nme2b.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nme2b.1
Entrez Gene ID	30083
Full Name	NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1
Synonyms	NDPK-Z1,NM23-B,NM23-Z1,ndpkz1,nm23b,nme1,nme2,nme2b1
General protein information	Preferred Names NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 Names nucleoside diphosphate kinase A non-metastatic cells 2, protein (NM23B) expressed in non-metastatic cells 2b.1, protein (NM23B) expressed in nucleoside diphosphate kinase-Z1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130926.1	NP_571001.1	nucleoside diphosphate kinase A
NM_001362266.1	NP_001349195.1	nucleoside diphosphate kinase A isoform c
XM_017352177.1	XP_017207666.1	nucleoside diphosphate kinase A isoform X1
NM_001362264.1	NP_001349193.1	nucleoside diphosphate kinase A isoform a
NM_001362265.1	NP_001349194.1	nucleoside diphosphate kinase A isoform b
XM_017352177.2	XP_017207666.1	nucleoside diphosphate kinase A isoform X1
NM_130926.2	NP_571001.2	nucleoside diphosphate kinase A isoform b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nme1	NP_612557.1
Pan troglodytes (chimpanzee)	NME1	NP_001191440.1
Canis lupus familiaris (dog)	NME1	NP_001019808.1
Arabidopsis thaliana (thale cress)	NDPK3	NP_192839.1
Homo sapiens (human)	NME1	NP_937818.1
Bos taurus (cattle)	NME1	NP_991387.2
Gallus gallus (chicken)	NME1	NP_001191690.1
Danio rerio (zebrafish)	nme2b.1	NP_571001.1
Arabidopsis thaliana (thale cress)	AT4G23895	NP_001190817.1
Oryza sativa (rice)	Os05g0595400	NP_001056515.1
Mus musculus (house mouse)	Nme1	NP_032730.1
Arabidopsis thaliana (thale cress)	AT4G23900	NP_567690.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition.
Du Z, Chen X, Li X, He K, Ji S, Shi W, Hao A
Biochemical and biophysical research communications475(2)194-201(2016 06)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Stem Cell Differentiation Stage Factors from Zebrafish Embryo: A Novel Strategy to Modulate the Fate of Normal and Pathological Human (Stem) Cells.
Biava PM, Canaider S, Facchin F, Bianconi E, Ljungberg L, Rotilio D, Burigana F, Ventura C
Current pharmaceutical biotechnology16(9)782-92(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NDPKB is required for VEGF-induced angiogenesis and contributes to the correct localization of VEGF receptor type 2 and VE-cadherin at the endothelial adherens junctions.
Title: Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.

Data show that NDPK B knockdown embryo results in a severe decrease in cardiac contractility.
Title: The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function.

The following nme2b.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nme2b.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37828	NM_130926.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa159688	NM_001362265.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa159688	NM_130926.2 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa159690	NM_001362266.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa56024	NM_001362264.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa56024	XM_017352177.2 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
ODa56024	XM_017352177.1	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nme2b.1 ( NM_130926.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571001.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), mRNA.

Price & Availability↑

CloneID	ODa37828
Clone ID Related Accession (Same CDS sequence)	NM_130926.1
Accession Version	NM_130926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF201764.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF201764.1, CK355005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCTA AGACCGAGCG CACCTTTATC GCGGCGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571001.1
CDS	40..501
Translation	MSAKTERTFIAAKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	NM_130926.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130926.1

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTTATC GCGGCGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( NM_001362265.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349194.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ODa159688
Clone ID Related Accession (Same CDS sequence)	NM_001362265.1 , NM_130926.2
Accession Version	NM_001362265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694252.9. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CT586938.2, CT703956.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349194.1
CDS	96..557
Translation	MSAKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	NM_001362265.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362265.1

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( NM_130926.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571001.2 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa159688
Clone ID Related Accession (Same CDS sequence)	NM_001362265.1 , NM_130926.2
Accession Version	NM_130926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694252.9. On Apr 11, 2018 this sequence version replaced NM_130926.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CO355876.1, EH443774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571001.2
CDS	90..551
Translation	MSAKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	NM_130926.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130926.2

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG CCTCTGAGGA TCTCCTGAAG CAGCACTACA TTGACCTGAA GGACCGGCCA 
TTTTACCCCG GACTGGTGAA GTACATGAGC TCTGGACCTG TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGGTGAAGAC CGGGCGAGTG ATGCTCGGAG AGACCAACCC TGCAGACTCC 
AAACCCGGAA CCATCAGGGG AGACTTCTGC ATTGAAGTGG GCAGGAACAT CATCCACGGC 
AGTGACTCTG TGGACAGCGC TAACACTGAG ATCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTTCA AGAGCTGCGC ACAGCAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( NM_001362266.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349195.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ODa159690
Clone ID Related Accession (Same CDS sequence)	NM_001362266.1
Accession Version	NM_001362266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694252.9. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EV603286.1, CT636841.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCTG GACCTGTTCT GGCCATGGTC TGGGAGGGTC TGAATGTGGT GAAGACCGGG 
CGAGTGATGC TCGGAGAGAC CAACCCTGCA GACTCCAAAC CCGGAACCAT CAGGGGAGAC 
TTCTGCATTG AAGTGGGCAG GAACATCATC CACGGCAGTG ACTCTGTGGA CAGCGCTAAC 
ACTGAGATCA GCCTGTGGTT CAAGCCGGAG GAGCTGGTGT CCTTCAAGAG CTGCGCACAG 
CAGTGGATCT ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349195.1
CDS	161..418
Translation	MSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	NM_001362266.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362266.1

ORF Insert Sequence:

ATGAGCTCTG GACCTGTTCT GGCCATGGTC TGGGAGGGTC TGAATGTGGT GAAGACCGGG 
CGAGTGATGC TCGGAGAGAC CAACCCTGCA GACTCCAAAC CCGGAACCAT CAGGGGAGAC 
TTCTGCATTG AAGTGGGCAG GAACATCATC CACGGCAGTG ACTCTGTGGA CAGCGCTAAC 
ACTGAGATCA GCCTGTGGTT CAAGCCGGAG GAGCTGGTGT CCTTCAAGAG CTGCGCACAG 
CAGTGGATCT ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( NM_001362264.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349193.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa56024
Clone ID Related Accession (Same CDS sequence)	XM_017352177.1 , NM_001362264.1 , XM_017352177.2
Accession Version	NM_001362264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694252.9 and EH513618.1. On Apr 11, 2018 this sequence version replaced XM_017352177.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EH487224.1, EH512654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349193.1
CDS	90..584
Translation	MSAKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQVCVLMVDQDAVASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	NM_001362264.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362264.1

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( XM_017352177.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207666.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.

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CloneID	ODa56024
Clone ID Related Accession (Same CDS sequence)	XM_017352177.1 , NM_001362264.1 , XM_017352177.2
Accession Version	XM_017352177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleoside diphosphate kinase A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207666.1
CDS	111..605
Translation	MSAKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQVCVLMVDQDAVASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	XM_017352177.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352177.1

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.1 ( XM_017352177.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207666.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56024
Clone ID Related Accession (Same CDS sequence)	XM_017352177.1 , NM_001362264.1 , XM_017352177.2
Accession Version	XM_017352177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207666.1
CDS	119..613
Translation	MSAKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAMKFLQVCVLMVDQDAVASEDLLKQHYIDLKDRPFYPGLVKYMSSGPVLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIEVGRNIIHGSDSVDSANTEISLWFKPEELVSFKSCAQQWIYE

Target ORF information:

RefSeq Version	XM_017352177.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 (nme2b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352177.2

ORF Insert Sequence:

ATGTCTGCTA AGACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCATGAAG 
TTTCTGCAGG TGTGTGTGCT GATGGTGGAT CAGGATGCAG TGGCCTCTGA GGATCTCCTG 
AAGCAGCACT ACATTGACCT GAAGGACCGG CCATTTTACC CCGGACTGGT GAAGTACATG 
AGCTCTGGAC CTGTTCTGGC CATGGTCTGG GAGGGTCTGA ATGTGGTGAA GACCGGGCGA 
GTGATGCTCG GAGAGACCAA CCCTGCAGAC TCCAAACCCG GAACCATCAG GGGAGACTTC 
TGCATTGAAG TGGGCAGGAA CATCATCCAC GGCAGTGACT CTGTGGACAG CGCTAACACT 
GAGATCAGCC TGTGGTTCAA GCCGGAGGAG CTGGTGTCCT TCAAGAGCTG CGCACAGCAG 
TGGATCTACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition.
Biochemical and biophysical research communications475(2)194-201(2016 06)
Du Z,Chen X,Li X,He K,Ji S,Shi W,Hao A

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Stem Cell Differentiation Stage Factors from Zebrafish Embryo: A Novel Strategy to Modulate the Fate of Normal and Pathological Human (Stem) Cells.
Current pharmaceutical biotechnology16(9)782-92(2015)
Biava PM,Canaider S,Facchin F,Bianconi E,Ljungberg L,Rotilio D,Burigana F,Ventura C

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nme2b.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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