si:ch211-156l18.8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch211-156l18.8
Entrez Gene ID	100537469
Full Name	si:ch211-156l18.8
Synonyms	gpr179
General protein information	Preferred Names si:ch211-156l18.8 Names probable G-protein coupled receptor 158 probable G-protein coupled receptor 179 G protein-coupled receptor 179
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358296.1	XP_017213785.1	probable G-protein coupled receptor 179
XM_021472395.1	XP_021328070.1	probable G-protein coupled receptor 158 isoform X3
XM_017358296.2	XP_017213785.2	probable G-protein coupled receptor 158 isoform X1
XM_021479749.1	XP_021335424.1	probable G-protein coupled receptor 158 isoform X1
XM_021472410.1	XP_021328085.1	probable G-protein coupled receptor 179 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GPR179	NP_001178173.1
Rattus norvegicus (Norway rat)	Gpr179	XP_006247545.1
Pan troglodytes (chimpanzee)	GPR179	XP_511435.2
Canis lupus familiaris (dog)	GPR179	XP_548160.2
Xenopus tropicalis (tropical clawed frog)	gpr179	XP_002940200.2
	GPR179	XP_001081991.1
Mus musculus (house mouse)	Gpr179	NP_001074689.1
Danio rerio (zebrafish)	LOC100537469	XP_003199496.1
Homo sapiens (human)	GPR179	NP_001004334.2
Danio rerio (zebrafish)	LOC101884475	XP_005155818.1
Danio rerio (zebrafish)	LOC101884615	XP_005174463.1

Gene Ontology

The following si:ch211-156l18.8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-156l18.8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145223	XM_021472395.1 Latest version!	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa145237	XM_021472410.1 Latest version!	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa146632	XM_017358296.2 Latest version!	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa50523	XM_021479749.1 Latest version!	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa54322	XM_017358296.1 Latest version!	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch211-156l18.8 ( XM_021472395.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328070.1 Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa145223
Clone ID Related Accession (Same CDS sequence)	XM_021472395.1
Accession Version	XM_021472395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCCATTCC ACAACAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CGTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328070.1
CDS	1492..3357
Translation	MGPSAGVLPLLLLLPRLLCAQLSTVSELKDTSSLEFTPTPFLTDPNITAGKGLSPSPQATSLPPVTVEDRSAAESYLYNGDSSSLAASTCTRTFQLPVRRGPPPRDLLPLLHQAIASLTNAANFLNLIFQASELRETSVREDIEWYHALVRTMLEGDRPGLVRRAFLTFDADPTIQQPQLVLRASKGTTQDILLQDLTSAWTSFRPPAPDQSWFDIFKSSSPPLHALSKRVLLNDLSTLDTPKWARGDSYVINSSGVQWGEGPFLECVDGRFLPGWLLSLSMPFYGLKPDLSPEFRGVIRVDVNIQGFSVDQCATGDFWFANTHQCNRTSMECEPIPQQGFRMGQYCCRCKKGYYSPISDIQNKRNNRENNTQACYPEVPVCVPCWPGCAECEDGAPCWVQEDWLLRTAVLAVQGLFMILVLISMLVAYQCRKTKRIRSSGLLLLEMILCGSLLLYFPVFILYFKPSTFRCILLRWVRLLGFAIVYGTVALKMYRVLKVFLSRTAQRVPYITTTGVLRMLGVMVLTVSWFLCAWTVGVLQNRDRNVPLLIISTTSEGQGFNVCDLDRWDYMMAVAELLFLCWGSFLCSAVKTVPSAFHEPRYMSIAIHNELLLSSMFHLLR

Target ORF information:

RefSeq Version	XM_021472395.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472395.1

ORF Insert Sequence:

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCCATTCC ACAACAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CGTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-156l18.8 ( XM_021472410.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328085.1 Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145237
Clone ID Related Accession (Same CDS sequence)	XM_021472410.1
Accession Version	XM_021472410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 179 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394804.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGC TGTTCTTCAC TCACACGCAT GTCACTATTA CTGTTACACT CGGCCTGCTG 
TTTGTACCCA AGTTTCTCCA TATGTCACAA CCAGGCAGAG AAGAAATAGC GGCAGAGGTA 
TATGAGGATG AGGTGGACTT GCGACGTTCG TGTTCTTACC TCAACAGTAG CTTCACCTCG 
AACTGCTGGA GTGACCAAAG TTTGGACCCT GATGACATTC GGGATGAGCT GAAGAAACTG 
TATTCTCAGC TGGAAGTCCA CAAGACCAAG AGGATGGCAA ACAGCAATCC CCACCTTCCA 
AAGAAGCGTA GCTCTCGTCT CAGTATTGGA CGATCTATTA TAAAACGTAT CTCTGAGATC 
CCAGAAAGTT TGAGCAGACA ATGTAGCCGT GAAGACAAGG ATATCAGTGC TGCAATAGGA 
ACTATAACAC GATCGGGATC TTATTACAAA TGTCATCAAA CCACAAGTAT TAACACTATA 
AATGAAACTT TAATGCAGCC TTCTCCAAAG CTGCGGAAGT CCCATAGTGC CTATGATTGT 
ACCCCAGAAA AGGAAGCCTC TTTATTAAAC TCCTCCATGA AAAGATCAAC AGAAAAGAGG 
CACTCACAGC ATTCTGATGC AGGATCTATC AATACAACAC TGCTGATCTG CAAGTCTGCC 
AGTGCCCACA ATCTATCGCT AGATACTAAC CTTTTACTTC CCGAGCCCAC CAGGTTTCAA 
AAGTCACTTA GTGTAATAGA ACGCAATGAC CATCGCTCTG TGACACCGTG TAGAAGCATT 
GAAAATGTGT CAATTTCTAT CAAACCTACC CCAGCAGAAA GGCCACCCAA GCTGACTGTG 
GATGTTGACA TAAAGAGGCA GACTTCTAGT GCCTTGCTGT CAGAGTCCTT TGACAAGGCA 
GAGGTCTGCC CATGGGAGGT TCCAGAGGAG CTCCCTGCAA CCAAAAATCT AAAACATGTC 
ACATATTCCA TCTCAACACA GGAAGAACCA AAATCGCCTG GTGCTCTGTC ATCACCAACA 
TTGTTATACG TTTGTCCGTG GGAATACCTG CCACCTCCTT CTCCCCCTGC AATTGAAACT 
GGGAATGGTA TTCAGTCAGA AGTTGATTCC ACAAAACCCA AACCTCCAAT ATCTTGTAGT 
GCTCCTGGGT CACCACATAC AGTTTCTGGC AAGCCAAAAG AATTTCGGGT TTTCTCTTTC 
CGCTCCACTA CACAAAATCT CCTTGCAGCA AAGGGTGTTG GGGAACTTGC AAGAAGCATG 
AGCAGAGAGA AAAGCCTACA AGAAAGTAAC GTTAGGGAGG GAACACTACA AAAATCTCAG 
TCTACATCCA TGAGATTATC TCCAGGCAGT GCAGCTTCAG ACAAACGTAC CCCACAGACA 
CAAAGACGGG CTATGACTAC TATAGGAACA AAACCTTCCC TTGTGAAACA AACAGCTGTA 
CGGCTGCCAT CAAATGATTC TCCCGAAAGG AGCCCCAAGC ATACTGCTCC AGTTCACGTC 
TGCCCATGGG AGTCAGAGGA GGTGATCTTG GAGGTCAAAA AGCAGAATGA TATAGCATTA 
CAAAGACAAC TGAGTGACAA GAAAGAATTG ACTGTGACAG CAAGTAACGA TGGTTTAAAG 
CCATCTGAGC TACAACTTAA GTCATTATTA GTTGTTCCTA ATACAGAAGT GTGTCCATGG 
GATGTCCCAA AACCTTTTCA TCAAAGTAGC ATGTCTGAAG TCTGTCCTTG GGAGGTTGTG 
GAAGTCGAGC AATTGCAAAC CAAAAACATG CAAACTGATG TCTGTCCTTG GGAAACAGAC 
TCATCAAGGG TTACTGAAGA CACAATATGC AATACCACTG TTGAAGTACC CATGCCTGGA 
GACATCCAGA AGCAGGTAAA AGTGAAGCCT GAAACATCCC CTTCTGATGT ATCAGATACA 
AATATACCCA AGGTCAAACA AGGATCCATA AAAATGTCCA ATGAGACAAA GCCTGGTGAA 
AGGGTTATTT ATGTTAATAC CAGAGAGTGT CGGTTGGAAC AAGAATCGGC TCAGAAAAGA 
GGATTTATGG AAACAAGTAA AACACTGAAG CAAGAAGAAA GTGATTTTCC TGAACCACCC 
CCACCTGTAA CTGATGTATG TCCTTGGGAT TTAGAGCCAA ATACAAAAGA CAATTCAAAA 
CCCTCAATGA CTAAAAAAGC AACTGTTTGT CCGTGGGAGG CAGGGGATTC TGAAAAGTCA 
GAAGAGAGGG AAAGTGCTAC AATCAAGGGG TCAGCAAAAG TACATCAAAA ACAAGAAAGT 
GTATATGCAA ATGTCTGTCC ATGGGAAACA AGCGAGTCTG TTAAGACCAT GCAGAAACAA 
GATAGTATCA GTAGAAATGT CTGTCCATGG GAGACACAAG AGTCAGTGAA GACTTTGCAG 
AAACAAGATA GTGTCCGTAC AAATGTTTGT CCATGGGAGA CAGAAGACGG GGTAAAAACC 
TTACAGAAAC AAGATAGTGT CCGTACAGAT GTTTGTCCAT GGGAAACAGA AGAATCTGTC 
AAGACCTTGC AGAAACAAGA TAGTGTCCGT ACAAATGTTT GTCCATGGGA AACAGAAGAA 
TCTGTCAAGA CCTTGCCGAA ACAAGATAGT GTCCGTACAA ATGTTTGTCC ATGGGAGACA 
GAAGACCAGG TAAAAACCTT ACAGAAACAA GATAGTGTCC GTACAGATGT TTGTCCATGG 
GAGACAGAAG AGCCAGTCAA GACCTTGCAG AAACAGGATA GTGTCCGTAC AAATGTTTGT 
CCATGGGAGA CAGAAGAGAC GGTAAAGTCC TTGCAGAAGC AAGACAGTGT CTGTACAAAT 
GTTTGTCCTT GGGAGACAAG CAAGTCTGTT AAGACCATAC AGAAACAAGA CAGTGTCCGT 
ACAAATGTAT GTCCATGGGA GACAGAAGAG CCAGTCAAGA CCTTGCAGAA ACAAGAAAGT 
GTCCATGCAG AAGTTTGTCC ATGGGAGACA GAAGAGCCTG AAAAGGCCTT GCAGAAACAA 
GACAGTGTTC TTTCAGATGT TTGTCCATGG GAGAAAAATG AGCCAGTGAA GACCTTACAG 
AAGCAAGATC GTGTCTGTTC AGATGTCTGT CCATGGGAAT CAAACACATC AGCGATTCAA 
TCAGTGCCAC AAGACTCCCC TCGTACGGTC CCAACAAAAA GTCAAGAAAG TGCTCAAGAA 
TCTGTCTGTC CCTGGGAAAC TAACATGGGA ACAACAGCAA CAACAGAAGC CAGCACAAGT 
CAAACTGTCC TAGACACAAT CAGAACACAC CCTTCAGACA CACAGACTGC AGAAGAGTTA 
CGGGTTAACC CTCCTCTAGC TAGACGTGAT GCATTATGTC CCTGGGAAAT GGAAGGGGGG 
CGACAAGAAT CTATAACCAT GCATGATAAT TTAGATGTCT TCACTTGGGA GGAGACAATC 
CCAGAGGAAG ATGATGGTGA TGCAGAAACT GCTGCAGAGG CCTTCATCTT CCCTCCAGAC 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328085.1
CDS	97..3582
Translation	MLLLFFTHTHVTITVTLGLLFVPKFLHMSQPGREEIAAEVYEDEVDLRRSCSYLNSSFTSNCWSDQSLDPDDIRDELKKLYSQLEVHKTKRMANSNPHLPKKRSSRLSIGRSIIKRISEIPESLSRQCSREDKDISAAIGTITRSGSYYKCHQTTSINTINETLMQPSPKLRKSHSAYDCTPEKEASLLNSSMKRSTEKRHSQHSDAGSINTTLLICKSASAHNLSLDTNLLLPEPTRFQKSLSVIERNDHRSVTPCRSIENVSISIKPTPAERPPKLTVDVDIKRQTSSALLSESFDKAEVCPWEVPEELPATKNLKHVTYSISTQEEPKSPGALSSPTLLYVCPWEYLPPPSPPAIETGNGIQSEVDSTKPKPPISCSAPGSPHTVSGKPKEFRVFSFRSTTQNLLAAKGVGELARSMSREKSLQESNVREGTLQKSQSTSMRLSPGSAASDKRTPQTQRRAMTTIGTKPSLVKQTAVRLPSNDSPERSPKHTAPVHVCPWESEEVILEVKKQNDIALQRQLSDKKELTVTASNDGLKPSELQLKSLLVVPNTEVCPWDVPKPFHQSSMSEVCPWEVVEVEQLQTKNMQTDVCPWETDSSRVTEDTICNTTVEVPMPGDIQKQVKVKPETSPSDVSDTNIPKVKQGSIKMSNETKPGERVIYVNTRECRLEQESAQKRGFMETSKTLKQEESDFPEPPPPVTDVCPWDLEPNTKDNSKPSMTKKATVCPWEAGDSEKSEERESATIKGSAKVHQKQESVYANVCPWETSESVKTMQKQDSISRNVCPWETQESVKTLQKQDSVRTNVCPWETEDGVKTLQKQDSVRTDVCPWETEESVKTLQKQDSVRTNVCPWETEESVKTLPKQDSVRTNVCPWETEDQVKTLQKQDSVRTDVCPWETEEPVKTLQKQDSVRTNVCPWETEETVKSLQKQDSVCTNVCPWETSKSVKTIQKQDSVRTNVCPWETEEPVKTLQKQESVHAEVCPWETEEPEKALQKQDSVLSDVCPWEKNEPVKTLQKQDRVCSDVCPWESNTSAIQSVPQDSPRTVPTKSQESAQESVCPWETNMGTTATTEASTSQTVLDTIRTHPSDTQTAEELRVNPPLARRDALCPWEMEGGRQESITMHDNLDVFTWEETIPEEDDGDAETAAEAFIFPPDL

Target ORF information:

RefSeq Version	XM_021472410.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472410.1

ORF Insert Sequence:

ATGCTCTTGC TGTTCTTCAC TCACACGCAT GTCACTATTA CTGTTACACT CGGCCTGCTG 
TTTGTACCCA AGTTTCTCCA TATGTCACAA CCAGGCAGAG AAGAAATAGC GGCAGAGGTA 
TATGAGGATG AGGTGGACTT GCGACGTTCG TGTTCTTACC TCAACAGTAG CTTCACCTCG 
AACTGCTGGA GTGACCAAAG TTTGGACCCT GATGACATTC GGGATGAGCT GAAGAAACTG 
TATTCTCAGC TGGAAGTCCA CAAGACCAAG AGGATGGCAA ACAGCAATCC CCACCTTCCA 
AAGAAGCGTA GCTCTCGTCT CAGTATTGGA CGATCTATTA TAAAACGTAT CTCTGAGATC 
CCAGAAAGTT TGAGCAGACA ATGTAGCCGT GAAGACAAGG ATATCAGTGC TGCAATAGGA 
ACTATAACAC GATCGGGATC TTATTACAAA TGTCATCAAA CCACAAGTAT TAACACTATA 
AATGAAACTT TAATGCAGCC TTCTCCAAAG CTGCGGAAGT CCCATAGTGC CTATGATTGT 
ACCCCAGAAA AGGAAGCCTC TTTATTAAAC TCCTCCATGA AAAGATCAAC AGAAAAGAGG 
CACTCACAGC ATTCTGATGC AGGATCTATC AATACAACAC TGCTGATCTG CAAGTCTGCC 
AGTGCCCACA ATCTATCGCT AGATACTAAC CTTTTACTTC CCGAGCCCAC CAGGTTTCAA 
AAGTCACTTA GTGTAATAGA ACGCAATGAC CATCGCTCTG TGACACCGTG TAGAAGCATT 
GAAAATGTGT CAATTTCTAT CAAACCTACC CCAGCAGAAA GGCCACCCAA GCTGACTGTG 
GATGTTGACA TAAAGAGGCA GACTTCTAGT GCCTTGCTGT CAGAGTCCTT TGACAAGGCA 
GAGGTCTGCC CATGGGAGGT TCCAGAGGAG CTCCCTGCAA CCAAAAATCT AAAACATGTC 
ACATATTCCA TCTCAACACA GGAAGAACCA AAATCGCCTG GTGCTCTGTC ATCACCAACA 
TTGTTATACG TTTGTCCGTG GGAATACCTG CCACCTCCTT CTCCCCCTGC AATTGAAACT 
GGGAATGGTA TTCAGTCAGA AGTTGATTCC ACAAAACCCA AACCTCCAAT ATCTTGTAGT 
GCTCCTGGGT CACCACATAC AGTTTCTGGC AAGCCAAAAG AATTTCGGGT TTTCTCTTTC 
CGCTCCACTA CACAAAATCT CCTTGCAGCA AAGGGTGTTG GGGAACTTGC AAGAAGCATG 
AGCAGAGAGA AAAGCCTACA AGAAAGTAAC GTTAGGGAGG GAACACTACA AAAATCTCAG 
TCTACATCCA TGAGATTATC TCCAGGCAGT GCAGCTTCAG ACAAACGTAC CCCACAGACA 
CAAAGACGGG CTATGACTAC TATAGGAACA AAACCTTCCC TTGTGAAACA AACAGCTGTA 
CGGCTGCCAT CAAATGATTC TCCCGAAAGG AGCCCCAAGC ATACTGCTCC AGTTCACGTC 
TGCCCATGGG AGTCAGAGGA GGTGATCTTG GAGGTCAAAA AGCAGAATGA TATAGCATTA 
CAAAGACAAC TGAGTGACAA GAAAGAATTG ACTGTGACAG CAAGTAACGA TGGTTTAAAG 
CCATCTGAGC TACAACTTAA GTCATTATTA GTTGTTCCTA ATACAGAAGT GTGTCCATGG 
GATGTCCCAA AACCTTTTCA TCAAAGTAGC ATGTCTGAAG TCTGTCCTTG GGAGGTTGTG 
GAAGTCGAGC AATTGCAAAC CAAAAACATG CAAACTGATG TCTGTCCTTG GGAAACAGAC 
TCATCAAGGG TTACTGAAGA CACAATATGC AATACCACTG TTGAAGTACC CATGCCTGGA 
GACATCCAGA AGCAGGTAAA AGTGAAGCCT GAAACATCCC CTTCTGATGT ATCAGATACA 
AATATACCCA AGGTCAAACA AGGATCCATA AAAATGTCCA ATGAGACAAA GCCTGGTGAA 
AGGGTTATTT ATGTTAATAC CAGAGAGTGT CGGTTGGAAC AAGAATCGGC TCAGAAAAGA 
GGATTTATGG AAACAAGTAA AACACTGAAG CAAGAAGAAA GTGATTTTCC TGAACCACCC 
CCACCTGTAA CTGATGTATG TCCTTGGGAT TTAGAGCCAA ATACAAAAGA CAATTCAAAA 
CCCTCAATGA CTAAAAAAGC AACTGTTTGT CCGTGGGAGG CAGGGGATTC TGAAAAGTCA 
GAAGAGAGGG AAAGTGCTAC AATCAAGGGG TCAGCAAAAG TACATCAAAA ACAAGAAAGT 
GTATATGCAA ATGTCTGTCC ATGGGAAACA AGCGAGTCTG TTAAGACCAT GCAGAAACAA 
GATAGTATCA GTAGAAATGT CTGTCCATGG GAGACACAAG AGTCAGTGAA GACTTTGCAG 
AAACAAGATA GTGTCCGTAC AAATGTTTGT CCATGGGAGA CAGAAGACGG GGTAAAAACC 
TTACAGAAAC AAGATAGTGT CCGTACAGAT GTTTGTCCAT GGGAAACAGA AGAATCTGTC 
AAGACCTTGC AGAAACAAGA TAGTGTCCGT ACAAATGTTT GTCCATGGGA AACAGAAGAA 
TCTGTCAAGA CCTTGCCGAA ACAAGATAGT GTCCGTACAA ATGTTTGTCC ATGGGAGACA 
GAAGACCAGG TAAAAACCTT ACAGAAACAA GATAGTGTCC GTACAGATGT TTGTCCATGG 
GAGACAGAAG AGCCAGTCAA GACCTTGCAG AAACAGGATA GTGTCCGTAC AAATGTTTGT 
CCATGGGAGA CAGAAGAGAC GGTAAAGTCC TTGCAGAAGC AAGACAGTGT CTGTACAAAT 
GTTTGTCCTT GGGAGACAAG CAAGTCTGTT AAGACCATAC AGAAACAAGA CAGTGTCCGT 
ACAAATGTAT GTCCATGGGA GACAGAAGAG CCAGTCAAGA CCTTGCAGAA ACAAGAAAGT 
GTCCATGCAG AAGTTTGTCC ATGGGAGACA GAAGAGCCTG AAAAGGCCTT GCAGAAACAA 
GACAGTGTTC TTTCAGATGT TTGTCCATGG GAGAAAAATG AGCCAGTGAA GACCTTACAG 
AAGCAAGATC GTGTCTGTTC AGATGTCTGT CCATGGGAAT CAAACACATC AGCGATTCAA 
TCAGTGCCAC AAGACTCCCC TCGTACGGTC CCAACAAAAA GTCAAGAAAG TGCTCAAGAA 
TCTGTCTGTC CCTGGGAAAC TAACATGGGA ACAACAGCAA CAACAGAAGC CAGCACAAGT 
CAAACTGTCC TAGACACAAT CAGAACACAC CCTTCAGACA CACAGACTGC AGAAGAGTTA 
CGGGTTAACC CTCCTCTAGC TAGACGTGAT GCATTATGTC CCTGGGAAAT GGAAGGGGGG 
CGACAAGAAT CTATAACCAT GCATGATAAT TTAGATGTCT TCACTTGGGA GGAGACAATC 
CCAGAGGAAG ATGATGGTGA TGCAGAAACT GCTGCAGAGG CCTTCATCTT CCCTCCAGAC 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-156l18.8 ( XM_017358296.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213785.2 Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.

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CloneID	ODa146632
Clone ID Related Accession (Same CDS sequence)	XM_017358296.2
Accession Version	XM_017358296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395295.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCTATTCC ACAAAAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGATTTGCCA GACCGTCAAT TCATCCTGAC TGGATGCTCT TGCTGTTCTT CACTCACACG 
CATGTCACTA TTACTGTTAC ACTCGGCCTG CTGTTTGTAC CCAAGTTTCT CCATATGTCA 
CGACCAGGCA GAGAAGAAAT AGCGGCAGAA GTATATGAGG ATGAGGTGGA CTTGCGACGT 
TCGTGTTCTT ACCTCAACAG TAGCTTCACC TCGAACTGCT GGAGTGACCA AAGTTTGGAC 
CCTGATGACA TTCGGGATGA GCTGAAGAAA CTGTATTCTC AGCTGGAAGT CCACAAGACC 
AAGAGGATGG CAAACAGCAA TCCCCACCTT CCAAAGAAGC GTAGCTCTCG TCTCAGTATT 
GGACGATCTA TTATAAAACG TATCTCTGAG ATCCCAGAAA GTTTGAGCAG ACAATGTAGC 
CGTGAAGACA AGGATATCAG TGCTGCAATA GGAACTATAA CACGATCGGG ATCTTATTAC 
AAATGTCATC AAACCACAAG TATTAACACT ATAAATGAAA CTTTAATGCA GCCTTCTCCA 
AAGCTGCGGA AGTCCCATAG TGCCTATGAT TGTACCCCAG AAAAGGAAGC CTCTCTATTA 
AACTCCTCCA TGAAGAGATC AACAGAAAAG AGGCACTCAC AGCATTCTGA TGCAGGATCT 
ATCAATACAA CACTGCTGAT CTGCAAGTCT GCCAGTGCCC ACAATCTATC GCTAGATACT 
AACCTTTTAC TTCCCGAGCC CACCAGGTTT CAAAAGTCAC TTAGTGTAAT AGAACGCAAT 
GACCATCGCT CTGTGACACC GTGTAGAAGC ATTGAAAATG TGTCAATTTC TATCAAACCT 
ACCCCAGCAG AAAGGCCACC CAAGCTGACT GTGGATGTTG ACATAAAAAG GCAGACTTCT 
AGTGCCTTGC TGTCAGAGTC CTTTGACAAG GCAGAGGTCT GCCCATGGGA GGTTCCAGAG 
GAGCTCCCTG CAAACAAAAA TCTAAAACAT GTCACATATT CAATCTCAAC ACAGGAAGAA 
CCAAAATCGC CTGGTGCTCT GTCATCACCA ACATTGTTAT ACGTTTGTCC GTGGGAATAC 
CTGCCACCTC CTTCTCCCCC TGCAATTGAA ACTGGGAATG GTATTCAGTC AGAAGTTGAT 
TCCACAAAAC CCAAACCTCC AATATCTTGT AGTGCTCCTG GGTCACCACA TACAGTTTCT 
GGCAAGCCAA AAGAATTTCG GGTTTTCTCT TTCCGCTCCA CTACACAAAA TCTCCTTGCA 
GCAAAGGGTG TTGGGGAACT TGCAAGAAGC ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT 
AACGTTAGGG AGGGAACACT ACAAAAATCT CAGTCTACAT CCATGAGATT ATCTCCAGGC 
AGTGCAGCTT CAGACAAACG TACCCCACAG ACACAAAGAC GGGCTATGAC TACTATAGGA 
ACAAAACCTT CCCTTGTGAA ACAAACAGCT GTACGGCTGC CATCAAATGA TTCTCCCGAA 
AGGAGCCCCA AGCATACTGC TCCAGTTCAC GTCTGCCCAT GGGAGTCAGA GGAGGTGATC 
TTGGAGGTCA AAAAGCAGAA TGATACAGCA TTACAAAGAC AACTGAGTGA CAAGAAAGAA 
TTGACTGTGA CAGCAAGTAA CGATGGTTTA AAGCCATCTG AGCTACAACT TAAGTCATTA 
TTAGTTGTTC CTAATACAGA AGTGTGTCCA TGGGATGTCC CAAAACCTTT TCATCAAAGT 
AGCATGTCTG AAGTCTGTCC TTGGGAGGTT GTGGAAGTCG AGCAATTGCA AACCAAAAAC 
ATGCAAACTG ATGTCTGTCC TTGGGAAACA GACTCATCAA GGGTTACTGA AGACACAATA 
TGCAATACCA CTGTTGAAGT ACCCATGCCT GGAGACATCC AGAAGCAGGT AAAAGTGAAG 
CCTGAAACAT CCCCTTCTGA TGTATCAGAT ACAAATATAC CCAAGGTCAA ACAAGGATCC 
ATAAAAATGT CCAATGAGAC AAAGCCTGGT GAAAGGGTTA TTTATGTTAA TACCAGAGAG 
TGTCGGTTGG AACAAGAATC GGCTCAGAAA AGAGGATTTA TGGAAACAAG TAAAACACTG 
AAGCAAGAAG AAAGTGATTT TCCTGAACCA CCCCCACCTG TAACTGATGT ATGTCCTTGG 
GATTTAGAGC CAAATACAAA AGACAATTCA AAACCCTCAA TGACTAAAAA AGCAACTGTT 
TGTCCGTGGG AGGCAGGGGA TTCTGAGAAG TCAGAAGAGA GGGAAAGTGC TACAATCAAG 
GGGTCAGCAA AAGTAGATCA AAAACAAGAA AGTGTATATG CAAATGTCTG TCCATGGGAA 
ACAAGCGAGT CTGTTAAGAC CATGCAGAAA CAAGATAGTA TCAGTAGAAA TGTCTGTCCA 
TGGGAGACAC AAGAGTCAGT GAAGACTTTG CAGAAACAAG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA CGGGGTAAAA ACCTTACAGA AACAAGATAG TGTCCGTACA 
GATGTTTGTC CATGGGAAAT AGAAGAATCT GTCAAGACCT TGCAGAAACA AGATAGTGTC 
CGTACAAATG TTTGTCCATG GGAAACAGAA GAATCTGTCA AGACCTTGCC GAAACAAGAT 
AGTGTCCGTA CAAATGTTTG TCCATGGGAG ACAGAAGACC AGGTAAAAAC CTTACAGAAA 
CAAGATAGTG TCCGTACAGA TGTTTGTCCA TGGGAGACAG AAGAGCCAGT CAAGACCTTG 
CAGAAACAGG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA GACGGTAAAG 
TCCTTGCAGA AGCAAGACAG TGTCTGTACA AATGTTTGTC CTTGGGAGAC AAGCAAGTCT 
GTTAAGACCA TACAGAAACA AGACAGTGTC CGTACAAATG TATGTCCATG GGAGACAGAA 
GAGCCAGTCA AGACCTTGCA GAAACAAGAA AGTGTCCATG CAGAAGTTTG TCCATGGGAG 
ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA CAAGACAGTG TTCTTTCAGA TGTTTGTCCA 
TGGGAGAAAA ACGAGCCAGT GAAGACCTTA CAGAAGCAAG ATCGTGTCTG TTCAGATGTC 
TGTCCATGGG AATCAAACAC ATCAGCGATT CAATCAGTGC CACAAGACTC CCCTCGTACG 
GTCCCAACAA AAAGTCAAGA AAGTGCTCAA GAATCTGTCT GTCCCTGGGA AACTAACATG 
GGAACAACAG CAACAACAGA AGCCAGCACA AGTCAAACTG TCCCAGACAC AATCAGAACA 
CACCCTTCAG ACACACAGAC TGCAGAAGAG TTACGGGTTA ACCCTCCTCT AGCTAGACGT 
GATGCATTAT GTCCCTGGGA AATGGAAGGG GGGCGACAAG AATCTATAAC CATGCATGAT 
AATTTAGATG TCTTCACTTG GGAGGAGACA ATCCCAGAGG AAGATGATGG TGATGCAGAA 
ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213785.2
CDS	1553..6931
Translation	MGPSAGVLPLLLLLPRLLCAQLSTVSELKDTSSLEFTPTPFLTDPNITAGKGLSPSPQATSLPPVTVEDRSAAESYLYNGDSSSLAASTCTRTFQLPVRRGPPPRDLLPLLHQAIASLTNAANFLNLIFQASELRETSVREDIEWYHALVRTMLEGDRPGLVRRAFLTFDADPTIQQPQLVLRASKGTTQDILLQDLTSAWTSFRPPAPDQSWFDIFKSSSPPLHALSKRVLLNDLSTLDTPKWARGDSYVINSSGVQWGEGPFLECVDGRFLPGWLLSLSMPFYGLKPDLSPEFRGVIRVDVNIQGFSVDQCATGDFWFANTHQCNRTSMECEPIPQKGFRMGQYCCRCKKGYYSPISDIQNKRNNRENNTQACYPEVPVCVPCWPGCAECEDGAPCWVQEDWLLRTAVLAVQGLFMILVLISMLVAYQCRKTKRIRSSGLLLLEMILCGSLLLYFPVFILYFKPSTFRCILLRWVRLLGFAIVYGTVALKMYRVLKVFLSRTAQRVPYITTTGVLRMLGVMVLTVSWFLCAWTVGVLQNRDRNVPLLIISTTSEGQGFNVCDLDRWDYMMAVAELLFLCWGSFLCSAVKTVPSAFHEPRYMSIAIHNELLLSSMFHLLRFARPSIHPDWMLLLFFTHTHVTITVTLGLLFVPKFLHMSRPGREEIAAEVYEDEVDLRRSCSYLNSSFTSNCWSDQSLDPDDIRDELKKLYSQLEVHKTKRMANSNPHLPKKRSSRLSIGRSIIKRISEIPESLSRQCSREDKDISAAIGTITRSGSYYKCHQTTSINTINETLMQPSPKLRKSHSAYDCTPEKEASLLNSSMKRSTEKRHSQHSDAGSINTTLLICKSASAHNLSLDTNLLLPEPTRFQKSLSVIERNDHRSVTPCRSIENVSISIKPTPAERPPKLTVDVDIKRQTSSALLSESFDKAEVCPWEVPEELPANKNLKHVTYSISTQEEPKSPGALSSPTLLYVCPWEYLPPPSPPAIETGNGIQSEVDSTKPKPPISCSAPGSPHTVSGKPKEFRVFSFRSTTQNLLAAKGVGELARSMSREKSLQESNVREGTLQKSQSTSMRLSPGSAASDKRTPQTQRRAMTTIGTKPSLVKQTAVRLPSNDSPERSPKHTAPVHVCPWESEEVILEVKKQNDTALQRQLSDKKELTVTASNDGLKPSELQLKSLLVVPNTEVCPWDVPKPFHQSSMSEVCPWEVVEVEQLQTKNMQTDVCPWETDSSRVTEDTICNTTVEVPMPGDIQKQVKVKPETSPSDVSDTNIPKVKQGSIKMSNETKPGERVIYVNTRECRLEQESAQKRGFMETSKTLKQEESDFPEPPPPVTDVCPWDLEPNTKDNSKPSMTKKATVCPWEAGDSEKSEERESATIKGSAKVDQKQESVYANVCPWETSESVKTMQKQDSISRNVCPWETQESVKTLQKQDSVRTNVCPWETEDGVKTLQKQDSVRTDVCPWEIEESVKTLQKQDSVRTNVCPWETEESVKTLPKQDSVRTNVCPWETEDQVKTLQKQDSVRTDVCPWETEEPVKTLQKQDSVRTNVCPWETEETVKSLQKQDSVCTNVCPWETSKSVKTIQKQDSVRTNVCPWETEEPVKTLQKQESVHAEVCPWETEEPEKALQKQDSVLSDVCPWEKNEPVKTLQKQDRVCSDVCPWESNTSAIQSVPQDSPRTVPTKSQESAQESVCPWETNMGTTATTEASTSQTVPDTIRTHPSDTQTAEELRVNPPLARRDALCPWEMEGGRQESITMHDNLDVFTWEETIPEEDDGDAETAAEAFIFPPDL

Target ORF information:

RefSeq Version	XM_017358296.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358296.2

ORF Insert Sequence:

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCTATTCC ACAAAAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGATTTGCCA GACCGTCAAT TCATCCTGAC TGGATGCTCT TGCTGTTCTT CACTCACACG 
CATGTCACTA TTACTGTTAC ACTCGGCCTG CTGTTTGTAC CCAAGTTTCT CCATATGTCA 
CGACCAGGCA GAGAAGAAAT AGCGGCAGAA GTATATGAGG ATGAGGTGGA CTTGCGACGT 
TCGTGTTCTT ACCTCAACAG TAGCTTCACC TCGAACTGCT GGAGTGACCA AAGTTTGGAC 
CCTGATGACA TTCGGGATGA GCTGAAGAAA CTGTATTCTC AGCTGGAAGT CCACAAGACC 
AAGAGGATGG CAAACAGCAA TCCCCACCTT CCAAAGAAGC GTAGCTCTCG TCTCAGTATT 
GGACGATCTA TTATAAAACG TATCTCTGAG ATCCCAGAAA GTTTGAGCAG ACAATGTAGC 
CGTGAAGACA AGGATATCAG TGCTGCAATA GGAACTATAA CACGATCGGG ATCTTATTAC 
AAATGTCATC AAACCACAAG TATTAACACT ATAAATGAAA CTTTAATGCA GCCTTCTCCA 
AAGCTGCGGA AGTCCCATAG TGCCTATGAT TGTACCCCAG AAAAGGAAGC CTCTCTATTA 
AACTCCTCCA TGAAGAGATC AACAGAAAAG AGGCACTCAC AGCATTCTGA TGCAGGATCT 
ATCAATACAA CACTGCTGAT CTGCAAGTCT GCCAGTGCCC ACAATCTATC GCTAGATACT 
AACCTTTTAC TTCCCGAGCC CACCAGGTTT CAAAAGTCAC TTAGTGTAAT AGAACGCAAT 
GACCATCGCT CTGTGACACC GTGTAGAAGC ATTGAAAATG TGTCAATTTC TATCAAACCT 
ACCCCAGCAG AAAGGCCACC CAAGCTGACT GTGGATGTTG ACATAAAAAG GCAGACTTCT 
AGTGCCTTGC TGTCAGAGTC CTTTGACAAG GCAGAGGTCT GCCCATGGGA GGTTCCAGAG 
GAGCTCCCTG CAAACAAAAA TCTAAAACAT GTCACATATT CAATCTCAAC ACAGGAAGAA 
CCAAAATCGC CTGGTGCTCT GTCATCACCA ACATTGTTAT ACGTTTGTCC GTGGGAATAC 
CTGCCACCTC CTTCTCCCCC TGCAATTGAA ACTGGGAATG GTATTCAGTC AGAAGTTGAT 
TCCACAAAAC CCAAACCTCC AATATCTTGT AGTGCTCCTG GGTCACCACA TACAGTTTCT 
GGCAAGCCAA AAGAATTTCG GGTTTTCTCT TTCCGCTCCA CTACACAAAA TCTCCTTGCA 
GCAAAGGGTG TTGGGGAACT TGCAAGAAGC ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT 
AACGTTAGGG AGGGAACACT ACAAAAATCT CAGTCTACAT CCATGAGATT ATCTCCAGGC 
AGTGCAGCTT CAGACAAACG TACCCCACAG ACACAAAGAC GGGCTATGAC TACTATAGGA 
ACAAAACCTT CCCTTGTGAA ACAAACAGCT GTACGGCTGC CATCAAATGA TTCTCCCGAA 
AGGAGCCCCA AGCATACTGC TCCAGTTCAC GTCTGCCCAT GGGAGTCAGA GGAGGTGATC 
TTGGAGGTCA AAAAGCAGAA TGATACAGCA TTACAAAGAC AACTGAGTGA CAAGAAAGAA 
TTGACTGTGA CAGCAAGTAA CGATGGTTTA AAGCCATCTG AGCTACAACT TAAGTCATTA 
TTAGTTGTTC CTAATACAGA AGTGTGTCCA TGGGATGTCC CAAAACCTTT TCATCAAAGT 
AGCATGTCTG AAGTCTGTCC TTGGGAGGTT GTGGAAGTCG AGCAATTGCA AACCAAAAAC 
ATGCAAACTG ATGTCTGTCC TTGGGAAACA GACTCATCAA GGGTTACTGA AGACACAATA 
TGCAATACCA CTGTTGAAGT ACCCATGCCT GGAGACATCC AGAAGCAGGT AAAAGTGAAG 
CCTGAAACAT CCCCTTCTGA TGTATCAGAT ACAAATATAC CCAAGGTCAA ACAAGGATCC 
ATAAAAATGT CCAATGAGAC AAAGCCTGGT GAAAGGGTTA TTTATGTTAA TACCAGAGAG 
TGTCGGTTGG AACAAGAATC GGCTCAGAAA AGAGGATTTA TGGAAACAAG TAAAACACTG 
AAGCAAGAAG AAAGTGATTT TCCTGAACCA CCCCCACCTG TAACTGATGT ATGTCCTTGG 
GATTTAGAGC CAAATACAAA AGACAATTCA AAACCCTCAA TGACTAAAAA AGCAACTGTT 
TGTCCGTGGG AGGCAGGGGA TTCTGAGAAG TCAGAAGAGA GGGAAAGTGC TACAATCAAG 
GGGTCAGCAA AAGTAGATCA AAAACAAGAA AGTGTATATG CAAATGTCTG TCCATGGGAA 
ACAAGCGAGT CTGTTAAGAC CATGCAGAAA CAAGATAGTA TCAGTAGAAA TGTCTGTCCA 
TGGGAGACAC AAGAGTCAGT GAAGACTTTG CAGAAACAAG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA CGGGGTAAAA ACCTTACAGA AACAAGATAG TGTCCGTACA 
GATGTTTGTC CATGGGAAAT AGAAGAATCT GTCAAGACCT TGCAGAAACA AGATAGTGTC 
CGTACAAATG TTTGTCCATG GGAAACAGAA GAATCTGTCA AGACCTTGCC GAAACAAGAT 
AGTGTCCGTA CAAATGTTTG TCCATGGGAG ACAGAAGACC AGGTAAAAAC CTTACAGAAA 
CAAGATAGTG TCCGTACAGA TGTTTGTCCA TGGGAGACAG AAGAGCCAGT CAAGACCTTG 
CAGAAACAGG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA GACGGTAAAG 
TCCTTGCAGA AGCAAGACAG TGTCTGTACA AATGTTTGTC CTTGGGAGAC AAGCAAGTCT 
GTTAAGACCA TACAGAAACA AGACAGTGTC CGTACAAATG TATGTCCATG GGAGACAGAA 
GAGCCAGTCA AGACCTTGCA GAAACAAGAA AGTGTCCATG CAGAAGTTTG TCCATGGGAG 
ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA CAAGACAGTG TTCTTTCAGA TGTTTGTCCA 
TGGGAGAAAA ACGAGCCAGT GAAGACCTTA CAGAAGCAAG ATCGTGTCTG TTCAGATGTC 
TGTCCATGGG AATCAAACAC ATCAGCGATT CAATCAGTGC CACAAGACTC CCCTCGTACG 
GTCCCAACAA AAAGTCAAGA AAGTGCTCAA GAATCTGTCT GTCCCTGGGA AACTAACATG 
GGAACAACAG CAACAACAGA AGCCAGCACA AGTCAAACTG TCCCAGACAC AATCAGAACA 
CACCCTTCAG ACACACAGAC TGCAGAAGAG TTACGGGTTA ACCCTCCTCT AGCTAGACGT 
GATGCATTAT GTCCCTGGGA AATGGAAGGG GGGCGACAAG AATCTATAAC CATGCATGAT 
AATTTAGATG TCTTCACTTG GGAGGAGACA ATCCCAGAGG AAGATGATGG TGATGCAGAA 
ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-156l18.8 ( XM_021479749.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335424.1 Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.

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CloneID	ODa50523
Clone ID Related Accession (Same CDS sequence)	XM_021479749.1
Accession Version	XM_021479749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCTATTCC ACAAAAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGATTTGCCA GACCGTCAAT TCATCCTGAC TGGATGCTCT TGCTGTTCTT CACTCACACG 
CATGTCACTA TTACTGTTAC ACTCGGCCTG CTGTTTGTAC CCAAGTTTCT CCATATGTCA 
CAACCAGGCA GAGAAGAAAT AGCGGCAGAG GTATATGAGG ATGAGGTGGA CTTGCGACGT 
TCGTGTTCTT ACCTCAACAG TAGCTTCACC TCGAACTGCT GGAGTGACCA AAGTTTGGAC 
CCTGATGACA TTCGGGATGA GCTGAAGAAA CTGTATTCTC AGCTGGAAGT CCACAAGACC 
AAGAGGATGG CAAACAGCAA TCCCCACCTT CCAAAGAAGC GTAGCTCTCG TCTCAGTATT 
GGACGATCTA TTATAAAACG TATCTCTGAG ATCCCAGAAA GTTTGAGCAG ACAATGTAGC 
CGTGAAGACA AGGATATCAG TGCTGCAATA GGAACTATAA CACGATCGGG ATCTTATTAC 
AAATGTCATC AAACCACAAG TATTAACACT ATAAATGAAA CTTTAATGCA GCCTTCTCCA 
AAGCTGCGGA AGTCCCATAG TGCCTATGAT TGTACCCCAG AAAAGGAAGC CTCTTTATTA 
AACTCCTCCA TGAAAAGATC AACAGAAAAG AGGCACTCAC AGCATTCTGA TGCAGGATCT 
ATCAATACAA CACTGCTGAT CTGCAAGTCT GCCAGTGCCC ACAATCTATC GCTAGATACT 
AACCTTTTAC TTCCCGAGCC CACCAGGTTT CAAAAGTCAC TTAGTGTAAT AGAACGCAAT 
GACCATCGCT CTGTGACACC GTGTAGAAGC ATTGAAAATG TGTCAATTTC TATCAAACCT 
ACCCCAGCAG AAAGGCCACC CAAGCTGACT GTGGATGTTG ACATAAAGAG GCAGACTTCT 
AGTGCCTTGC TGTCAGAGTC CTTTGACAAG GCAGAGGTCT GCCCATGGGA GGTTCCAGAG 
GAGCTCCCTG CAACCAAAAA TCTAAAACAT GTCACATATT CCATCTCAAC ACAGGAAGAA 
CCAAAATCGC CTGGTGCTCT GTCATCACCA ACATTGTTAT ACGTTTGTCC GTGGGAATAC 
CTGCCACCTC CTTCTCCCCC TGCAATTGAA ACTGGGAATG GTATTCAGTC AGAAGTTGAT 
TCCACAAAAC CCAAACCTCC AATATCTTGT AGTGCTCCTG GGTCACCACA TACAGTTTCT 
GGCAAGCCAA AAGAATTTCG GGTTTTCTCT TTCCGCTCCA CTACACAAAA TCTCCTTGCA 
GCAAAGGGTG TTGGGGAACT TGCAAGAAGC ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT 
AACGTTAGGG AGGGAACACT ACAAAAATCT CAGTCTACAT CCATGAGATT ATCTCCAGGC 
AGTGCAGCTT CAGACAAACG TACCCCACAG ACACAAAGAC GGGCTATGAC TACTATAGGA 
ACAAAACCTT CCCTTGTGAA ACAAACAGCT GTACGGCTGC CATCAAATGA TTCTCCCGAA 
AGGAGCCCCA AGCATACTGC TCCAGTTCAC GTCTGCCCAT GGGAGTCAGA GGAGGTGATC 
TTGGAGGTCA AAAAGCAGAA TGATATAGCA TTACAAAGAC AACTGAGTGA CAAGAAAGAA 
TTGACTGTGA CAGCAAGTAA CGATGGTTTA AAGCCATCTG AGCTACAACT TAAGTCATTA 
TTAGTTGTTC CTAATACAGA AGTGTGTCCA TGGGATGTCC CAAAACCTTT TCATCAAAGT 
AGCATGTCTG AAGTCTGTCC TTGGGAGGTT GTGGAAGTCG AGCAATTGCA AACCAAAAAC 
ATGCAAACTG ATGTCTGTCC TTGGGAAACA GACTCATCAA GGGTTACTGA AGACACAATA 
TGCAATACCA CTGTTGAAGT ACCCATGCCT GGAGACATCC AGAAGCAGGT AAAAGTGAAG 
CCTGAAACAT CCCCTTCTGA TGTATCAGAT ACAAATATAC CCAAGGTCAA ACAAGGATCC 
ATAAAAATGT CCAATGAGAC AAAGCCTGGT GAAAGGGTTA TTTATGTTAA TACCAGAGAG 
TGTCGGTTGG AACAAGAATC GGCTCAGAAA AGAGGATTTA TGGAAACAAG TAAAACACTG 
AAGCAAGAAG AAAGTGATTT TCCTGAACCA CCCCCACCTG TAACTGATGT ATGTCCTTGG 
GATTTAGAGC CAAATACAAA AGACAATTCA AAACCCTCAA TGACTAAAAA AGCAACTGTT 
TGTCCGTGGG AGGCAGGGGA TTCTGAAAAG TCAGAAGAGA GGGAAAGTGC TACAATCAAG 
GGGTCAGCAA AAGTACATCA AAAACAAGAA AGTGTATATG CAAATGTCTG TCCATGGGAA 
ACAAGCGAGT CTGTTAAGAC CATGCAGAAA CAAGATAGTA TCAGTAGAAA TGTCTGTCCA 
TGGGAGACAC AAGAGTCAGT GAAGACTTTG CAGAAACAAG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA CGGGGTAAAA ACCTTACAGA AACAAGATAG TGTCCGTACA 
GATGTTTGTC CATGGGAAAC AGAAGAATCT GTCAAGACCT TGCAGAAACA AGATAGTGTC 
CGTACAAATG TTTGTCCATG GGAAACAGAA GAATCTGTCA AGACCTTGCC GAAACAAGAT 
AGTGTCCGTA CAAATGTTTG TCCATGGGAG ACAGAAGACC AGGTAAAAAC CTTACAGAAA 
CAAGATAGTG TCCGTACAGA TGTTTGTCCA TGGGAGACAG AAGAGCCAGT CAAGACCTTG 
CAGAAACAGG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA GACGGTAAAG 
TCCTTGCAGA AGCAAGACAG TGTCTGTACA AATGTTTGTC CTTGGGAGAC AAGCAAGTCT 
GTTAAGACCA TACAGAAACA AGACAGTGTC CGTACAAATG TATGTCCATG GGAGACAGAA 
GAGCCAGTCA AGACCTTGCA GAAACAAGAA AGTGTCCATG CAGAAGTTTG TCCATGGGAG 
ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA CAAGACAGTG TTCTTTCAGA TGTTTGTCCA 
TGGGAGAAAA ATGAGCCAGT GAAGACCTTA CAGAAGCAAG ATCGTGTCTG TTCAGATGTC 
TGTCCATGGG AATCAAACAC ATCAGCGATT CAATCAGTGC CACAAGACTC CCCTCGTACG 
GTCCCAACAA AAAGTCAAGA AAGTGCTCAA GAATCTGTCT GTCCCTGGGA AACTAACATG 
GGAACAACAG CAACAACAGA AGCCAGCACA AGTCAAACTG TCCTAGACAC AATCAGAACA 
CACCCTTCAG ACACACAGAC TGCAGAAGAG TTACGGGTTA ACCCTCCTCT AGCTAGACGT 
GATGCATTAT GTCCCTGGGA AATGGAAGGG GGGCGACAAG AATCTATAAC CATGCATGAT 
AATTTAGATG TCTTCACTTG GGAGGAGACA ATCCCAGAGG AAGATGATGG TGATGCAGAA 
ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335424.1
CDS	1556..6934
Translation	MGPSAGVLPLLLLLPRLLCAQLSTVSELKDTSSLEFTPTPFLTDPNITAGKGLSPSPQATSLPPVTVEDRSAAESYLYNGDSSSLAASTCTRTFQLPVRRGPPPRDLLPLLHQAIASLTNAANFLNLIFQASELRETSVREDIEWYHALVRTMLEGDRPGLVRRAFLTFDADPTIQQPQLVLRASKGTTQDILLQDLTSAWTSFRPPAPDQSWFDIFKSSSPPLHALSKRVLLNDLSTLDTPKWARGDSYVINSSGVQWGEGPFLECVDGRFLPGWLLSLSMPFYGLKPDLSPEFRGVIRVDVNIQGFSVDQCATGDFWFANTHQCNRTSMECEPIPQKGFRMGQYCCRCKKGYYSPISDIQNKRNNRENNTQACYPEVPVCVPCWPGCAECEDGAPCWVQEDWLLRTAVLAVQGLFMILVLISMLVAYQCRKTKRIRSSGLLLLEMILCGSLLLYFPVFILYFKPSTFRCILLRWVRLLGFAIVYGTVALKMYRVLKVFLSRTAQRVPYITTTGVLRMLGVMVLTVSWFLCAWTVGVLQNRDRNVPLLIISTTSEGQGFNVCDLDRWDYMMAVAELLFLCWGSFLCSAVKTVPSAFHEPRYMSIAIHNELLLSSMFHLLRFARPSIHPDWMLLLFFTHTHVTITVTLGLLFVPKFLHMSQPGREEIAAEVYEDEVDLRRSCSYLNSSFTSNCWSDQSLDPDDIRDELKKLYSQLEVHKTKRMANSNPHLPKKRSSRLSIGRSIIKRISEIPESLSRQCSREDKDISAAIGTITRSGSYYKCHQTTSINTINETLMQPSPKLRKSHSAYDCTPEKEASLLNSSMKRSTEKRHSQHSDAGSINTTLLICKSASAHNLSLDTNLLLPEPTRFQKSLSVIERNDHRSVTPCRSIENVSISIKPTPAERPPKLTVDVDIKRQTSSALLSESFDKAEVCPWEVPEELPATKNLKHVTYSISTQEEPKSPGALSSPTLLYVCPWEYLPPPSPPAIETGNGIQSEVDSTKPKPPISCSAPGSPHTVSGKPKEFRVFSFRSTTQNLLAAKGVGELARSMSREKSLQESNVREGTLQKSQSTSMRLSPGSAASDKRTPQTQRRAMTTIGTKPSLVKQTAVRLPSNDSPERSPKHTAPVHVCPWESEEVILEVKKQNDIALQRQLSDKKELTVTASNDGLKPSELQLKSLLVVPNTEVCPWDVPKPFHQSSMSEVCPWEVVEVEQLQTKNMQTDVCPWETDSSRVTEDTICNTTVEVPMPGDIQKQVKVKPETSPSDVSDTNIPKVKQGSIKMSNETKPGERVIYVNTRECRLEQESAQKRGFMETSKTLKQEESDFPEPPPPVTDVCPWDLEPNTKDNSKPSMTKKATVCPWEAGDSEKSEERESATIKGSAKVHQKQESVYANVCPWETSESVKTMQKQDSISRNVCPWETQESVKTLQKQDSVRTNVCPWETEDGVKTLQKQDSVRTDVCPWETEESVKTLQKQDSVRTNVCPWETEESVKTLPKQDSVRTNVCPWETEDQVKTLQKQDSVRTDVCPWETEEPVKTLQKQDSVRTNVCPWETEETVKSLQKQDSVCTNVCPWETSKSVKTIQKQDSVRTNVCPWETEEPVKTLQKQESVHAEVCPWETEEPEKALQKQDSVLSDVCPWEKNEPVKTLQKQDRVCSDVCPWESNTSAIQSVPQDSPRTVPTKSQESAQESVCPWETNMGTTATTEASTSQTVLDTIRTHPSDTQTAEELRVNPPLARRDALCPWEMEGGRQESITMHDNLDVFTWEETIPEEDDGDAETAAEAFIFPPDL

Target ORF information:

RefSeq Version	XM_021479749.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479749.1

ORF Insert Sequence:

ATGGGCCCAT CTGCTGGTGT GCTACCACTA CTTCTGCTGC TGCCTAGACT ACTTTGTGCT 
CAGCTCAGCA CCGTGTCTGA ACTCAAGGAC ACATCCAGCT TGGAGTTCAC CCCAACACCT 
TTTCTGACTG ACCCAAATAT CACCGCAGGA AAAGGTTTGT CTCCTAGCCC CCAAGCCACC 
AGTCTACCTC CTGTAACAGT GGAGGACCGA TCCGCTGCAG AAAGCTACCT CTACAATGGC 
GATTCGTCCT CCTTGGCTGC CTCTACCTGC ACACGAACTT TCCAGCTGCC CGTTCGGCGA 
GGACCTCCAC CTCGAGACCT TCTCCCTCTT TTGCACCAAG CCATAGCATC TCTGACAAAT 
GCTGCCAACT TTCTCAACCT CATATTCCAA GCTAGTGAGC TGAGAGAAAC CAGTGTACGA 
GAGGATATAG AGTGGTACCA TGCACTAGTC CGGACTATGC TTGAAGGGGA TCGGCCAGGC 
TTGGTCAGAC GAGCTTTTCT AACATTCGAC GCCGACCCAA CAATCCAACA GCCCCAGTTG 
GTGCTCCGTG CCTCCAAAGG CACAACCCAA GACATTCTCC TGCAGGACCT CACATCTGCC 
TGGACCAGCT TCCGTCCTCC TGCCCCTGAC CAAAGCTGGT TTGATATATT CAAGTCTAGC 
TCTCCGCCCC TCCATGCCTT GTCTAAACGG GTTCTTCTAA ATGACCTAAG CACCCTAGAC 
ACTCCCAAGT GGGCACGTGG GGACAGTTAT GTGATCAATA GCAGTGGGGT TCAGTGGGGT 
GAAGGTCCTT TCCTTGAGTG TGTTGATGGC CGTTTTTTGC CAGGGTGGTT ACTTAGCCTT 
TCAATGCCAT TTTATGGGTT GAAGCCAGAC CTCAGCCCAG AGTTCAGGGG TGTGATTCGT 
GTCGACGTCA ACATCCAGGG ATTTAGCGTG GATCAATGTG CCACTGGAGA TTTCTGGTTC 
GCAAACACAC ACCAGTGTAA TCGGACCAGC ATGGAGTGTG AGCCTATTCC ACAAAAGGGC 
TTCCGGATGG GTCAGTACTG CTGCCGATGT AAAAAGGGCT ACTACAGTCC AATCTCAGAC 
ATACAGAACA AAAGGAACAA CCGTGAGAAT AACACCCAAG CTTGTTACCC AGAAGTCCCT 
GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT GAATGTGAGG ATGGAGCCCC GTGTTGGGTG 
CAGGAGGACT GGCTCCTGAG GACGGCTGTT CTGGCTGTGC AGGGCCTCTT CATGATCCTT 
GTGCTAATCA GCATGCTGGT GGCATATCAG TGCAGAAAAA CCAAGCGGAT CCGATCATCA 
GGGCTTTTGC TGCTGGAGAT GATCCTGTGT GGCTCTCTGT TGCTTTACTT CCCAGTTTTC 
ATTCTGTACT TCAAGCCCAG CACTTTCCGG TGTATTCTCC TCCGTTGGGT TCGTCTGCTT 
GGTTTTGCCA TTGTTTATGG AACTGTGGCA CTCAAGATGT ACCGGGTTCT AAAGGTGTTC 
CTGTCACGAA CTGCCCAGCG AGTACCATAC ATCACCACCA CTGGAGTGCT GAGGATGCTG 
GGAGTGATGG TGCTCACTGT CAGCTGGTTT CTGTGCGCAT GGACAGTGGG TGTCCTACAA 
AACAGAGACC GAAACGTCCC ACTTCTCATC ATATCCACTA CCTCAGAAGG ACAAGGCTTC 
AATGTGTGCG ATCTGGACCG ATGGGACTAC ATGATGGCAG TCGCGGAGCT GTTGTTCCTG 
TGCTGGGGGA GTTTTCTATG CAGCGCAGTG AAGACTGTAC CTTCTGCATT TCATGAACCA 
CGTTACATGA GCATCGCCAT CCACAATGAG CTACTGCTGT CCTCCATGTT TCATTTGCTC 
AGATTTGCCA GACCGTCAAT TCATCCTGAC TGGATGCTCT TGCTGTTCTT CACTCACACG 
CATGTCACTA TTACTGTTAC ACTCGGCCTG CTGTTTGTAC CCAAGTTTCT CCATATGTCA 
CAACCAGGCA GAGAAGAAAT AGCGGCAGAG GTATATGAGG ATGAGGTGGA CTTGCGACGT 
TCGTGTTCTT ACCTCAACAG TAGCTTCACC TCGAACTGCT GGAGTGACCA AAGTTTGGAC 
CCTGATGACA TTCGGGATGA GCTGAAGAAA CTGTATTCTC AGCTGGAAGT CCACAAGACC 
AAGAGGATGG CAAACAGCAA TCCCCACCTT CCAAAGAAGC GTAGCTCTCG TCTCAGTATT 
GGACGATCTA TTATAAAACG TATCTCTGAG ATCCCAGAAA GTTTGAGCAG ACAATGTAGC 
CGTGAAGACA AGGATATCAG TGCTGCAATA GGAACTATAA CACGATCGGG ATCTTATTAC 
AAATGTCATC AAACCACAAG TATTAACACT ATAAATGAAA CTTTAATGCA GCCTTCTCCA 
AAGCTGCGGA AGTCCCATAG TGCCTATGAT TGTACCCCAG AAAAGGAAGC CTCTTTATTA 
AACTCCTCCA TGAAAAGATC AACAGAAAAG AGGCACTCAC AGCATTCTGA TGCAGGATCT 
ATCAATACAA CACTGCTGAT CTGCAAGTCT GCCAGTGCCC ACAATCTATC GCTAGATACT 
AACCTTTTAC TTCCCGAGCC CACCAGGTTT CAAAAGTCAC TTAGTGTAAT AGAACGCAAT 
GACCATCGCT CTGTGACACC GTGTAGAAGC ATTGAAAATG TGTCAATTTC TATCAAACCT 
ACCCCAGCAG AAAGGCCACC CAAGCTGACT GTGGATGTTG ACATAAAGAG GCAGACTTCT 
AGTGCCTTGC TGTCAGAGTC CTTTGACAAG GCAGAGGTCT GCCCATGGGA GGTTCCAGAG 
GAGCTCCCTG CAACCAAAAA TCTAAAACAT GTCACATATT CCATCTCAAC ACAGGAAGAA 
CCAAAATCGC CTGGTGCTCT GTCATCACCA ACATTGTTAT ACGTTTGTCC GTGGGAATAC 
CTGCCACCTC CTTCTCCCCC TGCAATTGAA ACTGGGAATG GTATTCAGTC AGAAGTTGAT 
TCCACAAAAC CCAAACCTCC AATATCTTGT AGTGCTCCTG GGTCACCACA TACAGTTTCT 
GGCAAGCCAA AAGAATTTCG GGTTTTCTCT TTCCGCTCCA CTACACAAAA TCTCCTTGCA 
GCAAAGGGTG TTGGGGAACT TGCAAGAAGC ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT 
AACGTTAGGG AGGGAACACT ACAAAAATCT CAGTCTACAT CCATGAGATT ATCTCCAGGC 
AGTGCAGCTT CAGACAAACG TACCCCACAG ACACAAAGAC GGGCTATGAC TACTATAGGA 
ACAAAACCTT CCCTTGTGAA ACAAACAGCT GTACGGCTGC CATCAAATGA TTCTCCCGAA 
AGGAGCCCCA AGCATACTGC TCCAGTTCAC GTCTGCCCAT GGGAGTCAGA GGAGGTGATC 
TTGGAGGTCA AAAAGCAGAA TGATATAGCA TTACAAAGAC AACTGAGTGA CAAGAAAGAA 
TTGACTGTGA CAGCAAGTAA CGATGGTTTA AAGCCATCTG AGCTACAACT TAAGTCATTA 
TTAGTTGTTC CTAATACAGA AGTGTGTCCA TGGGATGTCC CAAAACCTTT TCATCAAAGT 
AGCATGTCTG AAGTCTGTCC TTGGGAGGTT GTGGAAGTCG AGCAATTGCA AACCAAAAAC 
ATGCAAACTG ATGTCTGTCC TTGGGAAACA GACTCATCAA GGGTTACTGA AGACACAATA 
TGCAATACCA CTGTTGAAGT ACCCATGCCT GGAGACATCC AGAAGCAGGT AAAAGTGAAG 
CCTGAAACAT CCCCTTCTGA TGTATCAGAT ACAAATATAC CCAAGGTCAA ACAAGGATCC 
ATAAAAATGT CCAATGAGAC AAAGCCTGGT GAAAGGGTTA TTTATGTTAA TACCAGAGAG 
TGTCGGTTGG AACAAGAATC GGCTCAGAAA AGAGGATTTA TGGAAACAAG TAAAACACTG 
AAGCAAGAAG AAAGTGATTT TCCTGAACCA CCCCCACCTG TAACTGATGT ATGTCCTTGG 
GATTTAGAGC CAAATACAAA AGACAATTCA AAACCCTCAA TGACTAAAAA AGCAACTGTT 
TGTCCGTGGG AGGCAGGGGA TTCTGAAAAG TCAGAAGAGA GGGAAAGTGC TACAATCAAG 
GGGTCAGCAA AAGTACATCA AAAACAAGAA AGTGTATATG CAAATGTCTG TCCATGGGAA 
ACAAGCGAGT CTGTTAAGAC CATGCAGAAA CAAGATAGTA TCAGTAGAAA TGTCTGTCCA 
TGGGAGACAC AAGAGTCAGT GAAGACTTTG CAGAAACAAG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA CGGGGTAAAA ACCTTACAGA AACAAGATAG TGTCCGTACA 
GATGTTTGTC CATGGGAAAC AGAAGAATCT GTCAAGACCT TGCAGAAACA AGATAGTGTC 
CGTACAAATG TTTGTCCATG GGAAACAGAA GAATCTGTCA AGACCTTGCC GAAACAAGAT 
AGTGTCCGTA CAAATGTTTG TCCATGGGAG ACAGAAGACC AGGTAAAAAC CTTACAGAAA 
CAAGATAGTG TCCGTACAGA TGTTTGTCCA TGGGAGACAG AAGAGCCAGT CAAGACCTTG 
CAGAAACAGG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA GACGGTAAAG 
TCCTTGCAGA AGCAAGACAG TGTCTGTACA AATGTTTGTC CTTGGGAGAC AAGCAAGTCT 
GTTAAGACCA TACAGAAACA AGACAGTGTC CGTACAAATG TATGTCCATG GGAGACAGAA 
GAGCCAGTCA AGACCTTGCA GAAACAAGAA AGTGTCCATG CAGAAGTTTG TCCATGGGAG 
ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA CAAGACAGTG TTCTTTCAGA TGTTTGTCCA 
TGGGAGAAAA ATGAGCCAGT GAAGACCTTA CAGAAGCAAG ATCGTGTCTG TTCAGATGTC 
TGTCCATGGG AATCAAACAC ATCAGCGATT CAATCAGTGC CACAAGACTC CCCTCGTACG 
GTCCCAACAA AAAGTCAAGA AAGTGCTCAA GAATCTGTCT GTCCCTGGGA AACTAACATG 
GGAACAACAG CAACAACAGA AGCCAGCACA AGTCAAACTG TCCTAGACAC AATCAGAACA 
CACCCTTCAG ACACACAGAC TGCAGAAGAG TTACGGGTTA ACCCTCCTCT AGCTAGACGT 
GATGCATTAT GTCCCTGGGA AATGGAAGGG GGGCGACAAG AATCTATAAC CATGCATGAT 
AATTTAGATG TCTTCACTTG GGAGGAGACA ATCCCAGAGG AAGATGATGG TGATGCAGAA 
ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-156l18.8 ( XM_017358296.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213785.1 Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), mRNA.

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CloneID	ODa54322
Clone ID Related Accession (Same CDS sequence)	XM_017358296.1
Accession Version	XM_017358296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable G-protein coupled receptor 179
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGTG AGCCTATTCC ACAAAAGGGC TTCCGGATGG GTCAGTACTG CTGCCGATGT 
AAAAAGGGCT ACTACAGTCC AATCTCAGAC ATACAGAACA AAAGGAACAA CCGTGAGAAT 
AACACCCAAG CTTGTTACCC AGAAGTCCCT GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT 
GAATGTGAGG ATGGAGCCCC GTGTTGGGTG CAGGAGGACT GGCTCCTGAG GACGGCTGTT 
CTGGCTGTGC AGGGCCTCTT CATGATCCTT GTGCTAATCA GCATGCTGGT GGCATATCAG 
TGCAGAAAAA CCAAGCGGAT CCGATCATCA GGGCTTTTGC TGCTGGAGAT GATCCTGTGT 
GGCTCTCTGT TGCTTTACTT CCCAGTTTTC ATTCTGTACT TCAAGCCCAG CACTTTCCGG 
TGTATTCTCC TCCGTTGGGT TCGTCTGCTT GGTTTTGCCA TTGTTTATGG AACTGTGGCA 
CTCAAGATGT ACCGGGTTCT AAAGGTGTTC CTGTCACGAA CTGCCCAGCG AGTACCATAC 
ATCACCACCA CTGGAGTGCT GAGGATGCTG GGAGTGATGG TGCTCACTGT CAGCTGGTTT 
CTGTGCGCAT GGACAGTGGG TGTCCTACAA AACAGAGACC GAAACGTCCC ACTTCTCATC 
ATATCCACTA CCTCAGAAGG ACAAGGCTTC AATGTGTGCG ATCTGGACCG ATGGGACTAC 
ATGATGGCAG TCGCGGAGCT GTTGTTCCTG TGCTGGGGGA GTTTTCTATG CAGCGCAGTG 
AAGACTGTAC CTTCTGCATT TCATGAACCA CGTTACATGA GCATCGCCAT CCACAATGAG 
CTACTGCTGT CCTCCATGTT TCATTTGCTC AGATTTGCCA GACCGTCAAT TCATCCTGAC 
TGGATGCTCT TGCTGTTCTT CACTCACACG CATGTCACTA TTACTGTTAC ACTCGGCCTG 
CTGTTTGTAC CCAAGTTTCT CCATATGTCA CAACCAGGCA GAGAAGAAAT AGCGGCAGAG 
GTATATGAGG ATGAGGTGGA CTTGCGACGT TCGTGTTCTT ACCTCAACAG TAGCTTCACC 
TCGAACTGCT GGAGTGACCA AAGTTTGGAC CCTGATGACA TTCGGGATGA GCTGAAGAAA 
CTGTATTCTC AGCTGGAAGT CCACAAGACC AAGAGGATGG CAAACAGCAA TCCCCACCTT 
CCAAAGAAGC GTAGCTCTCG TCTCAGTATT GGACGATCTA TTATAAAACG TATCTCTGAG 
ATCCCAGAAA GTTTGAGCAG ACAATGTAGC CGTGAAGACA AGGATATCAG TGCTGCAATA 
GGAACTATAA CACGATCGGG ATCTTATTAC AAATGTCATC AAACCACAAG TATTAACACT 
ATAAATGAAA CTTTAATGCA GCCTTCTCCA AAGCTGCGGA AGTCCCATAG TGCCTATGAT 
TGTACCCCAG AAAAGGAAGC CTCTTTATTA AACTCCTCCA TGAAAAGATC AACAGAAAAG 
AGGCACTCAC AGCATTCTGA TGCAGGATCT ATCAATACAA CACTGCTGAT CTGCAAGTCT 
GCCAGTGCCC ACAATCTATC GCTAGATACT AACCTTTTAC TTCCCGAGCC CACCAGGTTT 
CAAAAGTCAC TTAGTGTAAT AGAACGCAAT GACCATCGCT CTGTGACACC GTGTAGAAGC 
ATTGAAAATG TGTCAATTTC TATCAAACCT ACCCCAGCAG AAAGGCCACC CAAGCTGACT 
GTGGATGTTG ACATAAAGAG GCAGACTTCT AGTGCCTTGC TGTCAGAGTC CTTTGACAAG 
GCAGAGGTCT GCCCATGGGA GGTTCCAGAG GAGCTCCCTG CAACCAAAAA TCTAAAACAT 
GTCACATATT CCATCTCAAC ACAGGAAGAA CCAAAATCGC CTGGTGCTCT GTCATCACCA 
ACATTGTTAT ACGTTTGTCC GTGGGAATAC CTGCCACCTC CTTCTCCCCC TGCAATTGAA 
ACTGGGAATG GTATTCAGTC AGAAGTTGAT TCCACAAAAC CCAAACCTCC AATATCTTGT 
AGTGCTCCTG GGTCACCACA TACAGTTTCT GGCAAGCCAA AAGAATTTCG GGTTTTCTCT 
TTCCGCTCCA CTACACAAAA TCTCCTTGCA GCAAAGGGTG TTGGGGAACT TGCAAGAAGC 
ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT AACGTTAGGG AGGGAACACT ACAAAAATCT 
CAGTCTACAT CCATGAGATT ATCTCCAGGC AGTGCAGCTT CAGACAAACG TACCCCACAG 
ACACAAAGAC GGGCTATGAC TACTATAGGA ACAAAACCTT CCCTTGTGAA ACAAACAGCT 
GTACGGCTGC CATCAAATGA TTCTCCCGAA AGGAGCCCCA AGCATACTGC TCCAGTTCAC 
GTCTGCCCAT GGGAGTCAGA GGAGGTGATC TTGGAGGTCA AAAAGCAGAA TGATATAGCA 
TTACAAAGAC AACTGAGTGA CAAGAAAGAA TTGACTGTGA CAGCAAGTAA CGATGGTTTA 
AAGCCATCTG AGCTACAACT TAAGTCATTA TTAGTTGTTC CTAATACAGA AGTGTGTCCA 
TGGGATGTCC CAAAACCTTT TCATCAAAGT AGCATGTCTG AAGTCTGTCC TTGGGAGGTT 
GTGGAAGTCG AGCAATTGCA AACCAAAAAC ATGCAAACTG ATGTCTGTCC TTGGGAAACA 
GACTCATCAA GGGTTACTGA AGACACAATA TGCAATACCA CTGTTGAAGT ACCCATGCCT 
GGAGACATCC AGAAGCAGGT AAAAGTGAAG CCTGAAACAT CCCCTTCTGA TGTATCAGAT 
ACAAATATAC CCAAGGTCAA ACAAGGATCC ATAAAAATGT CCAATGAGAC AAAGCCTGGT 
GAAAGGGTTA TTTATGTTAA TACCAGAGAG TGTCGGTTGG AACAAGAATC GGCTCAGAAA 
AGAGGATTTA TGGAAACAAG TAAAACACTG AAGCAAGAAG AAAGTGATTT TCCTGAACCA 
CCCCCACCTG TAACTGATGT ATGTCCTTGG GATTTAGAGC CAAATACAAA AGACAATTCA 
AAACCCTCAA TGACTAAAAA AGCAACTGTT TGTCCGTGGG AGGCAGGGGA TTCTGAAAAG 
TCAGAAGAGA GGGAAAGTGC TACAATCAAG GGGTCAGCAA AAGTACATCA AAAACAAGAA 
AGTGTATATG CAAATGTCTG TCCATGGGAA ACAAGCGAGT CTGTTAAGAC CATGCAGAAA 
CAAGATAGTA TCAGTAGAAA TGTCTGTCCA TGGGAGACAC AAGAGTCAGT GAAGACTTTG 
CAGAAACAAG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA CGGGGTAAAA 
ACCTTACAGA AACAAGATAG TGTCCGTACA GATGTTTGTC CATGGGAAAC AGAAGAATCT 
GTCAAGACCT TGCAGAAACA AGATAGTGTC CGTACAAATG TTTGTCCATG GGAAACAGAA 
GAATCTGTCA AGACCTTGCC GAAACAAGAT AGTGTCCGTA CAAATGTTTG TCCATGGGAG 
ACAGAAGACC AGGTAAAAAC CTTACAGAAA CAAGATAGTG TCCGTACAGA TGTTTGTCCA 
TGGGAGACAG AAGAGCCAGT CAAGACCTTG CAGAAACAGG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA GACGGTAAAG TCCTTGCAGA AGCAAGACAG TGTCTGTACA 
AATGTTTGTC CTTGGGAGAC AAGCAAGTCT GTTAAGACCA TACAGAAACA AGACAGTGTC 
CGTACAAATG TATGTCCATG GGAGACAGAA GAGCCAGTCA AGACCTTGCA GAAACAAGAA 
AGTGTCCATG CAGAAGTTTG TCCATGGGAG ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA 
CAAGACAGTG TTCTTTCAGA TGTTTGTCCA TGGGAGAAAA ATGAGCCAGT GAAGACCTTA 
CAGAAGCAAG ATCGTGTCTG TTCAGATGTC TGTCCATGGG AATCAAACAC ATCAGCGATT 
CAATCAGTGC CACAAGACTC CCCTCGTACG GTCCCAACAA AAAGTCAAGA AAGTGCTCAA 
GAATCTGTCT GTCCCTGGGA AACTAACATG GGAACAACAG CAACAACAGA AGCCAGCACA 
AGTCAAACTG TCCTAGACAC AATCAGAACA CACCCTTCAG ACACACAGAC TGCAGAAGAG 
TTACGGGTTA ACCCTCCTCT AGCTAGACGT GATGCATTAT GTCCCTGGGA AATGGAAGGG 
GGGCGACAAG AATCTATAAC CATGCATGAT AATTTAGATG TCTTCACTTG GGAGGAGACA 
ATCCCAGAGG AAGATGATGG TGATGCAGAA ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA 
GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213785.1
CDS	98..4486
Translation	MECEPIPQKGFRMGQYCCRCKKGYYSPISDIQNKRNNRENNTQACYPEVPVCVPCWPGCAECEDGAPCWVQEDWLLRTAVLAVQGLFMILVLISMLVAYQCRKTKRIRSSGLLLLEMILCGSLLLYFPVFILYFKPSTFRCILLRWVRLLGFAIVYGTVALKMYRVLKVFLSRTAQRVPYITTTGVLRMLGVMVLTVSWFLCAWTVGVLQNRDRNVPLLIISTTSEGQGFNVCDLDRWDYMMAVAELLFLCWGSFLCSAVKTVPSAFHEPRYMSIAIHNELLLSSMFHLLRFARPSIHPDWMLLLFFTHTHVTITVTLGLLFVPKFLHMSQPGREEIAAEVYEDEVDLRRSCSYLNSSFTSNCWSDQSLDPDDIRDELKKLYSQLEVHKTKRMANSNPHLPKKRSSRLSIGRSIIKRISEIPESLSRQCSREDKDISAAIGTITRSGSYYKCHQTTSINTINETLMQPSPKLRKSHSAYDCTPEKEASLLNSSMKRSTEKRHSQHSDAGSINTTLLICKSASAHNLSLDTNLLLPEPTRFQKSLSVIERNDHRSVTPCRSIENVSISIKPTPAERPPKLTVDVDIKRQTSSALLSESFDKAEVCPWEVPEELPATKNLKHVTYSISTQEEPKSPGALSSPTLLYVCPWEYLPPPSPPAIETGNGIQSEVDSTKPKPPISCSAPGSPHTVSGKPKEFRVFSFRSTTQNLLAAKGVGELARSMSREKSLQESNVREGTLQKSQSTSMRLSPGSAASDKRTPQTQRRAMTTIGTKPSLVKQTAVRLPSNDSPERSPKHTAPVHVCPWESEEVILEVKKQNDIALQRQLSDKKELTVTASNDGLKPSELQLKSLLVVPNTEVCPWDVPKPFHQSSMSEVCPWEVVEVEQLQTKNMQTDVCPWETDSSRVTEDTICNTTVEVPMPGDIQKQVKVKPETSPSDVSDTNIPKVKQGSIKMSNETKPGERVIYVNTRECRLEQESAQKRGFMETSKTLKQEESDFPEPPPPVTDVCPWDLEPNTKDNSKPSMTKKATVCPWEAGDSEKSEERESATIKGSAKVHQKQESVYANVCPWETSESVKTMQKQDSISRNVCPWETQESVKTLQKQDSVRTNVCPWETEDGVKTLQKQDSVRTDVCPWETEESVKTLQKQDSVRTNVCPWETEESVKTLPKQDSVRTNVCPWETEDQVKTLQKQDSVRTDVCPWETEEPVKTLQKQDSVRTNVCPWETEETVKSLQKQDSVCTNVCPWETSKSVKTIQKQDSVRTNVCPWETEEPVKTLQKQESVHAEVCPWETEEPEKALQKQDSVLSDVCPWEKNEPVKTLQKQDRVCSDVCPWESNTSAIQSVPQDSPRTVPTKSQESAQESVCPWETNMGTTATTEASTSQTVLDTIRTHPSDTQTAEELRVNPPLARRDALCPWEMEGGRQESITMHDNLDVFTWEETIPEEDDGDAETAAEAFIFPPDL

Target ORF information:

RefSeq Version	XM_017358296.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-156l18.8 (si:ch211-156l18.8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358296.1

ORF Insert Sequence:

ATGGAGTGTG AGCCTATTCC ACAAAAGGGC TTCCGGATGG GTCAGTACTG CTGCCGATGT 
AAAAAGGGCT ACTACAGTCC AATCTCAGAC ATACAGAACA AAAGGAACAA CCGTGAGAAT 
AACACCCAAG CTTGTTACCC AGAAGTCCCT GTGTGTGTGC CCTGCTGGCC CGGCTGTGCT 
GAATGTGAGG ATGGAGCCCC GTGTTGGGTG CAGGAGGACT GGCTCCTGAG GACGGCTGTT 
CTGGCTGTGC AGGGCCTCTT CATGATCCTT GTGCTAATCA GCATGCTGGT GGCATATCAG 
TGCAGAAAAA CCAAGCGGAT CCGATCATCA GGGCTTTTGC TGCTGGAGAT GATCCTGTGT 
GGCTCTCTGT TGCTTTACTT CCCAGTTTTC ATTCTGTACT TCAAGCCCAG CACTTTCCGG 
TGTATTCTCC TCCGTTGGGT TCGTCTGCTT GGTTTTGCCA TTGTTTATGG AACTGTGGCA 
CTCAAGATGT ACCGGGTTCT AAAGGTGTTC CTGTCACGAA CTGCCCAGCG AGTACCATAC 
ATCACCACCA CTGGAGTGCT GAGGATGCTG GGAGTGATGG TGCTCACTGT CAGCTGGTTT 
CTGTGCGCAT GGACAGTGGG TGTCCTACAA AACAGAGACC GAAACGTCCC ACTTCTCATC 
ATATCCACTA CCTCAGAAGG ACAAGGCTTC AATGTGTGCG ATCTGGACCG ATGGGACTAC 
ATGATGGCAG TCGCGGAGCT GTTGTTCCTG TGCTGGGGGA GTTTTCTATG CAGCGCAGTG 
AAGACTGTAC CTTCTGCATT TCATGAACCA CGTTACATGA GCATCGCCAT CCACAATGAG 
CTACTGCTGT CCTCCATGTT TCATTTGCTC AGATTTGCCA GACCGTCAAT TCATCCTGAC 
TGGATGCTCT TGCTGTTCTT CACTCACACG CATGTCACTA TTACTGTTAC ACTCGGCCTG 
CTGTTTGTAC CCAAGTTTCT CCATATGTCA CAACCAGGCA GAGAAGAAAT AGCGGCAGAG 
GTATATGAGG ATGAGGTGGA CTTGCGACGT TCGTGTTCTT ACCTCAACAG TAGCTTCACC 
TCGAACTGCT GGAGTGACCA AAGTTTGGAC CCTGATGACA TTCGGGATGA GCTGAAGAAA 
CTGTATTCTC AGCTGGAAGT CCACAAGACC AAGAGGATGG CAAACAGCAA TCCCCACCTT 
CCAAAGAAGC GTAGCTCTCG TCTCAGTATT GGACGATCTA TTATAAAACG TATCTCTGAG 
ATCCCAGAAA GTTTGAGCAG ACAATGTAGC CGTGAAGACA AGGATATCAG TGCTGCAATA 
GGAACTATAA CACGATCGGG ATCTTATTAC AAATGTCATC AAACCACAAG TATTAACACT 
ATAAATGAAA CTTTAATGCA GCCTTCTCCA AAGCTGCGGA AGTCCCATAG TGCCTATGAT 
TGTACCCCAG AAAAGGAAGC CTCTTTATTA AACTCCTCCA TGAAAAGATC AACAGAAAAG 
AGGCACTCAC AGCATTCTGA TGCAGGATCT ATCAATACAA CACTGCTGAT CTGCAAGTCT 
GCCAGTGCCC ACAATCTATC GCTAGATACT AACCTTTTAC TTCCCGAGCC CACCAGGTTT 
CAAAAGTCAC TTAGTGTAAT AGAACGCAAT GACCATCGCT CTGTGACACC GTGTAGAAGC 
ATTGAAAATG TGTCAATTTC TATCAAACCT ACCCCAGCAG AAAGGCCACC CAAGCTGACT 
GTGGATGTTG ACATAAAGAG GCAGACTTCT AGTGCCTTGC TGTCAGAGTC CTTTGACAAG 
GCAGAGGTCT GCCCATGGGA GGTTCCAGAG GAGCTCCCTG CAACCAAAAA TCTAAAACAT 
GTCACATATT CCATCTCAAC ACAGGAAGAA CCAAAATCGC CTGGTGCTCT GTCATCACCA 
ACATTGTTAT ACGTTTGTCC GTGGGAATAC CTGCCACCTC CTTCTCCCCC TGCAATTGAA 
ACTGGGAATG GTATTCAGTC AGAAGTTGAT TCCACAAAAC CCAAACCTCC AATATCTTGT 
AGTGCTCCTG GGTCACCACA TACAGTTTCT GGCAAGCCAA AAGAATTTCG GGTTTTCTCT 
TTCCGCTCCA CTACACAAAA TCTCCTTGCA GCAAAGGGTG TTGGGGAACT TGCAAGAAGC 
ATGAGCAGAG AGAAAAGCCT ACAAGAAAGT AACGTTAGGG AGGGAACACT ACAAAAATCT 
CAGTCTACAT CCATGAGATT ATCTCCAGGC AGTGCAGCTT CAGACAAACG TACCCCACAG 
ACACAAAGAC GGGCTATGAC TACTATAGGA ACAAAACCTT CCCTTGTGAA ACAAACAGCT 
GTACGGCTGC CATCAAATGA TTCTCCCGAA AGGAGCCCCA AGCATACTGC TCCAGTTCAC 
GTCTGCCCAT GGGAGTCAGA GGAGGTGATC TTGGAGGTCA AAAAGCAGAA TGATATAGCA 
TTACAAAGAC AACTGAGTGA CAAGAAAGAA TTGACTGTGA CAGCAAGTAA CGATGGTTTA 
AAGCCATCTG AGCTACAACT TAAGTCATTA TTAGTTGTTC CTAATACAGA AGTGTGTCCA 
TGGGATGTCC CAAAACCTTT TCATCAAAGT AGCATGTCTG AAGTCTGTCC TTGGGAGGTT 
GTGGAAGTCG AGCAATTGCA AACCAAAAAC ATGCAAACTG ATGTCTGTCC TTGGGAAACA 
GACTCATCAA GGGTTACTGA AGACACAATA TGCAATACCA CTGTTGAAGT ACCCATGCCT 
GGAGACATCC AGAAGCAGGT AAAAGTGAAG CCTGAAACAT CCCCTTCTGA TGTATCAGAT 
ACAAATATAC CCAAGGTCAA ACAAGGATCC ATAAAAATGT CCAATGAGAC AAAGCCTGGT 
GAAAGGGTTA TTTATGTTAA TACCAGAGAG TGTCGGTTGG AACAAGAATC GGCTCAGAAA 
AGAGGATTTA TGGAAACAAG TAAAACACTG AAGCAAGAAG AAAGTGATTT TCCTGAACCA 
CCCCCACCTG TAACTGATGT ATGTCCTTGG GATTTAGAGC CAAATACAAA AGACAATTCA 
AAACCCTCAA TGACTAAAAA AGCAACTGTT TGTCCGTGGG AGGCAGGGGA TTCTGAAAAG 
TCAGAAGAGA GGGAAAGTGC TACAATCAAG GGGTCAGCAA AAGTACATCA AAAACAAGAA 
AGTGTATATG CAAATGTCTG TCCATGGGAA ACAAGCGAGT CTGTTAAGAC CATGCAGAAA 
CAAGATAGTA TCAGTAGAAA TGTCTGTCCA TGGGAGACAC AAGAGTCAGT GAAGACTTTG 
CAGAAACAAG ATAGTGTCCG TACAAATGTT TGTCCATGGG AGACAGAAGA CGGGGTAAAA 
ACCTTACAGA AACAAGATAG TGTCCGTACA GATGTTTGTC CATGGGAAAC AGAAGAATCT 
GTCAAGACCT TGCAGAAACA AGATAGTGTC CGTACAAATG TTTGTCCATG GGAAACAGAA 
GAATCTGTCA AGACCTTGCC GAAACAAGAT AGTGTCCGTA CAAATGTTTG TCCATGGGAG 
ACAGAAGACC AGGTAAAAAC CTTACAGAAA CAAGATAGTG TCCGTACAGA TGTTTGTCCA 
TGGGAGACAG AAGAGCCAGT CAAGACCTTG CAGAAACAGG ATAGTGTCCG TACAAATGTT 
TGTCCATGGG AGACAGAAGA GACGGTAAAG TCCTTGCAGA AGCAAGACAG TGTCTGTACA 
AATGTTTGTC CTTGGGAGAC AAGCAAGTCT GTTAAGACCA TACAGAAACA AGACAGTGTC 
CGTACAAATG TATGTCCATG GGAGACAGAA GAGCCAGTCA AGACCTTGCA GAAACAAGAA 
AGTGTCCATG CAGAAGTTTG TCCATGGGAG ACAGAAGAGC CTGAAAAGGC CTTGCAGAAA 
CAAGACAGTG TTCTTTCAGA TGTTTGTCCA TGGGAGAAAA ATGAGCCAGT GAAGACCTTA 
CAGAAGCAAG ATCGTGTCTG TTCAGATGTC TGTCCATGGG AATCAAACAC ATCAGCGATT 
CAATCAGTGC CACAAGACTC CCCTCGTACG GTCCCAACAA AAAGTCAAGA AAGTGCTCAA 
GAATCTGTCT GTCCCTGGGA AACTAACATG GGAACAACAG CAACAACAGA AGCCAGCACA 
AGTCAAACTG TCCTAGACAC AATCAGAACA CACCCTTCAG ACACACAGAC TGCAGAAGAG 
TTACGGGTTA ACCCTCCTCT AGCTAGACGT GATGCATTAT GTCCCTGGGA AATGGAAGGG 
GGGCGACAAG AATCTATAAC CATGCATGAT AATTTAGATG TCTTCACTTG GGAGGAGACA 
ATCCCAGAGG AAGATGATGG TGATGCAGAA ACTGCTGCAG AGGCCTTCAT CTTCCCTCCA 
GACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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si:ch211-156l18.8 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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