gnat2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnat2
Entrez Gene ID	140429
Full Name	guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
General protein information	Preferred Names guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 Names guanine nucleotide-binding protein G(t) subunit alpha-2 GNAT3 Galpha t2 T alpha C Tc alpha cone transducin alpha subunit no optokinetic response f nof transducin alpha (cone)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131869.2	NP_571944.1	guanine nucleotide-binding protein G(t) subunit alpha-2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAT2	XP_524787.2
Rattus norvegicus (Norway rat)	Gnat2	NP_001102420.2
Canis lupus familiaris (dog)	GNAT2	XP_547240.2
Mus musculus (house mouse)	Gnat2	NP_032167.1
Gallus gallus (chicken)	GNAT2	NP_990021.1
Xenopus tropicalis (tropical clawed frog)	LOC100216126	NP_001135577.1
Homo sapiens (human)	GNAT2	NP_005263.1
Bos taurus (cattle)	GNAT2	NP_776751.1
	GNAT2	XP_001091580.1
Danio rerio (zebrafish)	gnat2	NP_571944.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors.
Ojeda Naharros I, Gesemann M, Mateos JM, Barmettler G, Forbes A, Ziegler U, Neuhauss SCF, Bachmann-Gagescu R
PLoS genetics13(12)e1007150(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study describes several functional differences between transducin gene duplicates resulting from the teleost-specific tetraploidisation.
Title: Transducin duplicates in the zebrafish retina and pineal complex: differential specialisation after the teleost tetraploidisation.

identifed cis-regulatory elements in upstream promoter region of the TalphaC gene; found PRE-1 is a novel cis-regulatory module that is sufficient to enhance initiation of photoreceptor-specific gene expression in differentiating rod and cone photoreceptors
Title: PRE-1, a cis element sufficient to enhance cone- and rod- specific expression in differentiating zebrafish photoreceptors.

analysis of an evolutionarily conserved enhancer of cone photoreceptor-specific expression
Title: A novel, evolutionarily conserved enhancer of cone photoreceptor-specific expression.

Ca2+ also regulates the extent of phosphorylation of unbleached cone pigments. Immunocytochemical analyses revealed that neither light nor cytoplasmic Ca2+ influences the localization of transducin in zebrafish cones.
Title: Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones.

The following gnat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01933	NM_131869.2 Latest version!	Danio rerio guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 (gnat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnat2 ( NM_131869.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571944.1 Danio rerio guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 (gnat2), mRNA.

Price & Availability↑

CloneID	ODa01933
Clone ID Related Accession (Same CDS sequence)	NM_131869.2
Accession Version	NM_131869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide-binding protein G(t) subunit alpha-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059498.1. On or before Jul 6, 2005 this sequence version replaced XM_684935.1, XM_704279.1, NM_131869.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY050500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505378, SAMEA3505381 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAGCG GAGCGAGCGC AGAGGATAAG GAAATGGCCA AGAAGTCCAA AGAGCTGGAA 
AAGCAGCTCC AGGAAGATGC CGATAAGGAA GCCAAGACGG TCAAACTTCT GCTGCTGGGT 
GCTGGTGAAT CAGGGAAGAG CACCATTGTA AAACAGATGA AGATTCTCCA TCAAGGTGGT 
TATACAAAAG AAGAACAAAT GGAGTTTCGA TCTATTATTT TTGGCAACAT CCTGCAGTCT 
GCTCTGGCCA TCATCAGGGG CATGGAGATG CTGTCAATCA ACTTTGGATC GCCATCTGCA 
CAGGAGGATA GTCAAAAGCT CCAGAACCTG TCTGACTCCA TTGAGGAGGG CACCATGCCT 
CCAGAGCTGG CAGACGTCAT CAAAAGGCTA TGGAAGGATG CAGGCGTGCA GGCCTCTTTT 
GATAGAGCTG CCGAGTACCA GCTGAACGAC TCTGCTGGAT ACTACTTGAA CGAAATGGAC 
AGAATCTGCA AACCTGACTA CCTTCCCACC GAGCAGGATG TGCTGAGATC TCGAGTCAAG 
ACCACTGGTA TCATTGAGGA ACAGTTCGGT TGCAAAGAGC TCCACTTTAG GATGTTTGAT 
GTGGGTGGCC AGAGGTCCGA GAGGAAGAAA TGGATTCATT GTTTCGAAGG TGTGACTTGC 
ATCATCTTCT GTGGAGCTCT TAGCGCTTAT GACATGGTGC TGGTAGAAGA TGATGAAGTG 
AACCGCATGC ACGAGAGTCT CCATCTCTTC AACAGTATCT GCAATCACAG ATTCTTCGCC 
ACAACTTCCA TTGTGCTTTT CCTCAACAAG AAAGATCTCT TCCAGGAGAA GATTAAGAAG 
GTCCACCTGA GCATCTGCTT CCCTGACTAT GATGGTCCAA ACACATATGA AGATGCAAGC 
AACTACATCA AGACTCAATT TCAAGACCTG AACATGAAGA AGGGAGTGAA GGAGATCTAC 
TCACACATGA CCTGTGCCAC GGACACAAAG AACGTCGAGA TTGTGTTTAA CGCCGTGACA 
GACATTATCA TCAAAGAAAA CCTTAAGGAC TGCGGTCTGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571944.1
CDS	185..1249
Translation	MGSGASAEDKEMAKKSKELEKQLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKILHQGGYTKEEQMEFRSIIFGNILQSALAIIRGMEMLSINFGSPSAQEDSQKLQNLSDSIEEGTMPPELADVIKRLWKDAGVQASFDRAAEYQLNDSAGYYLNEMDRICKPDYLPTEQDVLRSRVKTTGIIEEQFGCKELHFRMFDVGGQRSERKKWIHCFEGVTCIIFCGALSAYDMVLVEDDEVNRMHESLHLFNSICNHRFFATTSIVLFLNKKDLFQEKIKKVHLSICFPDYDGPNTYEDASNYIKTQFQDLNMKKGVKEIYSHMTCATDTKNVEIVFNAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_131869.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 (gnat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131869.2

ORF Insert Sequence:

ATGGGTAGCG GAGCGAGCGC AGAGGATAAG GAAATGGCCA AGAAGTCCAA AGAGCTGGAA 
AAGCAGCTCC AGGAAGATGC CGATAAGGAA GCCAAGACGG TCAAACTTCT GCTGCTGGGT 
GCTGGTGAAT CAGGGAAGAG CACCATTGTA AAACAGATGA AGATTCTCCA TCAAGGTGGT 
TATACAAAAG AAGAACAAAT GGAGTTTCGA TCTATTATTT TTGGCAACAT CCTGCAGTCT 
GCTCTGGCCA TCATCAGGGG CATGGAGATG CTGTCAATCA ACTTTGGATC GCCATCTGCA 
CAGGAGGATA GTCAAAAGCT CCAGAACCTG TCTGACTCCA TTGAGGAGGG CACCATGCCT 
CCAGAGCTGG CAGACGTCAT CAAAAGGCTA TGGAAGGATG CAGGCGTGCA GGCCTCTTTT 
GATAGAGCTG CCGAGTACCA GCTGAACGAC TCTGCTGGAT ACTACTTGAA CGAAATGGAC 
AGAATCTGCA AACCTGACTA CCTTCCCACC GAGCAGGATG TGCTGAGATC TCGAGTCAAG 
ACCACTGGTA TCATTGAGGA ACAGTTCGGT TGCAAAGAGC TCCACTTTAG GATGTTTGAT 
GTGGGTGGCC AGAGGTCCGA GAGGAAGAAA TGGATTCATT GTTTCGAAGG TGTGACTTGC 
ATCATCTTCT GTGGAGCTCT TAGCGCTTAT GACATGGTGC TGGTAGAAGA TGATGAAGTG 
AACCGCATGC ACGAGAGTCT CCATCTCTTC AACAGTATCT GCAATCACAG ATTCTTCGCC 
ACAACTTCCA TTGTGCTTTT CCTCAACAAG AAAGATCTCT TCCAGGAGAA GATTAAGAAG 
GTCCACCTGA GCATCTGCTT CCCTGACTAT GATGGTCCAA ACACATATGA AGATGCAAGC 
AACTACATCA AGACTCAATT TCAAGACCTG AACATGAAGA AGGGAGTGAA GGAGATCTAC 
TCACACATGA CCTGTGCCAC GGACACAAAG AACGTCGAGA TTGTGTTTAA CGCCGTGACA 
GACATTATCA TCAAAGAAAA CCTTAAGGAC TGCGGTCTGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors.
PLoS genetics13(12)e1007150(2017 12)
Ojeda Naharros I,Gesemann M,Mateos JM,Barmettler G,Forbes A,Ziegler U,Neuhauss SCF,Bachmann-Gagescu R

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gnat2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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