ube2ia cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube2ia
Entrez Gene ID	114445
Full Name	ubiquitin-conjugating enzyme E2Ia
Synonyms	Ubc9-2,ube2i2,ube2ib
General protein information	Preferred Names ubiquitin-conjugating enzyme E2Ia Names SUMO-conjugating enzyme UBC9-B RING-type E3 SUMO transferase UBC9-B SUMO-protein ligase B ubiquitin carrier protein 9-B ubiquitin carrier protein I-B ubiquitin-conjugating enzyme E2 I-B ubiquitin-conjugating enzyme E2I2 ubiquitin-protein ligase I-B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469272.1	XP_021324947.1	SUMO-conjugating enzyme UBC9-B isoform X1
NM_131833.2	NP_571908.1	SUMO-conjugating enzyme UBC9-B

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2I	NP_003336.1
Pan troglodytes (chimpanzee)	UBE2I	XP_003314955.1
	UBE2I	XP_002802397.1
Bos taurus (cattle)	UBE2I	NP_001092842.1
Rattus norvegicus (Norway rat)	Ube2i	NP_037182.1
Gallus gallus (chicken)	UBE2I	NP_989596.1
Saccharomyces cerevisiae (baker's yeast)	UBC9	NP_010219.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011076	XP_309574.1
Oryza sativa (rice)	Os03g0123100	NP_001048802.1
Oryza sativa (rice)	Os10g0536000	NP_001065167.1
Mus musculus (house mouse)	Ube2i	NP_035795.1
Danio rerio (zebrafish)	ube2i2	NP_571908.1
Eremothecium gossypii	AGOS_AER056C	NP_984916.1
Schizosaccharomyces pombe (fission yeast)	hus5	NP_593204.1
Arabidopsis thaliana (thale cress)	SCE1	NP_191346.1
Xenopus tropicalis (tropical clawed frog)	ube2i	NP_001016408.1
Drosophila melanogaster (fruit fly)	lwr	NP_001259833.1
Kluyveromyces lactis	KLLA0E05039g	XP_454172.1
Magnaporthe oryzae (rice blast fungus)	MGG_00970	XP_003717980.1
Canis lupus familiaris (dog)	UBE2I	XP_005621791.1
Neurospora crassa	NCU04302	XP_961092.1

Gene Ontology
Bibliography

Related articles in PubMed

GATA5 SUMOylation is indispensable for zebrafish cardiac development.
Wen B, Yuan H, Liu X, Wang H, Chen S, Chen Z, de The H, Zhou J, Zhu J
Biochimica et biophysica acta1861(7)1691-1701(2017 07)

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Lei L, Yan SY, Yang R, Chen JY, Li Y, Bu Y, Chang N, Zhou Q, Zhu X, Li CY, Xiong JW
Nucleic acids research45(6)3422-3436(2017 Apr)

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation.
Pagano C, di Martino O, Ruggiero G, Maria Guarino A, Mueller N, Siauciunaite R, Reischl M, Simon Foulkes N, Vallone D, Calabr? V
Oncotarget8(4)6193-6205(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutations in the zinc-binding domain of Apobec2 diminish its ability to stimulate Pou6f2 binding to DNA, and knockdown of Ubc9 or Pou6f2 suppressed retina regeneration
Title: Zinc-binding domain-dependent, deaminase-independent actions of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide 2 (Apobec2), mediate its effect on zebrafish retina regeneration.

The following ube2ia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube2ia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01069	NM_131833.2 Latest version!	Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa141542	XM_021469272.1 Latest version!	Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube2ia ( NM_131833.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571908.1 Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), mRNA.

Price & Availability↑

CloneID	ODa01069
Clone ID Related Accession (Same CDS sequence)	NM_131833.2
Accession Version	NM_131833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-23
Organism	Danio rerio(zebrafish)
Product	SUMO-conjugating enzyme UBC9-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058302.1. On May 16, 2004 this sequence version replaced NM_131833.1. ##Evidence-Data-START## Transcript exon combination :: BC058302.1, EH451253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGTA TAGCATTGAG TCGTCTCGCA CAAGAACGAA AGGCTTGGCG GAAAGACCAT 
CCTTTTGGGT TTGTAGCCGT ACCAACAAAA AACCCTGATG GAACCATGAA CTTAATGAAC 
TGGGAGTGTG CCATACCAGG AAAAAAAGGG ACACCGTGGG AGGGAGGCTT GTTCAAACTT 
CGGATGCTTT TCAAGGACGA CTACCCTTCC TCTCCACCAA AGTGTAAATT TGAGCCTCCT 
TTATTCCATC CGAATGTATA CCCGTCTGGA ACCGTATGCT TGTCTATTTT AGAGGAGGAC 
AAAGATTGGA GGCCTGCCAT TACGATCAAG CAGATCTTGC TAGGAATCCA AGAGCTCCTA 
AATGAACCAA ATATTCAGGA TCCCGCACAG GCCGAGGCAT ACACGATTTA CTGCCAAAAC 
AGAGTGGAGT ATGAAAAAAG GGTCCGAGCA CAAGCCAAAA AGTTCTCTCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571908.1
CDS	159..632
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSP

Target ORF information:

RefSeq Version	NM_131833.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131833.2

ORF Insert Sequence:

ATGTCTGGTA TAGCATTGAG TCGTCTCGCA CAAGAACGAA AGGCTTGGCG GAAAGACCAT 
CCTTTTGGGT TTGTAGCCGT ACCAACAAAA AACCCTGATG GAACCATGAA CTTAATGAAC 
TGGGAGTGTG CCATACCAGG AAAAAAAGGG ACACCGTGGG AGGGAGGCTT GTTCAAACTT 
CGGATGCTTT TCAAGGACGA CTACCCTTCC TCTCCACCAA AGTGTAAATT TGAGCCTCCT 
TTATTCCATC CGAATGTATA CCCGTCTGGA ACCGTATGCT TGTCTATTTT AGAGGAGGAC 
AAAGATTGGA GGCCTGCCAT TACGATCAAG CAGATCTTGC TAGGAATCCA AGAGCTCCTA 
AATGAACCAA ATATTCAGGA TCCCGCACAG GCCGAGGCAT ACACGATTTA CTGCCAAAAC 
AGAGTGGAGT ATGAAAAAAG GGTCCGAGCA CAAGCCAAAA AGTTCTCTCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube2ia ( XM_021469272.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324947.1 Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141542
Clone ID Related Accession (Same CDS sequence)	XM_021469272.1
Accession Version	XM_021469272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SUMO-conjugating enzyme UBC9-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTAA TGAACTGGGA GTGTGCCATA CCAGGGAAAA AAGGGACACC GTGGGAGGGA 
GGCTTGTTCA AACTTCGGAT GCTTTTCAAG GACGACTACC CTTCCTCTCC ACCAAAGTGT 
AAATTTGAGC CTCCTTTATT CCATCCGAAT GTATACCCGT CTGGTACCGT ATGCTTGTCT 
ATTTTAGAGG AGGACAAAGA TTGGAGGCCT GCCATTACGA TCAAGCAGAT CTTGCTAGGA 
ATCCAAGAGC TCCTAAATGA ACCAAATATT CAGGATCCCG CACAGGCCGA GGCATACACG 
ATTTACTGCC AAAACAGAGT GGAGTATGAA AAAAGGGTCC GAGCACAAGC CAAAAAGTTC 
TCTCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324947.1
CDS	201..569
Translation	MNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSP

Target ORF information:

RefSeq Version	XM_021469272.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-conjugating enzyme E2Ia (ube2ia), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469272.1

ORF Insert Sequence:

ATGAACTTAA TGAACTGGGA GTGTGCCATA CCAGGGAAAA AAGGGACACC GTGGGAGGGA 
GGCTTGTTCA AACTTCGGAT GCTTTTCAAG GACGACTACC CTTCCTCTCC ACCAAAGTGT 
AAATTTGAGC CTCCTTTATT CCATCCGAAT GTATACCCGT CTGGTACCGT ATGCTTGTCT 
ATTTTAGAGG AGGACAAAGA TTGGAGGCCT GCCATTACGA TCAAGCAGAT CTTGCTAGGA 
ATCCAAGAGC TCCTAAATGA ACCAAATATT CAGGATCCCG CACAGGCCGA GGCATACACG 
ATTTACTGCC AAAACAGAGT GGAGTATGAA AAAAGGGTCC GAGCACAAGC CAAAAAGTTC 
TCTCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GATA5 SUMOylation is indispensable for zebrafish cardiac development.
Biochimica et biophysica acta1861(7)1691-1701(2017 07)
Wen B,Yuan H,Liu X,Wang H,Chen S,Chen Z,de The H,Zhou J,Zhu J

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Nucleic acids research45(6)3422-3436(2017 Apr)
Lei L,Yan SY,Yang R,Chen JY,Li Y,Bu Y,Chang N,Zhou Q,Zhu X,Li CY,Xiong JW

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation.
Oncotarget8(4)6193-6205(2017 Jan)
Pagano C,di Martino O,Ruggiero G,Maria Guarino A,Mueller N,Siauciunaite R,Reischl M,Simon Foulkes N,Vallone D,Calabr? V

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ube2ia cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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