LOC101883726 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017351558.1	XP_017207047.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_017351559.1	XP_017207048.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_017351559.2	XP_017207048.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_021467025.1	XP_021322700.1	thrombospondin type-1 domain-containing protein 7A isoform X4
XM_017351560.1	XP_017207049.1	thrombospondin type-1 domain-containing protein 7A isoform X3
XM_017351558.2	XP_017207047.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_021467024.1	XP_021322699.1	thrombospondin type-1 domain-containing protein 7A isoform X3
XM_021467026.1	XP_021322701.1	thrombospondin type-1 domain-containing protein 7A isoform X5
XM_017351560.2	XP_017207049.1	thrombospondin type-1 domain-containing protein 7A isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149454	XM_021467024.1 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa149455	XM_021467025.1 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa149456	XM_021467026.1 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55533	XM_017351558.2 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa55534	XM_017351559.2 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa55535	XM_017351560.2 Latest version!	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
ODa55534	XM_017351559.1	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa55533	XM_017351558.1	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa55535	XM_017351560.1	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

LOC101883726 ( XM_021467024.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322699.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa149454
Clone ID Related Accession (Same CDS sequence)	XM_021467024.1
Accession Version	XM_021467024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGT GACATCAATG 
GTAGTGGCGT GGATTACAGT TATGGAGGGT CTTACAAGGG TTCCCAAGTT TCCTGAACTC 
TATGGAGGAT CCAGCTGCCC GAACACCAGT GCCTTACAGC AGGTGCGTAA CTGTAATGAG 
CACGCTTGTG TGGTGTACCA CTGGCAAACG GGGCCCTGGA GCCTGTGTGT GGAGGACGGC 
AGCTCCTCTA GCCTCAACAG CTCCTCCAGC AACGCAGATA GAGCCTCCTG CACCGCTGGC 
ATCCAGACAC GCAAAGTCAT CTGCGTCAAG GTCAACGTTG GACAGGTGCC ACCCAAAAAG 
TGTCCTGAAG CCCCTCGACC AGAGTCTGTG CAGGTCTGTC AGCTGCCCTG TAAGAGAGAC 
TGTATAGTGA CACCCTACAG TGAATGGACA GAGTGTCCCA CCTCCTGTGA TTCAGGTGCA 
AATGGACAGA AGAATAAGCA GTCTCGCTAT CGAGTGATCA TTCAGAGGCC AGCCAATGGA 
GGTCAGGAGT GTCCAGACAC CCTTTATGAA GAGAAAGACT GCGCAGCTCC ATCTGTCTGT 
CCTGGTTACA GGTGGAAAAC ACACAAATGG CGTAAATGTC AGCTGGTTCC GTGGAGCATC 
CGCAGAGACA GTAAAGGAGC TCAGGAGAAC TGCGGAGCTG GAATCCAGAC TCGAGCGGTG 
TCATGTCGTC AGCTGGACGG TGGTCTGGCG GATGTTCTGC AGTGTTTGCG TTCTGCCAGC 
AGTATGCCTG CGGTGAGCCA GCTCTGCCAG TTGCCATGCC GAGATGACTG CCAGCTCAGC 
GGCTGGTCCC GCTTCTCCCC CTGCTCCTCA GACTGTGTAG GGGTCCGGAC CCGCCGGAGA 
AACCTGCTGG GAAAGAGCAA GAAGAAGGAT AAGTGTAAGA ACAGTCAGAT GTATCCGCTG 
AGCGAGACTC AATACTGTCC CTGTGACAAG TACAACGCGC AGCCGGTGGG AAACTGGTCT 
GACTGTATCC TGCCGGAGGG AATGAAGGAG TGTGGACAGG GCTACCGATA CCAGGCCATG 
GTGTGTTACG ATCAGGACAA CCGGCTGGTG GAGACCTCGC GCTGTAACAG CCACGGGTAT 
ATCGAGGAGG CCTGCATTGT TCCCTGTCCT TCTGACTGTA AACTGAGTGA ATGGTCAAAC 
TGGTCACGCT GCAGTAAATC CTGTGGCAGT GGTGTTAAAG TTCGCTCAAA ATGGCTCAGA 
GAAAAGCCTT ACAACGGAGG AAGACCCTGC CCAAAACTGG ACCACCTGAA CCAGGCACAG 
GTATATGAGG TTGTGCCGTG CGTCAGTGAC TGCAGTCAGT ATGTGTGGGT GTCTGAACCC 
TGGAGTGTGT GGAAAGTGAG TAATGTGGAC CTGAAGGAGA ATTGCGGAGA AGGTGTTCAG 
ACCAGGAGAG TCAGGTGTAT GATGAACACA GTGGACGGTC CTGCTGACGC TGTGGAGGAT 
TATCTGTGTG ACCCAGAGGA GATGCCGCCG GGAACCAGAG AGAGTCGTCT GCCATGCCCA 
GAGGACTGTG TGCTGTCTGA ATGGAGCCGT TGGACCTCCT GTGACCTGCC GTGCACTGGA 
AGAGGAGATC GGGAGAGGAC GGCGTTCGCT CTGCGTTTGC CTAAAGAGGG AGAGGACTGT 
CCTGTCAGCA TGCAGACCGA GCCCTGCCTG CTCAACCGCA ACTGCTTCCA CTACAGCTAC 
AATATCACTG ACTGGAGCAC ATGCCAATTA AGTGCCAACG CTGTGTGTGG AATTGGCCTC 
AAGACGAGGA TGCTGGACTG CGTTAGAAGT GACGGAAAAT CCGTTGACCT GAAGTATTGC 
AAAGAGCTGC GTTTGGAGAA GAAGTGGCAG ATGAACGCAT CCTGTACAGT GGAGTGTCCA 
GTCAACTGTC AGCTCTCTGA ATGGTCTGCA TGGTCGGACT GTTCCCAGAC CTGTGGCCTT 
GAAGGGAAGA TGTGGAGAAA GCGGACGGTG GTTCAGGCGT CTCAGGGTGA CGGTCGGCCG 
TGTCCTTTTC AGATGGAGCA GTGGAAACCC TGTCCCGTTC GGCCCTGCTA CAGATGGGAG 
TACGGGCAGT GGTCTGAGTG CCGCGTCGAG GATGTGGTGT GCGGGGTAGG TGTGCGCTTT 
CGGAATCTGT CCTGTTTTGT GTCTGACGGC TCCAGTGACG GGAAGAGCAG TATTGTGGAG 
GAAGAGCTCT GTGCTGGACT GGAGAAGGCC GTCGATGGAG ATAAGAAGAT TGTCCTGGAG 
GAGACCTGCA CCTTACCCTG CCCTGGTGAC TGTTACCTGA TGGAGTGGTC AGACTGGGGC 
TTGTGTCAGA GTGTGTGTGT GAAAGGTCAG CGTCTGGACT TCACGCCGGT GCAGGTCCGT 
TCAAGGGCTG TCATCGCGCA GGAGCCCGAA AACATCGCTC AGTGTCCTGA TCAGGAGTGG 
CAGTCACGCC CCTGCCGCAA TGGTGAGTGT TATGATTATC AGTGGATGGG AGGACTTCAA 
CTGGTCTGGT GTCAGCGGTC AGATGGAATA AACGTCACAG GCGGATGCCC TTCAGCTCTA 
TTACCAGGGG CCGACCAGTC ATGTGATCCA CTCTGTGACA AACCACAGTC ATTCTGCTCA 
GAGGAGGCCG TGTGTATGTG CGAGGAGGGC TTTACTGAAG TGCTCACCAC CGAGGGAGCG 
CTGGAGCAGT GCGCACCCAT TCCTGTGCTG GAAATCCCCA CTGCTGGAGA CAAGAAGGCT 
GATGTGAAGA CCAGCCGGGC TATCAATCCC ACCCTGCCCA CCACTGTACA GCCTGGACGC 
GTCGGACGCA CGTGGTACTT ACAGCCTTTT GGAGCAGATG GGAAGCTGAA GACGTGGGTA 
TACGGTGTGG CGGCAGGTGT TTTTGTCCTC CTGGTATTTA TAGTGTCGAT GATTTACCTC 
GCATGCAGTT ACTGTGCCAT CCGGCGGTCA CTTGGTACGC CTGATTCTGC CAACGGTACA 
GTAGTGTGGA TGGGCCAAGA TGCCCATGAG CTGACATACA CCTGTCCTGA GTGCCAGCAG 
GAGAGTCAAG GGTCCAGATC CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322699.1
CDS	344..5350
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEVTSMVVAWITVMEGLTRVPKFPELYGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACSYCAIRRSLGTPDSANGTVVWMGQDAHELTYTCPECQQESQGSRSS

Target ORF information:

RefSeq Version	XM_021467024.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467024.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGT GACATCAATG 
GTAGTGGCGT GGATTACAGT TATGGAGGGT CTTACAAGGG TTCCCAAGTT TCCTGAACTC 
TATGGAGGAT CCAGCTGCCC GAACACCAGT GCCTTACAGC AGGTGCGTAA CTGTAATGAG 
CACGCTTGTG TGGTGTACCA CTGGCAAACG GGGCCCTGGA GCCTGTGTGT GGAGGACGGC 
AGCTCCTCTA GCCTCAACAG CTCCTCCAGC AACGCAGATA GAGCCTCCTG CACCGCTGGC 
ATCCAGACAC GCAAAGTCAT CTGCGTCAAG GTCAACGTTG GACAGGTGCC ACCCAAAAAG 
TGTCCTGAAG CCCCTCGACC AGAGTCTGTG CAGGTCTGTC AGCTGCCCTG TAAGAGAGAC 
TGTATAGTGA CACCCTACAG TGAATGGACA GAGTGTCCCA CCTCCTGTGA TTCAGGTGCA 
AATGGACAGA AGAATAAGCA GTCTCGCTAT CGAGTGATCA TTCAGAGGCC AGCCAATGGA 
GGTCAGGAGT GTCCAGACAC CCTTTATGAA GAGAAAGACT GCGCAGCTCC ATCTGTCTGT 
CCTGGTTACA GGTGGAAAAC ACACAAATGG CGTAAATGTC AGCTGGTTCC GTGGAGCATC 
CGCAGAGACA GTAAAGGAGC TCAGGAGAAC TGCGGAGCTG GAATCCAGAC TCGAGCGGTG 
TCATGTCGTC AGCTGGACGG TGGTCTGGCG GATGTTCTGC AGTGTTTGCG TTCTGCCAGC 
AGTATGCCTG CGGTGAGCCA GCTCTGCCAG TTGCCATGCC GAGATGACTG CCAGCTCAGC 
GGCTGGTCCC GCTTCTCCCC CTGCTCCTCA GACTGTGTAG GGGTCCGGAC CCGCCGGAGA 
AACCTGCTGG GAAAGAGCAA GAAGAAGGAT AAGTGTAAGA ACAGTCAGAT GTATCCGCTG 
AGCGAGACTC AATACTGTCC CTGTGACAAG TACAACGCGC AGCCGGTGGG AAACTGGTCT 
GACTGTATCC TGCCGGAGGG AATGAAGGAG TGTGGACAGG GCTACCGATA CCAGGCCATG 
GTGTGTTACG ATCAGGACAA CCGGCTGGTG GAGACCTCGC GCTGTAACAG CCACGGGTAT 
ATCGAGGAGG CCTGCATTGT TCCCTGTCCT TCTGACTGTA AACTGAGTGA ATGGTCAAAC 
TGGTCACGCT GCAGTAAATC CTGTGGCAGT GGTGTTAAAG TTCGCTCAAA ATGGCTCAGA 
GAAAAGCCTT ACAACGGAGG AAGACCCTGC CCAAAACTGG ACCACCTGAA CCAGGCACAG 
GTATATGAGG TTGTGCCGTG CGTCAGTGAC TGCAGTCAGT ATGTGTGGGT GTCTGAACCC 
TGGAGTGTGT GGAAAGTGAG TAATGTGGAC CTGAAGGAGA ATTGCGGAGA AGGTGTTCAG 
ACCAGGAGAG TCAGGTGTAT GATGAACACA GTGGACGGTC CTGCTGACGC TGTGGAGGAT 
TATCTGTGTG ACCCAGAGGA GATGCCGCCG GGAACCAGAG AGAGTCGTCT GCCATGCCCA 
GAGGACTGTG TGCTGTCTGA ATGGAGCCGT TGGACCTCCT GTGACCTGCC GTGCACTGGA 
AGAGGAGATC GGGAGAGGAC GGCGTTCGCT CTGCGTTTGC CTAAAGAGGG AGAGGACTGT 
CCTGTCAGCA TGCAGACCGA GCCCTGCCTG CTCAACCGCA ACTGCTTCCA CTACAGCTAC 
AATATCACTG ACTGGAGCAC ATGCCAATTA AGTGCCAACG CTGTGTGTGG AATTGGCCTC 
AAGACGAGGA TGCTGGACTG CGTTAGAAGT GACGGAAAAT CCGTTGACCT GAAGTATTGC 
AAAGAGCTGC GTTTGGAGAA GAAGTGGCAG ATGAACGCAT CCTGTACAGT GGAGTGTCCA 
GTCAACTGTC AGCTCTCTGA ATGGTCTGCA TGGTCGGACT GTTCCCAGAC CTGTGGCCTT 
GAAGGGAAGA TGTGGAGAAA GCGGACGGTG GTTCAGGCGT CTCAGGGTGA CGGTCGGCCG 
TGTCCTTTTC AGATGGAGCA GTGGAAACCC TGTCCCGTTC GGCCCTGCTA CAGATGGGAG 
TACGGGCAGT GGTCTGAGTG CCGCGTCGAG GATGTGGTGT GCGGGGTAGG TGTGCGCTTT 
CGGAATCTGT CCTGTTTTGT GTCTGACGGC TCCAGTGACG GGAAGAGCAG TATTGTGGAG 
GAAGAGCTCT GTGCTGGACT GGAGAAGGCC GTCGATGGAG ATAAGAAGAT TGTCCTGGAG 
GAGACCTGCA CCTTACCCTG CCCTGGTGAC TGTTACCTGA TGGAGTGGTC AGACTGGGGC 
TTGTGTCAGA GTGTGTGTGT GAAAGGTCAG CGTCTGGACT TCACGCCGGT GCAGGTCCGT 
TCAAGGGCTG TCATCGCGCA GGAGCCCGAA AACATCGCTC AGTGTCCTGA TCAGGAGTGG 
CAGTCACGCC CCTGCCGCAA TGGTGAGTGT TATGATTATC AGTGGATGGG AGGACTTCAA 
CTGGTCTGGT GTCAGCGGTC AGATGGAATA AACGTCACAG GCGGATGCCC TTCAGCTCTA 
TTACCAGGGG CCGACCAGTC ATGTGATCCA CTCTGTGACA AACCACAGTC ATTCTGCTCA 
GAGGAGGCCG TGTGTATGTG CGAGGAGGGC TTTACTGAAG TGCTCACCAC CGAGGGAGCG 
CTGGAGCAGT GCGCACCCAT TCCTGTGCTG GAAATCCCCA CTGCTGGAGA CAAGAAGGCT 
GATGTGAAGA CCAGCCGGGC TATCAATCCC ACCCTGCCCA CCACTGTACA GCCTGGACGC 
GTCGGACGCA CGTGGTACTT ACAGCCTTTT GGAGCAGATG GGAAGCTGAA GACGTGGGTA 
TACGGTGTGG CGGCAGGTGT TTTTGTCCTC CTGGTATTTA TAGTGTCGAT GATTTACCTC 
GCATGCAGTT ACTGTGCCAT CCGGCGGTCA CTTGGTACGC CTGATTCTGC CAACGGTACA 
GTAGTGTGGA TGGGCCAAGA TGCCCATGAG CTGACATACA CCTGTCCTGA GTGCCAGCAG 
GAGAGTCAAG GGTCCAGATC CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_021467025.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322700.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa149455
Clone ID Related Accession (Same CDS sequence)	XM_021467025.1
Accession Version	XM_021467025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGGA 
GGATCCAGCT GCCCGAACAC CAGTGCCTTA CAGCAGGTGC GTAACTGTAA TGAGCACGCT 
TGTGTGGTGT ACCACTGGCA AACGGGGCCC TGGAGCCTGT GTGTGGAGGA CGGCAGCTCC 
TCTAGCCTCA ACAGCTCCTC CAGCAACGCA GATAGAGCCT CCTGCACCGC TGGCATCCAG 
ACACGCAAAG TCATCTGCGT CAAGGTCAAC GTTGGACAGG TGCCACCCAA AAAGTGTCCT 
GAAGCCCCTC GACCAGAGTC TGTGCAGGTC TGTCAGCTGC CCTGTAAGAG AGACTGTATA 
GTGACACCCT ACAGTGAATG GACAGAGTGT CCCACCTCCT GTGATTCAGG TGCAAATGGA 
CAGAAGAATA AGCAGTCTCG CTATCGAGTG ATCATTCAGA GGCCAGCCAA TGGAGGTCAG 
GAGTGTCCAG ACACCCTTTA TGAAGAGAAA GACTGCGCAG CTCCATCTGT CTGTCCTGGT 
TACAGGTGGA AAACACACAA ATGGCGTAAA TGTCAGCTGG TTCCGTGGAG CATCCGCAGA 
GACAGTAAAG GAGCTCAGGA GAACTGCGGA GCTGGAATCC AGACTCGAGC GGTGTCATGT 
CGTCAGCTGG ACGGTGGTCT GGCGGATGTT CTGCAGTGTT TGCGTTCTGC CAGCAGTATG 
CCTGCGGTGA GCCAGCTCTG CCAGTTGCCA TGCCGAGATG ACTGCCAGCT CAGCGGCTGG 
TCCCGCTTCT CCCCCTGCTC CTCAGACTGT GTAGGGGTCC GGACCCGCCG GAGAAACCTG 
CTGGGAAAGA GCAAGAAGAA GGATAAGTGT AAGAACAGTC AGATGTATCC GCTGAGCGAG 
ACTCAATACT GTCCCTGTGA CAAGTACAAC GCGCAGCCGG TGGGAAACTG GTCTGACTGT 
ATCCTGCCGG AGGGAATGAA GGAGTGTGGA CAGGGCTACC GATACCAGGC CATGGTGTGT 
TACGATCAGG ACAACCGGCT GGTGGAGACC TCGCGCTGTA ACAGCCACGG GTATATCGAG 
GAGGCCTGCA TTGTTCCCTG TCCTTCTGAC TGTAAACTGA GTGAATGGTC AAACTGGTCA 
CGCTGCAGTA AATCCTGTGG CAGTGGTGTT AAAGTTCGCT CAAAATGGCT CAGAGAAAAG 
CCTTACAACG GAGGAAGACC CTGCCCAAAA CTGGACCACC TGAACCAGGC ACAGGTATAT 
GAGGTTGTGC CGTGCGTCAG TGACTGCAGT CAGTATGTGT GGGTGTCTGA ACCCTGGAGT 
GTGTGGAAAG TGAGTAATGT GGACCTGAAG GAGAATTGCG GAGAAGGTGT TCAGACCAGG 
AGAGTCAGGT GTATGATGAA CACAGTGGAC GGTCCTGCTG ACGCTGTGGA GGATTATCTG 
TGTGACCCAG AGGAGATGCC GCCGGGAACC AGAGAGAGTC GTCTGCCATG CCCAGAGGAC 
TGTGTGCTGT CTGAATGGAG CCGTTGGACC TCCTGTGACC TGCCGTGCAC TGGAAGAGGA 
GATCGGGAGA GGACGGCGTT CGCTCTGCGT TTGCCTAAAG AGGGAGAGGA CTGTCCTGTC 
AGCATGCAGA CCGAGCCCTG CCTGCTCAAC CGCAACTGCT TCCACTACAG CTACAATATC 
ACTGACTGGA GCACATGCCA ATTAAGTGCC AACGCTGTGT GTGGAATTGG CCTCAAGACG 
AGGATGCTGG ACTGCGTTAG AAGTGACGGA AAATCCGTTG ACCTGAAGTA TTGCAAAGAG 
CTGCGTTTGG AGAAGAAGTG GCAGATGAAC GCATCCTGTA CAGTGGAGTG TCCAGTCAAC 
TGTCAGCTCT CTGAATGGTC TGCATGGTCG GACTGTTCCC AGACCTGTGG CCTTGAAGGG 
AAGATGTGGA GAAAGCGGAC GGTGGTTCAG GCGTCTCAGG GTGACGGTCG GCCGTGTCCT 
TTTCAGATGG AGCAGTGGAA ACCCTGTCCC GTTCGGCCCT GCTACAGATG GGAGTACGGG 
CAGTGGTCTG AGTGCCGCGT CGAGGATGTG GTGTGCGGGG TAGGTGTGCG CTTTCGGAAT 
CTGTCCTGTT TTGTGTCTGA CGGCTCCAGT GACGGGAAGA GCAGTATTGT GGAGGAAGAG 
CTCTGTGCTG GACTGGAGAA GGCCGTCGAT GGAGATAAGA AGATTGTCCT GGAGGAGACC 
TGCACCTTAC CCTGCCCTGG TGACTGTTAC CTGATGGAGT GGTCAGACTG GGGCTTGTGT 
CAGAGTGTGT GTGTGAAAGG TCAGCGTCTG GACTTCACGC CGGTGCAGGT CCGTTCAAGG 
GCTGTCATCG CGCAGGAGCC CGAAAACATC GCTCAGTGTC CTGATCAGGA GTGGCAGTCA 
CGCCCCTGCC GCAATGGTGA GTGTTATGAT TATCAGTGGA TGGGAGGACT TCAACTGGTC 
TGGTGTCAGC GGTCAGATGG AATAAACGTC ACAGGCGGAT GCCCTTCAGC TCTATTACCA 
GGGGCCGACC AGTCATGTGA TCCACTCTGT GACAAACCAC AGTCATTCTG CTCAGAGGAG 
GCCGTGTGTA TGTGCGAGGA GGGCTTTACT GAAGTGCTCA CCACCGAGGG AGCGCTGGAG 
CAGTGCGCAC CCATTCCTGT GCTGGAAATC CCCACTGCTG GAGACAAGAA GGCTGATGTG 
AAGACCAGCC GGGCTATCAA TCCCACCCTG CCCACCACTG TACAGCCTGG ACGCGTCGGA 
CGCACGTGGT ACTTACAGCC TTTTGGAGCA GATGGGAAGC TGAAGACGTG GGTATACGGT 
GTGGCGGCAG GTGTTTTTGT CCTCCTGGTA TTTATAGTGT CGATGATTTA CCTCGCATGC 
AGTTACTGTG CCATCCGGCG GTCACTTGGT ACGCCTGATT CTGCCAACGG TACAGTAGTG 
TGGATGGGCC AAGATGCCCA TGAGCTGACA TACACCTGTC CTGAGTGCCA GCAGGAGAGT 
CAAGGGTCCA GATCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322700.1
CDS	342..5342
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQEGQSSNGQMPSVPPLLSITYEISASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACSYCAIRRSLGTPDSANGTVVWMGQDAHELTYTCPECQQESQGSRSS

Target ORF information:

RefSeq Version	XM_021467025.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467025.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGGA 
GGATCCAGCT GCCCGAACAC CAGTGCCTTA CAGCAGGTGC GTAACTGTAA TGAGCACGCT 
TGTGTGGTGT ACCACTGGCA AACGGGGCCC TGGAGCCTGT GTGTGGAGGA CGGCAGCTCC 
TCTAGCCTCA ACAGCTCCTC CAGCAACGCA GATAGAGCCT CCTGCACCGC TGGCATCCAG 
ACACGCAAAG TCATCTGCGT CAAGGTCAAC GTTGGACAGG TGCCACCCAA AAAGTGTCCT 
GAAGCCCCTC GACCAGAGTC TGTGCAGGTC TGTCAGCTGC CCTGTAAGAG AGACTGTATA 
GTGACACCCT ACAGTGAATG GACAGAGTGT CCCACCTCCT GTGATTCAGG TGCAAATGGA 
CAGAAGAATA AGCAGTCTCG CTATCGAGTG ATCATTCAGA GGCCAGCCAA TGGAGGTCAG 
GAGTGTCCAG ACACCCTTTA TGAAGAGAAA GACTGCGCAG CTCCATCTGT CTGTCCTGGT 
TACAGGTGGA AAACACACAA ATGGCGTAAA TGTCAGCTGG TTCCGTGGAG CATCCGCAGA 
GACAGTAAAG GAGCTCAGGA GAACTGCGGA GCTGGAATCC AGACTCGAGC GGTGTCATGT 
CGTCAGCTGG ACGGTGGTCT GGCGGATGTT CTGCAGTGTT TGCGTTCTGC CAGCAGTATG 
CCTGCGGTGA GCCAGCTCTG CCAGTTGCCA TGCCGAGATG ACTGCCAGCT CAGCGGCTGG 
TCCCGCTTCT CCCCCTGCTC CTCAGACTGT GTAGGGGTCC GGACCCGCCG GAGAAACCTG 
CTGGGAAAGA GCAAGAAGAA GGATAAGTGT AAGAACAGTC AGATGTATCC GCTGAGCGAG 
ACTCAATACT GTCCCTGTGA CAAGTACAAC GCGCAGCCGG TGGGAAACTG GTCTGACTGT 
ATCCTGCCGG AGGGAATGAA GGAGTGTGGA CAGGGCTACC GATACCAGGC CATGGTGTGT 
TACGATCAGG ACAACCGGCT GGTGGAGACC TCGCGCTGTA ACAGCCACGG GTATATCGAG 
GAGGCCTGCA TTGTTCCCTG TCCTTCTGAC TGTAAACTGA GTGAATGGTC AAACTGGTCA 
CGCTGCAGTA AATCCTGTGG CAGTGGTGTT AAAGTTCGCT CAAAATGGCT CAGAGAAAAG 
CCTTACAACG GAGGAAGACC CTGCCCAAAA CTGGACCACC TGAACCAGGC ACAGGTATAT 
GAGGTTGTGC CGTGCGTCAG TGACTGCAGT CAGTATGTGT GGGTGTCTGA ACCCTGGAGT 
GTGTGGAAAG TGAGTAATGT GGACCTGAAG GAGAATTGCG GAGAAGGTGT TCAGACCAGG 
AGAGTCAGGT GTATGATGAA CACAGTGGAC GGTCCTGCTG ACGCTGTGGA GGATTATCTG 
TGTGACCCAG AGGAGATGCC GCCGGGAACC AGAGAGAGTC GTCTGCCATG CCCAGAGGAC 
TGTGTGCTGT CTGAATGGAG CCGTTGGACC TCCTGTGACC TGCCGTGCAC TGGAAGAGGA 
GATCGGGAGA GGACGGCGTT CGCTCTGCGT TTGCCTAAAG AGGGAGAGGA CTGTCCTGTC 
AGCATGCAGA CCGAGCCCTG CCTGCTCAAC CGCAACTGCT TCCACTACAG CTACAATATC 
ACTGACTGGA GCACATGCCA ATTAAGTGCC AACGCTGTGT GTGGAATTGG CCTCAAGACG 
AGGATGCTGG ACTGCGTTAG AAGTGACGGA AAATCCGTTG ACCTGAAGTA TTGCAAAGAG 
CTGCGTTTGG AGAAGAAGTG GCAGATGAAC GCATCCTGTA CAGTGGAGTG TCCAGTCAAC 
TGTCAGCTCT CTGAATGGTC TGCATGGTCG GACTGTTCCC AGACCTGTGG CCTTGAAGGG 
AAGATGTGGA GAAAGCGGAC GGTGGTTCAG GCGTCTCAGG GTGACGGTCG GCCGTGTCCT 
TTTCAGATGG AGCAGTGGAA ACCCTGTCCC GTTCGGCCCT GCTACAGATG GGAGTACGGG 
CAGTGGTCTG AGTGCCGCGT CGAGGATGTG GTGTGCGGGG TAGGTGTGCG CTTTCGGAAT 
CTGTCCTGTT TTGTGTCTGA CGGCTCCAGT GACGGGAAGA GCAGTATTGT GGAGGAAGAG 
CTCTGTGCTG GACTGGAGAA GGCCGTCGAT GGAGATAAGA AGATTGTCCT GGAGGAGACC 
TGCACCTTAC CCTGCCCTGG TGACTGTTAC CTGATGGAGT GGTCAGACTG GGGCTTGTGT 
CAGAGTGTGT GTGTGAAAGG TCAGCGTCTG GACTTCACGC CGGTGCAGGT CCGTTCAAGG 
GCTGTCATCG CGCAGGAGCC CGAAAACATC GCTCAGTGTC CTGATCAGGA GTGGCAGTCA 
CGCCCCTGCC GCAATGGTGA GTGTTATGAT TATCAGTGGA TGGGAGGACT TCAACTGGTC 
TGGTGTCAGC GGTCAGATGG AATAAACGTC ACAGGCGGAT GCCCTTCAGC TCTATTACCA 
GGGGCCGACC AGTCATGTGA TCCACTCTGT GACAAACCAC AGTCATTCTG CTCAGAGGAG 
GCCGTGTGTA TGTGCGAGGA GGGCTTTACT GAAGTGCTCA CCACCGAGGG AGCGCTGGAG 
CAGTGCGCAC CCATTCCTGT GCTGGAAATC CCCACTGCTG GAGACAAGAA GGCTGATGTG 
AAGACCAGCC GGGCTATCAA TCCCACCCTG CCCACCACTG TACAGCCTGG ACGCGTCGGA 
CGCACGTGGT ACTTACAGCC TTTTGGAGCA GATGGGAAGC TGAAGACGTG GGTATACGGT 
GTGGCGGCAG GTGTTTTTGT CCTCCTGGTA TTTATAGTGT CGATGATTTA CCTCGCATGC 
AGTTACTGTG CCATCCGGCG GTCACTTGGT ACGCCTGATT CTGCCAACGG TACAGTAGTG 
TGGATGGGCC AAGATGCCCA TGAGCTGACA TACACCTGTC CTGAGTGCCA GCAGGAGAGT 
CAAGGGTCCA GATCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_021467026.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322701.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X5, mRNA.

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CloneID	ODa149456
Clone ID Related Accession (Same CDS sequence)	XM_021467026.1
Accession Version	XM_021467026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAGTTACTGT 
GCCATCCGGC GGTCACTTGG TACGCCTGAT TCTGCCAACG GTACAGTAGT GTGGATGGGC 
CAAGATGCCC ATGAGCTGAC ATACACCTGT CCTGAGTGCC AGCAGGAGAG TCAAGGGTCC 
AGATCCAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322701.1
CDS	343..5274
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACSYCAIRRSLGTPDSANGTVVWMGQDAHELTYTCPECQQESQGSRSS

Target ORF information:

RefSeq Version	XM_021467026.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467026.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAGTTACTGT 
GCCATCCGGC GGTCACTTGG TACGCCTGAT TCTGCCAACG GTACAGTAGT GTGGATGGGC 
CAAGATGCCC ATGAGCTGAC ATACACCTGT CCTGAGTGCC AGCAGGAGAG TCAAGGGTCC 
AGATCCAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351558.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207047.1 Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

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CloneID	ODa55533
Clone ID Related Accession (Same CDS sequence)	XM_017351558.1 , XM_017351558.2
Accession Version	XM_017351558.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAGTTA CTGTGCCATC CGGCGGTCAC TTGGTACGCC TGATTCTGCC 
AACGGTACAG TAGTGTGGAT GGGCCAAGAT GCCCATGAGC TGACATACAC CTGTCCTGAG 
TGCCAGCAGG AGAGTCAAGG GTCCAGATCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207047.1
CDS	294..5369
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQEGQSSNGQMPSVPPLLSITYEISASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEVTSMVVAWITVMEGLTRVPKFPELYGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACSYCAIRRSLGTPDSANGTVVWMGQDAHELTYTCPECQQESQGSRSS

Target ORF information:

RefSeq Version	XM_017351558.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351558.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAGTTA CTGTGCCATC CGGCGGTCAC TTGGTACGCC TGATTCTGCC 
AACGGTACAG TAGTGTGGAT GGGCCAAGAT GCCCATGAGC TGACATACAC CTGTCCTGAG 
TGCCAGCAGG AGAGTCAAGG GTCCAGATCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207047.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X1, mRNA.

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CloneID	ODa55533
Clone ID Related Accession (Same CDS sequence)	XM_017351558.1 , XM_017351558.2
Accession Version	XM_017351558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAGTTA CTGTGCCATC CGGCGGTCAC TTGGTACGCC TGATTCTGCC 
AACGGTACAG TAGTGTGGAT GGGCCAAGAT GCCCATGAGC TGACATACAC CTGTCCTGAG 
TGCCAGCAGG AGAGTCAAGG GTCCAGATCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207047.1
CDS	343..5418
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQEGQSSNGQMPSVPPLLSITYEISASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEVTSMVVAWITVMEGLTRVPKFPELYGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACSYCAIRRSLGTPDSANGTVVWMGQDAHELTYTCPECQQESQGSRSS

Target ORF information:

RefSeq Version	XM_017351558.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351558.2

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAGTTA CTGTGCCATC CGGCGGTCAC TTGGTACGCC TGATTCTGCC 
AACGGTACAG TAGTGTGGAT GGGCCAAGAT GCCCATGAGC TGACATACAC CTGTCCTGAG 
TGCCAGCAGG AGAGTCAAGG GTCCAGATCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351559.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207048.1 Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X3, mRNA.

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CloneID	ODa55534
Clone ID Related Accession (Same CDS sequence)	XM_017351559.1 , XM_017351559.2
Accession Version	XM_017351559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAAAAA GCCAAAGAAA CCTCAGCGAA GACAAAACAA TAATCGACTG 
AAACCCCTGA CCCTGGCGTA CGATGGCGAT GTGGACATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207048.1
CDS	294..5315
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQEGQSSNGQMPSVPPLLSITYEISASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEVTSMVVAWITVMEGLTRVPKFPELYGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACKKPKKPQRRQNNNRLKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_017351559.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351559.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAAAAA GCCAAAGAAA CCTCAGCGAA GACAAAACAA TAATCGACTG 
AAACCCCTGA CCCTGGCGTA CGATGGCGAT GTGGACATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351559.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207048.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X2, mRNA.

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CloneID	ODa55534
Clone ID Related Accession (Same CDS sequence)	XM_017351559.1 , XM_017351559.2
Accession Version	XM_017351559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAAAAA GCCAAAGAAA CCTCAGCGAA GACAAAACAA TAATCGACTG 
AAACCCCTGA CCCTGGCGTA CGATGGCGAT GTGGACATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207048.1
CDS	342..5363
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQEGQSSNGQMPSVPPLLSITYEISASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEVTSMVVAWITVMEGLTRVPKFPELYGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACKKPKKPQRRQNNNRLKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_017351559.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351559.2

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGAAGGTC AGTCTTCAAA TGGTCAGATG 
CCTAGTGTCC CTCCTCTCCT GTCCATCACC TATGAGATCT CAGCATCTCC ACCGGTGGAT 
GTTCGCCTGT GTAAAGGAGC GACTCCGAGC GCCACACAAC TGTGCCACAT TCAGTGCCCT 
GTGGAGTGTC AGCTGTCCCC ATGGGGCGCC TGGGGGCCCT GCACCTTCGA GAACTGTGAC 
GAACGCAGCG TCAAAAAAGG TTTCAAGTTC AGACGGCGTT CCATCACTAA CTACCCGACG 
GGAGGTGAGG GCAGCTGTGC TCACCTGGTT GAGTCCATCC CGTGTGATGA TCCCAGCTGT 
TTTACCTGGC AGCTGGTCAA GCTGGAGGAG TGCATCCCTG AAGAAGACAT GGACTGTGGA 
GAGGGAACGC AGAATCCGCA AGTCCGATGC ATCAACAACG AAGGTGTAGA GGTGGAGAAG 
GAGATGTGTT CGGATGTGGT TTACCCTTCG CCTGTGCCCT GTGTGGTTCC CTGTCCTAAA 
GACTGTGCTC TGAGCCTCTG GACTGAATGG TCCACCTGTT CTCAGACCTG CTCAAGCAAA 
AACGCTGAAG GCAAGCAGAT GAGGACCAGA GCCATACTGG CCTACAATGC AGGAGAAGTG 
ACATCAATGG TAGTGGCGTG GATTACAGTT ATGGAGGGTC TTACAAGGGT TCCCAAGTTT 
CCTGAACTCT ATGGAGGATC CAGCTGCCCG AACACCAGTG CCTTACAGCA GGTGCGTAAC 
TGTAATGAGC ACGCTTGTGT GGTGTACCAC TGGCAAACGG GGCCCTGGAG CCTGTGTGTG 
GAGGACGGCA GCTCCTCTAG CCTCAACAGC TCCTCCAGCA ACGCAGATAG AGCCTCCTGC 
ACCGCTGGCA TCCAGACACG CAAAGTCATC TGCGTCAAGG TCAACGTTGG ACAGGTGCCA 
CCCAAAAAGT GTCCTGAAGC CCCTCGACCA GAGTCTGTGC AGGTCTGTCA GCTGCCCTGT 
AAGAGAGACT GTATAGTGAC ACCCTACAGT GAATGGACAG AGTGTCCCAC CTCCTGTGAT 
TCAGGTGCAA ATGGACAGAA GAATAAGCAG TCTCGCTATC GAGTGATCAT TCAGAGGCCA 
GCCAATGGAG GTCAGGAGTG TCCAGACACC CTTTATGAAG AGAAAGACTG CGCAGCTCCA 
TCTGTCTGTC CTGGTTACAG GTGGAAAACA CACAAATGGC GTAAATGTCA GCTGGTTCCG 
TGGAGCATCC GCAGAGACAG TAAAGGAGCT CAGGAGAACT GCGGAGCTGG AATCCAGACT 
CGAGCGGTGT CATGTCGTCA GCTGGACGGT GGTCTGGCGG ATGTTCTGCA GTGTTTGCGT 
TCTGCCAGCA GTATGCCTGC GGTGAGCCAG CTCTGCCAGT TGCCATGCCG AGATGACTGC 
CAGCTCAGCG GCTGGTCCCG CTTCTCCCCC TGCTCCTCAG ACTGTGTAGG GGTCCGGACC 
CGCCGGAGAA ACCTGCTGGG AAAGAGCAAG AAGAAGGATA AGTGTAAGAA CAGTCAGATG 
TATCCGCTGA GCGAGACTCA ATACTGTCCC TGTGACAAGT ACAACGCGCA GCCGGTGGGA 
AACTGGTCTG ACTGTATCCT GCCGGAGGGA ATGAAGGAGT GTGGACAGGG CTACCGATAC 
CAGGCCATGG TGTGTTACGA TCAGGACAAC CGGCTGGTGG AGACCTCGCG CTGTAACAGC 
CACGGGTATA TCGAGGAGGC CTGCATTGTT CCCTGTCCTT CTGACTGTAA ACTGAGTGAA 
TGGTCAAACT GGTCACGCTG CAGTAAATCC TGTGGCAGTG GTGTTAAAGT TCGCTCAAAA 
TGGCTCAGAG AAAAGCCTTA CAACGGAGGA AGACCCTGCC CAAAACTGGA CCACCTGAAC 
CAGGCACAGG TATATGAGGT TGTGCCGTGC GTCAGTGACT GCAGTCAGTA TGTGTGGGTG 
TCTGAACCCT GGAGTGTGTG GAAAGTGAGT AATGTGGACC TGAAGGAGAA TTGCGGAGAA 
GGTGTTCAGA CCAGGAGAGT CAGGTGTATG ATGAACACAG TGGACGGTCC TGCTGACGCT 
GTGGAGGATT ATCTGTGTGA CCCAGAGGAG ATGCCGCCGG GAACCAGAGA GAGTCGTCTG 
CCATGCCCAG AGGACTGTGT GCTGTCTGAA TGGAGCCGTT GGACCTCCTG TGACCTGCCG 
TGCACTGGAA GAGGAGATCG GGAGAGGACG GCGTTCGCTC TGCGTTTGCC TAAAGAGGGA 
GAGGACTGTC CTGTCAGCAT GCAGACCGAG CCCTGCCTGC TCAACCGCAA CTGCTTCCAC 
TACAGCTACA ATATCACTGA CTGGAGCACA TGCCAATTAA GTGCCAACGC TGTGTGTGGA 
ATTGGCCTCA AGACGAGGAT GCTGGACTGC GTTAGAAGTG ACGGAAAATC CGTTGACCTG 
AAGTATTGCA AAGAGCTGCG TTTGGAGAAG AAGTGGCAGA TGAACGCATC CTGTACAGTG 
GAGTGTCCAG TCAACTGTCA GCTCTCTGAA TGGTCTGCAT GGTCGGACTG TTCCCAGACC 
TGTGGCCTTG AAGGGAAGAT GTGGAGAAAG CGGACGGTGG TTCAGGCGTC TCAGGGTGAC 
GGTCGGCCGT GTCCTTTTCA GATGGAGCAG TGGAAACCCT GTCCCGTTCG GCCCTGCTAC 
AGATGGGAGT ACGGGCAGTG GTCTGAGTGC CGCGTCGAGG ATGTGGTGTG CGGGGTAGGT 
GTGCGCTTTC GGAATCTGTC CTGTTTTGTG TCTGACGGCT CCAGTGACGG GAAGAGCAGT 
ATTGTGGAGG AAGAGCTCTG TGCTGGACTG GAGAAGGCCG TCGATGGAGA TAAGAAGATT 
GTCCTGGAGG AGACCTGCAC CTTACCCTGC CCTGGTGACT GTTACCTGAT GGAGTGGTCA 
GACTGGGGCT TGTGTCAGAG TGTGTGTGTG AAAGGTCAGC GTCTGGACTT CACGCCGGTG 
CAGGTCCGTT CAAGGGCTGT CATCGCGCAG GAGCCCGAAA ACATCGCTCA GTGTCCTGAT 
CAGGAGTGGC AGTCACGCCC CTGCCGCAAT GGTGAGTGTT ATGATTATCA GTGGATGGGA 
GGACTTCAAC TGGTCTGGTG TCAGCGGTCA GATGGAATAA ACGTCACAGG CGGATGCCCT 
TCAGCTCTAT TACCAGGGGC CGACCAGTCA TGTGATCCAC TCTGTGACAA ACCACAGTCA 
TTCTGCTCAG AGGAGGCCGT GTGTATGTGC GAGGAGGGCT TTACTGAAGT GCTCACCACC 
GAGGGAGCGC TGGAGCAGTG CGCACCCATT CCTGTGCTGG AAATCCCCAC TGCTGGAGAC 
AAGAAGGCTG ATGTGAAGAC CAGCCGGGCT ATCAATCCCA CCCTGCCCAC CACTGTACAG 
CCTGGACGCG TCGGACGCAC GTGGTACTTA CAGCCTTTTG GAGCAGATGG GAAGCTGAAG 
ACGTGGGTAT ACGGTGTGGC GGCAGGTGTT TTTGTCCTCC TGGTATTTAT AGTGTCGATG 
ATTTACCTCG CATGCAAAAA GCCAAAGAAA CCTCAGCGAA GACAAAACAA TAATCGACTG 
AAACCCCTGA CCCTGGCGTA CGATGGCGAT GTGGACATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351560.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207049.1 Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X7, mRNA.

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CloneID	ODa55535
Clone ID Related Accession (Same CDS sequence)	XM_017351560.1 , XM_017351560.2
Accession Version	XM_017351560.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAAAAAGCCA 
AAGAAACCTC AGCGAAGACA AAACAATAAT CGACTGAAAC CCCTGACCCT GGCGTACGAT 
GGCGATGTGG ACATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207049.1
CDS	294..5171
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACKKPKKPQRRQNNNRLKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_017351560.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351560.1

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAAAAAGCCA 
AAGAAACCTC AGCGAAGACA AAACAATAAT CGACTGAAAC CCCTGACCCT GGCGTACGAT 
GGCGATGTGG ACATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101883726 ( XM_017351560.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207049.1 Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X6, mRNA.

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CloneID	ODa55535
Clone ID Related Accession (Same CDS sequence)	XM_017351560.1 , XM_017351560.2
Accession Version	XM_017351560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAAAAAGCCA 
AAGAAACCTC AGCGAAGACA AAACAATAAT CGACTGAAAC CCCTGACCCT GGCGTACGAT 
GGCGATGTGG ACATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207049.1
CDS	341..5218
Translation	MGLGVRGARTAFLCLWRLFYLLCGIWTACSQTTGDGKSLYFWETGPWGRCMGSECGPGGSQSRAVWCAHVEGWTTLHTNCLQSSRPANQQSCFRVCDWHKELYDWRRGAWNQCVPISLRTGRTEACLQGEEGIQTREVYCSLRADGSTADDAICEYFEPKPRQEQACLIPCPKDCVVTEFTPWTTCSKTCGLGLQNRVRSVVAPPLFGGLPCPNLTEFQTCAPRACEGRETVFNLRVGGWSECQSLPVPPVVLPTSTAAPVRAVRQTRRRKGKERKGSRDPEMRELIKSKRNRNRLSRMEDDLWNMEIGFQARQVVCVHRNGSTVPLSQCPHWSLPELYQSCLMPRDCVLSEWSEWSSCSKACSDPKVPQGSRSRSRQLTRLPSGGGLECPQLEESEACEPEGDSVPPCALYTWRSTEWSECRVDALLSQQDRRRANHTGLCGGGVQTREVYCVQANAELLNYINNSKHKQASPPVDVRLCKGATPSATQLCHIQCPVECQLSPWGAWGPCTFENCDERSVKKGFKFRRRSITNYPTGGEGSCAHLVESIPCDDPSCFTWQLVKLEECIPEEDMDCGEGTQNPQVRCINNEGVEVEKEMCSDVVYPSPVPCVVPCPKDCALSLWTEWSTCSQTCSSKNAEGKQMRTRAILAYNAGEGGSSCPNTSALQQVRNCNEHACVVYHWQTGPWSLCVEDGSSSSLNSSSSNADRASCTAGIQTRKVICVKVNVGQVPPKKCPEAPRPESVQVCQLPCKRDCIVTPYSEWTECPTSCDSGANGQKNKQSRYRVIIQRPANGGQECPDTLYEEKDCAAPSVCPGYRWKTHKWRKCQLVPWSIRRDSKGAQENCGAGIQTRAVSCRQLDGGLADVLQCLRSASSMPAVSQLCQLPCRDDCQLSGWSRFSPCSSDCVGVRTRRRNLLGKSKKKDKCKNSQMYPLSETQYCPCDKYNAQPVGNWSDCILPEGMKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIVPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHLNQAQVYEVVPCVSDCSQYVWVSEPWSVWKVSNVDLKENCGEGVQTRRVRCMMNTVDGPADAVEDYLCDPEEMPPGTRESRLPCPEDCVLSEWSRWTSCDLPCTGRGDRERTAFALRLPKEGEDCPVSMQTEPCLLNRNCFHYSYNITDWSTCQLSANAVCGIGLKTRMLDCVRSDGKSVDLKYCKELRLEKKWQMNASCTVECPVNCQLSEWSAWSDCSQTCGLEGKMWRKRTVVQASQGDGRPCPFQMEQWKPCPVRPCYRWEYGQWSECRVEDVVCGVGVRFRNLSCFVSDGSSDGKSSIVEEELCAGLEKAVDGDKKIVLEETCTLPCPGDCYLMEWSDWGLCQSVCVKGQRLDFTPVQVRSRAVIAQEPENIAQCPDQEWQSRPCRNGECYDYQWMGGLQLVWCQRSDGINVTGGCPSALLPGADQSCDPLCDKPQSFCSEEAVCMCEEGFTEVLTTEGALEQCAPIPVLEIPTAGDKKADVKTSRAINPTLPTTVQPGRVGRTWYLQPFGADGKLKTWVYGVAAGVFVLLVFIVSMIYLACKKPKKPQRRQNNNRLKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_017351560.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin type-1 domain-containing protein 7A (LOC101883726), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351560.2

ORF Insert Sequence:

ATGGGACTTG GGGTTAGGGG TGCAAGGACA GCGTTCCTGT GTTTGTGGAG GTTATTTTAT 
CTGCTCTGTG GAATCTGGAC CGCTTGCTCC CAAACCACGG GAGATGGGAA GTCGCTGTAC 
TTCTGGGAAA CAGGTCCGTG GGGCAGGTGT ATGGGCAGCG AGTGCGGTCC CGGCGGCAGT 
CAGAGTCGTG CGGTGTGGTG TGCTCATGTG GAGGGCTGGA CGACCCTCCA CACCAACTGC 
CTTCAGTCCA GCCGACCCGC CAACCAACAG AGCTGCTTCC GCGTCTGCGA CTGGCACAAA 
GAACTGTACG ACTGGCGGCG GGGCGCGTGG AATCAGTGCG TGCCCATATC TCTGCGCACC 
GGCCGCACAG AAGCCTGTCT GCAGGGAGAG GAGGGCATCC AAACACGCGA AGTGTACTGC 
TCGCTCCGAG CGGACGGCTC GACAGCAGAT GATGCCATCT GTGAGTACTT TGAGCCCAAA 
CCCAGACAGG AACAGGCTTG TCTTATTCCG TGCCCTAAGG ACTGTGTGGT GACTGAATTC 
ACCCCGTGGA CCACCTGCTC TAAGACATGC GGGTTGGGTT TGCAGAACCG TGTTCGATCT 
GTAGTGGCGC CGCCGCTTTT CGGTGGACTT CCGTGCCCGA ATTTGACTGA GTTCCAGACG 
TGCGCGCCGA GGGCCTGCGA GGGCCGAGAG ACGGTTTTTA ATCTGAGAGT GGGCGGCTGG 
AGCGAGTGCC AGAGTTTGCC AGTGCCTCCG GTGGTGTTGC CGACCTCCAC GGCCGCCCCC 
GTGCGCGCTG TGCGCCAAAC CCGCCGGCGG AAGGGCAAGG AGCGGAAGGG CAGCAGAGAC 
CCGGAGATGC GGGAGCTCAT CAAGAGCAAG AGAAACCGCA ACCGGCTCAG TCGGATGGAG 
GATGACCTCT GGAATATGGA AATAGGATTT CAGGCCCGAC AAGTGGTTTG CGTTCATCGA 
AATGGAAGTA CAGTGCCGCT CAGTCAGTGT CCTCACTGGA GTCTGCCGGA GCTCTACCAG 
TCCTGCCTGA TGCCCAGAGA CTGTGTGCTG AGTGAGTGGA GCGAGTGGAG CTCCTGCTCG 
AAGGCCTGCT CAGACCCCAA GGTCCCGCAG GGCTCTCGGT CCCGCAGCCG CCAGCTGACC 
CGCCTGCCCA GCGGAGGAGG GTTAGAGTGC CCACAGCTGG AGGAGAGCGA AGCCTGCGAG 
CCGGAGGGAG ACTCAGTGCC ACCCTGCGCC CTGTACACGT GGCGGAGCAC GGAGTGGAGT 
GAGTGTCGTG TGGATGCTCT GCTCAGCCAG CAGGACCGGC GGAGAGCCAA TCACACTGGA 
CTGTGTGGCG GAGGTGTGCA GACCAGAGAG GTTTACTGTG TCCAGGCCAA TGCTGAACTC 
CTCAACTACA TCAATAACAG CAAACACAAA CAAGCATCTC CACCGGTGGA TGTTCGCCTG 
TGTAAAGGAG CGACTCCGAG CGCCACACAA CTGTGCCACA TTCAGTGCCC TGTGGAGTGT 
CAGCTGTCCC CATGGGGCGC CTGGGGGCCC TGCACCTTCG AGAACTGTGA CGAACGCAGC 
GTCAAAAAAG GTTTCAAGTT CAGACGGCGT TCCATCACTA ACTACCCGAC GGGAGGTGAG 
GGCAGCTGTG CTCACCTGGT TGAGTCCATC CCGTGTGATG ATCCCAGCTG TTTTACCTGG 
CAGCTGGTCA AGCTGGAGGA GTGCATCCCT GAAGAAGACA TGGACTGTGG AGAGGGAACG 
CAGAATCCGC AAGTCCGATG CATCAACAAC GAAGGTGTAG AGGTGGAGAA GGAGATGTGT 
TCGGATGTGG TTTACCCTTC GCCTGTGCCC TGTGTGGTTC CCTGTCCTAA AGACTGTGCT 
CTGAGCCTCT GGACTGAATG GTCCACCTGT TCTCAGACCT GCTCAAGCAA AAACGCTGAA 
GGCAAGCAGA TGAGGACCAG AGCCATACTG GCCTACAATG CAGGAGAAGG AGGATCCAGC 
TGCCCGAACA CCAGTGCCTT ACAGCAGGTG CGTAACTGTA ATGAGCACGC TTGTGTGGTG 
TACCACTGGC AAACGGGGCC CTGGAGCCTG TGTGTGGAGG ACGGCAGCTC CTCTAGCCTC 
AACAGCTCCT CCAGCAACGC AGATAGAGCC TCCTGCACCG CTGGCATCCA GACACGCAAA 
GTCATCTGCG TCAAGGTCAA CGTTGGACAG GTGCCACCCA AAAAGTGTCC TGAAGCCCCT 
CGACCAGAGT CTGTGCAGGT CTGTCAGCTG CCCTGTAAGA GAGACTGTAT AGTGACACCC 
TACAGTGAAT GGACAGAGTG TCCCACCTCC TGTGATTCAG GTGCAAATGG ACAGAAGAAT 
AAGCAGTCTC GCTATCGAGT GATCATTCAG AGGCCAGCCA ATGGAGGTCA GGAGTGTCCA 
GACACCCTTT ATGAAGAGAA AGACTGCGCA GCTCCATCTG TCTGTCCTGG TTACAGGTGG 
AAAACACACA AATGGCGTAA ATGTCAGCTG GTTCCGTGGA GCATCCGCAG AGACAGTAAA 
GGAGCTCAGG AGAACTGCGG AGCTGGAATC CAGACTCGAG CGGTGTCATG TCGTCAGCTG 
GACGGTGGTC TGGCGGATGT TCTGCAGTGT TTGCGTTCTG CCAGCAGTAT GCCTGCGGTG 
AGCCAGCTCT GCCAGTTGCC ATGCCGAGAT GACTGCCAGC TCAGCGGCTG GTCCCGCTTC 
TCCCCCTGCT CCTCAGACTG TGTAGGGGTC CGGACCCGCC GGAGAAACCT GCTGGGAAAG 
AGCAAGAAGA AGGATAAGTG TAAGAACAGT CAGATGTATC CGCTGAGCGA GACTCAATAC 
TGTCCCTGTG ACAAGTACAA CGCGCAGCCG GTGGGAAACT GGTCTGACTG TATCCTGCCG 
GAGGGAATGA AGGAGTGTGG ACAGGGCTAC CGATACCAGG CCATGGTGTG TTACGATCAG 
GACAACCGGC TGGTGGAGAC CTCGCGCTGT AACAGCCACG GGTATATCGA GGAGGCCTGC 
ATTGTTCCCT GTCCTTCTGA CTGTAAACTG AGTGAATGGT CAAACTGGTC ACGCTGCAGT 
AAATCCTGTG GCAGTGGTGT TAAAGTTCGC TCAAAATGGC TCAGAGAAAA GCCTTACAAC 
GGAGGAAGAC CCTGCCCAAA ACTGGACCAC CTGAACCAGG CACAGGTATA TGAGGTTGTG 
CCGTGCGTCA GTGACTGCAG TCAGTATGTG TGGGTGTCTG AACCCTGGAG TGTGTGGAAA 
GTGAGTAATG TGGACCTGAA GGAGAATTGC GGAGAAGGTG TTCAGACCAG GAGAGTCAGG 
TGTATGATGA ACACAGTGGA CGGTCCTGCT GACGCTGTGG AGGATTATCT GTGTGACCCA 
GAGGAGATGC CGCCGGGAAC CAGAGAGAGT CGTCTGCCAT GCCCAGAGGA CTGTGTGCTG 
TCTGAATGGA GCCGTTGGAC CTCCTGTGAC CTGCCGTGCA CTGGAAGAGG AGATCGGGAG 
AGGACGGCGT TCGCTCTGCG TTTGCCTAAA GAGGGAGAGG ACTGTCCTGT CAGCATGCAG 
ACCGAGCCCT GCCTGCTCAA CCGCAACTGC TTCCACTACA GCTACAATAT CACTGACTGG 
AGCACATGCC AATTAAGTGC CAACGCTGTG TGTGGAATTG GCCTCAAGAC GAGGATGCTG 
GACTGCGTTA GAAGTGACGG AAAATCCGTT GACCTGAAGT ATTGCAAAGA GCTGCGTTTG 
GAGAAGAAGT GGCAGATGAA CGCATCCTGT ACAGTGGAGT GTCCAGTCAA CTGTCAGCTC 
TCTGAATGGT CTGCATGGTC GGACTGTTCC CAGACCTGTG GCCTTGAAGG GAAGATGTGG 
AGAAAGCGGA CGGTGGTTCA GGCGTCTCAG GGTGACGGTC GGCCGTGTCC TTTTCAGATG 
GAGCAGTGGA AACCCTGTCC CGTTCGGCCC TGCTACAGAT GGGAGTACGG GCAGTGGTCT 
GAGTGCCGCG TCGAGGATGT GGTGTGCGGG GTAGGTGTGC GCTTTCGGAA TCTGTCCTGT 
TTTGTGTCTG ACGGCTCCAG TGACGGGAAG AGCAGTATTG TGGAGGAAGA GCTCTGTGCT 
GGACTGGAGA AGGCCGTCGA TGGAGATAAG AAGATTGTCC TGGAGGAGAC CTGCACCTTA 
CCCTGCCCTG GTGACTGTTA CCTGATGGAG TGGTCAGACT GGGGCTTGTG TCAGAGTGTG 
TGTGTGAAAG GTCAGCGTCT GGACTTCACG CCGGTGCAGG TCCGTTCAAG GGCTGTCATC 
GCGCAGGAGC CCGAAAACAT CGCTCAGTGT CCTGATCAGG AGTGGCAGTC ACGCCCCTGC 
CGCAATGGTG AGTGTTATGA TTATCAGTGG ATGGGAGGAC TTCAACTGGT CTGGTGTCAG 
CGGTCAGATG GAATAAACGT CACAGGCGGA TGCCCTTCAG CTCTATTACC AGGGGCCGAC 
CAGTCATGTG ATCCACTCTG TGACAAACCA CAGTCATTCT GCTCAGAGGA GGCCGTGTGT 
ATGTGCGAGG AGGGCTTTAC TGAAGTGCTC ACCACCGAGG GAGCGCTGGA GCAGTGCGCA 
CCCATTCCTG TGCTGGAAAT CCCCACTGCT GGAGACAAGA AGGCTGATGT GAAGACCAGC 
CGGGCTATCA ATCCCACCCT GCCCACCACT GTACAGCCTG GACGCGTCGG ACGCACGTGG 
TACTTACAGC CTTTTGGAGC AGATGGGAAG CTGAAGACGT GGGTATACGG TGTGGCGGCA 
GGTGTTTTTG TCCTCCTGGT ATTTATAGTG TCGATGATTT ACCTCGCATG CAAAAAGCCA 
AAGAAACCTC AGCGAAGACA AAACAATAAT CGACTGAAAC CCCTGACCCT GGCGTACGAT 
GGCGATGTGG ACATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC101883726
Entrez Gene ID	101883726
Full Name	thrombospondin type-1 domain-containing protein 7A
Synonyms	thsd7a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

LOC101883726 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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