ctnnb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ctnnb1
Entrez Gene ID	30265
Full Name	catenin (cadherin-associated protein), beta 1
Synonyms	ctnnb,id:ibd2058,wu:fb73e10,wu:fi81c06,wu:fk25h01
General protein information	Preferred Names catenin (cadherin-associated protein), beta 1 Names catenin beta-1 beta-catenin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351314.1	XP_017206803.1	catenin beta-1 isoform X2
NM_131059.2	NP_571134.2	catenin beta-1
XM_005157831.3	XP_005157888.1	catenin beta-1 isoform X1
XM_005157831.4	XP_005157888.1	catenin beta-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CTNNB1	XP_001138023.1
Bos taurus (cattle)	CTNNB1	NP_001069609.1
Danio rerio (zebrafish)	ctnnb1	NP_571134.2
Mus musculus (house mouse)	Ctnnb1	NP_001159374.1
Rattus norvegicus (Norway rat)	Ctnnb1	NP_445809.2
Gallus gallus (chicken)	CTNNB1	NP_990412.1
Homo sapiens (human)	CTNNB1	NP_001091679.1
Drosophila melanogaster (fruit fly)	arm	NP_996328.1
Anopheles gambiae (African malaria mosquito)	ARM_ANOGA	XP_309245.5
Caenorhabditis elegans (roundworm)	hmp-2	NP_001252426.1
Canis lupus familiaris (dog)	CTNNB1	NP_001131124.1
	CTNNB1	XP_002802886.1
Xenopus tropicalis (tropical clawed frog)	ctnnb1	NP_001016958.1

Gene Ontology
Bibliography

Related articles in PubMed

A noncanonical sequence phosphorylated by casein kinase 1 in beta-catenin may play a role in casein kinase 1 targeting of important signaling proteins.
Marin O, Bustos VH, Cesaro L, Meggio F, Pagano MA, Antonelli M, Allende CC, Pinna LA, Allende JE
Proceedings of the National Academy of Sciences of the United States of America100(18)10193-200(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wnt/beta-catenin signaling induces expression of col12a1a/b and deposition of Collagen XII, which is necessary for axons to actively navigate the non-neural lesion site environment.
Title: Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish.

Net1-regulated beta-catenin activation plays a crucial role in the dorsal axis formation during zebrafish development.
Title: The guanine nucleotide exchange factor Net1 facilitates the specification of dorsal cell fates in zebrafish embryos by promoting maternal ?-catenin activation.

Nuclear beta-catenin is an indication of an activated Wnt pathway, therefore suggesting a possible role for Wnt signalling during zebrafish tooth development and replacement.
Title: Beta-Catenin and Plakoglobin Expression during Zebrafish Tooth Development and Replacement.

Mechanistically, Hif-3alpha2 binds to beta-catenin and destabilizes the nuclear beta-catenin complex.
Title: An oxygen-insensitive Hif-3? isoform inhibits Wnt signaling by destabilizing the nuclear ?-catenin complex.

Custos binds to beta-catenin in a Wnt responsive manner without affecting its stability, but rather modulates the cytoplasmic to nuclear translocation of beta-catenin.
Title: Custos controls ?-catenin to regulate head development during vertebrate embryogenesis.

The following ctnnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ctnnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01340	NM_131059.2 Latest version!	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa21057	XM_005157831.4 Latest version!	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa55362	XM_017351314.1 Latest version!	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa21057	XM_005157831.3	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ctnnb1 ( NM_131059.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571134.2 Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), mRNA.

Price & Availability↑

CloneID	ODa01340
Clone ID Related Accession (Same CDS sequence)	NM_131059.2
Accession Version	NM_131059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Danio rerio(zebrafish)
Product	catenin beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC047815.1. On Dec 20, 2003 this sequence version replaced NM_131059.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047815.1, U41081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATACACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAGTGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACAAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGGAGGGCAT GGAGGGGCTC 
TTGGGCACCC TGGTGCAGTT GCTCGGGTCA GATGACATCA ACGTGGTGAC GTGTGCTGCT 
GGAATCCTGT CCAACCTGAC CTGCAACAAC TACAAAAACA AGATGATGGT GTGCCAAGTG 
GGTGGCATCG AGGCCCCCGT GCGCACCGTT CTTCGTGCCG GAGACAGAGA GGATATCACA 
GAGCCGGCTA TCTGCGCTCT CCGTCACCTC ACATCCAGAC ACCAGGATGC AGAGATGGCC 
CAGAATGCAG TGCGGCTGCA CTATGGACTG CCTGTAGTAG TCAAACTGCT CCACCCTCCT 
TCACATTGGC CTCTCATTAA GGCTACAGTT GGGCTGATCC GCAACCTGGC ACTGTGCCCC 
GCCAACCATG CCCCGCTCCG AGAGCAGGGT GCCATTCCCC GCCTGGTGCA GCTTCTAGTC 
CGGGCCCATC AGGATACTCA GAGACGCACC TCCATGGGTG GAACACAGCA GCAGTTTGTG 
GAGGGTGTGC GTATGGAGGA GATTGTGGAG GGCTGCACTG GAGCTCTGCA CATTCTAGCC 
AGAGACATTC ACAACAGAAT TGTCATCAGA GGACTCAACA CTATTCCACT CTTTGTTCAG 
TTGCTGTATT CTCCTATCGA GAACATCCAG CGTGTGGCTG CAGGAGTGCT GTGTGAACTG 
GCTCAGGATA AGGAGGCGGC AGAAGCCATC GAGGCAGAAG GAGCCACTGC TCCACTCACA 
GAGCTCCTGC ACTCCAGAAA CGAGGGAGTC GCCACGTACG CTGCTGCAGT TCTGTTCCGC 
ATGTCTGAGG ATAAGCCCCA GGACTACAAG AAGCGACTGT CTGTGGAGCT CACCAGCTCT 
CTGTTCAGAA CTGAGCCCAT GACCTGGAAC GAGACTGGAG ATCTAGGGCT GGATATCGGT 
GCACAGGGAG AGCCTCTGGG CTACAGACAG GACGACCCAA GCTACCGCTC CTTCCATTCC 
GGAGGTTATG GGCAGGACGC CATGGGCATG GACCCCATGA TGGAGCACGA GATGGCCGGG 
CACCACCCGG GCCCTGATTA CCCCGTAGAC GGCCTGCCTG ACCTCGGCCA CACCCAGGAC 
CTGATCGACG GCCTCCCGCC AGGCGACAGC AATCAGCTGG CCTGGTTTGA CACCGATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571134.2
CDS	196..2538
Translation	MATQSDLMELEMAMDPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEDDDVDNQVLYEWEQGFNQSFNQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDSAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTSGTLHNLSHHREGLLAIFKSGGIPALVKVLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEAPVRTVLRAGDREDITEPAICALRHLTSRHQDAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDIHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMTWNETGDLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDAMGMDPMMEHEMAGHHPGPDYPVDGLPDLGHTQDLIDGLPPGDSNQLAWFDTDL

Target ORF information:

RefSeq Version	NM_131059.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131059.2

ORF Insert Sequence:

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATACACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAGTGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACAAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGGAGGGCAT GGAGGGGCTC 
TTGGGCACCC TGGTGCAGTT GCTCGGGTCA GATGACATCA ACGTGGTGAC GTGTGCTGCT 
GGAATCCTGT CCAACCTGAC CTGCAACAAC TACAAAAACA AGATGATGGT GTGCCAAGTG 
GGTGGCATCG AGGCCCCCGT GCGCACCGTT CTTCGTGCCG GAGACAGAGA GGATATCACA 
GAGCCGGCTA TCTGCGCTCT CCGTCACCTC ACATCCAGAC ACCAGGATGC AGAGATGGCC 
CAGAATGCAG TGCGGCTGCA CTATGGACTG CCTGTAGTAG TCAAACTGCT CCACCCTCCT 
TCACATTGGC CTCTCATTAA GGCTACAGTT GGGCTGATCC GCAACCTGGC ACTGTGCCCC 
GCCAACCATG CCCCGCTCCG AGAGCAGGGT GCCATTCCCC GCCTGGTGCA GCTTCTAGTC 
CGGGCCCATC AGGATACTCA GAGACGCACC TCCATGGGTG GAACACAGCA GCAGTTTGTG 
GAGGGTGTGC GTATGGAGGA GATTGTGGAG GGCTGCACTG GAGCTCTGCA CATTCTAGCC 
AGAGACATTC ACAACAGAAT TGTCATCAGA GGACTCAACA CTATTCCACT CTTTGTTCAG 
TTGCTGTATT CTCCTATCGA GAACATCCAG CGTGTGGCTG CAGGAGTGCT GTGTGAACTG 
GCTCAGGATA AGGAGGCGGC AGAAGCCATC GAGGCAGAAG GAGCCACTGC TCCACTCACA 
GAGCTCCTGC ACTCCAGAAA CGAGGGAGTC GCCACGTACG CTGCTGCAGT TCTGTTCCGC 
ATGTCTGAGG ATAAGCCCCA GGACTACAAG AAGCGACTGT CTGTGGAGCT CACCAGCTCT 
CTGTTCAGAA CTGAGCCCAT GACCTGGAAC GAGACTGGAG ATCTAGGGCT GGATATCGGT 
GCACAGGGAG AGCCTCTGGG CTACAGACAG GACGACCCAA GCTACCGCTC CTTCCATTCC 
GGAGGTTATG GGCAGGACGC CATGGGCATG GACCCCATGA TGGAGCACGA GATGGCCGGG 
CACCACCCGG GCCCTGATTA CCCCGTAGAC GGCCTGCCTG ACCTCGGCCA CACCCAGGAC 
CTGATCGACG GCCTCCCGCC AGGCGACAGC AATCAGCTGG CCTGGTTTGA CACCGATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ctnnb1 ( XM_005157831.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157888.1 Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21057
Clone ID Related Accession (Same CDS sequence)	XM_005157831.3 , XM_005157831.4
Accession Version	XM_005157831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	catenin beta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GACCCAAGCT ACCGCTCCTT CCATTCCGGA GGTTATGGGC AGGACGCCAT GGGAATGGAC 
CCCATGATGG AGCACGAGAT GGCCGGGCAC CACCCGGGCC CTGATTACCC CGTAGACGGC 
CTGCCTGACC TCGGCCACAC CCAGGACCTG ATCGACGGCC TCCCGCCAGG CGACAGCAAT 
CAGCTGGCCT GGTTTGACAC CGATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157888.1
CDS	228..2597
Translation	MATQSDLMELEMAMDPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEDDDVDNQVLYEWEQGFNQSFNQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDSAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTSGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQTSLAEVDVQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQDAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDIHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMTWNETGDLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDAMGMDPMMEHEMAGHHPGPDYPVDGLPDLGHTQDLIDGLPPGDSNQLAWFDTDL

Target ORF information:

RefSeq Version	XM_005157831.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157831.3

ORF Insert Sequence:

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GACCCAAGCT ACCGCTCCTT CCATTCCGGA GGTTATGGGC AGGACGCCAT GGGAATGGAC 
CCCATGATGG AGCACGAGAT GGCCGGGCAC CACCCGGGCC CTGATTACCC CGTAGACGGC 
CTGCCTGACC TCGGCCACAC CCAGGACCTG ATCGACGGCC TCCCGCCAGG CGACAGCAAT 
CAGCTGGCCT GGTTTGACAC CGATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ctnnb1 ( XM_005157831.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157888.1 Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.

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CloneID	ODa21057
Clone ID Related Accession (Same CDS sequence)	XM_005157831.3 , XM_005157831.4
Accession Version	XM_005157831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	catenin beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GACCCAAGCT ACCGCTCCTT CCATTCCGGA GGTTATGGGC AGGACGCCAT GGGAATGGAC 
CCCATGATGG AGCACGAGAT GGCCGGGCAC CACCCGGGCC CTGATTACCC CGTAGACGGC 
CTGCCTGACC TCGGCCACAC CCAGGACCTG ATCGACGGCC TCCCGCCAGG CGACAGCAAT 
CAGCTGGCCT GGTTTGACAC CGATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157888.1
CDS	227..2596
Translation	MATQSDLMELEMAMDPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEDDDVDNQVLYEWEQGFNQSFNQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDSAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTSGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQTSLAEVDVQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQDAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDIHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMTWNETGDLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDAMGMDPMMEHEMAGHHPGPDYPVDGLPDLGHTQDLIDGLPPGDSNQLAWFDTDL

Target ORF information:

RefSeq Version	XM_005157831.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157831.4

ORF Insert Sequence:

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GACCCAAGCT ACCGCTCCTT CCATTCCGGA GGTTATGGGC AGGACGCCAT GGGAATGGAC 
CCCATGATGG AGCACGAGAT GGCCGGGCAC CACCCGGGCC CTGATTACCC CGTAGACGGC 
CTGCCTGACC TCGGCCACAC CCAGGACCTG ATCGACGGCC TCCCGCCAGG CGACAGCAAT 
CAGCTGGCCT GGTTTGACAC CGATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ctnnb1 ( XM_017351314.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206803.1 Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X2, mRNA.

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CloneID	ODa55362
Clone ID Related Accession (Same CDS sequence)	XM_017351314.1
Accession Version	XM_017351314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	catenin beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GCTGTATCGT CTGATCTGAA TGAACCTGCA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206803.1
CDS	229..2424
Translation	MATQSDLMELEMAMDPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEDDDVDNQVLYEWEQGFNQSFNQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDSAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTSGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQTSLAEVDVQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQDAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDIHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMTWNETGDLGLDIGAQGEPLGYRQDAVSSDLNEPAL

Target ORF information:

RefSeq Version	XM_017351314.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio catenin (cadherin-associated protein), beta 1 (ctnnb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351314.1

ORF Insert Sequence:

ATGGCTACCC AGTCTGACTT GATGGAGCTG GAAATGGCCA TGGATCCGGA TCGCAAGGCT 
GCAGTCAGCC ATTGGCAGCA ACAGTCTTAC CTGGACTCAG GAATCCACTC TGGGGCCACA 
ACTACTGCCC CGTCCCTGAG TGGCAAAGGC AACCCGGAGG ATGACGATGT GGATAATCAG 
GTGCTTTATG AGTGGGAGCA GGGCTTCAAC CAGTCCTTCA ACCAAGAGCA AGTAGCAGAC 
ATCGATGGTC AATACGCCAT GACCAGAGCC CAGAGAGTCC GGGCGGCCAT GTTCCCGGAG 
ACTCTCGATG AGGGCATGCA GATACCTTCC ACACAGTTCG ACTCTGCGCA TCCCACCAAT 
GTCCAGCGCC TGGCTGAGCC CTCGCAGATG CTCAAACATG CCGTGGTCAA CCTCATCAAC 
TACCAGGACG ACGCAGAGCT GGCCACTCGT GCCATTCCAG AGCTCACCAA ACTACTGAAC 
GACGAGGATC AGGTGGTGGT TAATAAGGCA GCTGTGATGG TGCACCAGCT CTCCAAGAAA 
GAGGCCTCTC GTCACGCCAT CATGCGCTCC CCACAGATGG TATCGGCCAT TGTGAGGACC 
ATGCAGAACA CTAATGATGT AGAAACAGCC CGTTGCACCT CTGGCACCCT ACACAATCTT 
TCCCACCACA GAGAAGGACT GCTTGCCATC TTTAAATCAG GAGGCATCCC TGCCCTCGTC 
AAAATGCTCG GCTCCCCTGT GGACTCTGTG CTGTTTTACG CTATTACGAC TCTACACAAC 
CTGCTACTGC ACCAAGAAGG AGCCAAGATG GCTGTTCGAC TGGCCGGAGG CCTGCAGAAA 
ATGGTGGCCT TGTTAAACAA AACAAACGTC AAATTCCTCG CCATCACGAC AGACTGCCTT 
CAGATTCTGG CATACGGCAA TCAGGAAAGC AAACTTATCA TTCTGGCCAG CGGAGGCCCA 
CAGGCTTTGG TCAACATCAT GAGGACATAC ACCTATGAGA AGCTGTTATG GACGACCAGT 
CGAGTGCTCA AGGTGTTGTC AGTGTGCTCC AGTAACAAAC CTGCCATTGT GGAGGCTGGT 
GGCATGCAAG CCCTCGGCCT TCATCTCACA GACCCCAGCC AGCGACTGGT ACAGAACTGC 
TTGTGGACTT TGAGGAATCT GTCAGACGCC GCCACCAAAC AGACGTCTTT AGCAGAAGTA 
GATGTGCAGG AGGGCATGGA GGGGCTCTTG GGCACCCTGG TGCAGTTGCT CGGGTCAGAT 
GACATCAACG TGGTGACGTG TGCTGCTGGA ATCCTGTCCA ACCTGACCTG CAACAACTAC 
AAAAACAAGA TGATGGTGTG CCAAGTGGGT GGCATCGAGG CCCTCGTGCG CACCGTTCTT 
CGTGCCGGAG ACAGAGAGGA TATCACAGAG CCGGCTATCT GCGCTCTCCG TCACCTCACA 
TCCAGACACC AGGATGCAGA GATGGCCCAG AATGCAGTGC GGCTGCACTA TGGACTGCCT 
GTAGTAGTCA AACTGCTCCA CCCTCCTTCA CATTGGCCTC TCATTAAGGC TACAGTCGGG 
CTGATCCGCA ACCTGGCACT GTGCCCCGCC AACCATGCCC CGCTCCGAGA GCAGGGTGCC 
ATTCCCCGCC TGGTGCAGCT TCTAGTCCGG GCCCATCAGG ACACTCAGAG ACGCACCTCC 
ATGGGTGGAA CACAGCAGCA GTTTGTGGAG GGTGTGCGTA TGGAGGAGAT TGTGGAGGGC 
TGCACTGGAG CTCTGCACAT TCTAGCCAGA GACATTCACA ACAGAATCGT CATCAGAGGA 
CTCAACACTA TTCCACTCTT TGTTCAGTTG CTGTATTCTC CTATCGAGAA CATCCAGCGT 
GTGGCTGCAG GAGTGCTGTG TGAACTGGCT CAGGATAAGG AGGCGGCAGA AGCCATCGAG 
GCAGAAGGAG CCACTGCTCC ACTCACAGAG CTCCTGCACT CCAGAAACGA GGGAGTCGCC 
ACGTACGCTG CTGCAGTTCT GTTCCGCATG TCTGAGGATA AGCCCCAGGA CTACAAGAAG 
CGACTGTCTG TGGAGCTCAC CAGCTCTCTG TTCAGAACTG AGCCCATGAC CTGGAACGAG 
ACTGGAGATC TAGGGCTGGA TATCGGTGCA CAGGGAGAGC CTCTGGGCTA CAGACAGGAC 
GCTGTATCGT CTGATCTGAA TGAACCTGCA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A noncanonical sequence phosphorylated by casein kinase 1 in beta-catenin may play a role in casein kinase 1 targeting of important signaling proteins.
Proceedings of the National Academy of Sciences of the United States of America100(18)10193-200(2003 Sep)
Marin O,Bustos VH,Cesaro L,Meggio F,Pagano MA,Antonelli M,Allende CC,Pinna LA,Allende JE

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ctnnb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム