has3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	has3
Entrez Gene ID	282555
Full Name	hyaluronan synthase 3
Synonyms	dg42III,xx:af190743gs1,xx:af190743gs2
General protein information	Preferred Names hyaluronan synthase 3 Names hyaluronan synthase 3 af190743gs2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173220.2	NP_775327.2	hyaluronan synthase 3
XM_009293521.2	XP_009291796.1	hyaluronan synthase 3 isoform X1
XM_009293521.3	XP_009291796.1	hyaluronan synthase 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HAS3	NP_001186209.1
Bos taurus (cattle)	HAS3	NP_001179796.1
Mus musculus (house mouse)	Has3	NP_032243.2
Xenopus tropicalis (tropical clawed frog)	has3	XP_002936057.2
Pan troglodytes (chimpanzee)	HAS3	XP_511060.2
	HAS3	NP_001104247.1
Canis lupus familiaris (dog)	HAS3	XP_546859.2
Danio rerio (zebrafish)	has3	NP_775327.1
Gallus gallus (chicken)	HAS3	XP_425137.4
Rattus norvegicus (Norway rat)	Has3	NP_758822.1

Gene Ontology
Bibliography

Related articles in PubMed

Hyaluronic acid synthesis is required for zebrafish tail fin regeneration.
Ouyang X, Panetta NJ, Talbott MD, Payumo AY, Halluin C, Longaker MT, Chen JK
PloS one12(2)e0171898(2017)

Ancient origin of lubricated joints in bony vertebrates.
Askary A, Smeeton J, Paul S, Schindler S, Braasch I, Ellis NA, Postlethwait J, Miller CT, Crump JG
eLife5(2016 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration.
Govindan J, Tun KM, Iovine MK
PloS one11(2)e0148202(2016)

Extracellular component hyaluronic acid and its receptor Hmmr are required for epicardial EMT during heart regeneration.
Missinato MA, Tobita K, Romano N, Carroll JA, Tsang M
Cardiovascular research107(4)487-98(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following has3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the has3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23207	NM_173220.2 Latest version!	Danio rerio hyaluronan synthase 3 (has3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa23206	XM_009293521.3 Latest version!	Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa23206	XM_009293521.2	Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

has3 ( NM_173220.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775327.2 Danio rerio hyaluronan synthase 3 (has3), mRNA.

Price & Availability↑

CloneID	ODa23207
Clone ID Related Accession (Same CDS sequence)	NM_173220.2
Accession Version	NM_173220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Danio rerio(zebrafish)
Product	hyaluronan synthase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX950194.9. On Jun 25, 2015 this sequence version replaced NM_173220.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC163401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476750, SAMEA4476783 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCTC GCTTTGGCAC TGCGGTGCGG ATCTTCATCA CCACCTTATT TGCAGCAGTG 
GTGCTTTTCG CAATCCTACT AGCCTATGTG ACAGGTTACC AGTTCATCCA CACAGAGCAG 
CACCATCTGT CTTTTGGCTT GTACGGTGCA TTTCTATCCC TCCACCTTCT CCTGCAGAGT 
CTCTTCGCCT ACCTGGAGCA CAGACAAATG CGAGGCCCCT CCAGACCGCA GCACCTGCGC 
CGCACTGTGG CCCTCTGCAT TGCAGCCTAT CAGGAAGATC CAGACTACCT TCGCAAGTGT 
CTGCGGAGCA TTCGCCGCAT TTCTTTTCCC GGGCTGAAAG TGGTGTTGGT GGTGGATGGC 
AATCGGCAGG AGGATGCCTA CATGATGGAT ATCTTCCAGG AGGTGATGGG GGGAGTGGAG 
CAGACAGGCT GTGTGGTGTG GAAAGGGAAT TACCACAGTA ACGGGGATGG AGGAGGAGGA 
GGAGGGAAAG GCTCGGTGCA TGCCGAAGAG GCTGCACGAG TGGCCAGAGT GGTGCGGAGC 
TGCCGTTACT CCTGCATCAT GCAGGAATGG GGTGGCAAGA GGGAAGTGAT GTATACAGCC 
TTCAAAGCAC TTGGAGATAC AGTGGATTAC ATGCAGGTAT GTGATTCAGA CACTGTACTT 
GATCCAGCAT GCACTATTGA GATGTTGAAG ATTCTAGAGG AGGATCCTGA TGTGGGAGGA 
GTTGGAGGAG ATGTTCAGAT TCTGAACAAG TATGACTCCT GGATCTCCTT CCTGAGCAGT 
GTCCGCTATT GGATGGCCTT CAATGTGGAG CGGGCCTGCC AGTCTTATTT CGGATGTGTG 
CAGTGCATCA GTGGACCTCT AGGAATGTAC CGTAACTCTC TCCTTCAGCA GTTCTTAGAG 
CCCTGGTACC ACCAGACCTT CCTAGGAAGC AAGTGCAGTT TTGGTGATGA TCGACATCTC 
ACCAACCGCG TCCTTAGCTT TGGCTTCAAA ACCAAGTTCA CGGCACGCTC TCAATGCCAG 
ACCGAGACCC CAACACAATA CCTGCGTTGG CTCAATCAGC AGACCCGCTG GAGCAAGTCT 
TACTTTCGAG AGTGGCTCTA CAATGCTCTT TGGTTCCACA AGCACAGCCT GTGGATGACC 
TATGAATCTG TGGTTACTGG GTTCTTCCCC TTCTTTCTGG TGGCTACAGT CATCCACCTG 
TTCTACAGAG GCCGGTTGTG GAACATCTTA CTCTTCTTGT TGACGGTCCA GCTGGTGGGT 
ATGGTGAAGG CCACCTACGC CTGCTTCCTG CGGGGCAGCC TTGTCATGAT CTTCATGTCA 
CTCTACTCAC TGCTCTACAT GTCAAGCCTG CTCCCTGCTA AGATCTTTGC CCTGCTGACT 
ATTAACAAAG CAGGATGGGG CACATCAGGC AGGAAAAAGA TGGTAGTAAA CCTTATTGGA 
GCTGTGCCTG TTACTGTGTG GACAGCCATT CTGCTTGGTG GAGTGGTTTA CACCATTTAC 
TGCGAGGTTC AAGAACCTTT TACCGAGACT GAGAAGGCTC TTCTTATCGC AGGCACCATT 
GTCTATGCCT CTTACTGGCT TATACTCCTG GTGCTCTACC TGGCCATAGT GGCCAAACGT 
TGTAACAAAA GAGAAGAACA GTTCCACCTT TCCTATGCCG AAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775327.2
CDS	54..1721
Translation	MPSRFGTAVRIFITTLFAAVVLFAILLAYVTGYQFIHTEQHHLSFGLYGAFLSLHLLLQSLFAYLEHRQMRGPSRPQHLRRTVALCIAAYQEDPDYLRKCLRSIRRISFPGLKVVLVVDGNRQEDAYMMDIFQEVMGGVEQTGCVVWKGNYHSNGDGGGGGGKGSVHAEEAARVARVVRSCRYSCIMQEWGGKREVMYTAFKALGDTVDYMQVCDSDTVLDPACTIEMLKILEEDPDVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEPWYHQTFLGSKCSFGDDRHLTNRVLSFGFKTKFTARSQCQTETPTQYLRWLNQQTRWSKSYFREWLYNALWFHKHSLWMTYESVVTGFFPFFLVATVIHLFYRGRLWNILLFLLTVQLVGMVKATYACFLRGSLVMIFMSLYSLLYMSSLLPAKIFALLTINKAGWGTSGRKKMVVNLIGAVPVTVWTAILLGGVVYTIYCEVQEPFTETEKALLIAGTIVYASYWLILLVLYLAIVAKRCNKREEQFHLSYAEA

Target ORF information:

RefSeq Version	NM_173220.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hyaluronan synthase 3 (has3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173220.2

ORF Insert Sequence:

ATGCCCTCTC GCTTTGGCAC TGCGGTGCGG ATCTTCATCA CCACCTTATT TGCAGCAGTG 
GTGCTTTTCG CAATCCTACT AGCCTATGTG ACAGGTTACC AGTTCATCCA CACAGAGCAG 
CACCATCTGT CTTTTGGCTT GTACGGTGCA TTTCTATCCC TCCACCTTCT CCTGCAGAGT 
CTCTTCGCCT ACCTGGAGCA CAGACAAATG CGAGGCCCCT CCAGACCGCA GCACCTGCGC 
CGCACTGTGG CCCTCTGCAT TGCAGCCTAT CAGGAAGATC CAGACTACCT TCGCAAGTGT 
CTGCGGAGCA TTCGCCGCAT TTCTTTTCCC GGGCTGAAAG TGGTGTTGGT GGTGGATGGC 
AATCGGCAGG AGGATGCCTA CATGATGGAT ATCTTCCAGG AGGTGATGGG GGGAGTGGAG 
CAGACAGGCT GTGTGGTGTG GAAAGGGAAT TACCACAGTA ACGGGGATGG AGGAGGAGGA 
GGAGGGAAAG GCTCGGTGCA TGCCGAAGAG GCTGCACGAG TGGCCAGAGT GGTGCGGAGC 
TGCCGTTACT CCTGCATCAT GCAGGAATGG GGTGGCAAGA GGGAAGTGAT GTATACAGCC 
TTCAAAGCAC TTGGAGATAC AGTGGATTAC ATGCAGGTAT GTGATTCAGA CACTGTACTT 
GATCCAGCAT GCACTATTGA GATGTTGAAG ATTCTAGAGG AGGATCCTGA TGTGGGAGGA 
GTTGGAGGAG ATGTTCAGAT TCTGAACAAG TATGACTCCT GGATCTCCTT CCTGAGCAGT 
GTCCGCTATT GGATGGCCTT CAATGTGGAG CGGGCCTGCC AGTCTTATTT CGGATGTGTG 
CAGTGCATCA GTGGACCTCT AGGAATGTAC CGTAACTCTC TCCTTCAGCA GTTCTTAGAG 
CCCTGGTACC ACCAGACCTT CCTAGGAAGC AAGTGCAGTT TTGGTGATGA TCGACATCTC 
ACCAACCGCG TCCTTAGCTT TGGCTTCAAA ACCAAGTTCA CGGCACGCTC TCAATGCCAG 
ACCGAGACCC CAACACAATA CCTGCGTTGG CTCAATCAGC AGACCCGCTG GAGCAAGTCT 
TACTTTCGAG AGTGGCTCTA CAATGCTCTT TGGTTCCACA AGCACAGCCT GTGGATGACC 
TATGAATCTG TGGTTACTGG GTTCTTCCCC TTCTTTCTGG TGGCTACAGT CATCCACCTG 
TTCTACAGAG GCCGGTTGTG GAACATCTTA CTCTTCTTGT TGACGGTCCA GCTGGTGGGT 
ATGGTGAAGG CCACCTACGC CTGCTTCCTG CGGGGCAGCC TTGTCATGAT CTTCATGTCA 
CTCTACTCAC TGCTCTACAT GTCAAGCCTG CTCCCTGCTA AGATCTTTGC CCTGCTGACT 
ATTAACAAAG CAGGATGGGG CACATCAGGC AGGAAAAAGA TGGTAGTAAA CCTTATTGGA 
GCTGTGCCTG TTACTGTGTG GACAGCCATT CTGCTTGGTG GAGTGGTTTA CACCATTTAC 
TGCGAGGTTC AAGAACCTTT TACCGAGACT GAGAAGGCTC TTCTTATCGC AGGCACCATT 
GTCTATGCCT CTTACTGGCT TATACTCCTG GTGCTCTACC TGGCCATAGT GGCCAAACGT 
TGTAACAAAA GAGAAGAACA GTTCCACCTT TCCTATGCCG AAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

has3 ( XM_009293521.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291796.1 Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23206
Clone ID Related Accession (Same CDS sequence)	XM_009293521.2 , XM_009293521.3
Accession Version	XM_009293521.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hyaluronan synthase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAA TGCCCTCTCG CTTTGGCACT GCGGTGCGGA TCTTCATCAC CACCTTATTT 
GCAGCAGTGG TGCTTTTCGC AATCCTACTA GCCTATGTGA CAGGTTACCA GTTCATCCAC 
ACAGAGCAGC ACCATCTGTC TTTTGGCTTG TACGGTGCAT TTCTATCCCT CCACCTTCTC 
CTGCAGAGTC TCTTCGCCTA CCTGGAGCAC AGACAAATGC GAGGCCCCTC CAGACCGCAG 
CACCTGCGCC GCACTGTGGC CCTCTGCATT GCAGCCTATC AGGAAGATCC AGACTACCTT 
CGCAAGTGTC TGCGGAGCAT TCGCCGCATT TCTTTTCCCG GGCTGAAAGT GGTGTTGGTG 
GTGGATGGCA ATCGGCAGGA GGATGCCTAC ATGATGGATA TCTTCCAGGA GGTGATGGGG 
GGAGTGGAGC AGACAGGCTG TGTGGTGTGG AAAGGGAATT ACCACAGTAA CGGGGATGGA 
GGAGGAGGAG GAGGGAAAGG CTCGGTGCAT GCCGAAGAGG CTGCACGAGT GGCCAGAGTG 
GTGCGGAGCT GCCGTTACTC CTGCATCATG CAGGAATGGG GTGGCAAGAG GGAAGTGATG 
TATACAGCCT TCAAAGCACT TGGAGATACA GTGGATTACA TGCAGGTATG TGATTCAGAC 
ACTGTACTTG ATCCAGCATG CACTATTGAG ATGTTGAAGA TTCTAGAGGA GGATCCTGAT 
GTGGGAGGAG TTGGAGGAGA TGTTCAGATT CTGAACAAGT ATGACTCCTG GATCTCCTTC 
CTGAGCAGTG TCCGCTATTG GATGGCCTTC AATGTGGAGC GGGCCTGCCA GTCTTATTTC 
GGATGTGTGC AGTGCATCAG TGGACCTCTA GGAATGTACC GTAACTCTCT CCTTCAGCAG 
TTCTTAGAGC CCTGGTACCA CCAGACCTTC CTAGGAAGCA AGTGCAGTTT TGGTGATGAT 
CGACATCTCA CCAACCGCGT CCTTAGCTTT GGCTTCAAAA CCAAGTTCAC GGCACGCTCT 
CAATGCCAGA CCGAGACCCC AACACAATAC CTGCGTTGGC TCAATCAGCA GACCCGCTGG 
AGCAAGTCTT ACTTTCGAGA GTGGCTCTAC AATGCTCTTT GGTTCCACAA GCACAGCCTG 
TGGATGACCT ATGAATCTGT GGTTACTGGG TTCTTCCCCT TCTTTCTGGT GGCTACAGTC 
ATCCACCTGT TCTACAGAGG CCGGTTGTGG AACATCTTAC TCTTCTTGTT GACGGTCCAG 
CTGGTGGGTA TGGTGAAGGC CACCTACGCC TGCTTCCTGC GGGGCAGCCT TGTCATGATC 
TTCATGTCAC TCTACTCACT GCTCTACATG TCAAGCCTGC TCCCTGCTAA GATCTTTGCC 
CTGCTGACTA TTAACAAAGC AGGATGGGGC ACATCAGGCA GGAAAAAGAT GGTAGTAAAC 
CTTATTGGAG CTGTGCCTGT TACTGTGTGG ACAGCCATTC TGCTTGGTGG AGTGGTTTAC 
ACCATTTACT GCGAGGTTCA AGAACCTTTT ACCGAGACTG AGAAGGCTCT TCTTATCGCA 
GGCACCATTG TCTATGCCTC TTACTGGCTT ATACTCCTGG TGCTCTACCT GGCCATAGTG 
GCCAAACGTT GTAACAAAAG AGAAGAACAG TTCCACCTTT CCTATGCCGA AGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291796.1
CDS	824..2500
Translation	MTTMPSRFGTAVRIFITTLFAAVVLFAILLAYVTGYQFIHTEQHHLSFGLYGAFLSLHLLLQSLFAYLEHRQMRGPSRPQHLRRTVALCIAAYQEDPDYLRKCLRSIRRISFPGLKVVLVVDGNRQEDAYMMDIFQEVMGGVEQTGCVVWKGNYHSNGDGGGGGGKGSVHAEEAARVARVVRSCRYSCIMQEWGGKREVMYTAFKALGDTVDYMQVCDSDTVLDPACTIEMLKILEEDPDVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEPWYHQTFLGSKCSFGDDRHLTNRVLSFGFKTKFTARSQCQTETPTQYLRWLNQQTRWSKSYFREWLYNALWFHKHSLWMTYESVVTGFFPFFLVATVIHLFYRGRLWNILLFLLTVQLVGMVKATYACFLRGSLVMIFMSLYSLLYMSSLLPAKIFALLTINKAGWGTSGRKKMVVNLIGAVPVTVWTAILLGGVVYTIYCEVQEPFTETEKALLIAGTIVYASYWLILLVLYLAIVAKRCNKREEQFHLSYAEA

Target ORF information:

RefSeq Version	XM_009293521.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293521.2

ORF Insert Sequence:

ATGACTACAA TGCCCTCTCG CTTTGGCACT GCGGTGCGGA TCTTCATCAC CACCTTATTT 
GCAGCAGTGG TGCTTTTCGC AATCCTACTA GCCTATGTGA CAGGTTACCA GTTCATCCAC 
ACAGAGCAGC ACCATCTGTC TTTTGGCTTG TACGGTGCAT TTCTATCCCT CCACCTTCTC 
CTGCAGAGTC TCTTCGCCTA CCTGGAGCAC AGACAAATGC GAGGCCCCTC CAGACCGCAG 
CACCTGCGCC GCACTGTGGC CCTCTGCATT GCAGCCTATC AGGAAGATCC AGACTACCTT 
CGCAAGTGTC TGCGGAGCAT TCGCCGCATT TCTTTTCCCG GGCTGAAAGT GGTGTTGGTG 
GTGGATGGCA ATCGGCAGGA GGATGCCTAC ATGATGGATA TCTTCCAGGA GGTGATGGGG 
GGAGTGGAGC AGACAGGCTG TGTGGTGTGG AAAGGGAATT ACCACAGTAA CGGGGATGGA 
GGAGGAGGAG GAGGGAAAGG CTCGGTGCAT GCCGAAGAGG CTGCACGAGT GGCCAGAGTG 
GTGCGGAGCT GCCGTTACTC CTGCATCATG CAGGAATGGG GTGGCAAGAG GGAAGTGATG 
TATACAGCCT TCAAAGCACT TGGAGATACA GTGGATTACA TGCAGGTATG TGATTCAGAC 
ACTGTACTTG ATCCAGCATG CACTATTGAG ATGTTGAAGA TTCTAGAGGA GGATCCTGAT 
GTGGGAGGAG TTGGAGGAGA TGTTCAGATT CTGAACAAGT ATGACTCCTG GATCTCCTTC 
CTGAGCAGTG TCCGCTATTG GATGGCCTTC AATGTGGAGC GGGCCTGCCA GTCTTATTTC 
GGATGTGTGC AGTGCATCAG TGGACCTCTA GGAATGTACC GTAACTCTCT CCTTCAGCAG 
TTCTTAGAGC CCTGGTACCA CCAGACCTTC CTAGGAAGCA AGTGCAGTTT TGGTGATGAT 
CGACATCTCA CCAACCGCGT CCTTAGCTTT GGCTTCAAAA CCAAGTTCAC GGCACGCTCT 
CAATGCCAGA CCGAGACCCC AACACAATAC CTGCGTTGGC TCAATCAGCA GACCCGCTGG 
AGCAAGTCTT ACTTTCGAGA GTGGCTCTAC AATGCTCTTT GGTTCCACAA GCACAGCCTG 
TGGATGACCT ATGAATCTGT GGTTACTGGG TTCTTCCCCT TCTTTCTGGT GGCTACAGTC 
ATCCACCTGT TCTACAGAGG CCGGTTGTGG AACATCTTAC TCTTCTTGTT GACGGTCCAG 
CTGGTGGGTA TGGTGAAGGC CACCTACGCC TGCTTCCTGC GGGGCAGCCT TGTCATGATC 
TTCATGTCAC TCTACTCACT GCTCTACATG TCAAGCCTGC TCCCTGCTAA GATCTTTGCC 
CTGCTGACTA TTAACAAAGC AGGATGGGGC ACATCAGGCA GGAAAAAGAT GGTAGTAAAC 
CTTATTGGAG CTGTGCCTGT TACTGTGTGG ACAGCCATTC TGCTTGGTGG AGTGGTTTAC 
ACCATTTACT GCGAGGTTCA AGAACCTTTT ACCGAGACTG AGAAGGCTCT TCTTATCGCA 
GGCACCATTG TCTATGCCTC TTACTGGCTT ATACTCCTGG TGCTCTACCT GGCCATAGTG 
GCCAAACGTT GTAACAAAAG AGAAGAACAG TTCCACCTTT CCTATGCCGA AGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

has3 ( XM_009293521.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291796.1 Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23206
Clone ID Related Accession (Same CDS sequence)	XM_009293521.2 , XM_009293521.3
Accession Version	XM_009293521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hyaluronan synthase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAA TGCCCTCTCG CTTTGGCACT GCGGTGCGGA TCTTCATCAC CACCTTATTT 
GCAGCAGTGG TGCTTTTCGC AATCCTACTA GCCTATGTGA CAGGTTACCA GTTCATCCAC 
ACAGAGCAGC ACCATCTGTC TTTTGGCTTG TACGGTGCAT TTCTATCCCT CCACCTTCTC 
CTGCAGAGTC TCTTCGCCTA CCTGGAGCAC AGACAAATGC GAGGCCCCTC CAGACCGCAG 
CACCTGCGCC GCACTGTGGC CCTCTGCATT GCAGCCTATC AGGAAGATCC AGACTACCTT 
CGCAAGTGTC TGCGGAGCAT TCGCCGCATT TCTTTTCCCG GGCTGAAAGT GGTGTTGGTG 
GTGGATGGCA ATCGGCAGGA GGATGCCTAC ATGATGGATA TCTTCCAGGA GGTGATGGGG 
GGAGTGGAGC AGACAGGCTG TGTGGTGTGG AAAGGGAATT ACCACAGTAA CGGGGATGGA 
GGAGGAGGAG GAGGGAAAGG CTCGGTGCAT GCCGAAGAGG CTGCACGAGT GGCCAGAGTG 
GTGCGGAGCT GCCGTTACTC CTGCATCATG CAGGAATGGG GTGGCAAGAG GGAAGTGATG 
TATACAGCCT TCAAAGCACT TGGAGATACA GTGGATTACA TGCAGGTATG TGATTCAGAC 
ACTGTACTTG ATCCAGCATG CACTATTGAG ATGTTGAAGA TTCTAGAGGA GGATCCTGAT 
GTGGGAGGAG TTGGAGGAGA TGTTCAGATT CTGAACAAGT ATGACTCCTG GATCTCCTTC 
CTGAGCAGTG TCCGCTATTG GATGGCCTTC AATGTGGAGC GGGCCTGCCA GTCTTATTTC 
GGATGTGTGC AGTGCATCAG TGGACCTCTA GGAATGTACC GTAACTCTCT CCTTCAGCAG 
TTCTTAGAGC CCTGGTACCA CCAGACCTTC CTAGGAAGCA AGTGCAGTTT TGGTGATGAT 
CGACATCTCA CCAACCGCGT CCTTAGCTTT GGCTTCAAAA CCAAGTTCAC GGCACGCTCT 
CAATGCCAGA CCGAGACCCC AACACAATAC CTGCGTTGGC TCAATCAGCA GACCCGCTGG 
AGCAAGTCTT ACTTTCGAGA GTGGCTCTAC AATGCTCTTT GGTTCCACAA GCACAGCCTG 
TGGATGACCT ATGAATCTGT GGTTACTGGG TTCTTCCCCT TCTTTCTGGT GGCTACAGTC 
ATCCACCTGT TCTACAGAGG CCGGTTGTGG AACATCTTAC TCTTCTTGTT GACGGTCCAG 
CTGGTGGGTA TGGTGAAGGC CACCTACGCC TGCTTCCTGC GGGGCAGCCT TGTCATGATC 
TTCATGTCAC TCTACTCACT GCTCTACATG TCAAGCCTGC TCCCTGCTAA GATCTTTGCC 
CTGCTGACTA TTAACAAAGC AGGATGGGGC ACATCAGGCA GGAAAAAGAT GGTAGTAAAC 
CTTATTGGAG CTGTGCCTGT TACTGTGTGG ACAGCCATTC TGCTTGGTGG AGTGGTTTAC 
ACCATTTACT GCGAGGTTCA AGAACCTTTT ACCGAGACTG AGAAGGCTCT TCTTATCGCA 
GGCACCATTG TCTATGCCTC TTACTGGCTT ATACTCCTGG TGCTCTACCT GGCCATAGTG 
GCCAAACGTT GTAACAAAAG AGAAGAACAG TTCCACCTTT CCTATGCCGA AGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291796.1
CDS	1595..3271
Translation	MTTMPSRFGTAVRIFITTLFAAVVLFAILLAYVTGYQFIHTEQHHLSFGLYGAFLSLHLLLQSLFAYLEHRQMRGPSRPQHLRRTVALCIAAYQEDPDYLRKCLRSIRRISFPGLKVVLVVDGNRQEDAYMMDIFQEVMGGVEQTGCVVWKGNYHSNGDGGGGGGKGSVHAEEAARVARVVRSCRYSCIMQEWGGKREVMYTAFKALGDTVDYMQVCDSDTVLDPACTIEMLKILEEDPDVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEPWYHQTFLGSKCSFGDDRHLTNRVLSFGFKTKFTARSQCQTETPTQYLRWLNQQTRWSKSYFREWLYNALWFHKHSLWMTYESVVTGFFPFFLVATVIHLFYRGRLWNILLFLLTVQLVGMVKATYACFLRGSLVMIFMSLYSLLYMSSLLPAKIFALLTINKAGWGTSGRKKMVVNLIGAVPVTVWTAILLGGVVYTIYCEVQEPFTETEKALLIAGTIVYASYWLILLVLYLAIVAKRCNKREEQFHLSYAEA

Target ORF information:

RefSeq Version	XM_009293521.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hyaluronan synthase 3 (has3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293521.3

ORF Insert Sequence:

ATGACTACAA TGCCCTCTCG CTTTGGCACT GCGGTGCGGA TCTTCATCAC CACCTTATTT 
GCAGCAGTGG TGCTTTTCGC AATCCTACTA GCCTATGTGA CAGGTTACCA GTTCATCCAC 
ACAGAGCAGC ACCATCTGTC TTTTGGCTTG TACGGTGCAT TTCTATCCCT CCACCTTCTC 
CTGCAGAGTC TCTTCGCCTA CCTGGAGCAC AGACAAATGC GAGGCCCCTC CAGACCGCAG 
CACCTGCGCC GCACTGTGGC CCTCTGCATT GCAGCCTATC AGGAAGATCC AGACTACCTT 
CGCAAGTGTC TGCGGAGCAT TCGCCGCATT TCTTTTCCCG GGCTGAAAGT GGTGTTGGTG 
GTGGATGGCA ATCGGCAGGA GGATGCCTAC ATGATGGATA TCTTCCAGGA GGTGATGGGG 
GGAGTGGAGC AGACAGGCTG TGTGGTGTGG AAAGGGAATT ACCACAGTAA CGGGGATGGA 
GGAGGAGGAG GAGGGAAAGG CTCGGTGCAT GCCGAAGAGG CTGCACGAGT GGCCAGAGTG 
GTGCGGAGCT GCCGTTACTC CTGCATCATG CAGGAATGGG GTGGCAAGAG GGAAGTGATG 
TATACAGCCT TCAAAGCACT TGGAGATACA GTGGATTACA TGCAGGTATG TGATTCAGAC 
ACTGTACTTG ATCCAGCATG CACTATTGAG ATGTTGAAGA TTCTAGAGGA GGATCCTGAT 
GTGGGAGGAG TTGGAGGAGA TGTTCAGATT CTGAACAAGT ATGACTCCTG GATCTCCTTC 
CTGAGCAGTG TCCGCTATTG GATGGCCTTC AATGTGGAGC GGGCCTGCCA GTCTTATTTC 
GGATGTGTGC AGTGCATCAG TGGACCTCTA GGAATGTACC GTAACTCTCT CCTTCAGCAG 
TTCTTAGAGC CCTGGTACCA CCAGACCTTC CTAGGAAGCA AGTGCAGTTT TGGTGATGAT 
CGACATCTCA CCAACCGCGT CCTTAGCTTT GGCTTCAAAA CCAAGTTCAC GGCACGCTCT 
CAATGCCAGA CCGAGACCCC AACACAATAC CTGCGTTGGC TCAATCAGCA GACCCGCTGG 
AGCAAGTCTT ACTTTCGAGA GTGGCTCTAC AATGCTCTTT GGTTCCACAA GCACAGCCTG 
TGGATGACCT ATGAATCTGT GGTTACTGGG TTCTTCCCCT TCTTTCTGGT GGCTACAGTC 
ATCCACCTGT TCTACAGAGG CCGGTTGTGG AACATCTTAC TCTTCTTGTT GACGGTCCAG 
CTGGTGGGTA TGGTGAAGGC CACCTACGCC TGCTTCCTGC GGGGCAGCCT TGTCATGATC 
TTCATGTCAC TCTACTCACT GCTCTACATG TCAAGCCTGC TCCCTGCTAA GATCTTTGCC 
CTGCTGACTA TTAACAAAGC AGGATGGGGC ACATCAGGCA GGAAAAAGAT GGTAGTAAAC 
CTTATTGGAG CTGTGCCTGT TACTGTGTGG ACAGCCATTC TGCTTGGTGG AGTGGTTTAC 
ACCATTTACT GCGAGGTTCA AGAACCTTTT ACCGAGACTG AGAAGGCTCT TCTTATCGCA 
GGCACCATTG TCTATGCCTC TTACTGGCTT ATACTCCTGG TGCTCTACCT GGCCATAGTG 
GCCAAACGTT GTAACAAAAG AGAAGAACAG TTCCACCTTT CCTATGCCGA AGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hyaluronic acid synthesis is required for zebrafish tail fin regeneration.
PloS one12(2)e0171898(2017)
Ouyang X,Panetta NJ,Talbott MD,Payumo AY,Halluin C,Longaker MT,Chen JK

Ancient origin of lubricated joints in bony vertebrates.
eLife5(2016 07)
Askary A,Smeeton J,Paul S,Schindler S,Braasch I,Ellis NA,Postlethwait J,Miller CT,Crump JG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration.
PloS one11(2)e0148202(2016)
Govindan J,Tun KM,Iovine MK

Extracellular component hyaluronic acid and its receptor Hmmr are required for epicardial EMT during heart regeneration.
Cardiovascular research107(4)487-98(2015 Sep)
Missinato MA,Tobita K,Romano N,Carroll JA,Tsang M