ntn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ntn1a
Entrez Gene ID	30227
Full Name	netrin 1a
Synonyms	net2,ntn2,zgc:136732
General protein information	Preferred Names netrin 1a Names netrin-1 net1a netrin 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476776.1	XP_021332451.1	netrin-1 isoform X1
NM_131029.2	NP_571104.1	netrin-1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ntn1	NP_446183.2
Homo sapiens (human)	NTN1	NP_004813.2
Canis lupus familiaris (dog)	NTN1	XP_850304.2
Mus musculus (house mouse)	Ntn1	NP_032770.2
Gallus gallus (chicken)	NTN1	NP_990750.1
Bos taurus (cattle)	NTN1	NP_001179495.1
Danio rerio (zebrafish)	ntn1a	NP_571104.1
Drosophila melanogaster (fruit fly)	NetB	NP_511155.1
Caenorhabditis elegans (roundworm)	unc-6	NP_509165.1
	NTN1	XP_001113322.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000228	XP_310904.5
Pan troglodytes (chimpanzee)	NTN1	XP_511846.3

Gene Ontology
Bibliography

Related articles in PubMed

Brorin is required for neurogenesis, gliogenesis, and commissural axon guidance in the zebrafish forebrain.
Miyake A, Mekata Y, Fujibayashi H, Nakanishi K, Konishi M, Itoh N
PloS one12(4)e0176036(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Careful phenotyping of patients combined with molecular and functional studies in zebrafish identifed netrin-1 as a potential shared genetic factor for cardiac and thyroid congenital defects.
Title: Functional zebrafish studies based on human genotyping point to netrin-1 as a link between aberrant cardiovascular development and thyroid dysgenesis.

These findings implicate Netrins as the only known attractants for olfactory sensory neurons.
Title: Netrin/DCC signaling guides olfactory sensory axons to their correct location in the olfactory bulb.

Data show that zebrafish Netrin 1a directs Dcc-mediated axon guidance of motoneurons and that this neural guidance function is essential for lymphangiogenesis.
Title: Motoneurons are essential for vascular pathfinding.

Netrin 1a is an intermediate target-derived signal that causes two equivalent motoneurons to adopt distinct fates
Title: Netrin signaling breaks the equivalence between two identified zebrafish motoneurons revealing a new role of intermediate targets.

Netrin-1 acts as a survival factor for endothelial cells, blocking the proapoptotic effect of the dependence receptor UNC5B and its downstream death signaling effector, the serine/threonine kinase DAPK.
Title: Inhibition of endothelial cell apoptosis by netrin-1 during angiogenesis.

The following ntn1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ntn1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01065	NM_131029.2 Latest version!	Danio rerio netrin 1a (ntn1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa142916	XM_021476776.1 Latest version!	Danio rerio netrin 1a (ntn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ntn1a ( NM_131029.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571104.1 Danio rerio netrin 1a (ntn1a), mRNA.

Price & Availability↑

CloneID	ODa01065
Clone ID Related Accession (Same CDS sequence)	NM_131029.2
Accession Version	NM_131029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	netrin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX255899.5. On Jun 17, 2009 this sequence version replaced NM_131029.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01028812.1, AF002717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGAGAG TCTCTGATGC TTTGGTCACT TTGGTGACTC TCTGCTGTGT GCTCAAAGGG 
ACTGTCGGCG GATATGGAAT GAGCATGTTC GCCGCTCAGA CCTCCCCGCC GGATCCGTGT 
TACGACGAGA ACGGACACCC CAGAAGATGC ATCCCCGACT TCGTAAACGC GGCGTTCGGG 
AAAGAAGTAC GCGCGTCCAG CACCTGCGGC AAAACGCCGA GTCGTTACTG CGTGGTGACC 
GAGAAAGGGG ACGAAAGACA CAGAAACTGC CACACGTGCG ACGCGTCAGA CCCAAAGAAG 
AATCACCCAC CAGCTTACCT GACCGACCTG AACAATCCTC ACAATCTCAC CTGCTGGCAG 
TCGGACAATT ACCTCCAGTA TCCTCAAAAC GTCACTTTAA CTTTATCCTT GGGCAAGAAA 
TTTGAGGTGA CCTACGTGAG TTTGCAGTTC TGCTCACCTC GACCGGAGTC TATGGCGATC 
TTTAAATCGA TGGACTACGG AAAGTCCTGG GTGCCTTTCC AGTACTACTC GACCCAGTGT 
AGAAAGATGT ACAACAAGCC CAGCAAAGCC ACGATTACTA AGCAGAACGA GCAAGAGGCC 
ATCTGCACAG ATTCTCACAC CGACATGCAT CCTCTCTCCG GCGGGCTGAT CGCGTTCAGC 
ACCCTGGACG GGCGACCCTC CGCGCACGAC TTTGACAATT CACCCGTACT TCAGGACTGG 
GTGACCGCCA CTGACATTAA GGTGACTTTC AGCCGCCTGC ACACTTTCGG AGACGAAAAC 
GAGGATGACT CGGAGCTGGC CAGAGATTCC TATTTTTACG CAGTTTCCGA CCTGCAGGTT 
GGAGGCAGAT GTAAGTGTAA TGGACACGCA TCACGGTGCG TCAAAGACCG GGATGGAAAC 
CTAGTGTGCG AGTGCAAGCA CAACACAGCC GGACCAGAGT GTGACAGATG CAAACCTTTT 
CACTATGACC GACCCTGGCA GCGCGCAACC GCCAGAGAAG CCAACGAATG TGTCGCCTGC 
AATTGTAACC TTCATGCGAG GCGCTGTCGT TTCAACATGG AGCTTTACAA ACTCTCTGGA 
AGGAAAAGTG GAGGAGTCTG TCTGAACTGC CGCCACAATA CAGCTGGTCG CCACTGCCAC 
TACTGCAAAG AGGGCTACTA TAGAGACATG TCCAAGCCCA TCTCCCACAG AAAGGCCTGC 
AAAGCCTGTG ATTGCCATCC TGTGGGGGCC GCGGGCAAAA CCTGTAACCA AACCACAGGC 
CAATGCCCCT GTAAAGACGG TGTGACGGGT ATCACATGCA ACCGTTGTGC TAACGGCTAC 
CAGCAGAGCC GATCACCCAT TGCCCCCTGC ATAAAAATTC CCATCGCTCC GCCAACCACC 
ACTGCAAGCA GCACAGAAGA GCCATCAGAC TGTGAATCCT ACTGCAAGGC ATCCAAAGGC 
AAGCTGAAGA TCAATATGAA GAAGTACTGC AAGAAAGATT ATGCCGTTCA AGTCCACATC 
CTGAAAGCAG ATAAAGCAGG AGAGTGGTGG AAGTTCACCG TCAACATCAT CTCTGTTTAC 
AAACAGGGTG AAAGCCGAAT TCGCAGAGGA GACCAGTTCC TCTGGGTCAG GGCAAAGGAT 
GTGGCCTGCA AGTGTCCGAA GATCAAGTCC GGCAAGAAAT ACCTTCTGCT GGGGAACGAC 
GAGGATTCGC CAGGACAAAG CGGAATGGTG GCGGACAAGG GCAGTCTGGT CATTCAGTGG 
AGAGACACTT GGGCTCGGAG ACTCCGGAAG TTTCAGCAAA GGGAGAAGAA AGGAAAATGC 
AAGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571104.1
CDS	508..2319
Translation	MLRVSDALVTLVTLCCVLKGTVGGYGMSMFAAQTSPPDPCYDENGHPRRCIPDFVNAAFGKEVRASSTCGKTPSRYCVVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYPQNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKSWVPFQYYSTQCRKMYNKPSKATITKQNEQEAICTDSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPCIKIPIAPPTTTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRRGDQFLWVRAKDVACKCPKIKSGKKYLLLGNDEDSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKKA

Target ORF information:

RefSeq Version	NM_131029.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio netrin 1a (ntn1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131029.2

ORF Insert Sequence:

ATGTTGAGAG TCTCTGATGC TTTGGTCACT TTGGTGACTC TCTGCTGTGT GCTCAAAGGG 
ACTGTCGGCG GATATGGAAT GAGCATGTTC GCCGCTCAGA CCTCCCCGCC GGATCCGTGT 
TACGACGAGA ACGGACACCC CAGAAGATGC ATCCCCGACT TCGTAAACGC GGCGTTCGGG 
AAAGAAGTAC GCGCGTCCAG CACCTGCGGC AAAACGCCGA GTCGTTACTG CGTGGTGACC 
GAGAAAGGGG ACGAAAGACA CAGAAACTGC CACACGTGCG ACGCGTCAGA CCCAAAGAAG 
AATCACCCAC CAGCTTACCT GACCGACCTG AACAATCCTC ACAATCTCAC CTGCTGGCAG 
TCGGACAATT ACCTCCAGTA TCCTCAAAAC GTCACTTTAA CTTTATCCTT GGGCAAGAAA 
TTTGAGGTGA CCTACGTGAG TTTGCAGTTC TGCTCACCTC GACCGGAGTC TATGGCGATC 
TTTAAATCGA TGGACTACGG AAAGTCCTGG GTGCCTTTCC AGTACTACTC GACCCAGTGT 
AGAAAGATGT ACAACAAGCC CAGCAAAGCC ACGATTACTA AGCAGAACGA GCAAGAGGCC 
ATCTGCACAG ATTCTCACAC CGACATGCAT CCTCTCTCCG GCGGGCTGAT CGCGTTCAGC 
ACCCTGGACG GGCGACCCTC CGCGCACGAC TTTGACAATT CACCCGTACT TCAGGACTGG 
GTGACCGCCA CTGACATTAA GGTGACTTTC AGCCGCCTGC ACACTTTCGG AGACGAAAAC 
GAGGATGACT CGGAGCTGGC CAGAGATTCC TATTTTTACG CAGTTTCCGA CCTGCAGGTT 
GGAGGCAGAT GTAAGTGTAA TGGACACGCA TCACGGTGCG TCAAAGACCG GGATGGAAAC 
CTAGTGTGCG AGTGCAAGCA CAACACAGCC GGACCAGAGT GTGACAGATG CAAACCTTTT 
CACTATGACC GACCCTGGCA GCGCGCAACC GCCAGAGAAG CCAACGAATG TGTCGCCTGC 
AATTGTAACC TTCATGCGAG GCGCTGTCGT TTCAACATGG AGCTTTACAA ACTCTCTGGA 
AGGAAAAGTG GAGGAGTCTG TCTGAACTGC CGCCACAATA CAGCTGGTCG CCACTGCCAC 
TACTGCAAAG AGGGCTACTA TAGAGACATG TCCAAGCCCA TCTCCCACAG AAAGGCCTGC 
AAAGCCTGTG ATTGCCATCC TGTGGGGGCC GCGGGCAAAA CCTGTAACCA AACCACAGGC 
CAATGCCCCT GTAAAGACGG TGTGACGGGT ATCACATGCA ACCGTTGTGC TAACGGCTAC 
CAGCAGAGCC GATCACCCAT TGCCCCCTGC ATAAAAATTC CCATCGCTCC GCCAACCACC 
ACTGCAAGCA GCACAGAAGA GCCATCAGAC TGTGAATCCT ACTGCAAGGC ATCCAAAGGC 
AAGCTGAAGA TCAATATGAA GAAGTACTGC AAGAAAGATT ATGCCGTTCA AGTCCACATC 
CTGAAAGCAG ATAAAGCAGG AGAGTGGTGG AAGTTCACCG TCAACATCAT CTCTGTTTAC 
AAACAGGGTG AAAGCCGAAT TCGCAGAGGA GACCAGTTCC TCTGGGTCAG GGCAAAGGAT 
GTGGCCTGCA AGTGTCCGAA GATCAAGTCC GGCAAGAAAT ACCTTCTGCT GGGGAACGAC 
GAGGATTCGC CAGGACAAAG CGGAATGGTG GCGGACAAGG GCAGTCTGGT CATTCAGTGG 
AGAGACACTT GGGCTCGGAG ACTCCGGAAG TTTCAGCAAA GGGAGAAGAA AGGAAAATGC 
AAGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ntn1a ( XM_021476776.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332451.1 Danio rerio netrin 1a (ntn1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142916
Clone ID Related Accession (Same CDS sequence)	XM_021476776.1
Accession Version	XM_021476776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	netrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTCTGATGC TTTGGTCACT TTGGTGACTC TCTGCTGTGT GCTCAAAGGG 
ACTGTCGGCG GATATGGAAT GAGCATGTTC GCCGCTCAGA CCTCCCCGCC GGATCCGTGT 
TACGACGAGA ACGGACACCC CAGAAGATGC ATCCCCGACT TCGTAAACGC GGCGTTCGGG 
AAAGAAGTAC GCGCGTCCAG CACCTGCGGC AAAACGCCGA GTCGTTACTG CGTGGTGACC 
GAGAAAGGGG ACGAAAGACA CAGAAACTGC CACACGTGCG ACGCGTCAGA CCCAAAGAAG 
AATCACCCAC CAGCTTACCT GACCGACCTG AACAATCCTC ACAATCTCAC CTGCTGGCAG 
TCGGACAATT ACCTCCAGTA TCCTCAAAAC GTCACTTTAA CTTTATCCTT GGGCAAGAAA 
TTTGAGGTGA CCTACGTGAG TTTGCAGTTC TGCTCACCTC GACCGGAGTC TATGGCGATC 
TTTAAATCGA TGGACTACGG AAAGTCCTGG GTGCCTTTCC AGTACTACTC GACCCAGTGT 
AGAAAGATGT ACAACAAGCC CAGCAAAGCC ACGATTACTA AGCAGAACGA GCAAGAGGCC 
ATCTGCACAG ATTCTCACAC CGACATGCAT CCTCTCTCCG GCGGGCTGAT CGCGTTCAGC 
ACCCTGGACG GGCGACCCTC CGCGCACGAC TTTGACAATT CACCCGTACT TCAGGACTGG 
GTGACCGCCA CTGACATTAA GGTGACTTTC AGCCGCCTGC ACACTTTCGG AGACGAAAAC 
GAGGATGACT CGGAGCTGGC CAGAGATTCC TATTTTTACG CAGTTTCCGA CCTGCAGGTT 
GGAGGCAGAT GTAAGTGTAA TGGACACGCA TCACGGTGCG TCAAAGACCG GGATGGAAAC 
CTAGTGTGCG AGTGCAAGCA CAACACAGCC GGACCAGAGT GTGACAGATG CAAACCTTTT 
CACTATGACC GACCCTGGCA GCGCGCAACC GCCAGAGAAG CCAACGAATG TGTCGCCTGC 
AATTGTAACC TTCATGCGAG GCGCTGTCGT TTCAACATGG AGCTTTACAA ACTCTCTGGA 
AGGAAAAGTG GAGGAGTCTG TCTGAACTGC CGCCACAATA CAGCTGGTCG CCACTGCCAC 
TACTGCAAAG AGGGCTACTA TAGAGACATG TCCAAGCCCA TCTCCCACAG AAAGGCCTGC 
AAAGAAATTC CCATCGCTCC GCCAACCACC ACTGCAAGCA GCACAGAAGA GCCATCAGAC 
TGTGAATCCT ACTGCAAGGC ATCCAAAGGC AAGCTGAAGA TCAATATGAA GAAGTACTGC 
AAGAAAGATT ATGCCGTTCA AGTCCACATC CTGAAAGCAG ATAAAGCAGG AGAGTGGTGG 
AAGTTCACCG TCAACATCAT CTCTGTTTAC AAACAGGGTG AAAGCCGAAT TCGCAGAGGA 
GACCAGTTCC TCTGGGTCAG GGCAAAGGAT GTGGCCTGCA AGTGTCCGAA GATCAAGTCC 
GGCAAGAAAT ACCTTCTGCT GGGGAACGAC GAGGATTCGC CAGGACAAAG CGGAATGGTG 
GCGGACAAGG GCAGTCTGGT CATTCAGTGG AGAGACACTT GGGCTCGGAG ACTCCGGAAG 
TTTCAGCAAA GGGAGAAGAA AGGAAAATGC AAGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332451.1
CDS	502..2163
Translation	MLRVSDALVTLVTLCCVLKGTVGGYGMSMFAAQTSPPDPCYDENGHPRRCIPDFVNAAFGKEVRASSTCGKTPSRYCVVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYPQNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKSWVPFQYYSTQCRKMYNKPSKATITKQNEQEAICTDSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKEIPIAPPTTTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRRGDQFLWVRAKDVACKCPKIKSGKKYLLLGNDEDSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKKA

Target ORF information:

RefSeq Version	XM_021476776.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio netrin 1a (ntn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476776.1

ORF Insert Sequence:

ATGTTGAGAG TCTCTGATGC TTTGGTCACT TTGGTGACTC TCTGCTGTGT GCTCAAAGGG 
ACTGTCGGCG GATATGGAAT GAGCATGTTC GCCGCTCAGA CCTCCCCGCC GGATCCGTGT 
TACGACGAGA ACGGACACCC CAGAAGATGC ATCCCCGACT TCGTAAACGC GGCGTTCGGG 
AAAGAAGTAC GCGCGTCCAG CACCTGCGGC AAAACGCCGA GTCGTTACTG CGTGGTGACC 
GAGAAAGGGG ACGAAAGACA CAGAAACTGC CACACGTGCG ACGCGTCAGA CCCAAAGAAG 
AATCACCCAC CAGCTTACCT GACCGACCTG AACAATCCTC ACAATCTCAC CTGCTGGCAG 
TCGGACAATT ACCTCCAGTA TCCTCAAAAC GTCACTTTAA CTTTATCCTT GGGCAAGAAA 
TTTGAGGTGA CCTACGTGAG TTTGCAGTTC TGCTCACCTC GACCGGAGTC TATGGCGATC 
TTTAAATCGA TGGACTACGG AAAGTCCTGG GTGCCTTTCC AGTACTACTC GACCCAGTGT 
AGAAAGATGT ACAACAAGCC CAGCAAAGCC ACGATTACTA AGCAGAACGA GCAAGAGGCC 
ATCTGCACAG ATTCTCACAC CGACATGCAT CCTCTCTCCG GCGGGCTGAT CGCGTTCAGC 
ACCCTGGACG GGCGACCCTC CGCGCACGAC TTTGACAATT CACCCGTACT TCAGGACTGG 
GTGACCGCCA CTGACATTAA GGTGACTTTC AGCCGCCTGC ACACTTTCGG AGACGAAAAC 
GAGGATGACT CGGAGCTGGC CAGAGATTCC TATTTTTACG CAGTTTCCGA CCTGCAGGTT 
GGAGGCAGAT GTAAGTGTAA TGGACACGCA TCACGGTGCG TCAAAGACCG GGATGGAAAC 
CTAGTGTGCG AGTGCAAGCA CAACACAGCC GGACCAGAGT GTGACAGATG CAAACCTTTT 
CACTATGACC GACCCTGGCA GCGCGCAACC GCCAGAGAAG CCAACGAATG TGTCGCCTGC 
AATTGTAACC TTCATGCGAG GCGCTGTCGT TTCAACATGG AGCTTTACAA ACTCTCTGGA 
AGGAAAAGTG GAGGAGTCTG TCTGAACTGC CGCCACAATA CAGCTGGTCG CCACTGCCAC 
TACTGCAAAG AGGGCTACTA TAGAGACATG TCCAAGCCCA TCTCCCACAG AAAGGCCTGC 
AAAGAAATTC CCATCGCTCC GCCAACCACC ACTGCAAGCA GCACAGAAGA GCCATCAGAC 
TGTGAATCCT ACTGCAAGGC ATCCAAAGGC AAGCTGAAGA TCAATATGAA GAAGTACTGC 
AAGAAAGATT ATGCCGTTCA AGTCCACATC CTGAAAGCAG ATAAAGCAGG AGAGTGGTGG 
AAGTTCACCG TCAACATCAT CTCTGTTTAC AAACAGGGTG AAAGCCGAAT TCGCAGAGGA 
GACCAGTTCC TCTGGGTCAG GGCAAAGGAT GTGGCCTGCA AGTGTCCGAA GATCAAGTCC 
GGCAAGAAAT ACCTTCTGCT GGGGAACGAC GAGGATTCGC CAGGACAAAG CGGAATGGTG 
GCGGACAAGG GCAGTCTGGT CATTCAGTGG AGAGACACTT GGGCTCGGAG ACTCCGGAAG 
TTTCAGCAAA GGGAGAAGAA AGGAAAATGC AAGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Brorin is required for neurogenesis, gliogenesis, and commissural axon guidance in the zebrafish forebrain.
PloS one12(4)e0176036(2017)
Miyake A,Mekata Y,Fujibayashi H,Nakanishi K,Konishi M,Itoh N

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ntn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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