dag1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dag1
Entrez Gene ID	286829
Full Name	dystroglycan 1
Synonyms	dag,wu:fb83d06,wu:fi25f06,wu:fi37b08,zgc:109786
General protein information	Preferred Names dystroglycan 1 Names dystroglycan dystrophin-associated glycoprote dystrophin-associated glycoprotein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170544.4	XP_005170601.1	dystroglycan isoform X1
NM_173274.1	NP_775381.1	dystroglycan precursor
XM_009296487.2	XP_009294762.1	dystroglycan isoform X1
XM_005170544.3	XP_005170601.1	dystroglycan isoform X1
XM_009296488.2	XP_009294763.1	dystroglycan isoform X1
XM_009296487.3	XP_009294762.1	dystroglycan isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DAG1	NP_776587.1
Danio rerio (zebrafish)	dag1	NP_775381.1
Canis lupus familiaris (dog)	DAG1	NP_001029164.1
Homo sapiens (human)	DAG1	NP_001171105.1
Mus musculus (house mouse)	Dag1	NP_001263421.1
Rattus norvegicus (Norway rat)	Dag1	NP_446149.1
Pan troglodytes (chimpanzee)	DAG1	XP_003339091.1
	DAG1	XP_001108436.1
Gallus gallus (chicken)	DAG1	NP_001091009.1
Xenopus tropicalis (tropical clawed frog)	dag1	NP_001016518.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Characterization of Zebrafish Models of Marinesco-Sj?gren Syndrome.
Kawahara G, Hayashi YK
PloS one11(10)e0165563(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patchytail fish contain a point mutation (c.1700T>A) in dag1, resulting in a missense change leading to skeletal muscle defects, brain abnormalities and ocular defects in posterior as well as anterior chambers.
Title: The zebrafish dag1 mutant: a novel genetic model for dystroglycanopathies.

genetic evidence that neuromuscular synapse formation can occur in the absence of MuSK and that the combinatorial function of UnpFL/MuSK and dystroglycan generates diverse patterns of vertebrate neuromuscular innervation
Title: Differential requirement for MuSK and dystroglycan in generating patterns of neuromuscular innervation.

Removal of dystroglycan causes severe muscular dystrophy in zebrafish embryos.
Title: Removal of dystroglycan causes severe muscular dystrophy in zebrafish embryos.

The following dag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35760	NM_173274.1 Latest version!	Danio rerio dystroglycan 1 (dag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27176	XM_009296487.3 Latest version!	Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27176	XM_005170544.4 Latest version!	Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27176	XM_009296488.2 Latest version!	Danio rerio dystroglycan 1 (dag1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa27176	XM_005170544.3	Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27176	XM_009296487.2	Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dag1 ( NM_173274.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775381.1 Danio rerio dystroglycan 1 (dag1), mRNA.

Price & Availability↑

CloneID	ODa35760
Clone ID Related Accession (Same CDS sequence)	NM_173274.1
Accession Version	NM_173274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Danio rerio(zebrafish)
Product	dystroglycan precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF483476.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF483476.1, BC099987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCAGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTTCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG GGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCGT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775381.1
CDS	377..2977
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVQASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	NM_173274.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173274.1

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCAGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTTCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG GGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCGT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dag1 ( XM_005170544.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170601.1 Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27176
Clone ID Related Accession (Same CDS sequence)	XM_005170544.4 , XM_009296487.2 , XM_005170544.3 , XM_009296488.2 , XM_009296487.3
Accession Version	XM_005170544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170544.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170601.1
CDS	667..3267
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVRASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005170544.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170544.4

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dag1 ( XM_009296487.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294762.1 Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27176
Clone ID Related Accession (Same CDS sequence)	XM_005170544.4 , XM_009296487.2 , XM_005170544.3 , XM_009296488.2 , XM_009296487.3
Accession Version	XM_009296487.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dystroglycan isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294762.1
CDS	293..2893
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVRASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_009296487.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296487.2

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dag1 ( XM_005170544.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170601.1 Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27176
Clone ID Related Accession (Same CDS sequence)	XM_005170544.4 , XM_009296487.2 , XM_005170544.3 , XM_009296488.2 , XM_009296487.3
Accession Version	XM_005170544.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dystroglycan isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170601.1
CDS	674..3274
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVRASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005170544.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170544.3

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dag1 ( XM_009296488.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294763.1 Danio rerio dystroglycan 1 (dag1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27176
Clone ID Related Accession (Same CDS sequence)	XM_005170544.4 , XM_009296487.2 , XM_005170544.3 , XM_009296488.2 , XM_009296487.3
Accession Version	XM_009296488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dystroglycan isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294763.1
CDS	328..2928
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVRASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_009296488.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296488.2

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dag1 ( XM_009296487.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294762.1 Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27176
Clone ID Related Accession (Same CDS sequence)	XM_005170544.4 , XM_009296487.2 , XM_005170544.3 , XM_009296488.2 , XM_009296487.3
Accession Version	XM_009296487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294762.1
CDS	776..3376
Translation	MRNKLREFRDGGRPMLGQWDRTLVVLLMALALTVTDVRASWDQMEVLGDVKLEASMHSAVLSDIREAEAVVAAVSQPSGFPDSSAVVGRVFRLQVPIKAKDSGSIVKITEASKDVLPAWLHWDAESGTLQGLPLETDKGVHYISVSVSNESKVSQSPDVFSIEVHPEEHAETDPFQLAIQQSASNDAQPFICGNEEPVTVLTVILDADLTKMSSKQRVELLAKMKKFSGMGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSSIPDISSVQVPAKEGTMSAQLGYPVVGWHIANKKPHVPKRVRRQLNNTPTPIPSLLPPTTYPEPPIRIVPTPTSPSIAPTSESSAPPVRGPVPLPVKPTIRNRDSAPSTPTLGPPLPTRVMVTTSTIAIQPTMTRPVYVGASVTPATPTTRKPTKRPPKKPKTTPIPREPKTTTTKPPRRSTPAVIGDSNIKPELRNPIDQVNAYVGTYFEVKVPSDTFFDKEDGTTDKLRLTLRKGNDVVADDSWIQFNSTSQLLYGLPDQEHAGKHEYFMQATDKGGLYAMDAFEVRVSMWGNSVKPPVLFTAVFDGDARTVTNDIHKKILLVKKLSQSFGDRNSSTITLKSITKGSIIVEWTNNSLQQSPCPKDQIQQLSKKISDPEGKPSSIFKFTMEPDFRPSNITVRGTASCRNYMFVPLGEIPDPTPSPGTPAVGAGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQDLLGEYTPLHDEDPNAPPYQPPPPFSTPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_009296487.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dystroglycan 1 (dag1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296487.3

ORF Insert Sequence:

ATGCGCAATA AACTCAGAGA GTTCCGGGAT GGGGGACGCC CCATGCTGGG ACAGTGGGAC 
AGGACTCTTG TGGTGCTGCT GATGGCTTTG GCGCTGACAG TAACGGACGT CCGGGCTTCC 
TGGGATCAGA TGGAGGTTCT AGGAGATGTA AAGTTGGAGG CGTCTATGCA CTCTGCCGTG 
CTTTCGGACA TCCGGGAGGC GGAAGCAGTG GTTGCGGCAG TGTCACAGCC TAGCGGATTC 
CCAGACTCCT CTGCGGTGGT GGGCAGGGTG TTTCGATTGC AGGTGCCCAT CAAAGCGAAG 
GACTCCGGGA GCATAGTAAA GATCACAGAA GCAAGCAAGG ATGTGCTCCC AGCGTGGTTG 
CATTGGGATG CAGAGAGCGG CACACTTCAA GGCCTTCCAC TAGAGACGGA CAAGGGCGTT 
CACTACATAT CTGTTTCAGT TTCCAACGAG AGTAAAGTCT CCCAAAGCCC AGATGTGTTC 
TCCATTGAGG TGCACCCAGA GGAGCACGCT GAGACGGACC CTTTTCAGCT TGCAATCCAG 
CAATCAGCAA GCAACGATGC CCAGCCTTTC ATCTGTGGCA ATGAGGAGCC TGTTACTGTG 
CTCACTGTCA TCCTTGACGC TGACCTCACA AAGATGAGCT CGAAGCAGAG GGTGGAACTA 
CTTGCCAAAA TGAAAAAGTT TTCTGGCATG GGGCTCCAGC ACATGAAGAT TTTGCCTGTG 
GTCAACAACC GCTTGTTTGA CATGTCTGCT TTCATGGCAG GACCAGGAAA TGCCAAGAAA 
GTGGTTGAAA ACGGAGCTCT TTTGTCCTGG AAGCTCGGCT GCAGTCTGGA TCAAAGCAGC 
ATTCCTGACA TTAGCAGTGT TCAGGTTCCA GCAAAAGAGG GGACAATGTC AGCGCAGTTG 
GGATATCCGG TTGTTGGATG GCATATTGCT AATAAAAAGC CCCATGTGCC CAAAAGGGTG 
CGAAGGCAGC TCAACAACAC CCCCACTCCC ATTCCCTCAT TACTCCCTCC TACAACTTAC 
CCTGAGCCCC CGATCCGCAT CGTTCCAACT CCAACCTCTC CATCTATTGC TCCTACCTCA 
GAGAGTTCTG CCCCACCTGT CCGTGGTCCC GTGCCCCTTC CTGTGAAACC CACCATTCGA 
AACAGGGATT CCGCGCCCTC CACTCCAACC TTAGGCCCCC CTCTGCCAAC ACGAGTAATG 
GTCACCACCA GCACTATCGC CATCCAGCCA ACAATGACCA GGCCTGTGTA TGTTGGAGCC 
TCTGTTACCC CAGCAACACC TACCACCAGG AAACCTACAA AGAGACCTCC AAAGAAACCC 
AAGACAACGC CCATACCAAG AGAACCCAAG ACCACGACAA CCAAGCCACC AAGGCGCAGC 
ACTCCTGCAG TTATAGGCGA TAGCAATATA AAACCTGAGC TGCGTAACCC AATTGATCAG 
GTGAATGCCT ATGTTGGCAC CTATTTTGAG GTGAAGGTTC CATCGGATAC ATTTTTTGAC 
AAAGAGGATG GAACTACCGA TAAGTTGAGG CTCACTTTGC GCAAGGGCAA TGATGTGGTC 
GCGGATGATT CCTGGATACA GTTTAATAGC ACAAGCCAGT TACTGTATGG ATTACCTGAC 
CAAGAGCATG CAGGGAAACA TGAGTATTTC ATGCAAGCAA CTGATAAAGG AGGTCTCTAT 
GCAATGGATG CCTTTGAGGT CCGTGTCAGC ATGTGGGGAA ACAGTGTTAA GCCACCTGTA 
TTATTTACTG CTGTGTTTGA TGGAGATGCG CGTACGGTCA CCAATGACAT TCACAAGAAG 
ATCCTTCTCG TCAAGAAACT GTCCCAGTCT TTTGGTGATC GCAACAGCAG CACCATTACA 
CTCAAGAGCA TCACCAAGGG TTCCATCATA GTGGAATGGA CCAACAACAG CCTTCAGCAA 
AGCCCCTGCC CAAAAGACCA AATACAGCAA TTGAGCAAAA AAATCTCCGA TCCAGAGGGC 
AAACCCTCGT CGATTTTCAA ATTTACCATG GAACCAGACT TCAGGCCCTC AAATATCACC 
GTCAGAGGCA CAGCAAGCTG TCGTAACTAC ATGTTTGTCC CGTTGGGTGA GATCCCAGAT 
CCGACCCCCT CTCCTGGTAC TCCCGCTGTG GGTGCGGGAA GACAGAGCAC TGATGATGTG 
TATCTTCACA CTGTCATACC AGCGGTGGTG GTTGCAGCCA TTTTGCTGAT AGCTGGAATC 
ATCGCCATGA TTTGCTACCG CAAGAAGCGC AAGGGCAAGC TAACAATCGA AGACCAAGCA 
ACTTTCATCA AGAAAGGAGT GCCCATCATT TTTGCAGATG AGCTCGATGA CTCTAAGCCT 
CCCCCATCTT CCAGCATGCC CCTTATCCTT CAGGAGGAGA AGCCTCCGCT TCCTCCGCCG 
GAGTATCCCA ACATGGCTAC TCCAGAGACA ACACCTCTGA ACCAGGACCT TTTGGGAGAG 
TACACACCTC TACATGATGA GGATCCCAAT GCCCCTCCCT ATCAGCCACC ACCACCCTTC 
TCCACCCCTA TGGAGGGGAA AGGATCCCGC CCCAAGAACA TGACCCCCTA CAGATCGCCA 
CCCCCTTACG TGCCACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Characterization of Zebrafish Models of Marinesco-Sj?gren Syndrome.
PloS one11(10)e0165563(2016)
Kawahara G,Hayashi YK

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dag1 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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