rapgef3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef3
Entrez Gene ID	101886094
Full Name	Rap guanine nucleotide exchange factor (GEF) 3
Synonyms	si:dkey-27m20.2
General protein information	Preferred Names Rap guanine nucleotide exchange factor (GEF) 3 Names rap guanine nucleotide exchange factor 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162353.4	XP_005162410.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_009297024.3	XP_009295299.1	rap guanine nucleotide exchange factor 3 isoform X3
XM_005162353.3	XP_005162410.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_009297025.2	XP_009295300.1	rap guanine nucleotide exchange factor 3 isoform X4
XM_009297023.3	XP_009295298.1	rap guanine nucleotide exchange factor 3 isoform X2
XM_009297023.2	XP_009295298.1	rap guanine nucleotide exchange factor 3 isoform X2
XM_009297024.2	XP_009295299.1	rap guanine nucleotide exchange factor 3 isoform X3
XM_009297025.3	XP_009295300.1	rap guanine nucleotide exchange factor 3 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF3	XP_001162479.2
Xenopus tropicalis (tropical clawed frog)	rapgef3	XP_002935702.2
Rattus norvegicus (Norway rat)	Rapgef3	NP_067722.1
Danio rerio (zebrafish)	LOC101886094	XP_005162410.1
	RAPGEF3	XP_001098291.2
Gallus gallus (chicken)	RAPGEF3	XP_424494.4
Bos taurus (cattle)	RAPGEF3	NP_001073771.1
Homo sapiens (human)	RAPGEF3	NP_001092001.1
Canis lupus familiaris (dog)	RAPGEF3	NP_001093414.1
Mus musculus (house mouse)	Rapgef3	NP_001171281.1

Gene Ontology

The following rapgef3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27941	XM_005162353.4 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51777	XM_009297023.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51778	XM_009297024.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51779	XM_009297025.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa27941	XM_005162353.3	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51777	XM_009297023.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51778	XM_009297024.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa51779	XM_009297025.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef3 ( XM_005162353.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162410.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27941
Clone ID Related Accession (Same CDS sequence)	XM_005162353.4 , XM_005162353.3
Accession Version	XM_005162353.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162353.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGTGC CTTCATCTCC GCAGAAAGGC TTGACGGAGA GAATGTTCTT GGATGCTCAA 
GCTATCCGAA TATCAACATA TTCAGACCAG TCTCTGAACC TCCGCAGTGC CACCAGTATC 
AGACAGTACA TCCAGAACCT GAAAGTGATC GACAATCAGA AGAAACTAAC TCAGCTCTCC 
AGAGCCTTAG AGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162410.1
CDS	58..2715
Translation	MEEIHARMFSFFKQILSAKKKNRLESPAVMCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_005162353.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162353.4

ORF Insert Sequence:

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGTGC CTTCATCTCC GCAGAAAGGC TTGACGGAGA GAATGTTCTT GGATGCTCAA 
GCTATCCGAA TATCAACATA TTCAGACCAG TCTCTGAACC TCCGCAGTGC CACCAGTATC 
AGACAGTACA TCCAGAACCT GAAAGTGATC GACAATCAGA AGAAACTAAC TCAGCTCTCC 
AGAGCCTTAG AGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_005162353.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162410.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27941
Clone ID Related Accession (Same CDS sequence)	XM_005162353.4 , XM_005162353.3
Accession Version	XM_005162353.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGTGC CTTCATCTCC GCAGAAAGGC TTGACGGAGA GAATGTTCTT GGATGCTCAA 
GCTATCCGAA TATCAACATA TTCAGACCAG TCTCTGAACC TCCGCAGTGC CACCAGTATC 
AGACAGTACA TCCAGAACCT GAAAGTGATC GACAATCAGA AGAAACTAAC TCAGCTCTCC 
AGAGCCTTAG AGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162410.1
CDS	62..2719
Translation	MEEIHARMFSFFKQILSAKKKNRLESPAVMCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_005162353.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162353.3

ORF Insert Sequence:

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGTGC CTTCATCTCC GCAGAAAGGC TTGACGGAGA GAATGTTCTT GGATGCTCAA 
GCTATCCGAA TATCAACATA TTCAGACCAG TCTCTGAACC TCCGCAGTGC CACCAGTATC 
AGACAGTACA TCCAGAACCT GAAAGTGATC GACAATCAGA AGAAACTAAC TCAGCTCTCC 
AGAGCCTTAG AGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297023.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295298.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa51777
Clone ID Related Accession (Same CDS sequence)	XM_009297023.3 , XM_009297023.2
Accession Version	XM_009297023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTCT TTCGGAGTTA CAATTACAAA GTCTTTCCCG ATCGTTGTTC CAATGAGAAA 
ACTCCTCAGA TTCGGGGAAT CAGCTGGACG CCTCTGCCAG ACGCTGTGGA TACACAAGAC 
ACCATCAAAC AGTTTTTATC AGATCGAATC CTGAAGGCTG CACGGGTAGT TTACAGCACT 
CTGATGGAGG GTAACCCAGG CCTGATAAGA GACAGAAAAC ACCATCTTAA GACCTACAGA 
CAGTGCTGCA GCGGAAAGGA GCTTGTGGAT TGGTTAATGA AACTCAATGA TTGTTTCCAG 
TCCCGGAGTC AGGCAGTCGG AATGTGGCAG GTTTTGGTGG ATGAGGGCAT TTTGGGTCAT 
GTAAAACAGG AGTTAAACTT CCATGACAAA GACACACAGT TTTACCGCTT TATGGAAGCA 
GAGTTTGACC TCAATCACAC AACTAACGAA AAAGACTCCA AAGACGACGA ACTACAAGAG 
AGTTTATCGC TACTAATCCA GATGGGCCCA GATGCTCTTT TGACGATGAT ACTGCGTAAA 
TGCCCAAGCC AGAGAACCCC AGAAGATCTG GAAGTTATTT ATGAAGAACT TCTGCACGTC 
AAAGCTGTGG CCCACTTGTC TACATCTGTT CGTAAAGAGC TGGCATCAGT GCTGGTATTT 
GAAAGCCATG CCAAGGCGGG AACAGTCTTG TTCAGTCAGG GGGACAAAGG GACTTCCTGG 
TACATCATCT GGAGAGGCTC TGTTAATGTC ATCACACACG GCAAGGGCTT GGTGACTACG 
CTTCATGAAG GAGATGATTT TGGCCAGTTG GCATTGGTGA ATGACGCTCC TCGAGCCGCC 
ACCATCATTT TAAGAGAGGA CAATTGCCAC TTTCTGCGGG TGGACAAGCA GGATTTTATA 
CGCATTCTTA AGGACGTCGA GGCAAACACC GTCCGTCTGG AGGAGCATGG TAAAGTCGTT 
CTGGTTCTTG AGAAGAGCTC GGCGCAGGAT ACACCAAGCT GCAACAAGTA CACGGTAATG 
TCAGGAACGC CGGAAAAGAT CCTGGAGCAC ATGCTGGAGG CCATAAAACT GGAAACCAAC 
GGAGCTGACT TCATAGATCC CTGTGTGACT GACTTTCTCC TAACGCATCC CGTCTTCATG 
CCCTGCAGTC AACTGTGTGC TGCCCTCCAG CATCATTACC AAGCGGAGCC GTCTGAAGGC 
TCAGACCTGG AGAAGGCAGC GTACGCTCTC AACACCAAGC AGAAGGTGGT GAAGCTGGTG 
TGTCACTGGG TGGCTCTGTT CGCTCTGCTG CTCAGGGACG ATCCTGTGGC CTCTGAATTC 
CTGGAGAAAT TCAGGGAAGG AGTGATGGCA GACTCAAGAC TTTCTAGCAT GCTTAGAGAA 
CAGCTAAGGG ACAGGAGGAA GACAAAAATA ATGGAGAATG GATGTCACAC TCTGACAAAG 
CTGAACCAAA AGTTTGATTG GTTTTCAGCT TACGAAGAGC CGGTGGGAAA ACTGAGGTCG 
ATTAAAGCCC AAGATAAAGT GTTGTATGAG ATCTTTAAAC CGGATCATAA GGCTGTCACT 
GTGATGCTGC CAGTTGATGC ATCGGTGAAC GACATCTTGA CTACATTAGT GGATCCTGAC 
AGAAACTATG TGCTTGTCAA GATGAATTCC TCAGGAGACA GAGTCCAGCT GAAGTTGGAG 
ACCACAGCAG TGTCTGCCTC TTTAGGAGTG AACGAAAAGC TTTTCCTCTG CACTTCCAGC 
CAAGTTGAAC AACTGACACC TGATAAAGAG CAGCTGGGGC CGGAGAAGAG CACCATGGAC 
ACTCTGGAGC AGATTTGCTC CAAGGACATG GCCAGTCAAC ACACCAGCTA CGACTGGGAA 
CTCTTCATGG CCATGCATGA GGTGGAGCTT GTCTACTATG TTTTCGGACG GGAAAAGTTC 
CCTGGATCCA CCACAGCAAA CCTCGAGCGG TTTGTTCGTC GCTTCAATGA GGTTCAGTAC 
TGGGTTGTGA CTGAACTGTG TCTCTGTGAA GACCTGGGCA AGAGAGCCAT ACTGCTGAAG 
AAGTTCATCA AGATGGCTGT TGTTTTGAAA GAGCAGAAGA ACCTCAACTC TTTTTTCGCA 
GTAATGTTTG GCCTCAGCAA CAGTGCAGTG CAGAGGCTCA ATAAAACATG GGAGAGACTT 
CCCAATAAAA CCAAAAGGAT CTACTGTGCA TACGAAAGAC TGATGGATCC GTCCCGTAAC 
CACAGGGCCT ACAGACTGAC TATTGCCAAA CTCAGCCCTC CATACATTCC CTTCATGCCA 
CTGCTTCTAA AAGATATGAC ATTTATTCAT GAGGGAAACA AGAACTACAC TGATAAACTG 
GTCAACTTTG AGAAAATGCG CATGATTGCC AGAACAGTGA AGACAGTTCG GGATTGTCGA 
AGCCAACCTT ACGTGCCTTC ATCTCCGCAG AAAGGCTTGA CGGAGAGAAT GTTCTTGGAT 
GCTCAAGCTA TCCGAATATC AACATATTCA GACCAGTCTC TGAACCTCCG CAGTGCCACC 
AGTATCAGAC AGTACATCCA GAACCTGAAA GTGATCGACA ATCAGAAGAA ACTAACTCAG 
CTCTCCAGAG CCTTAGAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295298.1
CDS	196..2799
Translation	MHLFRSYNYKVFPDRCSNEKTPQIRGISWTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297023.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297023.3

ORF Insert Sequence:

ATGCATCTCT TTCGGAGTTA CAATTACAAA GTCTTTCCCG ATCGTTGTTC CAATGAGAAA 
ACTCCTCAGA TTCGGGGAAT CAGCTGGACG CCTCTGCCAG ACGCTGTGGA TACACAAGAC 
ACCATCAAAC AGTTTTTATC AGATCGAATC CTGAAGGCTG CACGGGTAGT TTACAGCACT 
CTGATGGAGG GTAACCCAGG CCTGATAAGA GACAGAAAAC ACCATCTTAA GACCTACAGA 
CAGTGCTGCA GCGGAAAGGA GCTTGTGGAT TGGTTAATGA AACTCAATGA TTGTTTCCAG 
TCCCGGAGTC AGGCAGTCGG AATGTGGCAG GTTTTGGTGG ATGAGGGCAT TTTGGGTCAT 
GTAAAACAGG AGTTAAACTT CCATGACAAA GACACACAGT TTTACCGCTT TATGGAAGCA 
GAGTTTGACC TCAATCACAC AACTAACGAA AAAGACTCCA AAGACGACGA ACTACAAGAG 
AGTTTATCGC TACTAATCCA GATGGGCCCA GATGCTCTTT TGACGATGAT ACTGCGTAAA 
TGCCCAAGCC AGAGAACCCC AGAAGATCTG GAAGTTATTT ATGAAGAACT TCTGCACGTC 
AAAGCTGTGG CCCACTTGTC TACATCTGTT CGTAAAGAGC TGGCATCAGT GCTGGTATTT 
GAAAGCCATG CCAAGGCGGG AACAGTCTTG TTCAGTCAGG GGGACAAAGG GACTTCCTGG 
TACATCATCT GGAGAGGCTC TGTTAATGTC ATCACACACG GCAAGGGCTT GGTGACTACG 
CTTCATGAAG GAGATGATTT TGGCCAGTTG GCATTGGTGA ATGACGCTCC TCGAGCCGCC 
ACCATCATTT TAAGAGAGGA CAATTGCCAC TTTCTGCGGG TGGACAAGCA GGATTTTATA 
CGCATTCTTA AGGACGTCGA GGCAAACACC GTCCGTCTGG AGGAGCATGG TAAAGTCGTT 
CTGGTTCTTG AGAAGAGCTC GGCGCAGGAT ACACCAAGCT GCAACAAGTA CACGGTAATG 
TCAGGAACGC CGGAAAAGAT CCTGGAGCAC ATGCTGGAGG CCATAAAACT GGAAACCAAC 
GGAGCTGACT TCATAGATCC CTGTGTGACT GACTTTCTCC TAACGCATCC CGTCTTCATG 
CCCTGCAGTC AACTGTGTGC TGCCCTCCAG CATCATTACC AAGCGGAGCC GTCTGAAGGC 
TCAGACCTGG AGAAGGCAGC GTACGCTCTC AACACCAAGC AGAAGGTGGT GAAGCTGGTG 
TGTCACTGGG TGGCTCTGTT CGCTCTGCTG CTCAGGGACG ATCCTGTGGC CTCTGAATTC 
CTGGAGAAAT TCAGGGAAGG AGTGATGGCA GACTCAAGAC TTTCTAGCAT GCTTAGAGAA 
CAGCTAAGGG ACAGGAGGAA GACAAAAATA ATGGAGAATG GATGTCACAC TCTGACAAAG 
CTGAACCAAA AGTTTGATTG GTTTTCAGCT TACGAAGAGC CGGTGGGAAA ACTGAGGTCG 
ATTAAAGCCC AAGATAAAGT GTTGTATGAG ATCTTTAAAC CGGATCATAA GGCTGTCACT 
GTGATGCTGC CAGTTGATGC ATCGGTGAAC GACATCTTGA CTACATTAGT GGATCCTGAC 
AGAAACTATG TGCTTGTCAA GATGAATTCC TCAGGAGACA GAGTCCAGCT GAAGTTGGAG 
ACCACAGCAG TGTCTGCCTC TTTAGGAGTG AACGAAAAGC TTTTCCTCTG CACTTCCAGC 
CAAGTTGAAC AACTGACACC TGATAAAGAG CAGCTGGGGC CGGAGAAGAG CACCATGGAC 
ACTCTGGAGC AGATTTGCTC CAAGGACATG GCCAGTCAAC ACACCAGCTA CGACTGGGAA 
CTCTTCATGG CCATGCATGA GGTGGAGCTT GTCTACTATG TTTTCGGACG GGAAAAGTTC 
CCTGGATCCA CCACAGCAAA CCTCGAGCGG TTTGTTCGTC GCTTCAATGA GGTTCAGTAC 
TGGGTTGTGA CTGAACTGTG TCTCTGTGAA GACCTGGGCA AGAGAGCCAT ACTGCTGAAG 
AAGTTCATCA AGATGGCTGT TGTTTTGAAA GAGCAGAAGA ACCTCAACTC TTTTTTCGCA 
GTAATGTTTG GCCTCAGCAA CAGTGCAGTG CAGAGGCTCA ATAAAACATG GGAGAGACTT 
CCCAATAAAA CCAAAAGGAT CTACTGTGCA TACGAAAGAC TGATGGATCC GTCCCGTAAC 
CACAGGGCCT ACAGACTGAC TATTGCCAAA CTCAGCCCTC CATACATTCC CTTCATGCCA 
CTGCTTCTAA AAGATATGAC ATTTATTCAT GAGGGAAACA AGAACTACAC TGATAAACTG 
GTCAACTTTG AGAAAATGCG CATGATTGCC AGAACAGTGA AGACAGTTCG GGATTGTCGA 
AGCCAACCTT ACGTGCCTTC ATCTCCGCAG AAAGGCTTGA CGGAGAGAAT GTTCTTGGAT 
GCTCAAGCTA TCCGAATATC AACATATTCA GACCAGTCTC TGAACCTCCG CAGTGCCACC 
AGTATCAGAC AGTACATCCA GAACCTGAAA GTGATCGACA ATCAGAAGAA ACTAACTCAG 
CTCTCCAGAG CCTTAGAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297023.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295298.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa51777
Clone ID Related Accession (Same CDS sequence)	XM_009297023.3 , XM_009297023.2
Accession Version	XM_009297023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTCT TTCGGAGTTA CAATTACAAA GTCTTTCCCG ATCGTTGTTC CAATGAGAAA 
ACTCCTCAGA TTCGGGGAAT CAGCTGGACG CCTCTGCCAG ACGCTGTGGA TACACAAGAC 
ACCATCAAAC AGTTTTTATC AGATCGAATC CTGAAGGCTG CACGGGTAGT TTACAGCACT 
CTGATGGAGG GTAACCCAGG CCTGATAAGA GACAGAAAAC ACCATCTTAA GACCTACAGA 
CAGTGCTGCA GCGGAAAGGA GCTTGTGGAT TGGTTAATGA AACTCAATGA TTGTTTCCAG 
TCCCGGAGTC AGGCAGTCGG AATGTGGCAG GTTTTGGTGG ATGAGGGCAT TTTGGGTCAT 
GTAAAACAGG AGTTAAACTT CCATGACAAA GACACACAGT TTTACCGCTT TATGGAAGCA 
GAGTTTGACC TCAATCACAC AACTAACGAA AAAGACTCCA AAGACGACGA ACTACAAGAG 
AGTTTATCGC TACTAATCCA GATGGGCCCA GATGCTCTTT TGACGATGAT ACTGCGTAAA 
TGCCCAAGCC AGAGAACCCC AGAAGATCTG GAAGTTATTT ATGAAGAACT TCTGCACGTC 
AAAGCTGTGG CCCACTTGTC TACATCTGTT CGTAAAGAGC TGGCATCAGT GCTGGTATTT 
GAAAGCCATG CCAAGGCGGG AACAGTCTTG TTCAGTCAGG GGGACAAAGG GACTTCCTGG 
TACATCATCT GGAGAGGCTC TGTTAATGTC ATCACACACG GCAAGGGCTT GGTGACTACG 
CTTCATGAAG GAGATGATTT TGGCCAGTTG GCATTGGTGA ATGACGCTCC TCGAGCCGCC 
ACCATCATTT TAAGAGAGGA CAATTGCCAC TTTCTGCGGG TGGACAAGCA GGATTTTATA 
CGCATTCTTA AGGACGTCGA GGCAAACACC GTCCGTCTGG AGGAGCATGG TAAAGTCGTT 
CTGGTTCTTG AGAAGAGCTC GGCGCAGGAT ACACCAAGCT GCAACAAGTA CACGGTAATG 
TCAGGAACGC CGGAAAAGAT CCTGGAGCAC ATGCTGGAGG CCATAAAACT GGAAACCAAC 
GGAGCTGACT TCATAGATCC CTGTGTGACT GACTTTCTCC TAACGCATCC CGTCTTCATG 
CCCTGCAGTC AACTGTGTGC TGCCCTCCAG CATCATTACC AAGCGGAGCC GTCTGAAGGC 
TCAGACCTGG AGAAGGCAGC GTACGCTCTC AACACCAAGC AGAAGGTGGT GAAGCTGGTG 
TGTCACTGGG TGGCTCTGTT CGCTCTGCTG CTCAGGGACG ATCCTGTGGC CTCTGAATTC 
CTGGAGAAAT TCAGGGAAGG AGTGATGGCA GACTCAAGAC TTTCTAGCAT GCTTAGAGAA 
CAGCTAAGGG ACAGGAGGAA GACAAAAATA ATGGAGAATG GATGTCACAC TCTGACAAAG 
CTGAACCAAA AGTTTGATTG GTTTTCAGCT TACGAAGAGC CGGTGGGAAA ACTGAGGTCG 
ATTAAAGCCC AAGATAAAGT GTTGTATGAG ATCTTTAAAC CGGATCATAA GGCTGTCACT 
GTGATGCTGC CAGTTGATGC ATCGGTGAAC GACATCTTGA CTACATTAGT GGATCCTGAC 
AGAAACTATG TGCTTGTCAA GATGAATTCC TCAGGAGACA GAGTCCAGCT GAAGTTGGAG 
ACCACAGCAG TGTCTGCCTC TTTAGGAGTG AACGAAAAGC TTTTCCTCTG CACTTCCAGC 
CAAGTTGAAC AACTGACACC TGATAAAGAG CAGCTGGGGC CGGAGAAGAG CACCATGGAC 
ACTCTGGAGC AGATTTGCTC CAAGGACATG GCCAGTCAAC ACACCAGCTA CGACTGGGAA 
CTCTTCATGG CCATGCATGA GGTGGAGCTT GTCTACTATG TTTTCGGACG GGAAAAGTTC 
CCTGGATCCA CCACAGCAAA CCTCGAGCGG TTTGTTCGTC GCTTCAATGA GGTTCAGTAC 
TGGGTTGTGA CTGAACTGTG TCTCTGTGAA GACCTGGGCA AGAGAGCCAT ACTGCTGAAG 
AAGTTCATCA AGATGGCTGT TGTTTTGAAA GAGCAGAAGA ACCTCAACTC TTTTTTCGCA 
GTAATGTTTG GCCTCAGCAA CAGTGCAGTG CAGAGGCTCA ATAAAACATG GGAGAGACTT 
CCCAATAAAA CCAAAAGGAT CTACTGTGCA TACGAAAGAC TGATGGATCC GTCCCGTAAC 
CACAGGGCCT ACAGACTGAC TATTGCCAAA CTCAGCCCTC CATACATTCC CTTCATGCCA 
CTGCTTCTAA AAGATATGAC ATTTATTCAT GAGGGAAACA AGAACTACAC TGATAAACTG 
GTCAACTTTG AGAAAATGCG CATGATTGCC AGAACAGTGA AGACAGTTCG GGATTGTCGA 
AGCCAACCTT ACGTGCCTTC ATCTCCGCAG AAAGGCTTGA CGGAGAGAAT GTTCTTGGAT 
GCTCAAGCTA TCCGAATATC AACATATTCA GACCAGTCTC TGAACCTCCG CAGTGCCACC 
AGTATCAGAC AGTACATCCA GAACCTGAAA GTGATCGACA ATCAGAAGAA ACTAACTCAG 
CTCTCCAGAG CCTTAGAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295298.1
CDS	199..2802
Translation	MHLFRSYNYKVFPDRCSNEKTPQIRGISWTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297023.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297023.2

ORF Insert Sequence:

ATGCATCTCT TTCGGAGTTA CAATTACAAA GTCTTTCCCG ATCGTTGTTC CAATGAGAAA 
ACTCCTCAGA TTCGGGGAAT CAGCTGGACG CCTCTGCCAG ACGCTGTGGA TACACAAGAC 
ACCATCAAAC AGTTTTTATC AGATCGAATC CTGAAGGCTG CACGGGTAGT TTACAGCACT 
CTGATGGAGG GTAACCCAGG CCTGATAAGA GACAGAAAAC ACCATCTTAA GACCTACAGA 
CAGTGCTGCA GCGGAAAGGA GCTTGTGGAT TGGTTAATGA AACTCAATGA TTGTTTCCAG 
TCCCGGAGTC AGGCAGTCGG AATGTGGCAG GTTTTGGTGG ATGAGGGCAT TTTGGGTCAT 
GTAAAACAGG AGTTAAACTT CCATGACAAA GACACACAGT TTTACCGCTT TATGGAAGCA 
GAGTTTGACC TCAATCACAC AACTAACGAA AAAGACTCCA AAGACGACGA ACTACAAGAG 
AGTTTATCGC TACTAATCCA GATGGGCCCA GATGCTCTTT TGACGATGAT ACTGCGTAAA 
TGCCCAAGCC AGAGAACCCC AGAAGATCTG GAAGTTATTT ATGAAGAACT TCTGCACGTC 
AAAGCTGTGG CCCACTTGTC TACATCTGTT CGTAAAGAGC TGGCATCAGT GCTGGTATTT 
GAAAGCCATG CCAAGGCGGG AACAGTCTTG TTCAGTCAGG GGGACAAAGG GACTTCCTGG 
TACATCATCT GGAGAGGCTC TGTTAATGTC ATCACACACG GCAAGGGCTT GGTGACTACG 
CTTCATGAAG GAGATGATTT TGGCCAGTTG GCATTGGTGA ATGACGCTCC TCGAGCCGCC 
ACCATCATTT TAAGAGAGGA CAATTGCCAC TTTCTGCGGG TGGACAAGCA GGATTTTATA 
CGCATTCTTA AGGACGTCGA GGCAAACACC GTCCGTCTGG AGGAGCATGG TAAAGTCGTT 
CTGGTTCTTG AGAAGAGCTC GGCGCAGGAT ACACCAAGCT GCAACAAGTA CACGGTAATG 
TCAGGAACGC CGGAAAAGAT CCTGGAGCAC ATGCTGGAGG CCATAAAACT GGAAACCAAC 
GGAGCTGACT TCATAGATCC CTGTGTGACT GACTTTCTCC TAACGCATCC CGTCTTCATG 
CCCTGCAGTC AACTGTGTGC TGCCCTCCAG CATCATTACC AAGCGGAGCC GTCTGAAGGC 
TCAGACCTGG AGAAGGCAGC GTACGCTCTC AACACCAAGC AGAAGGTGGT GAAGCTGGTG 
TGTCACTGGG TGGCTCTGTT CGCTCTGCTG CTCAGGGACG ATCCTGTGGC CTCTGAATTC 
CTGGAGAAAT TCAGGGAAGG AGTGATGGCA GACTCAAGAC TTTCTAGCAT GCTTAGAGAA 
CAGCTAAGGG ACAGGAGGAA GACAAAAATA ATGGAGAATG GATGTCACAC TCTGACAAAG 
CTGAACCAAA AGTTTGATTG GTTTTCAGCT TACGAAGAGC CGGTGGGAAA ACTGAGGTCG 
ATTAAAGCCC AAGATAAAGT GTTGTATGAG ATCTTTAAAC CGGATCATAA GGCTGTCACT 
GTGATGCTGC CAGTTGATGC ATCGGTGAAC GACATCTTGA CTACATTAGT GGATCCTGAC 
AGAAACTATG TGCTTGTCAA GATGAATTCC TCAGGAGACA GAGTCCAGCT GAAGTTGGAG 
ACCACAGCAG TGTCTGCCTC TTTAGGAGTG AACGAAAAGC TTTTCCTCTG CACTTCCAGC 
CAAGTTGAAC AACTGACACC TGATAAAGAG CAGCTGGGGC CGGAGAAGAG CACCATGGAC 
ACTCTGGAGC AGATTTGCTC CAAGGACATG GCCAGTCAAC ACACCAGCTA CGACTGGGAA 
CTCTTCATGG CCATGCATGA GGTGGAGCTT GTCTACTATG TTTTCGGACG GGAAAAGTTC 
CCTGGATCCA CCACAGCAAA CCTCGAGCGG TTTGTTCGTC GCTTCAATGA GGTTCAGTAC 
TGGGTTGTGA CTGAACTGTG TCTCTGTGAA GACCTGGGCA AGAGAGCCAT ACTGCTGAAG 
AAGTTCATCA AGATGGCTGT TGTTTTGAAA GAGCAGAAGA ACCTCAACTC TTTTTTCGCA 
GTAATGTTTG GCCTCAGCAA CAGTGCAGTG CAGAGGCTCA ATAAAACATG GGAGAGACTT 
CCCAATAAAA CCAAAAGGAT CTACTGTGCA TACGAAAGAC TGATGGATCC GTCCCGTAAC 
CACAGGGCCT ACAGACTGAC TATTGCCAAA CTCAGCCCTC CATACATTCC CTTCATGCCA 
CTGCTTCTAA AAGATATGAC ATTTATTCAT GAGGGAAACA AGAACTACAC TGATAAACTG 
GTCAACTTTG AGAAAATGCG CATGATTGCC AGAACAGTGA AGACAGTTCG GGATTGTCGA 
AGCCAACCTT ACGTGCCTTC ATCTCCGCAG AAAGGCTTGA CGGAGAGAAT GTTCTTGGAT 
GCTCAAGCTA TCCGAATATC AACATATTCA GACCAGTCTC TGAACCTCCG CAGTGCCACC 
AGTATCAGAC AGTACATCCA GAACCTGAAA GTGATCGACA ATCAGAAGAA ACTAACTCAG 
CTCTCCAGAG CCTTAGAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297024.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295299.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa51778
Clone ID Related Accession (Same CDS sequence)	XM_009297024.3 , XM_009297024.2
Accession Version	XM_009297024.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGATT CAGACCAGTC TCTGAACCTC CGCAGTGCCA CCAGTATCAG ACAGTACATC 
CAGAACCTGA AAGTGATCGA CAATCAGAAG AAACTAACTC AGCTCTCCAG AGCCTTAGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295299.1
CDS	59..2644
Translation	MEEIHARMFSFFKQILSAKKKNRLESPAVMCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYDSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297024.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297024.3

ORF Insert Sequence:

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGATT CAGACCAGTC TCTGAACCTC CGCAGTGCCA CCAGTATCAG ACAGTACATC 
CAGAACCTGA AAGTGATCGA CAATCAGAAG AAACTAACTC AGCTCTCCAG AGCCTTAGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297024.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295299.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa51778
Clone ID Related Accession (Same CDS sequence)	XM_009297024.3 , XM_009297024.2
Accession Version	XM_009297024.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGATT CAGACCAGTC TCTGAACCTC CGCAGTGCCA CCAGTATCAG ACAGTACATC 
CAGAACCTGA AAGTGATCGA CAATCAGAAG AAACTAACTC AGCTCTCCAG AGCCTTAGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295299.1
CDS	63..2648
Translation	MEEIHARMFSFFKQILSAKKKNRLESPAVMCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYDSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297024.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297024.2

ORF Insert Sequence:

ATGGAGGAAA TACATGCCAG AATGTTTTCG TTTTTTAAAC AGATCTTGTC TGCTAAGAAG 
AAAAACAGAC TCGAGTCTCC AGCAGTCATG TGTTCGGCAG ACACACTCCT CCGCTCCACT 
CTGCGCTCGT CCCTTCAAAA GACGCCTCTG CCAGACGCTG TGGATACACA AGACACCATC 
AAACAGTTTT TATCAGATCG AATCCTGAAG GCTGCACGGG TAGTTTACAG CACTCTGATG 
GAGGGTAACC CAGGCCTGAT AAGAGACAGA AAACACCATC TTAAGACCTA CAGACAGTGC 
TGCAGCGGAA AGGAGCTTGT GGATTGGTTA ATGAAACTCA ATGATTGTTT CCAGTCCCGG 
AGTCAGGCAG TCGGAATGTG GCAGGTTTTG GTGGATGAGG GCATTTTGGG TCATGTAAAA 
CAGGAGTTAA ACTTCCATGA CAAAGACACA CAGTTTTACC GCTTTATGGA AGCAGAGTTT 
GACCTCAATC ACACAACTAA CGAAAAAGAC TCCAAAGACG ACGAACTACA AGAGAGTTTA 
TCGCTACTAA TCCAGATGGG CCCAGATGCT CTTTTGACGA TGATACTGCG TAAATGCCCA 
AGCCAGAGAA CCCCAGAAGA TCTGGAAGTT ATTTATGAAG AACTTCTGCA CGTCAAAGCT 
GTGGCCCACT TGTCTACATC TGTTCGTAAA GAGCTGGCAT CAGTGCTGGT ATTTGAAAGC 
CATGCCAAGG CGGGAACAGT CTTGTTCAGT CAGGGGGACA AAGGGACTTC CTGGTACATC 
ATCTGGAGAG GCTCTGTTAA TGTCATCACA CACGGCAAGG GCTTGGTGAC TACGCTTCAT 
GAAGGAGATG ATTTTGGCCA GTTGGCATTG GTGAATGACG CTCCTCGAGC CGCCACCATC 
ATTTTAAGAG AGGACAATTG CCACTTTCTG CGGGTGGACA AGCAGGATTT TATACGCATT 
CTTAAGGACG TCGAGGCAAA CACCGTCCGT CTGGAGGAGC ATGGTAAAGT CGTTCTGGTT 
CTTGAGAAGA GCTCGGCGCA GGATACACCA AGCTGCAACA AGTACACGGT AATGTCAGGA 
ACGCCGGAAA AGATCCTGGA GCACATGCTG GAGGCCATAA AACTGGAAAC CAACGGAGCT 
GACTTCATAG ATCCCTGTGT GACTGACTTT CTCCTAACGC ATCCCGTCTT CATGCCCTGC 
AGTCAACTGT GTGCTGCCCT CCAGCATCAT TACCAAGCGG AGCCGTCTGA AGGCTCAGAC 
CTGGAGAAGG CAGCGTACGC TCTCAACACC AAGCAGAAGG TGGTGAAGCT GGTGTGTCAC 
TGGGTGGCTC TGTTCGCTCT GCTGCTCAGG GACGATCCTG TGGCCTCTGA ATTCCTGGAG 
AAATTCAGGG AAGGAGTGAT GGCAGACTCA AGACTTTCTA GCATGCTTAG AGAACAGCTA 
AGGGACAGGA GGAAGACAAA AATAATGGAG AATGGATGTC ACACTCTGAC AAAGCTGAAC 
CAAAAGTTTG ATTGGTTTTC AGCTTACGAA GAGCCGGTGG GAAAACTGAG GTCGATTAAA 
GCCCAAGATA AAGTGTTGTA TGAGATCTTT AAACCGGATC ATAAGGCTGT CACTGTGATG 
CTGCCAGTTG ATGCATCGGT GAACGACATC TTGACTACAT TAGTGGATCC TGACAGAAAC 
TATGTGCTTG TCAAGATGAA TTCCTCAGGA GACAGAGTCC AGCTGAAGTT GGAGACCACA 
GCAGTGTCTG CCTCTTTAGG AGTGAACGAA AAGCTTTTCC TCTGCACTTC CAGCCAAGTT 
GAACAACTGA CACCTGATAA AGAGCAGCTG GGGCCGGAGA AGAGCACCAT GGACACTCTG 
GAGCAGATTT GCTCCAAGGA CATGGCCAGT CAACACACCA GCTACGACTG GGAACTCTTC 
ATGGCCATGC ATGAGGTGGA GCTTGTCTAC TATGTTTTCG GACGGGAAAA GTTCCCTGGA 
TCCACCACAG CAAACCTCGA GCGGTTTGTT CGTCGCTTCA ATGAGGTTCA GTACTGGGTT 
GTGACTGAAC TGTGTCTCTG TGAAGACCTG GGCAAGAGAG CCATACTGCT GAAGAAGTTC 
ATCAAGATGG CTGTTGTTTT GAAAGAGCAG AAGAACCTCA ACTCTTTTTT CGCAGTAATG 
TTTGGCCTCA GCAACAGTGC AGTGCAGAGG CTCAATAAAA CATGGGAGAG ACTTCCCAAT 
AAAACCAAAA GGATCTACTG TGCATACGAA AGACTGATGG ATCCGTCCCG TAACCACAGG 
GCCTACAGAC TGACTATTGC CAAACTCAGC CCTCCATACA TTCCCTTCAT GCCACTGCTT 
CTAAAAGATA TGACATTTAT TCATGAGGGA AACAAGAACT ACACTGATAA ACTGGTCAAC 
TTTGAGAAAA TGCGCATGAT TGCCAGAACA GTGAAGACAG TTCGGGATTG TCGAAGCCAA 
CCTTACGATT CAGACCAGTC TCTGAACCTC CGCAGTGCCA CCAGTATCAG ACAGTACATC 
CAGAACCTGA AAGTGATCGA CAATCAGAAG AAACTAACTC AGCTCTCCAG AGCCTTAGAG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297025.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295300.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa51779
Clone ID Related Accession (Same CDS sequence)	XM_009297025.2 , XM_009297025.3
Accession Version	XM_009297025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTCGG CAGACACACT CCTCCGCTCC ACTCTGCGCT CGTCCCTTCA AAAGACGCCT 
CTGCCAGACG CTGTGGATAC ACAAGACACC ATCAAACAGT TTTTATCAGA TCGAATCCTG 
AAGGCTGCAC GGGTAGTTTA CAGCACTCTG ATGGAGGGTA ACCCAGGCCT GATAAGAGAC 
AGAAAACACC ATCTTAAGAC CTACAGACAG TGCTGCAGCG GAAAGGAGCT TGTGGATTGG 
TTAATGAAAC TCAATGATTG TTTCCAGTCC CGGAGTCAGG CAGTCGGAAT GTGGCAGGTT 
TTGGTGGATG AGGGCATTTT GGGTCATGTA AAACAGGAGT TAAACTTCCA TGACAAAGAC 
ACACAGTTTT ACCGCTTTAT GGAAGCAGAG TTTGACCTCA ATCACACAAC TAACGAAAAA 
GACTCCAAAG ACGACGAACT ACAAGAGAGT TTATCGCTAC TAATCCAGAT GGGCCCAGAT 
GCTCTTTTGA CGATGATACT GCGTAAATGC CCAAGCCAGA GAACCCCAGA AGATCTGGAA 
GTTATTTATG AAGAACTTCT GCACGTCAAA GCTGTGGCCC ACTTGTCTAC ATCTGTTCGT 
AAAGAGCTGG CATCAGTGCT GGTATTTGAA AGCCATGCCA AGGCGGGAAC AGTCTTGTTC 
AGTCAGGGGG ACAAAGGGAC TTCCTGGTAC ATCATCTGGA GAGGCTCTGT TAATGTCATC 
ACACACGGCA AGGGCTTGGT GACTACGCTT CATGAAGGAG ATGATTTTGG CCAGTTGGCA 
TTGGTGAATG ACGCTCCTCG AGCCGCCACC ATCATTTTAA GAGAGGACAA TTGCCACTTT 
CTGCGGGTGG ACAAGCAGGA TTTTATACGC ATTCTTAAGG ACGTCGAGGC AAACACCGTC 
CGTCTGGAGG AGCATGGTAA AGTCGTTCTG GTTCTTGAGA AGAGCTCGGC GCAGGATACA 
CCAAGCTGCA ACAAGTACAC GGTAATGTCA GGAACGCCGG AAAAGATCCT GGAGCACATG 
CTGGAGGCCA TAAAACTGGA AACCAACGGA GCTGACTTCA TAGATCCCTG TGTGACTGAC 
TTTCTCCTAA CGCATCCCGT CTTCATGCCC TGCAGTCAAC TGTGTGCTGC CCTCCAGCAT 
CATTACCAAG CGGAGCCGTC TGAAGGCTCA GACCTGGAGA AGGCAGCGTA CGCTCTCAAC 
ACCAAGCAGA AGGTGGTGAA GCTGGTGTGT CACTGGGTGG CTCTGTTCGC TCTGCTGCTC 
AGGGACGATC CTGTGGCCTC TGAATTCCTG GAGAAATTCA GGGAAGGAGT GATGGCAGAC 
TCAAGACTTT CTAGCATGCT TAGAGAACAG CTAAGGGACA GGAGGAAGAC AAAAATAATG 
GAGAATGGAT GTCACACTCT GACAAAGCTG AACCAAAAGT TTGATTGGTT TTCAGCTTAC 
GAAGAGCCGG TGGGAAAACT GAGGTCGATT AAAGCCCAAG ATAAAGTGTT GTATGAGATC 
TTTAAACCGG ATCATAAGGC TGTCACTGTG ATGCTGCCAG TTGATGCATC GGTGAACGAC 
ATCTTGACTA CATTAGTGGA TCCTGACAGA AACTATGTGC TTGTCAAGAT GAATTCCTCA 
GGAGACAGAG TCCAGCTGAA GTTGGAGACC ACAGCAGTGT CTGCCTCTTT AGGAGTGAAC 
GAAAAGCTTT TCCTCTGCAC TTCCAGCCAA GTTGAACAAC TGACACCTGA TAAAGAGCAG 
CTGGGGCCGG AGAAGAGCAC CATGGACACT CTGGAGCAGA TTTGCTCCAA GGACATGGCC 
AGTCAACACA CCAGCTACGA CTGGGAACTC TTCATGGCCA TGCATGAGGT GGAGCTTGTC 
TACTATGTTT TCGGACGGGA AAAGTTCCCT GGATCCACCA CAGCAAACCT CGAGCGGTTT 
GTTCGTCGCT TCAATGAGGT TCAGTACTGG GTTGTGACTG AACTGTGTCT CTGTGAAGAC 
CTGGGCAAGA GAGCCATACT GCTGAAGAAG TTCATCAAGA TGGCTGTTGT TTTGAAAGAG 
CAGAAGAACC TCAACTCTTT TTTCGCAGTA ATGTTTGGCC TCAGCAACAG TGCAGTGCAG 
AGGCTCAATA AAACATGGGA GAGACTTCCC AATAAAACCA AAAGGATCTA CTGTGCATAC 
GAAAGACTGA TGGATCCGTC CCGTAACCAC AGGGCCTACA GACTGACTAT TGCCAAACTC 
AGCCCTCCAT ACATTCCCTT CATGCCACTG CTTCTAAAAG ATATGACATT TATTCATGAG 
GGAAACAAGA ACTACACTGA TAAACTGGTC AACTTTGAGA AAATGCGCAT GATTGCCAGA 
ACAGTGAAGA CAGTTCGGGA TTGTCGAAGC CAACCTTACG TGCCTTCATC TCCGCAGAAA 
GGCTTGACGG AGAGAATGTT CTTGGATGCT CAAGCTATCC GAATATCAAC ATATTCAGAC 
CAGTCTCTGA ACCTCCGCAG TGCCACCAGT ATCAGACAGT ACATCCAGAA CCTGAAAGTG 
ATCGACAATC AGAAGAAACT AACTCAGCTC TCCAGAGCCT TAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295300.1
CDS	182..2752
Translation	MCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297025.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297025.2

ORF Insert Sequence:

ATGTGTTCGG CAGACACACT CCTCCGCTCC ACTCTGCGCT CGTCCCTTCA AAAGACGCCT 
CTGCCAGACG CTGTGGATAC ACAAGACACC ATCAAACAGT TTTTATCAGA TCGAATCCTG 
AAGGCTGCAC GGGTAGTTTA CAGCACTCTG ATGGAGGGTA ACCCAGGCCT GATAAGAGAC 
AGAAAACACC ATCTTAAGAC CTACAGACAG TGCTGCAGCG GAAAGGAGCT TGTGGATTGG 
TTAATGAAAC TCAATGATTG TTTCCAGTCC CGGAGTCAGG CAGTCGGAAT GTGGCAGGTT 
TTGGTGGATG AGGGCATTTT GGGTCATGTA AAACAGGAGT TAAACTTCCA TGACAAAGAC 
ACACAGTTTT ACCGCTTTAT GGAAGCAGAG TTTGACCTCA ATCACACAAC TAACGAAAAA 
GACTCCAAAG ACGACGAACT ACAAGAGAGT TTATCGCTAC TAATCCAGAT GGGCCCAGAT 
GCTCTTTTGA CGATGATACT GCGTAAATGC CCAAGCCAGA GAACCCCAGA AGATCTGGAA 
GTTATTTATG AAGAACTTCT GCACGTCAAA GCTGTGGCCC ACTTGTCTAC ATCTGTTCGT 
AAAGAGCTGG CATCAGTGCT GGTATTTGAA AGCCATGCCA AGGCGGGAAC AGTCTTGTTC 
AGTCAGGGGG ACAAAGGGAC TTCCTGGTAC ATCATCTGGA GAGGCTCTGT TAATGTCATC 
ACACACGGCA AGGGCTTGGT GACTACGCTT CATGAAGGAG ATGATTTTGG CCAGTTGGCA 
TTGGTGAATG ACGCTCCTCG AGCCGCCACC ATCATTTTAA GAGAGGACAA TTGCCACTTT 
CTGCGGGTGG ACAAGCAGGA TTTTATACGC ATTCTTAAGG ACGTCGAGGC AAACACCGTC 
CGTCTGGAGG AGCATGGTAA AGTCGTTCTG GTTCTTGAGA AGAGCTCGGC GCAGGATACA 
CCAAGCTGCA ACAAGTACAC GGTAATGTCA GGAACGCCGG AAAAGATCCT GGAGCACATG 
CTGGAGGCCA TAAAACTGGA AACCAACGGA GCTGACTTCA TAGATCCCTG TGTGACTGAC 
TTTCTCCTAA CGCATCCCGT CTTCATGCCC TGCAGTCAAC TGTGTGCTGC CCTCCAGCAT 
CATTACCAAG CGGAGCCGTC TGAAGGCTCA GACCTGGAGA AGGCAGCGTA CGCTCTCAAC 
ACCAAGCAGA AGGTGGTGAA GCTGGTGTGT CACTGGGTGG CTCTGTTCGC TCTGCTGCTC 
AGGGACGATC CTGTGGCCTC TGAATTCCTG GAGAAATTCA GGGAAGGAGT GATGGCAGAC 
TCAAGACTTT CTAGCATGCT TAGAGAACAG CTAAGGGACA GGAGGAAGAC AAAAATAATG 
GAGAATGGAT GTCACACTCT GACAAAGCTG AACCAAAAGT TTGATTGGTT TTCAGCTTAC 
GAAGAGCCGG TGGGAAAACT GAGGTCGATT AAAGCCCAAG ATAAAGTGTT GTATGAGATC 
TTTAAACCGG ATCATAAGGC TGTCACTGTG ATGCTGCCAG TTGATGCATC GGTGAACGAC 
ATCTTGACTA CATTAGTGGA TCCTGACAGA AACTATGTGC TTGTCAAGAT GAATTCCTCA 
GGAGACAGAG TCCAGCTGAA GTTGGAGACC ACAGCAGTGT CTGCCTCTTT AGGAGTGAAC 
GAAAAGCTTT TCCTCTGCAC TTCCAGCCAA GTTGAACAAC TGACACCTGA TAAAGAGCAG 
CTGGGGCCGG AGAAGAGCAC CATGGACACT CTGGAGCAGA TTTGCTCCAA GGACATGGCC 
AGTCAACACA CCAGCTACGA CTGGGAACTC TTCATGGCCA TGCATGAGGT GGAGCTTGTC 
TACTATGTTT TCGGACGGGA AAAGTTCCCT GGATCCACCA CAGCAAACCT CGAGCGGTTT 
GTTCGTCGCT TCAATGAGGT TCAGTACTGG GTTGTGACTG AACTGTGTCT CTGTGAAGAC 
CTGGGCAAGA GAGCCATACT GCTGAAGAAG TTCATCAAGA TGGCTGTTGT TTTGAAAGAG 
CAGAAGAACC TCAACTCTTT TTTCGCAGTA ATGTTTGGCC TCAGCAACAG TGCAGTGCAG 
AGGCTCAATA AAACATGGGA GAGACTTCCC AATAAAACCA AAAGGATCTA CTGTGCATAC 
GAAAGACTGA TGGATCCGTC CCGTAACCAC AGGGCCTACA GACTGACTAT TGCCAAACTC 
AGCCCTCCAT ACATTCCCTT CATGCCACTG CTTCTAAAAG ATATGACATT TATTCATGAG 
GGAAACAAGA ACTACACTGA TAAACTGGTC AACTTTGAGA AAATGCGCAT GATTGCCAGA 
ACAGTGAAGA CAGTTCGGGA TTGTCGAAGC CAACCTTACG TGCCTTCATC TCCGCAGAAA 
GGCTTGACGG AGAGAATGTT CTTGGATGCT CAAGCTATCC GAATATCAAC ATATTCAGAC 
CAGTCTCTGA ACCTCCGCAG TGCCACCAGT ATCAGACAGT ACATCCAGAA CCTGAAAGTG 
ATCGACAATC AGAAGAAACT AACTCAGCTC TCCAGAGCCT TAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_009297025.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295300.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa51779
Clone ID Related Accession (Same CDS sequence)	XM_009297025.2 , XM_009297025.3
Accession Version	XM_009297025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTCGG CAGACACACT CCTCCGCTCC ACTCTGCGCT CGTCCCTTCA AAAGACGCCT 
CTGCCAGACG CTGTGGATAC ACAAGACACC ATCAAACAGT TTTTATCAGA TCGAATCCTG 
AAGGCTGCAC GGGTAGTTTA CAGCACTCTG ATGGAGGGTA ACCCAGGCCT GATAAGAGAC 
AGAAAACACC ATCTTAAGAC CTACAGACAG TGCTGCAGCG GAAAGGAGCT TGTGGATTGG 
TTAATGAAAC TCAATGATTG TTTCCAGTCC CGGAGTCAGG CAGTCGGAAT GTGGCAGGTT 
TTGGTGGATG AGGGCATTTT GGGTCATGTA AAACAGGAGT TAAACTTCCA TGACAAAGAC 
ACACAGTTTT ACCGCTTTAT GGAAGCAGAG TTTGACCTCA ATCACACAAC TAACGAAAAA 
GACTCCAAAG ACGACGAACT ACAAGAGAGT TTATCGCTAC TAATCCAGAT GGGCCCAGAT 
GCTCTTTTGA CGATGATACT GCGTAAATGC CCAAGCCAGA GAACCCCAGA AGATCTGGAA 
GTTATTTATG AAGAACTTCT GCACGTCAAA GCTGTGGCCC ACTTGTCTAC ATCTGTTCGT 
AAAGAGCTGG CATCAGTGCT GGTATTTGAA AGCCATGCCA AGGCGGGAAC AGTCTTGTTC 
AGTCAGGGGG ACAAAGGGAC TTCCTGGTAC ATCATCTGGA GAGGCTCTGT TAATGTCATC 
ACACACGGCA AGGGCTTGGT GACTACGCTT CATGAAGGAG ATGATTTTGG CCAGTTGGCA 
TTGGTGAATG ACGCTCCTCG AGCCGCCACC ATCATTTTAA GAGAGGACAA TTGCCACTTT 
CTGCGGGTGG ACAAGCAGGA TTTTATACGC ATTCTTAAGG ACGTCGAGGC AAACACCGTC 
CGTCTGGAGG AGCATGGTAA AGTCGTTCTG GTTCTTGAGA AGAGCTCGGC GCAGGATACA 
CCAAGCTGCA ACAAGTACAC GGTAATGTCA GGAACGCCGG AAAAGATCCT GGAGCACATG 
CTGGAGGCCA TAAAACTGGA AACCAACGGA GCTGACTTCA TAGATCCCTG TGTGACTGAC 
TTTCTCCTAA CGCATCCCGT CTTCATGCCC TGCAGTCAAC TGTGTGCTGC CCTCCAGCAT 
CATTACCAAG CGGAGCCGTC TGAAGGCTCA GACCTGGAGA AGGCAGCGTA CGCTCTCAAC 
ACCAAGCAGA AGGTGGTGAA GCTGGTGTGT CACTGGGTGG CTCTGTTCGC TCTGCTGCTC 
AGGGACGATC CTGTGGCCTC TGAATTCCTG GAGAAATTCA GGGAAGGAGT GATGGCAGAC 
TCAAGACTTT CTAGCATGCT TAGAGAACAG CTAAGGGACA GGAGGAAGAC AAAAATAATG 
GAGAATGGAT GTCACACTCT GACAAAGCTG AACCAAAAGT TTGATTGGTT TTCAGCTTAC 
GAAGAGCCGG TGGGAAAACT GAGGTCGATT AAAGCCCAAG ATAAAGTGTT GTATGAGATC 
TTTAAACCGG ATCATAAGGC TGTCACTGTG ATGCTGCCAG TTGATGCATC GGTGAACGAC 
ATCTTGACTA CATTAGTGGA TCCTGACAGA AACTATGTGC TTGTCAAGAT GAATTCCTCA 
GGAGACAGAG TCCAGCTGAA GTTGGAGACC ACAGCAGTGT CTGCCTCTTT AGGAGTGAAC 
GAAAAGCTTT TCCTCTGCAC TTCCAGCCAA GTTGAACAAC TGACACCTGA TAAAGAGCAG 
CTGGGGCCGG AGAAGAGCAC CATGGACACT CTGGAGCAGA TTTGCTCCAA GGACATGGCC 
AGTCAACACA CCAGCTACGA CTGGGAACTC TTCATGGCCA TGCATGAGGT GGAGCTTGTC 
TACTATGTTT TCGGACGGGA AAAGTTCCCT GGATCCACCA CAGCAAACCT CGAGCGGTTT 
GTTCGTCGCT TCAATGAGGT TCAGTACTGG GTTGTGACTG AACTGTGTCT CTGTGAAGAC 
CTGGGCAAGA GAGCCATACT GCTGAAGAAG TTCATCAAGA TGGCTGTTGT TTTGAAAGAG 
CAGAAGAACC TCAACTCTTT TTTCGCAGTA ATGTTTGGCC TCAGCAACAG TGCAGTGCAG 
AGGCTCAATA AAACATGGGA GAGACTTCCC AATAAAACCA AAAGGATCTA CTGTGCATAC 
GAAAGACTGA TGGATCCGTC CCGTAACCAC AGGGCCTACA GACTGACTAT TGCCAAACTC 
AGCCCTCCAT ACATTCCCTT CATGCCACTG CTTCTAAAAG ATATGACATT TATTCATGAG 
GGAAACAAGA ACTACACTGA TAAACTGGTC AACTTTGAGA AAATGCGCAT GATTGCCAGA 
ACAGTGAAGA CAGTTCGGGA TTGTCGAAGC CAACCTTACG TGCCTTCATC TCCGCAGAAA 
GGCTTGACGG AGAGAATGTT CTTGGATGCT CAAGCTATCC GAATATCAAC ATATTCAGAC 
CAGTCTCTGA ACCTCCGCAG TGCCACCAGT ATCAGACAGT ACATCCAGAA CCTGAAAGTG 
ATCGACAATC AGAAGAAACT AACTCAGCTC TCCAGAGCCT TAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295300.1
CDS	248..2818
Translation	MCSADTLLRSTLRSSLQKTPLPDAVDTQDTIKQFLSDRILKAARVVYSTLMEGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNFHDKDTQFYRFMEAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILRKCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIWRGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEANTVRLEEHGKVVLVLEKSSAQDTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFLLTHPVFMPCSQLCAALQHHYQAEPSEGSDLEKAAYALNTKQKVVKLVCHWVALFALLLRDDPVASEFLEKFREGVMADSRLSSMLREQLRDRRKTKIMENGCHTLTKLNQKFDWFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVDPDRNYVLVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKDMASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGKRAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSRNHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQPYVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALER

Target ORF information:

RefSeq Version	XM_009297025.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 3 (rapgef3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297025.3

ORF Insert Sequence:

ATGTGTTCGG CAGACACACT CCTCCGCTCC ACTCTGCGCT CGTCCCTTCA AAAGACGCCT 
CTGCCAGACG CTGTGGATAC ACAAGACACC ATCAAACAGT TTTTATCAGA TCGAATCCTG 
AAGGCTGCAC GGGTAGTTTA CAGCACTCTG ATGGAGGGTA ACCCAGGCCT GATAAGAGAC 
AGAAAACACC ATCTTAAGAC CTACAGACAG TGCTGCAGCG GAAAGGAGCT TGTGGATTGG 
TTAATGAAAC TCAATGATTG TTTCCAGTCC CGGAGTCAGG CAGTCGGAAT GTGGCAGGTT 
TTGGTGGATG AGGGCATTTT GGGTCATGTA AAACAGGAGT TAAACTTCCA TGACAAAGAC 
ACACAGTTTT ACCGCTTTAT GGAAGCAGAG TTTGACCTCA ATCACACAAC TAACGAAAAA 
GACTCCAAAG ACGACGAACT ACAAGAGAGT TTATCGCTAC TAATCCAGAT GGGCCCAGAT 
GCTCTTTTGA CGATGATACT GCGTAAATGC CCAAGCCAGA GAACCCCAGA AGATCTGGAA 
GTTATTTATG AAGAACTTCT GCACGTCAAA GCTGTGGCCC ACTTGTCTAC ATCTGTTCGT 
AAAGAGCTGG CATCAGTGCT GGTATTTGAA AGCCATGCCA AGGCGGGAAC AGTCTTGTTC 
AGTCAGGGGG ACAAAGGGAC TTCCTGGTAC ATCATCTGGA GAGGCTCTGT TAATGTCATC 
ACACACGGCA AGGGCTTGGT GACTACGCTT CATGAAGGAG ATGATTTTGG CCAGTTGGCA 
TTGGTGAATG ACGCTCCTCG AGCCGCCACC ATCATTTTAA GAGAGGACAA TTGCCACTTT 
CTGCGGGTGG ACAAGCAGGA TTTTATACGC ATTCTTAAGG ACGTCGAGGC AAACACCGTC 
CGTCTGGAGG AGCATGGTAA AGTCGTTCTG GTTCTTGAGA AGAGCTCGGC GCAGGATACA 
CCAAGCTGCA ACAAGTACAC GGTAATGTCA GGAACGCCGG AAAAGATCCT GGAGCACATG 
CTGGAGGCCA TAAAACTGGA AACCAACGGA GCTGACTTCA TAGATCCCTG TGTGACTGAC 
TTTCTCCTAA CGCATCCCGT CTTCATGCCC TGCAGTCAAC TGTGTGCTGC CCTCCAGCAT 
CATTACCAAG CGGAGCCGTC TGAAGGCTCA GACCTGGAGA AGGCAGCGTA CGCTCTCAAC 
ACCAAGCAGA AGGTGGTGAA GCTGGTGTGT CACTGGGTGG CTCTGTTCGC TCTGCTGCTC 
AGGGACGATC CTGTGGCCTC TGAATTCCTG GAGAAATTCA GGGAAGGAGT GATGGCAGAC 
TCAAGACTTT CTAGCATGCT TAGAGAACAG CTAAGGGACA GGAGGAAGAC AAAAATAATG 
GAGAATGGAT GTCACACTCT GACAAAGCTG AACCAAAAGT TTGATTGGTT TTCAGCTTAC 
GAAGAGCCGG TGGGAAAACT GAGGTCGATT AAAGCCCAAG ATAAAGTGTT GTATGAGATC 
TTTAAACCGG ATCATAAGGC TGTCACTGTG ATGCTGCCAG TTGATGCATC GGTGAACGAC 
ATCTTGACTA CATTAGTGGA TCCTGACAGA AACTATGTGC TTGTCAAGAT GAATTCCTCA 
GGAGACAGAG TCCAGCTGAA GTTGGAGACC ACAGCAGTGT CTGCCTCTTT AGGAGTGAAC 
GAAAAGCTTT TCCTCTGCAC TTCCAGCCAA GTTGAACAAC TGACACCTGA TAAAGAGCAG 
CTGGGGCCGG AGAAGAGCAC CATGGACACT CTGGAGCAGA TTTGCTCCAA GGACATGGCC 
AGTCAACACA CCAGCTACGA CTGGGAACTC TTCATGGCCA TGCATGAGGT GGAGCTTGTC 
TACTATGTTT TCGGACGGGA AAAGTTCCCT GGATCCACCA CAGCAAACCT CGAGCGGTTT 
GTTCGTCGCT TCAATGAGGT TCAGTACTGG GTTGTGACTG AACTGTGTCT CTGTGAAGAC 
CTGGGCAAGA GAGCCATACT GCTGAAGAAG TTCATCAAGA TGGCTGTTGT TTTGAAAGAG 
CAGAAGAACC TCAACTCTTT TTTCGCAGTA ATGTTTGGCC TCAGCAACAG TGCAGTGCAG 
AGGCTCAATA AAACATGGGA GAGACTTCCC AATAAAACCA AAAGGATCTA CTGTGCATAC 
GAAAGACTGA TGGATCCGTC CCGTAACCAC AGGGCCTACA GACTGACTAT TGCCAAACTC 
AGCCCTCCAT ACATTCCCTT CATGCCACTG CTTCTAAAAG ATATGACATT TATTCATGAG 
GGAAACAAGA ACTACACTGA TAAACTGGTC AACTTTGAGA AAATGCGCAT GATTGCCAGA 
ACAGTGAAGA CAGTTCGGGA TTGTCGAAGC CAACCTTACG TGCCTTCATC TCCGCAGAAA 
GGCTTGACGG AGAGAATGTT CTTGGATGCT CAAGCTATCC GAATATCAAC ATATTCAGAC 
CAGTCTCTGA ACCTCCGCAG TGCCACCAGT ATCAGACAGT ACATCCAGAA CCTGAAAGTG 
ATCGACAATC AGAAGAAACT AACTCAGCTC TCCAGAGCCT TAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rapgef3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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