ssh1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ssh1a
Entrez Gene ID	101885659
Full Name	slingshot protein phosphatase 1a
Synonyms	si:ch211-108c6.1,ssh1,zC108C6.1
General protein information	Preferred Names slingshot protein phosphatase 1a Names protein phosphatase Slingshot homolog 1 slingshot homolog 1 slingshot homolog 1a slingshot protein phosphatase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301385.3	XP_009299660.1	protein phosphatase Slingshot homolog 1 isoform X2
XM_005165198.4	XP_005165255.1	protein phosphatase Slingshot homolog 1 isoform X3
XM_009301385.2	XP_009299660.1	protein phosphatase Slingshot homolog 1 isoform X1
XM_005165198.3	XP_005165255.1	protein phosphatase Slingshot homolog 1 isoform X2
XM_009301386.2	XP_009299661.1	protein phosphatase Slingshot homolog 1 isoform X3
XM_009301386.3	XP_009299661.1	protein phosphatase Slingshot homolog 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SSH1	XP_522524.3
	SSH1	XP_001096246.2
Mus musculus (house mouse)	Ssh1	NP_932777.2
Rattus norvegicus (Norway rat)	Ssh1	XP_006249574.1
Danio rerio (zebrafish)	ssh1b	XP_695867.2
Bos taurus (cattle)	SSH1	XP_002694647.2
Xenopus tropicalis (tropical clawed frog)	ssh1	XP_004910677.1
Homo sapiens (human)	SSH1	NP_061857.3
Gallus gallus (chicken)	SSH1	XP_004945673.1
Danio rerio (zebrafish)	LOC101885659	XP_005165255.1
Canis lupus familiaris (dog)	SSH1	XP_851712.1

Gene Ontology

The following ssh1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssh1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10322	XM_005165198.4 Latest version!	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa51660	XM_009301385.3 Latest version!	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa51661	XM_009301386.3 Latest version!	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa51660	XM_009301385.2	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10322	XM_005165198.3	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa51661	XM_009301386.2	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ssh1a ( XM_005165198.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165255.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10322
Clone ID Related Accession (Same CDS sequence)	XM_005165198.4 , XM_005165198.3
Accession Version	XM_005165198.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165198.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGACTTTGGA AGTGACGATG AGCGCCGGCT AAATCAGAGT 
CTGAGCGAGA GTTTCTTCAT GGTGAAGGGA GCCGCTCTGT TTCTGCAGCA GGGCAGTTGT 
CCACAGGCTC AGAGAGCTCA TCCTCACCAC AAACATGCAG GTGACTTGCC TCAGCATCTT 
CAGGTGATGA TCAACATTCT TCGGTCTGAG GATCGCATCA AACTGGCGGT GCGTTTGGAG 
AGCGCGTGGT CAGACCGTGT GAGGTACATG GTGGTGGTTT ACACAAATGG CCGACAAGAC 
ACTGAAGAAA ACATCTTATT GGGCATGGAC TTCAGCAACA AAGACAGTAA GAATTGCTCT 
GTCGGGATGG TGCTGCCGCT GTGGAGTGAC ACCAAGATTC ATTTAGACGG TGACGGGGGT 
TTCAGTGTGA CGACCGCCGG GCGTACGCAC ATCTTCAAAC CGGTGTCAGT CCAGGCCATG 
TGGTCAGCTT TGCAAATTTT ACACAAGGCC TGTGAGGTGT CACGGCGGTA CAACTATTTC 
CCTGGTGGGA TGGCACTGAC GTGGATGGGG TACTATGAGA GCTGCATCAG CTCTGAACAG 
AGCTGCATCA ATGAGTGGAA CGCCATGCAG GACCTGGAAA CACTGAGACC AGACTCGCCT 
GTAGTCTTTG TCGACAAGCC AACAGAGAGG GAGAGAACAG AATGTCTAAT CAAAGCCAAA 
CTCAGGAGTA TCATGATGTG CCAGGACCTT GAAAATGTCA CGTCCAAAGA GATTCGTAAT 
GAGTTGGAGC AGCAGATGAA CTGTAACCTG AAAGAGTACA AAGAGTTCAT AGACAATGAG 
ATGCTACTTA TTCTTGGCCA GATGGATAAA GCCACTCTCA TCTTTGACCA CCTTTATCTG 
GGTTCTGAAT GGAACGCTTC CAATCTAGAG GAACTTCAGG AAAGTGGGGT GGGCCACATC 
TTAAATGTCA CAAGGGAAAT AGATAACTTT TTCCCAGGAA CATTCTGCTA CCACAATGTC 
AGAGTGTATG ATGATGAGGC CACAGACTTA CTGGCTCACT GGAATGATAC CTACAACTTC 
ATTGTCAAAG CAAAAAAGAA TCATTCCAAA TGTTTGGTTC ACTGTAAGAT GGGCGTAAGC 
CGCTCTGCTT CCACGGTGAT CGCATATGCC ATGAAAGAGT ACGGCTGGAC TCTGGAGAAA 
GCATATAACT TTGTTAAACA GAAGCGCAGT ATCACTCGTC CCAATGCCAG CTTCATGAGA 
CAGCTGGCAG AGTATGAAGG CATACTGGAT GCCAGTAAGC AGCGTCATAA CCGTCTGTGG 
CGCCCTGATT CGGATTGCGA TCACCCCGAG TGCCAGCAAG CTGCTGGTCC TTGCTATCTG 
AATGCAGAGA CTGGAGATCA CATCACTCCT GACTCCACAA CCCCCAGGGT CACTGTCTGC 
TGTGAGCCTA CAGCCCACTC CTCTCCAATT AGTCTAGATC TGGATCCTGG CCATCCACAA 
AATTACCACT ACTACTTCCG CCGCTTGTCA GACTCGGCTC TAGAGAGCGA ACCTTCCACC 
CCTGTCCAAG CACAGCCTTT GGTGGATGTA GATCGTGTCT ATATTGAGGT TGCCGATGTG 
GAGCAAGATG CACTTCTTGA GGATGAAACC TTCGAGGCAC ATGAGGGCCC TCCTGTTTCC 
CACTCAGGAG AGGGTATTGG CGCTCAAACA TGTTCTCGAG GACCGGAGCC ACTAGAAGAA 
CTACGTCTGC GGTTGGAGTT CAGCACAGTG GAAGAAGAGG ATGAAGAAGA GGCTCAGAAA 
GAGCAAGCTG AGATGGAGGC GTTAACAGAG GAAGCAAAGA GAGTAGATGG GAAGGATAGT 
GAGGAGCTGT TGAATGAAAA GGGGATCGAT CTGACCAGTG TGAACCATTT GTGCAATGAG 
AACAACAACA ACAATCACCT CAAAATGCAA CAAATCCTCA CTGAAAGTGC CCAAGTAAAG 
ACAAGGCTTG CTCCTGGTTC ATCGCTTGCT TCCAAGCCCA TCCAAAGTGC TCTTTCGGAG 
GCTAACAGAC GAGGCCTTGC CCCTTTACCC CTCAACAGTG CGCCCTGTAT CTCCATTCAG 
ATGCTATCCT GTGCCAACTT GTCCATGACA GCCAAGTGTG GCAGAACTGT CTCAGATCCG 
TGCCTTGATG CCAATGCAAT GTCCTTAGGG AATAACGATA TGCCAGAAAG CAAATCTCTT 
GACCAGGAAA CTGAAACCCA GACAACTCTG CCTGAGGCCA AAGGAGAAGG AGATTTCAGG 
CAGGAAGAGA CTGATCTACA GGGTTCCTCT GCTCTTCCCC GGAATGAGAG AGAGCTGAAA 
GGTTTAGAGA AGGCAGAGGC GTCTTGTTCT CCTCAACAAC AAGAGGCTCT GATTCAGCTC 
CAACGCTCCG GTTTAGTTCG ACGTCGAAAA GAACGATTGG AAAAACTCTC CGGTCTCTTC 
CAGGAGCGTG CACGCGCAAA TGAACAACGG GAAGCTGGTT CTAGTAAAAC AGAAGATGCA 
ATGAACAGTT CCAGTGATCC AGCTGACCCC TTTCACAGGT CTTTTGGAAC ACGGAATGAT 
TTTGGAACTG AATCGGCAGT GCCACTGACC AATGAGGCTT TGTTGGCAGT CCTCGGATCA 
GGAGGATTGA CTCCAGTCTC ATCTCCACAC GGCTCTACGC TCACCCGCAG CTCCAGCAGT 
GACAGTCTTC GCAGTGTCCG AGGCAAACCA GGCCTTGTGC GTCAGCGTAC ACAAGAAATT 
GAAGCACGAA TGCGATTAGC GGGACTGACT GTTCCTTCAC GACTCAAGCG CTCTAACTCT 
CTCGCCAAAC TAGGCAGTCT CACCTTTTCA TCTGAGGACT TGTGCTCGGC CTGCTCCTCT 
GACGCAGGGA CCCTTCTCAT GCTCTCATTG TCCCCAGAAC CCGAGCACTC GCTCAGCCCA 
GAATGGGCAA AACCAGACAA CATGGAGGTC TTCAGCCAAG GCGAAGCAGG ATGTGAAGAG 
TCCAAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165255.1
CDS	1612..4683
Translation	MALVTLQRSPTPSAASTSSTATTNAGEDFGSDDERRLNQSLSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_005165198.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165198.4

ORF Insert Sequence:

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGACTTTGGA AGTGACGATG AGCGCCGGCT AAATCAGAGT 
CTGAGCGAGA GTTTCTTCAT GGTGAAGGGA GCCGCTCTGT TTCTGCAGCA GGGCAGTTGT 
CCACAGGCTC AGAGAGCTCA TCCTCACCAC AAACATGCAG GTGACTTGCC TCAGCATCTT 
CAGGTGATGA TCAACATTCT TCGGTCTGAG GATCGCATCA AACTGGCGGT GCGTTTGGAG 
AGCGCGTGGT CAGACCGTGT GAGGTACATG GTGGTGGTTT ACACAAATGG CCGACAAGAC 
ACTGAAGAAA ACATCTTATT GGGCATGGAC TTCAGCAACA AAGACAGTAA GAATTGCTCT 
GTCGGGATGG TGCTGCCGCT GTGGAGTGAC ACCAAGATTC ATTTAGACGG TGACGGGGGT 
TTCAGTGTGA CGACCGCCGG GCGTACGCAC ATCTTCAAAC CGGTGTCAGT CCAGGCCATG 
TGGTCAGCTT TGCAAATTTT ACACAAGGCC TGTGAGGTGT CACGGCGGTA CAACTATTTC 
CCTGGTGGGA TGGCACTGAC GTGGATGGGG TACTATGAGA GCTGCATCAG CTCTGAACAG 
AGCTGCATCA ATGAGTGGAA CGCCATGCAG GACCTGGAAA CACTGAGACC AGACTCGCCT 
GTAGTCTTTG TCGACAAGCC AACAGAGAGG GAGAGAACAG AATGTCTAAT CAAAGCCAAA 
CTCAGGAGTA TCATGATGTG CCAGGACCTT GAAAATGTCA CGTCCAAAGA GATTCGTAAT 
GAGTTGGAGC AGCAGATGAA CTGTAACCTG AAAGAGTACA AAGAGTTCAT AGACAATGAG 
ATGCTACTTA TTCTTGGCCA GATGGATAAA GCCACTCTCA TCTTTGACCA CCTTTATCTG 
GGTTCTGAAT GGAACGCTTC CAATCTAGAG GAACTTCAGG AAAGTGGGGT GGGCCACATC 
TTAAATGTCA CAAGGGAAAT AGATAACTTT TTCCCAGGAA CATTCTGCTA CCACAATGTC 
AGAGTGTATG ATGATGAGGC CACAGACTTA CTGGCTCACT GGAATGATAC CTACAACTTC 
ATTGTCAAAG CAAAAAAGAA TCATTCCAAA TGTTTGGTTC ACTGTAAGAT GGGCGTAAGC 
CGCTCTGCTT CCACGGTGAT CGCATATGCC ATGAAAGAGT ACGGCTGGAC TCTGGAGAAA 
GCATATAACT TTGTTAAACA GAAGCGCAGT ATCACTCGTC CCAATGCCAG CTTCATGAGA 
CAGCTGGCAG AGTATGAAGG CATACTGGAT GCCAGTAAGC AGCGTCATAA CCGTCTGTGG 
CGCCCTGATT CGGATTGCGA TCACCCCGAG TGCCAGCAAG CTGCTGGTCC TTGCTATCTG 
AATGCAGAGA CTGGAGATCA CATCACTCCT GACTCCACAA CCCCCAGGGT CACTGTCTGC 
TGTGAGCCTA CAGCCCACTC CTCTCCAATT AGTCTAGATC TGGATCCTGG CCATCCACAA 
AATTACCACT ACTACTTCCG CCGCTTGTCA GACTCGGCTC TAGAGAGCGA ACCTTCCACC 
CCTGTCCAAG CACAGCCTTT GGTGGATGTA GATCGTGTCT ATATTGAGGT TGCCGATGTG 
GAGCAAGATG CACTTCTTGA GGATGAAACC TTCGAGGCAC ATGAGGGCCC TCCTGTTTCC 
CACTCAGGAG AGGGTATTGG CGCTCAAACA TGTTCTCGAG GACCGGAGCC ACTAGAAGAA 
CTACGTCTGC GGTTGGAGTT CAGCACAGTG GAAGAAGAGG ATGAAGAAGA GGCTCAGAAA 
GAGCAAGCTG AGATGGAGGC GTTAACAGAG GAAGCAAAGA GAGTAGATGG GAAGGATAGT 
GAGGAGCTGT TGAATGAAAA GGGGATCGAT CTGACCAGTG TGAACCATTT GTGCAATGAG 
AACAACAACA ACAATCACCT CAAAATGCAA CAAATCCTCA CTGAAAGTGC CCAAGTAAAG 
ACAAGGCTTG CTCCTGGTTC ATCGCTTGCT TCCAAGCCCA TCCAAAGTGC TCTTTCGGAG 
GCTAACAGAC GAGGCCTTGC CCCTTTACCC CTCAACAGTG CGCCCTGTAT CTCCATTCAG 
ATGCTATCCT GTGCCAACTT GTCCATGACA GCCAAGTGTG GCAGAACTGT CTCAGATCCG 
TGCCTTGATG CCAATGCAAT GTCCTTAGGG AATAACGATA TGCCAGAAAG CAAATCTCTT 
GACCAGGAAA CTGAAACCCA GACAACTCTG CCTGAGGCCA AAGGAGAAGG AGATTTCAGG 
CAGGAAGAGA CTGATCTACA GGGTTCCTCT GCTCTTCCCC GGAATGAGAG AGAGCTGAAA 
GGTTTAGAGA AGGCAGAGGC GTCTTGTTCT CCTCAACAAC AAGAGGCTCT GATTCAGCTC 
CAACGCTCCG GTTTAGTTCG ACGTCGAAAA GAACGATTGG AAAAACTCTC CGGTCTCTTC 
CAGGAGCGTG CACGCGCAAA TGAACAACGG GAAGCTGGTT CTAGTAAAAC AGAAGATGCA 
ATGAACAGTT CCAGTGATCC AGCTGACCCC TTTCACAGGT CTTTTGGAAC ACGGAATGAT 
TTTGGAACTG AATCGGCAGT GCCACTGACC AATGAGGCTT TGTTGGCAGT CCTCGGATCA 
GGAGGATTGA CTCCAGTCTC ATCTCCACAC GGCTCTACGC TCACCCGCAG CTCCAGCAGT 
GACAGTCTTC GCAGTGTCCG AGGCAAACCA GGCCTTGTGC GTCAGCGTAC ACAAGAAATT 
GAAGCACGAA TGCGATTAGC GGGACTGACT GTTCCTTCAC GACTCAAGCG CTCTAACTCT 
CTCGCCAAAC TAGGCAGTCT CACCTTTTCA TCTGAGGACT TGTGCTCGGC CTGCTCCTCT 
GACGCAGGGA CCCTTCTCAT GCTCTCATTG TCCCCAGAAC CCGAGCACTC GCTCAGCCCA 
GAATGGGCAA AACCAGACAA CATGGAGGTC TTCAGCCAAG GCGAAGCAGG ATGTGAAGAG 
TCCAAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh1a ( XM_005165198.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165255.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10322
Clone ID Related Accession (Same CDS sequence)	XM_005165198.4 , XM_005165198.3
Accession Version	XM_005165198.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGACTTTGGA AGTGACGATG AGCGCCGGCT AAATCAGAGT 
CTGAGCGAGA GTTTCTTCAT GGTGAAGGGA GCCGCTCTGT TTCTGCAGCA GGGCAGTTGT 
CCACAGGCTC AGAGAGCTCA TCCTCACCAC AAACATGCAG GTGACTTGCC TCAGCATCTT 
CAGGTGATGA TCAACATTCT TCGGTCTGAG GATCGCATCA AACTGGCGGT GCGTTTGGAG 
AGCGCGTGGT CAGACCGTGT GAGGTACATG GTGGTGGTTT ACACAAATGG CCGACAAGAC 
ACTGAAGAAA ACATCTTATT GGGCATGGAC TTCAGCAACA AAGACAGTAA GAATTGCTCT 
GTCGGGATGG TGCTGCCGCT GTGGAGTGAC ACCAAGATTC ATTTAGACGG TGACGGGGGT 
TTCAGTGTGA CGACCGCCGG GCGTACGCAC ATCTTCAAAC CGGTGTCAGT CCAGGCCATG 
TGGTCAGCTT TGCAAATTTT ACACAAGGCC TGTGAGGTGT CACGGCGGTA CAACTATTTC 
CCTGGTGGGA TGGCACTGAC GTGGATGGGG TACTATGAGA GCTGCATCAG CTCTGAACAG 
AGCTGCATCA ATGAGTGGAA CGCCATGCAG GACCTGGAAA CACTGAGACC AGACTCGCCT 
GTAGTCTTTG TCGACAAGCC AACAGAGAGG GAGAGAACAG AATGTCTAAT CAAAGCCAAA 
CTCAGGAGTA TCATGATGTG CCAGGACCTT GAAAATGTCA CGTCCAAAGA GATTCGTAAT 
GAGTTGGAGC AGCAGATGAA CTGTAACCTG AAAGAGTACA AAGAGTTCAT AGACAATGAG 
ATGCTACTTA TTCTTGGCCA GATGGATAAA GCCACTCTCA TCTTTGACCA CCTTTATCTG 
GGTTCTGAAT GGAACGCTTC CAATCTAGAG GAACTTCAGG AAAGTGGGGT GGGCCACATC 
TTAAATGTCA CAAGGGAAAT AGATAACTTT TTCCCAGGAA CATTCTGCTA CCACAATGTC 
AGAGTGTATG ATGATGAGGC CACAGACTTA CTGGCTCACT GGAATGATAC CTACAACTTC 
ATTGTCAAAG CAAAAAAGAA TCATTCCAAA TGTTTGGTTC ACTGTAAGAT GGGCGTAAGC 
CGCTCTGCTT CCACGGTGAT CGCATATGCC ATGAAAGAGT ACGGCTGGAC TCTGGAGAAA 
GCATATAACT TTGTTAAACA GAAGCGCAGT ATCACTCGTC CCAATGCCAG CTTCATGAGA 
CAGCTGGCAG AGTATGAAGG CATACTGGAT GCCAGTAAGC AGCGTCATAA CCGTCTGTGG 
CGCCCTGATT CGGATTGCGA TCACCCCGAG TGCCAGCAAG CTGCTGGTCC TTGCTATCTG 
AATGCAGAGA CTGGAGATCA CATCACTCCT GACTCCACAA CCCCCAGGGT CACTGTCTGC 
TGTGAGCCTA CAGCCCACTC CTCTCCAATT AGTCTAGATC TGGATCCTGG CCATCCACAA 
AATTACCACT ACTACTTCCG CCGCTTGTCA GACTCGGCTC TAGAGAGCGA ACCTTCCACC 
CCTGTCCAAG CACAGCCTTT GGTGGATGTA GATCGTGTCT ATATTGAGGT TGCCGATGTG 
GAGCAAGATG CACTTCTTGA GGATGAAACC TTCGAGGCAC ATGAGGGCCC TCCTGTTTCC 
CACTCAGGAG AGGGTATTGG CGCTCAAACA TGTTCTCGAG GACCGGAGCC ACTAGAAGAA 
CTACGTCTGC GGTTGGAGTT CAGCACAGTG GAAGAAGAGG ATGAAGAAGA GGCTCAGAAA 
GAGCAAGCTG AGATGGAGGC GTTAACAGAG GAAGCAAAGA GAGTAGATGG GAAGGATAGT 
GAGGAGCTGT TGAATGAAAA GGGGATCGAT CTGACCAGTG TGAACCATTT GTGCAATGAG 
AACAACAACA ACAATCACCT CAAAATGCAA CAAATCCTCA CTGAAAGTGC CCAAGTAAAG 
ACAAGGCTTG CTCCTGGTTC ATCGCTTGCT TCCAAGCCCA TCCAAAGTGC TCTTTCGGAG 
GCTAACAGAC GAGGCCTTGC CCCTTTACCC CTCAACAGTG CGCCCTGTAT CTCCATTCAG 
ATGCTATCCT GTGCCAACTT GTCCATGACA GCCAAGTGTG GCAGAACTGT CTCAGATCCG 
TGCCTTGATG CCAATGCAAT GTCCTTAGGG AATAACGATA TGCCAGAAAG CAAATCTCTT 
GACCAGGAAA CTGAAACCCA GACAACTCTG CCTGAGGCCA AAGGAGAAGG AGATTTCAGG 
CAGGAAGAGA CTGATCTACA GGGTTCCTCT GCTCTTCCCC GGAATGAGAG AGAGCTGAAA 
GGTTTAGAGA AGGCAGAGGC GTCTTGTTCT CCTCAACAAC AAGAGGCTCT GATTCAGCTC 
CAACGCTCCG GTTTAGTTCG ACGTCGAAAA GAACGATTGG AAAAACTCTC CGGTCTCTTC 
CAGGAGCGTG CACGCGCAAA TGAACAACGG GAAGCTGGTT CTAGTAAAAC AGAAGATGCA 
ATGAACAGTT CCAGTGATCC AGCTGACCCC TTTCACAGGT CTTTTGGAAC ACGGAATGAT 
TTTGGAACTG AATCGGCAGT GCCACTGACC AATGAGGCTT TGTTGGCAGT CCTCGGATCA 
GGAGGATTGA CTCCAGTCTC ATCTCCACAC GGCTCTACGC TCACCCGCAG CTCCAGCAGT 
GACAGTCTTC GCAGTGTCCG AGGCAAACCA GGCCTTGTGC GTCAGCGTAC ACAAGAAATT 
GAAGCACGAA TGCGATTAGC GGGACTGACT GTTCCTTCAC GACTCAAGCG CTCTAACTCT 
CTCGCCAAAC TAGGCAGTCT CACCTTTTCA TCTGAGGACT TGTGCTCGGC CTGCTCCTCT 
GACGCAGGGA CCCTTCTCAT GCTCTCATTG TCCCCAGAAC CCGAGCACTC GCTCAGCCCA 
GAATGGGCAA AACCAGACAA CATGGAGGTC TTCAGCCAAG GCGAAGCAGG ATGTGAAGAG 
TCCAAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165255.1
CDS	1602..4673
Translation	MALVTLQRSPTPSAASTSSTATTNAGEDFGSDDERRLNQSLSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_005165198.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165198.3

ORF Insert Sequence:

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGACTTTGGA AGTGACGATG AGCGCCGGCT AAATCAGAGT 
CTGAGCGAGA GTTTCTTCAT GGTGAAGGGA GCCGCTCTGT TTCTGCAGCA GGGCAGTTGT 
CCACAGGCTC AGAGAGCTCA TCCTCACCAC AAACATGCAG GTGACTTGCC TCAGCATCTT 
CAGGTGATGA TCAACATTCT TCGGTCTGAG GATCGCATCA AACTGGCGGT GCGTTTGGAG 
AGCGCGTGGT CAGACCGTGT GAGGTACATG GTGGTGGTTT ACACAAATGG CCGACAAGAC 
ACTGAAGAAA ACATCTTATT GGGCATGGAC TTCAGCAACA AAGACAGTAA GAATTGCTCT 
GTCGGGATGG TGCTGCCGCT GTGGAGTGAC ACCAAGATTC ATTTAGACGG TGACGGGGGT 
TTCAGTGTGA CGACCGCCGG GCGTACGCAC ATCTTCAAAC CGGTGTCAGT CCAGGCCATG 
TGGTCAGCTT TGCAAATTTT ACACAAGGCC TGTGAGGTGT CACGGCGGTA CAACTATTTC 
CCTGGTGGGA TGGCACTGAC GTGGATGGGG TACTATGAGA GCTGCATCAG CTCTGAACAG 
AGCTGCATCA ATGAGTGGAA CGCCATGCAG GACCTGGAAA CACTGAGACC AGACTCGCCT 
GTAGTCTTTG TCGACAAGCC AACAGAGAGG GAGAGAACAG AATGTCTAAT CAAAGCCAAA 
CTCAGGAGTA TCATGATGTG CCAGGACCTT GAAAATGTCA CGTCCAAAGA GATTCGTAAT 
GAGTTGGAGC AGCAGATGAA CTGTAACCTG AAAGAGTACA AAGAGTTCAT AGACAATGAG 
ATGCTACTTA TTCTTGGCCA GATGGATAAA GCCACTCTCA TCTTTGACCA CCTTTATCTG 
GGTTCTGAAT GGAACGCTTC CAATCTAGAG GAACTTCAGG AAAGTGGGGT GGGCCACATC 
TTAAATGTCA CAAGGGAAAT AGATAACTTT TTCCCAGGAA CATTCTGCTA CCACAATGTC 
AGAGTGTATG ATGATGAGGC CACAGACTTA CTGGCTCACT GGAATGATAC CTACAACTTC 
ATTGTCAAAG CAAAAAAGAA TCATTCCAAA TGTTTGGTTC ACTGTAAGAT GGGCGTAAGC 
CGCTCTGCTT CCACGGTGAT CGCATATGCC ATGAAAGAGT ACGGCTGGAC TCTGGAGAAA 
GCATATAACT TTGTTAAACA GAAGCGCAGT ATCACTCGTC CCAATGCCAG CTTCATGAGA 
CAGCTGGCAG AGTATGAAGG CATACTGGAT GCCAGTAAGC AGCGTCATAA CCGTCTGTGG 
CGCCCTGATT CGGATTGCGA TCACCCCGAG TGCCAGCAAG CTGCTGGTCC TTGCTATCTG 
AATGCAGAGA CTGGAGATCA CATCACTCCT GACTCCACAA CCCCCAGGGT CACTGTCTGC 
TGTGAGCCTA CAGCCCACTC CTCTCCAATT AGTCTAGATC TGGATCCTGG CCATCCACAA 
AATTACCACT ACTACTTCCG CCGCTTGTCA GACTCGGCTC TAGAGAGCGA ACCTTCCACC 
CCTGTCCAAG CACAGCCTTT GGTGGATGTA GATCGTGTCT ATATTGAGGT TGCCGATGTG 
GAGCAAGATG CACTTCTTGA GGATGAAACC TTCGAGGCAC ATGAGGGCCC TCCTGTTTCC 
CACTCAGGAG AGGGTATTGG CGCTCAAACA TGTTCTCGAG GACCGGAGCC ACTAGAAGAA 
CTACGTCTGC GGTTGGAGTT CAGCACAGTG GAAGAAGAGG ATGAAGAAGA GGCTCAGAAA 
GAGCAAGCTG AGATGGAGGC GTTAACAGAG GAAGCAAAGA GAGTAGATGG GAAGGATAGT 
GAGGAGCTGT TGAATGAAAA GGGGATCGAT CTGACCAGTG TGAACCATTT GTGCAATGAG 
AACAACAACA ACAATCACCT CAAAATGCAA CAAATCCTCA CTGAAAGTGC CCAAGTAAAG 
ACAAGGCTTG CTCCTGGTTC ATCGCTTGCT TCCAAGCCCA TCCAAAGTGC TCTTTCGGAG 
GCTAACAGAC GAGGCCTTGC CCCTTTACCC CTCAACAGTG CGCCCTGTAT CTCCATTCAG 
ATGCTATCCT GTGCCAACTT GTCCATGACA GCCAAGTGTG GCAGAACTGT CTCAGATCCG 
TGCCTTGATG CCAATGCAAT GTCCTTAGGG AATAACGATA TGCCAGAAAG CAAATCTCTT 
GACCAGGAAA CTGAAACCCA GACAACTCTG CCTGAGGCCA AAGGAGAAGG AGATTTCAGG 
CAGGAAGAGA CTGATCTACA GGGTTCCTCT GCTCTTCCCC GGAATGAGAG AGAGCTGAAA 
GGTTTAGAGA AGGCAGAGGC GTCTTGTTCT CCTCAACAAC AAGAGGCTCT GATTCAGCTC 
CAACGCTCCG GTTTAGTTCG ACGTCGAAAA GAACGATTGG AAAAACTCTC CGGTCTCTTC 
CAGGAGCGTG CACGCGCAAA TGAACAACGG GAAGCTGGTT CTAGTAAAAC AGAAGATGCA 
ATGAACAGTT CCAGTGATCC AGCTGACCCC TTTCACAGGT CTTTTGGAAC ACGGAATGAT 
TTTGGAACTG AATCGGCAGT GCCACTGACC AATGAGGCTT TGTTGGCAGT CCTCGGATCA 
GGAGGATTGA CTCCAGTCTC ATCTCCACAC GGCTCTACGC TCACCCGCAG CTCCAGCAGT 
GACAGTCTTC GCAGTGTCCG AGGCAAACCA GGCCTTGTGC GTCAGCGTAC ACAAGAAATT 
GAAGCACGAA TGCGATTAGC GGGACTGACT GTTCCTTCAC GACTCAAGCG CTCTAACTCT 
CTCGCCAAAC TAGGCAGTCT CACCTTTTCA TCTGAGGACT TGTGCTCGGC CTGCTCCTCT 
GACGCAGGGA CCCTTCTCAT GCTCTCATTG TCCCCAGAAC CCGAGCACTC GCTCAGCCCA 
GAATGGGCAA AACCAGACAA CATGGAGGTC TTCAGCCAAG GCGAAGCAGG ATGTGAAGAG 
TCCAAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh1a ( XM_009301385.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299660.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa51660
Clone ID Related Accession (Same CDS sequence)	XM_009301385.3 , XM_009301385.2
Accession Version	XM_009301385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGTAAGCGGA AACGAGCACG AGGACTTTGG AAGTGACGAT 
GAGCGCCGGC TAAATCAGAG TCTGAGCGAG AGTTTCTTCA TGGTGAAGGG AGCCGCTCTG 
TTTCTGCAGC AGGGCAGTTG TCCACAGGCT CAGAGAGCTC ATCCTCACCA CAAACATGCA 
GGTGACTTGC CTCAGCATCT TCAGGTGATG ATCAACATTC TTCGGTCTGA GGATCGCATC 
AAACTGGCGG TGCGTTTGGA GAGCGCGTGG TCAGACCGTG TGAGGTACAT GGTGGTGGTT 
TACACAAATG GCCGACAAGA CACTGAAGAA AACATCTTAT TGGGCATGGA CTTCAGCAAC 
AAAGACAGTA AGAATTGCTC TGTCGGGATG GTGCTGCCGC TGTGGAGTGA CACCAAGATT 
CATTTAGACG GTGACGGGGG TTTCAGTGTG ACGACCGCCG GGCGTACGCA CATCTTCAAA 
CCGGTGTCAG TCCAGGCCAT GTGGTCAGCT TTGCAAATTT TACACAAGGC CTGTGAGGTG 
TCACGGCGGT ACAACTATTT CCCTGGTGGG ATGGCACTGA CGTGGATGGG GTACTATGAG 
AGCTGCATCA GCTCTGAACA GAGCTGCATC AATGAGTGGA ACGCCATGCA GGACCTGGAA 
ACACTGAGAC CAGACTCGCC TGTAGTCTTT GTCGACAAGC CAACAGAGAG GGAGAGAACA 
GAATGTCTAA TCAAAGCCAA ACTCAGGAGT ATCATGATGT GCCAGGACCT TGAAAATGTC 
ACGTCCAAAG AGATTCGTAA TGAGTTGGAG CAGCAGATGA ACTGTAACCT GAAAGAGTAC 
AAAGAGTTCA TAGACAATGA GATGCTACTT ATTCTTGGCC AGATGGATAA AGCCACTCTC 
ATCTTTGACC ACCTTTATCT GGGTTCTGAA TGGAACGCTT CCAATCTAGA GGAACTTCAG 
GAAAGTGGGG TGGGCCACAT CTTAAATGTC ACAAGGGAAA TAGATAACTT TTTCCCAGGA 
ACATTCTGCT ACCACAATGT CAGAGTGTAT GATGATGAGG CCACAGACTT ACTGGCTCAC 
TGGAATGATA CCTACAACTT CATTGTCAAA GCAAAAAAGA ATCATTCCAA ATGTTTGGTT 
CACTGTAAGA TGGGCGTAAG CCGCTCTGCT TCCACGGTGA TCGCATATGC CATGAAAGAG 
TACGGCTGGA CTCTGGAGAA AGCATATAAC TTTGTTAAAC AGAAGCGCAG TATCACTCGT 
CCCAATGCCA GCTTCATGAG ACAGCTGGCA GAGTATGAAG GCATACTGGA TGCCAGTAAG 
CAGCGTCATA ACCGTCTGTG GCGCCCTGAT TCGGATTGCG ATCACCCCGA GTGCCAGCAA 
GCTGCTGGTC CTTGCTATCT GAATGCAGAG ACTGGAGATC ACATCACTCC TGACTCCACA 
ACCCCCAGGG TCACTGTCTG CTGTGAGCCT ACAGCCCACT CCTCTCCAAT TAGTCTAGAT 
CTGGATCCTG GCCATCCACA AAATTACCAC TACTACTTCC GCCGCTTGTC AGACTCGGCT 
CTAGAGAGCG AACCTTCCAC CCCTGTCCAA GCACAGCCTT TGGTGGATGT AGATCGTGTC 
TATATTGAGG TTGCCGATGT GGAGCAAGAT GCACTTCTTG AGGATGAAAC CTTCGAGGCA 
CATGAGGGCC CTCCTGTTTC CCACTCAGGA GAGGGTATTG GCGCTCAAAC ATGTTCTCGA 
GGACCGGAGC CACTAGAAGA ACTACGTCTG CGGTTGGAGT TCAGCACAGT GGAAGAAGAG 
GATGAAGAAG AGGCTCAGAA AGAGCAAGCT GAGATGGAGG CGTTAACAGA GGAAGCAAAG 
AGAGTAGATG GGAAGGATAG TGAGGAGCTG TTGAATGAAA AGGGGATCGA TCTGACCAGT 
GTGAACCATT TGTGCAATGA GAACAACAAC AACAATCACC TCAAAATGCA ACAAATCCTC 
ACTGAAAGTG CCCAAGTAAA GACAAGGCTT GCTCCTGGTT CATCGCTTGC TTCCAAGCCC 
ATCCAAAGTG CTCTTTCGGA GGCTAACAGA CGAGGCCTTG CCCCTTTACC CCTCAACAGT 
GCGCCCTGTA TCTCCATTCA GATGCTATCC TGTGCCAACT TGTCCATGAC AGCCAAGTGT 
GGCAGAACTG TCTCAGATCC GTGCCTTGAT GCCAATGCAA TGTCCTTAGG GAATAACGAT 
ATGCCAGAAA GCAAATCTCT TGACCAGGAA ACTGAAACCC AGACAACTCT GCCTGAGGCC 
AAAGGAGAAG GAGATTTCAG GCAGGAAGAG ACTGATCTAC AGGGTTCCTC TGCTCTTCCC 
CGGAATGAGA GAGAGCTGAA AGGTTTAGAG AAGGCAGAGG CGTCTTGTTC TCCTCAACAA 
CAAGAGGCTC TGATTCAGCT CCAACGCTCC GGTTTAGTTC GACGTCGAAA AGAACGATTG 
GAAAAACTCT CCGGTCTCTT CCAGGAGCGT GCACGCGCAA ATGAACAACG GGAAGCTGGT 
TCTAGTAAAA CAGAAGATGC AATGAACAGT TCCAGTGATC CAGCTGACCC CTTTCACAGG 
TCTTTTGGAA CACGGAATGA TTTTGGAACT GAATCGGCAG TGCCACTGAC CAATGAGGCT 
TTGTTGGCAG TCCTCGGATC AGGAGGATTG ACTCCAGTCT CATCTCCACA CGGCTCTACG 
CTCACCCGCA GCTCCAGCAG TGACAGTCTT CGCAGTGTCC GAGGCAAACC AGGCCTTGTG 
CGTCAGCGTA CACAAGAAAT TGAAGCACGA ATGCGATTAG CGGGACTGAC TGTTCCTTCA 
CGACTCAAGC GCTCTAACTC TCTCGCCAAA CTAGGCAGTC TCACCTTTTC ATCTGAGGAC 
TTGTGCTCGG CCTGCTCCTC TGACGCAGGG ACCCTTCTCA TGCTCTCATT GTCCCCAGAA 
CCCGAGCACT CGCTCAGCCC AGAATGGGCA AAACCAGACA ACATGGAGGT CTTCAGCCAA 
GGCGAAGCAG GATGTGAAGA GTCCAAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299660.1
CDS	1613..4705
Translation	MALVTLQRSPTPSAASTSSTATTNAGEVSGNEHEDFGSDDERRLNQSLSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_009301385.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301385.3

ORF Insert Sequence:

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGTAAGCGGA AACGAGCACG AGGACTTTGG AAGTGACGAT 
GAGCGCCGGC TAAATCAGAG TCTGAGCGAG AGTTTCTTCA TGGTGAAGGG AGCCGCTCTG 
TTTCTGCAGC AGGGCAGTTG TCCACAGGCT CAGAGAGCTC ATCCTCACCA CAAACATGCA 
GGTGACTTGC CTCAGCATCT TCAGGTGATG ATCAACATTC TTCGGTCTGA GGATCGCATC 
AAACTGGCGG TGCGTTTGGA GAGCGCGTGG TCAGACCGTG TGAGGTACAT GGTGGTGGTT 
TACACAAATG GCCGACAAGA CACTGAAGAA AACATCTTAT TGGGCATGGA CTTCAGCAAC 
AAAGACAGTA AGAATTGCTC TGTCGGGATG GTGCTGCCGC TGTGGAGTGA CACCAAGATT 
CATTTAGACG GTGACGGGGG TTTCAGTGTG ACGACCGCCG GGCGTACGCA CATCTTCAAA 
CCGGTGTCAG TCCAGGCCAT GTGGTCAGCT TTGCAAATTT TACACAAGGC CTGTGAGGTG 
TCACGGCGGT ACAACTATTT CCCTGGTGGG ATGGCACTGA CGTGGATGGG GTACTATGAG 
AGCTGCATCA GCTCTGAACA GAGCTGCATC AATGAGTGGA ACGCCATGCA GGACCTGGAA 
ACACTGAGAC CAGACTCGCC TGTAGTCTTT GTCGACAAGC CAACAGAGAG GGAGAGAACA 
GAATGTCTAA TCAAAGCCAA ACTCAGGAGT ATCATGATGT GCCAGGACCT TGAAAATGTC 
ACGTCCAAAG AGATTCGTAA TGAGTTGGAG CAGCAGATGA ACTGTAACCT GAAAGAGTAC 
AAAGAGTTCA TAGACAATGA GATGCTACTT ATTCTTGGCC AGATGGATAA AGCCACTCTC 
ATCTTTGACC ACCTTTATCT GGGTTCTGAA TGGAACGCTT CCAATCTAGA GGAACTTCAG 
GAAAGTGGGG TGGGCCACAT CTTAAATGTC ACAAGGGAAA TAGATAACTT TTTCCCAGGA 
ACATTCTGCT ACCACAATGT CAGAGTGTAT GATGATGAGG CCACAGACTT ACTGGCTCAC 
TGGAATGATA CCTACAACTT CATTGTCAAA GCAAAAAAGA ATCATTCCAA ATGTTTGGTT 
CACTGTAAGA TGGGCGTAAG CCGCTCTGCT TCCACGGTGA TCGCATATGC CATGAAAGAG 
TACGGCTGGA CTCTGGAGAA AGCATATAAC TTTGTTAAAC AGAAGCGCAG TATCACTCGT 
CCCAATGCCA GCTTCATGAG ACAGCTGGCA GAGTATGAAG GCATACTGGA TGCCAGTAAG 
CAGCGTCATA ACCGTCTGTG GCGCCCTGAT TCGGATTGCG ATCACCCCGA GTGCCAGCAA 
GCTGCTGGTC CTTGCTATCT GAATGCAGAG ACTGGAGATC ACATCACTCC TGACTCCACA 
ACCCCCAGGG TCACTGTCTG CTGTGAGCCT ACAGCCCACT CCTCTCCAAT TAGTCTAGAT 
CTGGATCCTG GCCATCCACA AAATTACCAC TACTACTTCC GCCGCTTGTC AGACTCGGCT 
CTAGAGAGCG AACCTTCCAC CCCTGTCCAA GCACAGCCTT TGGTGGATGT AGATCGTGTC 
TATATTGAGG TTGCCGATGT GGAGCAAGAT GCACTTCTTG AGGATGAAAC CTTCGAGGCA 
CATGAGGGCC CTCCTGTTTC CCACTCAGGA GAGGGTATTG GCGCTCAAAC ATGTTCTCGA 
GGACCGGAGC CACTAGAAGA ACTACGTCTG CGGTTGGAGT TCAGCACAGT GGAAGAAGAG 
GATGAAGAAG AGGCTCAGAA AGAGCAAGCT GAGATGGAGG CGTTAACAGA GGAAGCAAAG 
AGAGTAGATG GGAAGGATAG TGAGGAGCTG TTGAATGAAA AGGGGATCGA TCTGACCAGT 
GTGAACCATT TGTGCAATGA GAACAACAAC AACAATCACC TCAAAATGCA ACAAATCCTC 
ACTGAAAGTG CCCAAGTAAA GACAAGGCTT GCTCCTGGTT CATCGCTTGC TTCCAAGCCC 
ATCCAAAGTG CTCTTTCGGA GGCTAACAGA CGAGGCCTTG CCCCTTTACC CCTCAACAGT 
GCGCCCTGTA TCTCCATTCA GATGCTATCC TGTGCCAACT TGTCCATGAC AGCCAAGTGT 
GGCAGAACTG TCTCAGATCC GTGCCTTGAT GCCAATGCAA TGTCCTTAGG GAATAACGAT 
ATGCCAGAAA GCAAATCTCT TGACCAGGAA ACTGAAACCC AGACAACTCT GCCTGAGGCC 
AAAGGAGAAG GAGATTTCAG GCAGGAAGAG ACTGATCTAC AGGGTTCCTC TGCTCTTCCC 
CGGAATGAGA GAGAGCTGAA AGGTTTAGAG AAGGCAGAGG CGTCTTGTTC TCCTCAACAA 
CAAGAGGCTC TGATTCAGCT CCAACGCTCC GGTTTAGTTC GACGTCGAAA AGAACGATTG 
GAAAAACTCT CCGGTCTCTT CCAGGAGCGT GCACGCGCAA ATGAACAACG GGAAGCTGGT 
TCTAGTAAAA CAGAAGATGC AATGAACAGT TCCAGTGATC CAGCTGACCC CTTTCACAGG 
TCTTTTGGAA CACGGAATGA TTTTGGAACT GAATCGGCAG TGCCACTGAC CAATGAGGCT 
TTGTTGGCAG TCCTCGGATC AGGAGGATTG ACTCCAGTCT CATCTCCACA CGGCTCTACG 
CTCACCCGCA GCTCCAGCAG TGACAGTCTT CGCAGTGTCC GAGGCAAACC AGGCCTTGTG 
CGTCAGCGTA CACAAGAAAT TGAAGCACGA ATGCGATTAG CGGGACTGAC TGTTCCTTCA 
CGACTCAAGC GCTCTAACTC TCTCGCCAAA CTAGGCAGTC TCACCTTTTC ATCTGAGGAC 
TTGTGCTCGG CCTGCTCCTC TGACGCAGGG ACCCTTCTCA TGCTCTCATT GTCCCCAGAA 
CCCGAGCACT CGCTCAGCCC AGAATGGGCA AAACCAGACA ACATGGAGGT CTTCAGCCAA 
GGCGAAGCAG GATGTGAAGA GTCCAAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh1a ( XM_009301385.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299660.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa51660
Clone ID Related Accession (Same CDS sequence)	XM_009301385.3 , XM_009301385.2
Accession Version	XM_009301385.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGTAAGCGGA AACGAGCACG AGGACTTTGG AAGTGACGAT 
GAGCGCCGGC TAAATCAGAG TCTGAGCGAG AGTTTCTTCA TGGTGAAGGG AGCCGCTCTG 
TTTCTGCAGC AGGGCAGTTG TCCACAGGCT CAGAGAGCTC ATCCTCACCA CAAACATGCA 
GGTGACTTGC CTCAGCATCT TCAGGTGATG ATCAACATTC TTCGGTCTGA GGATCGCATC 
AAACTGGCGG TGCGTTTGGA GAGCGCGTGG TCAGACCGTG TGAGGTACAT GGTGGTGGTT 
TACACAAATG GCCGACAAGA CACTGAAGAA AACATCTTAT TGGGCATGGA CTTCAGCAAC 
AAAGACAGTA AGAATTGCTC TGTCGGGATG GTGCTGCCGC TGTGGAGTGA CACCAAGATT 
CATTTAGACG GTGACGGGGG TTTCAGTGTG ACGACCGCCG GGCGTACGCA CATCTTCAAA 
CCGGTGTCAG TCCAGGCCAT GTGGTCAGCT TTGCAAATTT TACACAAGGC CTGTGAGGTG 
TCACGGCGGT ACAACTATTT CCCTGGTGGG ATGGCACTGA CGTGGATGGG GTACTATGAG 
AGCTGCATCA GCTCTGAACA GAGCTGCATC AATGAGTGGA ACGCCATGCA GGACCTGGAA 
ACACTGAGAC CAGACTCGCC TGTAGTCTTT GTCGACAAGC CAACAGAGAG GGAGAGAACA 
GAATGTCTAA TCAAAGCCAA ACTCAGGAGT ATCATGATGT GCCAGGACCT TGAAAATGTC 
ACGTCCAAAG AGATTCGTAA TGAGTTGGAG CAGCAGATGA ACTGTAACCT GAAAGAGTAC 
AAAGAGTTCA TAGACAATGA GATGCTACTT ATTCTTGGCC AGATGGATAA AGCCACTCTC 
ATCTTTGACC ACCTTTATCT GGGTTCTGAA TGGAACGCTT CCAATCTAGA GGAACTTCAG 
GAAAGTGGGG TGGGCCACAT CTTAAATGTC ACAAGGGAAA TAGATAACTT TTTCCCAGGA 
ACATTCTGCT ACCACAATGT CAGAGTGTAT GATGATGAGG CCACAGACTT ACTGGCTCAC 
TGGAATGATA CCTACAACTT CATTGTCAAA GCAAAAAAGA ATCATTCCAA ATGTTTGGTT 
CACTGTAAGA TGGGCGTAAG CCGCTCTGCT TCCACGGTGA TCGCATATGC CATGAAAGAG 
TACGGCTGGA CTCTGGAGAA AGCATATAAC TTTGTTAAAC AGAAGCGCAG TATCACTCGT 
CCCAATGCCA GCTTCATGAG ACAGCTGGCA GAGTATGAAG GCATACTGGA TGCCAGTAAG 
CAGCGTCATA ACCGTCTGTG GCGCCCTGAT TCGGATTGCG ATCACCCCGA GTGCCAGCAA 
GCTGCTGGTC CTTGCTATCT GAATGCAGAG ACTGGAGATC ACATCACTCC TGACTCCACA 
ACCCCCAGGG TCACTGTCTG CTGTGAGCCT ACAGCCCACT CCTCTCCAAT TAGTCTAGAT 
CTGGATCCTG GCCATCCACA AAATTACCAC TACTACTTCC GCCGCTTGTC AGACTCGGCT 
CTAGAGAGCG AACCTTCCAC CCCTGTCCAA GCACAGCCTT TGGTGGATGT AGATCGTGTC 
TATATTGAGG TTGCCGATGT GGAGCAAGAT GCACTTCTTG AGGATGAAAC CTTCGAGGCA 
CATGAGGGCC CTCCTGTTTC CCACTCAGGA GAGGGTATTG GCGCTCAAAC ATGTTCTCGA 
GGACCGGAGC CACTAGAAGA ACTACGTCTG CGGTTGGAGT TCAGCACAGT GGAAGAAGAG 
GATGAAGAAG AGGCTCAGAA AGAGCAAGCT GAGATGGAGG CGTTAACAGA GGAAGCAAAG 
AGAGTAGATG GGAAGGATAG TGAGGAGCTG TTGAATGAAA AGGGGATCGA TCTGACCAGT 
GTGAACCATT TGTGCAATGA GAACAACAAC AACAATCACC TCAAAATGCA ACAAATCCTC 
ACTGAAAGTG CCCAAGTAAA GACAAGGCTT GCTCCTGGTT CATCGCTTGC TTCCAAGCCC 
ATCCAAAGTG CTCTTTCGGA GGCTAACAGA CGAGGCCTTG CCCCTTTACC CCTCAACAGT 
GCGCCCTGTA TCTCCATTCA GATGCTATCC TGTGCCAACT TGTCCATGAC AGCCAAGTGT 
GGCAGAACTG TCTCAGATCC GTGCCTTGAT GCCAATGCAA TGTCCTTAGG GAATAACGAT 
ATGCCAGAAA GCAAATCTCT TGACCAGGAA ACTGAAACCC AGACAACTCT GCCTGAGGCC 
AAAGGAGAAG GAGATTTCAG GCAGGAAGAG ACTGATCTAC AGGGTTCCTC TGCTCTTCCC 
CGGAATGAGA GAGAGCTGAA AGGTTTAGAG AAGGCAGAGG CGTCTTGTTC TCCTCAACAA 
CAAGAGGCTC TGATTCAGCT CCAACGCTCC GGTTTAGTTC GACGTCGAAA AGAACGATTG 
GAAAAACTCT CCGGTCTCTT CCAGGAGCGT GCACGCGCAA ATGAACAACG GGAAGCTGGT 
TCTAGTAAAA CAGAAGATGC AATGAACAGT TCCAGTGATC CAGCTGACCC CTTTCACAGG 
TCTTTTGGAA CACGGAATGA TTTTGGAACT GAATCGGCAG TGCCACTGAC CAATGAGGCT 
TTGTTGGCAG TCCTCGGATC AGGAGGATTG ACTCCAGTCT CATCTCCACA CGGCTCTACG 
CTCACCCGCA GCTCCAGCAG TGACAGTCTT CGCAGTGTCC GAGGCAAACC AGGCCTTGTG 
CGTCAGCGTA CACAAGAAAT TGAAGCACGA ATGCGATTAG CGGGACTGAC TGTTCCTTCA 
CGACTCAAGC GCTCTAACTC TCTCGCCAAA CTAGGCAGTC TCACCTTTTC ATCTGAGGAC 
TTGTGCTCGG CCTGCTCCTC TGACGCAGGG ACCCTTCTCA TGCTCTCATT GTCCCCAGAA 
CCCGAGCACT CGCTCAGCCC AGAATGGGCA AAACCAGACA ACATGGAGGT CTTCAGCCAA 
GGCGAAGCAG GATGTGAAGA GTCCAAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299660.1
CDS	1603..4695
Translation	MALVTLQRSPTPSAASTSSTATTNAGEVSGNEHEDFGSDDERRLNQSLSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_009301385.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301385.2

ORF Insert Sequence:

ATGGCTCTGG TCACTCTGCA GCGCTCGCCC ACTCCCAGCG CCGCGTCCAC TTCCAGCACC 
GCCACCACCA ACGCGGGGGA GGTAAGCGGA AACGAGCACG AGGACTTTGG AAGTGACGAT 
GAGCGCCGGC TAAATCAGAG TCTGAGCGAG AGTTTCTTCA TGGTGAAGGG AGCCGCTCTG 
TTTCTGCAGC AGGGCAGTTG TCCACAGGCT CAGAGAGCTC ATCCTCACCA CAAACATGCA 
GGTGACTTGC CTCAGCATCT TCAGGTGATG ATCAACATTC TTCGGTCTGA GGATCGCATC 
AAACTGGCGG TGCGTTTGGA GAGCGCGTGG TCAGACCGTG TGAGGTACAT GGTGGTGGTT 
TACACAAATG GCCGACAAGA CACTGAAGAA AACATCTTAT TGGGCATGGA CTTCAGCAAC 
AAAGACAGTA AGAATTGCTC TGTCGGGATG GTGCTGCCGC TGTGGAGTGA CACCAAGATT 
CATTTAGACG GTGACGGGGG TTTCAGTGTG ACGACCGCCG GGCGTACGCA CATCTTCAAA 
CCGGTGTCAG TCCAGGCCAT GTGGTCAGCT TTGCAAATTT TACACAAGGC CTGTGAGGTG 
TCACGGCGGT ACAACTATTT CCCTGGTGGG ATGGCACTGA CGTGGATGGG GTACTATGAG 
AGCTGCATCA GCTCTGAACA GAGCTGCATC AATGAGTGGA ACGCCATGCA GGACCTGGAA 
ACACTGAGAC CAGACTCGCC TGTAGTCTTT GTCGACAAGC CAACAGAGAG GGAGAGAACA 
GAATGTCTAA TCAAAGCCAA ACTCAGGAGT ATCATGATGT GCCAGGACCT TGAAAATGTC 
ACGTCCAAAG AGATTCGTAA TGAGTTGGAG CAGCAGATGA ACTGTAACCT GAAAGAGTAC 
AAAGAGTTCA TAGACAATGA GATGCTACTT ATTCTTGGCC AGATGGATAA AGCCACTCTC 
ATCTTTGACC ACCTTTATCT GGGTTCTGAA TGGAACGCTT CCAATCTAGA GGAACTTCAG 
GAAAGTGGGG TGGGCCACAT CTTAAATGTC ACAAGGGAAA TAGATAACTT TTTCCCAGGA 
ACATTCTGCT ACCACAATGT CAGAGTGTAT GATGATGAGG CCACAGACTT ACTGGCTCAC 
TGGAATGATA CCTACAACTT CATTGTCAAA GCAAAAAAGA ATCATTCCAA ATGTTTGGTT 
CACTGTAAGA TGGGCGTAAG CCGCTCTGCT TCCACGGTGA TCGCATATGC CATGAAAGAG 
TACGGCTGGA CTCTGGAGAA AGCATATAAC TTTGTTAAAC AGAAGCGCAG TATCACTCGT 
CCCAATGCCA GCTTCATGAG ACAGCTGGCA GAGTATGAAG GCATACTGGA TGCCAGTAAG 
CAGCGTCATA ACCGTCTGTG GCGCCCTGAT TCGGATTGCG ATCACCCCGA GTGCCAGCAA 
GCTGCTGGTC CTTGCTATCT GAATGCAGAG ACTGGAGATC ACATCACTCC TGACTCCACA 
ACCCCCAGGG TCACTGTCTG CTGTGAGCCT ACAGCCCACT CCTCTCCAAT TAGTCTAGAT 
CTGGATCCTG GCCATCCACA AAATTACCAC TACTACTTCC GCCGCTTGTC AGACTCGGCT 
CTAGAGAGCG AACCTTCCAC CCCTGTCCAA GCACAGCCTT TGGTGGATGT AGATCGTGTC 
TATATTGAGG TTGCCGATGT GGAGCAAGAT GCACTTCTTG AGGATGAAAC CTTCGAGGCA 
CATGAGGGCC CTCCTGTTTC CCACTCAGGA GAGGGTATTG GCGCTCAAAC ATGTTCTCGA 
GGACCGGAGC CACTAGAAGA ACTACGTCTG CGGTTGGAGT TCAGCACAGT GGAAGAAGAG 
GATGAAGAAG AGGCTCAGAA AGAGCAAGCT GAGATGGAGG CGTTAACAGA GGAAGCAAAG 
AGAGTAGATG GGAAGGATAG TGAGGAGCTG TTGAATGAAA AGGGGATCGA TCTGACCAGT 
GTGAACCATT TGTGCAATGA GAACAACAAC AACAATCACC TCAAAATGCA ACAAATCCTC 
ACTGAAAGTG CCCAAGTAAA GACAAGGCTT GCTCCTGGTT CATCGCTTGC TTCCAAGCCC 
ATCCAAAGTG CTCTTTCGGA GGCTAACAGA CGAGGCCTTG CCCCTTTACC CCTCAACAGT 
GCGCCCTGTA TCTCCATTCA GATGCTATCC TGTGCCAACT TGTCCATGAC AGCCAAGTGT 
GGCAGAACTG TCTCAGATCC GTGCCTTGAT GCCAATGCAA TGTCCTTAGG GAATAACGAT 
ATGCCAGAAA GCAAATCTCT TGACCAGGAA ACTGAAACCC AGACAACTCT GCCTGAGGCC 
AAAGGAGAAG GAGATTTCAG GCAGGAAGAG ACTGATCTAC AGGGTTCCTC TGCTCTTCCC 
CGGAATGAGA GAGAGCTGAA AGGTTTAGAG AAGGCAGAGG CGTCTTGTTC TCCTCAACAA 
CAAGAGGCTC TGATTCAGCT CCAACGCTCC GGTTTAGTTC GACGTCGAAA AGAACGATTG 
GAAAAACTCT CCGGTCTCTT CCAGGAGCGT GCACGCGCAA ATGAACAACG GGAAGCTGGT 
TCTAGTAAAA CAGAAGATGC AATGAACAGT TCCAGTGATC CAGCTGACCC CTTTCACAGG 
TCTTTTGGAA CACGGAATGA TTTTGGAACT GAATCGGCAG TGCCACTGAC CAATGAGGCT 
TTGTTGGCAG TCCTCGGATC AGGAGGATTG ACTCCAGTCT CATCTCCACA CGGCTCTACG 
CTCACCCGCA GCTCCAGCAG TGACAGTCTT CGCAGTGTCC GAGGCAAACC AGGCCTTGTG 
CGTCAGCGTA CACAAGAAAT TGAAGCACGA ATGCGATTAG CGGGACTGAC TGTTCCTTCA 
CGACTCAAGC GCTCTAACTC TCTCGCCAAA CTAGGCAGTC TCACCTTTTC ATCTGAGGAC 
TTGTGCTCGG CCTGCTCCTC TGACGCAGGG ACCCTTCTCA TGCTCTCATT GTCCCCAGAA 
CCCGAGCACT CGCTCAGCCC AGAATGGGCA AAACCAGACA ACATGGAGGT CTTCAGCCAA 
GGCGAAGCAG GATGTGAAGA GTCCAAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh1a ( XM_009301386.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299661.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa51661
Clone ID Related Accession (Same CDS sequence)	XM_009301386.2 , XM_009301386.3
Accession Version	XM_009301386.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTAT TCGGGGACTC CTGTCAGGAA GTTGTGCTGA AGATGCTGCC GTATTTTGCG 
GATAATGCTT TCGTCACGCA AAGCCAGATC TACCACATTC TGAGCGAGAG TTTCTTCATG 
GTGAAGGGAG CCGCTCTGTT TCTGCAGCAG GGCAGTTGTC CACAGGCTCA GAGAGCTCAT 
CCTCACCACA AACATGCAGG TGACTTGCCT CAGCATCTTC AGGTGATGAT CAACATTCTT 
CGGTCTGAGG ATCGCATCAA ACTGGCGGTG CGTTTGGAGA GCGCGTGGTC AGACCGTGTG 
AGGTACATGG TGGTGGTTTA CACAAATGGC CGACAAGACA CTGAAGAAAA CATCTTATTG 
GGCATGGACT TCAGCAACAA AGACAGTAAG AATTGCTCTG TCGGGATGGT GCTGCCGCTG 
TGGAGTGACA CCAAGATTCA TTTAGACGGT GACGGGGGTT TCAGTGTGAC GACCGCCGGG 
CGTACGCACA TCTTCAAACC GGTGTCAGTC CAGGCCATGT GGTCAGCTTT GCAAATTTTA 
CACAAGGCCT GTGAGGTGTC ACGGCGGTAC AACTATTTCC CTGGTGGGAT GGCACTGACG 
TGGATGGGGT ACTATGAGAG CTGCATCAGC TCTGAACAGA GCTGCATCAA TGAGTGGAAC 
GCCATGCAGG ACCTGGAAAC ACTGAGACCA GACTCGCCTG TAGTCTTTGT CGACAAGCCA 
ACAGAGAGGG AGAGAACAGA ATGTCTAATC AAAGCCAAAC TCAGGAGTAT CATGATGTGC 
CAGGACCTTG AAAATGTCAC GTCCAAAGAG ATTCGTAATG AGTTGGAGCA GCAGATGAAC 
TGTAACCTGA AAGAGTACAA AGAGTTCATA GACAATGAGA TGCTACTTAT TCTTGGCCAG 
ATGGATAAAG CCACTCTCAT CTTTGACCAC CTTTATCTGG GTTCTGAATG GAACGCTTCC 
AATCTAGAGG AACTTCAGGA AAGTGGGGTG GGCCACATCT TAAATGTCAC AAGGGAAATA 
GATAACTTTT TCCCAGGAAC ATTCTGCTAC CACAATGTCA GAGTGTATGA TGATGAGGCC 
ACAGACTTAC TGGCTCACTG GAATGATACC TACAACTTCA TTGTCAAAGC AAAAAAGAAT 
CATTCCAAAT GTTTGGTTCA CTGTAAGATG GGCGTAAGCC GCTCTGCTTC CACGGTGATC 
GCATATGCCA TGAAAGAGTA CGGCTGGACT CTGGAGAAAG CATATAACTT TGTTAAACAG 
AAGCGCAGTA TCACTCGTCC CAATGCCAGC TTCATGAGAC AGCTGGCAGA GTATGAAGGC 
ATACTGGATG CCAGTAAGCA GCGTCATAAC CGTCTGTGGC GCCCTGATTC GGATTGCGAT 
CACCCCGAGT GCCAGCAAGC TGCTGGTCCT TGCTATCTGA ATGCAGAGAC TGGAGATCAC 
ATCACTCCTG ACTCCACAAC CCCCAGGGTC ACTGTCTGCT GTGAGCCTAC AGCCCACTCC 
TCTCCAATTA GTCTAGATCT GGATCCTGGC CATCCACAAA ATTACCACTA CTACTTCCGC 
CGCTTGTCAG ACTCGGCTCT AGAGAGCGAA CCTTCCACCC CTGTCCAAGC ACAGCCTTTG 
GTGGATGTAG ATCGTGTCTA TATTGAGGTT GCCGATGTGG AGCAAGATGC ACTTCTTGAG 
GATGAAACCT TCGAGGCACA TGAGGGCCCT CCTGTTTCCC ACTCAGGAGA GGGTATTGGC 
GCTCAAACAT GTTCTCGAGG ACCGGAGCCA CTAGAAGAAC TACGTCTGCG GTTGGAGTTC 
AGCACAGTGG AAGAAGAGGA TGAAGAAGAG GCTCAGAAAG AGCAAGCTGA GATGGAGGCG 
TTAACAGAGG AAGCAAAGAG AGTAGATGGG AAGGATAGTG AGGAGCTGTT GAATGAAAAG 
GGGATCGATC TGACCAGTGT GAACCATTTG TGCAATGAGA ACAACAACAA CAATCACCTC 
AAAATGCAAC AAATCCTCAC TGAAAGTGCC CAAGTAAAGA CAAGGCTTGC TCCTGGTTCA 
TCGCTTGCTT CCAAGCCCAT CCAAAGTGCT CTTTCGGAGG CTAACAGACG AGGCCTTGCC 
CCTTTACCCC TCAACAGTGC GCCCTGTATC TCCATTCAGA TGCTATCCTG TGCCAACTTG 
TCCATGACAG CCAAGTGTGG CAGAACTGTC TCAGATCCGT GCCTTGATGC CAATGCAATG 
TCCTTAGGGA ATAACGATAT GCCAGAAAGC AAATCTCTTG ACCAGGAAAC TGAAACCCAG 
ACAACTCTGC CTGAGGCCAA AGGAGAAGGA GATTTCAGGC AGGAAGAGAC TGATCTACAG 
GGTTCCTCTG CTCTTCCCCG GAATGAGAGA GAGCTGAAAG GTTTAGAGAA GGCAGAGGCG 
TCTTGTTCTC CTCAACAACA AGAGGCTCTG ATTCAGCTCC AACGCTCCGG TTTAGTTCGA 
CGTCGAAAAG AACGATTGGA AAAACTCTCC GGTCTCTTCC AGGAGCGTGC ACGCGCAAAT 
GAACAACGGG AAGCTGGTTC TAGTAAAACA GAAGATGCAA TGAACAGTTC CAGTGATCCA 
GCTGACCCCT TTCACAGGTC TTTTGGAACA CGGAATGATT TTGGAACTGA ATCGGCAGTG 
CCACTGACCA ATGAGGCTTT GTTGGCAGTC CTCGGATCAG GAGGATTGAC TCCAGTCTCA 
TCTCCACACG GCTCTACGCT CACCCGCAGC TCCAGCAGTG ACAGTCTTCG CAGTGTCCGA 
GGCAAACCAG GCCTTGTGCG TCAGCGTACA CAAGAAATTG AAGCACGAAT GCGATTAGCG 
GGACTGACTG TTCCTTCACG ACTCAAGCGC TCTAACTCTC TCGCCAAACT AGGCAGTCTC 
ACCTTTTCAT CTGAGGACTT GTGCTCGGCC TGCTCCTCTG ACGCAGGGAC CCTTCTCATG 
CTCTCATTGT CCCCAGAACC CGAGCACTCG CTCAGCCCAG AATGGGCAAA ACCAGACAAC 
ATGGAGGTCT TCAGCCAAGG CGAAGCAGGA TGTGAAGAGT CCAAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299661.1
CDS	125..3175
Translation	MHLFGDSCQEVVLKMLPYFADNAFVTQSQIYHILSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_009301386.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301386.2

ORF Insert Sequence:

ATGCATCTAT TCGGGGACTC CTGTCAGGAA GTTGTGCTGA AGATGCTGCC GTATTTTGCG 
GATAATGCTT TCGTCACGCA AAGCCAGATC TACCACATTC TGAGCGAGAG TTTCTTCATG 
GTGAAGGGAG CCGCTCTGTT TCTGCAGCAG GGCAGTTGTC CACAGGCTCA GAGAGCTCAT 
CCTCACCACA AACATGCAGG TGACTTGCCT CAGCATCTTC AGGTGATGAT CAACATTCTT 
CGGTCTGAGG ATCGCATCAA ACTGGCGGTG CGTTTGGAGA GCGCGTGGTC AGACCGTGTG 
AGGTACATGG TGGTGGTTTA CACAAATGGC CGACAAGACA CTGAAGAAAA CATCTTATTG 
GGCATGGACT TCAGCAACAA AGACAGTAAG AATTGCTCTG TCGGGATGGT GCTGCCGCTG 
TGGAGTGACA CCAAGATTCA TTTAGACGGT GACGGGGGTT TCAGTGTGAC GACCGCCGGG 
CGTACGCACA TCTTCAAACC GGTGTCAGTC CAGGCCATGT GGTCAGCTTT GCAAATTTTA 
CACAAGGCCT GTGAGGTGTC ACGGCGGTAC AACTATTTCC CTGGTGGGAT GGCACTGACG 
TGGATGGGGT ACTATGAGAG CTGCATCAGC TCTGAACAGA GCTGCATCAA TGAGTGGAAC 
GCCATGCAGG ACCTGGAAAC ACTGAGACCA GACTCGCCTG TAGTCTTTGT CGACAAGCCA 
ACAGAGAGGG AGAGAACAGA ATGTCTAATC AAAGCCAAAC TCAGGAGTAT CATGATGTGC 
CAGGACCTTG AAAATGTCAC GTCCAAAGAG ATTCGTAATG AGTTGGAGCA GCAGATGAAC 
TGTAACCTGA AAGAGTACAA AGAGTTCATA GACAATGAGA TGCTACTTAT TCTTGGCCAG 
ATGGATAAAG CCACTCTCAT CTTTGACCAC CTTTATCTGG GTTCTGAATG GAACGCTTCC 
AATCTAGAGG AACTTCAGGA AAGTGGGGTG GGCCACATCT TAAATGTCAC AAGGGAAATA 
GATAACTTTT TCCCAGGAAC ATTCTGCTAC CACAATGTCA GAGTGTATGA TGATGAGGCC 
ACAGACTTAC TGGCTCACTG GAATGATACC TACAACTTCA TTGTCAAAGC AAAAAAGAAT 
CATTCCAAAT GTTTGGTTCA CTGTAAGATG GGCGTAAGCC GCTCTGCTTC CACGGTGATC 
GCATATGCCA TGAAAGAGTA CGGCTGGACT CTGGAGAAAG CATATAACTT TGTTAAACAG 
AAGCGCAGTA TCACTCGTCC CAATGCCAGC TTCATGAGAC AGCTGGCAGA GTATGAAGGC 
ATACTGGATG CCAGTAAGCA GCGTCATAAC CGTCTGTGGC GCCCTGATTC GGATTGCGAT 
CACCCCGAGT GCCAGCAAGC TGCTGGTCCT TGCTATCTGA ATGCAGAGAC TGGAGATCAC 
ATCACTCCTG ACTCCACAAC CCCCAGGGTC ACTGTCTGCT GTGAGCCTAC AGCCCACTCC 
TCTCCAATTA GTCTAGATCT GGATCCTGGC CATCCACAAA ATTACCACTA CTACTTCCGC 
CGCTTGTCAG ACTCGGCTCT AGAGAGCGAA CCTTCCACCC CTGTCCAAGC ACAGCCTTTG 
GTGGATGTAG ATCGTGTCTA TATTGAGGTT GCCGATGTGG AGCAAGATGC ACTTCTTGAG 
GATGAAACCT TCGAGGCACA TGAGGGCCCT CCTGTTTCCC ACTCAGGAGA GGGTATTGGC 
GCTCAAACAT GTTCTCGAGG ACCGGAGCCA CTAGAAGAAC TACGTCTGCG GTTGGAGTTC 
AGCACAGTGG AAGAAGAGGA TGAAGAAGAG GCTCAGAAAG AGCAAGCTGA GATGGAGGCG 
TTAACAGAGG AAGCAAAGAG AGTAGATGGG AAGGATAGTG AGGAGCTGTT GAATGAAAAG 
GGGATCGATC TGACCAGTGT GAACCATTTG TGCAATGAGA ACAACAACAA CAATCACCTC 
AAAATGCAAC AAATCCTCAC TGAAAGTGCC CAAGTAAAGA CAAGGCTTGC TCCTGGTTCA 
TCGCTTGCTT CCAAGCCCAT CCAAAGTGCT CTTTCGGAGG CTAACAGACG AGGCCTTGCC 
CCTTTACCCC TCAACAGTGC GCCCTGTATC TCCATTCAGA TGCTATCCTG TGCCAACTTG 
TCCATGACAG CCAAGTGTGG CAGAACTGTC TCAGATCCGT GCCTTGATGC CAATGCAATG 
TCCTTAGGGA ATAACGATAT GCCAGAAAGC AAATCTCTTG ACCAGGAAAC TGAAACCCAG 
ACAACTCTGC CTGAGGCCAA AGGAGAAGGA GATTTCAGGC AGGAAGAGAC TGATCTACAG 
GGTTCCTCTG CTCTTCCCCG GAATGAGAGA GAGCTGAAAG GTTTAGAGAA GGCAGAGGCG 
TCTTGTTCTC CTCAACAACA AGAGGCTCTG ATTCAGCTCC AACGCTCCGG TTTAGTTCGA 
CGTCGAAAAG AACGATTGGA AAAACTCTCC GGTCTCTTCC AGGAGCGTGC ACGCGCAAAT 
GAACAACGGG AAGCTGGTTC TAGTAAAACA GAAGATGCAA TGAACAGTTC CAGTGATCCA 
GCTGACCCCT TTCACAGGTC TTTTGGAACA CGGAATGATT TTGGAACTGA ATCGGCAGTG 
CCACTGACCA ATGAGGCTTT GTTGGCAGTC CTCGGATCAG GAGGATTGAC TCCAGTCTCA 
TCTCCACACG GCTCTACGCT CACCCGCAGC TCCAGCAGTG ACAGTCTTCG CAGTGTCCGA 
GGCAAACCAG GCCTTGTGCG TCAGCGTACA CAAGAAATTG AAGCACGAAT GCGATTAGCG 
GGACTGACTG TTCCTTCACG ACTCAAGCGC TCTAACTCTC TCGCCAAACT AGGCAGTCTC 
ACCTTTTCAT CTGAGGACTT GTGCTCGGCC TGCTCCTCTG ACGCAGGGAC CCTTCTCATG 
CTCTCATTGT CCCCAGAACC CGAGCACTCG CTCAGCCCAG AATGGGCAAA ACCAGACAAC 
ATGGAGGTCT TCAGCCAAGG CGAAGCAGGA TGTGAAGAGT CCAAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh1a ( XM_009301386.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299661.1 Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa51661
Clone ID Related Accession (Same CDS sequence)	XM_009301386.2 , XM_009301386.3
Accession Version	XM_009301386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTAT TCGGGGACTC CTGTCAGGAA GTTGTGCTGA AGATGCTGCC GTATTTTGCG 
GATAATGCTT TCGTCACGCA AAGCCAGATC TACCACATTC TGAGCGAGAG TTTCTTCATG 
GTGAAGGGAG CCGCTCTGTT TCTGCAGCAG GGCAGTTGTC CACAGGCTCA GAGAGCTCAT 
CCTCACCACA AACATGCAGG TGACTTGCCT CAGCATCTTC AGGTGATGAT CAACATTCTT 
CGGTCTGAGG ATCGCATCAA ACTGGCGGTG CGTTTGGAGA GCGCGTGGTC AGACCGTGTG 
AGGTACATGG TGGTGGTTTA CACAAATGGC CGACAAGACA CTGAAGAAAA CATCTTATTG 
GGCATGGACT TCAGCAACAA AGACAGTAAG AATTGCTCTG TCGGGATGGT GCTGCCGCTG 
TGGAGTGACA CCAAGATTCA TTTAGACGGT GACGGGGGTT TCAGTGTGAC GACCGCCGGG 
CGTACGCACA TCTTCAAACC GGTGTCAGTC CAGGCCATGT GGTCAGCTTT GCAAATTTTA 
CACAAGGCCT GTGAGGTGTC ACGGCGGTAC AACTATTTCC CTGGTGGGAT GGCACTGACG 
TGGATGGGGT ACTATGAGAG CTGCATCAGC TCTGAACAGA GCTGCATCAA TGAGTGGAAC 
GCCATGCAGG ACCTGGAAAC ACTGAGACCA GACTCGCCTG TAGTCTTTGT CGACAAGCCA 
ACAGAGAGGG AGAGAACAGA ATGTCTAATC AAAGCCAAAC TCAGGAGTAT CATGATGTGC 
CAGGACCTTG AAAATGTCAC GTCCAAAGAG ATTCGTAATG AGTTGGAGCA GCAGATGAAC 
TGTAACCTGA AAGAGTACAA AGAGTTCATA GACAATGAGA TGCTACTTAT TCTTGGCCAG 
ATGGATAAAG CCACTCTCAT CTTTGACCAC CTTTATCTGG GTTCTGAATG GAACGCTTCC 
AATCTAGAGG AACTTCAGGA AAGTGGGGTG GGCCACATCT TAAATGTCAC AAGGGAAATA 
GATAACTTTT TCCCAGGAAC ATTCTGCTAC CACAATGTCA GAGTGTATGA TGATGAGGCC 
ACAGACTTAC TGGCTCACTG GAATGATACC TACAACTTCA TTGTCAAAGC AAAAAAGAAT 
CATTCCAAAT GTTTGGTTCA CTGTAAGATG GGCGTAAGCC GCTCTGCTTC CACGGTGATC 
GCATATGCCA TGAAAGAGTA CGGCTGGACT CTGGAGAAAG CATATAACTT TGTTAAACAG 
AAGCGCAGTA TCACTCGTCC CAATGCCAGC TTCATGAGAC AGCTGGCAGA GTATGAAGGC 
ATACTGGATG CCAGTAAGCA GCGTCATAAC CGTCTGTGGC GCCCTGATTC GGATTGCGAT 
CACCCCGAGT GCCAGCAAGC TGCTGGTCCT TGCTATCTGA ATGCAGAGAC TGGAGATCAC 
ATCACTCCTG ACTCCACAAC CCCCAGGGTC ACTGTCTGCT GTGAGCCTAC AGCCCACTCC 
TCTCCAATTA GTCTAGATCT GGATCCTGGC CATCCACAAA ATTACCACTA CTACTTCCGC 
CGCTTGTCAG ACTCGGCTCT AGAGAGCGAA CCTTCCACCC CTGTCCAAGC ACAGCCTTTG 
GTGGATGTAG ATCGTGTCTA TATTGAGGTT GCCGATGTGG AGCAAGATGC ACTTCTTGAG 
GATGAAACCT TCGAGGCACA TGAGGGCCCT CCTGTTTCCC ACTCAGGAGA GGGTATTGGC 
GCTCAAACAT GTTCTCGAGG ACCGGAGCCA CTAGAAGAAC TACGTCTGCG GTTGGAGTTC 
AGCACAGTGG AAGAAGAGGA TGAAGAAGAG GCTCAGAAAG AGCAAGCTGA GATGGAGGCG 
TTAACAGAGG AAGCAAAGAG AGTAGATGGG AAGGATAGTG AGGAGCTGTT GAATGAAAAG 
GGGATCGATC TGACCAGTGT GAACCATTTG TGCAATGAGA ACAACAACAA CAATCACCTC 
AAAATGCAAC AAATCCTCAC TGAAAGTGCC CAAGTAAAGA CAAGGCTTGC TCCTGGTTCA 
TCGCTTGCTT CCAAGCCCAT CCAAAGTGCT CTTTCGGAGG CTAACAGACG AGGCCTTGCC 
CCTTTACCCC TCAACAGTGC GCCCTGTATC TCCATTCAGA TGCTATCCTG TGCCAACTTG 
TCCATGACAG CCAAGTGTGG CAGAACTGTC TCAGATCCGT GCCTTGATGC CAATGCAATG 
TCCTTAGGGA ATAACGATAT GCCAGAAAGC AAATCTCTTG ACCAGGAAAC TGAAACCCAG 
ACAACTCTGC CTGAGGCCAA AGGAGAAGGA GATTTCAGGC AGGAAGAGAC TGATCTACAG 
GGTTCCTCTG CTCTTCCCCG GAATGAGAGA GAGCTGAAAG GTTTAGAGAA GGCAGAGGCG 
TCTTGTTCTC CTCAACAACA AGAGGCTCTG ATTCAGCTCC AACGCTCCGG TTTAGTTCGA 
CGTCGAAAAG AACGATTGGA AAAACTCTCC GGTCTCTTCC AGGAGCGTGC ACGCGCAAAT 
GAACAACGGG AAGCTGGTTC TAGTAAAACA GAAGATGCAA TGAACAGTTC CAGTGATCCA 
GCTGACCCCT TTCACAGGTC TTTTGGAACA CGGAATGATT TTGGAACTGA ATCGGCAGTG 
CCACTGACCA ATGAGGCTTT GTTGGCAGTC CTCGGATCAG GAGGATTGAC TCCAGTCTCA 
TCTCCACACG GCTCTACGCT CACCCGCAGC TCCAGCAGTG ACAGTCTTCG CAGTGTCCGA 
GGCAAACCAG GCCTTGTGCG TCAGCGTACA CAAGAAATTG AAGCACGAAT GCGATTAGCG 
GGACTGACTG TTCCTTCACG ACTCAAGCGC TCTAACTCTC TCGCCAAACT AGGCAGTCTC 
ACCTTTTCAT CTGAGGACTT GTGCTCGGCC TGCTCCTCTG ACGCAGGGAC CCTTCTCATG 
CTCTCATTGT CCCCAGAACC CGAGCACTCG CTCAGCCCAG AATGGGCAAA ACCAGACAAC 
ATGGAGGTCT TCAGCCAAGG CGAAGCAGGA TGTGAAGAGT CCAAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299661.1
CDS	185..3235
Translation	MHLFGDSCQEVVLKMLPYFADNAFVTQSQIYHILSESFFMVKGAALFLQQGSCPQAQRAHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTNGRQDTEENILLGMDFSNKDSKNCSVGMVLPLWSDTKIHLDGDGGFSVTTAGRTHIFKPVSVQAMWSALQILHKACEVSRRYNYFPGGMALTWMGYYESCISSEQSCINEWNAMQDLETLRPDSPVVFVDKPTERERTECLIKAKLRSIMMCQDLENVTSKEIRNELEQQMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQESGVGHILNVTREIDNFFPGTFCYHNVRVYDDEATDLLAHWNDTYNFIVKAKKNHSKCLVHCKMGVSRSASTVIAYAMKEYGWTLEKAYNFVKQKRSITRPNASFMRQLAEYEGILDASKQRHNRLWRPDSDCDHPECQQAAGPCYLNAETGDHITPDSTTPRVTVCCEPTAHSSPISLDLDPGHPQNYHYYFRRLSDSALESEPSTPVQAQPLVDVDRVYIEVADVEQDALLEDETFEAHEGPPVSHSGEGIGAQTCSRGPEPLEELRLRLEFSTVEEEDEEEAQKEQAEMEALTEEAKRVDGKDSEELLNEKGIDLTSVNHLCNENNNNNHLKMQQILTESAQVKTRLAPGSSLASKPIQSALSEANRRGLAPLPLNSAPCISIQMLSCANLSMTAKCGRTVSDPCLDANAMSLGNNDMPESKSLDQETETQTTLPEAKGEGDFRQEETDLQGSSALPRNERELKGLEKAEASCSPQQQEALIQLQRSGLVRRRKERLEKLSGLFQERARANEQREAGSSKTEDAMNSSSDPADPFHRSFGTRNDFGTESAVPLTNEALLAVLGSGGLTPVSSPHGSTLTRSSSSDSLRSVRGKPGLVRQRTQEIEARMRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSACSSDAGTLLMLSLSPEPEHSLSPEWAKPDNMEVFSQGEAGCEESKS

Target ORF information:

RefSeq Version	XM_009301386.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 1a (ssh1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301386.3

ORF Insert Sequence:

ATGCATCTAT TCGGGGACTC CTGTCAGGAA GTTGTGCTGA AGATGCTGCC GTATTTTGCG 
GATAATGCTT TCGTCACGCA AAGCCAGATC TACCACATTC TGAGCGAGAG TTTCTTCATG 
GTGAAGGGAG CCGCTCTGTT TCTGCAGCAG GGCAGTTGTC CACAGGCTCA GAGAGCTCAT 
CCTCACCACA AACATGCAGG TGACTTGCCT CAGCATCTTC AGGTGATGAT CAACATTCTT 
CGGTCTGAGG ATCGCATCAA ACTGGCGGTG CGTTTGGAGA GCGCGTGGTC AGACCGTGTG 
AGGTACATGG TGGTGGTTTA CACAAATGGC CGACAAGACA CTGAAGAAAA CATCTTATTG 
GGCATGGACT TCAGCAACAA AGACAGTAAG AATTGCTCTG TCGGGATGGT GCTGCCGCTG 
TGGAGTGACA CCAAGATTCA TTTAGACGGT GACGGGGGTT TCAGTGTGAC GACCGCCGGG 
CGTACGCACA TCTTCAAACC GGTGTCAGTC CAGGCCATGT GGTCAGCTTT GCAAATTTTA 
CACAAGGCCT GTGAGGTGTC ACGGCGGTAC AACTATTTCC CTGGTGGGAT GGCACTGACG 
TGGATGGGGT ACTATGAGAG CTGCATCAGC TCTGAACAGA GCTGCATCAA TGAGTGGAAC 
GCCATGCAGG ACCTGGAAAC ACTGAGACCA GACTCGCCTG TAGTCTTTGT CGACAAGCCA 
ACAGAGAGGG AGAGAACAGA ATGTCTAATC AAAGCCAAAC TCAGGAGTAT CATGATGTGC 
CAGGACCTTG AAAATGTCAC GTCCAAAGAG ATTCGTAATG AGTTGGAGCA GCAGATGAAC 
TGTAACCTGA AAGAGTACAA AGAGTTCATA GACAATGAGA TGCTACTTAT TCTTGGCCAG 
ATGGATAAAG CCACTCTCAT CTTTGACCAC CTTTATCTGG GTTCTGAATG GAACGCTTCC 
AATCTAGAGG AACTTCAGGA AAGTGGGGTG GGCCACATCT TAAATGTCAC AAGGGAAATA 
GATAACTTTT TCCCAGGAAC ATTCTGCTAC CACAATGTCA GAGTGTATGA TGATGAGGCC 
ACAGACTTAC TGGCTCACTG GAATGATACC TACAACTTCA TTGTCAAAGC AAAAAAGAAT 
CATTCCAAAT GTTTGGTTCA CTGTAAGATG GGCGTAAGCC GCTCTGCTTC CACGGTGATC 
GCATATGCCA TGAAAGAGTA CGGCTGGACT CTGGAGAAAG CATATAACTT TGTTAAACAG 
AAGCGCAGTA TCACTCGTCC CAATGCCAGC TTCATGAGAC AGCTGGCAGA GTATGAAGGC 
ATACTGGATG CCAGTAAGCA GCGTCATAAC CGTCTGTGGC GCCCTGATTC GGATTGCGAT 
CACCCCGAGT GCCAGCAAGC TGCTGGTCCT TGCTATCTGA ATGCAGAGAC TGGAGATCAC 
ATCACTCCTG ACTCCACAAC CCCCAGGGTC ACTGTCTGCT GTGAGCCTAC AGCCCACTCC 
TCTCCAATTA GTCTAGATCT GGATCCTGGC CATCCACAAA ATTACCACTA CTACTTCCGC 
CGCTTGTCAG ACTCGGCTCT AGAGAGCGAA CCTTCCACCC CTGTCCAAGC ACAGCCTTTG 
GTGGATGTAG ATCGTGTCTA TATTGAGGTT GCCGATGTGG AGCAAGATGC ACTTCTTGAG 
GATGAAACCT TCGAGGCACA TGAGGGCCCT CCTGTTTCCC ACTCAGGAGA GGGTATTGGC 
GCTCAAACAT GTTCTCGAGG ACCGGAGCCA CTAGAAGAAC TACGTCTGCG GTTGGAGTTC 
AGCACAGTGG AAGAAGAGGA TGAAGAAGAG GCTCAGAAAG AGCAAGCTGA GATGGAGGCG 
TTAACAGAGG AAGCAAAGAG AGTAGATGGG AAGGATAGTG AGGAGCTGTT GAATGAAAAG 
GGGATCGATC TGACCAGTGT GAACCATTTG TGCAATGAGA ACAACAACAA CAATCACCTC 
AAAATGCAAC AAATCCTCAC TGAAAGTGCC CAAGTAAAGA CAAGGCTTGC TCCTGGTTCA 
TCGCTTGCTT CCAAGCCCAT CCAAAGTGCT CTTTCGGAGG CTAACAGACG AGGCCTTGCC 
CCTTTACCCC TCAACAGTGC GCCCTGTATC TCCATTCAGA TGCTATCCTG TGCCAACTTG 
TCCATGACAG CCAAGTGTGG CAGAACTGTC TCAGATCCGT GCCTTGATGC CAATGCAATG 
TCCTTAGGGA ATAACGATAT GCCAGAAAGC AAATCTCTTG ACCAGGAAAC TGAAACCCAG 
ACAACTCTGC CTGAGGCCAA AGGAGAAGGA GATTTCAGGC AGGAAGAGAC TGATCTACAG 
GGTTCCTCTG CTCTTCCCCG GAATGAGAGA GAGCTGAAAG GTTTAGAGAA GGCAGAGGCG 
TCTTGTTCTC CTCAACAACA AGAGGCTCTG ATTCAGCTCC AACGCTCCGG TTTAGTTCGA 
CGTCGAAAAG AACGATTGGA AAAACTCTCC GGTCTCTTCC AGGAGCGTGC ACGCGCAAAT 
GAACAACGGG AAGCTGGTTC TAGTAAAACA GAAGATGCAA TGAACAGTTC CAGTGATCCA 
GCTGACCCCT TTCACAGGTC TTTTGGAACA CGGAATGATT TTGGAACTGA ATCGGCAGTG 
CCACTGACCA ATGAGGCTTT GTTGGCAGTC CTCGGATCAG GAGGATTGAC TCCAGTCTCA 
TCTCCACACG GCTCTACGCT CACCCGCAGC TCCAGCAGTG ACAGTCTTCG CAGTGTCCGA 
GGCAAACCAG GCCTTGTGCG TCAGCGTACA CAAGAAATTG AAGCACGAAT GCGATTAGCG 
GGACTGACTG TTCCTTCACG ACTCAAGCGC TCTAACTCTC TCGCCAAACT AGGCAGTCTC 
ACCTTTTCAT CTGAGGACTT GTGCTCGGCC TGCTCCTCTG ACGCAGGGAC CCTTCTCATG 
CTCTCATTGT CCCCAGAACC CGAGCACTCG CTCAGCCCAG AATGGGCAAA ACCAGACAAC 
ATGGAGGTCT TCAGCCAAGG CGAAGCAGGA TGTGAAGAGT CCAAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ssh1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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