lamc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lamc1
Entrez Gene ID	286832
Full Name	laminin, gamma 1
Synonyms	sly
General protein information	Preferred Names laminin, gamma 1 Names laminin subunit gamma-1 shortstop shp sleepy
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173277.1	NP_775384.1	laminin subunit gamma-1 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lamc1	NP_775384.1
Xenopus tropicalis (tropical clawed frog)	lamc1	NP_001090659.1
Homo sapiens (human)	LAMC1	NP_002284.3
Canis lupus familiaris (dog)	LAMC1	XP_537156.3
Rattus norvegicus (Norway rat)	Lamc1	NP_446418.1
Anopheles gambiae (African malaria mosquito)	LANB2	XP_308240.3
Pan troglodytes (chimpanzee)	LAMC1	XP_001162648.1
Bos taurus (cattle)	LAMC1	NP_001193746.1
Caenorhabditis elegans (roundworm)	lam-2	NP_509204.3
	LOC715265	XP_002808304.1
Mus musculus (house mouse)	Lamc1	NP_034813.2
Gallus gallus (chicken)	LAMC1	XP_001234659.2
Drosophila melanogaster (fruit fly)	LanB2	NP_524006.1

Gene Ontology
Bibliography

Related articles in PubMed

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Chen WC, Wang Z, Missinato MA, Park DW, Long DW, Liu HJ, Zeng X, Yates NA, Kim K, Wang Y
Science advances2(11)e1600844(2016 Nov)

Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
Jenkins MH, Alrowaished SS, Goody MF, Crawford BD, Henry CA
Skeletal muscle618(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hh signaling is indirectly required via slow fiber specification for recovery of fast fiber elongation in laminin gamma1 mutant embryos
Title: Hedgehog signaling and laminin play unique and synergistic roles in muscle development.

Two zebrafish loci, grumpy and sleepy, encode laminin beta1 and laminin gamma1, respectively. These genes are required for the formation of the laminin-dependent basement membrane is essential for the differentiation of chordamesoderm to notochord.
Title: Zebrafish mutants identify an essential role for laminins in notochord formation.

The following lamc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lamc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01454	NM_173277.1 Latest version!	Danio rerio laminin, gamma 1 (lamc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lamc1 ( NM_173277.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775384.1 Danio rerio laminin, gamma 1 (lamc1), mRNA.

Price & Availability↑

CloneID	ODa01454
Clone ID Related Accession (Same CDS sequence)	NM_173277.1
Accession Version	NM_173277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Danio rerio(zebrafish)
Product	laminin subunit gamma-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF468048.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF468048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTTT TTAGCTGTTT GTTGTTATGG ACTTTGTGGG CAGCGTGCAG TCACGGAGCG 
ATGGATGAAT GTATAGACGA GGATGATCGG CCTCAGCGCT GCATGCCGGA GTTCGTTAAC 
GCGGCTTTCA ATGCGACCGT GGTGGCCACC AACACTTGCG GCTCGCCGCC TGAAGAGTTC 
TGCGTACAGA CCGGGGTTAC GGGAGTCACT AAATCATGCC ACATTTGCAA TGCCGCGGAC 
CCCAGACTGC ACCACGGCGC TGTTTATCTC ACCGATTACA ACCAACCAGT ACAACCAACC 
TGGTGGCAAA GCCAGACTAT GTTAGCGGGC ATCCAGTACC CGAACTCCAT CAACTTGACC 
CTTCACCTGG GCAAATCTTT CGACATCACC TATGTGCGTT TGAAGTTCCA CACCAGCCGT 
CCTGAGAGTT TTGCCATCTA CAAGCGCTCC AGTGAAGACG GGCCTTGGAC CCCCTACCAA 
TACTACAGTG GCTCCTGTGA GAAAACCTAC AGCAAAAACA ACCGTGGCTT CATCCGCACG 
GGCGAGGACG AGCAGCAGGC TCTGTGTACA GATGAGTTTA GCGACATCTC GCCACTCTAT 
GGAGGAAACG TGGCTTTCTC TACGCTCGAG GGCCGACCCA GTGCATATAA CTTCGACAAC 
AGCCCTGTAC TGCAGGATTG GGTAACTGCC ACTGATATCA GAGTGACCCT GAATCGATTA 
AACACTTTCG GAGATGAAGT TTTCAACGAT CCCAAAGTCC TCAAATCCTA CTACTACGCC 
ATCTCGGATT TTGCTGTGGG AGGAAGGTGC AAGTGCAATG GCCATGCCAG CGAGTGCGTG 
AAGAACGAGT ACAGCAAGCT GGTGTGTAAC TGCAAACACA ACACAGAAGG AGCCGACTGC 
AATGTGTGCA AACCCTTCTA TAATGACCGT CCCTGGAGGA GAGCCACTGC TGAAAACCCT 
AATGAGTGTC TGCCTTGTAA CTGTAATGGA AAGAGCGCTG AATGTTACTT CGACCCTGAG 
CTGTATCGTG CCACTGGTCA CGGAGGTCAC TGCCGTAACT GTGCTGACAA TACAGATGGG 
CCGAAGTGTG AGCGATGCCT GGCCAACTAC TACAGAGAGG CCTCCGGTCA GCGCTGTCTG 
TCCTGCGGCT GCAACCCTGT TGGGTCTCTC AGCACTCAGT GTGATAACAC TGGCAGATGC 
AGCTGTAAGC CAGGAGTGAT GGGAGACAAG TGTGACAGAT GCCAACCTGG ATACCACTCG 
CTCTCTGAGG CAGGATGCAG ACCGTGCTCT TGTAATCCAG CCGGAAGCAC ACAGGAGTGT 
GATGTCCAGA CCGGACGCTG CCAGTGCAAG GAAAATGTGG ACGGATTCAA CTGTGACCGG 
TGTAAACTGG GCTATTTTAA TCTGGACCCC CAGAATCCTC AGGGCTGCAC TCCATGCTTC 
TGCTTCCAGC ATTCGACAGT GTGTGAGAGC GCAGACGGCT ACAGTGTGCA CAAAATCACC 
TCCACCTTTG ACAGAGATGA TGAAGGCTGG AAGGGTAAAC AGAGAGATGA CTCCAGTGTA 
CCGGTACAGT GGTCACCCAG TTCTGGAGAG ATCTCACTCA TCTCAGAAGA TTACTTCCCC 
ATCTACTTTG TGGCTCCAGA TAAATTTCTG CATAATCAGC TGTTGAGTTA TGGTCAGAAC 
CTCACGCTGA ACTTCAGGAT TCAGCGTCAC GATGCTCGCC TCTCAGCAGA GGACGTTGTG 
CTGGAGGGCT CGGGGCTTCG TGTCGCTGTG CCTCTTATTG CTCAGGGTAA TTCCTACCCA 
GGAGAGGAGA CCCAAACCTT TGTGTTCAGA CTACATGACA CAACTGACTA CCCTTGGAGA 
CCCACCATAA AGCATGCAGA CTTCCAGAAA CTTCTCTACA ATCTGACTTC AATCATGATC 
CGTGGAACCT ACAGCGCACA AAGTGCTGGT TATTTGGATA ACGTGTCTCT GGTGACTGCC 
CGCCGGGGCC CTGGCACTCC GGCCCGATGG GTTGAGAAAT GCACGTGTCC GCAGGGTTAT 
TTGGGTCAGC ATTGTGAGCA GTGCGATCAG GGATTCAGGA GGAGCCGGCC AGAGCTGCGA 
CGCTTCAGCA CCTGCGAGCG CTGCAACTGC AATGGACACA GCGACACATG TGACCCTGAG 
ACAGGAATGT GTAACTGCCA GCACAACACT GCTGGTTTGA GCTGTGAAAG GTGTAAAGAC 
GGCTTCTATG GTGATTCTAC AGTGGGCTCT TCTTCAGACT GTAAAGCCTG TCCCTGCCCT 
GCTGGTGCCA CCTGTGCTGT GGTGCCCAAG ACCAATGAGG TGGTCTGTAC CAACTGCCCG 
ACTGGAACCA CAGGTAAACG ATGTGAGCTG TGTGACGATG GTTTCTTTGG AGATCCTCTG 
GGTGAGAAAG GTCCAGTGAG AGCGTGTCGA GCCTGCAGCT GCAACAACAA CATCGAGCCG 
AACGCTGTGG GCAACTGTAA CCGAGAGTCG GGCGAATGCC TCAAGTGCAT CTACAACACC 
GCTGGCGTCT TTTGCGACCG CTGCAAGCAG GGCTTCTATG GTGATGCCCG CGCTGCTAAT 
GTAGCCGACA AATGCAAACC GTGCAAGTGT TCTCCATATG GTACAGTAGA CCGACAGACA 
GCCTGTTCTC AGGTGACGGG TCAATGTCCG TGTCTCCCTC ACGTCATTAA CAGAGACTGT 
GGCGCTTGTG AACTGGGTTT CTACAATCTG CAGAGTGGAA AGGGCTGTGA ACGATGCAAC 
TGCAACCCTA TTGGTTCCAC GAATGGTCAG TGTGACATTG TCAGCGGTCA GTGTGAGTGT 
CAGCCTGGAG TCACCGGTCA ACATTGTGAG CGATGCGAGG TCAACTTCTT CGGATTCAGT 
TCATCGGGTT GCAAACCATG TGACTGTGAC CCAGAAGGCT CAGAATCAGC TCAGTGTAAG 
GAGGACGGCC GCTGTCATTG TCGGCCAGGT TTTGTGGGCT CTCGCTGTGA TATGTGTGAG 
GAAAACTACT TCTACAACCG CTCGACACCT GGCTGCCAGC AGTGCCCTAA CTGTTACAGC 
CTGGTCAGAG ACAAGGTAAA CCAGCAGAGA CAAAAACTGC TTGACCTGCA GAATTTGATT 
GATAGTCTGG ACAACACTGA GACGACAGTG AGCGACAAGG CCTTTGAGGA CAGACTGAAG 
GAGGCAGAGA AAACTATAAT GGACTTGCTG GAAGAGGCTC AGGCTAGCAA AGAAGTGGAT 
AAAGGTCTTT TGGACCGTCT AAACAACATC AACAAGACCC TGAATAACCA GTGGAACAGA 
CTGCAGAACA TCAAGAACAC AGTAGACAAC ACAGGAGCAC AGGCAGACAG AGCACGAAAC 
CGCGTTCGTG ATGCAGAAAA CCTGATCAAC ACAGCCAGAG AGGAGCTGGA CAAAGCCAAA 
GAAGCCATCA GTAAAGTGGA CATTAAAATA CCTACAACCT CTGGAGATCC AAACAATATG 
ACGCTTCTGG CAGAGGAAGC CCGTAAGCTG TCAGAAAAAC ATAAAGCAGA TGCAGATCAG 
ATTGAGAAGA TCGCGAAAGA TGCCAATGAC ACTTCTACTA AAGCCTATAA TATGCTGAAG 
AAAGCACTTG ATGGAGAGAA CAAGACAAGC AGCGACATTG ATGAACTCAA CAGGAAGTAT 
TTGGAAGCGA AAGATCTGGC CAAAAATCTG GAGAAGCAGG CAGCTAAAGT TCATGCTGAG 
GCAGAAGAGG CCGGAAACAA AGCTCTGAAG ATCTATGCTA ACCTGACCAG CTTACCTCCA 
ATCAACACCA AAACATTAGA GGATGATGCC AATAAGATTA AAAAGGAGGC TTCAGACTTG 
GACAAACTGA TAGACAAGAC TGAGAAGGAG TACAATGACT TGAGAGAAGA CTTGAGAGGA 
AAAGAGACGG AGGTCCGCAA ACTGCTGGAC AAAGGAAAAA CTGAGCAACA GACGGCTGAT 
CAGTTGTTGG CGCGTGCAGA CGCAGCAAAG GCTTTGGCTG AGGAGGCGGC AAAAAAAGGC 
AAATCTACTT TCCAAGAGGC TCAGGATATT CTGAACAACC TCAGAGATTT CGACAAGCGA 
GTGAATGACA ACAAAACTGC TGCTGAGGAT GCCATGAGGA GAATCCCACA AATCAATGCA 
ACAATCAATG AAGCCAATGA TAAAACCAGA CGGGCCGAGG CGGCTTTAGG CAATGCAGCT 
GCTGATGCTA AAGATGCTAA AGCGAAGGCA GAGGAAGCTG AGAAAATCGC TAATGATGTG 
CAGAAGGGTT CTGCAAAGAC CAAGGCAGAC GCAGAAAAGG CTTTTGAAGA CACCATGAAA 
CTGGATAAAG ATGTTGACAA AATGATGGAC CAGCTCACAG CAGCGGAGAA AGAGCTGGAA 
AAGAAGAAAG CAGAGGCGGA CACTGACATG ATGATGGCAT CTATGGCATC AGACAATGCC 
AAAGATGCAG AGGGCAATGC CAGAAAAGCC AAAAGTGCTG TCAGAGAAGT ACTCAACACA 
ATCAATGCAC TGCTAGGCCA ACTCGGTAAC ATCGATAAGG TGGATCTGAG CAAGCTGAAT 
CAGATCGACA ACGCTCTCAA AGATGCCAAG GACAAAATGG CGGGAAGTGA GCTGGACAGG 
AAGCTGAAGG AGCTGAACGA CATCGCCAAG AGTCAGGAGG ACATGATCAG CGACTACGAC 
CGTCAGATCC AGGAGATTCG AGCAGACATT GCCAACCTGA ACGACATCAA GAACACTCTG 
CCCGAGGGCT GCTTCAACAC GCCGTCTCTA GAGCGGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775384.1
CDS	371..5152
Translation	MSLFSCLLLWTLWAACSHGAMDECIDEDDRPQRCMPEFVNAAFNATVVATNTCGSPPEEFCVQTGVTGVTKSCHICNAADPRLHHGAVYLTDYNQPVQPTWWQSQTMLAGIQYPNSINLTLHLGKSFDITYVRLKFHTSRPESFAIYKRSSEDGPWTPYQYYSGSCEKTYSKNNRGFIRTGEDEQQALCTDEFSDISPLYGGNVAFSTLEGRPSAYNFDNSPVLQDWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCNGHASECVKNEYSKLVCNCKHNTEGADCNVCKPFYNDRPWRRATAENPNECLPCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPKCERCLANYYREASGQRCLSCGCNPVGSLSTQCDNTGRCSCKPGVMGDKCDRCQPGYHSLSEAGCRPCSCNPAGSTQECDVQTGRCQCKENVDGFNCDRCKLGYFNLDPQNPQGCTPCFCFQHSTVCESADGYSVHKITSTFDRDDEGWKGKQRDDSSVPVQWSPSSGEISLISEDYFPIYFVAPDKFLHNQLLSYGQNLTLNFRIQRHDARLSAEDVVLEGSGLRVAVPLIAQGNSYPGEETQTFVFRLHDTTDYPWRPTIKHADFQKLLYNLTSIMIRGTYSAQSAGYLDNVSLVTARRGPGTPARWVEKCTCPQGYLGQHCEQCDQGFRRSRPELRRFSTCERCNCNGHSDTCDPETGMCNCQHNTAGLSCERCKDGFYGDSTVGSSSDCKACPCPAGATCAVVPKTNEVVCTNCPTGTTGKRCELCDDGFFGDPLGEKGPVRACRACSCNNNIEPNAVGNCNRESGECLKCIYNTAGVFCDRCKQGFYGDARAANVADKCKPCKCSPYGTVDRQTACSQVTGQCPCLPHVINRDCGACELGFYNLQSGKGCERCNCNPIGSTNGQCDIVSGQCECQPGVTGQHCERCEVNFFGFSSSGCKPCDCDPEGSESAQCKEDGRCHCRPGFVGSRCDMCEENYFYNRSTPGCQQCPNCYSLVRDKVNQQRQKLLDLQNLIDSLDNTETTVSDKAFEDRLKEAEKTIMDLLEEAQASKEVDKGLLDRLNNINKTLNNQWNRLQNIKNTVDNTGAQADRARNRVRDAENLINTAREELDKAKEAISKVDIKIPTTSGDPNNMTLLAEEARKLSEKHKADADQIEKIAKDANDTSTKAYNMLKKALDGENKTSSDIDELNRKYLEAKDLAKNLEKQAAKVHAEAEEAGNKALKIYANLTSLPPINTKTLEDDANKIKKEASDLDKLIDKTEKEYNDLREDLRGKETEVRKLLDKGKTEQQTADQLLARADAAKALAEEAAKKGKSTFQEAQDILNNLRDFDKRVNDNKTAAEDAMRRIPQINATINEANDKTRRAEAALGNAAADAKDAKAKAEEAEKIANDVQKGSAKTKADAEKAFEDTMKLDKDVDKMMDQLTAAEKELEKKKAEADTDMMMASMASDNAKDAEGNARKAKSAVREVLNTINALLGQLGNIDKVDLSKLNQIDNALKDAKDKMAGSELDRKLKELNDIAKSQEDMISDYDRQIQEIRADIANLNDIKNTLPEGCFNTPSLERP

Target ORF information:

RefSeq Version	NM_173277.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, gamma 1 (lamc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173277.1

ORF Insert Sequence:

ATGTCGCTTT TTAGCTGTTT GTTGTTATGG ACTTTGTGGG CAGCGTGCAG TCACGGAGCG 
ATGGATGAAT GTATAGACGA GGATGATCGG CCTCAGCGCT GCATGCCGGA GTTCGTTAAC 
GCGGCTTTCA ATGCGACCGT GGTGGCCACC AACACTTGCG GCTCGCCGCC TGAAGAGTTC 
TGCGTACAGA CCGGGGTTAC GGGAGTCACT AAATCATGCC ACATTTGCAA TGCCGCGGAC 
CCCAGACTGC ACCACGGCGC TGTTTATCTC ACCGATTACA ACCAACCAGT ACAACCAACC 
TGGTGGCAAA GCCAGACTAT GTTAGCGGGC ATCCAGTACC CGAACTCCAT CAACTTGACC 
CTTCACCTGG GCAAATCTTT CGACATCACC TATGTGCGTT TGAAGTTCCA CACCAGCCGT 
CCTGAGAGTT TTGCCATCTA CAAGCGCTCC AGTGAAGACG GGCCTTGGAC CCCCTACCAA 
TACTACAGTG GCTCCTGTGA GAAAACCTAC AGCAAAAACA ACCGTGGCTT CATCCGCACG 
GGCGAGGACG AGCAGCAGGC TCTGTGTACA GATGAGTTTA GCGACATCTC GCCACTCTAT 
GGAGGAAACG TGGCTTTCTC TACGCTCGAG GGCCGACCCA GTGCATATAA CTTCGACAAC 
AGCCCTGTAC TGCAGGATTG GGTAACTGCC ACTGATATCA GAGTGACCCT GAATCGATTA 
AACACTTTCG GAGATGAAGT TTTCAACGAT CCCAAAGTCC TCAAATCCTA CTACTACGCC 
ATCTCGGATT TTGCTGTGGG AGGAAGGTGC AAGTGCAATG GCCATGCCAG CGAGTGCGTG 
AAGAACGAGT ACAGCAAGCT GGTGTGTAAC TGCAAACACA ACACAGAAGG AGCCGACTGC 
AATGTGTGCA AACCCTTCTA TAATGACCGT CCCTGGAGGA GAGCCACTGC TGAAAACCCT 
AATGAGTGTC TGCCTTGTAA CTGTAATGGA AAGAGCGCTG AATGTTACTT CGACCCTGAG 
CTGTATCGTG CCACTGGTCA CGGAGGTCAC TGCCGTAACT GTGCTGACAA TACAGATGGG 
CCGAAGTGTG AGCGATGCCT GGCCAACTAC TACAGAGAGG CCTCCGGTCA GCGCTGTCTG 
TCCTGCGGCT GCAACCCTGT TGGGTCTCTC AGCACTCAGT GTGATAACAC TGGCAGATGC 
AGCTGTAAGC CAGGAGTGAT GGGAGACAAG TGTGACAGAT GCCAACCTGG ATACCACTCG 
CTCTCTGAGG CAGGATGCAG ACCGTGCTCT TGTAATCCAG CCGGAAGCAC ACAGGAGTGT 
GATGTCCAGA CCGGACGCTG CCAGTGCAAG GAAAATGTGG ACGGATTCAA CTGTGACCGG 
TGTAAACTGG GCTATTTTAA TCTGGACCCC CAGAATCCTC AGGGCTGCAC TCCATGCTTC 
TGCTTCCAGC ATTCGACAGT GTGTGAGAGC GCAGACGGCT ACAGTGTGCA CAAAATCACC 
TCCACCTTTG ACAGAGATGA TGAAGGCTGG AAGGGTAAAC AGAGAGATGA CTCCAGTGTA 
CCGGTACAGT GGTCACCCAG TTCTGGAGAG ATCTCACTCA TCTCAGAAGA TTACTTCCCC 
ATCTACTTTG TGGCTCCAGA TAAATTTCTG CATAATCAGC TGTTGAGTTA TGGTCAGAAC 
CTCACGCTGA ACTTCAGGAT TCAGCGTCAC GATGCTCGCC TCTCAGCAGA GGACGTTGTG 
CTGGAGGGCT CGGGGCTTCG TGTCGCTGTG CCTCTTATTG CTCAGGGTAA TTCCTACCCA 
GGAGAGGAGA CCCAAACCTT TGTGTTCAGA CTACATGACA CAACTGACTA CCCTTGGAGA 
CCCACCATAA AGCATGCAGA CTTCCAGAAA CTTCTCTACA ATCTGACTTC AATCATGATC 
CGTGGAACCT ACAGCGCACA AAGTGCTGGT TATTTGGATA ACGTGTCTCT GGTGACTGCC 
CGCCGGGGCC CTGGCACTCC GGCCCGATGG GTTGAGAAAT GCACGTGTCC GCAGGGTTAT 
TTGGGTCAGC ATTGTGAGCA GTGCGATCAG GGATTCAGGA GGAGCCGGCC AGAGCTGCGA 
CGCTTCAGCA CCTGCGAGCG CTGCAACTGC AATGGACACA GCGACACATG TGACCCTGAG 
ACAGGAATGT GTAACTGCCA GCACAACACT GCTGGTTTGA GCTGTGAAAG GTGTAAAGAC 
GGCTTCTATG GTGATTCTAC AGTGGGCTCT TCTTCAGACT GTAAAGCCTG TCCCTGCCCT 
GCTGGTGCCA CCTGTGCTGT GGTGCCCAAG ACCAATGAGG TGGTCTGTAC CAACTGCCCG 
ACTGGAACCA CAGGTAAACG ATGTGAGCTG TGTGACGATG GTTTCTTTGG AGATCCTCTG 
GGTGAGAAAG GTCCAGTGAG AGCGTGTCGA GCCTGCAGCT GCAACAACAA CATCGAGCCG 
AACGCTGTGG GCAACTGTAA CCGAGAGTCG GGCGAATGCC TCAAGTGCAT CTACAACACC 
GCTGGCGTCT TTTGCGACCG CTGCAAGCAG GGCTTCTATG GTGATGCCCG CGCTGCTAAT 
GTAGCCGACA AATGCAAACC GTGCAAGTGT TCTCCATATG GTACAGTAGA CCGACAGACA 
GCCTGTTCTC AGGTGACGGG TCAATGTCCG TGTCTCCCTC ACGTCATTAA CAGAGACTGT 
GGCGCTTGTG AACTGGGTTT CTACAATCTG CAGAGTGGAA AGGGCTGTGA ACGATGCAAC 
TGCAACCCTA TTGGTTCCAC GAATGGTCAG TGTGACATTG TCAGCGGTCA GTGTGAGTGT 
CAGCCTGGAG TCACCGGTCA ACATTGTGAG CGATGCGAGG TCAACTTCTT CGGATTCAGT 
TCATCGGGTT GCAAACCATG TGACTGTGAC CCAGAAGGCT CAGAATCAGC TCAGTGTAAG 
GAGGACGGCC GCTGTCATTG TCGGCCAGGT TTTGTGGGCT CTCGCTGTGA TATGTGTGAG 
GAAAACTACT TCTACAACCG CTCGACACCT GGCTGCCAGC AGTGCCCTAA CTGTTACAGC 
CTGGTCAGAG ACAAGGTAAA CCAGCAGAGA CAAAAACTGC TTGACCTGCA GAATTTGATT 
GATAGTCTGG ACAACACTGA GACGACAGTG AGCGACAAGG CCTTTGAGGA CAGACTGAAG 
GAGGCAGAGA AAACTATAAT GGACTTGCTG GAAGAGGCTC AGGCTAGCAA AGAAGTGGAT 
AAAGGTCTTT TGGACCGTCT AAACAACATC AACAAGACCC TGAATAACCA GTGGAACAGA 
CTGCAGAACA TCAAGAACAC AGTAGACAAC ACAGGAGCAC AGGCAGACAG AGCACGAAAC 
CGCGTTCGTG ATGCAGAAAA CCTGATCAAC ACAGCCAGAG AGGAGCTGGA CAAAGCCAAA 
GAAGCCATCA GTAAAGTGGA CATTAAAATA CCTACAACCT CTGGAGATCC AAACAATATG 
ACGCTTCTGG CAGAGGAAGC CCGTAAGCTG TCAGAAAAAC ATAAAGCAGA TGCAGATCAG 
ATTGAGAAGA TCGCGAAAGA TGCCAATGAC ACTTCTACTA AAGCCTATAA TATGCTGAAG 
AAAGCACTTG ATGGAGAGAA CAAGACAAGC AGCGACATTG ATGAACTCAA CAGGAAGTAT 
TTGGAAGCGA AAGATCTGGC CAAAAATCTG GAGAAGCAGG CAGCTAAAGT TCATGCTGAG 
GCAGAAGAGG CCGGAAACAA AGCTCTGAAG ATCTATGCTA ACCTGACCAG CTTACCTCCA 
ATCAACACCA AAACATTAGA GGATGATGCC AATAAGATTA AAAAGGAGGC TTCAGACTTG 
GACAAACTGA TAGACAAGAC TGAGAAGGAG TACAATGACT TGAGAGAAGA CTTGAGAGGA 
AAAGAGACGG AGGTCCGCAA ACTGCTGGAC AAAGGAAAAA CTGAGCAACA GACGGCTGAT 
CAGTTGTTGG CGCGTGCAGA CGCAGCAAAG GCTTTGGCTG AGGAGGCGGC AAAAAAAGGC 
AAATCTACTT TCCAAGAGGC TCAGGATATT CTGAACAACC TCAGAGATTT CGACAAGCGA 
GTGAATGACA ACAAAACTGC TGCTGAGGAT GCCATGAGGA GAATCCCACA AATCAATGCA 
ACAATCAATG AAGCCAATGA TAAAACCAGA CGGGCCGAGG CGGCTTTAGG CAATGCAGCT 
GCTGATGCTA AAGATGCTAA AGCGAAGGCA GAGGAAGCTG AGAAAATCGC TAATGATGTG 
CAGAAGGGTT CTGCAAAGAC CAAGGCAGAC GCAGAAAAGG CTTTTGAAGA CACCATGAAA 
CTGGATAAAG ATGTTGACAA AATGATGGAC CAGCTCACAG CAGCGGAGAA AGAGCTGGAA 
AAGAAGAAAG CAGAGGCGGA CACTGACATG ATGATGGCAT CTATGGCATC AGACAATGCC 
AAAGATGCAG AGGGCAATGC CAGAAAAGCC AAAAGTGCTG TCAGAGAAGT ACTCAACACA 
ATCAATGCAC TGCTAGGCCA ACTCGGTAAC ATCGATAAGG TGGATCTGAG CAAGCTGAAT 
CAGATCGACA ACGCTCTCAA AGATGCCAAG GACAAAATGG CGGGAAGTGA GCTGGACAGG 
AAGCTGAAGG AGCTGAACGA CATCGCCAAG AGTCAGGAGG ACATGATCAG CGACTACGAC 
CGTCAGATCC AGGAGATTCG AGCAGACATT GCCAACCTGA ACGACATCAA GAACACTCTG 
CCCGAGGGCT GCTTCAACAC GCCGTCTCTA GAGCGGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Science advances2(11)e1600844(2016 Nov)
Chen WC,Wang Z,Missinato MA,Park DW,Long DW,Liu HJ,Zeng X,Yates NA,Kim K,Wang Y

Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
Skeletal muscle618(2016)
Jenkins MH,Alrowaished SS,Goody MF,Crawford BD,Henry CA

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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lamc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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