MPP5A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MPP5A
Entrez Gene ID	252845
Full Name	membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5)
Synonyms	mpp5,nok
General protein information	Preferred Names membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) Names MAGUK p55 subfamily member 5-A MAGUK family factor membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5a) nagie oko protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293174.3	XP_009291449.1	MAGUK p55 subfamily member 5-A isoform X1
NM_194363.1	NP_919344.1	MAGUK p55 subfamily member 5-A
XM_009293174.2	XP_009291449.1	MAGUK p55 subfamily member 5-A isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	mpp5	XP_002937246.1
Bos taurus (cattle)	MPP5	NP_001192880.1
Mus musculus (house mouse)	Mpp5	NP_062525.1
Canis lupus familiaris (dog)	MPP5	XP_005623574.1
Gallus gallus (chicken)	MPP5	NP_001186634.1
Drosophila melanogaster (fruit fly)	sdt	NP_001245576.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002711	XP_312216.5
Homo sapiens (human)	MPP5	NP_071919.2
Pan troglodytes (chimpanzee)	MPP5	XP_003314448.1
	MPP5	XP_001105651.2
Rattus norvegicus (Norway rat)	Mpp5	NP_001101504.1
Danio rerio (zebrafish)	mpp5a	NP_919344.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Discovering miRNA Regulatory Networks in Holt-Oram Syndrome Using a Zebrafish Model.
D'Aurizio R, Russo F, Chiavacci E, Baumgart M, Groth M, D'Onofrio M, Arisi I, Rainaldi G, Pitto L, Pellegrini M
Frontiers in bioengineering and biotechnology460(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.
Zou J, Wen Y, Yang X, Wei X
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(8)770-82(2013 Dec)

Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant.
Ichimura K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Obara T
Developmental dynamics : an official publication of the American Association of Anatomists241(12)1922-32(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study shows that Spatial-temporal expressions of Nagie oko and their interdependence in zebrafish central nervous system during early development.
Title: Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.

The structural disorganization of the pronephric glomerulus in the mpp5a(m520) mutant is likely to result from circulatory failure, rather than the anomaly of Mpp5a protein in the glomerulus.
Title: Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant.

the ratio of the number of cells undergoing neurogenic cell division to the total number of cells undergoing mitosis was decreased in retinas of nagie oko knockout zebrafish.
Title: Mutations in N-cadherin and a Stardust homolog, Nagie oko, affect cell-cycle exit in zebrafish retina.

that Nok is required for retinal pigment epithelium integrity in a tissue-autonomous manner
Title: Intact retinal pigment epithelium maintained by Nok is essential for retinal epithelial polarity and cellular patterning in zebrafish.

deletion constructs of Nok in functional rescue experiments to define domains essential for cell polarity, maintenance of epithelial integrity and cardiac morphogenesis.Nok does not interact with Crumbs proteins upon deletion of the PDZ domain
Title: Divergent polarization mechanisms during vertebrate epithelial development mediated by the Crumbs complex protein Nagie oko.

The following MPP5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPP5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03721	NM_194363.1 Latest version!	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39571	XM_009293174.3 Latest version!	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa39571	XM_009293174.2	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MPP5A ( NM_194363.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_919344.1 Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), mRNA.

Price & Availability↑

CloneID	ODa03721
Clone ID Related Accession (Same CDS sequence)	NM_194363.1
Accession Version	NM_194363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	MAGUK p55 subfamily member 5-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF510111.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF510111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA GTTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGTGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGTCTGCTGC ATGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATTCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ACGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT TGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAAACA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCAAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGAAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919344.1
CDS	339..2450
Translation	MQKAPLLTLIPHTHLHHTPPHMRAAMMTTSHMNGYVTESDGGGGTDIVEEGVQRHREMAVDCPSELGARTLPVRRSAQLEKIRQQQEDRRRREEEGRSRQELDLNSSMRLKKLSQNPKVGIDNPTFEQMEGPGGSAGGLQSLIAPPALLELEDLLMSLKQVQHSLNDSQSQEDVELVLQLVQKPDFQKAFSIHNSVAHYMNRPSPPYPLTDHAQTLAQEVEVMVQNSSHKEGLELSSLLSTSHMQALMMAHDSVAEQEMQLEPLVPSVNASETLTQWGGETVKIVRIEKAKDIPLGATVRNDMDSVVISRIVKGGAAERSGLLHEGDEILEINGVEIRGKDVNEVFDILADMHGVLSFVLIPSAQIKSPPIKETVVHVKAHFDYDPSDDPYVPCRELGLCFQKGDILHIISQDDPNWWQAYRDGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKTGKLWCAKKTKKKRKKMQYNANKNDDFDNEEILTYEEMALYHQPANRKRPIALIGPPNCGQNELRQRLLSTEPDRFAGPVPHTTRSRRDAEANGRDYHFVSRQAFEMDSAAGKFIESGEFEKNFYGTSTDSVRQVINTGKICLLCVHTQSLKVLRSSDLKPYIIFIAPPSQERLRALLAKDNKNPKPEELRDIIEKAREMEQNYGHLFDAAIVNTDLDKSYQELLRLINKLDTEPQWVPSSWLR

Target ORF information:

RefSeq Version	NM_194363.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194363.1

ORF Insert Sequence:

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA GTTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGTGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGTCTGCTGC ATGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATTCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ACGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT TGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAAACA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCAAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGAAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MPP5A ( XM_009293174.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291449.1 Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39571
Clone ID Related Accession (Same CDS sequence)	XM_009293174.3 , XM_009293174.2
Accession Version	XM_009293174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MAGUK p55 subfamily member 5-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA ATTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGCGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGCCTGCTAC ACGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATCCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ATGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT CGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAATCA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCGAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGCAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291449.1
CDS	441..2552
Translation	MQKAPLLTLIPHTHLHHTPPHMRAAMMTTSHMNGYVTESDGGGGTDIVEEGVQRHREMAVDCPSELGARTLPVRRSAQLEKIRQQQEDRRRREEEGRSRQELDLNSSMRLKKLSQNPKVGIDNPTFEQMEGPGGSAGGLQSLIAPPALLELEDLLMSLKQVQHNLNDSQSQEDVELVLQLVQKPDFQKAFSIHNSVAHYMNRPSPPYPLTDHAQTLAQEVEVMVQNSSHKEGLELSSLLSTSHMQALMMAHDSVAEQEMQLEPLVPSVNASETLTQWGGETVKIVRIEKAKDIPLGATVRNDMDSVVISRIVKGGAAERSGLLHEGDEILEINGVEIRGKDVNEVFDILADMHGVLSFVLIPSAQIKSPPIKETVVHVKAHFDYDPSDDPYVPCRELGLCFQKGDILHIISQDDPNWWQAYRDGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKTKKKRKKMQYNANKNDDFDNEEILTYEEMALYHQPANRKRPIALIGPPNCGQNELRQRLLSTEPDRFAGPVPHTTRSRRDAEANGRDYHFVSRQAFEMDSAAGKFIESGEFEKNFYGTSTDSVRQVINTGKICLLCVHTQSLKVLRSSDLKPYIIFIAPPSQERLRALLAKDNKNPKPEELRDIIEKAREMEQNYGHLFDAAIVNTDLDKSYQELLRLINKLDTEPQWVPSSWLR

Target ORF information:

RefSeq Version	XM_009293174.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293174.3

ORF Insert Sequence:

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA ATTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGCGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGCCTGCTAC ACGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATCCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ATGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT CGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAATCA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCGAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGCAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MPP5A ( XM_009293174.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291449.1 Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.

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CloneID	ODa39571
Clone ID Related Accession (Same CDS sequence)	XM_009293174.3 , XM_009293174.2
Accession Version	XM_009293174.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	MAGUK p55 subfamily member 5-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA ATTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGCGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGCCTGCTAC ACGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATCCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ATGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT CGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAATCA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCGAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGCAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291449.1
CDS	419..2530
Translation	MQKAPLLTLIPHTHLHHTPPHMRAAMMTTSHMNGYVTESDGGGGTDIVEEGVQRHREMAVDCPSELGARTLPVRRSAQLEKIRQQQEDRRRREEEGRSRQELDLNSSMRLKKLSQNPKVGIDNPTFEQMEGPGGSAGGLQSLIAPPALLELEDLLMSLKQVQHNLNDSQSQEDVELVLQLVQKPDFQKAFSIHNSVAHYMNRPSPPYPLTDHAQTLAQEVEVMVQNSSHKEGLELSSLLSTSHMQALMMAHDSVAEQEMQLEPLVPSVNASETLTQWGGETVKIVRIEKAKDIPLGATVRNDMDSVVISRIVKGGAAERSGLLHEGDEILEINGVEIRGKDVNEVFDILADMHGVLSFVLIPSAQIKSPPIKETVVHVKAHFDYDPSDDPYVPCRELGLCFQKGDILHIISQDDPNWWQAYRDGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKTKKKRKKMQYNANKNDDFDNEEILTYEEMALYHQPANRKRPIALIGPPNCGQNELRQRLLSTEPDRFAGPVPHTTRSRRDAEANGRDYHFVSRQAFEMDSAAGKFIESGEFEKNFYGTSTDSVRQVINTGKICLLCVHTQSLKVLRSSDLKPYIIFIAPPSQERLRALLAKDNKNPKPEELRDIIEKAREMEQNYGHLFDAAIVNTDLDKSYQELLRLINKLDTEPQWVPSSWLR

Target ORF information:

RefSeq Version	XM_009293174.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) (mpp5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293174.2

ORF Insert Sequence:

ATGCAGAAAG CACCCCTCCT AACCCTCATA CCTCACACAC ACTTGCACCA CACACCACCA 
CACATGCGCG CAGCCATGAT GACCACCTCC CACATGAACG GATATGTGAC CGAGTCAGAT 
GGCGGGGGAG GTACTGACAT CGTGGAAGAG GGGGTGCAGA GGCACAGGGA GATGGCGGTG 
GACTGCCCAA GTGAGCTGGG TGCCCGCACC CTGCCCGTCA GACGCAGCGC TCAGCTGGAA 
AAGATCCGCC AGCAGCAGGA GGACCGCAGA AGACGAGAGG AGGAGGGCCG CAGCCGGCAG 
GAGCTTGACC TCAACTCGTC CATGCGCCTC AAGAAACTCT CCCAGAACCC AAAAGTGGGC 
ATCGATAACC CCACCTTTGA GCAAATGGAA GGTCCTGGTG GCTCCGCAGG TGGTCTTCAG 
AGCCTCATCG CACCACCAGC TTTACTAGAG CTGGAGGACC TGTTGATGTC CCTTAAGCAG 
GTGCAGCATA ATTTGAATGA CTCTCAAAGT CAAGAGGACG TGGAGCTGGT TCTACAGCTG 
GTCCAGAAGC CTGACTTCCA GAAAGCCTTC AGCATTCACA ACTCTGTGGC CCACTATATG 
AACCGGCCCA GCCCCCCCTA CCCCCTGACG GATCACGCAC AGACCCTGGC TCAAGAGGTG 
GAGGTCATGG TGCAAAACAG CTCACACAAG GAAGGACTTG AGCTAAGCAG CCTGCTCTCA 
ACGTCCCACA TGCAGGCGCT CATGATGGCA CATGACAGCG TGGCTGAACA AGAGATGCAG 
CTGGAGCCTC TTGTTCCTTC TGTCAATGCA AGCGAGACTC TCACCCAGTG GGGAGGAGAG 
ACTGTCAAAA TTGTACGGAT TGAAAAAGCC AAGGACATCC CATTGGGAGC GACTGTTAGA 
AATGATATGG ACAGTGTGGT CATCAGCCGG ATTGTTAAAG GGGGGGCGGC AGAACGCAGC 
GGCCTGCTAC ACGAAGGAGA CGAGATCCTG GAGATCAACG GTGTGGAAAT CCGTGGCAAA 
GATGTCAACG AAGTCTTCGA CATCCTGGCG GACATGCATG GTGTACTGTC TTTTGTTCTC 
ATCCCCAGCG CACAGATCAA ATCTCCTCCT ATTAAAGAGA CTGTGGTACA TGTGAAGGCA 
CATTTTGACT ATGACCCCTC AGATGACCCA TATGTGCCAT GCCGAGAGCT CGGCCTGTGC 
TTTCAGAAGG GGGATATACT CCACATAATC AGCCAAGACG ACCCCAACTG GTGGCAGGCC 
TACAGAGATG GGGATGAAGA CAACCAGCCT CTTGCTGGCC TCGTCCCTGG CAAAAGTTTT 
CAGCAGCAGA GGGAGGCAAT GAAGCAAACC ATAGAAGAGG ATAAAGAGCC TGAAAAATCA 
GGAAAGCTTT GGTGTGCTAA AAAGACCAAG AAGAAGCGGA AGAAGATGCA ATACAATGCT 
AACAAAAATG ATGACTTTGA TAATGAGGAG ATTCTCACAT ATGAAGAAAT GGCACTTTAC 
CACCAACCAG CCAATCGTAA GCGGCCCATC GCACTAATCG GCCCACCGAA CTGTGGGCAA 
AATGAGCTCA GACAAAGACT CCTCTCCACT GAGCCGGACA GGTTTGCTGG ACCTGTTCCT 
CACACCACAC GGAGTCGACG TGACGCTGAG GCGAACGGCC GTGACTACCA CTTTGTGTCC 
CGCCAGGCCT TCGAGATGGA CTCTGCAGCA GGGAAATTCA TAGAGTCCGG AGAGTTTGAA 
AAGAACTTTT ACGGCACCAG CACAGACTCT GTCCGACAAG TCATCAACAC AGGCAAAATC 
TGCCTCCTGT GCGTACACAC CCAGTCTTTG AAGGTGTTGC GCAGCTCGGA CCTCAAGCCC 
TACATCATCT TCATCGCTCC TCCATCCCAA GAGCGATTGA GAGCCCTCTT AGCTAAAGAC 
AACAAAAACC CAAAGCCCGA GGAGCTGCGA GACATTATCG AGAAGGCCCG AGAGATGGAG 
CAGAACTACG GCCACCTGTT TGATGCTGCC ATTGTGAACA CCGACCTGGA CAAATCCTAC 
CAGGAGCTGC TGCGTCTCAT CAATAAACTA GACACTGAAC CCCAGTGGGT CCCTTCATCC 
TGGCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Discovering miRNA Regulatory Networks in Holt-Oram Syndrome Using a Zebrafish Model.
Frontiers in bioengineering and biotechnology460(2016)
D'Aurizio R,Russo F,Chiavacci E,Baumgart M,Groth M,D'Onofrio M,Arisi I,Rainaldi G,Pitto L,Pellegrini M

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(8)770-82(2013 Dec)
Zou J,Wen Y,Yang X,Wei X

Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant.
Developmental dynamics : an official publication of the American Association of Anatomists241(12)1922-32(2012 Dec)
Ichimura K,Fukuyo Y,Nakamura T,Powell R,Sakai T,Obara T

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MPP5A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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