wnt4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnt4
Entrez Gene ID	30123
Full Name	wingless-type MMTV integration site family, member 4a
Synonyms	wnt-4,wnt4,zgc:136737
General protein information	Preferred Names wingless-type MMTV integration site family, member 4a Names protein Wnt-4a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040387.1	NP_001035477.1	protein Wnt-4a precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wnt4	NP_033549.1
Drosophila melanogaster (fruit fly)	Wnt5	NP_476924.1
Caenorhabditis elegans (roundworm)	cwn-1	NP_493668.1
Xenopus tropicalis (tropical clawed frog)	wnt4	NP_001239015.1
Homo sapiens (human)	WNT4	NP_110388.2
Pan troglodytes (chimpanzee)	WNT4	XP_524597.3
Bos taurus (cattle)	WNT4	XP_003585839.2
Rattus norvegicus (Norway rat)	Wnt4	NP_445854.1
Canis lupus familiaris (dog)	WNT4	XP_855190.3
Gallus gallus (chicken)	WNT4	NP_990114.1
Danio rerio (zebrafish)	wnt4a	NP_001035477.1
	WNT4	XP_001100814.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanisms of RhoA inactivation and CDC42 and Rac1 activation during zebrafish optic nerve regeneration.
Matsukawa T, Morita K, Omizu S, Kato S, Koriyama Y
Neurochemistry international11271-80(2018 Jan)

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
Lee SLJ, Horsfield JA, Black MA, Rutherford K, Fisher A, Gemmell NJ
BMC genomics18(1)557(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that in vivo, wnt11r and wnt4a initiate MuSK translocation from muscle membranes to recycling endosomes and that this transition is crucial for AChR accumulation at future synaptic sites.
Title: Initiation of synapse formation by Wnt-induced MuSK endocytosis.

Data show that injury-dependent induction of Ascl1a suppressed expression of the Wnt signaling inhibitor, Dkk, and induced expression of the Wnt ligand, Wnt4a.
Title: Ascl1a/Dkk/beta-catenin signaling pathway is necessary and glycogen synthase kinase-3beta inhibition is sufficient for zebrafish retina regeneration.

Findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo.
Title: Negative feedback regulation of Wnt4 signaling by EAF1 and EAF2/U19.

three Wnt noncanonical ligands wnt4a, silberblick/wnt11, and wnt11-related regulate the process of convergence of endoderm and organ precursors toward the embryonic midline by acting in a largely redundant way
Title: Noncanonical Wnt signaling regulates midline convergence of organ primordia during zebrafish development.

The following wnt4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnt4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00819	NM_001040387.1 Latest version!	Danio rerio wingless-type MMTV integration site family, member 4 (wnt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnt4 ( NM_001040387.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035477.1 Danio rerio wingless-type MMTV integration site family, member 4 (wnt4), mRNA.

Price & Availability↑

CloneID	ODa00819
Clone ID Related Accession (Same CDS sequence)	NM_001040387.1
Accession Version	NM_001040387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Danio rerio(zebrafish)
Product	protein Wnt-4a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC115247.1. On Apr 17, 2008 this sequence version replaced NM_130947.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115247.1, U51267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGTCATCGG AGTATTTGAT AAGGTCACTG CTTATGCTCT TTCTAGCGCT CTTCTCCGCC 
AACGCGAGCA ACTGGCTATA CCTGGCCAAG CTGTCGTCGG TGGGGAGCAT CTCTGATGAG 
GAGACGTGTG AGAAGCTGCG AGGACTCATC CAGAGGCAGG TTCAGATCTG TAAACGTAAT 
GTGGAGGTGA TGGATGCGGT GCGCAGAGGA GCTCAGCTCG CCATCGACGA GTGCCAGTAT 
CAGTTCCGCA ACCGCCGATG GAACTGCTCC ACGCTGGAGA GCGTTCCTGT GTTTGGGAAA 
GTGGTCACAC AAGGTACGAG GGAAGCTGCA TTTGTATATG CCATATCCGC AGCTAGCGTA 
GCATTTGCAG TGACCAGAGC CTGCAGCAGT GGAGAGCTGG ACAAGTGTGG ATGTGACCGT 
AATGTTCACG GAGTCAGTCC AGAAGGTTTC CAGTGGTCAG GATGCTCAGA TAACATTGCA 
TATGGGGTTG CTTTCTCCCA ATCCTTCGTG GACATCAGGG AACGGAGTAA AGGACAGTCA 
TCCAACAGAG CCCTAATGAA CCTTCATAAC AATGAAGCAG GAAGAAAAGC GATTCTCAAC 
CACATGAGAG TGGAGTGCAA ATGCCACGGC GTTTCAGGCT CCTGCGAAGT CAAGACCTGC 
TGGAAAGCGA TGCCGCCTTT CCGCAAAGTG GGCAACGTCA TAAAGGAGAA ATTCGACGGT 
GCCACAGAAG TGGAGCTGCG TAAAGTGGGC ACCACCAAAG TCCTGGTGCC CCGAAACTCA 
CAATTCAAGC CGCACACAGA CGAAGATCTG GTCTACCTGG ACCCGAGTCC AGACTTCTGC 
GAGCATGACC CCAGAACGCC CGGTATAATG GGCACGGCGG GCCGATTCTG CAACAAGACC 
TCCAAGGCCA TCGACGGCTG CGAGCTGATG TGCTGCGGTC GCGGTTTCCA CACGGAGGAG 
GTGGAGGTGG TGGACCGCTG CAGCTGCAAG TTCCACTGGT GCTGCTACGT CAAATGCAAA 
CAGTGTCGCA AGATGGTGGA GATGCACACG TGTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035477.1
CDS	344..1402
Translation	MSSEYLIRSLLMLFLALFSANASNWLYLAKLSSVGSISDEETCEKLRGLIQRQVQICKRNVEVMDAVRRGAQLAIDECQYQFRNRRWNCSTLESVPVFGKVVTQGTREAAFVYAISAASVAFAVTRACSSGELDKCGCDRNVHGVSPEGFQWSGCSDNIAYGVAFSQSFVDIRERSKGQSSNRALMNLHNNEAGRKAILNHMRVECKCHGVSGSCEVKTCWKAMPPFRKVGNVIKEKFDGATEVELRKVGTTKVLVPRNSQFKPHTDEDLVYLDPSPDFCEHDPRTPGIMGTAGRFCNKTSKAIDGCELMCCGRGFHTEEVEVVDRCSCKFHWCCYVKCKQCRKMVEMHTCR

Target ORF information:

RefSeq Version	NM_001040387.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wingless-type MMTV integration site family, member 4 (wnt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040387.1

ORF Insert Sequence:

ATGTCATCGG AGTATTTGAT AAGGTCACTG CTTATGCTCT TTCTAGCGCT CTTCTCCGCC 
AACGCGAGCA ACTGGCTATA CCTGGCCAAG CTGTCGTCGG TGGGGAGCAT CTCTGATGAG 
GAGACGTGTG AGAAGCTGCG AGGACTCATC CAGAGGCAGG TTCAGATCTG TAAACGTAAT 
GTGGAGGTGA TGGATGCGGT GCGCAGAGGA GCTCAGCTCG CCATCGACGA GTGCCAGTAT 
CAGTTCCGCA ACCGCCGATG GAACTGCTCC ACGCTGGAGA GCGTTCCTGT GTTTGGGAAA 
GTGGTCACAC AAGGTACGAG GGAAGCTGCA TTTGTATATG CCATATCCGC AGCTAGCGTA 
GCATTTGCAG TGACCAGAGC CTGCAGCAGT GGAGAGCTGG ACAAGTGTGG ATGTGACCGT 
AATGTTCACG GAGTCAGTCC AGAAGGTTTC CAGTGGTCAG GATGCTCAGA TAACATTGCA 
TATGGGGTTG CTTTCTCCCA ATCCTTCGTG GACATCAGGG AACGGAGTAA AGGACAGTCA 
TCCAACAGAG CCCTAATGAA CCTTCATAAC AATGAAGCAG GAAGAAAAGC GATTCTCAAC 
CACATGAGAG TGGAGTGCAA ATGCCACGGC GTTTCAGGCT CCTGCGAAGT CAAGACCTGC 
TGGAAAGCGA TGCCGCCTTT CCGCAAAGTG GGCAACGTCA TAAAGGAGAA ATTCGACGGT 
GCCACAGAAG TGGAGCTGCG TAAAGTGGGC ACCACCAAAG TCCTGGTGCC CCGAAACTCA 
CAATTCAAGC CGCACACAGA CGAAGATCTG GTCTACCTGG ACCCGAGTCC AGACTTCTGC 
GAGCATGACC CCAGAACGCC CGGTATAATG GGCACGGCGG GCCGATTCTG CAACAAGACC 
TCCAAGGCCA TCGACGGCTG CGAGCTGATG TGCTGCGGTC GCGGTTTCCA CACGGAGGAG 
GTGGAGGTGG TGGACCGCTG CAGCTGCAAG TTCCACTGGT GCTGCTACGT CAAATGCAAA 
CAGTGTCGCA AGATGGTGGA GATGCACACG TGTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanisms of RhoA inactivation and CDC42 and Rac1 activation during zebrafish optic nerve regeneration.
Neurochemistry international11271-80(2018 Jan)
Matsukawa T,Morita K,Omizu S,Kato S,Koriyama Y

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
BMC genomics18(1)557(2017 Jul)
Lee SLJ,Horsfield JA,Black MA,Rutherford K,Fisher A,Gemmell NJ

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wnt4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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