skib cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	skib
Entrez Gene ID	30113
Full Name	v-ski avian sarcoma viral oncogene homolog b
Synonyms	fc17g02,wu:fc17g02,wu:fk79g04
General protein information	Preferred Names v-ski avian sarcoma viral oncogene homolog b Names v-ski avian sarcoma viral oncogene homolog b nuclear oncoprotein skib
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130938.1	NP_571013.1	v-ski avian sarcoma viral oncogene homolog b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in the TGF-? repressor SKI cause Shprintzen-Goldberg syndrome with aortic aneurysm.
Doyle AJ, Doyle JJ, Bessling SL, Maragh S, Lindsay ME, Schepers D, Gillis E, Mortier G, Homfray T, Sauls K, Norris RA, Huso ND, Leahy D, Mohr DW, Caulfield MJ, Scott AF, Destr?e A, Hennekam RC, Arn PH, Curry CJ, Van Laer L, McCallion AS, Loeys BL, Dietz HC
Nature genetics44(11)1249-54(2012 Nov)

Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish.
Asakawa K, Suster ML, Mizusawa K, Nagayoshi S, Kotani T, Urasaki A, Kishimoto Y, Hibi M, Kawakami K
Proceedings of the National Academy of Sciences of the United States of America105(4)1255-60(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following skib gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the skib cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03506	NM_130938.1 Latest version!	Danio rerio v-ski avian sarcoma viral oncogene homolog b (skib), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

skib ( NM_130938.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571013.1 Danio rerio v-ski avian sarcoma viral oncogene homolog b (skib), mRNA.

Price & Availability↑

CloneID	ODa03506
Clone ID Related Accession (Same CDS sequence)	NM_130938.1
Accession Version	NM_130938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	v-ski avian sarcoma viral oncogene homolog b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF060118.1. ##Evidence-Data-START## Transcript exon combination :: AF060118.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGCTC CTTCCAGCTT TCAGCCGCAT CCAGGACTTC AGCAAACTCT CAAGCAGTTT 
CACTTGAGCT CCATGAGCTC CCTCGGCGGA CCGGCAGCCT TCTCGGCCCG GTGGCAGCAG 
GACATGCTGT TCAAAAAGGA CGGTAAGAGC GCCGAGGTGC TCGTGAACCT CCCCGCGCAG 
ACGCCCCCGG TCATGCCGGG ACCCCTGTTC ATCCCGTCCG ACCGCTCCAC CGAGAGGTGC 
GAGACGGTCC TGGAGCGGGA GACTATTTCG TGCTTCGTGG TGGGCGGCGA GAAGCGCCTG 
TGCCTGCCGC AGATCCTCAA TAGCGTCCTG CGAGACTTTT CCCTCCAGCA GATCAACTCT 
GTGTGCGACG ACCTGCACAT CTACTGCTCC AGGTGCACGG CGGACCAGCT GGAGATCCTC 
AAAGTTATGG GCATCCTGCC CTTCTCGGCC CCCTCGTGCG GACTCATCAC ACAAACAGAC 
GCCGAGCGCC TCTGCAATGC CCTCATCTAC GGCGGCACGT TCCCCCCGCA CGCCGACAAA 
GAGTTGTCCT GCTCCATTGA GCTGGAGCGC ACAGAGAAGA GCTTCAAAGT TTACCACGAA 
TGCTTTGGGA AGTGCAAGGG CCTGTTCGTA CCCGAACTCT ACACCAGCCC CAACGCGGCG 
TGCATACAGT GCTTGGACTG CAGGCTCATG TTCCCGACCC ACAAGTTCGT GGTGCACAGT 
CATAAAAGGC TGGAGAACAG AACCTGTCAC TGGGGCTTCG ACTCGTCCAA CTGGAGGGCT 
TATATTCTTC TAGATCAAGA CTATAGCGAG AAAGAGGAGA ACAGCACACT GCAGCAGCTC 
CTCACTGAGT TAAAGGGAAA ATTTGAAATG GGGAACAAGC ACAAGACATC TTACAAGGCA 
TACAGTCCTA TTCCCTCCAA AAAGCTTAAA CATGATCGCT CCAGATCGCC ATCAGCAGAT 
AGAGAGAAGC CAGCTGATTG GCTACAGTCT CTGGCGGCTT CTGCTAACAA GGATCTTAAG 
CAGCTCCAGT TCAAGCAGAG ACCTTCAGCC TTCAGGCCCT GGTCTCCCAG AGTTCCCTCT 
GCTGAGCGAG AGACTCCCAA TCACAAGTAT GAGAGATCGC TCGCTAAAAC GCAAGACAAT 
CTGGCAGTTC CAAATGTGTC TCTGCCCATT GCTGCCTTCA AAAATAATGA GCAGCCAGCA 
CACCGTTTCT CTCAGCCCAC TGAGAGCCAC AACACAGGCA AGACGGAGAG CTCCAGGCCA 
AGAAAGAGAT GGCCGTCAGC AGAGAGACAA TCACAGCCAG CCACCAAAAC CTCTCTGTGC 
ACTCCAATCA AACAGCGGCT GCAGGAGAAC CACGACTCAG ATGAGGAAAT CCAGGTGGAC 
AACTGTGCTG AAGTTGCTGT CTCTCGTTCG CTGATGTCTC CTCCCTTCAC CAATGGTGTC 
GGGGTGATGA CCCTCAGCCC TCACAGCTCT GCAGAGGTGC ATGATGGGAG GGCGAGACTG 
GCAGCGGGTC ATTGTTCAGA GCTGGAGCTC CTCAAGCAGA CGCTCAGCAG TGACCTGAGC 
TCCAAAGAGG CCAGAGAGAA GTTCCTGCAG GAGATCATCC GCATGAGGGT GAAGCAGGAG 
GAGAAACTGG CAGCCGCTCT GCAAGCCAAA CGCAGCCTGC AGCAGGAGCT GGAGTTTGTC 
AGGGTGACTA AGAAGGGCCG GCTGAGGGAG GCCATCGAAG CCAAGCGGAA CCTAAGGAAG 
GAGATCGAGC GCCTCCGTGT GGAGTGTGAG AAGAAGGTGC GGGACGCCAG CGAGTCCTGC 
GACAGGCTGA AGAGGGATTT GGACAGAGAG AGACGGACCC GTGTCTGTGA CAAGAACTGT 
GAAGCAGGAC GTCTGAGGGC CAAATACTCC TCACAGATTG AGGATCTGCA GATGAAGCTC 
CAGCAGGCAG AAGTGGACCG TGAACAGCTG AGAGAAGACC TGCTGAAGGA ACGAGACGCC 
CGACAAAACC TGGAGCGGGT CGTAAAAGAG CTTCAGGAGC AACTGGGCCA CAAAGCAGAA 
ACGGAGGCTA ACACACAGCC CACCAAAGGA GATCAAGACA TTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571013.1
CDS	337..2424
Translation	MEAPSSFQPHPGLQQTLKQFHLSSMSSLGGPAAFSARWQQDMLFKKDGKSAEVLVNLPAQTPPVMPGPLFIPSDRSTERCETVLERETISCFVVGGEKRLCLPQILNSVLRDFSLQQINSVCDDLHIYCSRCTADQLEILKVMGILPFSAPSCGLITQTDAERLCNALIYGGTFPPHADKELSCSIELERTEKSFKVYHECFGKCKGLFVPELYTSPNAACIQCLDCRLMFPTHKFVVHSHKRLENRTCHWGFDSSNWRAYILLDQDYSEKEENSTLQQLLTELKGKFEMGNKHKTSYKAYSPIPSKKLKHDRSRSPSADREKPADWLQSLAASANKDLKQLQFKQRPSAFRPWSPRVPSAERETPNHKYERSLAKTQDNLAVPNVSLPIAAFKNNEQPAHRFSQPTESHNTGKTESSRPRKRWPSAERQSQPATKTSLCTPIKQRLQENHDSDEEIQVDNCAEVAVSRSLMSPPFTNGVGVMTLSPHSSAEVHDGRARLAAGHCSELELLKQTLSSDLSSKEAREKFLQEIIRMRVKQEEKLAAALQAKRSLQQELEFVRVTKKGRLREAIEAKRNLRKEIERLRVECEKKVRDASESCDRLKRDLDRERRTRVCDKNCEAGRLRAKYSSQIEDLQMKLQQAEVDREQLREDLLKERDARQNLERVVKELQEQLGHKAETEANTQPTKGDQDIS

Target ORF information:

RefSeq Version	NM_130938.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-ski avian sarcoma viral oncogene homolog b (skib), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130938.1

ORF Insert Sequence:

ATGGAGGCTC CTTCCAGCTT TCAGCCGCAT CCAGGACTTC AGCAAACTCT CAAGCAGTTT 
CACTTGAGCT CCATGAGCTC CCTCGGCGGA CCGGCAGCCT TCTCGGCCCG GTGGCAGCAG 
GACATGCTGT TCAAAAAGGA CGGTAAGAGC GCCGAGGTGC TCGTGAACCT CCCCGCGCAG 
ACGCCCCCGG TCATGCCGGG ACCCCTGTTC ATCCCGTCCG ACCGCTCCAC CGAGAGGTGC 
GAGACGGTCC TGGAGCGGGA GACTATTTCG TGCTTCGTGG TGGGCGGCGA GAAGCGCCTG 
TGCCTGCCGC AGATCCTCAA TAGCGTCCTG CGAGACTTTT CCCTCCAGCA GATCAACTCT 
GTGTGCGACG ACCTGCACAT CTACTGCTCC AGGTGCACGG CGGACCAGCT GGAGATCCTC 
AAAGTTATGG GCATCCTGCC CTTCTCGGCC CCCTCGTGCG GACTCATCAC ACAAACAGAC 
GCCGAGCGCC TCTGCAATGC CCTCATCTAC GGCGGCACGT TCCCCCCGCA CGCCGACAAA 
GAGTTGTCCT GCTCCATTGA GCTGGAGCGC ACAGAGAAGA GCTTCAAAGT TTACCACGAA 
TGCTTTGGGA AGTGCAAGGG CCTGTTCGTA CCCGAACTCT ACACCAGCCC CAACGCGGCG 
TGCATACAGT GCTTGGACTG CAGGCTCATG TTCCCGACCC ACAAGTTCGT GGTGCACAGT 
CATAAAAGGC TGGAGAACAG AACCTGTCAC TGGGGCTTCG ACTCGTCCAA CTGGAGGGCT 
TATATTCTTC TAGATCAAGA CTATAGCGAG AAAGAGGAGA ACAGCACACT GCAGCAGCTC 
CTCACTGAGT TAAAGGGAAA ATTTGAAATG GGGAACAAGC ACAAGACATC TTACAAGGCA 
TACAGTCCTA TTCCCTCCAA AAAGCTTAAA CATGATCGCT CCAGATCGCC ATCAGCAGAT 
AGAGAGAAGC CAGCTGATTG GCTACAGTCT CTGGCGGCTT CTGCTAACAA GGATCTTAAG 
CAGCTCCAGT TCAAGCAGAG ACCTTCAGCC TTCAGGCCCT GGTCTCCCAG AGTTCCCTCT 
GCTGAGCGAG AGACTCCCAA TCACAAGTAT GAGAGATCGC TCGCTAAAAC GCAAGACAAT 
CTGGCAGTTC CAAATGTGTC TCTGCCCATT GCTGCCTTCA AAAATAATGA GCAGCCAGCA 
CACCGTTTCT CTCAGCCCAC TGAGAGCCAC AACACAGGCA AGACGGAGAG CTCCAGGCCA 
AGAAAGAGAT GGCCGTCAGC AGAGAGACAA TCACAGCCAG CCACCAAAAC CTCTCTGTGC 
ACTCCAATCA AACAGCGGCT GCAGGAGAAC CACGACTCAG ATGAGGAAAT CCAGGTGGAC 
AACTGTGCTG AAGTTGCTGT CTCTCGTTCG CTGATGTCTC CTCCCTTCAC CAATGGTGTC 
GGGGTGATGA CCCTCAGCCC TCACAGCTCT GCAGAGGTGC ATGATGGGAG GGCGAGACTG 
GCAGCGGGTC ATTGTTCAGA GCTGGAGCTC CTCAAGCAGA CGCTCAGCAG TGACCTGAGC 
TCCAAAGAGG CCAGAGAGAA GTTCCTGCAG GAGATCATCC GCATGAGGGT GAAGCAGGAG 
GAGAAACTGG CAGCCGCTCT GCAAGCCAAA CGCAGCCTGC AGCAGGAGCT GGAGTTTGTC 
AGGGTGACTA AGAAGGGCCG GCTGAGGGAG GCCATCGAAG CCAAGCGGAA CCTAAGGAAG 
GAGATCGAGC GCCTCCGTGT GGAGTGTGAG AAGAAGGTGC GGGACGCCAG CGAGTCCTGC 
GACAGGCTGA AGAGGGATTT GGACAGAGAG AGACGGACCC GTGTCTGTGA CAAGAACTGT 
GAAGCAGGAC GTCTGAGGGC CAAATACTCC TCACAGATTG AGGATCTGCA GATGAAGCTC 
CAGCAGGCAG AAGTGGACCG TGAACAGCTG AGAGAAGACC TGCTGAAGGA ACGAGACGCC 
CGACAAAACC TGGAGCGGGT CGTAAAAGAG CTTCAGGAGC AACTGGGCCA CAAAGCAGAA 
ACGGAGGCTA ACACACAGCC CACCAAAGGA GATCAAGACA TTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in the TGF-? repressor SKI cause Shprintzen-Goldberg syndrome with aortic aneurysm.
Nature genetics44(11)1249-54(2012 Nov)
Doyle AJ,Doyle JJ,Bessling SL,Maragh S,Lindsay ME,Schepers D,Gillis E,Mortier G,Homfray T,Sauls K,Norris RA,Huso ND,Leahy D,Mohr DW,Caulfield MJ,Scott AF,Destr?e A,Hennekam RC,Arn PH,Curry CJ,Van Laer L,McCallion AS,Loeys BL,Dietz HC

Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish.
Proceedings of the National Academy of Sciences of the United States of America105(4)1255-60(2008 Jan)
Asakawa K,Suster ML,Mizusawa K,Nagayoshi S,Kotani T,Urasaki A,Kishimoto Y,Hibi M,Kawakami K

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skib cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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