neo1a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009303465.3	XP_009301740.1	neogenin isoform X2
XM_009303468.3	XP_009301743.1	neogenin isoform X7
XM_005166588.2	XP_005166645.1	neogenin isoform X4
XM_017356809.1	XP_017212298.1	neogenin isoform X5
XM_009303468.2	XP_009301743.1	neogenin isoform X6
XM_009303469.2	XP_009301744.1	neogenin isoform X7
NM_173218.2	NP_775325.2	neogenin isoform 1 precursor
XM_009303464.2	XP_009301739.1	neogenin isoform X1
XM_009303464.3	XP_009301739.1	neogenin isoform X1
XM_021477387.1	XP_021333062.1	neogenin isoform X5
XM_017356809.2	XP_017212298.1	neogenin isoform X6
NM_001328497.1	NP_001315426.1	neogenin isoform 2 precursor
XM_009303465.2	XP_009301740.1	neogenin isoform X2
XM_009303466.2	XP_009301741.1	neogenin isoform X3
XM_005166588.3	XP_005166645.1	neogenin isoform X4
XM_009303466.3	XP_009301741.1	neogenin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53394	NM_173218.2 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa39593	XM_021477387.1 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa59603	XM_017356809.2 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa39595	XM_009303469.2 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13468	NM_001328497.1 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa13466	XM_005166588.3 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39590	XM_009303464.3 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39591	XM_009303465.3 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39592	XM_009303466.3 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39594	XM_009303468.3 Latest version!	Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa39594	XM_009303468.2	Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39590	XM_009303464.2	Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa59603	XM_017356809.1	Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa39591	XM_009303465.2	Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa13466	XM_005166588.2	Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39592	XM_009303466.2	Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

neo1a ( NM_173218.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775325.2 Danio rerio neogenin 1a (neo1a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa53394
Clone ID Related Accession (Same CDS sequence)	NM_173218.2
Accession Version	NM_173218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-13
Organism	Danio rerio(zebrafish)
Product	neogenin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX927344.10, BX927325.15, CU463832.12 and AL935050.11. On Jun 16, 2016 this sequence version replaced NM_173218.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY029280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GACCCCATAG ACCCTGACGT TGATTTGTAC 
GAACTTTTCC ATGCTCCATA CACTCCAGTA CCAGACCCTA TACCCATGCT GCCTCCTGTG 
GGCGTTCAGG CATCTGTCCT GAACCAAGAC ACCATCAAAG TCACCTGGGC GGACAACTCG 
CTGCCTAAGA ACCAGAAGAT CACAGATGCG CGCTACTATA CAGTGCGCTG GAAGACCAAC 
ATACCCGCAA ACACAAAGTT TAAGGTGGCT AATACCACGA CGCTATTTCA CACGGTGACG 
GGTCTTAAAC CCAATACCTT GTATGAGTTT TCGGTTATGG TGACCAAGGG ACGGAGGACC 
AGTACCTGGA GCATGACTGC ACACGGAACA ACTTTTGAGT CAATCCCAAG TTCTCCACCA 
AAAGATGTGA CTGTAGTGAG CAAAGAGGGA CGACCAAAAA CCATCATCGT CAACTGGCAG 
CCGCCATCAG AAGCCAATGG CAAAATTACA GGTTACATCA TTTATTACAG CACTGATGTG 
AAGGCAGAGG TTCATGACTG GGTCATTGAG CCAGTCGTGG GAAACAGGCT AACCCATCAA 
ATTCAGGAGC TGACCCTTGA TACCACCTAC TACTTTAAGA TCCAGGCTCG GAACTCCAAG 
GGCATGGGTC CCATGTCGGA AGCGGTCCAG TTCCGCACTC CTAAAGCTGA ATCCTCTGAC 
AAAATGGCTA ATGACCAAGC TCCAGGCATA TCTGTGAAAC AAGGAAGACC TTCTGCACCA 
GATAACGGCT TTGGATCACC TGGGAAACCA GACATGTCTG GCACTGGTGA TAACCACATC 
CTGGTTATTG TCATCATTGT GTCCGTGGGT GCTTTTACCA TCATCGTGGT GGTGGTGGGC 
GCCTTCCTTT GTACACGTCG TACCACAACA CAACAGAAAA AGAAAAGGGC CGCCTGCAAG 
TCCGCCAATG GATCCCACAA GTATAAGGGC AACTCCAAAG ATCTCAAACC ACCAGACCTC 
TGGATCCACC ATGAACGTTT GGAGCTAAAG CCCATAGACA AATCACCCGA ACCCAATCCA 
GTCATGACCG ACACACCAAT CCCCAGGACC TCCCAAGACA TCACGCCGGT GGACGGCTCC 
ATGGATCCTA TGCTCCAGAG ACGCAACTCT TACCGTGGCC ATGAATCAGA GGACAGCATG 
TCTACACTGG CAGGCCGTCG GGGGATGAGA CCTAAAATGA TGATGCCCTT TGATGCACAA 
CCCCCTCAGC CTGTGATTAG TGCTCACCCC ATCCATTCCC TCGATAACCA TCACCATCAT 
TATCACTTGG GCAGCCTGGC CTCGCCGACA CGCAGCTATC TCCACCACCA GAGCAGCACG 
CGCTCCATCG CCACCCCCAT CTCTCATCTA GACAGGGCAG AGTCCACAGA GTCAGTAAGG 
AACACCCCTA ATGCGGAGCT GCTCCCTCCA TCTGGGCAGC CATCTTGCAC CACTGACCTT 
TCAGAGACGG ACATTAGCTT TCACAGCTCA GCCGCTGAGG AGGAGCCAGC GTTCGCTACA 
CCCACAGCAC ACGTTCGACC TACACACCCA CTTAAGAGTT TTGCAGTGCC TGCCATCCCG 
GCCCATGCAA TGTATGACTC GGCAGCTTTG CCCAGCACTC CACTGCTGTC TCAGACAGGG 
CCTCACTCAG TTAAAACAGC CTCTATTGGT ACACTGGGAA GGACGAGAAC TCCTATGCCT 
GTCATAGTTC CAAATGCGCC GGATGTCTCA GAAGGCTGCA GACTTCATGA AGATGCAGGA 
AGTAATTACG AGACGGACGA GTTATCGGAG GAAATGGCCC ATCTCGAGGG CTTAATGAAG 
GACCTAAACG CCATCACAAC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775325.2
CDS	402..4688
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	NM_173218.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173218.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GACCCCATAG ACCCTGACGT TGATTTGTAC 
GAACTTTTCC ATGCTCCATA CACTCCAGTA CCAGACCCTA TACCCATGCT GCCTCCTGTG 
GGCGTTCAGG CATCTGTCCT GAACCAAGAC ACCATCAAAG TCACCTGGGC GGACAACTCG 
CTGCCTAAGA ACCAGAAGAT CACAGATGCG CGCTACTATA CAGTGCGCTG GAAGACCAAC 
ATACCCGCAA ACACAAAGTT TAAGGTGGCT AATACCACGA CGCTATTTCA CACGGTGACG 
GGTCTTAAAC CCAATACCTT GTATGAGTTT TCGGTTATGG TGACCAAGGG ACGGAGGACC 
AGTACCTGGA GCATGACTGC ACACGGAACA ACTTTTGAGT CAATCCCAAG TTCTCCACCA 
AAAGATGTGA CTGTAGTGAG CAAAGAGGGA CGACCAAAAA CCATCATCGT CAACTGGCAG 
CCGCCATCAG AAGCCAATGG CAAAATTACA GGTTACATCA TTTATTACAG CACTGATGTG 
AAGGCAGAGG TTCATGACTG GGTCATTGAG CCAGTCGTGG GAAACAGGCT AACCCATCAA 
ATTCAGGAGC TGACCCTTGA TACCACCTAC TACTTTAAGA TCCAGGCTCG GAACTCCAAG 
GGCATGGGTC CCATGTCGGA AGCGGTCCAG TTCCGCACTC CTAAAGCTGA ATCCTCTGAC 
AAAATGGCTA ATGACCAAGC TCCAGGCATA TCTGTGAAAC AAGGAAGACC TTCTGCACCA 
GATAACGGCT TTGGATCACC TGGGAAACCA GACATGTCTG GCACTGGTGA TAACCACATC 
CTGGTTATTG TCATCATTGT GTCCGTGGGT GCTTTTACCA TCATCGTGGT GGTGGTGGGC 
GCCTTCCTTT GTACACGTCG TACCACAACA CAACAGAAAA AGAAAAGGGC CGCCTGCAAG 
TCCGCCAATG GATCCCACAA GTATAAGGGC AACTCCAAAG ATCTCAAACC ACCAGACCTC 
TGGATCCACC ATGAACGTTT GGAGCTAAAG CCCATAGACA AATCACCCGA ACCCAATCCA 
GTCATGACCG ACACACCAAT CCCCAGGACC TCCCAAGACA TCACGCCGGT GGACGGCTCC 
ATGGATCCTA TGCTCCAGAG ACGCAACTCT TACCGTGGCC ATGAATCAGA GGACAGCATG 
TCTACACTGG CAGGCCGTCG GGGGATGAGA CCTAAAATGA TGATGCCCTT TGATGCACAA 
CCCCCTCAGC CTGTGATTAG TGCTCACCCC ATCCATTCCC TCGATAACCA TCACCATCAT 
TATCACTTGG GCAGCCTGGC CTCGCCGACA CGCAGCTATC TCCACCACCA GAGCAGCACG 
CGCTCCATCG CCACCCCCAT CTCTCATCTA GACAGGGCAG AGTCCACAGA GTCAGTAAGG 
AACACCCCTA ATGCGGAGCT GCTCCCTCCA TCTGGGCAGC CATCTTGCAC CACTGACCTT 
TCAGAGACGG ACATTAGCTT TCACAGCTCA GCCGCTGAGG AGGAGCCAGC GTTCGCTACA 
CCCACAGCAC ACGTTCGACC TACACACCCA CTTAAGAGTT TTGCAGTGCC TGCCATCCCG 
GCCCATGCAA TGTATGACTC GGCAGCTTTG CCCAGCACTC CACTGCTGTC TCAGACAGGG 
CCTCACTCAG TTAAAACAGC CTCTATTGGT ACACTGGGAA GGACGAGAAC TCCTATGCCT 
GTCATAGTTC CAAATGCGCC GGATGTCTCA GAAGGCTGCA GACTTCATGA AGATGCAGGA 
AGTAATTACG AGACGGACGA GTTATCGGAG GAAATGGCCC ATCTCGAGGG CTTAATGAAG 
GACCTAAACG CCATCACAAC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_021477387.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333062.1 Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa39593
Clone ID Related Accession (Same CDS sequence)	XM_021477387.1
Accession Version	XM_021477387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTT ACCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333062.1
CDS	403..4680
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSYH

Target ORF information:

RefSeq Version	XM_021477387.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477387.1

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTT ACCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_017356809.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212298.1 Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.

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CloneID	ODa59603
Clone ID Related Accession (Same CDS sequence)	XM_017356809.1 , XM_017356809.2
Accession Version	XM_017356809.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCAGTC AGTAAGGAAC ACCCCTAATG CGGAGCTGCT CCCTCCATCT 
GGGCAGCCAT CTTGCACCAC TGACCTTTCA GAGACGGACA TTAGCTTTCA CAGCTCAGCC 
GCTGAGGAGG AGCCAGCGTT CGCTACACCC ACAGCACACG TTCGACCTAC ACACCCACTT 
AAGAGTTTTG CAGTGCCTGC CATCCCGGCC CATGCAATGT ATGACTCGGC AGCTTTGCCC 
AGCACTCCAC TGCTGTCTCA GACAGGGCCT CACTCAGTTA AAACAGCCTC TATTGGTACA 
CTGGGAAGGA CGAGAACTCC TATGCCTGTC ATAGTTCCAA ATGCGCCGGA TGTCTCAGAA 
GGCTGCAGAC TTCATGAAGA TGCAGGAAGT AATTACGAGA CGGACGAGTT ATCGGAGGAA 
ATGGCCCATC TCGAGGGCTT AATGAAGGAC CTAAACGCCA TCACAACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212298.1
CDS	402..4595
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQQSVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_017356809.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356809.1

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCAGTC AGTAAGGAAC ACCCCTAATG CGGAGCTGCT CCCTCCATCT 
GGGCAGCCAT CTTGCACCAC TGACCTTTCA GAGACGGACA TTAGCTTTCA CAGCTCAGCC 
GCTGAGGAGG AGCCAGCGTT CGCTACACCC ACAGCACACG TTCGACCTAC ACACCCACTT 
AAGAGTTTTG CAGTGCCTGC CATCCCGGCC CATGCAATGT ATGACTCGGC AGCTTTGCCC 
AGCACTCCAC TGCTGTCTCA GACAGGGCCT CACTCAGTTA AAACAGCCTC TATTGGTACA 
CTGGGAAGGA CGAGAACTCC TATGCCTGTC ATAGTTCCAA ATGCGCCGGA TGTCTCAGAA 
GGCTGCAGAC TTCATGAAGA TGCAGGAAGT AATTACGAGA CGGACGAGTT ATCGGAGGAA 
ATGGCCCATC TCGAGGGCTT AATGAAGGAC CTAAACGCCA TCACAACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_017356809.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212298.1 Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.

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CloneID	ODa59603
Clone ID Related Accession (Same CDS sequence)	XM_017356809.1 , XM_017356809.2
Accession Version	XM_017356809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCAGTC AGTAAGGAAC ACCCCTAATG CGGAGCTGCT CCCTCCATCT 
GGGCAGCCAT CTTGCACCAC TGACCTTTCA GAGACGGACA TTAGCTTTCA CAGCTCAGCC 
GCTGAGGAGG AGCCAGCGTT CGCTACACCC ACAGCACACG TTCGACCTAC ACACCCACTT 
AAGAGTTTTG CAGTGCCTGC CATCCCGGCC CATGCAATGT ATGACTCGGC AGCTTTGCCC 
AGCACTCCAC TGCTGTCTCA GACAGGGCCT CACTCAGTTA AAACAGCCTC TATTGGTACA 
CTGGGAAGGA CGAGAACTCC TATGCCTGTC ATAGTTCCAA ATGCGCCGGA TGTCTCAGAA 
GGCTGCAGAC TTCATGAAGA TGCAGGAAGT AATTACGAGA CGGACGAGTT ATCGGAGGAA 
ATGGCCCATC TCGAGGGCTT AATGAAGGAC CTAAACGCCA TCACAACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212298.1
CDS	403..4596
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQQSVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_017356809.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356809.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCAGTC AGTAAGGAAC ACCCCTAATG CGGAGCTGCT CCCTCCATCT 
GGGCAGCCAT CTTGCACCAC TGACCTTTCA GAGACGGACA TTAGCTTTCA CAGCTCAGCC 
GCTGAGGAGG AGCCAGCGTT CGCTACACCC ACAGCACACG TTCGACCTAC ACACCCACTT 
AAGAGTTTTG CAGTGCCTGC CATCCCGGCC CATGCAATGT ATGACTCGGC AGCTTTGCCC 
AGCACTCCAC TGCTGTCTCA GACAGGGCCT CACTCAGTTA AAACAGCCTC TATTGGTACA 
CTGGGAAGGA CGAGAACTCC TATGCCTGTC ATAGTTCCAA ATGCGCCGGA TGTCTCAGAA 
GGCTGCAGAC TTCATGAAGA TGCAGGAAGT AATTACGAGA CGGACGAGTT ATCGGAGGAA 
ATGGCCCATC TCGAGGGCTT AATGAAGGAC CTAAACGCCA TCACAACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303469.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301744.1 Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.

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CloneID	ODa39595
Clone ID Related Accession (Same CDS sequence)	XM_009303469.2
Accession Version	XM_009303469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301744.1
CDS	402..3689
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKK

Target ORF information:

RefSeq Version	XM_009303469.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303469.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( NM_001328497.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315426.1 Danio rerio neogenin 1a (neo1a), transcript variant 2, mRNA.

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CloneID	ODa13468
Clone ID Related Accession (Same CDS sequence)	NM_001328497.1
Accession Version	NM_001328497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-13
Organism	Danio rerio(zebrafish)
Product	neogenin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX927344.10, BX927325.15, CU463832.12 and AL935050.11. On Jun 16, 2016 this sequence version replaced XM_005166590.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01028126.1, AY082380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315426.1
CDS	402..4631
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	NM_001328497.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328497.1

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GGCTCCAGAG GCGGCGCTGG CCCCACGCCC 
TCCGCCCCCC GTGATGTGGT AGCGTCCCTG GTGTCCACCC GCTTCCTCAA GCTGACCTGG 
CGCCTTCCTG CTGAACCACA CGGAGACGAT GTCACCTACT CAGTCTACTA CAGCCTGGAG 
GGCACTAACA GGGAGAGGAT CGTAAACACC AGTCGTCCAG GAGAAATGCA AGTCACCATT 
CAGAACCTCA TGCCAGACAC CAAGTATGCC TTCCGTGTGG TGGCGCACAA CAAAAACGGC 
CCTGGAGAGA GTTCAGTGCC TCTAAAGGTC GAAACTCAGC CCGAAGTCCA GGTACCTGGT 
CCGGCTCCAA ACCTGCATGC AGTGGTCATG TCCCCCAGCA CTGTCAGTCT CAGCTGGGAT 
GTGCCTCTTA TAGGCAATGG TGAGATCCAG AACTACAAGA TCTACTACAT GGAGAAGGGC 
ATGGACAGTG AACAGGATCT GGATGTAAAC ACATTGTCCT ACACCATGAC TGGCCTGAAG 
AAGTTCACGG AGTACAGTTT CAGACTGGTG GCCTATAACA AACATGGACC GGGAGTCTCC 
ACTGAGGACA TATCAGTGCG CACATACTCT GATGTCCCCA GCTCTCCTCC GCAGAACATG 
ACGGTGGAAG TGCTGAACTC CAAGAGCCTC ATGATCAGAT GGCAGCCTCC ACCAGCCGAT 
GCCCAGAACG GAGAGATCAC AGGCTACAAG ATCCGCTACA GGAAGGGTAC GAGGAAGAGC 
GAGGTGGCGG AGATCACTTC AGGATCACAG CTTTATCAGC TCATAGATGG TTTGCAGCGA 
GGCACTGAAT ATATGTTGCG AGTATCTGCA ATGACGGTCA ACGGCACAGG TCCTGCCACA 
GACTGGACCA CTGCAGAGAC ATTCGAAAGC GATCTGGACG AGTCAAGAGT GCCGGACGTC 
CCGAGCTCTC TGCATGTTCG TTCTCTGGTC AACAGTATTG TGGTGAGCTG GACGCCTCCA 
GAGAACCAGG ACATCGTGGT GAGGGGTTAC AGTATCAGCT ATGGGATTGG CAGTCCTCAC 
GCACAGACCA TCAAAGTGGA CTACAAGCAG AGATACTACA CCATTGAGAA CCTGAATCCC 
AACTCGCACT ACGTGATCAC GCTGAAGGCC TTCAACAACG TGGGTGAGGG AATCCCTGTG 
TACGAGAGCG CCATCACACG ACCACAGTCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_005166588.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166645.1 Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.

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CloneID	ODa13466
Clone ID Related Accession (Same CDS sequence)	XM_005166588.2 , XM_005166588.3
Accession Version	XM_005166588.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGTAC CAGACCCTAT ACCCATGCTG 
CCTCCTGTGG GCGTTCAGGC ATCTGTCCTG AACCAAGACA CCATCAAAGT CACCTGGGCG 
GACAACTCGC TGCCTAAGAA CCAGAAGATC ACAGATGCGC GCTACTATAC AGTGCGCTGG 
AAGACCAACA TACCCGCAAA CACAAAGTTT AAGGTGGCTA ATACCACGAC GCTATTTCAC 
ACGGTGACGG GTCTTAAACC CAATACCTTG TATGAGTTTT CGGTTATGGT GACCAAGGGA 
CGGAGGACCA GTACCTGGAG CATGACTGCA CACGGAACAA CTTTTGAGTC AATCCCAAGT 
TCTCCACCAA AAGATGTGAC TGTAGTGAGC AAAGAGGGAC GACCAAAAAC CATCATCGTC 
AACTGGCAGC CGCCATCAGA AGCCAATGGC AAAATTACAG GTTACATCAT TTATTACAGC 
ACTGATGTGA AGGCAGAGGT TCATGACTGG GTCATTGAGC CAGTCGTGGG AAACAGGCTA 
ACCCATCAAA TTCAGGAGCT GACCCTTGAT ACCACCTACT ACTTTAAGAT CCAGGCTCGG 
AACTCCAAGG GCATGGGTCC CATGTCGGAA GCGGTCCAGT TCCGCACTCC TAAAGCTGAA 
TCCTCTGACA AAATGGCTAA TGACCAAGCT CCAGGCATAT CTGTGAAACA AGGAAGACCT 
TCTGCACCAG ATAACGGCTT TGGATCACCT GGGAAACCAG ACATGTCTGG CACTGGTGAT 
AACCACATCC TGGTTATTGT CATCATTGTG TCCGTGGGTG CTTTTACCAT CATCGTGGTG 
GTGGTGGGCG CCTTCCTTTG TACACGTCGT ACCACAACAC AACAGAAAAA GAAAAGGGCC 
GCCTGCAAGT CCGCCAATGG ATCCCACAAG TATAAGGGCA ACTCCAAAGA TCTCAAACCA 
CCAGACCTCT GGATCCACCA TGAACGTTTG GAGCTAAAGC CCATAGACAA ATCACCCGAA 
CCCAATCCAG TCATGACCGA CACACCAATC CCCAGGACCT CCCAAGACAT CACGCCGGTG 
GACGGCTCCA TGGATCCTAT GCTCCAGAGA CGCAACTCTT ACCGTGGCCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGCCGTCGG GGGATGAGAC CTAAAATGAT GATGCCCTTT 
GATGCACAAC CCCCTCAGCC TGTGATTAGT GCTCACCCCA TCCATTCCCT CGATAACCAT 
CACCATCATT ATCACTTGGG CAGCCTGGCC TCGCCGACAC GCAGCTATCT CCACCACCAG 
AGCAGCACGC GCTCCATCGC CACCCCCATC TCTCATCTAG ACAGGGCAGA GTCCACAGAG 
TCAGTAAGGA ACACCCCTAA TGCGGAGCTG CTCCCTCCAT CTGGGCAGCC ATCTTGCACC 
ACTGACCTTT CAGAGACGGA CATTAGCTTT CACAGCTCAG CCGCTGAGGA GGAGCCAGCG 
TTCGCTACAC CCACAGCACA CGTTCGACCT ACACACCCAC TTAAGAGTTT TGCAGTGCCT 
GCCATCCCGG CCCATGCAAT GTATGACTCG GCAGCTTTGC CCAGCACTCC ACTGCTGTCT 
CAGACAGGGC CTCACTCAGT TAAAACAGCC TCTATTGGTA CACTGGGAAG GACGAGAACT 
CCTATGCCTG TCATAGTTCC AAATGCGCCG GATGTCTCAG AAGGCTGCAG ACTTCATGAA 
GATGCAGGAA GTAATTACGA GACGGACGAG TTATCGGAGG AAATGGCCCA TCTCGAGGGC 
TTAATGAAGG ACCTAAACGC CATCACAACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166645.1
CDS	402..4697
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005166588.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166588.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGTAC CAGACCCTAT ACCCATGCTG 
CCTCCTGTGG GCGTTCAGGC ATCTGTCCTG AACCAAGACA CCATCAAAGT CACCTGGGCG 
GACAACTCGC TGCCTAAGAA CCAGAAGATC ACAGATGCGC GCTACTATAC AGTGCGCTGG 
AAGACCAACA TACCCGCAAA CACAAAGTTT AAGGTGGCTA ATACCACGAC GCTATTTCAC 
ACGGTGACGG GTCTTAAACC CAATACCTTG TATGAGTTTT CGGTTATGGT GACCAAGGGA 
CGGAGGACCA GTACCTGGAG CATGACTGCA CACGGAACAA CTTTTGAGTC AATCCCAAGT 
TCTCCACCAA AAGATGTGAC TGTAGTGAGC AAAGAGGGAC GACCAAAAAC CATCATCGTC 
AACTGGCAGC CGCCATCAGA AGCCAATGGC AAAATTACAG GTTACATCAT TTATTACAGC 
ACTGATGTGA AGGCAGAGGT TCATGACTGG GTCATTGAGC CAGTCGTGGG AAACAGGCTA 
ACCCATCAAA TTCAGGAGCT GACCCTTGAT ACCACCTACT ACTTTAAGAT CCAGGCTCGG 
AACTCCAAGG GCATGGGTCC CATGTCGGAA GCGGTCCAGT TCCGCACTCC TAAAGCTGAA 
TCCTCTGACA AAATGGCTAA TGACCAAGCT CCAGGCATAT CTGTGAAACA AGGAAGACCT 
TCTGCACCAG ATAACGGCTT TGGATCACCT GGGAAACCAG ACATGTCTGG CACTGGTGAT 
AACCACATCC TGGTTATTGT CATCATTGTG TCCGTGGGTG CTTTTACCAT CATCGTGGTG 
GTGGTGGGCG CCTTCCTTTG TACACGTCGT ACCACAACAC AACAGAAAAA GAAAAGGGCC 
GCCTGCAAGT CCGCCAATGG ATCCCACAAG TATAAGGGCA ACTCCAAAGA TCTCAAACCA 
CCAGACCTCT GGATCCACCA TGAACGTTTG GAGCTAAAGC CCATAGACAA ATCACCCGAA 
CCCAATCCAG TCATGACCGA CACACCAATC CCCAGGACCT CCCAAGACAT CACGCCGGTG 
GACGGCTCCA TGGATCCTAT GCTCCAGAGA CGCAACTCTT ACCGTGGCCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGCCGTCGG GGGATGAGAC CTAAAATGAT GATGCCCTTT 
GATGCACAAC CCCCTCAGCC TGTGATTAGT GCTCACCCCA TCCATTCCCT CGATAACCAT 
CACCATCATT ATCACTTGGG CAGCCTGGCC TCGCCGACAC GCAGCTATCT CCACCACCAG 
AGCAGCACGC GCTCCATCGC CACCCCCATC TCTCATCTAG ACAGGGCAGA GTCCACAGAG 
TCAGTAAGGA ACACCCCTAA TGCGGAGCTG CTCCCTCCAT CTGGGCAGCC ATCTTGCACC 
ACTGACCTTT CAGAGACGGA CATTAGCTTT CACAGCTCAG CCGCTGAGGA GGAGCCAGCG 
TTCGCTACAC CCACAGCACA CGTTCGACCT ACACACCCAC TTAAGAGTTT TGCAGTGCCT 
GCCATCCCGG CCCATGCAAT GTATGACTCG GCAGCTTTGC CCAGCACTCC ACTGCTGTCT 
CAGACAGGGC CTCACTCAGT TAAAACAGCC TCTATTGGTA CACTGGGAAG GACGAGAACT 
CCTATGCCTG TCATAGTTCC AAATGCGCCG GATGTCTCAG AAGGCTGCAG ACTTCATGAA 
GATGCAGGAA GTAATTACGA GACGGACGAG TTATCGGAGG AAATGGCCCA TCTCGAGGGC 
TTAATGAAGG ACCTAAACGC CATCACAACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_005166588.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166645.1 Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa13466
Clone ID Related Accession (Same CDS sequence)	XM_005166588.2 , XM_005166588.3
Accession Version	XM_005166588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGTAC CAGACCCTAT ACCCATGCTG 
CCTCCTGTGG GCGTTCAGGC ATCTGTCCTG AACCAAGACA CCATCAAAGT CACCTGGGCG 
GACAACTCGC TGCCTAAGAA CCAGAAGATC ACAGATGCGC GCTACTATAC AGTGCGCTGG 
AAGACCAACA TACCCGCAAA CACAAAGTTT AAGGTGGCTA ATACCACGAC GCTATTTCAC 
ACGGTGACGG GTCTTAAACC CAATACCTTG TATGAGTTTT CGGTTATGGT GACCAAGGGA 
CGGAGGACCA GTACCTGGAG CATGACTGCA CACGGAACAA CTTTTGAGTC AATCCCAAGT 
TCTCCACCAA AAGATGTGAC TGTAGTGAGC AAAGAGGGAC GACCAAAAAC CATCATCGTC 
AACTGGCAGC CGCCATCAGA AGCCAATGGC AAAATTACAG GTTACATCAT TTATTACAGC 
ACTGATGTGA AGGCAGAGGT TCATGACTGG GTCATTGAGC CAGTCGTGGG AAACAGGCTA 
ACCCATCAAA TTCAGGAGCT GACCCTTGAT ACCACCTACT ACTTTAAGAT CCAGGCTCGG 
AACTCCAAGG GCATGGGTCC CATGTCGGAA GCGGTCCAGT TCCGCACTCC TAAAGCTGAA 
TCCTCTGACA AAATGGCTAA TGACCAAGCT CCAGGCATAT CTGTGAAACA AGGAAGACCT 
TCTGCACCAG ATAACGGCTT TGGATCACCT GGGAAACCAG ACATGTCTGG CACTGGTGAT 
AACCACATCC TGGTTATTGT CATCATTGTG TCCGTGGGTG CTTTTACCAT CATCGTGGTG 
GTGGTGGGCG CCTTCCTTTG TACACGTCGT ACCACAACAC AACAGAAAAA GAAAAGGGCC 
GCCTGCAAGT CCGCCAATGG ATCCCACAAG TATAAGGGCA ACTCCAAAGA TCTCAAACCA 
CCAGACCTCT GGATCCACCA TGAACGTTTG GAGCTAAAGC CCATAGACAA ATCACCCGAA 
CCCAATCCAG TCATGACCGA CACACCAATC CCCAGGACCT CCCAAGACAT CACGCCGGTG 
GACGGCTCCA TGGATCCTAT GCTCCAGAGA CGCAACTCTT ACCGTGGCCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGCCGTCGG GGGATGAGAC CTAAAATGAT GATGCCCTTT 
GATGCACAAC CCCCTCAGCC TGTGATTAGT GCTCACCCCA TCCATTCCCT CGATAACCAT 
CACCATCATT ATCACTTGGG CAGCCTGGCC TCGCCGACAC GCAGCTATCT CCACCACCAG 
AGCAGCACGC GCTCCATCGC CACCCCCATC TCTCATCTAG ACAGGGCAGA GTCCACAGAG 
TCAGTAAGGA ACACCCCTAA TGCGGAGCTG CTCCCTCCAT CTGGGCAGCC ATCTTGCACC 
ACTGACCTTT CAGAGACGGA CATTAGCTTT CACAGCTCAG CCGCTGAGGA GGAGCCAGCG 
TTCGCTACAC CCACAGCACA CGTTCGACCT ACACACCCAC TTAAGAGTTT TGCAGTGCCT 
GCCATCCCGG CCCATGCAAT GTATGACTCG GCAGCTTTGC CCAGCACTCC ACTGCTGTCT 
CAGACAGGGC CTCACTCAGT TAAAACAGCC TCTATTGGTA CACTGGGAAG GACGAGAACT 
CCTATGCCTG TCATAGTTCC AAATGCGCCG GATGTCTCAG AAGGCTGCAG ACTTCATGAA 
GATGCAGGAA GTAATTACGA GACGGACGAG TTATCGGAGG AAATGGCCCA TCTCGAGGGC 
TTAATGAAGG ACCTAAACGC CATCACAACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166645.1
CDS	402..4697
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005166588.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166588.3

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGTAC CAGACCCTAT ACCCATGCTG 
CCTCCTGTGG GCGTTCAGGC ATCTGTCCTG AACCAAGACA CCATCAAAGT CACCTGGGCG 
GACAACTCGC TGCCTAAGAA CCAGAAGATC ACAGATGCGC GCTACTATAC AGTGCGCTGG 
AAGACCAACA TACCCGCAAA CACAAAGTTT AAGGTGGCTA ATACCACGAC GCTATTTCAC 
ACGGTGACGG GTCTTAAACC CAATACCTTG TATGAGTTTT CGGTTATGGT GACCAAGGGA 
CGGAGGACCA GTACCTGGAG CATGACTGCA CACGGAACAA CTTTTGAGTC AATCCCAAGT 
TCTCCACCAA AAGATGTGAC TGTAGTGAGC AAAGAGGGAC GACCAAAAAC CATCATCGTC 
AACTGGCAGC CGCCATCAGA AGCCAATGGC AAAATTACAG GTTACATCAT TTATTACAGC 
ACTGATGTGA AGGCAGAGGT TCATGACTGG GTCATTGAGC CAGTCGTGGG AAACAGGCTA 
ACCCATCAAA TTCAGGAGCT GACCCTTGAT ACCACCTACT ACTTTAAGAT CCAGGCTCGG 
AACTCCAAGG GCATGGGTCC CATGTCGGAA GCGGTCCAGT TCCGCACTCC TAAAGCTGAA 
TCCTCTGACA AAATGGCTAA TGACCAAGCT CCAGGCATAT CTGTGAAACA AGGAAGACCT 
TCTGCACCAG ATAACGGCTT TGGATCACCT GGGAAACCAG ACATGTCTGG CACTGGTGAT 
AACCACATCC TGGTTATTGT CATCATTGTG TCCGTGGGTG CTTTTACCAT CATCGTGGTG 
GTGGTGGGCG CCTTCCTTTG TACACGTCGT ACCACAACAC AACAGAAAAA GAAAAGGGCC 
GCCTGCAAGT CCGCCAATGG ATCCCACAAG TATAAGGGCA ACTCCAAAGA TCTCAAACCA 
CCAGACCTCT GGATCCACCA TGAACGTTTG GAGCTAAAGC CCATAGACAA ATCACCCGAA 
CCCAATCCAG TCATGACCGA CACACCAATC CCCAGGACCT CCCAAGACAT CACGCCGGTG 
GACGGCTCCA TGGATCCTAT GCTCCAGAGA CGCAACTCTT ACCGTGGCCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGCCGTCGG GGGATGAGAC CTAAAATGAT GATGCCCTTT 
GATGCACAAC CCCCTCAGCC TGTGATTAGT GCTCACCCCA TCCATTCCCT CGATAACCAT 
CACCATCATT ATCACTTGGG CAGCCTGGCC TCGCCGACAC GCAGCTATCT CCACCACCAG 
AGCAGCACGC GCTCCATCGC CACCCCCATC TCTCATCTAG ACAGGGCAGA GTCCACAGAG 
TCAGTAAGGA ACACCCCTAA TGCGGAGCTG CTCCCTCCAT CTGGGCAGCC ATCTTGCACC 
ACTGACCTTT CAGAGACGGA CATTAGCTTT CACAGCTCAG CCGCTGAGGA GGAGCCAGCG 
TTCGCTACAC CCACAGCACA CGTTCGACCT ACACACCCAC TTAAGAGTTT TGCAGTGCCT 
GCCATCCCGG CCCATGCAAT GTATGACTCG GCAGCTTTGC CCAGCACTCC ACTGCTGTCT 
CAGACAGGGC CTCACTCAGT TAAAACAGCC TCTATTGGTA CACTGGGAAG GACGAGAACT 
CCTATGCCTG TCATAGTTCC AAATGCGCCG GATGTCTCAG AAGGCTGCAG ACTTCATGAA 
GATGCAGGAA GTAATTACGA GACGGACGAG TTATCGGAGG AAATGGCCCA TCTCGAGGGC 
TTAATGAAGG ACCTAAACGC CATCACAACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303464.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301739.1 Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39590
Clone ID Related Accession (Same CDS sequence)	XM_009303464.2 , XM_009303464.3
Accession Version	XM_009303464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTA ATTACGAGAC GGACGAGTTA TCGGAGGAAA TGGCCCATCT CGAGGGCTTA 
ATGAAGGACC TAAACGCCAT CACAACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301739.1
CDS	402..4754
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303464.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303464.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTA ATTACGAGAC GGACGAGTTA TCGGAGGAAA TGGCCCATCT CGAGGGCTTA 
ATGAAGGACC TAAACGCCAT CACAACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303464.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301739.1 Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.

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CloneID	ODa39590
Clone ID Related Accession (Same CDS sequence)	XM_009303464.2 , XM_009303464.3
Accession Version	XM_009303464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTA ATTACGAGAC GGACGAGTTA TCGGAGGAAA TGGCCCATCT CGAGGGCTTA 
ATGAAGGACC TAAACGCCAT CACAACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301739.1
CDS	402..4754
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303464.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303464.3

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGGCCATGA ATCAGAGGAC 
AGCATGTCTA CACTGGCAGG CCGTCGGGGG ATGAGACCTA AAATGATGAT GCCCTTTGAT 
GCACAACCCC CTCAGCCTGT GATTAGTGCT CACCCCATCC ATTCCCTCGA TAACCATCAC 
CATCATTATC ACTTGGGCAG CCTGGCCTCG CCGACACGCA GCTATCTCCA CCACCAGAGC 
AGCACGCGCT CCATCGCCAC CCCCATCTCT CATCTAGACA GGGCAGAGTC CACAGAGTCA 
GTAAGGAACA CCCCTAATGC GGAGCTGCTC CCTCCATCTG GGCAGCCATC TTGCACCACT 
GACCTTTCAG AGACGGACAT TAGCTTTCAC AGCTCAGCCG CTGAGGAGGA GCCAGCGTTC 
GCTACACCCA CAGCACACGT TCGACCTACA CACCCACTTA AGAGTTTTGC AGTGCCTGCC 
ATCCCGGCCC ATGCAATGTA TGACTCGGCA GCTTTGCCCA GCACTCCACT GCTGTCTCAG 
ACAGGGCCTC ACTCAGTTAA AACAGCCTCT ATTGGTACAC TGGGAAGGAC GAGAACTCCT 
ATGCCTGTCA TAGTTCCAAA TGCGCCGGAT GTCTCAGAAG GCTGCAGACT TCATGAAGAT 
GCAGGAAGTA ATTACGAGAC GGACGAGTTA TCGGAGGAAA TGGCCCATCT CGAGGGCTTA 
ATGAAGGACC TAAACGCCAT CACAACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303465.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301740.1 Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.

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CloneID	ODa39591
Clone ID Related Accession (Same CDS sequence)	XM_009303465.3 , XM_009303465.2
Accession Version	XM_009303465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGTG GCCGCTTTGA GATACTCGGC 
GGGGGGAGTC TGCGGATCTT CAATCTCACA GAGGAGGATG CCGGGGTGTA CAACTGTCTC 
GCAGAAAACA CCAATGGGTC CATTGAGGCC CAGGCTGAGC TCACGCTGAA GGATTCTCCA 
CAGTTCCTGA AGAAACCTGT GAATGTGTTT GCTCATGAAG CCACAGACGT CATCTTCGAG 
TGCGAGGTCT CAGGATCACC TTCTCCCACC ATCAAATGGG TGAAAAATGG AGACGCCGTC 
ATCCCAAGCG ATTATTTCAA AATCATTAAG GAGCAGAACC TGCAGGTGTT GGGACTGGTC 
AAGTCAGATG AAGGTTTTTA TCAGTGTTTA GCTGAGAATG ACGCTGGCAA CGTGCAGTCA 
AGTGCTCAGC TCGTCATATT GGATCAAGAT GTAACCCTCC CCTTACCCCG TCCCACCTCA 
CTGACCCGTG CTACTACTGA CCGTCTAATG CCAGGCTCCA GAGGCGGCGC TGGCCCCACG 
CCCTCCGCCC CCCGTGATGT GGTAGCGTCC CTGGTGTCCA CCCGCTTCCT CAAGCTGACC 
TGGCGCCTTC CTGCTGAACC ACACGGAGAC GATGTCACCT ACTCAGTCTA CTACAGCCTG 
GAGGGCACTA ACAGGGAGAG GATCGTAAAC ACCAGTCGTC CAGGAGAAAT GCAAGTCACC 
ATTCAGAACC TCATGCCAGA CACCAAGTAT GCCTTCCGTG TGGTGGCGCA CAACAAAAAC 
GGCCCTGGAG AGAGTTCAGT GCCTCTAAAG GTCGAAACTC AGCCCGAAGT CCAGGTACCT 
GGTCCGGCTC CAAACCTGCA TGCAGTGGTC ATGTCCCCCA GCACTGTCAG TCTCAGCTGG 
GATGTGCCTC TTATAGGCAA TGGTGAGATC CAGAACTACA AGATCTACTA CATGGAGAAG 
GGCATGGACA GTGAACAGGA TCTGGATGTA AACACATTGT CCTACACCAT GACTGGCCTG 
AAGAAGTTCA CGGAGTACAG TTTCAGACTG GTGGCCTATA ACAAACATGG ACCGGGAGTC 
TCCACTGAGG ACATATCAGT GCGCACATAC TCTGATGTCC CCAGCTCTCC TCCGCAGAAC 
ATGACGGTGG AAGTGCTGAA CTCCAAGAGC CTCATGATCA GATGGCAGCC TCCACCAGCC 
GATGCCCAGA ACGGAGAGAT CACAGGCTAC AAGATCCGCT ACAGGAAGGG TACGAGGAAG 
AGCGAGGTGG CGGAGATCAC TTCAGGATCA CAGCTTTATC AGCTCATAGA TGGTTTGCAG 
CGAGGCACTG AATATATGTT GCGAGTATCT GCAATGACGG TCAACGGCAC AGGTCCTGCC 
ACAGACTGGA CCACTGCAGA GACATTCGAA AGCGATCTGG ACGAGTCAAG AGTGCCGGAC 
GTCCCGAGCT CTCTGCATGT TCGTTCTCTG GTCAACAGTA TTGTGGTGAG CTGGACGCCT 
CCAGAGAACC AGGACATCGT GGTGAGGGGT TACAGTATCA GCTATGGGAT TGGCAGTCCT 
CACGCACAGA CCATCAAAGT GGACTACAAG CAGAGATACT ACACCATTGA GAACCTGAAT 
CCCAACTCGC ACTACGTGAT CACGCTGAAG GCCTTCAACA ACGTGGGTGA GGGAATCCCT 
GTGTACGAGA GCGCCATCAC ACGACCACAG TCAGACCCCA TAGACCCTGA CGTTGATTTG 
TACGAACTTT TCCATGCTCC ATACACTCCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301740.1
CDS	403..4752
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303465.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303465.3

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGTG GCCGCTTTGA GATACTCGGC 
GGGGGGAGTC TGCGGATCTT CAATCTCACA GAGGAGGATG CCGGGGTGTA CAACTGTCTC 
GCAGAAAACA CCAATGGGTC CATTGAGGCC CAGGCTGAGC TCACGCTGAA GGATTCTCCA 
CAGTTCCTGA AGAAACCTGT GAATGTGTTT GCTCATGAAG CCACAGACGT CATCTTCGAG 
TGCGAGGTCT CAGGATCACC TTCTCCCACC ATCAAATGGG TGAAAAATGG AGACGCCGTC 
ATCCCAAGCG ATTATTTCAA AATCATTAAG GAGCAGAACC TGCAGGTGTT GGGACTGGTC 
AAGTCAGATG AAGGTTTTTA TCAGTGTTTA GCTGAGAATG ACGCTGGCAA CGTGCAGTCA 
AGTGCTCAGC TCGTCATATT GGATCAAGAT GTAACCCTCC CCTTACCCCG TCCCACCTCA 
CTGACCCGTG CTACTACTGA CCGTCTAATG CCAGGCTCCA GAGGCGGCGC TGGCCCCACG 
CCCTCCGCCC CCCGTGATGT GGTAGCGTCC CTGGTGTCCA CCCGCTTCCT CAAGCTGACC 
TGGCGCCTTC CTGCTGAACC ACACGGAGAC GATGTCACCT ACTCAGTCTA CTACAGCCTG 
GAGGGCACTA ACAGGGAGAG GATCGTAAAC ACCAGTCGTC CAGGAGAAAT GCAAGTCACC 
ATTCAGAACC TCATGCCAGA CACCAAGTAT GCCTTCCGTG TGGTGGCGCA CAACAAAAAC 
GGCCCTGGAG AGAGTTCAGT GCCTCTAAAG GTCGAAACTC AGCCCGAAGT CCAGGTACCT 
GGTCCGGCTC CAAACCTGCA TGCAGTGGTC ATGTCCCCCA GCACTGTCAG TCTCAGCTGG 
GATGTGCCTC TTATAGGCAA TGGTGAGATC CAGAACTACA AGATCTACTA CATGGAGAAG 
GGCATGGACA GTGAACAGGA TCTGGATGTA AACACATTGT CCTACACCAT GACTGGCCTG 
AAGAAGTTCA CGGAGTACAG TTTCAGACTG GTGGCCTATA ACAAACATGG ACCGGGAGTC 
TCCACTGAGG ACATATCAGT GCGCACATAC TCTGATGTCC CCAGCTCTCC TCCGCAGAAC 
ATGACGGTGG AAGTGCTGAA CTCCAAGAGC CTCATGATCA GATGGCAGCC TCCACCAGCC 
GATGCCCAGA ACGGAGAGAT CACAGGCTAC AAGATCCGCT ACAGGAAGGG TACGAGGAAG 
AGCGAGGTGG CGGAGATCAC TTCAGGATCA CAGCTTTATC AGCTCATAGA TGGTTTGCAG 
CGAGGCACTG AATATATGTT GCGAGTATCT GCAATGACGG TCAACGGCAC AGGTCCTGCC 
ACAGACTGGA CCACTGCAGA GACATTCGAA AGCGATCTGG ACGAGTCAAG AGTGCCGGAC 
GTCCCGAGCT CTCTGCATGT TCGTTCTCTG GTCAACAGTA TTGTGGTGAG CTGGACGCCT 
CCAGAGAACC AGGACATCGT GGTGAGGGGT TACAGTATCA GCTATGGGAT TGGCAGTCCT 
CACGCACAGA CCATCAAAGT GGACTACAAG CAGAGATACT ACACCATTGA GAACCTGAAT 
CCCAACTCGC ACTACGTGAT CACGCTGAAG GCCTTCAACA ACGTGGGTGA GGGAATCCCT 
GTGTACGAGA GCGCCATCAC ACGACCACAG TCAGACCCCA TAGACCCTGA CGTTGATTTG 
TACGAACTTT TCCATGCTCC ATACACTCCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303465.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301740.1 Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39591
Clone ID Related Accession (Same CDS sequence)	XM_009303465.3 , XM_009303465.2
Accession Version	XM_009303465.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGTG GCCGCTTTGA GATACTCGGC 
GGGGGGAGTC TGCGGATCTT CAATCTCACA GAGGAGGATG CCGGGGTGTA CAACTGTCTC 
GCAGAAAACA CCAATGGGTC CATTGAGGCC CAGGCTGAGC TCACGCTGAA GGATTCTCCA 
CAGTTCCTGA AGAAACCTGT GAATGTGTTT GCTCATGAAG CCACAGACGT CATCTTCGAG 
TGCGAGGTCT CAGGATCACC TTCTCCCACC ATCAAATGGG TGAAAAATGG AGACGCCGTC 
ATCCCAAGCG ATTATTTCAA AATCATTAAG GAGCAGAACC TGCAGGTGTT GGGACTGGTC 
AAGTCAGATG AAGGTTTTTA TCAGTGTTTA GCTGAGAATG ACGCTGGCAA CGTGCAGTCA 
AGTGCTCAGC TCGTCATATT GGATCAAGAT GTAACCCTCC CCTTACCCCG TCCCACCTCA 
CTGACCCGTG CTACTACTGA CCGTCTAATG CCAGGCTCCA GAGGCGGCGC TGGCCCCACG 
CCCTCCGCCC CCCGTGATGT GGTAGCGTCC CTGGTGTCCA CCCGCTTCCT CAAGCTGACC 
TGGCGCCTTC CTGCTGAACC ACACGGAGAC GATGTCACCT ACTCAGTCTA CTACAGCCTG 
GAGGGCACTA ACAGGGAGAG GATCGTAAAC ACCAGTCGTC CAGGAGAAAT GCAAGTCACC 
ATTCAGAACC TCATGCCAGA CACCAAGTAT GCCTTCCGTG TGGTGGCGCA CAACAAAAAC 
GGCCCTGGAG AGAGTTCAGT GCCTCTAAAG GTCGAAACTC AGCCCGAAGT CCAGGTACCT 
GGTCCGGCTC CAAACCTGCA TGCAGTGGTC ATGTCCCCCA GCACTGTCAG TCTCAGCTGG 
GATGTGCCTC TTATAGGCAA TGGTGAGATC CAGAACTACA AGATCTACTA CATGGAGAAG 
GGCATGGACA GTGAACAGGA TCTGGATGTA AACACATTGT CCTACACCAT GACTGGCCTG 
AAGAAGTTCA CGGAGTACAG TTTCAGACTG GTGGCCTATA ACAAACATGG ACCGGGAGTC 
TCCACTGAGG ACATATCAGT GCGCACATAC TCTGATGTCC CCAGCTCTCC TCCGCAGAAC 
ATGACGGTGG AAGTGCTGAA CTCCAAGAGC CTCATGATCA GATGGCAGCC TCCACCAGCC 
GATGCCCAGA ACGGAGAGAT CACAGGCTAC AAGATCCGCT ACAGGAAGGG TACGAGGAAG 
AGCGAGGTGG CGGAGATCAC TTCAGGATCA CAGCTTTATC AGCTCATAGA TGGTTTGCAG 
CGAGGCACTG AATATATGTT GCGAGTATCT GCAATGACGG TCAACGGCAC AGGTCCTGCC 
ACAGACTGGA CCACTGCAGA GACATTCGAA AGCGATCTGG ACGAGTCAAG AGTGCCGGAC 
GTCCCGAGCT CTCTGCATGT TCGTTCTCTG GTCAACAGTA TTGTGGTGAG CTGGACGCCT 
CCAGAGAACC AGGACATCGT GGTGAGGGGT TACAGTATCA GCTATGGGAT TGGCAGTCCT 
CACGCACAGA CCATCAAAGT GGACTACAAG CAGAGATACT ACACCATTGA GAACCTGAAT 
CCCAACTCGC ACTACGTGAT CACGCTGAAG GCCTTCAACA ACGTGGGTGA GGGAATCCCT 
GTGTACGAGA GCGCCATCAC ACGACCACAG TCAGACCCCA TAGACCCTGA CGTTGATTTG 
TACGAACTTT TCCATGCTCC ATACACTCCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301740.1
CDS	402..4751
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303465.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303465.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGTG GCCGCTTTGA GATACTCGGC 
GGGGGGAGTC TGCGGATCTT CAATCTCACA GAGGAGGATG CCGGGGTGTA CAACTGTCTC 
GCAGAAAACA CCAATGGGTC CATTGAGGCC CAGGCTGAGC TCACGCTGAA GGATTCTCCA 
CAGTTCCTGA AGAAACCTGT GAATGTGTTT GCTCATGAAG CCACAGACGT CATCTTCGAG 
TGCGAGGTCT CAGGATCACC TTCTCCCACC ATCAAATGGG TGAAAAATGG AGACGCCGTC 
ATCCCAAGCG ATTATTTCAA AATCATTAAG GAGCAGAACC TGCAGGTGTT GGGACTGGTC 
AAGTCAGATG AAGGTTTTTA TCAGTGTTTA GCTGAGAATG ACGCTGGCAA CGTGCAGTCA 
AGTGCTCAGC TCGTCATATT GGATCAAGAT GTAACCCTCC CCTTACCCCG TCCCACCTCA 
CTGACCCGTG CTACTACTGA CCGTCTAATG CCAGGCTCCA GAGGCGGCGC TGGCCCCACG 
CCCTCCGCCC CCCGTGATGT GGTAGCGTCC CTGGTGTCCA CCCGCTTCCT CAAGCTGACC 
TGGCGCCTTC CTGCTGAACC ACACGGAGAC GATGTCACCT ACTCAGTCTA CTACAGCCTG 
GAGGGCACTA ACAGGGAGAG GATCGTAAAC ACCAGTCGTC CAGGAGAAAT GCAAGTCACC 
ATTCAGAACC TCATGCCAGA CACCAAGTAT GCCTTCCGTG TGGTGGCGCA CAACAAAAAC 
GGCCCTGGAG AGAGTTCAGT GCCTCTAAAG GTCGAAACTC AGCCCGAAGT CCAGGTACCT 
GGTCCGGCTC CAAACCTGCA TGCAGTGGTC ATGTCCCCCA GCACTGTCAG TCTCAGCTGG 
GATGTGCCTC TTATAGGCAA TGGTGAGATC CAGAACTACA AGATCTACTA CATGGAGAAG 
GGCATGGACA GTGAACAGGA TCTGGATGTA AACACATTGT CCTACACCAT GACTGGCCTG 
AAGAAGTTCA CGGAGTACAG TTTCAGACTG GTGGCCTATA ACAAACATGG ACCGGGAGTC 
TCCACTGAGG ACATATCAGT GCGCACATAC TCTGATGTCC CCAGCTCTCC TCCGCAGAAC 
ATGACGGTGG AAGTGCTGAA CTCCAAGAGC CTCATGATCA GATGGCAGCC TCCACCAGCC 
GATGCCCAGA ACGGAGAGAT CACAGGCTAC AAGATCCGCT ACAGGAAGGG TACGAGGAAG 
AGCGAGGTGG CGGAGATCAC TTCAGGATCA CAGCTTTATC AGCTCATAGA TGGTTTGCAG 
CGAGGCACTG AATATATGTT GCGAGTATCT GCAATGACGG TCAACGGCAC AGGTCCTGCC 
ACAGACTGGA CCACTGCAGA GACATTCGAA AGCGATCTGG ACGAGTCAAG AGTGCCGGAC 
GTCCCGAGCT CTCTGCATGT TCGTTCTCTG GTCAACAGTA TTGTGGTGAG CTGGACGCCT 
CCAGAGAACC AGGACATCGT GGTGAGGGGT TACAGTATCA GCTATGGGAT TGGCAGTCCT 
CACGCACAGA CCATCAAAGT GGACTACAAG CAGAGATACT ACACCATTGA GAACCTGAAT 
CCCAACTCGC ACTACGTGAT CACGCTGAAG GCCTTCAACA ACGTGGGTGA GGGAATCCCT 
GTGTACGAGA GCGCCATCAC ACGACCACAG TCAGACCCCA TAGACCCTGA CGTTGATTTG 
TACGAACTTT TCCATGCTCC ATACACTCCA GTACCAGACC CTATACCCAT GCTGCCTCCT 
GTGGGCGTTC AGGCATCTGT CCTGAACCAA GACACCATCA AAGTCACCTG GGCGGACAAC 
TCGCTGCCTA AGAACCAGAA GATCACAGAT GCGCGCTACT ATACAGTGCG CTGGAAGACC 
AACATACCCG CAAACACAAA GTTTAAGGTG GCTAATACCA CGACGCTATT TCACACGGTG 
ACGGGTCTTA AACCCAATAC CTTGTATGAG TTTTCGGTTA TGGTGACCAA GGGACGGAGG 
ACCAGTACCT GGAGCATGAC TGCACACGGA ACAACTTTTG AGTCAATCCC AAGTTCTCCA 
CCAAAAGATG TGACTGTAGT GAGCAAAGAG GGACGACCAA AAACCATCAT CGTCAACTGG 
CAGCCGCCAT CAGAAGCCAA TGGCAAAATT ACAGGTTACA TCATTTATTA CAGCACTGAT 
GTGAAGGCAG AGGTTCATGA CTGGGTCATT GAGCCAGTCG TGGGAAACAG GCTAACCCAT 
CAAATTCAGG AGCTGACCCT TGATACCACC TACTACTTTA AGATCCAGGC TCGGAACTCC 
AAGGGCATGG GTCCCATGTC GGAAGCGGTC CAGTTCCGCA CTCCTAAAGC TGAATCCTCT 
GACAAAATGG CTAATGACCA AGCTCCAGGC ATATCTGTGA AACAAGGAAG ACCTTCTGCA 
CCAGATAACG GCTTTGGATC ACCTGGGAAA CCAGACATGT CTGGCACTGG TGATAACCAC 
ATCCTGGTTA TTGTCATCAT TGTGTCCGTG GGTGCTTTTA CCATCATCGT GGTGGTGGTG 
GGCGCCTTCC TTTGTACACG TCGTACCACA ACACAACAGA AAAAGAAAAG GGCCGCCTGC 
AAGTCCGCCA ATGGATCCCA CAAGTATAAG GGCAACTCCA AAGATCTCAA ACCACCAGAC 
CTCTGGATCC ACCATGAACG TTTGGAGCTA AAGCCCATAG ACAAATCACC CGAACCCAAT 
CCAGTCATGA CCGACACACC AATCCCCAGG ACCTCCCAAG ACATCACGCC GGTGGACGGC 
TCCATGGATC CTATGCTCCA GAGACGCAAC TCTTACCGTG GCCATGAATC AGAGGACAGC 
ATGTCTACAC TGGCAGGCCG TCGGGGGATG AGACCTAAAA TGATGATGCC CTTTGATGCA 
CAACCCCCTC AGCCTGTGAT TAGTGCTCAC CCCATCCATT CCCTCGATAA CCATCACCAT 
CATTATCACT TGGGCAGCCT GGCCTCGCCG ACACGCAGCT ATCTCCACCA CCAGAGCAGC 
ACGCGCTCCA TCGCCACCCC CATCTCTCAT CTAGACAGGG CAGAGTCCAC AGAGTCAGTA 
AGGAACACCC CTAATGCGGA GCTGCTCCCT CCATCTGGGC AGCCATCTTG CACCACTGAC 
CTTTCAGAGA CGGACATTAG CTTTCACAGC TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT 
ACACCCACAG CACACGTTCG ACCTACACAC CCACTTAAGA GTTTTGCAGT GCCTGCCATC 
CCGGCCCATG CAATGTATGA CTCGGCAGCT TTGCCCAGCA CTCCACTGCT GTCTCAGACA 
GGGCCTCACT CAGTTAAAAC AGCCTCTATT GGTACACTGG GAAGGACGAG AACTCCTATG 
CCTGTCATAG TTCCAAATGC GCCGGATGTC TCAGAAGGCT GCAGACTTCA TGAAGATGCA 
GGAAGTAATT ACGAGACGGA CGAGTTATCG GAGGAAATGG CCCATCTCGA GGGCTTAATG 
AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303466.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301741.1 Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39592
Clone ID Related Accession (Same CDS sequence)	XM_009303466.2 , XM_009303466.3
Accession Version	XM_009303466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTCCAGGC 
ATATCTGTGA AACAAGGAAG ACCTTCTGCA CCAGATAACG GCTTTGGATC ACCTGGGAAA 
CCAGACATGT CTGGCACTGG TGATAACCAC ATCCTGGTTA TTGTCATCAT TGTGTCCGTG 
GGTGCTTTTA CCATCATCGT GGTGGTGGTG GGCGCCTTCC TTTGTACACG TCGTACCACA 
ACACAACAGA AAAAGAAAAG GGCCGCCTGC AAGTCCGCCA ATGGATCCCA CAAGTATAAG 
GGCAACTCCA AAGATCTCAA ACCACCAGAC CTCTGGATCC ACCATGAACG TTTGGAGCTA 
AAGCCCATAG ACAAATCACC CGAACCCAAT CCAGTCATGA CCGACACACC AATCCCCAGG 
ACCTCCCAAG ACATCACGCC GGTGGACGGC TCCATGGATC CTATGCTCCA GAGACGCAAC 
TCTTACCGTG GCCATGAATC AGAGGACAGC ATGTCTACAC TGGCAGGCCG TCGGGGGATG 
AGACCTAAAA TGATGATGCC CTTTGATGCA CAACCCCCTC AGCCTGTGAT TAGTGCTCAC 
CCCATCCATT CCCTCGATAA CCATCACCAT CATTATCACT TGGGCAGCCT GGCCTCGCCG 
ACACGCAGCT ATCTCCACCA CCAGAGCAGC ACGCGCTCCA TCGCCACCCC CATCTCTCAT 
CTAGACAGGG CAGAGTCCAC AGAGTCAGTA AGGAACACCC CTAATGCGGA GCTGCTCCCT 
CCATCTGGGC AGCCATCTTG CACCACTGAC CTTTCAGAGA CGGACATTAG CTTTCACAGC 
TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT ACACCCACAG CACACGTTCG ACCTACACAC 
CCACTTAAGA GTTTTGCAGT GCCTGCCATC CCGGCCCATG CAATGTATGA CTCGGCAGCT 
TTGCCCAGCA CTCCACTGCT GTCTCAGACA GGGCCTCACT CAGTTAAAAC AGCCTCTATT 
GGTACACTGG GAAGGACGAG AACTCCTATG CCTGTCATAG TTCCAAATGC GCCGGATGTC 
TCAGAAGGCT GCAGACTTCA TGAAGATGCA GGAAGTAATT ACGAGACGGA CGAGTTATCG 
GAGGAAATGG CCCATCTCGA GGGCTTAATG AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301741.1
CDS	402..4721
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303466.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303466.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTCCAGGC 
ATATCTGTGA AACAAGGAAG ACCTTCTGCA CCAGATAACG GCTTTGGATC ACCTGGGAAA 
CCAGACATGT CTGGCACTGG TGATAACCAC ATCCTGGTTA TTGTCATCAT TGTGTCCGTG 
GGTGCTTTTA CCATCATCGT GGTGGTGGTG GGCGCCTTCC TTTGTACACG TCGTACCACA 
ACACAACAGA AAAAGAAAAG GGCCGCCTGC AAGTCCGCCA ATGGATCCCA CAAGTATAAG 
GGCAACTCCA AAGATCTCAA ACCACCAGAC CTCTGGATCC ACCATGAACG TTTGGAGCTA 
AAGCCCATAG ACAAATCACC CGAACCCAAT CCAGTCATGA CCGACACACC AATCCCCAGG 
ACCTCCCAAG ACATCACGCC GGTGGACGGC TCCATGGATC CTATGCTCCA GAGACGCAAC 
TCTTACCGTG GCCATGAATC AGAGGACAGC ATGTCTACAC TGGCAGGCCG TCGGGGGATG 
AGACCTAAAA TGATGATGCC CTTTGATGCA CAACCCCCTC AGCCTGTGAT TAGTGCTCAC 
CCCATCCATT CCCTCGATAA CCATCACCAT CATTATCACT TGGGCAGCCT GGCCTCGCCG 
ACACGCAGCT ATCTCCACCA CCAGAGCAGC ACGCGCTCCA TCGCCACCCC CATCTCTCAT 
CTAGACAGGG CAGAGTCCAC AGAGTCAGTA AGGAACACCC CTAATGCGGA GCTGCTCCCT 
CCATCTGGGC AGCCATCTTG CACCACTGAC CTTTCAGAGA CGGACATTAG CTTTCACAGC 
TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT ACACCCACAG CACACGTTCG ACCTACACAC 
CCACTTAAGA GTTTTGCAGT GCCTGCCATC CCGGCCCATG CAATGTATGA CTCGGCAGCT 
TTGCCCAGCA CTCCACTGCT GTCTCAGACA GGGCCTCACT CAGTTAAAAC AGCCTCTATT 
GGTACACTGG GAAGGACGAG AACTCCTATG CCTGTCATAG TTCCAAATGC GCCGGATGTC 
TCAGAAGGCT GCAGACTTCA TGAAGATGCA GGAAGTAATT ACGAGACGGA CGAGTTATCG 
GAGGAAATGG CCCATCTCGA GGGCTTAATG AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303466.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301741.1 Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.

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CloneID	ODa39592
Clone ID Related Accession (Same CDS sequence)	XM_009303466.2 , XM_009303466.3
Accession Version	XM_009303466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTCCAGGC 
ATATCTGTGA AACAAGGAAG ACCTTCTGCA CCAGATAACG GCTTTGGATC ACCTGGGAAA 
CCAGACATGT CTGGCACTGG TGATAACCAC ATCCTGGTTA TTGTCATCAT TGTGTCCGTG 
GGTGCTTTTA CCATCATCGT GGTGGTGGTG GGCGCCTTCC TTTGTACACG TCGTACCACA 
ACACAACAGA AAAAGAAAAG GGCCGCCTGC AAGTCCGCCA ATGGATCCCA CAAGTATAAG 
GGCAACTCCA AAGATCTCAA ACCACCAGAC CTCTGGATCC ACCATGAACG TTTGGAGCTA 
AAGCCCATAG ACAAATCACC CGAACCCAAT CCAGTCATGA CCGACACACC AATCCCCAGG 
ACCTCCCAAG ACATCACGCC GGTGGACGGC TCCATGGATC CTATGCTCCA GAGACGCAAC 
TCTTACCGTG GCCATGAATC AGAGGACAGC ATGTCTACAC TGGCAGGCCG TCGGGGGATG 
AGACCTAAAA TGATGATGCC CTTTGATGCA CAACCCCCTC AGCCTGTGAT TAGTGCTCAC 
CCCATCCATT CCCTCGATAA CCATCACCAT CATTATCACT TGGGCAGCCT GGCCTCGCCG 
ACACGCAGCT ATCTCCACCA CCAGAGCAGC ACGCGCTCCA TCGCCACCCC CATCTCTCAT 
CTAGACAGGG CAGAGTCCAC AGAGTCAGTA AGGAACACCC CTAATGCGGA GCTGCTCCCT 
CCATCTGGGC AGCCATCTTG CACCACTGAC CTTTCAGAGA CGGACATTAG CTTTCACAGC 
TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT ACACCCACAG CACACGTTCG ACCTACACAC 
CCACTTAAGA GTTTTGCAGT GCCTGCCATC CCGGCCCATG CAATGTATGA CTCGGCAGCT 
TTGCCCAGCA CTCCACTGCT GTCTCAGACA GGGCCTCACT CAGTTAAAAC AGCCTCTATT 
GGTACACTGG GAAGGACGAG AACTCCTATG CCTGTCATAG TTCCAAATGC GCCGGATGTC 
TCAGAAGGCT GCAGACTTCA TGAAGATGCA GGAAGTAATT ACGAGACGGA CGAGTTATCG 
GAGGAAATGG CCCATCTCGA GGGCTTAATG AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301741.1
CDS	402..4721
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDAQPPQPVISAHPIHSLDNHHHHYHLGSLASPTRSYLHHQSSTRSIATPISHLDRAESTESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303466.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303466.3

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTCCAGGC 
ATATCTGTGA AACAAGGAAG ACCTTCTGCA CCAGATAACG GCTTTGGATC ACCTGGGAAA 
CCAGACATGT CTGGCACTGG TGATAACCAC ATCCTGGTTA TTGTCATCAT TGTGTCCGTG 
GGTGCTTTTA CCATCATCGT GGTGGTGGTG GGCGCCTTCC TTTGTACACG TCGTACCACA 
ACACAACAGA AAAAGAAAAG GGCCGCCTGC AAGTCCGCCA ATGGATCCCA CAAGTATAAG 
GGCAACTCCA AAGATCTCAA ACCACCAGAC CTCTGGATCC ACCATGAACG TTTGGAGCTA 
AAGCCCATAG ACAAATCACC CGAACCCAAT CCAGTCATGA CCGACACACC AATCCCCAGG 
ACCTCCCAAG ACATCACGCC GGTGGACGGC TCCATGGATC CTATGCTCCA GAGACGCAAC 
TCTTACCGTG GCCATGAATC AGAGGACAGC ATGTCTACAC TGGCAGGCCG TCGGGGGATG 
AGACCTAAAA TGATGATGCC CTTTGATGCA CAACCCCCTC AGCCTGTGAT TAGTGCTCAC 
CCCATCCATT CCCTCGATAA CCATCACCAT CATTATCACT TGGGCAGCCT GGCCTCGCCG 
ACACGCAGCT ATCTCCACCA CCAGAGCAGC ACGCGCTCCA TCGCCACCCC CATCTCTCAT 
CTAGACAGGG CAGAGTCCAC AGAGTCAGTA AGGAACACCC CTAATGCGGA GCTGCTCCCT 
CCATCTGGGC AGCCATCTTG CACCACTGAC CTTTCAGAGA CGGACATTAG CTTTCACAGC 
TCAGCCGCTG AGGAGGAGCC AGCGTTCGCT ACACCCACAG CACACGTTCG ACCTACACAC 
CCACTTAAGA GTTTTGCAGT GCCTGCCATC CCGGCCCATG CAATGTATGA CTCGGCAGCT 
TTGCCCAGCA CTCCACTGCT GTCTCAGACA GGGCCTCACT CAGTTAAAAC AGCCTCTATT 
GGTACACTGG GAAGGACGAG AACTCCTATG CCTGTCATAG TTCCAAATGC GCCGGATGTC 
TCAGAAGGCT GCAGACTTCA TGAAGATGCA GGAAGTAATT ACGAGACGGA CGAGTTATCG 
GAGGAAATGG CCCATCTCGA GGGCTTAATG AAGGACCTAA ACGCCATCAC AACAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303468.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301743.1 Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.

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CloneID	ODa39594
Clone ID Related Accession (Same CDS sequence)	XM_009303468.3 , XM_009303468.2
Accession Version	XM_009303468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303468.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGAGTCAGT AAGGAACACC 
CCTAATGCGG AGCTGCTCCC TCCATCTGGG CAGCCATCTT GCACCACTGA CCTTTCAGAG 
ACGGACATTA GCTTTCACAG CTCAGCCGCT GAGGAGGAGC CAGCGTTCGC TACACCCACA 
GCACACGTTC GACCTACACA CCCACTTAAG AGTTTTGCAG TGCCTGCCAT CCCGGCCCAT 
GCAATGTATG ACTCGGCAGC TTTGCCCAGC ACTCCACTGC TGTCTCAGAC AGGGCCTCAC 
TCAGTTAAAA CAGCCTCTAT TGGTACACTG GGAAGGACGA GAACTCCTAT GCCTGTCATA 
GTTCCAAATG CGCCGGATGT CTCAGAAGGC TGCAGACTTC ATGAAGATGC AGGAAGTAAT 
TACGAGACGG ACGAGTTATC GGAGGAAATG GCCCATCTCG AGGGCTTAAT GAAGGACCTA 
AACGCCATCA CAACAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301743.1
CDS	403..4503
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303468.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303468.3

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGAGTCAGT AAGGAACACC 
CCTAATGCGG AGCTGCTCCC TCCATCTGGG CAGCCATCTT GCACCACTGA CCTTTCAGAG 
ACGGACATTA GCTTTCACAG CTCAGCCGCT GAGGAGGAGC CAGCGTTCGC TACACCCACA 
GCACACGTTC GACCTACACA CCCACTTAAG AGTTTTGCAG TGCCTGCCAT CCCGGCCCAT 
GCAATGTATG ACTCGGCAGC TTTGCCCAGC ACTCCACTGC TGTCTCAGAC AGGGCCTCAC 
TCAGTTAAAA CAGCCTCTAT TGGTACACTG GGAAGGACGA GAACTCCTAT GCCTGTCATA 
GTTCCAAATG CGCCGGATGT CTCAGAAGGC TGCAGACTTC ATGAAGATGC AGGAAGTAAT 
TACGAGACGG ACGAGTTATC GGAGGAAATG GCCCATCTCG AGGGCTTAAT GAAGGACCTA 
AACGCCATCA CAACAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1a ( XM_009303468.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301743.1 Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.

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CloneID	ODa39594
Clone ID Related Accession (Same CDS sequence)	XM_009303468.3 , XM_009303468.2
Accession Version	XM_009303468.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neogenin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGAGTCAGT AAGGAACACC 
CCTAATGCGG AGCTGCTCCC TCCATCTGGG CAGCCATCTT GCACCACTGA CCTTTCAGAG 
ACGGACATTA GCTTTCACAG CTCAGCCGCT GAGGAGGAGC CAGCGTTCGC TACACCCACA 
GCACACGTTC GACCTACACA CCCACTTAAG AGTTTTGCAG TGCCTGCCAT CCCGGCCCAT 
GCAATGTATG ACTCGGCAGC TTTGCCCAGC ACTCCACTGC TGTCTCAGAC AGGGCCTCAC 
TCAGTTAAAA CAGCCTCTAT TGGTACACTG GGAAGGACGA GAACTCCTAT GCCTGTCATA 
GTTCCAAATG CGCCGGATGT CTCAGAAGGC TGCAGACTTC ATGAAGATGC AGGAAGTAAT 
TACGAGACGG ACGAGTTATC GGAGGAAATG GCCCATCTCG AGGGCTTAAT GAAGGACCTA 
AACGCCATCA CAACAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301743.1
CDS	402..4502
Translation	MAERGARLLLSLFCLTITVSHAEKGSAVRNFSPFWFSTEPSDTLAVRGAPVLLNCSVHSESPAKIEWKKDGSFLSLASDERRQVLADGSLLISSVVHSKHNKPDEGVYQCVATIDNLGTIISRTARLNVAGIARFLSQPEMASVRVGDSQVLSCEVNPDLVSFIRWEQNKEAVELDHRVFSLPSGALVISNATETDAGLYRCVIDNAGPTKTSEEAQLHILTETGEERTLEFLQEPQHVSKLVGESVLLPCVVTGYPTPEITWMYKDQLIEDSSGRFEILGGGSLRIFNLTEEDAGVYNCLAENTNGSIEAQAELTLKDSPQFLKKPVNVFAHEATDVIFECEVSGSPSPTIKWVKNGDAVIPSDYFKIIKEQNLQVLGLVKSDEGFYQCLAENDAGNVQSSAQLVILDQDVTLPLPRPTSLTRATTDRLMPGSRGGAGPTPSAPRDVVASLVSTRFLKLTWRLPAEPHGDDVTYSVYYSLEGTNRERIVNTSRPGEMQVTIQNLMPDTKYAFRVVAHNKNGPGESSVPLKVETQPEVQVPGPAPNLHAVVMSPSTVSLSWDVPLIGNGEIQNYKIYYMEKGMDSEQDLDVNTLSYTMTGLKKFTEYSFRLVAYNKHGPGVSTEDISVRTYSDVPSSPPQNMTVEVLNSKSLMIRWQPPPADAQNGEITGYKIRYRKGTRKSEVAEITSGSQLYQLIDGLQRGTEYMLRVSAMTVNGTGPATDWTTAETFESDLDESRVPDVPSSLHVRSLVNSIVVSWTPPENQDIVVRGYSISYGIGSPHAQTIKVDYKQRYYTIENLNPNSHYVITLKAFNNVGEGIPVYESAITRPQSDPIDPDVDLYELFHAPYTPVPDPIPMLPPVGVQASVLNQDTIKVTWADNSLPKNQKITDARYYTVRWKTNIPANTKFKVANTTTLFHTVTGLKPNTLYEFSVMVTKGRRTSTWSMTAHGTTFESIPSSPPKDVTVVSKEGRPKTIIVNWQPPSEANGKITGYIIYYSTDVKAEVHDWVIEPVVGNRLTHQIQELTLDTTYYFKIQARNSKGMGPMSEAVQFRTPKAESSDKMANDQAPGISVKQGRPSAPDNGFGSPGKPDMSGTGDNHILVIVIIVSVGAFTIIVVVVGAFLCTRRTTTQQKKKRAACKSANGSHKYKGNSKDLKPPDLWIHHERLELKPIDKSPEPNPVMTDTPIPRTSQDITPVDGSMDPMLQRRNSYRESVRNTPNAELLPPSGQPSCTTDLSETDISFHSSAAEEEPAFATPTAHVRPTHPLKSFAVPAIPAHAMYDSAALPSTPLLSQTGPHSVKTASIGTLGRTRTPMPVIVPNAPDVSEGCRLHEDAGSNYETDELSEEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_009303468.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neogenin 1a (neo1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303468.2

ORF Insert Sequence:

ATGGCGGAGC GGGGAGCCCG GCTGCTCCTC TCTCTTTTCT GCCTCACGAT CACAGTTTCA 
CATGCTGAAA AAGGATCAGC AGTGAGGAAC TTCAGCCCAT TCTGGTTCTC CACGGAGCCG 
TCGGATACAC TAGCGGTGCG AGGAGCGCCA GTTCTCCTCA ACTGCTCAGT CCACAGCGAG 
TCTCCTGCCA AGATCGAGTG GAAAAAAGAT GGCTCTTTCC TCAGCCTGGC TTCAGACGAG 
CGCAGACAGG TCCTGGCCGA TGGCTCTCTG CTCATCAGCT CAGTGGTGCA CTCCAAACAC 
AATAAACCCG ATGAGGGGGT TTACCAGTGC GTGGCTACTA TAGACAACCT GGGCACCATC 
ATTAGCCGTA CAGCTAGACT CAACGTAGCA GGTATAGCGC GGTTCCTCAG TCAGCCTGAG 
ATGGCGTCGG TGCGGGTGGG CGACAGTCAA GTGTTGAGCT GTGAAGTCAA CCCAGACCTG 
GTGTCCTTCA TCCGCTGGGA ACAGAATAAA GAGGCGGTGG AGCTGGACCA CAGAGTCTTC 
AGTCTGCCCA GTGGAGCTCT GGTTATCAGT AACGCCACAG AGACAGACGC GGGTCTGTAC 
CGCTGTGTCA TCGACAACGC TGGACCCACC AAGACCAGTG AAGAAGCACA GTTGCACATA 
CTGACAGAAA CGGGTGAGGA AAGGACGCTG GAGTTTCTCC AGGAGCCTCA ACATGTGTCT 
AAACTGGTCG GCGAAAGTGT CCTGCTCCCC TGTGTGGTGA CGGGATATCC CACTCCTGAA 
ATCACATGGA TGTACAAAGA CCAGCTCATT GAGGACAGCA GTGGCCGCTT TGAGATACTC 
GGCGGGGGGA GTCTGCGGAT CTTCAATCTC ACAGAGGAGG ATGCCGGGGT GTACAACTGT 
CTCGCAGAAA ACACCAATGG GTCCATTGAG GCCCAGGCTG AGCTCACGCT GAAGGATTCT 
CCACAGTTCC TGAAGAAACC TGTGAATGTG TTTGCTCATG AAGCCACAGA CGTCATCTTC 
GAGTGCGAGG TCTCAGGATC ACCTTCTCCC ACCATCAAAT GGGTGAAAAA TGGAGACGCC 
GTCATCCCAA GCGATTATTT CAAAATCATT AAGGAGCAGA ACCTGCAGGT GTTGGGACTG 
GTCAAGTCAG ATGAAGGTTT TTATCAGTGT TTAGCTGAGA ATGACGCTGG CAACGTGCAG 
TCAAGTGCTC AGCTCGTCAT ATTGGATCAA GATGTAACCC TCCCCTTACC CCGTCCCACC 
TCACTGACCC GTGCTACTAC TGACCGTCTA ATGCCAGGCT CCAGAGGCGG CGCTGGCCCC 
ACGCCCTCCG CCCCCCGTGA TGTGGTAGCG TCCCTGGTGT CCACCCGCTT CCTCAAGCTG 
ACCTGGCGCC TTCCTGCTGA ACCACACGGA GACGATGTCA CCTACTCAGT CTACTACAGC 
CTGGAGGGCA CTAACAGGGA GAGGATCGTA AACACCAGTC GTCCAGGAGA AATGCAAGTC 
ACCATTCAGA ACCTCATGCC AGACACCAAG TATGCCTTCC GTGTGGTGGC GCACAACAAA 
AACGGCCCTG GAGAGAGTTC AGTGCCTCTA AAGGTCGAAA CTCAGCCCGA AGTCCAGGTA 
CCTGGTCCGG CTCCAAACCT GCATGCAGTG GTCATGTCCC CCAGCACTGT CAGTCTCAGC 
TGGGATGTGC CTCTTATAGG CAATGGTGAG ATCCAGAACT ACAAGATCTA CTACATGGAG 
AAGGGCATGG ACAGTGAACA GGATCTGGAT GTAAACACAT TGTCCTACAC CATGACTGGC 
CTGAAGAAGT TCACGGAGTA CAGTTTCAGA CTGGTGGCCT ATAACAAACA TGGACCGGGA 
GTCTCCACTG AGGACATATC AGTGCGCACA TACTCTGATG TCCCCAGCTC TCCTCCGCAG 
AACATGACGG TGGAAGTGCT GAACTCCAAG AGCCTCATGA TCAGATGGCA GCCTCCACCA 
GCCGATGCCC AGAACGGAGA GATCACAGGC TACAAGATCC GCTACAGGAA GGGTACGAGG 
AAGAGCGAGG TGGCGGAGAT CACTTCAGGA TCACAGCTTT ATCAGCTCAT AGATGGTTTG 
CAGCGAGGCA CTGAATATAT GTTGCGAGTA TCTGCAATGA CGGTCAACGG CACAGGTCCT 
GCCACAGACT GGACCACTGC AGAGACATTC GAAAGCGATC TGGACGAGTC AAGAGTGCCG 
GACGTCCCGA GCTCTCTGCA TGTTCGTTCT CTGGTCAACA GTATTGTGGT GAGCTGGACG 
CCTCCAGAGA ACCAGGACAT CGTGGTGAGG GGTTACAGTA TCAGCTATGG GATTGGCAGT 
CCTCACGCAC AGACCATCAA AGTGGACTAC AAGCAGAGAT ACTACACCAT TGAGAACCTG 
AATCCCAACT CGCACTACGT GATCACGCTG AAGGCCTTCA ACAACGTGGG TGAGGGAATC 
CCTGTGTACG AGAGCGCCAT CACACGACCA CAGTCAGACC CCATAGACCC TGACGTTGAT 
TTGTACGAAC TTTTCCATGC TCCATACACT CCAGTACCAG ACCCTATACC CATGCTGCCT 
CCTGTGGGCG TTCAGGCATC TGTCCTGAAC CAAGACACCA TCAAAGTCAC CTGGGCGGAC 
AACTCGCTGC CTAAGAACCA GAAGATCACA GATGCGCGCT ACTATACAGT GCGCTGGAAG 
ACCAACATAC CCGCAAACAC AAAGTTTAAG GTGGCTAATA CCACGACGCT ATTTCACACG 
GTGACGGGTC TTAAACCCAA TACCTTGTAT GAGTTTTCGG TTATGGTGAC CAAGGGACGG 
AGGACCAGTA CCTGGAGCAT GACTGCACAC GGAACAACTT TTGAGTCAAT CCCAAGTTCT 
CCACCAAAAG ATGTGACTGT AGTGAGCAAA GAGGGACGAC CAAAAACCAT CATCGTCAAC 
TGGCAGCCGC CATCAGAAGC CAATGGCAAA ATTACAGGTT ACATCATTTA TTACAGCACT 
GATGTGAAGG CAGAGGTTCA TGACTGGGTC ATTGAGCCAG TCGTGGGAAA CAGGCTAACC 
CATCAAATTC AGGAGCTGAC CCTTGATACC ACCTACTACT TTAAGATCCA GGCTCGGAAC 
TCCAAGGGCA TGGGTCCCAT GTCGGAAGCG GTCCAGTTCC GCACTCCTAA AGCTGAATCC 
TCTGACAAAA TGGCTAATGA CCAAGCTCCA GGCATATCTG TGAAACAAGG AAGACCTTCT 
GCACCAGATA ACGGCTTTGG ATCACCTGGG AAACCAGACA TGTCTGGCAC TGGTGATAAC 
CACATCCTGG TTATTGTCAT CATTGTGTCC GTGGGTGCTT TTACCATCAT CGTGGTGGTG 
GTGGGCGCCT TCCTTTGTAC ACGTCGTACC ACAACACAAC AGAAAAAGAA AAGGGCCGCC 
TGCAAGTCCG CCAATGGATC CCACAAGTAT AAGGGCAACT CCAAAGATCT CAAACCACCA 
GACCTCTGGA TCCACCATGA ACGTTTGGAG CTAAAGCCCA TAGACAAATC ACCCGAACCC 
AATCCAGTCA TGACCGACAC ACCAATCCCC AGGACCTCCC AAGACATCAC GCCGGTGGAC 
GGCTCCATGG ATCCTATGCT CCAGAGACGC AACTCTTACC GTGAGTCAGT AAGGAACACC 
CCTAATGCGG AGCTGCTCCC TCCATCTGGG CAGCCATCTT GCACCACTGA CCTTTCAGAG 
ACGGACATTA GCTTTCACAG CTCAGCCGCT GAGGAGGAGC CAGCGTTCGC TACACCCACA 
GCACACGTTC GACCTACACA CCCACTTAAG AGTTTTGCAG TGCCTGCCAT CCCGGCCCAT 
GCAATGTATG ACTCGGCAGC TTTGCCCAGC ACTCCACTGC TGTCTCAGAC AGGGCCTCAC 
TCAGTTAAAA CAGCCTCTAT TGGTACACTG GGAAGGACGA GAACTCCTAT GCCTGTCATA 
GTTCCAAATG CGCCGGATGT CTCAGAAGGC TGCAGACTTC ATGAAGATGC AGGAAGTAAT 
TACGAGACGG ACGAGTTATC GGAGGAAATG GCCCATCTCG AGGGCTTAAT GAAGGACCTA 
AACGCCATCA CAACAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	neo1a
Entrez Gene ID	266983
Full Name	neogenin 1a
Synonyms	cb333,neo1,zgc:109795,zneo1
General protein information	Preferred Names neogenin 1a Names neogenin neogenin 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Danio rerio (zebrafish)	neo1	NP_775325.1
Canis lupus familiaris (dog)	NEO1	XP_005638634.1
Bos taurus (cattle)	NEO1	XP_005211488.1
Mus musculus (house mouse)	Neo1	NP_032710.2
Drosophila melanogaster (fruit fly)	fra	NP_725185.1
Homo sapiens (human)	NEO1	NP_002490.2
Pan troglodytes (chimpanzee)	NEO1	XP_510660.3
	NEO1	XP_002804901.1
Rattus norvegicus (Norway rat)	Neo1	XP_006243248.1
Gallus gallus (chicken)	NEO1	XP_004943713.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006083	XP_316139.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

neo1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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