srfa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	srfa
Entrez Gene ID	30431
Full Name	serum response factor a
Synonyms	srf,srf1
General protein information	Preferred Names serum response factor a Names serum response factor a DreSrf1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005174054.3	XP_005174111.1	serum response factor a isoform X2
NM_001110526.1	NP_001103996.1	serum response factor a
XM_017353104.1	XP_017208593.1	serum response factor a isoform X1
XM_005174054.4	XP_005174111.1	serum response factor a isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRF	NP_003122.1
Pan troglodytes (chimpanzee)	SRF	XP_518487.4
Bos taurus (cattle)	SRF	NP_001192945.1
Mus musculus (house mouse)	Srf	NP_065239.1
Danio rerio (zebrafish)	srfa	NP_001103996.1
Xenopus tropicalis (tropical clawed frog)	srf	XP_002942523.2
Gallus gallus (chicken)	SRF	NP_001239070.1
	SRF	XP_001093365.2
Canis lupus familiaris (dog)	SRF	XP_852302.1
Rattus norvegicus (Norway rat)	Srf	NP_001102772.1

Gene Ontology
Bibliography

Related articles in PubMed

Ferulic Acid Promotes Hypertrophic Growth of Fast Skeletal Muscle in Zebrafish Model.
Wen Y, Ushio H
Nutrients9(10)(2017 Sep)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Discovering miRNA Regulatory Networks in Holt-Oram Syndrome Using a Zebrafish Model.
D'Aurizio R, Russo F, Chiavacci E, Baumgart M, Groth M, D'Onofrio M, Arisi I, Rainaldi G, Pitto L, Pellegrini M
Frontiers in bioengineering and biotechnology460(2016)

MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome.
Chiavacci E, D'Aurizio R, Guzzolino E, Russo F, Baumgart M, Groth M, Mariani L, D'Onofrio M, Arisi I, Pellegrini M, Cellerino A, Cremisi F, Pitto L
Scientific reports518240(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel STARS-SRF feed-forward autoregulatory loop could play an essential role in STARS regulation and cardiac function
Title: STARS is essential to maintain cardiac development and function in vivo via a SRF pathway.

SRF was duplicated in the zebrafish genome and its protein expression in all three muscle cell types is highly conserved across vertebrate animals suggesting an ancient code for transcriptional regulation of genes unique to muscle cell lineages.
Title: Expression and comparative genomics of two serum response factor genes in zebrafish.

The following srfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00505	NM_001110526.1 Latest version!	Danio rerio serum response factor a (srfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa27190	XM_005174054.4 Latest version!	Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa57018	XM_017353104.1 Latest version!	Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa27190	XM_005174054.3	Danio rerio serum response factor a (srfa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

srfa ( NM_001110526.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103996.1 Danio rerio serum response factor a (srfa), mRNA.

Price & Availability↑

CloneID	ODa00505
Clone ID Related Accession (Same CDS sequence)	NM_001110526.1
Accession Version	NM_001110526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	serum response factor a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR925810.5. On or before Apr 10, 2008 this sequence version replaced NM_131190.1, XM_001333912.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01039655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA4476730 [ECO:0000348] ##Evidence-Data-END##

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGC GCTTCTCTTC CGCCGGGCAC ACACACTGTT 
CCTCTCAGTC AGCTGCAGAC ACACTCTCTG GCCATACAGT GTCCACAGAC GCAGGCCAAC 
AGCAGCGGCA GCACTGCTGT CCCCGCACAC ACACAACCCG CCGCGCTCTT CCGCCTGCCA 
GCCGCCGTCT CACTCACAGG TGGAGCGATG CCTCAACAGC TGCAAGCCAT CCAGGTTCAT 
CCCAGTCCTC AGAGCAGCGA CAGCAGTCCT GAGATCAGCC AGACGTCCAG CAGCGCCACT 
GCTAGCCACA CCGCGACTAT AGTCACGTCC TCAGTGCCTT CTTCAGTGGC CGGACACATG 
ATGTACCCTG GAGCTCACGT CATGTACGCC ACCTCCACCC CCGCCCTTAC TGACGGAGGT 
CTGACTGTGC TCAGCGCCTT CCCACAACCC CCCAGTGCAA TGCATGTGTC GCACACACCG 
AATCAGGACT CAGGTGGAGT TCCTCAGATG TTCCTCACAG GACCTCCTGG CACTGTACAG 
ATCCCAGTTT CAGCCGTTCC CCTTCATTCG ATGTTAATAA ACCAGCAGCC CTCTGGCAGC 
AGCAGTAGCA CCCTCACCGA GCTGCGCGTG GTCAATCTGG ACAATCCCAA AGGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103996.1
CDS	319..1938
Translation	MLGGNGTARGAGRSGNGTGLTPLQVGSTSGALGAVNSVINLPDGGRYDDGPSVEAVLSGSDGDSDSGDDEESPVPDRRGVKRERSELEVGGSSGSNAALSAGYGGVSSGVAGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRTDPSTDQRMSATGFEETDLTYQVSEADGLTEPKEMLKPTFTAATLPGGTNTISTTTQSPVSSSPSSSSVAMHTQSSGPSLSGTTCWPVAAGMNAQSISSANGTVLKSSSAGMMQLPSGFTLMPGASLPPGTHTVPLSQLQTHSLAIQCPQTQANSSGSTAVPAHTQPAALFRLPAAVSLTGGAMPQQLQAIQVHPSPQSSDSSPEISQTSSSATASHTATIVTSSVPSSVAGHMMYPGAHVMYATSTPALTDGGLTVLSAFPQPPSAMHVSHTPNQDSGGVPQMFLTGPPGTVQIPVSAVPLHSMLINQQPSGSSSSTLTELRVVNLDNPKGD

Target ORF information:

RefSeq Version	NM_001110526.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serum response factor a (srfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110526.1

ORF Insert Sequence:

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGC GCTTCTCTTC CGCCGGGCAC ACACACTGTT 
CCTCTCAGTC AGCTGCAGAC ACACTCTCTG GCCATACAGT GTCCACAGAC GCAGGCCAAC 
AGCAGCGGCA GCACTGCTGT CCCCGCACAC ACACAACCCG CCGCGCTCTT CCGCCTGCCA 
GCCGCCGTCT CACTCACAGG TGGAGCGATG CCTCAACAGC TGCAAGCCAT CCAGGTTCAT 
CCCAGTCCTC AGAGCAGCGA CAGCAGTCCT GAGATCAGCC AGACGTCCAG CAGCGCCACT 
GCTAGCCACA CCGCGACTAT AGTCACGTCC TCAGTGCCTT CTTCAGTGGC CGGACACATG 
ATGTACCCTG GAGCTCACGT CATGTACGCC ACCTCCACCC CCGCCCTTAC TGACGGAGGT 
CTGACTGTGC TCAGCGCCTT CCCACAACCC CCCAGTGCAA TGCATGTGTC GCACACACCG 
AATCAGGACT CAGGTGGAGT TCCTCAGATG TTCCTCACAG GACCTCCTGG CACTGTACAG 
ATCCCAGTTT CAGCCGTTCC CCTTCATTCG ATGTTAATAA ACCAGCAGCC CTCTGGCAGC 
AGCAGTAGCA CCCTCACCGA GCTGCGCGTG GTCAATCTGG ACAATCCCAA AGGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srfa ( XM_005174054.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174111.1 Danio rerio serum response factor a (srfa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27190
Clone ID Related Accession (Same CDS sequence)	XM_005174054.3 , XM_005174054.4
Accession Version	XM_005174054.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serum response factor a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGT GGAGCGATGC CTCAACAGCT GCAAGCCATC 
CAGGTTCATC CCAGTCCTCA GAGCAGCGAC AGCAGTCCTG AGATCAGCCA GACGTCCAGC 
AGCGCCACTG CTAGCCACAC CGCGACTATA GTCACGTCCT CAGTGCCTTC TTCAGTGGCC 
GGACACATGA TGTACCCTGG AGCTCACGTC ATGTACGCCA CCTCCACCCC CGCCCTTACT 
GACGGAGGTC TGACTGTGCT CAGCGCCTTC CCACAACCCC CCAGTGCAAT GCATGTGTCG 
CACACACCGA ATCAGGACTC AGGTGGAGTT CCTCAGATGT TCCTCACAGG ACCTCCTGGC 
ACTGTACAGA TCCCAGTTTC AGCCGTTCCC CTTCATTCGA TGTTAATAAA CCAGCAGCCC 
TCTGGCAGCA GCAGTAGCAC CCTCACCGAG CTGCGCGTGG TCAATCTGGA CAATCCCAAA 
GGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174111.1
CDS	381..1829
Translation	MLGGNGTARGAGRSGNGTGLTPLQVGSTSGALGAVNSVINLPDGGRYDDGPSVEAVLSGSDGDSDSGDDEESPVPDRRGVKRERSELEVGGSSGSNAALSAGYGGVSSGVAGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRTDPSTDQRMSATGFEETDLTYQVSEADGLTEPKEMLKPTFTAATLPGGTNTISTTTQSPVSSSPSSSSVAMHTQSSGPSLSGTTCWPVAAGMNAQSISSANGTVLKSSSAGMMQLPSGFTLMPGGAMPQQLQAIQVHPSPQSSDSSPEISQTSSSATASHTATIVTSSVPSSVAGHMMYPGAHVMYATSTPALTDGGLTVLSAFPQPPSAMHVSHTPNQDSGGVPQMFLTGPPGTVQIPVSAVPLHSMLINQQPSGSSSSTLTELRVVNLDNPKGD

Target ORF information:

RefSeq Version	XM_005174054.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio serum response factor a (srfa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174054.3

ORF Insert Sequence:

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGT GGAGCGATGC CTCAACAGCT GCAAGCCATC 
CAGGTTCATC CCAGTCCTCA GAGCAGCGAC AGCAGTCCTG AGATCAGCCA GACGTCCAGC 
AGCGCCACTG CTAGCCACAC CGCGACTATA GTCACGTCCT CAGTGCCTTC TTCAGTGGCC 
GGACACATGA TGTACCCTGG AGCTCACGTC ATGTACGCCA CCTCCACCCC CGCCCTTACT 
GACGGAGGTC TGACTGTGCT CAGCGCCTTC CCACAACCCC CCAGTGCAAT GCATGTGTCG 
CACACACCGA ATCAGGACTC AGGTGGAGTT CCTCAGATGT TCCTCACAGG ACCTCCTGGC 
ACTGTACAGA TCCCAGTTTC AGCCGTTCCC CTTCATTCGA TGTTAATAAA CCAGCAGCCC 
TCTGGCAGCA GCAGTAGCAC CCTCACCGAG CTGCGCGTGG TCAATCTGGA CAATCCCAAA 
GGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srfa ( XM_005174054.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174111.1 Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27190
Clone ID Related Accession (Same CDS sequence)	XM_005174054.3 , XM_005174054.4
Accession Version	XM_005174054.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serum response factor a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174054.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGT GGAGCGATGC CTCAACAGCT GCAAGCCATC 
CAGGTTCATC CCAGTCCTCA GAGCAGCGAC AGCAGTCCTG AGATCAGCCA GACGTCCAGC 
AGCGCCACTG CTAGCCACAC CGCGACTATA GTCACGTCCT CAGTGCCTTC TTCAGTGGCC 
GGACACATGA TGTACCCTGG AGCTCACGTC ATGTACGCCA CCTCCACCCC CGCCCTTACT 
GACGGAGGTC TGACTGTGCT CAGCGCCTTC CCACAACCCC CCAGTGCAAT GCATGTGTCG 
CACACACCGA ATCAGGACTC AGGTGGAGTT CCTCAGATGT TCCTCACAGG ACCTCCTGGC 
ACTGTACAGA TCCCAGTTTC AGCCGTTCCC CTTCATTCGA TGTTAATAAA CCAGCAGCCC 
TCTGGCAGCA GCAGTAGCAC CCTCACCGAG CTGCGCGTGG TCAATCTGGA CAATCCCAAA 
GGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174111.1
CDS	393..1841
Translation	MLGGNGTARGAGRSGNGTGLTPLQVGSTSGALGAVNSVINLPDGGRYDDGPSVEAVLSGSDGDSDSGDDEESPVPDRRGVKRERSELEVGGSSGSNAALSAGYGGVSSGVAGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRTDPSTDQRMSATGFEETDLTYQVSEADGLTEPKEMLKPTFTAATLPGGTNTISTTTQSPVSSSPSSSSVAMHTQSSGPSLSGTTCWPVAAGMNAQSISSANGTVLKSSSAGMMQLPSGFTLMPGGAMPQQLQAIQVHPSPQSSDSSPEISQTSSSATASHTATIVTSSVPSSVAGHMMYPGAHVMYATSTPALTDGGLTVLSAFPQPPSAMHVSHTPNQDSGGVPQMFLTGPPGTVQIPVSAVPLHSMLINQQPSGSSSSTLTELRVVNLDNPKGD

Target ORF information:

RefSeq Version	XM_005174054.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174054.4

ORF Insert Sequence:

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGT GGAGCGATGC CTCAACAGCT GCAAGCCATC 
CAGGTTCATC CCAGTCCTCA GAGCAGCGAC AGCAGTCCTG AGATCAGCCA GACGTCCAGC 
AGCGCCACTG CTAGCCACAC CGCGACTATA GTCACGTCCT CAGTGCCTTC TTCAGTGGCC 
GGACACATGA TGTACCCTGG AGCTCACGTC ATGTACGCCA CCTCCACCCC CGCCCTTACT 
GACGGAGGTC TGACTGTGCT CAGCGCCTTC CCACAACCCC CCAGTGCAAT GCATGTGTCG 
CACACACCGA ATCAGGACTC AGGTGGAGTT CCTCAGATGT TCCTCACAGG ACCTCCTGGC 
ACTGTACAGA TCCCAGTTTC AGCCGTTCCC CTTCATTCGA TGTTAATAAA CCAGCAGCCC 
TCTGGCAGCA GCAGTAGCAC CCTCACCGAG CTGCGCGTGG TCAATCTGGA CAATCCCAAA 
GGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srfa ( XM_017353104.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208593.1 Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.

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CloneID	ODa57018
Clone ID Related Accession (Same CDS sequence)	XM_017353104.1
Accession Version	XM_017353104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serum response factor a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGC GCTTCTCTTC CGCCGGGCAC ACACACTGTT 
CCTCTCAGTC AGCTGCAGAC ACACTCTCTG GCCATACAGT GTCCACAGAC GCAGGCCAAC 
AGCAGCGGCA GCACTGCTGT CCCCGCACAC ACACAACCCG CCGCGCTCTT CCGCCTGCCA 
GCCGCCGTCT CACTCACAGG TGGAGCGATG CCTCAACAGC TGCAAGCCAT CCAGGTTCAT 
CCCAGTCCTC AGAGCAGCGA CAGCAGTCCT GAGATCAGCC AGACGTCCAG CAGCGCCACT 
GCTAGCCACA CCGCGACTAT AGTCACGTCC TCAGTGCCTT CTTCAGTGGC CGGACACATG 
ATGTACCCTG GAGCTCACGT CATGTACGCC ACCTCCACCC CCGCCCTTAC TGACGGAGGT 
CTGACTGTGC TCAGCGCCTT CCCACAACCC CCCAGTGCAA TGCATGTGTC GCACACACCG 
AATCAGGACT CAGGTGGAGT TCCTCAGATG TTCCTCACAG GACCTCCTGG CACTGTACAG 
ATCCCAGTTT CAGCCGTTCC CCTTCATTCG GTAATAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208593.1
CDS	381..1922
Translation	MLGGNGTARGAGRSGNGTGLTPLQVGSTSGALGAVNSVINLPDGGRYDDGPSVEAVLSGSDGDSDSGDDEESPVPDRRGVKRERSELEVGGSSGSNAALSAGYGGVSSGVAGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRTDPSTDQRMSATGFEETDLTYQVSEADGLTEPKEMLKPTFTAATLPGGTNTISTTTQSPVSSSPSSSSVAMHTQSSGPSLSGTTCWPVAAGMNAQSISSANGTVLKSSSAGMMQLPSGFTLMPGASLPPGTHTVPLSQLQTHSLAIQCPQTQANSSGSTAVPAHTQPAALFRLPAAVSLTGGAMPQQLQAIQVHPSPQSSDSSPEISQTSSSATASHTATIVTSSVPSSVAGHMMYPGAHVMYATSTPALTDGGLTVLSAFPQPPSAMHVSHTPNQDSGGVPQMFLTGPPGTVQIPVSAVPLHSVIK

Target ORF information:

RefSeq Version	XM_017353104.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio serum response factor a (srfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353104.1

ORF Insert Sequence:

ATGTTAGGGG GCAACGGGAC GGCCAGAGGG GCTGGACGCT CTGGTAATGG CACGGGATTG 
ACACCTCTAC AGGTGGGCAG CACATCTGGG GCTCTGGGGG CTGTAAACTC TGTCATAAAC 
CTGCCCGATG GGGGCAGATA CGACGACGGG CCGTCGGTCG AGGCTGTGCT GAGCGGCTCC 
GATGGAGACT CAGACTCCGG GGACGATGAA GAGAGCCCTG TGCCGGACAG GAGAGGGGTG 
AAGCGGGAGA GGAGCGAGCT GGAGGTCGGG GGATCTTCTG GATCTAACGC CGCACTCTCG 
GCAGGATACG GAGGGGTCTC CAGCGGGGTG GCCGGAGCGA AGCCCGGCAA GAAAACGCGA 
GGACGGGTGA AAATTAAGAT GGAGTTTATT GACAACAAGC TGAGGAGATA CACGACCTTC 
AGCAAGAGAA AGACTGGCAT CATGAAGAAG GCCTACGAAC TCTCTACGCT GACAGGGACA 
CAAGTTTTAC TCTTGGTTGC CAGTGAAACG GGCCACGTCT ACACATTTGC CACGCGGAAA 
CTTCAACCCA TGATCACCAG TGAGACGGGT AAAGCCCTGA TCCAGACTTG CCTGAATTCC 
CCCGATTCTC CACCCCGTAC GGACCCTTCA ACAGACCAGC GCATGAGTGC CACGGGATTC 
GAGGAGACCG ACCTCACCTA CCAGGTCTCA GAGGCTGATG GCCTCACAGA GCCAAAGGAA 
ATGCTAAAGC CTACATTCAC TGCTGCCACT TTACCCGGAG GCACCAACAC CATCAGCACC 
ACCACACAGT CACCGGTCTC TTCCTCGCCC TCCTCATCCT CAGTGGCCAT GCACACCCAG 
AGCAGCGGCC CCTCGCTCTC TGGCACCACC TGCTGGCCGG TGGCGGCGGG GATGAACGCT 
CAGAGCATCT CCAGCGCAAA CGGCACCGTG CTCAAAAGTT CTTCAGCCGG CATGATGCAG 
CTGCCCAGCG GATTCACACT CATGCCAGGC GCTTCTCTTC CGCCGGGCAC ACACACTGTT 
CCTCTCAGTC AGCTGCAGAC ACACTCTCTG GCCATACAGT GTCCACAGAC GCAGGCCAAC 
AGCAGCGGCA GCACTGCTGT CCCCGCACAC ACACAACCCG CCGCGCTCTT CCGCCTGCCA 
GCCGCCGTCT CACTCACAGG TGGAGCGATG CCTCAACAGC TGCAAGCCAT CCAGGTTCAT 
CCCAGTCCTC AGAGCAGCGA CAGCAGTCCT GAGATCAGCC AGACGTCCAG CAGCGCCACT 
GCTAGCCACA CCGCGACTAT AGTCACGTCC TCAGTGCCTT CTTCAGTGGC CGGACACATG 
ATGTACCCTG GAGCTCACGT CATGTACGCC ACCTCCACCC CCGCCCTTAC TGACGGAGGT 
CTGACTGTGC TCAGCGCCTT CCCACAACCC CCCAGTGCAA TGCATGTGTC GCACACACCG 
AATCAGGACT CAGGTGGAGT TCCTCAGATG TTCCTCACAG GACCTCCTGG CACTGTACAG 
ATCCCAGTTT CAGCCGTTCC CCTTCATTCG GTAATAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ferulic Acid Promotes Hypertrophic Growth of Fast Skeletal Muscle in Zebrafish Model.
Nutrients9(10)(2017 Sep)
Wen Y,Ushio H

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Discovering miRNA Regulatory Networks in Holt-Oram Syndrome Using a Zebrafish Model.
Frontiers in bioengineering and biotechnology460(2016)
D'Aurizio R,Russo F,Chiavacci E,Baumgart M,Groth M,D'Onofrio M,Arisi I,Rainaldi G,Pitto L,Pellegrini M

MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome.
Scientific reports518240(2015 Dec)
Chiavacci E,D'Aurizio R,Guzzolino E,Russo F,Baumgart M,Groth M,Mariani L,D'Onofrio M,Arisi I,Pellegrini M,Cellerino A,Cremisi F,Pitto L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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srfa cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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