NPC1L1 cDNA ORF clone, Danio rerio(Zebrafish)

The following NPC1L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa143814 XM_021478480.1
Latest version!
Danio rerio NPC1-like 1 (npc1l1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa60178 XM_009304174.2
Latest version!
Danio rerio NPC1-like 1 (npc1l1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa143814
    Clone ID Related Accession (Same CDS sequence) XM_021478480.1
    Accession Version XM_021478480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Niemann-Pick C1-like protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) and transcript sequence (GFIL01016470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 497 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCATTTCC AAAGGAAGAA CTCTGCGTGG TCCATTACTT TGATTTCACT TGCTGCTTGT 
    TTGGTTCTCA CAGGTGCTCA GCATGAACCA GGCTTCTGCG CAATGTATGA GGACTGTGGG
    TTAAATCCCG CAGTGGAAGG GGCTCTTATC CCCCCAAGAG TTCCCTGTAA GGACTACAGA
    AGAGCAGTGA ATGTGACAGG AGACCACTAT GAGCTTCTGA AATCTGTATG TCCAATGCTG
    GCCAATGGCG ATGGCCAAAC ATTGGCCTGC TGCTCCATCA GGCAACTCAC AGCTCTGCAA
    AACAGCCTGT CGCTCTCCAA AGCCGTTCTG ATCCGCTGCC CGTCCTGTGC CGAGAACTTT
    GCGTATCTCC ATTGCGCCAC GACCTGCAGT CCTGACCAAA GTCAGATATT GAAGATCACC
    AAAACGGCCA ACATCACTCA ACCCACAGGC ATTGTAAAAG AAGCCGTGGT GGGTTACGCA
    GCGTATATAT CCACTAACTT CTCTGATGCC TCGTTTGACT CCTGCAAAAA TGTACGAATT
    CCATCCACCG GAGGGTTTGC CATTGCCACA ATGTGCGGCC GTTATGGTGC AGCACTCTGT
    ACGCCACAGC GCTGGTATGA TTTTCAGGGC GACTCCAGCA ATGGATTGGC TCCGCTGGAT
    ATTGACTTCA AGCTTTTACA AGACAGCGAG ACCTCTGAAG TCCCAGCAGG AGCATTTGCG
    TTTGCAGGAC AAGCGTTAAA ATGCAATGAA GTCACACCTT CCGGTGGCGA GGCCTGTTCC
    TGTCAGGACT GCCTACAATC TTGTCCGGTG GTGCCAGAAC CACCGCCTCT GCCGAAACCC
    TTCATGATTG GTAACCTTAA TGGGGTGCTT GTCATCTGCC TCACAGTCTT CTCTTGCTTC
    CTTTTTCTTT TCCTGTGTTA CGTGATTACC GAGTGCACGA CTTACTACAT GAAGTCGAGG
    AAGGGTCGGA AGGGTAAGAG CACCAAGGAT CAAAACGCAA ATGAGATCAA ATTTAAAATC
    AGCCCTAAAG ATGTTTCGTG CTCTGACAAA GCCAGTCTTG CCACTCAGGA ATTCCTAGGG
    TCTCTTTTCC AGTCCTGGGG GACCTTAATG GCACGGTATC CCTACATAGT GCTTCCGGTC
    TGCCTTGTGA TTGTGCTGGT GTTTGCGGTG GGAATAAAGG ACATAGAACT GACCACAGAT
    CCCGTCCAGC TCTGGTCAGC ACCTGAAAGT CGCGCCATGC GGGAGAAGGC CTTCCACGAT
    GCTCATTTCG ATCCGTTTTA CCGCACCAAT CAGCTGATCT TGACGGCTCC AGGCCGGCCC
    AGCCACTTCT ATGACTCCTT ACTCTTTGGC AAGCAGAACT TCAGTGGCAT CATCTCCAAA
    GATTTGATCA TAGAGCTGTT AAAGCTTCAG CAGAGCATCC AAGCAATTGA GTTCTGGTCA
    GATGAACTAA ACAGTACCGC CAGCCTTAAG GATGTTTGCT ATGCGCCACT CAACCCAGGC
    AACCCCTCGT TAACCGACTG CGCTGTCAAC AGTCTGCCGC AGTACTTCCA AAATAGCATC
    GACAACCTGA ATGCCAAAGT GAACATGACA GAGCTTGGTG TGACTAAGGA AGTGGACTGG
    CGGGATCATT TCATCTACTG TGTTAACTCT CCACTATCAT TTAAAGACAT CACAGCTCTC
    GGCATGAGCT GTATGTCTGA CTATGGAGGC CCTGTGTTCC CCTTCCTGGC TGTCGGAGGA
    TATGATAATG AACAATACAC CACTGCTGAG GCCCTGATCC TCACTTTTTC CCTCAACAAT
    TATGCAAGAA CAGACGTTAA ATTTAAGGTG GTTGAAGAGT GGGAAAACCG GTTTCTTAAA
    ATCGTCCAGG AGTATCAGAA AAACTCTACA ACAAACTTCA CATTTGCTTA CATGGCTGAG
    AGGTCTTTGG AAGATGAGAT TAACAGAACG ACAGCGGAGG ATATCCCCAT ATTCATGATA
    AGCTATGCTG TCATCTTCCT CTACATCGCA GTGGCTCTTG GGGAATATTC ATCCTGCAAG
    CGTATCCTGG TAGACTCGAA ATTCCTGGTA GGCCTGGGTG GGATTCTGGT AGTCGGCTGC
    TCTGTCATAG CCTCAATGGG TTTCTATGCT TGGATTGGTG TGGCGTCCTC GCTGGTCATT
    CTGCAGGTGG TGCCCTTCCT TGTACTGGCC GTGGGAGCTG ACAACATCTT CATTTTTGTC
    CTGGAGTATC AGAGAGACCT GCGCAGACCA GGAGAGCAGC GGGAGGAACA GATCGGCAGA
    GTTCTCGGAA ACGTGGCTCC CAGCATGCTG CTCTGCAGTT TGTCTGAATC TGTTTGTTTC
    TTCCTGGGAG CATTGAGCAC CATGCCAGCA GTGAAGTCTT TCGCTCTGTA CGCTGCATTG
    GCTGTGCTGA TGGACTTTGT TCTCCAGATG ACTGCCTTTG TGGCTCTGCT TTCTCTGGAT
    GCTCGCCGTC AGGACTCAAA CCGCTGTGAG ATAGCCTGCT GCGTGACAGT GGACACTCCA
    CGACCGCAAA AACCCAACGA GGGCATCCTG CTGCCGCTCA TGAGGAAATA CTACGCTCCG
    TTCCTACTCA ACTCTTTCAG CAGAGTAGTA GTGATGGTGT TGTTCCTGGT GATGTTCTTT
    AGCTCTGTGT TCCTCATGTT TCATGTCAAA GTGGGCTTGA ATCAGGAACT GGCCATGCCA
    ACTGACTCCT ACATGCTGGA CTACTTTGCA TACCTGTTTA AGTATTTTGA GGTTGGTGTG
    CCCACGTATT TCATCACGAC CAAAGGGTTC AACTTCAACA GCGAGGAGGG CATTAATGCA
    GTGTGTTCCA GTGTGGGATG TGACCAGTTC TCTATGACCC AGAAGATCCG CTACGCTACT
    GAATATCCTG AGCGATCTTA TCTGGCTATC CCAGCATCCT CTTGGGTGGA TGACTATATT
    GACTGGCTGA ACCCTGGATC CAGATGCTGT CGCCTTTACA CATTTGGTCC AAACAAAGAT
    GAATTTTGTC CTGCCAATGA GACCGGTGTC TGTCTCAAGA AGTGTATGGG CAAACCTGAA
    AATGGTGTAC TGCGGCCTGA TGTTTCAGAA TTCAACAGAT TTCTGCCAGA TTTTCTGAGC
    AACAGACCTG ACCTGCAGTG TCCAAAAGGT GGTTTGGGAG CCTATGATAA AGCTGTGATT
    AGAGACAATG AAACTGGGGA AATAATAGCT ACTCGGTTCA TGGCCTATCA CACTCCTCTG
    GTGAATTCGC AGGAGTACAC CGGAGCGCTG CAGAAAGCCA GAGAACTGGC CCATAACATC
    ACCATGGCCA TGAGGAAACT CAACGACACC TCTCCAGATT TTGAGGTCTT CCCTTACACT
    GTGACGTATG TGTATTACGA GCAGTACCTG AACATCGTGA ATGAGGGTTT GTTCAACATC
    GCCGTGTGTT TGCTGCCCAC ATTCGTGGTG TGTTGTATTC TGCTCGGCAT GGACCTGAGA
    TCCGGCTTTC TGAACCTGCT CACTATAGTG ATGATCACTG TGGACACAGT TGGCGTCATG
    ACCCTTTGGG GCATCGACTT CAACGCCGTG TCACTTATTA ACCTGGTGAC GGCTGTTGGT
    ATATCAGTGG AGTTTGTGTC TCATACAACA CGCTCATTTG CTTTAAGCGT TCGCCCCACA
    AAAGTGGAGA GAGCAAAGGA AGCCACAGCT AACATGGGCA GTGCGGTGTT TGCTGGTGTT
    GCGATGACCA ATCTGCCCGG AATTGTTGTT TTGGCGTTTG CTAAAGCTCA GCTCATCCAG
    ATCTTCTTCT TCAGACTCAA TCTGGTCATA ACTCTTCTCG GCATGGCCCA TGGACTCATA
    TTTCTGCCTG TGCTGCTCAG TTACTTTGGT CCTGGAGTAA ACAACGCACT TCTGCTGCAG
    ATTCAAGAAG AACAAGAAAA AGAGATGGAG CTCAATAGTA AAGCTACATA TGACAACGTT
    TGCTTTGAAA ACCATGAAAA ATCTAACGGC GAAATCCACG TAGAGCCTGC AAGTTTGCCT
    GAAAACCATG AAAAGTCCAA CGGCGTAATC CCTGCAGAGC CTTCAAGTTT CCCTGTAGAG
    CCTTCGAGCC CTCCAGAAGA GCCGTCAGTC CTAATAATGG CAGTGATTAT GCCTGAAACC
    ACCCAAATGC CCACCGAATG GATATCTCCA AGTTGTAGCC CTAAAACTTT CTTTGAGTTT
    ATTTACCATA TCATTATACT GAACTGGAAG TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334155.1
    CDS114..4349
    Translation

    Target ORF information:

    RefSeq Version XM_021478480.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NPC1-like 1 (npc1l1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCATTTCC AAAGGAAGAA CTCTGCGTGG TCCATTACTT TGATTTCACT TGCTGCTTGT 
    TTGGTTCTCA CAGGTGCTCA GCATGAACCA GGCTTCTGCG CAATGTATGA GGACTGTGGG
    TTAAATCCCG CAGTGGAAGG GGCTCTTATC CCCCCAAGAG TTCCCTGTAA GGACTACAGA
    AGAGCAGTGA ATGTGACAGG AGACCACTAT GAGCTTCTGA AATCTGTATG TCCAATGCTG
    GCCAATGGCG ATGGCCAAAC ATTGGCCTGC TGCTCCATCA GGCAACTCAC AGCTCTGCAA
    AACAGCCTGT CGCTCTCCAA AGCCGTTCTG ATCCGCTGCC CGTCCTGTGC CGAGAACTTT
    GCGTATCTCC ATTGCGCCAC GACCTGCAGT CCTGACCAAA GTCAGATATT GAAGATCACC
    AAAACGGCCA ACATCACTCA ACCCACAGGC ATTGTAAAAG AAGCCGTGGT GGGTTACGCA
    GCGTATATAT CCACTAACTT CTCTGATGCC TCGTTTGACT CCTGCAAAAA TGTACGAATT
    CCATCCACCG GAGGGTTTGC CATTGCCACA ATGTGCGGCC GTTATGGTGC AGCACTCTGT
    ACGCCACAGC GCTGGTATGA TTTTCAGGGC GACTCCAGCA ATGGATTGGC TCCGCTGGAT
    ATTGACTTCA AGCTTTTACA AGACAGCGAG ACCTCTGAAG TCCCAGCAGG AGCATTTGCG
    TTTGCAGGAC AAGCGTTAAA ATGCAATGAA GTCACACCTT CCGGTGGCGA GGCCTGTTCC
    TGTCAGGACT GCCTACAATC TTGTCCGGTG GTGCCAGAAC CACCGCCTCT GCCGAAACCC
    TTCATGATTG GTAACCTTAA TGGGGTGCTT GTCATCTGCC TCACAGTCTT CTCTTGCTTC
    CTTTTTCTTT TCCTGTGTTA CGTGATTACC GAGTGCACGA CTTACTACAT GAAGTCGAGG
    AAGGGTCGGA AGGGTAAGAG CACCAAGGAT CAAAACGCAA ATGAGATCAA ATTTAAAATC
    AGCCCTAAAG ATGTTTCGTG CTCTGACAAA GCCAGTCTTG CCACTCAGGA ATTCCTAGGG
    TCTCTTTTCC AGTCCTGGGG GACCTTAATG GCACGGTATC CCTACATAGT GCTTCCGGTC
    TGCCTTGTGA TTGTGCTGGT GTTTGCGGTG GGAATAAAGG ACATAGAACT GACCACAGAT
    CCCGTCCAGC TCTGGTCAGC ACCTGAAAGT CGCGCCATGC GGGAGAAGGC CTTCCACGAT
    GCTCATTTCG ATCCGTTTTA CCGCACCAAT CAGCTGATCT TGACGGCTCC AGGCCGGCCC
    AGCCACTTCT ATGACTCCTT ACTCTTTGGC AAGCAGAACT TCAGTGGCAT CATCTCCAAA
    GATTTGATCA TAGAGCTGTT AAAGCTTCAG CAGAGCATCC AAGCAATTGA GTTCTGGTCA
    GATGAACTAA ACAGTACCGC CAGCCTTAAG GATGTTTGCT ATGCGCCACT CAACCCAGGC
    AACCCCTCGT TAACCGACTG CGCTGTCAAC AGTCTGCCGC AGTACTTCCA AAATAGCATC
    GACAACCTGA ATGCCAAAGT GAACATGACA GAGCTTGGTG TGACTAAGGA AGTGGACTGG
    CGGGATCATT TCATCTACTG TGTTAACTCT CCACTATCAT TTAAAGACAT CACAGCTCTC
    GGCATGAGCT GTATGTCTGA CTATGGAGGC CCTGTGTTCC CCTTCCTGGC TGTCGGAGGA
    TATGATAATG AACAATACAC CACTGCTGAG GCCCTGATCC TCACTTTTTC CCTCAACAAT
    TATGCAAGAA CAGACGTTAA ATTTAAGGTG GTTGAAGAGT GGGAAAACCG GTTTCTTAAA
    ATCGTCCAGG AGTATCAGAA AAACTCTACA ACAAACTTCA CATTTGCTTA CATGGCTGAG
    AGGTCTTTGG AAGATGAGAT TAACAGAACG ACAGCGGAGG ATATCCCCAT ATTCATGATA
    AGCTATGCTG TCATCTTCCT CTACATCGCA GTGGCTCTTG GGGAATATTC ATCCTGCAAG
    CGTATCCTGG TAGACTCGAA ATTCCTGGTA GGCCTGGGTG GGATTCTGGT AGTCGGCTGC
    TCTGTCATAG CCTCAATGGG TTTCTATGCT TGGATTGGTG TGGCGTCCTC GCTGGTCATT
    CTGCAGGTGG TGCCCTTCCT TGTACTGGCC GTGGGAGCTG ACAACATCTT CATTTTTGTC
    CTGGAGTATC AGAGAGACCT GCGCAGACCA GGAGAGCAGC GGGAGGAACA GATCGGCAGA
    GTTCTCGGAA ACGTGGCTCC CAGCATGCTG CTCTGCAGTT TGTCTGAATC TGTTTGTTTC
    TTCCTGGGAG CATTGAGCAC CATGCCAGCA GTGAAGTCTT TCGCTCTGTA CGCTGCATTG
    GCTGTGCTGA TGGACTTTGT TCTCCAGATG ACTGCCTTTG TGGCTCTGCT TTCTCTGGAT
    GCTCGCCGTC AGGACTCAAA CCGCTGTGAG ATAGCCTGCT GCGTGACAGT GGACACTCCA
    CGACCGCAAA AACCCAACGA GGGCATCCTG CTGCCGCTCA TGAGGAAATA CTACGCTCCG
    TTCCTACTCA ACTCTTTCAG CAGAGTAGTA GTGATGGTGT TGTTCCTGGT GATGTTCTTT
    AGCTCTGTGT TCCTCATGTT TCATGTCAAA GTGGGCTTGA ATCAGGAACT GGCCATGCCA
    ACTGACTCCT ACATGCTGGA CTACTTTGCA TACCTGTTTA AGTATTTTGA GGTTGGTGTG
    CCCACGTATT TCATCACGAC CAAAGGGTTC AACTTCAACA GCGAGGAGGG CATTAATGCA
    GTGTGTTCCA GTGTGGGATG TGACCAGTTC TCTATGACCC AGAAGATCCG CTACGCTACT
    GAATATCCTG AGCGATCTTA TCTGGCTATC CCAGCATCCT CTTGGGTGGA TGACTATATT
    GACTGGCTGA ACCCTGGATC CAGATGCTGT CGCCTTTACA CATTTGGTCC AAACAAAGAT
    GAATTTTGTC CTGCCAATGA GACCGGTGTC TGTCTCAAGA AGTGTATGGG CAAACCTGAA
    AATGGTGTAC TGCGGCCTGA TGTTTCAGAA TTCAACAGAT TTCTGCCAGA TTTTCTGAGC
    AACAGACCTG ACCTGCAGTG TCCAAAAGGT GGTTTGGGAG CCTATGATAA AGCTGTGATT
    AGAGACAATG AAACTGGGGA AATAATAGCT ACTCGGTTCA TGGCCTATCA CACTCCTCTG
    GTGAATTCGC AGGAGTACAC CGGAGCGCTG CAGAAAGCCA GAGAACTGGC CCATAACATC
    ACCATGGCCA TGAGGAAACT CAACGACACC TCTCCAGATT TTGAGGTCTT CCCTTACACT
    GTGACGTATG TGTATTACGA GCAGTACCTG AACATCGTGA ATGAGGGTTT GTTCAACATC
    GCCGTGTGTT TGCTGCCCAC ATTCGTGGTG TGTTGTATTC TGCTCGGCAT GGACCTGAGA
    TCCGGCTTTC TGAACCTGCT CACTATAGTG ATGATCACTG TGGACACAGT TGGCGTCATG
    ACCCTTTGGG GCATCGACTT CAACGCCGTG TCACTTATTA ACCTGGTGAC GGCTGTTGGT
    ATATCAGTGG AGTTTGTGTC TCATACAACA CGCTCATTTG CTTTAAGCGT TCGCCCCACA
    AAAGTGGAGA GAGCAAAGGA AGCCACAGCT AACATGGGCA GTGCGGTGTT TGCTGGTGTT
    GCGATGACCA ATCTGCCCGG AATTGTTGTT TTGGCGTTTG CTAAAGCTCA GCTCATCCAG
    ATCTTCTTCT TCAGACTCAA TCTGGTCATA ACTCTTCTCG GCATGGCCCA TGGACTCATA
    TTTCTGCCTG TGCTGCTCAG TTACTTTGGT CCTGGAGTAA ACAACGCACT TCTGCTGCAG
    ATTCAAGAAG AACAAGAAAA AGAGATGGAG CTCAATAGTA AAGCTACATA TGACAACGTT
    TGCTTTGAAA ACCATGAAAA ATCTAACGGC GAAATCCACG TAGAGCCTGC AAGTTTGCCT
    GAAAACCATG AAAAGTCCAA CGGCGTAATC CCTGCAGAGC CTTCAAGTTT CCCTGTAGAG
    CCTTCGAGCC CTCCAGAAGA GCCGTCAGTC CTAATAATGG CAGTGATTAT GCCTGAAACC
    ACCCAAATGC CCACCGAATG GATATCTCCA AGTTGTAGCC CTAAAACTTT CTTTGAGTTT
    ATTTACCATA TCATTATACT GAACTGGAAG TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60178
    Clone ID Related Accession (Same CDS sequence) XM_009304174.2
    Accession Version XM_009304174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Niemann-Pick C1-like protein 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCATTTCC AAAGGAAGAA CTCTGCGTGG TCCATTACTT TGATTTCACT TGCTGCTTGT 
    TTGGTTCTCA CAGGTGCTCA GCATGAACCA GGCTTCTGCG CAATGTATGA GGACTGTGGG
    TTAAATCCCG CAGTGGAAGG GGCTCTTATC CCCCCAAGAG TTCCCTGTAA GGACTACAGA
    AGAGCAGTGA ATGTGACAGG AGACCACTAT GAGCTTCTGA AATCTGTATG TCCAATGCTG
    GCCAATGGCG ATGGCCAAAC ATTGGCCTGC TGCTCCATCA GGCAACTCAC AGCTCTGCAA
    AACAGCCTGT CGCTCTCCAA AGCCGTTCTG ATCCGCTGCC CGTCCTGTGC CGAGAACTTT
    GCGTATCTCC ATTGCGCCAC GACCTGCAGT CCTGACCAAA GTCAGATATT GAAGATCACC
    AAAACGGCCA ACATCACTCA ACCCACAGGC ATTGTAAAAG AAGCCGTGGT GGGTTACGCA
    GCGTATATAT CCACTAACTT CTCTGATGCC TCGTTTGACT CCTGCAAAAA TGTACGAATT
    CCATCCACCG GAGGGTTTGC CATTGCCACA ATGTGCGGCC GTTATGGTGC AGCACTCTGT
    ACGCCACAGC GCTGGTATGA TTTTCAGGGC GACTCCAGCA ATGGATTGGC TCCGCTGGAT
    ATTGACTTCA AGCTTTTACA AGACAGCGAG ACCTCTGAAG TCCCAGCAGG AGCATTTGCG
    TTTGCAGGAC AAGCGTTAAA ATGCAATGAA GTCACACCTT CCGGTGGCGA GGCCTGTTCC
    TGTCAGGACT GCCTACAATC TTGTCCGGTG GTGCCAGAAC CACCGCCTCT GCCGAAACCC
    TTCATGATTG GTAACCTTAA TGGGGTGCTT GTCATCTGCC TCACAGTCTT CTCTTGCTTC
    CTTTTTCTTT TCCTGTGTTA CGTGATTACC GAGTGCACGA CTTACTACAT GAAGTCGAGG
    AAGGGTCGGA AGGGTAAGAG CACCAAGGAT CAAAACGCAA ATGAGATCAA ATTTAAAATC
    AGCCCTAAAG ATGTTTCGTG CTCTGACAAA GCCAGTCTTG CCACTCAGGA ATTCCTAGGG
    TCTCTTTTCC AGTCCTGGGG GACCTTAATG GCACGGTATC CCTACATAGT GCTTCCGGTC
    TGCCTTGTGA TTGTGCTGGT GTTTGCGGTG GGAATAAAGG ACATAGAACT GACCACAGAT
    CCCGTCCAGC TCTGGTCAGC ACCTGAAAGT CGCGCCATGC GGGAGAAGGC CTTCCACGAT
    GCTCATTTCG ATCCGTTTTA CCGCACCAAT CAGCTGATCT TGACGGCTCC AGGCCGGCCC
    AGCCACTTCT ATGACTCCTT ACTCTTTGGC AAGCAGAACT TCAGTGGCAT CATCTCCAAA
    GATTTGATCA TAGAGCTGTT AAAGCTTCAG CAGAGCATCC AAGCAATTGA GTTCTGGTCA
    GATGAACTAA ACAGTACCGC CAGCCTTAAG GATGTTTGCT ATGCGCCACT CAACCCAGGC
    AACCCCTCGT TAACCGACTG CGCTGTCAAC AGTCTGCCGC AGTACTTCCA AAATAGCATC
    GACAACCTGA ATGCCAAAGT GAACATGACA GAGCTTGGTG TGACTAAGGA AGTGGACTGG
    CGGGATCATT TCATCTACTG TGTTAACTCT CCACTATCAT TTAAAGACAT CACAGCTCTC
    GGCATGAGCT GTATGTCTGA CTATGGAGGC CCTGTGTTCC CCTTCCTGGC TGTCGGAGGA
    TATGATAATG AACAATACAC CACTGCTGAG GCCCTGATCC TCACTTTTTC CCTCAACAAT
    TATGCAAGAA CAGACGTTAA ATTTAAGGTG GTTGAAGAGT GGGAAAACCG GTTTCTTAAA
    ATCGTCCAGG AGTATCAGAA AAACTCTACA ACAAACTTCA CATTTGCTTA CATGGCTGAG
    AGGTCTTTGG AAGATGAGAT TAACAGAACG ACAGCGGAGG ATATCCCCAT ATTCATGATA
    AGCTATGCTG TCATCTTCCT CTACATCGCA GTGGCTCTTG GGGAATATTC ATCCTGCAAG
    CGTATCCTGG TAGACTCGAA ATTCCTGGTA GGCCTGGGTG GGATTCTGGT AGTCGGCTGC
    TCTGTCATAG CCTCAATGGG TTTCTATGCT TGGATTGGTG TGGCGTCCTC GCTGGTCATT
    CTGCAGGTGG TGCCCTTCCT TGTACTGGCC GTGGGAGCTG ACAACATCTT CATTTTTGTC
    CTGGAGTATC AGAGAGACCT GCGCAGACCA GGAGAGCAGC GGGAGGAACA GATCGGCAGA
    GTTCTCGGAA ACGTGGCTCC CAGCATGCTG CTCTGCAGTT TGTCTGAATC TGTTTGTTTC
    TTCCTGGGAG CATTGAGCAC CATGCCAGCA GTGAAGTCTT TCGCTCTGTA CGCTGCATTG
    GCTGTGCTGA TGGACTTTGT TCTCCAGATG ACTGCCTTTG TGGCTCTGCT TTCTCTGGAT
    GCTCGCCGTC AGGACTCAAA CCGCTGTGAG ATAGCCTGCT GCGTGACAGT GGACACTCCA
    CGACCGCAAA AACCCAACGA GGGCATCCTG CTGCCGCTCA TGAGGAAATA CTACGCTCCG
    TTCCTACTCA ACTCTTTCAG CAGAGTAGTA GTGATGGTGT TGTTCCTGGT GATGTTCTTT
    AGCTCTGTGT TCCTCATGTT TCATGTCAAA GTGGGCTTGA ATCAGGAACT GGCCATGCCA
    ACTGACTCCT ACATGCTGGA CTACTTTGCA TACCTGTTTA AGTATTTTGA GGTTGGTGTG
    CCCACGTATT TCATCACGAC CAAAGGGTTC AACTTCAACA GCGAGGAGGG CATTAATGCA
    GTGTGTTCCA GTGTGGGATG TGACCAGTTC TCTATGACCC AGAAGATCCG CTACGCTACT
    GAATATCCTG AGCGATCTTA TCTGGCTATC CCAGCATCCT CTTGGGTGGA TGACTATATT
    GACTGGCTGA ACCCTGGATC CAGATGCTGT CGCCTTTACA CATTTGGTCC AAACAAAGAT
    GAATTTTGTC CTGCCAATGA GACCGGTGTC TGTCTCAAGA AGTGTATGGG CAAACCTGAA
    AATGGTGTAC TGCGGCCTGA TGTTTCAGAA TTCAACAGAT TTCTGCCAGA TTTTCTGAGC
    AACAGACCTG ACCTGCAGTG TCCAAAAGGT GGTTTGGGAG CCTATGATAA AGCTGTGATT
    AGAGACAATG AAACTGGGGA AATAATAGGT AAGATACATG CATTTTGTTT GTTTAATGGT
    CTTCTGACTG AGATTGGCTC TCTGGAGTGG GTGACTCACA CGTGTCAGAA GATCCCGGAG
    TCTCAGAGAA ATCACACATG TCTCTCTCAG GTGTTTGCTG GTGTTGCGAT GACCAATCTG
    CCCGGAATTG TTGTTTTGGC GTTTGCTAAA GCTCAGCTCA TCCAGATCTT CTTCTTCAGA
    CTCAATCTGG TCATAACTCT TCTCGGCATG GCCCATGGAC TCATATTTCT GCCTGTGCTG
    CTCAGTTACT TTGGTCCTGG AGTAAACAAC GCACTTCTGC TGCAGATTCA AGAAGAACAA
    GAAAAAGAGA TGGAGCTCAA TAGTAAAGCT ACATATGACA ACGTTTGCTT TGAAAACCAT
    GAAAAATCTA ACGGCGAAAT CCACGTAGAG CCTGCAAGTT TGCCTGAAAA CCATGAAAAG
    TCCAACGGCG TAATCCCTGC AGAGCCTTCA AGTTTCCCTG TAGAGCCTTC GAGCCCTCCA
    GAAGAGCCGT CAGTCCTAAT AATGGCAGTG ATTATGCCTG AAACCACCCA AATGCCCACC
    GAATGGATAT CTCCAAGTTG TAGCCCTAAA ACTTTCTTTG AGTTTATTTA CCATATCATT
    ATACTGAACT GGAAGTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302449.2
    CDS110..3970
    Translation

    Target ORF information:

    RefSeq Version XM_009304174.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NPC1-like 1 (npc1l1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCATTTCC AAAGGAAGAA CTCTGCGTGG TCCATTACTT TGATTTCACT TGCTGCTTGT 
    TTGGTTCTCA CAGGTGCTCA GCATGAACCA GGCTTCTGCG CAATGTATGA GGACTGTGGG
    TTAAATCCCG CAGTGGAAGG GGCTCTTATC CCCCCAAGAG TTCCCTGTAA GGACTACAGA
    AGAGCAGTGA ATGTGACAGG AGACCACTAT GAGCTTCTGA AATCTGTATG TCCAATGCTG
    GCCAATGGCG ATGGCCAAAC ATTGGCCTGC TGCTCCATCA GGCAACTCAC AGCTCTGCAA
    AACAGCCTGT CGCTCTCCAA AGCCGTTCTG ATCCGCTGCC CGTCCTGTGC CGAGAACTTT
    GCGTATCTCC ATTGCGCCAC GACCTGCAGT CCTGACCAAA GTCAGATATT GAAGATCACC
    AAAACGGCCA ACATCACTCA ACCCACAGGC ATTGTAAAAG AAGCCGTGGT GGGTTACGCA
    GCGTATATAT CCACTAACTT CTCTGATGCC TCGTTTGACT CCTGCAAAAA TGTACGAATT
    CCATCCACCG GAGGGTTTGC CATTGCCACA ATGTGCGGCC GTTATGGTGC AGCACTCTGT
    ACGCCACAGC GCTGGTATGA TTTTCAGGGC GACTCCAGCA ATGGATTGGC TCCGCTGGAT
    ATTGACTTCA AGCTTTTACA AGACAGCGAG ACCTCTGAAG TCCCAGCAGG AGCATTTGCG
    TTTGCAGGAC AAGCGTTAAA ATGCAATGAA GTCACACCTT CCGGTGGCGA GGCCTGTTCC
    TGTCAGGACT GCCTACAATC TTGTCCGGTG GTGCCAGAAC CACCGCCTCT GCCGAAACCC
    TTCATGATTG GTAACCTTAA TGGGGTGCTT GTCATCTGCC TCACAGTCTT CTCTTGCTTC
    CTTTTTCTTT TCCTGTGTTA CGTGATTACC GAGTGCACGA CTTACTACAT GAAGTCGAGG
    AAGGGTCGGA AGGGTAAGAG CACCAAGGAT CAAAACGCAA ATGAGATCAA ATTTAAAATC
    AGCCCTAAAG ATGTTTCGTG CTCTGACAAA GCCAGTCTTG CCACTCAGGA ATTCCTAGGG
    TCTCTTTTCC AGTCCTGGGG GACCTTAATG GCACGGTATC CCTACATAGT GCTTCCGGTC
    TGCCTTGTGA TTGTGCTGGT GTTTGCGGTG GGAATAAAGG ACATAGAACT GACCACAGAT
    CCCGTCCAGC TCTGGTCAGC ACCTGAAAGT CGCGCCATGC GGGAGAAGGC CTTCCACGAT
    GCTCATTTCG ATCCGTTTTA CCGCACCAAT CAGCTGATCT TGACGGCTCC AGGCCGGCCC
    AGCCACTTCT ATGACTCCTT ACTCTTTGGC AAGCAGAACT TCAGTGGCAT CATCTCCAAA
    GATTTGATCA TAGAGCTGTT AAAGCTTCAG CAGAGCATCC AAGCAATTGA GTTCTGGTCA
    GATGAACTAA ACAGTACCGC CAGCCTTAAG GATGTTTGCT ATGCGCCACT CAACCCAGGC
    AACCCCTCGT TAACCGACTG CGCTGTCAAC AGTCTGCCGC AGTACTTCCA AAATAGCATC
    GACAACCTGA ATGCCAAAGT GAACATGACA GAGCTTGGTG TGACTAAGGA AGTGGACTGG
    CGGGATCATT TCATCTACTG TGTTAACTCT CCACTATCAT TTAAAGACAT CACAGCTCTC
    GGCATGAGCT GTATGTCTGA CTATGGAGGC CCTGTGTTCC CCTTCCTGGC TGTCGGAGGA
    TATGATAATG AACAATACAC CACTGCTGAG GCCCTGATCC TCACTTTTTC CCTCAACAAT
    TATGCAAGAA CAGACGTTAA ATTTAAGGTG GTTGAAGAGT GGGAAAACCG GTTTCTTAAA
    ATCGTCCAGG AGTATCAGAA AAACTCTACA ACAAACTTCA CATTTGCTTA CATGGCTGAG
    AGGTCTTTGG AAGATGAGAT TAACAGAACG ACAGCGGAGG ATATCCCCAT ATTCATGATA
    AGCTATGCTG TCATCTTCCT CTACATCGCA GTGGCTCTTG GGGAATATTC ATCCTGCAAG
    CGTATCCTGG TAGACTCGAA ATTCCTGGTA GGCCTGGGTG GGATTCTGGT AGTCGGCTGC
    TCTGTCATAG CCTCAATGGG TTTCTATGCT TGGATTGGTG TGGCGTCCTC GCTGGTCATT
    CTGCAGGTGG TGCCCTTCCT TGTACTGGCC GTGGGAGCTG ACAACATCTT CATTTTTGTC
    CTGGAGTATC AGAGAGACCT GCGCAGACCA GGAGAGCAGC GGGAGGAACA GATCGGCAGA
    GTTCTCGGAA ACGTGGCTCC CAGCATGCTG CTCTGCAGTT TGTCTGAATC TGTTTGTTTC
    TTCCTGGGAG CATTGAGCAC CATGCCAGCA GTGAAGTCTT TCGCTCTGTA CGCTGCATTG
    GCTGTGCTGA TGGACTTTGT TCTCCAGATG ACTGCCTTTG TGGCTCTGCT TTCTCTGGAT
    GCTCGCCGTC AGGACTCAAA CCGCTGTGAG ATAGCCTGCT GCGTGACAGT GGACACTCCA
    CGACCGCAAA AACCCAACGA GGGCATCCTG CTGCCGCTCA TGAGGAAATA CTACGCTCCG
    TTCCTACTCA ACTCTTTCAG CAGAGTAGTA GTGATGGTGT TGTTCCTGGT GATGTTCTTT
    AGCTCTGTGT TCCTCATGTT TCATGTCAAA GTGGGCTTGA ATCAGGAACT GGCCATGCCA
    ACTGACTCCT ACATGCTGGA CTACTTTGCA TACCTGTTTA AGTATTTTGA GGTTGGTGTG
    CCCACGTATT TCATCACGAC CAAAGGGTTC AACTTCAACA GCGAGGAGGG CATTAATGCA
    GTGTGTTCCA GTGTGGGATG TGACCAGTTC TCTATGACCC AGAAGATCCG CTACGCTACT
    GAATATCCTG AGCGATCTTA TCTGGCTATC CCAGCATCCT CTTGGGTGGA TGACTATATT
    GACTGGCTGA ACCCTGGATC CAGATGCTGT CGCCTTTACA CATTTGGTCC AAACAAAGAT
    GAATTTTGTC CTGCCAATGA GACCGGTGTC TGTCTCAAGA AGTGTATGGG CAAACCTGAA
    AATGGTGTAC TGCGGCCTGA TGTTTCAGAA TTCAACAGAT TTCTGCCAGA TTTTCTGAGC
    AACAGACCTG ACCTGCAGTG TCCAAAAGGT GGTTTGGGAG CCTATGATAA AGCTGTGATT
    AGAGACAATG AAACTGGGGA AATAATAGGT AAGATACATG CATTTTGTTT GTTTAATGGT
    CTTCTGACTG AGATTGGCTC TCTGGAGTGG GTGACTCACA CGTGTCAGAA GATCCCGGAG
    TCTCAGAGAA ATCACACATG TCTCTCTCAG GTGTTTGCTG GTGTTGCGAT GACCAATCTG
    CCCGGAATTG TTGTTTTGGC GTTTGCTAAA GCTCAGCTCA TCCAGATCTT CTTCTTCAGA
    CTCAATCTGG TCATAACTCT TCTCGGCATG GCCCATGGAC TCATATTTCT GCCTGTGCTG
    CTCAGTTACT TTGGTCCTGG AGTAAACAAC GCACTTCTGC TGCAGATTCA AGAAGAACAA
    GAAAAAGAGA TGGAGCTCAA TAGTAAAGCT ACATATGACA ACGTTTGCTT TGAAAACCAT
    GAAAAATCTA ACGGCGAAAT CCACGTAGAG CCTGCAAGTT TGCCTGAAAA CCATGAAAAG
    TCCAACGGCG TAATCCCTGC AGAGCCTTCA AGTTTCCCTG TAGAGCCTTC GAGCCCTCCA
    GAAGAGCCGT CAGTCCTAAT AATGGCAGTG ATTATGCCTG AAACCACCCA AATGCCCACC
    GAATGGATAT CTCCAAGTTG TAGCCCTAAA ACTTTCTTTG AGTTTATTTA CCATATCATT
    ATACTGAACT GGAAGTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.