor neuron protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y17256.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y17256.1, EH543179.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAATG GTGCAGAAGA TGTCGTATTT TGTCGTGGGA CTGGTCAAAG TGATGATTCT 
GACATTTGGG ATGATACAGC TTTGATTAAA GCATACGATA AAGCTGTTGC ATCATTCAAG 
AATGCTTTGA AAGGTGAGGA TGGGGCGACC CCACAAGAAA ACGACAACCC AGGGAAGAAG 
AGGAAAAACA ACAAAAAGAA TAAGAGCAGG AAGAGATGCA ACGCAGCACC GGATAAAGAG 
TGGCAGGTTG GAGACTCCTG TTATGCGTTC TGGTCTGAAG ATGGCAACCT GTACACTGCC 
ACCATTACCT CAGTCGACCA GGAGAAGGGC ACCTGTGTGG TCTTTTACAC AGATTATGGA 
AATGAGGAGG AGCAGAACCT CAGTGACCTT CTGACTGAGC CTCCAGACAT GGATGAAGAT 
GCTCTGAAGA CAGCAAATGT CAAAGAAACA GAGTCCTCCA CAGAAGAGAG TGATCGCTCT 
TTCACCCCAC AGAAGTCCGG TCATGCAAAG CACAAATCTA AAAGCAATTT TCCTATGGGA 
CCCCCATCAT GGTTTCCCAG TTTCCCACCT GGACCGCCAC CACCACCACC CCACTTTAAA 
AAGATGGATG GCAGACGAGG AGAAGGTCCT GGTCCTTCCT TTCCTGGATG GCCTCCCATG 
ATTCCACTCG GTCCACCGAT GATCCCACCA CCGCCACCTA TGAGTCCAGA CTTTGGGGAG 
GACGACGAGG CTTTGGGCAG TATGCTGATT TCCTGGTATA TGAGCGGCTA TCACACTGGA 
TACTATATGG GTTTAAGACA GGGCCGTAAA GAGGCTGCTG CATCCAAGAA ATCGCATCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571266.1
CDS	72..917
Translation	MANGAEDVVFCRGTGQSDDSDIWDDTALIKAYDKAVASFKNALKGEDGATPQENDNPGKKRKNNKKNKSRKRCNAAPDKEWQVGDSCYAFWSEDGNLYTATITSVDQEKGTCVVFYTDYGNEEEQNLSDLLTEPPDMDEDALKTANVKETESSTEESDRSFTPQKSGHAKHKSKSNFPMGPPSWFPSFPPGPPPPPPHFKKMDGRRGEGPGPSFPGWPPMIPLGPPMIPPPPPMSPDFGEDDEALGSMLISWYMSGYHTGYYMGLRQGRKEAAASKKSHRK

Target ORF information:

RefSeq Version	NM_131191.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio survival of motor neuron 1, telomeric (smn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131191.1

ORF Insert Sequence:

ATGGCCAATG GTGCAGAAGA TGTCGTATTT TGTCGTGGGA CTGGTCAAAG TGATGATTCT 
GACATTTGGG ATGATACAGC TTTGATTAAA GCATACGATA AAGCTGTTGC ATCATTCAAG 
AATGCTTTGA AAGGTGAGGA TGGGGCGACC CCACAAGAAA ACGACAACCC AGGGAAGAAG 
AGGAAAAACA ACAAAAAGAA TAAGAGCAGG AAGAGATGCA ACGCAGCACC GGATAAAGAG 
TGGCAGGTTG GAGACTCCTG TTATGCGTTC TGGTCTGAAG ATGGCAACCT GTACACTGCC 
ACCATTACCT CAGTCGACCA GGAGAAGGGC ACCTGTGTGG TCTTTTACAC AGATTATGGA 
AATGAGGAGG AGCAGAACCT CAGTGACCTT CTGACTGAGC CTCCAGACAT GGATGAAGAT 
GCTCTGAAGA CAGCAAATGT CAAAGAAACA GAGTCCTCCA CAGAAGAGAG TGATCGCTCT 
TTCACCCCAC AGAAGTCCGG TCATGCAAAG CACAAATCTA AAAGCAATTT TCCTATGGGA 
CCCCCATCAT GGTTTCCCAG TTTCCCACCT GGACCGCCAC CACCACCACC CCACTTTAAA 
AAGATGGATG GCAGACGAGG AGAAGGTCCT GGTCCTTCCT TTCCTGGATG GCCTCCCATG 
ATTCCACTCG GTCCACCGAT GATCCCACCA CCGCCACCTA TGAGTCCAGA CTTTGGGGAG 
GACGACGAGG CTTTGGGCAG TATGCTGATT TCCTGGTATA TGAGCGGCTA TCACACTGGA 
TACTATATGG GTTTAAGACA GGGCCGTAAA GAGGCTGCTG CATCCAAGAA ATCGCATCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HuD and the Survival Motor Neuron Protein Interact in Motoneurons and Are Essential for Motoneuron Development, Function, and mRNA Regulation.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(48)11559-11571(2017 Nov)
Hao le T,Duy PQ,An M,Talbot J,Iyer CC,Wolman M,Beattie CE

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.
PLoS genetics13(4)e1006744(2017 Apr)
Boyd PJ,Tu WY,Shorrock HK,Groen EJN,Carter RN,Powis RA,Thomson SR,Thomson D,Graham LC,Motyl AAL,Wishart TM,Highley JR,Morton NM,Becker T,Becker CG,Heath PR,Gillingwater TH

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smn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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