ano6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ano6
Entrez Gene ID	100535437
Full Name	anoctamin 6
Synonyms	si:ch73-177h5.1
General protein information	Preferred Names anoctamin 6 Names anoctamin-6 TMEM16F
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299990.1	XP_009298265.1	anoctamin-6 isoform X2
XM_009299990.2	XP_009298265.1	anoctamin-6 isoform X2
XM_005164462.3	XP_005164519.1	anoctamin-6 isoform X1
XM_005164462.4	XP_005164519.1	anoctamin-6 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ano6	NP_001240742.1
Bos taurus (cattle)	ANO6	XP_005206466.1
Homo sapiens (human)	ANO6	NP_001191732.1
Canis lupus familiaris (dog)	ANO6	XP_005637010.1
Pan troglodytes (chimpanzee)	ANO6	XP_003313797.1
	ANO6	XP_001092876.2
Rattus norvegicus (Norway rat)	Ano6	NP_001101578.1
Gallus gallus (chicken)	ANO6	XP_416045.2
Danio rerio (zebrafish)	LOC100535437	XP_003198261.1
Xenopus tropicalis (tropical clawed frog)	ano6	XP_002932853.1

Gene Ontology

The following ano6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09041	XM_005164462.4 Latest version!	Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa50183	XM_009299990.2 Latest version!	Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa50183	XM_009299990.1	Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa09041	XM_005164462.3	Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ano6 ( XM_005164462.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164519.1 Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09041
Clone ID Related Accession (Same CDS sequence)	XM_005164462.3 , XM_005164462.4
Accession Version	XM_005164462.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin-6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAA CTGAAGACAA TGACATTCTT GAGGACTCTG ATACAGATGG AGGAGACGTT 
GAGATGGCTG ATATGATCGA CTCTAAGACG GTGCAAACAT GCAGAACTAT GCCAAAAGGT 
TCAGATGTGC CAATAGTGGA TTTCAATGGG AACCAGGACT TTCTGTTTTT CAATGATGGA 
AAGCGAAGGA TCGACTTTGT TTTGGTGTAT GAATATGAAG AGTCTAAAAA TGGATCCAAC 
AAAAAAGCCT CAGTTATAAG AAGAAAAAAA AGGCGAAGAG AGTTTTTCGA AGGAAGCCTG 
ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164519.1
CDS	216..2948
Translation	MSETEDNDILEDSDTDGGDVEMADMIDSKTVQTCRTMPKGSDVPIVDFNGNQDFLFFNDGKRRIDFVLVYEYEESKNGSNKKASVIRRKKRRREFFEGSLMNKGLHLEMTQSVVDEKLFFVKLHMPFDVLCANAEVLHIKLPIKCNDLRTQSSKHNCCTKPFYPNEDVIGKEPDYFTAPFRKDLLNRFQLKEREGRELFTPTMRSRMVYYILSRAEYDIKGENKKFGITKLLAGGIYKAAYPLHDCRFNVKSKEEDCPNERYLLYSEWAHPKNFYKMQPLDLIRKYFGEKIGIYFAWLGFYTFMLSLAAAFGLGCFIYGYRSRESSTWSKEVCDPDIGGQIVMCPQCDNNCPYWRLNTTCESSKRLCVFDNFGTLVFAVFMSVWVTLFLEFWKRYQAKLEYKWDTVEFQQQEEQPRPEYEARCTYERFSPITQSNEKVPYTACGKCMRVSCGIGTVLFWILLIVASVVGVIVYRLAMFMVFSMQLRSDLNRKELEPFREYITPQMATSITASIINVIIIMILNIVYEKVAVWITDFELPRTKTEYENSLTLKMFLFQFVNFYSSCFYIAFFKGKIVGYPGEPVYWLGKFRNEECDPGGCLTELTTQLSIIMTGKAVWNNIQEVLLPWLKNLMFRHCTQVGSEKAIPRWEQDYQLQPMGTLGLFYEYLEMVIQFGFVTLFVASFPLAPLLALVNNLFEIRVDAWKFTTQFRRIVPEKAQDIGAWQPILQGVTILAVVTNTAIIAFTSDMIPRLVYYWAFSVSPYVENSNHTMAGFIDSSLSVFNISHFDDKTRPRDDIKPIWFNNITTCRYRDYRNPPDHQNPYQFNVYYWHVIAAKLAFMIVVEHIVYFTKFMLSYLIPDVPEALNEQIKRERFLTRDILNNSQLTDLIKPIREKLQTDVADFTELEELL

Target ORF information:

RefSeq Version	XM_005164462.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164462.3

ORF Insert Sequence:

ATGAGTGAAA CTGAAGACAA TGACATTCTT GAGGACTCTG ATACAGATGG AGGAGACGTT 
GAGATGGCTG ATATGATCGA CTCTAAGACG GTGCAAACAT GCAGAACTAT GCCAAAAGGT 
TCAGATGTGC CAATAGTGGA TTTCAATGGG AACCAGGACT TTCTGTTTTT CAATGATGGA 
AAGCGAAGGA TCGACTTTGT TTTGGTGTAT GAATATGAAG AGTCTAAAAA TGGATCCAAC 
AAAAAAGCCT CAGTTATAAG AAGAAAAAAA AGGCGAAGAG AGTTTTTCGA AGGAAGCCTG 
ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano6 ( XM_005164462.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164519.1 Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09041
Clone ID Related Accession (Same CDS sequence)	XM_005164462.3 , XM_005164462.4
Accession Version	XM_005164462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin-6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAA CTGAAGACAA TGACATTCTT GAGGACTCTG ATACAGATGG AGGAGACGTT 
GAGATGGCTG ATATGATCGA CTCTAAGACG GTGCAAACAT GCAGAACTAT GCCAAAAGGT 
TCAGATGTGC CAATAGTGGA TTTCAATGGG AACCAGGACT TTCTGTTTTT CAATGATGGA 
AAGCGAAGGA TCGACTTTGT TTTGGTGTAT GAATATGAAG AGTCTAAAAA TGGATCCAAC 
AAAAAAGCCT CAGTTATAAG AAGAAAAAAA AGGCGAAGAG AGTTTTTCGA AGGAAGCCTG 
ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164519.1
CDS	312..3044
Translation	MSETEDNDILEDSDTDGGDVEMADMIDSKTVQTCRTMPKGSDVPIVDFNGNQDFLFFNDGKRRIDFVLVYEYEESKNGSNKKASVIRRKKRRREFFEGSLMNKGLHLEMTQSVVDEKLFFVKLHMPFDVLCANAEVLHIKLPIKCNDLRTQSSKHNCCTKPFYPNEDVIGKEPDYFTAPFRKDLLNRFQLKEREGRELFTPTMRSRMVYYILSRAEYDIKGENKKFGITKLLAGGIYKAAYPLHDCRFNVKSKEEDCPNERYLLYSEWAHPKNFYKMQPLDLIRKYFGEKIGIYFAWLGFYTFMLSLAAAFGLGCFIYGYRSRESSTWSKEVCDPDIGGQIVMCPQCDNNCPYWRLNTTCESSKRLCVFDNFGTLVFAVFMSVWVTLFLEFWKRYQAKLEYKWDTVEFQQQEEQPRPEYEARCTYERFSPITQSNEKVPYTACGKCMRVSCGIGTVLFWILLIVASVVGVIVYRLAMFMVFSMQLRSDLNRKELEPFREYITPQMATSITASIINVIIIMILNIVYEKVAVWITDFELPRTKTEYENSLTLKMFLFQFVNFYSSCFYIAFFKGKIVGYPGEPVYWLGKFRNEECDPGGCLTELTTQLSIIMTGKAVWNNIQEVLLPWLKNLMFRHCTQVGSEKAIPRWEQDYQLQPMGTLGLFYEYLEMVIQFGFVTLFVASFPLAPLLALVNNLFEIRVDAWKFTTQFRRIVPEKAQDIGAWQPILQGVTILAVVTNTAIIAFTSDMIPRLVYYWAFSVSPYVENSNHTMAGFIDSSLSVFNISHFDDKTRPRDDIKPIWFNNITTCRYRDYRNPPDHQNPYQFNVYYWHVIAAKLAFMIVVEHIVYFTKFMLSYLIPDVPEALNEQIKRERFLTRDILNNSQLTDLIKPIREKLQTDVADFTELEELL

Target ORF information:

RefSeq Version	XM_005164462.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 6 (ano6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164462.4

ORF Insert Sequence:

ATGAGTGAAA CTGAAGACAA TGACATTCTT GAGGACTCTG ATACAGATGG AGGAGACGTT 
GAGATGGCTG ATATGATCGA CTCTAAGACG GTGCAAACAT GCAGAACTAT GCCAAAAGGT 
TCAGATGTGC CAATAGTGGA TTTCAATGGG AACCAGGACT TTCTGTTTTT CAATGATGGA 
AAGCGAAGGA TCGACTTTGT TTTGGTGTAT GAATATGAAG AGTCTAAAAA TGGATCCAAC 
AAAAAAGCCT CAGTTATAAG AAGAAAAAAA AGGCGAAGAG AGTTTTTCGA AGGAAGCCTG 
ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano6 ( XM_009299990.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298265.1 Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50183
Clone ID Related Accession (Same CDS sequence)	XM_009299990.1 , XM_009299990.2
Accession Version	XM_009299990.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin-6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298265.1
CDS	246..2678
Translation	MNKGLHLEMTQSVVDEKLFFVKLHMPFDVLCANAEVLHIKLPIKCNDLRTQSSKHNCCTKPFYPNEDVIGKEPDYFTAPFRKDLLNRFQLKEREGRELFTPTMRSRMVYYILSRAEYDIKGENKKFGITKLLAGGIYKAAYPLHDCRFNVKSKEEDCPNERYLLYSEWAHPKNFYKMQPLDLIRKYFGEKIGIYFAWLGFYTFMLSLAAAFGLGCFIYGYRSRESSTWSKEVCDPDIGGQIVMCPQCDNNCPYWRLNTTCESSKRLCVFDNFGTLVFAVFMSVWVTLFLEFWKRYQAKLEYKWDTVEFQQQEEQPRPEYEARCTYERFSPITQSNEKVPYTACGKCMRVSCGIGTVLFWILLIVASVVGVIVYRLAMFMVFSMQLRSDLNRKELEPFREYITPQMATSITASIINVIIIMILNIVYEKVAVWITDFELPRTKTEYENSLTLKMFLFQFVNFYSSCFYIAFFKGKIVGYPGEPVYWLGKFRNEECDPGGCLTELTTQLSIIMTGKAVWNNIQEVLLPWLKNLMFRHCTQVGSEKAIPRWEQDYQLQPMGTLGLFYEYLEMVIQFGFVTLFVASFPLAPLLALVNNLFEIRVDAWKFTTQFRRIVPEKAQDIGAWQPILQGVTILAVVTNTAIIAFTSDMIPRLVYYWAFSVSPYVENSNHTMAGFIDSSLSVFNISHFDDKTRPRDDIKPIWFNNITTCRYRDYRNPPDHQNPYQFNVYYWHVIAAKLAFMIVVEHIVYFTKFMLSYLIPDVPEALNEQIKRERFLTRDILNNSQLTDLIKPIREKLQTDVADFTELEELL

Target ORF information:

RefSeq Version	XM_009299990.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299990.1

ORF Insert Sequence:

ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano6 ( XM_009299990.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298265.1 Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50183
Clone ID Related Accession (Same CDS sequence)	XM_009299990.1 , XM_009299990.2
Accession Version	XM_009299990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin-6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298265.1
CDS	246..2678
Translation	MNKGLHLEMTQSVVDEKLFFVKLHMPFDVLCANAEVLHIKLPIKCNDLRTQSSKHNCCTKPFYPNEDVIGKEPDYFTAPFRKDLLNRFQLKEREGRELFTPTMRSRMVYYILSRAEYDIKGENKKFGITKLLAGGIYKAAYPLHDCRFNVKSKEEDCPNERYLLYSEWAHPKNFYKMQPLDLIRKYFGEKIGIYFAWLGFYTFMLSLAAAFGLGCFIYGYRSRESSTWSKEVCDPDIGGQIVMCPQCDNNCPYWRLNTTCESSKRLCVFDNFGTLVFAVFMSVWVTLFLEFWKRYQAKLEYKWDTVEFQQQEEQPRPEYEARCTYERFSPITQSNEKVPYTACGKCMRVSCGIGTVLFWILLIVASVVGVIVYRLAMFMVFSMQLRSDLNRKELEPFREYITPQMATSITASIINVIIIMILNIVYEKVAVWITDFELPRTKTEYENSLTLKMFLFQFVNFYSSCFYIAFFKGKIVGYPGEPVYWLGKFRNEECDPGGCLTELTTQLSIIMTGKAVWNNIQEVLLPWLKNLMFRHCTQVGSEKAIPRWEQDYQLQPMGTLGLFYEYLEMVIQFGFVTLFVASFPLAPLLALVNNLFEIRVDAWKFTTQFRRIVPEKAQDIGAWQPILQGVTILAVVTNTAIIAFTSDMIPRLVYYWAFSVSPYVENSNHTMAGFIDSSLSVFNISHFDDKTRPRDDIKPIWFNNITTCRYRDYRNPPDHQNPYQFNVYYWHVIAAKLAFMIVVEHIVYFTKFMLSYLIPDVPEALNEQIKRERFLTRDILNNSQLTDLIKPIREKLQTDVADFTELEELL

Target ORF information:

RefSeq Version	XM_009299990.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 6 (ano6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299990.2

ORF Insert Sequence:

ATGAATAAGG GATTGCATCT GGAAATGACA CAATCCGTAG TGGATGAGAA GCTTTTCTTT 
GTGAAATTGC ACATGCCGTT CGATGTTCTG TGTGCAAATG CTGAAGTCCT GCATATTAAA 
CTGCCCATCA AATGCAATGA CCTCCGTACA CAATCCTCCA AGCACAACTG CTGCACTAAA 
CCATTCTACC CTAATGAGGA CGTGATTGGC AAAGAGCCGG ACTACTTCAC GGCGCCCTTC 
AGGAAAGATC TGCTCAACCG TTTTCAGCTA AAGGAAAGAG AGGGCAGGGA GTTATTTACA 
CCGACCATGA GGAGCAGAAT GGTGTATTAT ATCCTCAGCC GAGCAGAGTA TGATATAAAA 
GGAGAAAATA AGAAGTTTGG GATCACAAAG CTGCTTGCTG GAGGCATCTA TAAGGCTGCG 
TATCCATTAC ATGACTGCAG GTTTAATGTG AAGTCTAAAG AGGAGGACTG TCCCAATGAG 
CGTTATCTGC TGTACAGTGA GTGGGCGCAT CCTAAAAACT TCTACAAGAT GCAGCCGCTG 
GACCTGATCA GGAAGTATTT CGGTGAGAAA ATCGGCATTT ATTTCGCCTG GTTGGGTTTC 
TACACGTTCA TGTTGAGCCT CGCTGCTGCG TTCGGACTCG GCTGTTTCAT ATACGGGTAC 
AGAAGCAGAG AGAGCAGCAC ATGGAGCAAA GAGGTGTGTG ACCCTGATAT TGGTGGACAG 
ATTGTCATGT GCCCGCAGTG TGATAATAAC TGTCCGTATT GGCGACTCAA CACCACGTGC 
GAGTCCTCGA AAAGGCTGTG TGTGTTTGAT AACTTTGGGA CTTTAGTGTT TGCTGTCTTC 
ATGTCTGTTT GGGTGACTCT GTTCTTGGAG TTCTGGAAAC GTTACCAGGC CAAACTGGAG 
TACAAGTGGG ACACAGTGGA GTTTCAGCAG CAGGAGGAGC AGCCCAGGCC TGAATATGAG 
GCTAGATGCA CCTATGAACG ATTTAGTCCT ATCACACAGT CCAATGAGAA GGTGCCGTAT 
ACAGCCTGTG GAAAGTGCAT GAGAGTGTCG TGTGGCATTG GCACTGTGCT TTTCTGGATT 
CTGTTGATCG TAGCGTCAGT GGTCGGGGTG ATTGTGTATC GTTTGGCGAT GTTTATGGTG 
TTCTCGATGC AGCTGCGCTC TGACCTCAAT AGAAAAGAGC TGGAGCCGTT CAGAGAGTAC 
ATCACGCCTC AGATGGCCAC ATCCATCACC GCCTCCATCA TCAACGTCAT CATCATCATG 
ATCCTCAATA TTGTCTACGA GAAAGTGGCC GTCTGGATCA CAGACTTCGA GCTGCCACGG 
ACAAAAACAG AGTATGAGAA CAGCCTGACG CTCAAGATGT TCCTCTTTCA GTTCGTCAAT 
TTTTACTCGT CTTGTTTCTA CATCGCCTTC TTCAAAGGGA AGATTGTTGG TTATCCAGGA 
GAACCCGTCT ACTGGTTAGG AAAGTTTCGC AATGAGGAGT GTGATCCTGG TGGCTGTCTG 
ACTGAACTGA CCACTCAGCT GTCAATCATT ATGACTGGAA AGGCCGTCTG GAACAACATA 
CAGGAAGTCC TGCTGCCGTG GCTGAAGAAT CTGATGTTTC GTCATTGCAC CCAGGTGGGA 
TCAGAAAAAG CGATTCCTCG CTGGGAACAA GACTATCAGC TCCAGCCGAT GGGAACACTC 
GGCCTGTTTT ATGAATATCT GGAGATGGTG ATCCAGTTTG GCTTTGTGAC TCTGTTTGTG 
GCGTCTTTCC CGCTGGCTCC TCTTCTGGCG CTGGTCAATA ACCTGTTTGA GATCCGTGTG 
GACGCCTGGA AGTTCACCAC TCAGTTTCGT AGGATTGTCC CAGAGAAAGC TCAGGACATC 
GGAGCCTGGC AGCCCATCCT GCAGGGGGTG ACCATTCTGG CGGTGGTCAC TAATACTGCT 
ATCATCGCCT TCACTTCTGA CATGATCCCT CGTCTGGTTT ATTATTGGGC TTTTTCTGTC 
AGTCCTTACG TAGAGAACTC AAACCACACT ATGGCCGGCT TCATCGACAG CTCTCTGTCT 
GTTTTCAACA TTAGTCACTT TGACGATAAA ACCAGACCTC GAGACGACAT CAAACCGATC 
TGGTTTAACA ACATCACCAC GTGCCGGTAT CGAGATTACA GGAATCCCCC TGATCATCAG 
AACCCATATC AGTTCAATGT TTATTACTGG CATGTCATCG CCGCCAAACT GGCTTTCATG 
ATCGTGGTGG AGCACATTGT GTATTTCACC AAGTTCATGC TGTCCTACTT GATCCCGGAT 
GTTCCCGAAG CTCTGAACGA GCAGATCAAA CGTGAGCGAT TCCTGACGCG GGACATCCTA 
AACAACAGCC AGTTGACAGA CCTGATAAAA CCCATCCGAG AGAAACTGCA GACGGACGTG 
GCAGACTTCA CTGAGCTGGA GGAGCTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ano6 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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