phc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phc2a
Entrez Gene ID	171465
Full Name	polyhomeotic homolog 2a (Drosophila)
Synonyms	cb218,edr2,ph2,ph2[a],ph2[b],ph2alpha,ph2beta,phc2
General protein information	Preferred Names polyhomeotic homolog 2a (Drosophila) Names polyhomeotic-like protein 2 early development regulator 2 (homolog of polyhomeotic 2)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296803.2	XP_009295078.1	polyhomeotic-like protein 2 isoform X1
XM_009296802.2	XP_009295077.1	polyhomeotic-like protein 2 isoform X1
XM_005162144.3	XP_005162201.1	polyhomeotic-like protein 2 isoform X1
NM_001136488.2	NP_001129960.1	polyhomeotic-like protein 2 isoform b
NM_152958.2	NP_694490.2	polyhomeotic-like protein 2 isoform a
XM_005162144.4	XP_005162201.1	polyhomeotic-like protein 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PHC2	XP_005617661.1
Rattus norvegicus (Norway rat)	Phc2	NP_001013187.2
Danio rerio (zebrafish)	phc2b	NP_957496.3
Homo sapiens (human)	PHC2	NP_932157.1
Pan troglodytes (chimpanzee)	PHC2	XP_001165724.1
	PHC2	XP_002802343.1
Bos taurus (cattle)	PHC2	NP_001179837.1
Mus musculus (house mouse)	Phc2	NP_061244.1
Danio rerio (zebrafish)	phc2a	NP_694490.1
Gallus gallus (chicken)	PHC2	XP_003642590.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Phenotypic chemical screening using a zebrafish neural crest EMT reporter identifies retinoic acid as an inhibitor of epithelial morphogenesis.
Jimenez L, Wang J, Morrison MA, Whatcott C, Soh KK, Warner S, Bearss D, Jette CA, Stewart RA
Disease models & mechanisms9(4)389-400(2016 Apr)

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Aanes H, Winata CL, Lin CH, Chen JP, Srinivasan KG, Lee SG, Lim AY, Hajan HS, Collas P, Bourque G, Gong Z, Korzh V, Alestr?m P, Mathavan S
Genome research21(8)1328-38(2011 Aug)

The zebrafish genes encoding the Polycomb repressive complex (PRC) 1.
Le Faou P, V?lkel P, Angrand PO
Gene475(1)10-21(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, the data argue that ph2alpha is required for epiboly and tailbud formation, acting downstream of the FGF signaling pathway
Title: Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling.

The following phc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02889	NM_001136488.2 Latest version!	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa27687	NM_152958.2 Latest version!	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27687	XM_009296803.2 Latest version!	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27687	XM_009296802.2 Latest version!	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27687	XM_005162144.4 Latest version!	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa27687	XM_005162144.3	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phc2a ( NM_001136488.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001129960.1 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa02889
Clone ID Related Accession (Same CDS sequence)	NM_001136488.2
Accession Version	NM_001136488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Danio rerio(zebrafish)
Product	polyhomeotic-like protein 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005123.16. On May 12, 2015 this sequence version replaced NM_001136488.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY398396.1, BC076431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACCTCGG GCAGCGGTAA TAATGCGCCC ACGGTGACCG GCAGCGCTCC GCAAAATGGC 
GAGAGTAAAC CGCCGCCACA GGCTGTAGTG AAGCCCCAGA TCCTCACTCA TGTCATCGAG 
GGATTCGTGA TTCAGGAGGG AGCGGAGCCT TTTCCCGTTG AACGTCCGTC CTTGCTGATC 
GAGAATTTAA AACAGAAGCA GCATCATGCT TATTCGGATC TACAGAAGCA CAACGCAGAC 
TCTGAGATGG AGGATCTGTC TCTGCAAGAG CTGAATAATC AACCCGAGCC AGTGCGGACA 
TGTGAATTCT GTGGGAATGT AGACTTCGCA TTCAACTTCA AGAGATCCAA GCGGTTTTGC 
TCTACAGTGT GTGCGAAGAG ATATAATGTT GGATGCACAA AGCGAATGGG CCTTTTTCCA 
GGGAAATCCA GCCCGGAGGA CACAAAGAAA CCAAAAGCCT CAGATGAAAG TCCTAAAAAC 
TGCAGCACAG AAACCAGGAA ACGGAATCCA AGCATACAAA CGACCACAGG AGCTTCTTTG 
TTGTCCCCAC ATCCATCTCA TCCAAGTCAT GGTGAGTCCA GCCAGTGCTC AGACATGTCC 
AGCTATGAAG AGCCCATCTC ACCACTGTCC AACTCGAGCT TTGGAGCCCC GATTGAGCAC 
GAGGAAAGTT TTGATCACAG CAGAGAGCTT ACTCCTCTAC TCACGCAGCA CTTCCTGGCC 
AGCGACCCCA CAAAGTGGAA CGTCGAGGAT GTTTATGAAT TCATCTGCTC TCTGCCAGGT 
TGCCATGAGA TCGCTGAAGA GTTTCGCTCT CAGGAGATTG ATGGCCAGGC CTTGATGCTC 
CTCAAAGAGG ATCATCTCAT GAGCACAATG AACATCAAAC TGGGCCCTGC ACTGAAGATC 
TTCGCCCGCA TCAGTATGCT CAAGGACTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129960.1
CDS	260..1192
Translation	MTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	NM_001136488.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136488.2

ORF Insert Sequence:

ATGACCTCGG GCAGCGGTAA TAATGCGCCC ACGGTGACCG GCAGCGCTCC GCAAAATGGC 
GAGAGTAAAC CGCCGCCACA GGCTGTAGTG AAGCCCCAGA TCCTCACTCA TGTCATCGAG 
GGATTCGTGA TTCAGGAGGG AGCGGAGCCT TTTCCCGTTG AACGTCCGTC CTTGCTGATC 
GAGAATTTAA AACAGAAGCA GCATCATGCT TATTCGGATC TACAGAAGCA CAACGCAGAC 
TCTGAGATGG AGGATCTGTC TCTGCAAGAG CTGAATAATC AACCCGAGCC AGTGCGGACA 
TGTGAATTCT GTGGGAATGT AGACTTCGCA TTCAACTTCA AGAGATCCAA GCGGTTTTGC 
TCTACAGTGT GTGCGAAGAG ATATAATGTT GGATGCACAA AGCGAATGGG CCTTTTTCCA 
GGGAAATCCA GCCCGGAGGA CACAAAGAAA CCAAAAGCCT CAGATGAAAG TCCTAAAAAC 
TGCAGCACAG AAACCAGGAA ACGGAATCCA AGCATACAAA CGACCACAGG AGCTTCTTTG 
TTGTCCCCAC ATCCATCTCA TCCAAGTCAT GGTGAGTCCA GCCAGTGCTC AGACATGTCC 
AGCTATGAAG AGCCCATCTC ACCACTGTCC AACTCGAGCT TTGGAGCCCC GATTGAGCAC 
GAGGAAAGTT TTGATCACAG CAGAGAGCTT ACTCCTCTAC TCACGCAGCA CTTCCTGGCC 
AGCGACCCCA CAAAGTGGAA CGTCGAGGAT GTTTATGAAT TCATCTGCTC TCTGCCAGGT 
TGCCATGAGA TCGCTGAAGA GTTTCGCTCT CAGGAGATTG ATGGCCAGGC CTTGATGCTC 
CTCAAAGAGG ATCATCTCAT GAGCACAATG AACATCAAAC TGGGCCCTGC ACTGAAGATC 
TTCGCCCGCA TCAGTATGCT CAAGGACTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phc2a ( NM_152958.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694490.2 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa27687
Clone ID Related Accession (Same CDS sequence)	XM_009296803.2 , XM_009296802.2 , XM_005162144.3 , NM_152958.2 , XM_005162144.4
Accession Version	NM_152958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Danio rerio(zebrafish)
Product	polyhomeotic-like protein 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772391.18 and BX005123.16. On May 12, 2015 this sequence version replaced NM_152958.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB064940.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694490.2
CDS	118..2601
Translation	MEKEQEQGSVASSASVTIPSTTSVSTSTSAGTLSNSSSRQQTVPQISVYGGITDRQTVQVIQQALNRQPSTVAAQYLQQMYAAQQQHLMLQTAALQQQHLSLAAVQQASIVAGRQSCSQHGSTSQQTVASQTTINLATSPAAAHLISRAQSGSSVPVCIGQQAVLLGNSSTPTLTASQAQMYLRAQMAQQTNLVQVARSLGRAVPLSSQLIFTPTASVTAIQPEAPAPSINTLPTSGQVQNLALRSQQGALTSTLSQSQLQSLSVKQSVASVTGQSVARLKGLGAEPSPQGTAAKASPAETSSETTAKNDKTSDLTTSVCPSVTSVAGHPFISTAYTQIQTHQLLQQHKQQFVIQQQPQILQRGQAQLLEAAAIHPHTVQAVAIQSALPAQPQQCPIPLLPKVPVTCQQATIFHSTTVSQQALAQNGQAHILTNTKAPPLQLTAVKCQIHPVQIQQGVASQNVPDKDTPVLLEPQLLSIPIQEPTRTELRQSDKESQVKENTQGSVNVKAGVGSPPAMTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	NM_152958.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152958.2

ORF Insert Sequence:

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phc2a ( XM_009296803.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295078.1 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27687
Clone ID Related Accession (Same CDS sequence)	XM_009296803.2 , XM_009296802.2 , XM_005162144.3 , NM_152958.2 , XM_005162144.4
Accession Version	XM_009296803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	polyhomeotic-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295078.1
CDS	325..2808
Translation	MEKEQEQGSVASSASVTIPSTTSVSTSTSAGTLSNSSSRQQTVPQISVYGGITDRQTVQVIQQALNRQPSTVAAQYLQQMYAAQQQHLMLQTAALQQQHLSLAAVQQASIVAGRQSCSQHGSTSQQTVASQTTINLATSPAAAHLISRAQSGSSVPVCIGQQAVLLGNSSTPTLTASQAQMYLRAQMAQQTNLVQVARSLGRAVPLSSQLIFTPTASVTAIQPEAPAPSINTLPTSGQVQNLALRSQQGALTSTLSQSQLQSLSVKQSVASVTGQSVARLKGLGAEPSPQGTAAKASPAETSSETTAKNDKTSDLTTSVCPSVTSVAGHPFISTAYTQIQTHQLLQQHKQQFVIQQQPQILQRGQAQLLEAAAIHPHTVQAVAIQSALPAQPQQCPIPLLPKVPVTCQQATIFHSTTVSQQALAQNGQAHILTNTKAPPLQLTAVKCQIHPVQIQQGVASQNVPDKDTPVLLEPQLLSIPIQEPTRTELRQSDKESQVKENTQGSVNVKAGVGSPPAMTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	XM_009296803.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296803.2

ORF Insert Sequence:

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phc2a ( XM_009296802.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295077.1 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.

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CloneID	ODa27687
Clone ID Related Accession (Same CDS sequence)	XM_009296803.2 , XM_009296802.2 , XM_005162144.3 , NM_152958.2 , XM_005162144.4
Accession Version	XM_009296802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	polyhomeotic-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295077.1
CDS	389..2872
Translation	MEKEQEQGSVASSASVTIPSTTSVSTSTSAGTLSNSSSRQQTVPQISVYGGITDRQTVQVIQQALNRQPSTVAAQYLQQMYAAQQQHLMLQTAALQQQHLSLAAVQQASIVAGRQSCSQHGSTSQQTVASQTTINLATSPAAAHLISRAQSGSSVPVCIGQQAVLLGNSSTPTLTASQAQMYLRAQMAQQTNLVQVARSLGRAVPLSSQLIFTPTASVTAIQPEAPAPSINTLPTSGQVQNLALRSQQGALTSTLSQSQLQSLSVKQSVASVTGQSVARLKGLGAEPSPQGTAAKASPAETSSETTAKNDKTSDLTTSVCPSVTSVAGHPFISTAYTQIQTHQLLQQHKQQFVIQQQPQILQRGQAQLLEAAAIHPHTVQAVAIQSALPAQPQQCPIPLLPKVPVTCQQATIFHSTTVSQQALAQNGQAHILTNTKAPPLQLTAVKCQIHPVQIQQGVASQNVPDKDTPVLLEPQLLSIPIQEPTRTELRQSDKESQVKENTQGSVNVKAGVGSPPAMTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	XM_009296802.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296802.2

ORF Insert Sequence:

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phc2a ( XM_005162144.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162201.1 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27687
Clone ID Related Accession (Same CDS sequence)	XM_009296803.2 , XM_009296802.2 , XM_005162144.3 , NM_152958.2 , XM_005162144.4
Accession Version	XM_005162144.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	polyhomeotic-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162201.1
CDS	392..2875
Translation	MEKEQEQGSVASSASVTIPSTTSVSTSTSAGTLSNSSSRQQTVPQISVYGGITDRQTVQVIQQALNRQPSTVAAQYLQQMYAAQQQHLMLQTAALQQQHLSLAAVQQASIVAGRQSCSQHGSTSQQTVASQTTINLATSPAAAHLISRAQSGSSVPVCIGQQAVLLGNSSTPTLTASQAQMYLRAQMAQQTNLVQVARSLGRAVPLSSQLIFTPTASVTAIQPEAPAPSINTLPTSGQVQNLALRSQQGALTSTLSQSQLQSLSVKQSVASVTGQSVARLKGLGAEPSPQGTAAKASPAETSSETTAKNDKTSDLTTSVCPSVTSVAGHPFISTAYTQIQTHQLLQQHKQQFVIQQQPQILQRGQAQLLEAAAIHPHTVQAVAIQSALPAQPQQCPIPLLPKVPVTCQQATIFHSTTVSQQALAQNGQAHILTNTKAPPLQLTAVKCQIHPVQIQQGVASQNVPDKDTPVLLEPQLLSIPIQEPTRTELRQSDKESQVKENTQGSVNVKAGVGSPPAMTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	XM_005162144.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162144.3

ORF Insert Sequence:

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phc2a ( XM_005162144.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162201.1 Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.

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CloneID	ODa27687
Clone ID Related Accession (Same CDS sequence)	XM_009296803.2 , XM_009296802.2 , XM_005162144.3 , NM_152958.2 , XM_005162144.4
Accession Version	XM_005162144.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	polyhomeotic-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162201.1
CDS	402..2885
Translation	MEKEQEQGSVASSASVTIPSTTSVSTSTSAGTLSNSSSRQQTVPQISVYGGITDRQTVQVIQQALNRQPSTVAAQYLQQMYAAQQQHLMLQTAALQQQHLSLAAVQQASIVAGRQSCSQHGSTSQQTVASQTTINLATSPAAAHLISRAQSGSSVPVCIGQQAVLLGNSSTPTLTASQAQMYLRAQMAQQTNLVQVARSLGRAVPLSSQLIFTPTASVTAIQPEAPAPSINTLPTSGQVQNLALRSQQGALTSTLSQSQLQSLSVKQSVASVTGQSVARLKGLGAEPSPQGTAAKASPAETSSETTAKNDKTSDLTTSVCPSVTSVAGHPFISTAYTQIQTHQLLQQHKQQFVIQQQPQILQRGQAQLLEAAAIHPHTVQAVAIQSALPAQPQQCPIPLLPKVPVTCQQATIFHSTTVSQQALAQNGQAHILTNTKAPPLQLTAVKCQIHPVQIQQGVASQNVPDKDTPVLLEPQLLSIPIQEPTRTELRQSDKESQVKENTQGSVNVKAGVGSPPAMTSGSGNNAPTVTGSAPQNGESKPPPQAVVKPQILTHVIEGFVIQEGAEPFPVERPSLLIENLKQKQHHAYSDLQKHNADSEMEDLSLQELNNQPEPVRTCEFCGNVDFAFNFKRSKRFCSTVCAKRYNVGCTKRMGLFPGKSSPEDTKKPKASDESPKNCSTETRKRNPSIQTTTGASLLSPHPSHPSHGESSQCSDMSSYEEPISPLSNSSFGAPIEHEESFDHSRELTPLLTQHFLASDPTKWNVEDVYEFICSLPGCHEIAEEFRSQEIDGQALMLLKEDHLMSTMNIKLGPALKIFARISMLKDS

Target ORF information:

RefSeq Version	XM_005162144.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polyhomeotic homolog 2a (Drosophila) (phc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162144.4

ORF Insert Sequence:

ATGGAAAAGG AGCAGGAGCA GGGCAGCGTG GCCTCCAGTG CCAGTGTCAC CATCCCCAGC 
ACTACCTCAG TCTCCACCTC AACCTCCGCT GGCACCTTAT CAAACTCCTC CAGCAGACAG 
CAGACTGTGC CTCAGATATC AGTCTATGGA GGAATCACAG ACAGGCAGAC AGTTCAGGTC 
ATCCAGCAGG CCCTGAACAG ACAGCCCAGC ACAGTAGCAG CTCAGTATCT CCAGCAGATG 
TATGCAGCTC AACAACAACA CCTCATGCTC CAGACAGCAG CACTACAGCA ACAGCACCTG 
AGTCTCGCTG CAGTGCAGCA GGCTAGTATA GTGGCAGGAA GACAAAGCTG TAGCCAGCAT 
GGCAGTACGT CCCAGCAGAC TGTGGCCTCC CAAACCACTA TAAACCTGGC CACATCCCCA 
GCGGCAGCTC ATTTGATCAG TCGAGCCCAG AGTGGGAGCT CTGTTCCAGT CTGTATTGGC 
CAACAGGCTG TGCTGCTGGG AAACTCCTCC ACACCCACAC TCACCGCCAG CCAAGCACAG 
ATGTATTTAC GAGCTCAAAT GGCACAGCAA ACTAATCTTG TGCAAGTAGC AAGAAGCCTA 
GGACGAGCTG TTCCTTTGTC GTCCCAACTT ATATTTACAC CAACTGCCTC AGTCACAGCA 
ATTCAACCGG AGGCTCCTGC ACCCTCAATC AACACGCTAC CAACCAGCGG ACAGGTGCAG 
AACTTGGCTC TTCGTAGCCA GCAGGGGGCG CTGACATCCA CCCTCTCTCA GTCTCAGTTA 
CAGAGCCTGA GTGTGAAACA GTCTGTGGCA TCTGTGACTG GCCAATCAGT GGCCCGACTG 
AAAGGCCTAG GAGCAGAGCC GAGCCCTCAG GGAACTGCTG CAAAGGCCAG CCCTGCAGAA 
ACGTCATCAG AAACGACGGC AAAAAATGAC AAAACCAGTG ACCTCACAAC AAGCGTGTGT 
CCCAGTGTGA CCTCGGTGGC AGGACATCCT TTCATCAGCA CAGCATATAC CCAAATCCAG 
ACCCATCAGC TCCTGCAGCA GCACAAGCAG CAGTTTGTGA TTCAGCAGCA GCCTCAGATC 
TTACAGAGAG GTCAGGCTCA GCTCTTAGAG GCCGCCGCCA TTCATCCCCA TACCGTGCAA 
GCTGTGGCCA TTCAGTCTGC CCTGCCAGCA CAGCCTCAGC AGTGTCCAAT CCCTTTGCTG 
CCCAAAGTGC CTGTTACCTG CCAACAAGCC ACCATCTTCC ACTCAACCAC AGTGAGTCAG 
CAGGCACTTG CTCAGAATGG ACAGGCGCAC ATACTGACCA ACACCAAAGC ACCACCTCTA 
CAGCTCACTG CAGTCAAGTG CCAGATCCAT CCTGTTCAAA TTCAACAAGG AGTAGCCTCA 
CAGAATGTTC CTGATAAAGA CACTCCTGTT CTTCTCGAAC CACAGCTGCT ATCCATACCA 
ATACAAGAGC CCACTCGGAC TGAACTACGC CAATCAGACA AAGAAAGTCA AGTGAAAGAA 
AACACACAAG GCAGTGTGAA TGTTAAGGCA GGTGTGGGTT CACCTCCAGC CATGACCTCG 
GGCAGCGGTA ATAATGCGCC CACGGTGACC GGCAGCGCTC CGCAAAATGG CGAGAGTAAA 
CCGCCGCCAC AGGCTGTAGT GAAGCCCCAG ATCCTCACTC ATGTCATCGA GGGATTCGTG 
ATTCAGGAGG GAGCGGAGCC TTTTCCCGTT GAACGTCCGT CCTTGCTGAT CGAGAATTTA 
AAACAGAAGC AGCATCATGC TTATTCGGAT CTACAGAAGC ACAACGCAGA CTCTGAGATG 
GAGGATCTGT CTCTGCAAGA GCTGAATAAT CAACCCGAGC CAGTGCGGAC ATGTGAATTC 
TGTGGGAATG TAGACTTCGC ATTCAACTTC AAGAGATCCA AGCGGTTTTG CTCTACAGTG 
TGTGCGAAGA GATATAATGT TGGATGCACA AAGCGAATGG GCCTTTTTCC AGGGAAATCC 
AGCCCGGAGG ACACAAAGAA ACCAAAAGCC TCAGATGAAA GTCCTAAAAA CTGCAGCACA 
GAAACCAGGA AACGGAATCC AAGCATACAA ACGACCACAG GAGCTTCTTT GTTGTCCCCA 
CATCCATCTC ATCCAAGTCA TGGTGAGTCC AGCCAGTGCT CAGACATGTC CAGCTATGAA 
GAGCCCATCT CACCACTGTC CAACTCGAGC TTTGGAGCCC CGATTGAGCA CGAGGAAAGT 
TTTGATCACA GCAGAGAGCT TACTCCTCTA CTCACGCAGC ACTTCCTGGC CAGCGACCCC 
ACAAAGTGGA ACGTCGAGGA TGTTTATGAA TTCATCTGCT CTCTGCCAGG TTGCCATGAG 
ATCGCTGAAG AGTTTCGCTC TCAGGAGATT GATGGCCAGG CCTTGATGCT CCTCAAAGAG 
GATCATCTCA TGAGCACAAT GAACATCAAA CTGGGCCCTG CACTGAAGAT CTTCGCCCGC 
ATCAGTATGC TCAAGGACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Phenotypic chemical screening using a zebrafish neural crest EMT reporter identifies retinoic acid as an inhibitor of epithelial morphogenesis.
Disease models & mechanisms9(4)389-400(2016 Apr)
Jimenez L,Wang J,Morrison MA,Whatcott C,Soh KK,Warner S,Bearss D,Jette CA,Stewart RA

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Genome research21(8)1328-38(2011 Aug)
Aanes H,Winata CL,Lin CH,Chen JP,Srinivasan KG,Lee SG,Lim AY,Hajan HS,Collas P,Bourque G,Gong Z,Korzh V,Alestr?m P,Mathavan S

The zebrafish genes encoding the Polycomb repressive complex (PRC) 1.
Gene475(1)10-21(2011 Apr)
Le Faou P,V?lkel P,Angrand PO

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phc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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