wnt8a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnt8a
Entrez Gene ID	30122
Full Name	wingless-type MMTV integration site family, member 8a
Synonyms	wnt-8,wnt8,wnt8.1,wu:fa20e02,wu:fe05d07
General protein information	Preferred Names wingless-type MMTV integration site family, member 8a Names protein Wnt-8a ORF1 etID309727.14
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a member of the conserved Wnt family of secreted signaling proteins. The encoded protein is essential for correct patterning of the mesoderm and neural ectoderm during gastrulation. In zebrafish, two similar, functional proteins are co-transcribed from a single bicistronic mRNA. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130946.3	NP_571021.3	protein Wnt-8a ORF1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	WNT8A	NP_490645.1
Canis lupus familiaris (dog)	WNT8A	XP_005626641.1
Mus musculus (house mouse)	Wnt8a	NP_033316.1
Rattus norvegicus (Norway rat)	Wnt8a	NP_001099625.1
Danio rerio (zebrafish)	wnt8a	NP_571021.3
	WNT8A	XP_001112480.1
Xenopus tropicalis (tropical clawed frog)	wnt8a	NP_001017208.1
Pan troglodytes (chimpanzee)	WNT8A	XP_527023.3
Bos taurus (cattle)	WNT8A	NP_001179299.1
Gallus gallus (chicken)	WNT8A	NP_990862.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles of maternal wnt8a transcripts in axis formation in zebrafish.
Hino H, Nakanishi A, Seki R, Aoki T, Yamaha E, Kawahara A, Shimizu T, Hibi M
Developmental biology(2017 Dec)

Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments.
Sezgin E, Azbazdar Y, Ng XW, Teh C, Simons K, Weidinger G, Wohland T, Eggeling C, Ozhan G
The FEBS journal284(15)2513-2526(2017 Aug)

Harmless effects of argon plasma on caudal fin regeneration and embryogenesis of zebrafish: novel biological approaches for safe medical applications of bioplasma.
Nam MK, Kim GY, Yun SE, Jang JY, Kim YH, Choi EH, Kang S, Rhim H
Experimental & molecular medicine49(7)e355(2017 Jul)

Wnt8a expands the pool of embryonic kidney progenitors in zebrafish.
Naylor RW, Han HI, Hukriede NA, Davidson AJ
Developmental biology425(2)130-141(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Wnt3, Wnt3a, and Wnt8a bind to their respective receptors (Fz8, Lrp6, and Lypd6) in ordered plasma membrane environments; ordered plasma membrane environments appear to be essential for binding of Wnt proteins to their receptor complexes and stimulation of downstream signaling activity.
Title: Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments.

Wnt8, in addition to its well-established role in posterior mesoderm patterning, also plays a later role as a factor that expands the renal progenitor pool prior to kidney morphogenesis.
Title: Wnt8a expands the pool of embryonic kidney progenitors in zebrafish.

Wnt signaling balances specification of the cardiac and pharyngeal muscle fields.
Title: Wnt signaling balances specification of the cardiac and pharyngeal muscle fields.

A filopodia-based transport system for Wnt8a controls anteroposterior patterning of the neural plate during vertebrate gastrulation.
Title: Filopodia-based Wnt transport during vertebrate tissue patterning.

Cortical depth and differential transport of vegetally localized dorsal and germ line determinants, Wnt8a, grip2a, and Dazl, in the zebrafish embryo have been presented.
Title: Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo.

The following wnt8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnt8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02056	NM_130946.3 Latest version!	Danio rerio wingless-type MMTV integration site family, member 8a (wnt8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnt8a ( NM_130946.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571021.3 Danio rerio wingless-type MMTV integration site family, member 8a (wnt8a), mRNA.

Price & Availability↑

CloneID	ODa02056
Clone ID Related Accession (Same CDS sequence)	NM_130946.3
Accession Version	NM_130946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Danio rerio(zebrafish)
Product	protein Wnt-8a ORF1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX927327.14 and BX248097.7. On May 1, 2013 this sequence version replaced NM_130946.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## bicistronic transcript :: PMID: 11703928 CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:11703928) NMD candidate :: PMID: 11703928 ##RefSeq-Attributes-END##

ATGAACCCTT GCCAGATTTT TGCGTCGTTG GTTATGTCTA TATGCTGTCA CATACTGTCG 
TCAACAGCAT GGTCTGTGAA TAACTTCCTG ATGACAGGAC CAAAGGCTTA TCTCGCATAC 
ACCAGCAGTG TACAAGCTGG GGCTCAGAGT GGTATAGAAG AGTGCAAGCA TCAGTTCGCA 
TGGGACAGGT GGAACTGCCC GGAAAGCGCA CTGCAGTTAT CGACGCATAA AGGCCTGCGG 
AGTGCCACCA GAGAGACCGC CTTTGTGCAT GCTATAAGTG CTGCTGGAGT TATGTACACT 
TTGACGAAAA ACTGCAGCAT GGGGGACTTC GAAAACTGTG GCTGTGACGA CTCCAAGATC 
GGAAAAATGG GTGGTCGTGG TTGGGTTTGG GGAGGTTGCA GTGACAATGT TAACTTTGGA 
GACAGAATTG CTAAACTATT TGTAGACGCG CTGGAAAATG GTCACGACTC TCGCGCCGCG 
GTCAACCTGC ATAACAACGA AGCTGGTCGA CTTGCTGTTA AAGCAACACT CAAAAGGACC 
TGTAAGTGTC ATGGTTTGTC TGGAAGCTGT AGTATTCAGA CATGCTGGAT GCAGCTTGCT 
GATTTCAGAG ATATCGGCTC CTACCTAAAA ATCAAGCATG ATCAAGCACG GAAGTTGGAG 
ATGGATAAAA TTCGGATGAG GGCAGGCAAT AGCGCGGACA ACCGTGGCGC AATAGCGGAC 
ACGTTCAGCG CAGTTGCGCG CACAGAACTC ATTTTTATGG AAGATTCACC TGATTACTGC 
GTGAAAAACC TCAGCATGGG ACTACATGGA ACTGAAGGCA GGGAATGTCT ACAAAGCGGG 
AAGAATCTTT CTCAATGGGA GAGAAGAAGC TGCAGGCGGC TATGCCACGA ATGTGGCCTG 
AAAGTAGAAG AGAGGAGGAT TGAGACAGTG AGCAGCTGTA ACTGTAAATT TCACTGGTGC 
TGCACAGTCA AATGCGAAAC ATGCACTCAA ACCGTTACCA GGTATTTTTG CGCAAAAAGG 
CACAGAAATC GCCGGCCGCA CAACCATTCA CGCAAAAGAC AACACACGCG TCGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571021.3
CDS	164..1243
Translation	MNPCQIFASLVMSICCHILSSTAWSVNNFLMTGPKAYLAYTSSVQAGAQSGIEECKHQFAWDRWNCPESALQLSTHKGLRSATRETAFVHAISAAGVMYTLTKNCSMGDFENCGCDDSKIGKMGGRGWVWGGCSDNVNFGDRIAKLFVDALENGHDSRAAVNLHNNEAGRLAVKATLKRTCKCHGLSGSCSIQTCWMQLADFRDIGSYLKIKHDQARKLEMDKIRMRAGNSADNRGAIADTFSAVARTELIFMEDSPDYCVKNLSMGLHGTEGRECLQSGKNLSQWERRSCRRLCHECGLKVEERRIETVSSCNCKFHWCCTVKCETCTQTVTRYFCAKRHRNRRPHNHSRKRQHTRRG

Target ORF information:

RefSeq Version	NM_130946.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wingless-type MMTV integration site family, member 8a (wnt8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130946.3

ORF Insert Sequence:

ATGAACCCTT GCCAGATTTT TGCGTCGTTG GTTATGTCTA TATGCTGTCA CATACTGTCG 
TCAACAGCAT GGTCTGTGAA TAACTTCCTG ATGACAGGAC CAAAGGCTTA TCTCGCATAC 
ACCAGCAGTG TACAAGCTGG GGCTCAGAGT GGTATAGAAG AGTGCAAGCA TCAGTTCGCA 
TGGGACAGGT GGAACTGCCC GGAAAGCGCA CTGCAGTTAT CGACGCATAA AGGCCTGCGG 
AGTGCCACCA GAGAGACCGC CTTTGTGCAT GCTATAAGTG CTGCTGGAGT TATGTACACT 
TTGACGAAAA ACTGCAGCAT GGGGGACTTC GAAAACTGTG GCTGTGACGA CTCCAAGATC 
GGAAAAATGG GTGGTCGTGG TTGGGTTTGG GGAGGTTGCA GTGACAATGT TAACTTTGGA 
GACAGAATTG CTAAACTATT TGTAGACGCG CTGGAAAATG GTCACGACTC TCGCGCCGCG 
GTCAACCTGC ATAACAACGA AGCTGGTCGA CTTGCTGTTA AAGCAACACT CAAAAGGACC 
TGTAAGTGTC ATGGTTTGTC TGGAAGCTGT AGTATTCAGA CATGCTGGAT GCAGCTTGCT 
GATTTCAGAG ATATCGGCTC CTACCTAAAA ATCAAGCATG ATCAAGCACG GAAGTTGGAG 
ATGGATAAAA TTCGGATGAG GGCAGGCAAT AGCGCGGACA ACCGTGGCGC AATAGCGGAC 
ACGTTCAGCG CAGTTGCGCG CACAGAACTC ATTTTTATGG AAGATTCACC TGATTACTGC 
GTGAAAAACC TCAGCATGGG ACTACATGGA ACTGAAGGCA GGGAATGTCT ACAAAGCGGG 
AAGAATCTTT CTCAATGGGA GAGAAGAAGC TGCAGGCGGC TATGCCACGA ATGTGGCCTG 
AAAGTAGAAG AGAGGAGGAT TGAGACAGTG AGCAGCTGTA ACTGTAAATT TCACTGGTGC 
TGCACAGTCA AATGCGAAAC ATGCACTCAA ACCGTTACCA GGTATTTTTG CGCAAAAAGG 
CACAGAAATC GCCGGCCGCA CAACCATTCA CGCAAAAGAC AACACACGCG TCGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of maternal wnt8a transcripts in axis formation in zebrafish.
Developmental biology(2017 Dec)
Hino H,Nakanishi A,Seki R,Aoki T,Yamaha E,Kawahara A,Shimizu T,Hibi M

Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments.
The FEBS journal284(15)2513-2526(2017 Aug)
Sezgin E,Azbazdar Y,Ng XW,Teh C,Simons K,Weidinger G,Wohland T,Eggeling C,Ozhan G

Harmless effects of argon plasma on caudal fin regeneration and embryogenesis of zebrafish: novel biological approaches for safe medical applications of bioplasma.
Experimental & molecular medicine49(7)e355(2017 Jul)
Nam MK,Kim GY,Yun SE,Jang JY,Kim YH,Choi EH,Kang S,Rhim H

Wnt8a expands the pool of embryonic kidney progenitors in zebrafish.
Developmental biology425(2)130-141(2017 05)
Naylor RW,Han HI,Hukriede NA,Davidson AJ

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wnt8a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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