TGS1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TGS1
Entrez Gene ID	100537552
Full Name	trimethylguanosine synthase 1
Synonyms	si:ch73-38p6.4
General protein information	Preferred Names trimethylguanosine synthase 1 Names trimethylguanosine synthase
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163413.4	XP_005163470.1	trimethylguanosine synthase
XM_003197865.5	XP_003197913.2	trimethylguanosine synthase
XM_003197865.4	XP_003197913.2	trimethylguanosine synthase
XM_005163413.3	XP_005163470.1	trimethylguanosine synthase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TGS1	NP_001192627.1
Rattus norvegicus (Norway rat)	Tgs1	NP_001101374.1
	TGS1	XP_001084320.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010425	XP_001689406.1
Homo sapiens (human)	TGS1	NP_079107.6
Pan troglodytes (chimpanzee)	TGS1	XP_001153796.1
Gallus gallus (chicken)	TGS1	NP_001006389.1
Danio rerio (zebrafish)	LOC100537552	XP_005163470.1
Canis lupus familiaris (dog)	TGS1	XP_535077.2
Xenopus tropicalis (tropical clawed frog)	tgs1	XP_004915251.1
Mus musculus (house mouse)	Tgs1	NP_473430.3

Gene Ontology

The following TGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa07061	XM_005163413.4 Latest version!	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07061	XM_003197865.5 Latest version!	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa07061	XM_003197865.4	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07061	XM_005163413.3	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGS1 ( XM_005163413.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163470.1 Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07061
Clone ID Related Accession (Same CDS sequence)	XM_005163413.4 , XM_003197865.5 , XM_003197865.4 , XM_005163413.3
Accession Version	XM_005163413.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	trimethylguanosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163413.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163470.1
CDS	101..2503
Translation	MMIDYGERNVVPLADIIFYQGEETEMTIHCLCSRAYVNDRELYRSDFKAISSQADSQVQGDVETNDGEQLLEDLNCSVDEDEEEEEEEEDAAVDEETLLMASLGLPVQFASSSKQRRAGQVLARPKNKRTVKCLETSSDYTDANENSEDIAEPPCEQICPTSDEAWLRYWQQHGEGLLWQSWLEKHPENSSSTVQDFSHSQTDWDDHFQQIYKYYWEQFQYWAAQGWTLDESYSTTAASEENQNCQGETEALESETKDRSKADASSVTHLISSLSLLTEEVDMDDGRQPLVCSCADEPCDGGNRKRPSSRAACTDASDGNMKQTSGSAPMNNGSGRPTKSRSNDEDQSDDDDDDEPPGNRRAKVKRAHELDAEEIAEVPVEEAWDALGLRRGSQPKFESVVKLKAGQGSYTGRRREDRKPPEQRRKAACKINKHIFFTDDGPQTTKPKISKALQKVQSFLQQVQTDADSEDVATTSMPEALIGPFDKAELASLPTRKEDEEEASNDTDEEDCETTPQPSRICEEQQHIIPDLEEIHEDEADPRGELQPLDVPDFLLPDAHEDTKKESVQAFKKNKRKKRKRRKDVEMPPEIAAEPELAKYWAQRYRLFSRFDEGIKLDHEGWFSVTPEKIAEHIALRVQDCFNTELIIDAFCGVGGNAIQFALTGKRVIGIDIDPVRLALAQHNAAVYGVEQRIDFLQGDFLQLAPHLRGDMVFLSPPWGGPEYLTADVFNIQTMMTPDGFEIFRLSKMISDNIVYFLPRNADMEQIASLAGPGGKVEVEQNFLNNKLKTITAYFGNLIK

Target ORF information:

RefSeq Version	XM_005163413.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163413.4

ORF Insert Sequence:

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGS1 ( XM_003197865.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003197913.2 Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07061
Clone ID Related Accession (Same CDS sequence)	XM_005163413.4 , XM_003197865.5 , XM_003197865.4 , XM_005163413.3
Accession Version	XM_003197865.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	trimethylguanosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197865.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197913.2
CDS	275..2677
Translation	MMIDYGERNVVPLADIIFYQGEETEMTIHCLCSRAYVNDRELYRSDFKAISSQADSQVQGDVETNDGEQLLEDLNCSVDEDEEEEEEEEDAAVDEETLLMASLGLPVQFASSSKQRRAGQVLARPKNKRTVKCLETSSDYTDANENSEDIAEPPCEQICPTSDEAWLRYWQQHGEGLLWQSWLEKHPENSSSTVQDFSHSQTDWDDHFQQIYKYYWEQFQYWAAQGWTLDESYSTTAASEENQNCQGETEALESETKDRSKADASSVTHLISSLSLLTEEVDMDDGRQPLVCSCADEPCDGGNRKRPSSRAACTDASDGNMKQTSGSAPMNNGSGRPTKSRSNDEDQSDDDDDDEPPGNRRAKVKRAHELDAEEIAEVPVEEAWDALGLRRGSQPKFESVVKLKAGQGSYTGRRREDRKPPEQRRKAACKINKHIFFTDDGPQTTKPKISKALQKVQSFLQQVQTDADSEDVATTSMPEALIGPFDKAELASLPTRKEDEEEASNDTDEEDCETTPQPSRICEEQQHIIPDLEEIHEDEADPRGELQPLDVPDFLLPDAHEDTKKESVQAFKKNKRKKRKRRKDVEMPPEIAAEPELAKYWAQRYRLFSRFDEGIKLDHEGWFSVTPEKIAEHIALRVQDCFNTELIIDAFCGVGGNAIQFALTGKRVIGIDIDPVRLALAQHNAAVYGVEQRIDFLQGDFLQLAPHLRGDMVFLSPPWGGPEYLTADVFNIQTMMTPDGFEIFRLSKMISDNIVYFLPRNADMEQIASLAGPGGKVEVEQNFLNNKLKTITAYFGNLIK

Target ORF information:

RefSeq Version	XM_003197865.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197865.5

ORF Insert Sequence:

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGS1 ( XM_003197865.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003197913.2 Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07061
Clone ID Related Accession (Same CDS sequence)	XM_005163413.4 , XM_003197865.5 , XM_003197865.4 , XM_005163413.3
Accession Version	XM_003197865.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	trimethylguanosine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197913.2
CDS	280..2682
Translation	MMIDYGERNVVPLADIIFYQGEETEMTIHCLCSRAYVNDRELYRSDFKAISSQADSQVQGDVETNDGEQLLEDLNCSVDEDEEEEEEEEDAAVDEETLLMASLGLPVQFASSSKQRRAGQVLARPKNKRTVKCLETSSDYTDANENSEDIAEPPCEQICPTSDEAWLRYWQQHGEGLLWQSWLEKHPENSSSTVQDFSHSQTDWDDHFQQIYKYYWEQFQYWAAQGWTLDESYSTTAASEENQNCQGETEALESETKDRSKADASSVTHLISSLSLLTEEVDMDDGRQPLVCSCADEPCDGGNRKRPSSRAACTDASDGNMKQTSGSAPMNNGSGRPTKSRSNDEDQSDDDDDDEPPGNRRAKVKRAHELDAEEIAEVPVEEAWDALGLRRGSQPKFESVVKLKAGQGSYTGRRREDRKPPEQRRKAACKINKHIFFTDDGPQTTKPKISKALQKVQSFLQQVQTDADSEDVATTSMPEALIGPFDKAELASLPTRKEDEEEASNDTDEEDCETTPQPSRICEEQQHIIPDLEEIHEDEADPRGELQPLDVPDFLLPDAHEDTKKESVQAFKKNKRKKRKRRKDVEMPPEIAAEPELAKYWAQRYRLFSRFDEGIKLDHEGWFSVTPEKIAEHIALRVQDCFNTELIIDAFCGVGGNAIQFALTGKRVIGIDIDPVRLALAQHNAAVYGVEQRIDFLQGDFLQLAPHLRGDMVFLSPPWGGPEYLTADVFNIQTMMTPDGFEIFRLSKMISDNIVYFLPRNADMEQIASLAGPGGKVEVEQNFLNNKLKTITAYFGNLIK

Target ORF information:

RefSeq Version	XM_003197865.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197865.4

ORF Insert Sequence:

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGS1 ( XM_005163413.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163470.1 Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07061
Clone ID Related Accession (Same CDS sequence)	XM_005163413.4 , XM_003197865.5 , XM_003197865.4 , XM_005163413.3
Accession Version	XM_005163413.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	trimethylguanosine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163413.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163470.1
CDS	94..2496
Translation	MMIDYGERNVVPLADIIFYQGEETEMTIHCLCSRAYVNDRELYRSDFKAISSQADSQVQGDVETNDGEQLLEDLNCSVDEDEEEEEEEEDAAVDEETLLMASLGLPVQFASSSKQRRAGQVLARPKNKRTVKCLETSSDYTDANENSEDIAEPPCEQICPTSDEAWLRYWQQHGEGLLWQSWLEKHPENSSSTVQDFSHSQTDWDDHFQQIYKYYWEQFQYWAAQGWTLDESYSTTAASEENQNCQGETEALESETKDRSKADASSVTHLISSLSLLTEEVDMDDGRQPLVCSCADEPCDGGNRKRPSSRAACTDASDGNMKQTSGSAPMNNGSGRPTKSRSNDEDQSDDDDDDEPPGNRRAKVKRAHELDAEEIAEVPVEEAWDALGLRRGSQPKFESVVKLKAGQGSYTGRRREDRKPPEQRRKAACKINKHIFFTDDGPQTTKPKISKALQKVQSFLQQVQTDADSEDVATTSMPEALIGPFDKAELASLPTRKEDEEEASNDTDEEDCETTPQPSRICEEQQHIIPDLEEIHEDEADPRGELQPLDVPDFLLPDAHEDTKKESVQAFKKNKRKKRKRRKDVEMPPEIAAEPELAKYWAQRYRLFSRFDEGIKLDHEGWFSVTPEKIAEHIALRVQDCFNTELIIDAFCGVGGNAIQFALTGKRVIGIDIDPVRLALAQHNAAVYGVEQRIDFLQGDFLQLAPHLRGDMVFLSPPWGGPEYLTADVFNIQTMMTPDGFEIFRLSKMISDNIVYFLPRNADMEQIASLAGPGGKVEVEQNFLNNKLKTITAYFGNLIK

Target ORF information:

RefSeq Version	XM_005163413.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio trimethylguanosine synthase 1 (tgs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163413.3

ORF Insert Sequence:

ATGATGATTG ATTACGGCGA GCGGAATGTT GTTCCTCTTG CTGATATCAT CTTCTATCAA 
GGCGAAGAGA CAGAGATGAC TATACACTGC TTGTGCTCCA GAGCCTACGT TAATGATCGA 
GAATTGTATC GGTCAGATTT TAAAGCCATA TCAAGTCAAG CTGACAGTCA AGTGCAAGGA 
GATGTTGAAA CTAATGATGG GGAACAGCTT TTAGAAGATC TTAACTGTTC TGTGGATGAA 
GATGAGGAAG AGGAGGAGGA AGAAGAAGAT GCTGCTGTTG ATGAAGAGAC CTTGCTCATG 
GCCAGTTTGG GGCTTCCTGT GCAGTTTGCC AGCTCATCAA AACAGAGGAG AGCTGGCCAA 
GTTTTAGCAA GACCGAAGAA CAAAAGAACC GTCAAGTGTT TGGAAACCTC TTCTGATTAT 
ACAGACGCAA ATGAAAACTC TGAGGACATC GCAGAGCCTC CCTGTGAACA GATTTGTCCC 
ACATCAGACG AGGCCTGGTT GAGGTACTGG CAACAGCATG GAGAGGGTCT GCTGTGGCAA 
AGCTGGTTGG AGAAACATCC TGAAAACTCC AGCAGTACAG TACAAGACTT TTCCCACTCA 
CAGACAGACT GGGACGACCA CTTCCAACAG ATATACAAGT ACTACTGGGA ACAGTTTCAG 
TACTGGGCCG CACAGGGCTG GACATTAGAC GAGTCCTACA GCACAACTGC TGCTTCTGAG 
GAAAATCAAA ACTGCCAGGG AGAAACGGAG GCACTCGAGA GTGAGACAAA AGACAGATCG 
AAGGCGGATG CAAGCAGTGT TACACATCTC ATAAGCAGCT TGAGCTTATT GACCGAGGAA 
GTTGACATGG ATGATGGCCG GCAGCCTTTG GTCTGTAGCT GCGCCGATGA GCCCTGCGAT 
GGTGGAAACC GCAAAAGGCC CTCGTCACGT GCAGCCTGCA CTGATGCTTC TGATGGCAAC 
ATGAAACAGA CCTCTGGCTC TGCGCCGATG AATAATGGAA GTGGACGACC TACAAAGAGC 
AGATCTAATG ATGAGGATCA GAGCGATGAT GATGATGATG ATGAACCTCC AGGGAACCGA 
CGAGCTAAAG TGAAGAGAGC TCATGAGCTG GATGCCGAGG AGATTGCGGA GGTACCAGTC 
GAGGAGGCTT GGGATGCTCT TGGCCTAAGA CGGGGTTCAC AGCCAAAGTT TGAGAGTGTG 
GTGAAGCTGA AAGCAGGCCA GGGTTCATAC ACAGGCAGAC GGCGAGAGGA CAGAAAGCCC 
CCTGAGCAAA GAAGAAAAGC AGCCTGCAAG ATCAACAAGC ACATCTTCTT TACAGATGAT 
GGGCCGCAAA CCACAAAGCC AAAGATCAGC AAAGCTCTTC AAAAAGTCCA GAGTTTCCTC 
CAGCAGGTCC AGACTGATGC AGACTCTGAG GACGTGGCCA CAACATCCAT GCCTGAAGCT 
CTTATTGGGC CATTTGACAA AGCGGAATTG GCCAGTCTAC CTACACGAAA AGAAGATGAA 
GAAGAAGCCA GTAATGATAC AGATGAAGAA GACTGCGAAA CGACTCCACA ACCAAGTAGA 
ATTTGTGAGG AACAACAGCA TATCATACCA GATCTAGAAG AAATACATGA GGATGAAGCT 
GATCCCAGAG GAGAACTGCA GCCGCTGGAT GTCCCTGACT TCTTGCTCCC TGATGCTCAT 
GAAGACACTA AGAAAGAGAG TGTGCAGGCC TTTAAAAAGA ACAAGAGGAA GAAGAGGAAG 
AGGAGGAAGG ATGTGGAGAT GCCACCTGAG ATTGCAGCAG AACCTGAGCT GGCCAAATAC 
TGGGCTCAGC GATACCGTCT GTTTTCCAGG TTCGATGAAG GCATCAAACT TGACCACGAG 
GGGTGGTTTT CAGTGACTCC TGAGAAGATC GCGGAGCACA TCGCTCTGAG GGTGCAGGAC 
TGCTTCAACA CAGAGCTCAT TATCGATGCC TTCTGTGGTG TGGGTGGAAA TGCCATTCAG 
TTTGCCCTCA CTGGTAAAAG AGTGATCGGC ATTGATATTG ACCCGGTGCG TCTGGCTCTG 
GCGCAGCACA ATGCTGCAGT GTACGGCGTG GAGCAACGCA TAGACTTCTT GCAGGGAGAT 
TTCCTTCAGC TGGCTCCACA TCTGCGAGGA GACATGGTTT TCCTCAGTCC GCCGTGGGGA 
GGGCCAGAAT ATCTAACCGC TGATGTCTTC AACATCCAGA CCATGATGAC TCCTGATGGA 
TTTGAAATTT TCAGGTTGTC AAAAATGATA TCGGACAATA TTGTTTATTT TCTACCTCGA 
AACGCAGACA TGGAGCAGAT CGCGTCTCTC GCCGGACCAG GTGGAAAGGT AGAAGTCGAG 
CAGAATTTTC TGAACAACAA GCTGAAGACG ATCACGGCGT ACTTTGGGAA TTTAATCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TGS1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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