RIMS1B cDNA ORF clone, Danio rerio(Zebrafish)

The following RIMS1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIMS1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa53893 XM_017356169.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa50145 XM_009292248.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50146 XM_009292253.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa50133 XM_009292225.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50135 XM_009292227.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50136 XM_009292228.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50137 XM_009292229.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50138 XM_009292230.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50139 XM_009292231.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50140 XM_009292234.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50141 XM_009292239.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50142 XM_009292243.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50143 XM_009292246.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa50144 XM_009292247.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa53893
    Clone ID Related Accession (Same CDS sequence) XM_017356169.1
    Accession Version XM_017356169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AACAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211658.1
    CDS1261..5568
    Translation

    Target ORF information:

    RefSeq Version XM_017356169.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AACAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50145
    Clone ID Related Accession (Same CDS sequence) XM_009292248.2
    Accession Version XM_009292248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGTGA TGTGGGTATG TAACCTGTGC AGGAAACAGC AGGAAATCCT AACTAAATCG 
    GGAGAATGGT TTTCATCAGG GCCCGGGGGG AGGCCTCTTA GTGTGGGTTC TCCCCTCAGT
    GGCCCTGAGT TGGAGAAGGA GAGGAAGTTG CGCTCCAGAT CCCAGGTTCC GGGTGCCAAC
    GACACTCTGC TACCCAACGC TGGTCCCGGT GACCATCCAA AGGGAGCAGA TACAATGCCC
    GCCTCACGCT CCCGCAGTGA ACCACCAAGA GAAAAGAGGA GGCCAGTGTC CATGCATGAG
    CAGAATGGAA AAGTGATTGG ACGTGGAGAG AGAAGAAGAG CTCCAGCCAG GTTATCGTCG
    CAGGCCTCTG AAGACCGAGC CCCAGGTGAG AATCGAGATG GTAGACGACT AGAGAAAGAC
    CACTCACAGG AGGACGATCC CACCCACCCT GAGCGACGAA GAAGACAGGA GGAGGAAGAG
    CGAGAGCGGC AAAGACGAGA GGAAGAGTAC CAGACCCGTT ACCGTAGTGA CCCCAATCTA
    GCCCGCTACC CTGTTAAACC GCAGAAGGAG GAACAAGAAA TGCGCATGCA TGCCAAAGTC
    TCCAAAATGC GCCAGGAGCG TCATCACAGT GACCTGGCCA TCAATGAAGT GGGCCTGATG
    CAGGATGGTG GCGAAGCTGC TGGAAATCGC ATTAGCAGGC AACGAGTAGC TAGCAATGAG
    GACCGCAAGG CTCTTTTAGA AAACCATCGA GCTTATTCTG TGGACAGGAC CGTGGGGGGT
    GGTGTTGGGG GGCAAGGGTC CCTCAAACAG GGCCATGGTG GCCCTCAAAT GGGCCTTACT
    GGGCCTCCAG ACCTCAGGGA TGGACAGGAT TGGGGCCCAA AGGGGCATCT GGAACCTGGG
    TCTGCAGCTT TTCTGCACAG GGCCAAGCGG GAAAAAGCTG CAGAAATCAT GCGTAAAGAT
    TCTTCTCTCA GCTCAGACCA GTCTGAGTCA TTACGGCCAC CTCCACCAAG AGCCTACAGA
    CCCAAACGAG GCCTCAACAA AAGGCAGATG TCCATCAGTA GCTCAGAAGA AGAAGGAGGT
    TCCACGCCTG AATACACCAG CTGTGAGGAT GTGGAGATCG AAAGTGTTAG TGAAAAAGGT
    GACTGGGACT GTCATCCACT GGACCCCACT GTGTGGCATC ATCCAGTGAG TTGGCAGCCA
    TCCAAAGAGG GAGATCATTT GATTGGCCGT ATCACTTTAA GTAAGCGATC CACTATGCCA
    CGAGAAGCCG GTTCTCTACT GGGTCTGAAG GTTGTTGGTG GGAAAATGAC AGAGTCTGGT
    AGACTGGGGG CCTTTATCAC CAAAGTAAAG AAAGGAAGTT TGGCTGACAT TGTCGGGCAT
    TTACGAGCAG GCGATGAGGT GTTGCAGTGG AATGGAAAAG CGTTGCCTGG AGCAACTAAA
    AAGGAAGTGT ATAATATTAT TCTGGAGTCT CAGTCTGCAC CTCAAGTGGA AATAGTTGTT
    TCCAGACCGA TTGGATGTAT TCAACGAATT CTATCCAAAA ACTTCTTGAA AAAGGTCTAT
    AGAGCATATC AAGAAACGCC AAGACTTCCA GGGACATCAC ACCCCCCTCT AGAATCAACT
    GGTTCGAGTT CTATAGATGA ATCTCAAAAG ATGGATCGTC CATCCATCTC TGTAATGTCT
    CCGACTAGTC CCGGGATGTT GAGAGACCTC CCTCTTGTAC TGCCAGGCCA GCTGTCGGTG
    AAATTATGGT ATGATAAAGT GGGCCATCAG CTTATTGTCA ATGTTCTACA AGCACGAGAA
    CTGCCCCCTC GACCTGATGG ACGACCCAGG AATCCCTACG TCAAAATGTA TTTTCTTCCT
    GATAGAAGTG ACAAAAGCAA AAGAAGGACA AAAACGGTGA AGAAGAGTGC AGAGCCAAAG
    TGGAACCAGA CGTTTCTGTA TTCTCACGTC CATCGCAGAG ACTTCAGAGA ACGCATGCTG
    GAGATCACAG TTTGGGATCA GCCCAGAGCC CAGGAAGAAG AGAGTGATTT TCTGGGAGAG
    ATTCTGATTG AGCTGGAAAC AGCTCTGTTG GATGATCAGG CACACTGGTA CAAATTTCAG
    ACCCATGACG TGTCATCGCT TCCGTTGCCC CAACCGTCCC CTTGCCTCCC GCGCAGACAT
    GTTCATGGAG ACAGTCCTAG TAAAAAACTG CAAAGATCTC ATCGCATCAT TGAAACAGAT
    TATGATGATG GTATAACAGT AGTGTCCTCA GCAGCAGAAA GGACCTCCAG AGACAGAGAG
    AGGTCAAGCA CATTGACTGT ACCTGAGAGA CAGGCGGCCA TACAGCATCG CTCGCGGTCA
    GTTTCTCCGC ACAGGGAAGA CCAGTGTAGA GCCCGCTCTC GACCCGCACA CATCCCTATG
    CAGAGGAGTC TGGATGAGAT CCACCAGAAC CGCCATCATT CCTACTCTCC CTCCCATTAC
    CACGACTCCC ACCAGGAACA TCGATCCGGA GACTCGGACT ATGAATATTC AGAGGACAGC
    GAGGTCCTGG AGATGCACAG ATCAATCCGA GGTGGGAGTG CTGAGTGCCT GCATACAAAC
    AGGAAATTAG CTAGACACTG TAACACACTT CCCCCAAAGA TGCCATTGTT AGTGAACGGT
    ATACATAAAG ATATTTACAG CTCTACCCTT CCTGCATGCT TAAAGAGCAA AGCACCACGA
    CGAACAGAGA GGGATGAAGT TGTCCCTCTT AGTGCCATTC CACAGCGCAT TCTCAGGTTC
    ACAGACGAGG TCAGCTCTGG TGATCTTCAG CCTTCATTAG ACAGAGTAAG AAGTGCCAGC
    ACAACTTGTT TGAGACCAGA GTCAAGTTTT CATTCACCTG AAAGTCATTT TCCATTTCAC
    AGGCATTCTG GCAATTTGGA TAGGCCAAGC TGTCAGATAG CTAACAAGAA AACCATTGAT
    GGTGACAGAC TTCAGCCCAC CTCTATCCTG AGAGGCAGCA GGAGGAGGAG AGAGACCCCC
    CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG GGTTCCTGCC CAAACTCTCC TCGAGCTGAC
    AGAGATCTTC CACACGGAGG ACATTATTCT CCAGCAGGGA CCACATCATT AGGGCGCAGG
    GGCCGACAGC TGCCACAACT CCCTGCAAAG AGCAGCAGTA TAGAACAAGG CCGTATACCT
    GAGGAGAAAC CTAACTCAGC TCTAGCAGTG GAGGAGCGGG CTCGTCAGTT GCAGATGAGA
    GTGCACTCGT ATCGGCCGGG ATCTTCTGCC AATTCTGCTC ATGATCCAGA GGCAGAGCTT
    AAGAACAGGA GAGATATGTA CAAGGACCAG CGGAGGAGCT GTGACAACAT GTCTGCGAGG
    TCATCAGACA GTGACATGAG CGACGTATCG GCCATCTCTC ATGCCAGCAG TGCCTCACGG
    CTGAGTGCCA CCAGCTACAT GTCCATTCAG TCTGAAAGGC CACACGGGAG AATCAGGTCA
    AAGTCATTGG AAAGTTGTAA AGGAGAAGAA AAGAAAGAAA GGAGGATTTC GTGGGGGGTG
    AATGGGACAG ACAACAGAAG GAGTTTGGGA AAGAGACGTT TCTCTGAGGA GCTTAAACGC
    AGACGGCACA CGGTGGCTGG AGATGTGAGA GCCATCTTTG CATACCTCAC CTCATACTTT
    CCATGCTTGA ATGCTGAGCC GGCACTGGAT CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT
    CAGCCTGTGG AAGAGAGACC CACCTCCCAG CTCCCAATCC CCACCCTTCG TCCCACAGAT
    AGAGAGTTAG GGTGTAGAGG TGATGGAGAA AGTGCAGTGG GACAGTCCTG CAGTACACTG
    ACTCCATCAG AGACAGATAC ACGACAGCTC CTTCAAACCT CCAGGGGACA GCAGAGGACA
    TGGCTGCGCT GCAATAGTGC ACTGCTGTGT CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290523.1
    CDS628..4584
    Translation

    Target ORF information:

    RefSeq Version XM_009292248.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGTGA TGTGGGTATG TAACCTGTGC AGGAAACAGC AGGAAATCCT AACTAAATCG 
    GGAGAATGGT TTTCATCAGG GCCCGGGGGG AGGCCTCTTA GTGTGGGTTC TCCCCTCAGT
    GGCCCTGAGT TGGAGAAGGA GAGGAAGTTG CGCTCCAGAT CCCAGGTTCC GGGTGCCAAC
    GACACTCTGC TACCCAACGC TGGTCCCGGT GACCATCCAA AGGGAGCAGA TACAATGCCC
    GCCTCACGCT CCCGCAGTGA ACCACCAAGA GAAAAGAGGA GGCCAGTGTC CATGCATGAG
    CAGAATGGAA AAGTGATTGG ACGTGGAGAG AGAAGAAGAG CTCCAGCCAG GTTATCGTCG
    CAGGCCTCTG AAGACCGAGC CCCAGGTGAG AATCGAGATG GTAGACGACT AGAGAAAGAC
    CACTCACAGG AGGACGATCC CACCCACCCT GAGCGACGAA GAAGACAGGA GGAGGAAGAG
    CGAGAGCGGC AAAGACGAGA GGAAGAGTAC CAGACCCGTT ACCGTAGTGA CCCCAATCTA
    GCCCGCTACC CTGTTAAACC GCAGAAGGAG GAACAAGAAA TGCGCATGCA TGCCAAAGTC
    TCCAAAATGC GCCAGGAGCG TCATCACAGT GACCTGGCCA TCAATGAAGT GGGCCTGATG
    CAGGATGGTG GCGAAGCTGC TGGAAATCGC ATTAGCAGGC AACGAGTAGC TAGCAATGAG
    GACCGCAAGG CTCTTTTAGA AAACCATCGA GCTTATTCTG TGGACAGGAC CGTGGGGGGT
    GGTGTTGGGG GGCAAGGGTC CCTCAAACAG GGCCATGGTG GCCCTCAAAT GGGCCTTACT
    GGGCCTCCAG ACCTCAGGGA TGGACAGGAT TGGGGCCCAA AGGGGCATCT GGAACCTGGG
    TCTGCAGCTT TTCTGCACAG GGCCAAGCGG GAAAAAGCTG CAGAAATCAT GCGTAAAGAT
    TCTTCTCTCA GCTCAGACCA GTCTGAGTCA TTACGGCCAC CTCCACCAAG AGCCTACAGA
    CCCAAACGAG GCCTCAACAA AAGGCAGATG TCCATCAGTA GCTCAGAAGA AGAAGGAGGT
    TCCACGCCTG AATACACCAG CTGTGAGGAT GTGGAGATCG AAAGTGTTAG TGAAAAAGGT
    GACTGGGACT GTCATCCACT GGACCCCACT GTGTGGCATC ATCCAGTGAG TTGGCAGCCA
    TCCAAAGAGG GAGATCATTT GATTGGCCGT ATCACTTTAA GTAAGCGATC CACTATGCCA
    CGAGAAGCCG GTTCTCTACT GGGTCTGAAG GTTGTTGGTG GGAAAATGAC AGAGTCTGGT
    AGACTGGGGG CCTTTATCAC CAAAGTAAAG AAAGGAAGTT TGGCTGACAT TGTCGGGCAT
    TTACGAGCAG GCGATGAGGT GTTGCAGTGG AATGGAAAAG CGTTGCCTGG AGCAACTAAA
    AAGGAAGTGT ATAATATTAT TCTGGAGTCT CAGTCTGCAC CTCAAGTGGA AATAGTTGTT
    TCCAGACCGA TTGGATGTAT TCAACGAATT CTATCCAAAA ACTTCTTGAA AAAGGTCTAT
    AGAGCATATC AAGAAACGCC AAGACTTCCA GGGACATCAC ACCCCCCTCT AGAATCAACT
    GGTTCGAGTT CTATAGATGA ATCTCAAAAG ATGGATCGTC CATCCATCTC TGTAATGTCT
    CCGACTAGTC CCGGGATGTT GAGAGACCTC CCTCTTGTAC TGCCAGGCCA GCTGTCGGTG
    AAATTATGGT ATGATAAAGT GGGCCATCAG CTTATTGTCA ATGTTCTACA AGCACGAGAA
    CTGCCCCCTC GACCTGATGG ACGACCCAGG AATCCCTACG TCAAAATGTA TTTTCTTCCT
    GATAGAAGTG ACAAAAGCAA AAGAAGGACA AAAACGGTGA AGAAGAGTGC AGAGCCAAAG
    TGGAACCAGA CGTTTCTGTA TTCTCACGTC CATCGCAGAG ACTTCAGAGA ACGCATGCTG
    GAGATCACAG TTTGGGATCA GCCCAGAGCC CAGGAAGAAG AGAGTGATTT TCTGGGAGAG
    ATTCTGATTG AGCTGGAAAC AGCTCTGTTG GATGATCAGG CACACTGGTA CAAATTTCAG
    ACCCATGACG TGTCATCGCT TCCGTTGCCC CAACCGTCCC CTTGCCTCCC GCGCAGACAT
    GTTCATGGAG ACAGTCCTAG TAAAAAACTG CAAAGATCTC ATCGCATCAT TGAAACAGAT
    TATGATGATG GTATAACAGT AGTGTCCTCA GCAGCAGAAA GGACCTCCAG AGACAGAGAG
    AGGTCAAGCA CATTGACTGT ACCTGAGAGA CAGGCGGCCA TACAGCATCG CTCGCGGTCA
    GTTTCTCCGC ACAGGGAAGA CCAGTGTAGA GCCCGCTCTC GACCCGCACA CATCCCTATG
    CAGAGGAGTC TGGATGAGAT CCACCAGAAC CGCCATCATT CCTACTCTCC CTCCCATTAC
    CACGACTCCC ACCAGGAACA TCGATCCGGA GACTCGGACT ATGAATATTC AGAGGACAGC
    GAGGTCCTGG AGATGCACAG ATCAATCCGA GGTGGGAGTG CTGAGTGCCT GCATACAAAC
    AGGAAATTAG CTAGACACTG TAACACACTT CCCCCAAAGA TGCCATTGTT AGTGAACGGT
    ATACATAAAG ATATTTACAG CTCTACCCTT CCTGCATGCT TAAAGAGCAA AGCACCACGA
    CGAACAGAGA GGGATGAAGT TGTCCCTCTT AGTGCCATTC CACAGCGCAT TCTCAGGTTC
    ACAGACGAGG TCAGCTCTGG TGATCTTCAG CCTTCATTAG ACAGAGTAAG AAGTGCCAGC
    ACAACTTGTT TGAGACCAGA GTCAAGTTTT CATTCACCTG AAAGTCATTT TCCATTTCAC
    AGGCATTCTG GCAATTTGGA TAGGCCAAGC TGTCAGATAG CTAACAAGAA AACCATTGAT
    GGTGACAGAC TTCAGCCCAC CTCTATCCTG AGAGGCAGCA GGAGGAGGAG AGAGACCCCC
    CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG GGTTCCTGCC CAAACTCTCC TCGAGCTGAC
    AGAGATCTTC CACACGGAGG ACATTATTCT CCAGCAGGGA CCACATCATT AGGGCGCAGG
    GGCCGACAGC TGCCACAACT CCCTGCAAAG AGCAGCAGTA TAGAACAAGG CCGTATACCT
    GAGGAGAAAC CTAACTCAGC TCTAGCAGTG GAGGAGCGGG CTCGTCAGTT GCAGATGAGA
    GTGCACTCGT ATCGGCCGGG ATCTTCTGCC AATTCTGCTC ATGATCCAGA GGCAGAGCTT
    AAGAACAGGA GAGATATGTA CAAGGACCAG CGGAGGAGCT GTGACAACAT GTCTGCGAGG
    TCATCAGACA GTGACATGAG CGACGTATCG GCCATCTCTC ATGCCAGCAG TGCCTCACGG
    CTGAGTGCCA CCAGCTACAT GTCCATTCAG TCTGAAAGGC CACACGGGAG AATCAGGTCA
    AAGTCATTGG AAAGTTGTAA AGGAGAAGAA AAGAAAGAAA GGAGGATTTC GTGGGGGGTG
    AATGGGACAG ACAACAGAAG GAGTTTGGGA AAGAGACGTT TCTCTGAGGA GCTTAAACGC
    AGACGGCACA CGGTGGCTGG AGATGTGAGA GCCATCTTTG CATACCTCAC CTCATACTTT
    CCATGCTTGA ATGCTGAGCC GGCACTGGAT CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT
    CAGCCTGTGG AAGAGAGACC CACCTCCCAG CTCCCAATCC CCACCCTTCG TCCCACAGAT
    AGAGAGTTAG GGTGTAGAGG TGATGGAGAA AGTGCAGTGG GACAGTCCTG CAGTACACTG
    ACTCCATCAG AGACAGATAC ACGACAGCTC CTTCAAACCT CCAGGGGACA GCAGAGGACA
    TGGCTGCGCT GCAATAGTGC ACTGCTGTGT CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50146
    Clone ID Related Accession (Same CDS sequence) XM_009292253.2
    Accession Version XM_009292253.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGGTAT GTAACCTGTG CAGGAAACAG CAGGAAATCC TAACTAAATC GGGAGAATGG 
    TTTTCATCAG GGCCCGGGGG GAGGCCTCTT AGTGTGGGTT CTCCCCTCAG TGGCCCTGAG
    TTGGAGAAGG AGAGGAAGTT GCGCTCCAGA TCCCAGGTTC CGGGTGCCAA CGACACTCTG
    CTACCCAACG CTGGTCCCGG TGACCATCCA AAGGGAGCAG ATACAATGCC CGCCTCACGC
    TCCCGCAGTG AACCACCAAG AGAAAAGAGG AGGCCAGTGT CCATGCATGA GCAGAATGGA
    AAAGTGATTG GACGTGGAGA GAGAAGAAGA GCTCCAGCCA GGTTATCGTC GCAGGCCTCT
    GAAGACCGAG CCCCAGGTGA GAATCGAGAT GGTAGACGAC TAGAGAAAGA CCACTCACAG
    GAGGACGATC CCACCCACCC TGAGCGACGA AGAAGACAGG AGGAGGAAGA GCGAGAGCGG
    CAAAGACGAG AGGAAGAGTA CCAGACCCGT TACCGTAGTG ACCCCAATCT AGCCCGCTAC
    CCTGTTAAAC CGCAGAAGGA GGAACAAGAA ATGCGCATGC ATGCCAAAGT CTCCAAAATG
    CGCCAGGAGC GTCATCACAG TGACCTGGCC ATCAATGAAG TGGGCCTGAT GCAGGATGGT
    GGCGAAGCTG CTGGAAATCG CATTAGCAGG CAACGAGTAG CTAGCAATGA GGACCGCAAG
    GCTCTTTTAG AAAACCATCG AGCTTATTCT GTGGACAGGA CCGTGGGGGG TGGTGTTGGG
    GGGCAAGGGT CCCTCAAACA GGGCCATGGT GGCCCTCAAA TGGGCCTTAC TGGGCCTCCA
    GACCTCAGGG ATGGACAGGA TTGGGGCCCA AAGGGGCATC TGGAACCTGG GTCTGCAGCT
    TTTCTGCACA GGGCCAAGCG GGAAAAAGCT GCAGAAATCA TGCGTAAAGA TTCTTCTCTC
    AGCTCAGACC AGTCTGAGTC ATTACGGCCA CCTCCACCAA GAGCCTACAG ACCCAAACGA
    GGCCTCAACA AAAGGCAGAT GTCCATCAGT AGCTCAGAAG AAGAAGGAGG TTCCACGCCT
    GAATACACCA GCTGTGAGGA TGTGGAGATC GAAAGTGTTA GTGAAAAAGG TGACTGGGAC
    TGTCATCCAC TGGACCCCAC TGTGTGGCAT CATCCAGTGA GTTGGCAGCC ATCCAAAGAG
    GGAGATCATT TGATTGGCCG TATCACTTTA AGTAAGCGAT CCACTATGCC ACGAGAAGCC
    GGTTCTCTAC TGGGTCTGAA GGTTGTTGGT GGGAAAATGA CAGAGTCTGG TAGACTGGGG
    GCCTTTATCA CCAAAGTAAA GAAAGGAAGT TTGGCTGACA TTGTCGGGCA TTTACGAGCA
    GGCGATGAGG TGTTGCAGTG GAATGGAAAA GCGTTGCCTG GAGCAACTAA AAAGGAAGTG
    TATAATATTA TTCTGGAGTC TCAGTCTGCA CCTCAAGTGG AAATAGTTGT TTCCAGACCG
    ATTGGATGTA TTCAACGAAT TCTATCCAAA AACTTCTTGA AAAAGGTCTA TAGAGCATAT
    CAAGAAACGC CAAGACTTCC AGGGACATCA CACCCCCCTC TAGAATCAAC TGGTTCGAGT
    TCTATAGATG AATCTCAAAA GATGGATCGT CCATCCATCT CTGTAATGTC TCCGACTAGT
    CCCGGGATGT TGAGAGACCT CCCTCTTGTA CTGCCAGGCC AGCTGTCGGT GAAATTATGG
    TATGATAAAG TGGGCCATCA GCTTATTGTC AATGTTCTAC AAGCACGAGA ACTGCCCCCT
    CGACCTGATG GACGACCCAG GAATCCCTAC GTCAAAATGT ATTTTCTTCC TGATAGAAGT
    GACAAAAGCA AAAGAAGGAC AAAAACGGTG AAGAAGAGTG CAGAGCCAAA GTGGAACCAG
    ACGTTTCTGT ATTCTCACGT CCATCGCAGA GACTTCAGAG AACGCATGCT GGAGATCACA
    GTTTGGGATC AGCCCAGAGC CCAGGAAGAA GAGAGTGATT TTCTGGGAGA GATTCTGATT
    GAGCTGGAAA CAGCTCTGTT GGATGATCAG GCACACTGGT ACAAATTTCA GACCCATGAC
    GTGTCATCGC TTCCGTTGCC CCAACCGTCC CCTTGCCTCC CGCGCAGACA TGTTCATGGA
    GACAGTCCTA GTAAAAAACT GCAAAGATCT CATCGCATCA TTGAAACAGA TTATGATGAT
    GGTATAACAG TAGTGTCCTC AGCAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290528.1
    CDS619..4566
    Translation

    Target ORF information:

    RefSeq Version XM_009292253.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292253.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGGTAT GTAACCTGTG CAGGAAACAG CAGGAAATCC TAACTAAATC GGGAGAATGG 
    TTTTCATCAG GGCCCGGGGG GAGGCCTCTT AGTGTGGGTT CTCCCCTCAG TGGCCCTGAG
    TTGGAGAAGG AGAGGAAGTT GCGCTCCAGA TCCCAGGTTC CGGGTGCCAA CGACACTCTG
    CTACCCAACG CTGGTCCCGG TGACCATCCA AAGGGAGCAG ATACAATGCC CGCCTCACGC
    TCCCGCAGTG AACCACCAAG AGAAAAGAGG AGGCCAGTGT CCATGCATGA GCAGAATGGA
    AAAGTGATTG GACGTGGAGA GAGAAGAAGA GCTCCAGCCA GGTTATCGTC GCAGGCCTCT
    GAAGACCGAG CCCCAGGTGA GAATCGAGAT GGTAGACGAC TAGAGAAAGA CCACTCACAG
    GAGGACGATC CCACCCACCC TGAGCGACGA AGAAGACAGG AGGAGGAAGA GCGAGAGCGG
    CAAAGACGAG AGGAAGAGTA CCAGACCCGT TACCGTAGTG ACCCCAATCT AGCCCGCTAC
    CCTGTTAAAC CGCAGAAGGA GGAACAAGAA ATGCGCATGC ATGCCAAAGT CTCCAAAATG
    CGCCAGGAGC GTCATCACAG TGACCTGGCC ATCAATGAAG TGGGCCTGAT GCAGGATGGT
    GGCGAAGCTG CTGGAAATCG CATTAGCAGG CAACGAGTAG CTAGCAATGA GGACCGCAAG
    GCTCTTTTAG AAAACCATCG AGCTTATTCT GTGGACAGGA CCGTGGGGGG TGGTGTTGGG
    GGGCAAGGGT CCCTCAAACA GGGCCATGGT GGCCCTCAAA TGGGCCTTAC TGGGCCTCCA
    GACCTCAGGG ATGGACAGGA TTGGGGCCCA AAGGGGCATC TGGAACCTGG GTCTGCAGCT
    TTTCTGCACA GGGCCAAGCG GGAAAAAGCT GCAGAAATCA TGCGTAAAGA TTCTTCTCTC
    AGCTCAGACC AGTCTGAGTC ATTACGGCCA CCTCCACCAA GAGCCTACAG ACCCAAACGA
    GGCCTCAACA AAAGGCAGAT GTCCATCAGT AGCTCAGAAG AAGAAGGAGG TTCCACGCCT
    GAATACACCA GCTGTGAGGA TGTGGAGATC GAAAGTGTTA GTGAAAAAGG TGACTGGGAC
    TGTCATCCAC TGGACCCCAC TGTGTGGCAT CATCCAGTGA GTTGGCAGCC ATCCAAAGAG
    GGAGATCATT TGATTGGCCG TATCACTTTA AGTAAGCGAT CCACTATGCC ACGAGAAGCC
    GGTTCTCTAC TGGGTCTGAA GGTTGTTGGT GGGAAAATGA CAGAGTCTGG TAGACTGGGG
    GCCTTTATCA CCAAAGTAAA GAAAGGAAGT TTGGCTGACA TTGTCGGGCA TTTACGAGCA
    GGCGATGAGG TGTTGCAGTG GAATGGAAAA GCGTTGCCTG GAGCAACTAA AAAGGAAGTG
    TATAATATTA TTCTGGAGTC TCAGTCTGCA CCTCAAGTGG AAATAGTTGT TTCCAGACCG
    ATTGGATGTA TTCAACGAAT TCTATCCAAA AACTTCTTGA AAAAGGTCTA TAGAGCATAT
    CAAGAAACGC CAAGACTTCC AGGGACATCA CACCCCCCTC TAGAATCAAC TGGTTCGAGT
    TCTATAGATG AATCTCAAAA GATGGATCGT CCATCCATCT CTGTAATGTC TCCGACTAGT
    CCCGGGATGT TGAGAGACCT CCCTCTTGTA CTGCCAGGCC AGCTGTCGGT GAAATTATGG
    TATGATAAAG TGGGCCATCA GCTTATTGTC AATGTTCTAC AAGCACGAGA ACTGCCCCCT
    CGACCTGATG GACGACCCAG GAATCCCTAC GTCAAAATGT ATTTTCTTCC TGATAGAAGT
    GACAAAAGCA AAAGAAGGAC AAAAACGGTG AAGAAGAGTG CAGAGCCAAA GTGGAACCAG
    ACGTTTCTGT ATTCTCACGT CCATCGCAGA GACTTCAGAG AACGCATGCT GGAGATCACA
    GTTTGGGATC AGCCCAGAGC CCAGGAAGAA GAGAGTGATT TTCTGGGAGA GATTCTGATT
    GAGCTGGAAA CAGCTCTGTT GGATGATCAG GCACACTGGT ACAAATTTCA GACCCATGAC
    GTGTCATCGC TTCCGTTGCC CCAACCGTCC CCTTGCCTCC CGCGCAGACA TGTTCATGGA
    GACAGTCCTA GTAAAAAACT GCAAAGATCT CATCGCATCA TTGAAACAGA TTATGATGAT
    GGTATAACAG TAGTGTCCTC AGCAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50133
    Clone ID Related Accession (Same CDS sequence) XM_009292225.2
    Accession Version XM_009292225.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGGCC GTATACCTGA GGAGAAACCT
    AACTCAGCTC TAGCAGTGGA GGAGCGGGCT CGTCAGTTGC AGATGAGAGT GCACTCGTAT
    CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT GATCCAGAGG CAGAGCTTAA GAACAGGAGA
    GATATGTACA AGGACCAGCG GAGGAGCTGT GACAACATGT CTGCGAGGTC ATCAGACAGT
    GACATGAGCG ACGTATCGGC CATCTCTCAT GCCAGCAGTG CCTCACGGCT GAGTGCCACC
    AGCTACATGT CCATTCAGTC TGAAAGGCCA CACGGGAGAA TCAGGTCAAA GTCATTGGAA
    AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG AGGATTTCGT GGGGGGTGAA TGGGACAGAC
    AACAGAAGGA GTTTGGGAAA GAGACGTTTC TCTGAGGAGC TTAAACGCAG ACGGCACACG
    GTGGCTGGAG ATGTGAGAGC CATCTTTGCA TACCTCACCT CATACTTTCC ATGCTTGAAT
    GCTGAGCCGG CACTGGATCC CCCCAAACCC AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA
    GAGAGACCCA CCTCCCAGCT CCCAATCCCC ACCCTTCGTC CCACAGATAG AGAGTTAGGG
    TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA CAGTCCTGCA GTACACTGAC TCCATCAGAG
    ACAGATACAC GACAGCTCCT TCAAACCTCC AGGGGACAGC AGAGGACATG GCTGCGCTGC
    AATAGTGCAC TGCTGTGTCA TAATACAGAC TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290500.1
    CDS1261..5625
    Translation

    Target ORF information:

    RefSeq Version XM_009292225.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292225.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGGCC GTATACCTGA GGAGAAACCT
    AACTCAGCTC TAGCAGTGGA GGAGCGGGCT CGTCAGTTGC AGATGAGAGT GCACTCGTAT
    CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT GATCCAGAGG CAGAGCTTAA GAACAGGAGA
    GATATGTACA AGGACCAGCG GAGGAGCTGT GACAACATGT CTGCGAGGTC ATCAGACAGT
    GACATGAGCG ACGTATCGGC CATCTCTCAT GCCAGCAGTG CCTCACGGCT GAGTGCCACC
    AGCTACATGT CCATTCAGTC TGAAAGGCCA CACGGGAGAA TCAGGTCAAA GTCATTGGAA
    AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG AGGATTTCGT GGGGGGTGAA TGGGACAGAC
    AACAGAAGGA GTTTGGGAAA GAGACGTTTC TCTGAGGAGC TTAAACGCAG ACGGCACACG
    GTGGCTGGAG ATGTGAGAGC CATCTTTGCA TACCTCACCT CATACTTTCC ATGCTTGAAT
    GCTGAGCCGG CACTGGATCC CCCCAAACCC AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA
    GAGAGACCCA CCTCCCAGCT CCCAATCCCC ACCCTTCGTC CCACAGATAG AGAGTTAGGG
    TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA CAGTCCTGCA GTACACTGAC TCCATCAGAG
    ACAGATACAC GACAGCTCCT TCAAACCTCC AGGGGACAGC AGAGGACATG GCTGCGCTGC
    AATAGTGCAC TGCTGTGTCA TAATACAGAC TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50135
    Clone ID Related Accession (Same CDS sequence) XM_009292227.2
    Accession Version XM_009292227.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGGCATTCTG GCAATTTGGA TAGGCCAAGC
    TGTCAGATAG CTAACAAGAA AACCATTGAT GGTGACAGAC TTCAGCCCAC CTCTATCCTG
    AGAGGCAGCA GGAGGAGGAG AGAGACCCCC CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG
    GGTTCCTGCC CAAACTCTCC TCGAGCTGAC AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290502.1
    CDS1261..5607
    Translation

    Target ORF information:

    RefSeq Version XM_009292227.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGGCATTCTG GCAATTTGGA TAGGCCAAGC
    TGTCAGATAG CTAACAAGAA AACCATTGAT GGTGACAGAC TTCAGCCCAC CTCTATCCTG
    AGAGGCAGCA GGAGGAGGAG AGAGACCCCC CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG
    GGTTCCTGCC CAAACTCTCC TCGAGCTGAC AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50136
    Clone ID Related Accession (Same CDS sequence) XM_009292228.2
    Accession Version XM_009292228.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGGTGATGT GGGTATGTAA CCTGTGCAGG
    AAACAGCAGG AAATCCTAAC TAAATCGGGA GAATGGTTTT CATCAGGGCC CGGGGGGAGG
    CCTCTTAGTG TGGGTTCTCC CCTCAGTGGC CCTGAGTTGG AGAAGGAGAG GAAGTTGCGC
    TCCAGATCCC AGGTTCCGGG TGCCAACGAC ACTCTGCTAC CCAACGCTGG TCCCGGTGAC
    CATCCAAAGG GAGCAGATAC AATGCCCGCC TCACGCTCCC GCAGTGAACC ACCAAGAGAA
    AAGAGGAGGC CAGTGTCCAT GCATGAGCAG AATGGAAAAG TGATTGGACG TGGAGAGAGA
    AGAAGAGCTC CAGCCAGGTT ATCGTCGCAG GCCTCTGAAG ACCGAGCCCC AGGTGAGAAT
    CGAGATGGTA GACGACTAGA GAAAGACCAC TCACAGGAGG ACGATCCCAC CCACCCTGAG
    CGACGAAGAA GACAGGAGGA GGAAGAGCGA GAGCGGCAAA GACGAGAGGA AGAGTACCAG
    ACCCGTTACC GTAGTGACCC CAATCTAGCC CGCTACCCTG TTAAACCGCA GAAGGAGGAA
    CAAGAAATGC GCATGCATGC CAAAGTCTCC AAAATGCGCC AGGAGCGTCA TCACAGTGAC
    CTGGCCATCA ATGAAGTGGG CCTGATGCAG GATGGTGGCG AAGCTGCTGG AAATCGCATT
    AGCAGGCAAC GAGTAGCTAG CAATGAGGAC CGCAAGGCTC TTTTAGAAAA CCATCGAGCT
    TATTCTGTGG ACAGGACCGT GGGGGGTGGT GTTGGGGGGC AAGGGTCCCT CAAACAGGGC
    CATGGTGGCC CTCAAATGGG CCTTACTGGG CCTCCAGACC TCAGGGATGG ACAGGATTGG
    GGCCCAAAGG GGCATCTGGA ACCTGGGTCT GCAGCTTTTC TGCACAGGGC CAAGCGGGAA
    AAAGCTGCAG AAATCATGCG TAAAGATTCT TCTCTCAGCT CAGACCAGTC TGAGTCATTA
    CGGCCACCTC CACCAAGAGC CTACAGACCC AAACGAGGCC TCAACAAAAG GCAGATGTCC
    ATCAGTAGCT CAGAAGAAGA AGGAGGTTCC ACGCCTGAAT ACACCAGCTG TGAGGATGTG
    GAGATCGAAA GTGTTAGTGA AAAAGGTGAC TGGGACTGTC ATCCACTGGA CCCCACTGTG
    TGGCATCATC CAGTGAGTTG GCAGCCATCC AAAGAGGGAG ATCATTTGAT TGGCCGTATC
    ACTTTAAGTA AGCGATCCAC TATGCCACGA GAAGCCGGTT CTCTACTGGG TCTGAAGGTT
    GTTGGTGGGA AAATGACAGA GTCTGGTAGA CTGGGGGCCT TTATCACCAA AGTAAAGAAA
    GGAAGTTTGG CTGACATTGT CGGGCATTTA CGAGCAGGCG ATGAGGTGTT GCAGTGGAAT
    GGAAAAGCGT TGCCTGGAGC AACTAAAAAG GAAGTGTATA ATATTATTCT GGAGTCTCAG
    TCTGCACCTC AAGTGGAAAT AGTTGTTTCC AGACCGATTG GATGTATTCA ACGAATTCTA
    TCCAAAAACT TCTTGAAAAA GGTCTATAGA GCATATCAAG AAACGCCAAG ACTTCCAGGG
    ACATCACACC CCCCTCTAGA ATCAACTGGT TCGAGTTCTA TAGATGAATC TCAAAAGATG
    GATCGTCCAT CCATCTCTGT AATGTCTCCG ACTAGTCCCG GGATGTTGAG AGACCTCCCT
    CTTGTACTGC CAGGCCAGCT GTCGGTGAAA TTATGGTATG ATAAAGTGGG CCATCAGCTT
    ATTGTCAATG TTCTACAAGC ACGAGAACTG CCCCCTCGAC CTGATGGACG ACCCAGGAAT
    CCCTACGTCA AAATGTATTT TCTTCCTGAT AGAAGTGACA AAAGCAAAAG AAGGACAAAA
    ACGGTGAAGA AGAGTGCAGA GCCAAAGTGG AACCAGACGT TTCTGTATTC TCACGTCCAT
    CGCAGAGACT TCAGAGAACG CATGCTGGAG ATCACAGTTT GGGATCAGCC CAGAGCCCAG
    GAAGAAGAGA GTGATTTTCT GGGAGAGATT CTGATTGAGC TGGAAACAGC TCTGTTGGAT
    GATCAGGCAC ACTGGTACAA ATTTCAGACC CATGACGTGT CATCGCTTCC GTTGCCCCAA
    CCGTCCCCTT GCCTCCCGCG CAGACATGTT CATGGAGACA GTCCTAGTAA AAAACTGCAA
    AGATCTCATC GCATCATTGA AACAGATTAT GATGATGGTA TAACAGTAGT GTCCTCAGCA
    GCAGAAAGGA CCTCCAGAGA CAGAGAGAGG TCAAGCACAT TGACTGTACC TGAGAGACAG
    GCGGCCATAC AGCATCGCTC GCGGTCAGTT TCTCCGCACA GGGAAGACCA GTGTAGAGCC
    CGCTCTCGAC CCGCACACAT CCCTATGCAG AGGAGTCTGG ATGAGATCCA CCAGAACCGC
    CATCATTCCT ACTCTCCCTC CCATTACCAC GACTCCCACC AGGAACATCG ATCCGGAGAC
    TCGGACTATG AATATTCAGA GGACAGCGAG GTCCTGGAGA TGCACAGATC AATCCGAGGT
    GGGAGTGCTG AGTGCCTGCA TACAAACAGG AAATTAGCTA GACACTGTAA CACACTTCCC
    CCAAAGATGC CATTGTTAGT GAACGGTATA CATAAAGATA TTTACAGCTC TACCCTTCCT
    GCATGCTTAA AGAGCAAAGC ACCACGACGA ACAGAGAGGG ATGAAGTTGT CCCTCTTAGT
    GCCATTCCAC AGCGCATTCT CAGGTTCACA GACGAGGTCA GCTCTGGTGA TCTTCAGCCT
    TCATTAGACA GAGTAAGAAG TGCCAGCACA ACTTGTTTGA GACCAGAGTC AAGTTTTCAT
    TCACCTGAAA GTCATTTTCC ATTTCACAGG CATTCTGGCA ATTTGGATAG GCCAAGCTGT
    CAGATAGCTA ACAAGAAAAC CATTGATGGT GACAGACTTC AGCCCACCTC TATCCTGAGA
    GGCAGCAGGA GGAGGAGAGA GACCCCCCTC AGGCCCTTTG GCCAGACCAG CTTAGGGGGT
    TCCTGCCCAA ACTCTCCTCG AGCTGACAGA GATCTTCCAC ACGGAGGACA TTATTCTCCA
    GCAGGGACCA CATCATTAGG GCGCAGGGGC CGACAGCTGC CACAACTCCC TGCAAAGAGC
    AGCAGTATAG AACAAGGCCG TATACCTGAG GAGAAACCTA ACTCAGCTCT AGCAGTGGAG
    GAGCGGGCTC GTCAGTTGCA GATGAGAGTG CACTCGTATC GGCCGGGATC TTCTGCCAAT
    TCTGCTCATG ATCCAGAGGC AGAGCTTAAG AACAGGAGAG ATATGTACAA GGACCAGCGG
    AGGAGCTGTG ACAACATGTC TGCGAGGTCA TCAGACAGTG ACATGAGCGA CGTATCGGCC
    ATCTCTCATG CCAGCAGTGC CTCACGGCTG AGTGCCACCA GCTACATGTC CATTCAGTCT
    GAAAGGCCAC ACGGGAGAAT CAGGTCAAAG TCATTGGAAA GTTGTAAAGG AGAAGAAAAG
    AAAGAAAGGA GGATTTCGTG GGGGGTGAAT GGGACAGACA ACAGAAGGAG TTTGGGAAAG
    AGACGTTTCT CTGAGGAGCT TAAACGCAGA CGGCACACGG TGGCTGGAGA TGTGAGAGCC
    ATCTTTGCAT ACCTCACCTC ATACTTTCCA TGCTTGAATG CTGAGCCGGC ACTGGATCCC
    CCCAAACCCA GAGCCCTATC TGCGCTTCAG CCTGTGGAAG AGAGACCCAC CTCCCAGCTC
    CCAATCCCCA CCCTTCGTCC CACAGATAGA GAGTTAGGGT GTAGAGGTGA TGGAGAAAGT
    GCAGTGGGAC AGTCCTGCAG TACACTGACT CCATCAGAGA CAGATACACG ACAGCTCCTT
    CAAACCTCCA GGGGACAGCA GAGGACATGG CTGCGCTGCA ATAGTGCACT GCTGTGTCAT
    AATACAGACT GTTTGAACAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290503.1
    CDS1261..5604
    Translation

    Target ORF information:

    RefSeq Version XM_009292228.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292228.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGGTGATGT GGGTATGTAA CCTGTGCAGG
    AAACAGCAGG AAATCCTAAC TAAATCGGGA GAATGGTTTT CATCAGGGCC CGGGGGGAGG
    CCTCTTAGTG TGGGTTCTCC CCTCAGTGGC CCTGAGTTGG AGAAGGAGAG GAAGTTGCGC
    TCCAGATCCC AGGTTCCGGG TGCCAACGAC ACTCTGCTAC CCAACGCTGG TCCCGGTGAC
    CATCCAAAGG GAGCAGATAC AATGCCCGCC TCACGCTCCC GCAGTGAACC ACCAAGAGAA
    AAGAGGAGGC CAGTGTCCAT GCATGAGCAG AATGGAAAAG TGATTGGACG TGGAGAGAGA
    AGAAGAGCTC CAGCCAGGTT ATCGTCGCAG GCCTCTGAAG ACCGAGCCCC AGGTGAGAAT
    CGAGATGGTA GACGACTAGA GAAAGACCAC TCACAGGAGG ACGATCCCAC CCACCCTGAG
    CGACGAAGAA GACAGGAGGA GGAAGAGCGA GAGCGGCAAA GACGAGAGGA AGAGTACCAG
    ACCCGTTACC GTAGTGACCC CAATCTAGCC CGCTACCCTG TTAAACCGCA GAAGGAGGAA
    CAAGAAATGC GCATGCATGC CAAAGTCTCC AAAATGCGCC AGGAGCGTCA TCACAGTGAC
    CTGGCCATCA ATGAAGTGGG CCTGATGCAG GATGGTGGCG AAGCTGCTGG AAATCGCATT
    AGCAGGCAAC GAGTAGCTAG CAATGAGGAC CGCAAGGCTC TTTTAGAAAA CCATCGAGCT
    TATTCTGTGG ACAGGACCGT GGGGGGTGGT GTTGGGGGGC AAGGGTCCCT CAAACAGGGC
    CATGGTGGCC CTCAAATGGG CCTTACTGGG CCTCCAGACC TCAGGGATGG ACAGGATTGG
    GGCCCAAAGG GGCATCTGGA ACCTGGGTCT GCAGCTTTTC TGCACAGGGC CAAGCGGGAA
    AAAGCTGCAG AAATCATGCG TAAAGATTCT TCTCTCAGCT CAGACCAGTC TGAGTCATTA
    CGGCCACCTC CACCAAGAGC CTACAGACCC AAACGAGGCC TCAACAAAAG GCAGATGTCC
    ATCAGTAGCT CAGAAGAAGA AGGAGGTTCC ACGCCTGAAT ACACCAGCTG TGAGGATGTG
    GAGATCGAAA GTGTTAGTGA AAAAGGTGAC TGGGACTGTC ATCCACTGGA CCCCACTGTG
    TGGCATCATC CAGTGAGTTG GCAGCCATCC AAAGAGGGAG ATCATTTGAT TGGCCGTATC
    ACTTTAAGTA AGCGATCCAC TATGCCACGA GAAGCCGGTT CTCTACTGGG TCTGAAGGTT
    GTTGGTGGGA AAATGACAGA GTCTGGTAGA CTGGGGGCCT TTATCACCAA AGTAAAGAAA
    GGAAGTTTGG CTGACATTGT CGGGCATTTA CGAGCAGGCG ATGAGGTGTT GCAGTGGAAT
    GGAAAAGCGT TGCCTGGAGC AACTAAAAAG GAAGTGTATA ATATTATTCT GGAGTCTCAG
    TCTGCACCTC AAGTGGAAAT AGTTGTTTCC AGACCGATTG GATGTATTCA ACGAATTCTA
    TCCAAAAACT TCTTGAAAAA GGTCTATAGA GCATATCAAG AAACGCCAAG ACTTCCAGGG
    ACATCACACC CCCCTCTAGA ATCAACTGGT TCGAGTTCTA TAGATGAATC TCAAAAGATG
    GATCGTCCAT CCATCTCTGT AATGTCTCCG ACTAGTCCCG GGATGTTGAG AGACCTCCCT
    CTTGTACTGC CAGGCCAGCT GTCGGTGAAA TTATGGTATG ATAAAGTGGG CCATCAGCTT
    ATTGTCAATG TTCTACAAGC ACGAGAACTG CCCCCTCGAC CTGATGGACG ACCCAGGAAT
    CCCTACGTCA AAATGTATTT TCTTCCTGAT AGAAGTGACA AAAGCAAAAG AAGGACAAAA
    ACGGTGAAGA AGAGTGCAGA GCCAAAGTGG AACCAGACGT TTCTGTATTC TCACGTCCAT
    CGCAGAGACT TCAGAGAACG CATGCTGGAG ATCACAGTTT GGGATCAGCC CAGAGCCCAG
    GAAGAAGAGA GTGATTTTCT GGGAGAGATT CTGATTGAGC TGGAAACAGC TCTGTTGGAT
    GATCAGGCAC ACTGGTACAA ATTTCAGACC CATGACGTGT CATCGCTTCC GTTGCCCCAA
    CCGTCCCCTT GCCTCCCGCG CAGACATGTT CATGGAGACA GTCCTAGTAA AAAACTGCAA
    AGATCTCATC GCATCATTGA AACAGATTAT GATGATGGTA TAACAGTAGT GTCCTCAGCA
    GCAGAAAGGA CCTCCAGAGA CAGAGAGAGG TCAAGCACAT TGACTGTACC TGAGAGACAG
    GCGGCCATAC AGCATCGCTC GCGGTCAGTT TCTCCGCACA GGGAAGACCA GTGTAGAGCC
    CGCTCTCGAC CCGCACACAT CCCTATGCAG AGGAGTCTGG ATGAGATCCA CCAGAACCGC
    CATCATTCCT ACTCTCCCTC CCATTACCAC GACTCCCACC AGGAACATCG ATCCGGAGAC
    TCGGACTATG AATATTCAGA GGACAGCGAG GTCCTGGAGA TGCACAGATC AATCCGAGGT
    GGGAGTGCTG AGTGCCTGCA TACAAACAGG AAATTAGCTA GACACTGTAA CACACTTCCC
    CCAAAGATGC CATTGTTAGT GAACGGTATA CATAAAGATA TTTACAGCTC TACCCTTCCT
    GCATGCTTAA AGAGCAAAGC ACCACGACGA ACAGAGAGGG ATGAAGTTGT CCCTCTTAGT
    GCCATTCCAC AGCGCATTCT CAGGTTCACA GACGAGGTCA GCTCTGGTGA TCTTCAGCCT
    TCATTAGACA GAGTAAGAAG TGCCAGCACA ACTTGTTTGA GACCAGAGTC AAGTTTTCAT
    TCACCTGAAA GTCATTTTCC ATTTCACAGG CATTCTGGCA ATTTGGATAG GCCAAGCTGT
    CAGATAGCTA ACAAGAAAAC CATTGATGGT GACAGACTTC AGCCCACCTC TATCCTGAGA
    GGCAGCAGGA GGAGGAGAGA GACCCCCCTC AGGCCCTTTG GCCAGACCAG CTTAGGGGGT
    TCCTGCCCAA ACTCTCCTCG AGCTGACAGA GATCTTCCAC ACGGAGGACA TTATTCTCCA
    GCAGGGACCA CATCATTAGG GCGCAGGGGC CGACAGCTGC CACAACTCCC TGCAAAGAGC
    AGCAGTATAG AACAAGGCCG TATACCTGAG GAGAAACCTA ACTCAGCTCT AGCAGTGGAG
    GAGCGGGCTC GTCAGTTGCA GATGAGAGTG CACTCGTATC GGCCGGGATC TTCTGCCAAT
    TCTGCTCATG ATCCAGAGGC AGAGCTTAAG AACAGGAGAG ATATGTACAA GGACCAGCGG
    AGGAGCTGTG ACAACATGTC TGCGAGGTCA TCAGACAGTG ACATGAGCGA CGTATCGGCC
    ATCTCTCATG CCAGCAGTGC CTCACGGCTG AGTGCCACCA GCTACATGTC CATTCAGTCT
    GAAAGGCCAC ACGGGAGAAT CAGGTCAAAG TCATTGGAAA GTTGTAAAGG AGAAGAAAAG
    AAAGAAAGGA GGATTTCGTG GGGGGTGAAT GGGACAGACA ACAGAAGGAG TTTGGGAAAG
    AGACGTTTCT CTGAGGAGCT TAAACGCAGA CGGCACACGG TGGCTGGAGA TGTGAGAGCC
    ATCTTTGCAT ACCTCACCTC ATACTTTCCA TGCTTGAATG CTGAGCCGGC ACTGGATCCC
    CCCAAACCCA GAGCCCTATC TGCGCTTCAG CCTGTGGAAG AGAGACCCAC CTCCCAGCTC
    CCAATCCCCA CCCTTCGTCC CACAGATAGA GAGTTAGGGT GTAGAGGTGA TGGAGAAAGT
    GCAGTGGGAC AGTCCTGCAG TACACTGACT CCATCAGAGA CAGATACACG ACAGCTCCTT
    CAAACCTCCA GGGGACAGCA GAGGACATGG CTGCGCTGCA ATAGTGCACT GCTGTGTCAT
    AATACAGACT GTTTGAACAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50137
    Clone ID Related Accession (Same CDS sequence) XM_009292229.2
    Accession Version XM_009292229.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGCTC TAGCAGTGGA GGAGCGGGCT
    CGTCAGTTGC AGATGAGAGT GCACTCGTAT CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT
    GATCCAGAGG CAGAGCTTAA GAACAGGAGA GATATGTACA AGGACCAGCG GAGGAGCTGT
    GACAACATGT CTGCGAGGTC ATCAGACAGT GACATGAGCG ACGTATCGGC CATCTCTCAT
    GCCAGCAGTG CCTCACGGCT GAGTGCCACC AGCTACATGT CCATTCAGTC TGAAAGGCCA
    CACGGGAGAA TCAGGTCAAA GTCATTGGAA AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG
    AGGATTTCGT GGGGGGTGAA TGGGACAGAC AACAGAAGGA GTTTGGGAAA GAGACGTTTC
    TCTGAGGAGC TTAAACGCAG ACGGCACACG GTGGCTGGAG ATGTGAGAGC CATCTTTGCA
    TACCTCACCT CATACTTTCC ATGCTTGAAT GCTGAGCCGG CACTGGATCC CCCCAAACCC
    AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA GAGAGACCCA CCTCCCAGCT CCCAATCCCC
    ACCCTTCGTC CCACAGATAG AGAGTTAGGG TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA
    CAGTCCTGCA GTACACTGAC TCCATCAGAG ACAGATACAC GACAGCTCCT TCAAACCTCC
    AGGGGACAGC AGAGGACATG GCTGCGCTGC AATAGTGCAC TGCTGTGTCA TAATACAGAC
    TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290504.1
    CDS1261..5595
    Translation

    Target ORF information:

    RefSeq Version XM_009292229.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGCTC TAGCAGTGGA GGAGCGGGCT
    CGTCAGTTGC AGATGAGAGT GCACTCGTAT CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT
    GATCCAGAGG CAGAGCTTAA GAACAGGAGA GATATGTACA AGGACCAGCG GAGGAGCTGT
    GACAACATGT CTGCGAGGTC ATCAGACAGT GACATGAGCG ACGTATCGGC CATCTCTCAT
    GCCAGCAGTG CCTCACGGCT GAGTGCCACC AGCTACATGT CCATTCAGTC TGAAAGGCCA
    CACGGGAGAA TCAGGTCAAA GTCATTGGAA AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG
    AGGATTTCGT GGGGGGTGAA TGGGACAGAC AACAGAAGGA GTTTGGGAAA GAGACGTTTC
    TCTGAGGAGC TTAAACGCAG ACGGCACACG GTGGCTGGAG ATGTGAGAGC CATCTTTGCA
    TACCTCACCT CATACTTTCC ATGCTTGAAT GCTGAGCCGG CACTGGATCC CCCCAAACCC
    AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA GAGAGACCCA CCTCCCAGCT CCCAATCCCC
    ACCCTTCGTC CCACAGATAG AGAGTTAGGG TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA
    CAGTCCTGCA GTACACTGAC TCCATCAGAG ACAGATACAC GACAGCTCCT TCAAACCTCC
    AGGGGACAGC AGAGGACATG GCTGCGCTGC AATAGTGCAC TGCTGTGTCA TAATACAGAC
    TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50138
    Clone ID Related Accession (Same CDS sequence) XM_009292230.2
    Accession Version XM_009292230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGTGATGTGG GTATGTAACC TGTGCAGGAA ACAGCAGGAA
    ATCCTAACTA AATCGGGAGA ATGGTTTTCA TCAGGGCCCG GGGGGAGGCC TCTTAGTGTG
    GGTTCTCCCC TCAGTGGCCC TGAGTTGGAG AAGGAGAGGA AGTTGCGCTC CAGATCCCAG
    GTTCCGGGTG CCAACGACAC TCTGCTACCC AACGCTGGTC CCGGTGACCA TCCAAAGGGA
    GCAGATACAA TGCCCGCCTC ACGCTCCCGC AGTGAACCAC CAAGAGAAAA GAGGAGGCCA
    GTGTCCATGC ATGAGCAGAA TGGAAAAGTG ATTGGACGTG GAGAGAGAAG AAGAGCTCCA
    GCCAGGTTAT CGTCGCAGGC CTCTGAAGAC CGAGCCCCAG GTGAGAATCG AGATGGTAGA
    CGACTAGAGA AAGACCACTC ACAGGAGGAC GATCCCACCC ACCCTGAGCG ACGAAGAAGA
    CAGGAGGAGG AAGAGCGAGA GCGGCAAAGA CGAGAGGAAG AGTACCAGAC CCGTTACCGT
    AGTGACCCCA ATCTAGCCCG CTACCCTGTT AAACCGCAGA AGGAGGAACA AGAAATGCGC
    ATGCATGCCA AAGTCTCCAA AATGCGCCAG GAGCGTCATC ACAGTGACCT GGCCATCAAT
    GAAGTGGGCC TGATGCAGGA TGGTGGCGAA GCTGCTGGAA ATCGCATTAG CAGGCAACGA
    GTAGCTAGCA ATGAGGACCG CAAGGCTCTT TTAGAAAACC ATCGAGCTTA TTCTGTGGAC
    AGGACCGTGG GGGGTGGTGT TGGGGGGCAA GGGTCCCTCA AACAGGGCCA TGGTGGCCCT
    CAAATGGGCC TTACTGGGCC TCCAGACCTC AGGGATGGAC AGGATTGGGG CCCAAAGGGG
    CATCTGGAAC CTGGGTCTGC AGCTTTTCTG CACAGGGCCA AGCGGGAAAA AGCTGCAGAA
    ATCATGCGTA AAGATTCTTC TCTCAGCTCA GACCAGTCTG AGTCATTACG GCCACCTCCA
    CCAAGAGCCT ACAGACCCAA ACGAGGCCTC AACAAAAGGC AGATGTCCAT CAGTAGCTCA
    GAAGAAGAAG GAGGTTCCAC GCCTGAATAC ACCAGCTGTG AGGATGTGGA GATCGAAAGT
    GTTAGTGAAA AAGGTGACTG GGACTGTCAT CCACTGGACC CCACTGTGTG GCATCATCCA
    GTGAGTTGGC AGCCATCCAA AGAGGGAGAT CATTTGATTG GCCGTATCAC TTTAAGTAAG
    CGATCCACTA TGCCACGAGA AGCCGGTTCT CTACTGGGTC TGAAGGTTGT TGGTGGGAAA
    ATGACAGAGT CTGGTAGACT GGGGGCCTTT ATCACCAAAG TAAAGAAAGG AAGTTTGGCT
    GACATTGTCG GGCATTTACG AGCAGGCGAT GAGGTGTTGC AGTGGAATGG AAAAGCGTTG
    CCTGGAGCAA CTAAAAAGGA AGTGTATAAT ATTATTCTGG AGTCTCAGTC TGCACCTCAA
    GTGGAAATAG TTGTTTCCAG ACCGATTGGA TGTATTCAAC GAATTCTATC CAAAAACTTC
    TTGAAAAAGG TCTATAGAGC ATATCAAGAA ACGCCAAGAC TTCCAGGGAC ATCACACCCC
    CCTCTAGAAT CAACTGGTTC GAGTTCTATA GATGAATCTC AAAAGATGGA TCGTCCATCC
    ATCTCTGTAA TGTCTCCGAC TAGTCCCGGG ATGTTGAGAG ACCTCCCTCT TGTACTGCCA
    GGCCAGCTGT CGGTGAAATT ATGGTATGAT AAAGTGGGCC ATCAGCTTAT TGTCAATGTT
    CTACAAGCAC GAGAACTGCC CCCTCGACCT GATGGACGAC CCAGGAATCC CTACGTCAAA
    ATGTATTTTC TTCCTGATAG AAGTGACAAA AGCAAAAGAA GGACAAAAAC GGTGAAGAAG
    AGTGCAGAGC CAAAGTGGAA CCAGACGTTT CTGTATTCTC ACGTCCATCG CAGAGACTTC
    AGAGAACGCA TGCTGGAGAT CACAGTTTGG GATCAGCCCA GAGCCCAGGA AGAAGAGAGT
    GATTTTCTGG GAGAGATTCT GATTGAGCTG GAAACAGCTC TGTTGGATGA TCAGGCACAC
    TGGTACAAAT TTCAGACCCA TGACGTGTCA TCGCTTCCGT TGCCCCAACC GTCCCCTTGC
    CTCCCGCGCA GACATGTTCA TGGAGACAGT CCTAGTAAAA AACTGCAAAG ATCTCATCGC
    ATCATTGAAA CAGATTATGA TGATGGTATA ACAGTAGTGT CCTCAGCAGC AGAAAGGACC
    TCCAGAGACA GAGAGAGGTC AAGCACATTG ACTGTACCTG AGAGACAGGC GGCCATACAG
    CATCGCTCGC GGTCAGTTTC TCCGCACAGG GAAGACCAGT GTAGAGCCCG CTCTCGACCC
    GCACACATCC CTATGCAGAG GAGTCTGGAT GAGATCCACC AGAACCGCCA TCATTCCTAC
    TCTCCCTCCC ATTACCACGA CTCCCACCAG GAACATCGAT CCGGAGACTC GGACTATGAA
    TATTCAGAGG ACAGCGAGGT CCTGGAGATG CACAGATCAA TCCGAGGTGG GAGTGCTGAG
    TGCCTGCATA CAAACAGGAA ATTAGCTAGA CACTGTAACA CACTTCCCCC AAAGATGCCA
    TTGTTAGTGA ACGGTATACA TAAAGATATT TACAGCTCTA CCCTTCCTGC ATGCTTAAAG
    AGCAAAGCAC CACGACGAAC AGAGAGGGAT GAAGTTGTCC CTCTTAGTGC CATTCCACAG
    CGCATTCTCA GGTTCACAGA CGAGGTCAGC TCTGGTGATC TTCAGCCTTC ATTAGACAGA
    GTAAGAAGTG CCAGCACAAC TTGTTTGAGA CCAGAGTCAA GTTTTCATTC ACCTGAAAGT
    CATTTTCCAT TTCACAGGCA TTCTGGCAAT TTGGATAGGC CAAGCTGTCA GATAGCTAAC
    AAGAAAACCA TTGATGGTGA CAGACTTCAG CCCACCTCTA TCCTGAGAGG CAGCAGGAGG
    AGGAGAGAGA CCCCCCTCAG GCCCTTTGGC CAGACCAGCT TAGGGGGTTC CTGCCCAAAC
    TCTCCTCGAG CTGACAGAGA TCTTCCACAC GGAGGACATT ATTCTCCAGC AGGGACCACA
    TCATTAGGGC GCAGGGGCCG ACAGCTGCCA CAACTCCCTG CAAAGAGCAG CAGTATAGAA
    CAAGGCCGTA TACCTGAGGA GAAACCTAAC TCAGCTCTAG CAGTGGAGGA GCGGGCTCGT
    CAGTTGCAGA TGAGAGTGCA CTCGTATCGG CCGGGATCTT CTGCCAATTC TGCTCATGAT
    CCAGAGGCAG AGCTTAAGAA CAGGAGAGAT ATGTACAAGG ACCAGCGGAG GAGCTGTGAC
    AACATGTCTG CGAGGTCATC AGACAGTGAC ATGAGCGACG TATCGGCCAT CTCTCATGCC
    AGCAGTGCCT CACGGCTGAG TGCCACCAGC TACATGTCCA TTCAGTCTGA AAGGCCACAC
    GGGAGAATCA GGTCAAAGTC ATTGGAAAGT TGTAAAGGAG AAGAAAAGAA AGAAAGGAGG
    ATTTCGTGGG GGGTGAATGG GACAGACAAC AGAAGGAGTT TGGGAAAGAG ACGTTTCTCT
    GAGGAGCTTA AACGCAGACG GCACACGGTG GCTGGAGATG TGAGAGCCAT CTTTGCATAC
    CTCACCTCAT ACTTTCCATG CTTGAATGCT GAGCCGGCAC TGGATCCCCC CAAACCCAGA
    GCCCTATCTG CGCTTCAGCC TGTGGAAGAG AGACCCACCT CCCAGCTCCC AATCCCCACC
    CTTCGTCCCA CAGATAGAGA GTTAGGGTGT AGAGGTGATG GAGAAAGTGC AGTGGGACAG
    TCCTGCAGTA CACTGACTCC ATCAGAGACA GATACACGAC AGCTCCTTCA AACCTCCAGG
    GGACAGCAGA GGACATGGCT GCGCTGCAAT AGTGCACTGC TGTGTCATAA TACAGACTGT
    TTGAACAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290505.1
    CDS1261..5592
    Translation

    Target ORF information:

    RefSeq Version XM_009292230.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGTGATGTGG GTATGTAACC TGTGCAGGAA ACAGCAGGAA
    ATCCTAACTA AATCGGGAGA ATGGTTTTCA TCAGGGCCCG GGGGGAGGCC TCTTAGTGTG
    GGTTCTCCCC TCAGTGGCCC TGAGTTGGAG AAGGAGAGGA AGTTGCGCTC CAGATCCCAG
    GTTCCGGGTG CCAACGACAC TCTGCTACCC AACGCTGGTC CCGGTGACCA TCCAAAGGGA
    GCAGATACAA TGCCCGCCTC ACGCTCCCGC AGTGAACCAC CAAGAGAAAA GAGGAGGCCA
    GTGTCCATGC ATGAGCAGAA TGGAAAAGTG ATTGGACGTG GAGAGAGAAG AAGAGCTCCA
    GCCAGGTTAT CGTCGCAGGC CTCTGAAGAC CGAGCCCCAG GTGAGAATCG AGATGGTAGA
    CGACTAGAGA AAGACCACTC ACAGGAGGAC GATCCCACCC ACCCTGAGCG ACGAAGAAGA
    CAGGAGGAGG AAGAGCGAGA GCGGCAAAGA CGAGAGGAAG AGTACCAGAC CCGTTACCGT
    AGTGACCCCA ATCTAGCCCG CTACCCTGTT AAACCGCAGA AGGAGGAACA AGAAATGCGC
    ATGCATGCCA AAGTCTCCAA AATGCGCCAG GAGCGTCATC ACAGTGACCT GGCCATCAAT
    GAAGTGGGCC TGATGCAGGA TGGTGGCGAA GCTGCTGGAA ATCGCATTAG CAGGCAACGA
    GTAGCTAGCA ATGAGGACCG CAAGGCTCTT TTAGAAAACC ATCGAGCTTA TTCTGTGGAC
    AGGACCGTGG GGGGTGGTGT TGGGGGGCAA GGGTCCCTCA AACAGGGCCA TGGTGGCCCT
    CAAATGGGCC TTACTGGGCC TCCAGACCTC AGGGATGGAC AGGATTGGGG CCCAAAGGGG
    CATCTGGAAC CTGGGTCTGC AGCTTTTCTG CACAGGGCCA AGCGGGAAAA AGCTGCAGAA
    ATCATGCGTA AAGATTCTTC TCTCAGCTCA GACCAGTCTG AGTCATTACG GCCACCTCCA
    CCAAGAGCCT ACAGACCCAA ACGAGGCCTC AACAAAAGGC AGATGTCCAT CAGTAGCTCA
    GAAGAAGAAG GAGGTTCCAC GCCTGAATAC ACCAGCTGTG AGGATGTGGA GATCGAAAGT
    GTTAGTGAAA AAGGTGACTG GGACTGTCAT CCACTGGACC CCACTGTGTG GCATCATCCA
    GTGAGTTGGC AGCCATCCAA AGAGGGAGAT CATTTGATTG GCCGTATCAC TTTAAGTAAG
    CGATCCACTA TGCCACGAGA AGCCGGTTCT CTACTGGGTC TGAAGGTTGT TGGTGGGAAA
    ATGACAGAGT CTGGTAGACT GGGGGCCTTT ATCACCAAAG TAAAGAAAGG AAGTTTGGCT
    GACATTGTCG GGCATTTACG AGCAGGCGAT GAGGTGTTGC AGTGGAATGG AAAAGCGTTG
    CCTGGAGCAA CTAAAAAGGA AGTGTATAAT ATTATTCTGG AGTCTCAGTC TGCACCTCAA
    GTGGAAATAG TTGTTTCCAG ACCGATTGGA TGTATTCAAC GAATTCTATC CAAAAACTTC
    TTGAAAAAGG TCTATAGAGC ATATCAAGAA ACGCCAAGAC TTCCAGGGAC ATCACACCCC
    CCTCTAGAAT CAACTGGTTC GAGTTCTATA GATGAATCTC AAAAGATGGA TCGTCCATCC
    ATCTCTGTAA TGTCTCCGAC TAGTCCCGGG ATGTTGAGAG ACCTCCCTCT TGTACTGCCA
    GGCCAGCTGT CGGTGAAATT ATGGTATGAT AAAGTGGGCC ATCAGCTTAT TGTCAATGTT
    CTACAAGCAC GAGAACTGCC CCCTCGACCT GATGGACGAC CCAGGAATCC CTACGTCAAA
    ATGTATTTTC TTCCTGATAG AAGTGACAAA AGCAAAAGAA GGACAAAAAC GGTGAAGAAG
    AGTGCAGAGC CAAAGTGGAA CCAGACGTTT CTGTATTCTC ACGTCCATCG CAGAGACTTC
    AGAGAACGCA TGCTGGAGAT CACAGTTTGG GATCAGCCCA GAGCCCAGGA AGAAGAGAGT
    GATTTTCTGG GAGAGATTCT GATTGAGCTG GAAACAGCTC TGTTGGATGA TCAGGCACAC
    TGGTACAAAT TTCAGACCCA TGACGTGTCA TCGCTTCCGT TGCCCCAACC GTCCCCTTGC
    CTCCCGCGCA GACATGTTCA TGGAGACAGT CCTAGTAAAA AACTGCAAAG ATCTCATCGC
    ATCATTGAAA CAGATTATGA TGATGGTATA ACAGTAGTGT CCTCAGCAGC AGAAAGGACC
    TCCAGAGACA GAGAGAGGTC AAGCACATTG ACTGTACCTG AGAGACAGGC GGCCATACAG
    CATCGCTCGC GGTCAGTTTC TCCGCACAGG GAAGACCAGT GTAGAGCCCG CTCTCGACCC
    GCACACATCC CTATGCAGAG GAGTCTGGAT GAGATCCACC AGAACCGCCA TCATTCCTAC
    TCTCCCTCCC ATTACCACGA CTCCCACCAG GAACATCGAT CCGGAGACTC GGACTATGAA
    TATTCAGAGG ACAGCGAGGT CCTGGAGATG CACAGATCAA TCCGAGGTGG GAGTGCTGAG
    TGCCTGCATA CAAACAGGAA ATTAGCTAGA CACTGTAACA CACTTCCCCC AAAGATGCCA
    TTGTTAGTGA ACGGTATACA TAAAGATATT TACAGCTCTA CCCTTCCTGC ATGCTTAAAG
    AGCAAAGCAC CACGACGAAC AGAGAGGGAT GAAGTTGTCC CTCTTAGTGC CATTCCACAG
    CGCATTCTCA GGTTCACAGA CGAGGTCAGC TCTGGTGATC TTCAGCCTTC ATTAGACAGA
    GTAAGAAGTG CCAGCACAAC TTGTTTGAGA CCAGAGTCAA GTTTTCATTC ACCTGAAAGT
    CATTTTCCAT TTCACAGGCA TTCTGGCAAT TTGGATAGGC CAAGCTGTCA GATAGCTAAC
    AAGAAAACCA TTGATGGTGA CAGACTTCAG CCCACCTCTA TCCTGAGAGG CAGCAGGAGG
    AGGAGAGAGA CCCCCCTCAG GCCCTTTGGC CAGACCAGCT TAGGGGGTTC CTGCCCAAAC
    TCTCCTCGAG CTGACAGAGA TCTTCCACAC GGAGGACATT ATTCTCCAGC AGGGACCACA
    TCATTAGGGC GCAGGGGCCG ACAGCTGCCA CAACTCCCTG CAAAGAGCAG CAGTATAGAA
    CAAGGCCGTA TACCTGAGGA GAAACCTAAC TCAGCTCTAG CAGTGGAGGA GCGGGCTCGT
    CAGTTGCAGA TGAGAGTGCA CTCGTATCGG CCGGGATCTT CTGCCAATTC TGCTCATGAT
    CCAGAGGCAG AGCTTAAGAA CAGGAGAGAT ATGTACAAGG ACCAGCGGAG GAGCTGTGAC
    AACATGTCTG CGAGGTCATC AGACAGTGAC ATGAGCGACG TATCGGCCAT CTCTCATGCC
    AGCAGTGCCT CACGGCTGAG TGCCACCAGC TACATGTCCA TTCAGTCTGA AAGGCCACAC
    GGGAGAATCA GGTCAAAGTC ATTGGAAAGT TGTAAAGGAG AAGAAAAGAA AGAAAGGAGG
    ATTTCGTGGG GGGTGAATGG GACAGACAAC AGAAGGAGTT TGGGAAAGAG ACGTTTCTCT
    GAGGAGCTTA AACGCAGACG GCACACGGTG GCTGGAGATG TGAGAGCCAT CTTTGCATAC
    CTCACCTCAT ACTTTCCATG CTTGAATGCT GAGCCGGCAC TGGATCCCCC CAAACCCAGA
    GCCCTATCTG CGCTTCAGCC TGTGGAAGAG AGACCCACCT CCCAGCTCCC AATCCCCACC
    CTTCGTCCCA CAGATAGAGA GTTAGGGTGT AGAGGTGATG GAGAAAGTGC AGTGGGACAG
    TCCTGCAGTA CACTGACTCC ATCAGAGACA GATACACGAC AGCTCCTTCA AACCTCCAGG
    GGACAGCAGA GGACATGGCT GCGCTGCAAT AGTGCACTGC TGTGTCATAA TACAGACTGT
    TTGAACAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50139
    Clone ID Related Accession (Same CDS sequence) XM_009292231.2
    Accession Version XM_009292231.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGAGAAACGC CAAGACTTCC AGGGACATCA CACCCCCCTC TAGAATCAAC TGGTTCGAGT
    TCTATAGATG AATCTCAAAA GATGGATCGT CCATCCATCT CTGTAATGTC TCCGACTAGT
    CCCGGGATGT TGAGAGACCT CCCTCTTGTA CTGCCAGGCC AGCTGTCGGT GAAATTATGG
    TATGATAAAG TGGGCCATCA GCTTATTGTC AATGTTCTAC AAGCACGAGA ACTGCCCCCT
    CGACCTGATG GACGACCCAG GAATCCCTAC GTCAAAATGT ATTTTCTTCC TGATAGAAGT
    GACAAAAGCA AAAGAAGGAC AAAAACGGTG AAGAAGAGTG CAGAGCCAAA GTGGAACCAG
    ACGTTTCTGT ATTCTCACGT CCATCGCAGA GACTTCAGAG AACGCATGCT GGAGATCACA
    GTTTGGGATC AGCCCAGAGC CCAGGAAGAA GAGAGTGATT TTCTGGGAGA GATTCTGATT
    GAGCTGGAAA CAGCTCTGTT GGATGATCAG GCACACTGGT ACAAATTTCA GACCCATGAC
    GTGTCATCGC TTCCGTTGCC CCAACCGTCC CCTTGCCTCC CGCGCAGACA TGTTCATGGA
    GACAGTCCTA GTAAAAAACT GCAAAGATCT CATCGCATCA TTGAAACAGA TTATGATGAT
    GGTATAACAG TAGTGTCCTC AGCAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290506.1
    CDS1261..5568
    Translation

    Target ORF information:

    RefSeq Version XM_009292231.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGAGAAACGC CAAGACTTCC AGGGACATCA CACCCCCCTC TAGAATCAAC TGGTTCGAGT
    TCTATAGATG AATCTCAAAA GATGGATCGT CCATCCATCT CTGTAATGTC TCCGACTAGT
    CCCGGGATGT TGAGAGACCT CCCTCTTGTA CTGCCAGGCC AGCTGTCGGT GAAATTATGG
    TATGATAAAG TGGGCCATCA GCTTATTGTC AATGTTCTAC AAGCACGAGA ACTGCCCCCT
    CGACCTGATG GACGACCCAG GAATCCCTAC GTCAAAATGT ATTTTCTTCC TGATAGAAGT
    GACAAAAGCA AAAGAAGGAC AAAAACGGTG AAGAAGAGTG CAGAGCCAAA GTGGAACCAG
    ACGTTTCTGT ATTCTCACGT CCATCGCAGA GACTTCAGAG AACGCATGCT GGAGATCACA
    GTTTGGGATC AGCCCAGAGC CCAGGAAGAA GAGAGTGATT TTCTGGGAGA GATTCTGATT
    GAGCTGGAAA CAGCTCTGTT GGATGATCAG GCACACTGGT ACAAATTTCA GACCCATGAC
    GTGTCATCGC TTCCGTTGCC CCAACCGTCC CCTTGCCTCC CGCGCAGACA TGTTCATGGA
    GACAGTCCTA GTAAAAAACT GCAAAGATCT CATCGCATCA TTGAAACAGA TTATGATGAT
    GGTATAACAG TAGTGTCCTC AGCAGCAGAA AGGACCTCCA GAGACAGAGA GAGGTCAAGC
    ACATTGACTG TACCTGAGAG ACAGGCGGCC ATACAGCATC GCTCGCGGTC AGTTTCTCCG
    CACAGGGAAG ACCAGTGTAG AGCCCGCTCT CGACCCGCAC ACATCCCTAT GCAGAGGAGT
    CTGGATGAGA TCCACCAGAA CCGCCATCAT TCCTACTCTC CCTCCCATTA CCACGACTCC
    CACCAGGAAC ATCGATCCGG AGACTCGGAC TATGAATATT CAGAGGACAG CGAGGTCCTG
    GAGATGCACA GATCAATCCG AGGTGGGAGT GCTGAGTGCC TGCATACAAA CAGGAAATTA
    GCTAGACACT GTAACACACT TCCCCCAAAG ATGCCATTGT TAGTGAACGG TATACATAAA
    GATATTTACA GCTCTACCCT TCCTGCATGC TTAAAGAGCA AAGCACCACG ACGAACAGAG
    AGGGATGAAG TTGTCCCTCT TAGTGCCATT CCACAGCGCA TTCTCAGGTT CACAGACGAG
    GTCAGCTCTG GTGATCTTCA GCCTTCATTA GACAGAGTAA GAAGTGCCAG CACAACTTGT
    TTGAGACCAG AGTCAAGTTT TCATTCACCT GAAAGTCATT TTCCATTTCA CAGGCATTCT
    GGCAATTTGG ATAGGCCAAG CTGTCAGATA GCTAACAAGA AAACCATTGA TGGTGACAGA
    CTTCAGCCCA CCTCTATCCT GAGAGGCAGC AGGAGGAGGA GAGAGACCCC CCTCAGGCCC
    TTTGGCCAGA CCAGCTTAGG GGGTTCCTGC CCAAACTCTC CTCGAGCTGA CAGAGATCTT
    CCACACGGAG GACATTATTC TCCAGCAGGG ACCACATCAT TAGGGCGCAG GGGCCGACAG
    CTGCCACAAC TCCCTGCAAA GAGCAGCAGT ATAGAACAAG GCCGTATACC TGAGGAGAAA
    CCTAACTCAG CTCTAGCAGT GGAGGAGCGG GCTCGTCAGT TGCAGATGAG AGTGCACTCG
    TATCGGCCGG GATCTTCTGC CAATTCTGCT CATGATCCAG AGGCAGAGCT TAAGAACAGG
    AGAGATATGT ACAAGGACCA GCGGAGGAGC TGTGACAACA TGTCTGCGAG GTCATCAGAC
    AGTGACATGA GCGACGTATC GGCCATCTCT CATGCCAGCA GTGCCTCACG GCTGAGTGCC
    ACCAGCTACA TGTCCATTCA GTCTGAAAGG CCACACGGGA GAATCAGGTC AAAGTCATTG
    GAAAGTTGTA AAGGAGAAGA AAAGAAAGAA AGGAGGATTT CGTGGGGGGT GAATGGGACA
    GACAACAGAA GGAGTTTGGG AAAGAGACGT TTCTCTGAGG AGCTTAAACG CAGACGGCAC
    ACGGTGGCTG GAGATGTGAG AGCCATCTTT GCATACCTCA CCTCATACTT TCCATGCTTG
    AATGCTGAGC CGGCACTGGA TCCCCCCAAA CCCAGAGCCC TATCTGCGCT TCAGCCTGTG
    GAAGAGAGAC CCACCTCCCA GCTCCCAATC CCCACCCTTC GTCCCACAGA TAGAGAGTTA
    GGGTGTAGAG GTGATGGAGA AAGTGCAGTG GGACAGTCCT GCAGTACACT GACTCCATCA
    GAGACAGATA CACGACAGCT CCTTCAAACC TCCAGGGGAC AGCAGAGGAC ATGGCTGCGC
    TGCAATAGTG CACTGCTGTG TCATAATACA GACTGTTTGA ACAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50140
    Clone ID Related Accession (Same CDS sequence) XM_009292234.2
    Accession Version XM_009292234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AACAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGGCC GTATACCTGA GGAGAAACCT
    AACTCAGCTC TAGCAGTGGA GGAGCGGGCT CGTCAGTTGC AGATGAGAGT GCACTCGTAT
    CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT GATCCAGAGG CAGAGCTTAA GAACAGGAGA
    GATATGTACA AGGACCAGCG GAGGAGCTGT GACAACATGT CTGCGAGGTC ATCAGACAGT
    GACATGAGCG ACGTATCGGC CATCTCTCAT GCCAGCAGTG CCTCACGGCT GAGTGCCACC
    AGCTACATGT CCATTCAGTC TGAAAGGCCA CACGGGAGAA TCAGGTCAAA GTCATTGGAA
    AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG AGGATTTCGT GGGGGGTGAA TGGGACAGAC
    AACAGAAGGA GTTTGGGAAA GAGACGTTTC TCTGAGGAGC TTAAACGCAG ACGGCACACG
    GTGGCTGGAG ATGTGAGAGC CATCTTTGCA TACCTCACCT CATACTTTCC ATGCTTGAAT
    GCTGAGCCGG CACTGGATCC CCCCAAACCC AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA
    GAGAGACCCA CCTCCCAGCT CCCAATCCCC ACCCTTCGTC CCACAGATAG AGAGTTAGGG
    TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA CAGTCCTGCA GTACACTGAC TCCATCAGAG
    ACAGATACAC GACAGCTCCT TCAAACCTCC AGGGGACAGC AGAGGACATG GCTGCGCTGC
    AATAGTGCAC TGCTGTGTCA TAATACAGAC TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290509.1
    CDS1261..5565
    Translation

    Target ORF information:

    RefSeq Version XM_009292234.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AACAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGACTT
    CAGCCCACCT CTATCCTGAG AGGCAGCAGG AGGAGGAGAG AGACCCCCCT CAGGCCCTTT
    GGCCAGACCA GCTTAGGGGG TTCCTGCCCA AACTCTCCTC GAGCTGACAG AGATCTTCCA
    CACGGAGGAC ATTATTCTCC AGCAGGGACC ACATCATTAG GGCGCAGGGG CCGACAGCTG
    CCACAACTCC CTGCAAAGAG CAGCAGTATA GAACAAGGCC GTATACCTGA GGAGAAACCT
    AACTCAGCTC TAGCAGTGGA GGAGCGGGCT CGTCAGTTGC AGATGAGAGT GCACTCGTAT
    CGGCCGGGAT CTTCTGCCAA TTCTGCTCAT GATCCAGAGG CAGAGCTTAA GAACAGGAGA
    GATATGTACA AGGACCAGCG GAGGAGCTGT GACAACATGT CTGCGAGGTC ATCAGACAGT
    GACATGAGCG ACGTATCGGC CATCTCTCAT GCCAGCAGTG CCTCACGGCT GAGTGCCACC
    AGCTACATGT CCATTCAGTC TGAAAGGCCA CACGGGAGAA TCAGGTCAAA GTCATTGGAA
    AGTTGTAAAG GAGAAGAAAA GAAAGAAAGG AGGATTTCGT GGGGGGTGAA TGGGACAGAC
    AACAGAAGGA GTTTGGGAAA GAGACGTTTC TCTGAGGAGC TTAAACGCAG ACGGCACACG
    GTGGCTGGAG ATGTGAGAGC CATCTTTGCA TACCTCACCT CATACTTTCC ATGCTTGAAT
    GCTGAGCCGG CACTGGATCC CCCCAAACCC AGAGCCCTAT CTGCGCTTCA GCCTGTGGAA
    GAGAGACCCA CCTCCCAGCT CCCAATCCCC ACCCTTCGTC CCACAGATAG AGAGTTAGGG
    TGTAGAGGTG ATGGAGAAAG TGCAGTGGGA CAGTCCTGCA GTACACTGAC TCCATCAGAG
    ACAGATACAC GACAGCTCCT TCAAACCTCC AGGGGACAGC AGAGGACATG GCTGCGCTGC
    AATAGTGCAC TGCTGTGTCA TAATACAGAC TGTTTGAACA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50141
    Clone ID Related Accession (Same CDS sequence) XM_009292239.2
    Accession Version XM_009292239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAGGTTT GGGATTTTGA AAGGAAATCG CAGATCATGG CTGTTCGCCA CAAACAGAGG 
    ACACTGCACC AGCAGTTTGA GAGTTATAAG GAGCAGGTGA GGAAGATCGG GGCAGAAACG
    AAGCGACAGC AGGCTGTCCA TAAAGATGAT GCACCTACCT GTGGCATCTG CCGTAAGACC
    AAGTTTGCAG ATGGCTGTGG GCATCTGTGC TCCTACTGCC AGACCAAGTT CTGTGCCCGC
    TGCGGGGGAA GAGTATCGTT GCGCTCAAAT AACGAGGATA AAGTGTGTCT CATAGGGAAA
    GACAGGGTGA TGTGGGTATG TAACCTGTGC AGGAAACAGC AGGAAATCCT AACTAAATCG
    GGAGAATGGT TTTCATCAGG GCCCGGGGGG AGGCCTCTTA GTGTGGGTTC TCCCCTCAGT
    GGCCCTGAGT TGGAGAAGGA GAGGAAGTTG CGCTCCAGAT CCCAGGTTCC GGGTGCCAAC
    GACACTCTGC TACCCAACGC TGGTCCCGGT GACCATCCAA AGGGAGCAGA TACAATGCCC
    GCCTCACGCT CCCGCAGTGA ACCACCAAGA GAAAAGAGGA GGCCAGTGTC CATGCATGAG
    CAGAATGGAA AAGTGATTGG ACGTGGAGAG AGAAGAAGAG CTCCAGCCAG GTTATCGTCG
    CAGGCCTCTG AAGACCGAGC CCCAGGTGAG AATCGAGATG GTAGACGACT AGAGAAAGAC
    CACTCACAGG AGGACGATCC CACCCACCCT GAGCGACGAA GAAGACAGGA GGAGGAAGAG
    CGAGAGCGGC AAAGACGAGA GGAAGAGTAC CAGACCCGTT ACCGTAGTGA CCCCAATCTA
    GCCCGCTACC CTGTTAAACC GCAGAAGGAG GAACAAGAAA TGCGCATGCA TGCCAAAGTC
    TCCAAAATGC GCCAGGAGCG TCATCACAGT GACCTGGCCA TCAATGAAGT GGGCCTGATG
    CAGGATGGTG GCGAAGCTGC TGGAAATCGC ATTAGCAGGC AACGAGTAGC TAGCAATGAG
    GACCGCAAGG CTCTTTTAGA AAACCATCGA GCTTATTCTG TGGACAGGAC CGTGGGGGGT
    GGTGTTGGGG GGCAAGGGTC CCTCAAACAG GGCCATGGTG GCCCTCAAAT GGGCCTTACT
    GGGCCTCCAG ACCTCAGGGA TGGACAGGAT TGGGGCCCAA AGGGGCATCT GGAACCTGGG
    TCTGCAGCTT TTCTGCACAG GGCCAAGCGG GAAAAAGCTG CAGAAATCAT GCGTAAAGAT
    TCTTCTCTCA GCTCAGACCA GTCTGAGTCA TTACGGCCAC CTCCACCAAG AGCCTACAGA
    CCCAAACGAG GCCTCAACAA AAGGCAGATG TCCATCAGTA GCTCAGAAGA AGAAGGAGGT
    TCCACGCCTG AATACACCAG CTGTGAGGAT GTGGAGATCG AAAGTGTTAG TGAAAAAGGT
    GACTGGGACT GTCATCCACT GGACCCCACT GTGTGGCATC ATCCAGTGAG TTGGCAGCCA
    TCCAAAGAGG GAGATCATTT GATTGGCCGT ATCACTTTAA GTAAGCGATC CACTATGCCA
    CGAGAAGCCG GTTCTCTACT GGGTCTGAAG GTTGTTGGTG GGAAAATGAC AGAGTCTGGT
    AGACTGGGGG CCTTTATCAC CAAAGTAAAG AAAGGAAGTT TGGCTGACAT TGTCGGGCAT
    TTACGAGCAG GCGATGAGGT GTTGCAGTGG AATGGAAAAG CGTTGCCTGG AGCAACTAAA
    AAGGAAGTGT ATAATATTAT TCTGGAGTCT CAGTCTGCAC CTCAAGTGGA AATAGTTGTT
    TCCAGACCGA TTGGATGTAT TCAACGAATT CTATCCAAAA ACTTCTTGAA AAAGGTCTAT
    AGAGCATATC AAGAAACGCC AAGACTTCCA GGGACATCAC ACCCCCCTCT AGAATCAACT
    GGTTCGAGTT CTATAGATGA ATCTCAAAAG ATGGATCGTC CATCCATCTC TGTAATGTCT
    CCGACTAGTC CCGGGATGTT GAGAGACCTC CCTCTTGTAC TGCCAGGCCA GCTGTCGGTG
    AAATTATGGT ATGATAAAGT GGGCCATCAG CTTATTGTCA ATGTTCTACA AGCACGAGAA
    CTGCCCCCTC GACCTGATGG ACGACCCAGG AATCCCTACG TCAAAATGTA TTTTCTTCCT
    GATAGAAGTG ACAAAAGCAA AAGAAGGACA AAAACGGTGA AGAAGAGTGC AGAGCCAAAG
    TGGAACCAGA CGTTTCTGTA TTCTCACGTC CATCGCAGAG ACTTCAGAGA ACGCATGCTG
    GAGATCACAG TTTGGGATCA GCCCAGAGCC CAGGAAGAAG AGAGTGATTT TCTGGGAGAG
    ATTCTGATTG AGCTGGAAAC AGCTCTGTTG GATGATCAGG CACACTGGTA CAAATTTCAG
    ACCCATGACG TGTCATCGCT TCCGTTGCCC CAACCGTCCC CTTGCCTCCC GCGCAGACAT
    GTTCATGGAG ACAGTCCTAG TAAAAAACTG CAAAGATCTC ATCGCATCAT TGAAACAGAT
    TATGATGATG GTATAACAGT AGTGTCCTCA GCAGCAGAAA GGACCTCCAG AGACAGAGAG
    AGGTCAAGCA CATTGACTGT ACCTGAGAGA CAGGCGGCCA TACAGCATCG CTCGCGGTCA
    GTTTCTCCGC ACAGGGAAGA CCAGTGTAGA GCCCGCTCTC GACCCGCACA CATCCCTATG
    CAGAGGAGTC TGGATGAGAT CCACCAGAAC CGCCATCATT CCTACTCTCC CTCCCATTAC
    CACGACTCCC ACCAGGAACA TCGATCCGGA GACTCGGACT ATGAATATTC AGAGGACAGC
    GAGGTCCTGG AGATGCACAG ATCAATCCGA GGTGGGAGTG CTGAGTGCCT GCATACAAAC
    AGGAAATTAG CTAGACACTG TAACACACTT CCCCCAAAGA TGCCATTGTT AGTGAACGGT
    ATACATAAAG ATATTTACAG CTCTACCCTT CCTGCATGCT TAAAGAGCAA AGCACCACGA
    CGAACAGAGA GGGATGAAGT TGTCCCTCTT AGTGCCATTC CACAGCGCAT TCTCAGGTTC
    ACAGACGAGG TCAGCTCTGG TGATCTTCAG CCTTCATTAG ACAGAGTAAG AAGTGCCAGC
    ACAACTTGTT TGAGACCAGA GTCAAGTTTT CATTCACCTG AAAGTCATTT TCCATTTCAC
    AGGCATTCTG GCAATTTGGA TAGGCCAAGC TGTCAGATAG CTAACAAGAA AACCATTGAT
    GGTGACAGAC TTCAGCCCAC CTCTATCCTG AGAGGCAGCA GGAGGAGGAG AGAGACCCCC
    CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG GGTTCCTGCC CAAACTCTCC TCGAGCTGAC
    AGAGATCTTC CACACGGAGG ACATTATTCT CCAGCAGGGA CCACATCATT AGGGCGCAGG
    GGCCGACAGC TGCCACAACT CCCTGCAAAG AGCAGCAGTA TAGAACAAGG CCGTATACCT
    GAGGAGAAAC CTAACTCAGC TCTAGCAGTG GAGGAGCGGG CTCGTCAGTT GCAGATGAGA
    GTGCACTCGT ATCGGCCGGG ATCTTCTGCC AATTCTGCTC ATGATCCAGA GGCAGAGCTT
    AAGAACAGGA GAGATATGTA CAAGGACCAG CGGAGGAGCT GTGACAACAT GTCTGCGAGG
    TCATCAGACA GTGACATGAG CGACGTATCG GCCATCTCTC ATGCCAGCAG TGCCTCACGG
    CTGAGTGCCA CCAGCTACAT GTCCATTCAG TCTGAAAGGC CACACGGGAG AATCAGGTCA
    AAGTCATTGG AAAGTTGTAA AGGAGAAGAA AAGAAAGAAA GGAGGATTTC GTGGGGGGTG
    AATGGGACAG ACAACAGAAG GAGTTTGGGA AAGAGACGTT TCTCTGAGGA GCTTAAACGC
    AGACGGCACA CGGTGGCTGG AGATGTGAGA GCCATCTTTG CATACCTCAC CTCATACTTT
    CCATGCTTGA ATGCTGAGCC GGCACTGGAT CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT
    CAGCCTGTGG AAGAGAGACC CACCTCCCAG CTCCCAATCC CCACCCTTCG TCCCACAGAT
    AGAGAGTTAG GGTGTAGAGG TGATGGAGAA AGTGCAGTGG GACAGTCCTG CAGTACACTG
    ACTCCATCAG AGACAGATAC ACGACAGCTC CTTCAAACCT CCAGGGGACA GCAGAGGACA
    TGGCTGCGCT GCAATAGTGC ACTGCTGTGT CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290514.1
    CDS147..4403
    Translation

    Target ORF information:

    RefSeq Version XM_009292239.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAGGTTT GGGATTTTGA AAGGAAATCG CAGATCATGG CTGTTCGCCA CAAACAGAGG 
    ACACTGCACC AGCAGTTTGA GAGTTATAAG GAGCAGGTGA GGAAGATCGG GGCAGAAACG
    AAGCGACAGC AGGCTGTCCA TAAAGATGAT GCACCTACCT GTGGCATCTG CCGTAAGACC
    AAGTTTGCAG ATGGCTGTGG GCATCTGTGC TCCTACTGCC AGACCAAGTT CTGTGCCCGC
    TGCGGGGGAA GAGTATCGTT GCGCTCAAAT AACGAGGATA AAGTGTGTCT CATAGGGAAA
    GACAGGGTGA TGTGGGTATG TAACCTGTGC AGGAAACAGC AGGAAATCCT AACTAAATCG
    GGAGAATGGT TTTCATCAGG GCCCGGGGGG AGGCCTCTTA GTGTGGGTTC TCCCCTCAGT
    GGCCCTGAGT TGGAGAAGGA GAGGAAGTTG CGCTCCAGAT CCCAGGTTCC GGGTGCCAAC
    GACACTCTGC TACCCAACGC TGGTCCCGGT GACCATCCAA AGGGAGCAGA TACAATGCCC
    GCCTCACGCT CCCGCAGTGA ACCACCAAGA GAAAAGAGGA GGCCAGTGTC CATGCATGAG
    CAGAATGGAA AAGTGATTGG ACGTGGAGAG AGAAGAAGAG CTCCAGCCAG GTTATCGTCG
    CAGGCCTCTG AAGACCGAGC CCCAGGTGAG AATCGAGATG GTAGACGACT AGAGAAAGAC
    CACTCACAGG AGGACGATCC CACCCACCCT GAGCGACGAA GAAGACAGGA GGAGGAAGAG
    CGAGAGCGGC AAAGACGAGA GGAAGAGTAC CAGACCCGTT ACCGTAGTGA CCCCAATCTA
    GCCCGCTACC CTGTTAAACC GCAGAAGGAG GAACAAGAAA TGCGCATGCA TGCCAAAGTC
    TCCAAAATGC GCCAGGAGCG TCATCACAGT GACCTGGCCA TCAATGAAGT GGGCCTGATG
    CAGGATGGTG GCGAAGCTGC TGGAAATCGC ATTAGCAGGC AACGAGTAGC TAGCAATGAG
    GACCGCAAGG CTCTTTTAGA AAACCATCGA GCTTATTCTG TGGACAGGAC CGTGGGGGGT
    GGTGTTGGGG GGCAAGGGTC CCTCAAACAG GGCCATGGTG GCCCTCAAAT GGGCCTTACT
    GGGCCTCCAG ACCTCAGGGA TGGACAGGAT TGGGGCCCAA AGGGGCATCT GGAACCTGGG
    TCTGCAGCTT TTCTGCACAG GGCCAAGCGG GAAAAAGCTG CAGAAATCAT GCGTAAAGAT
    TCTTCTCTCA GCTCAGACCA GTCTGAGTCA TTACGGCCAC CTCCACCAAG AGCCTACAGA
    CCCAAACGAG GCCTCAACAA AAGGCAGATG TCCATCAGTA GCTCAGAAGA AGAAGGAGGT
    TCCACGCCTG AATACACCAG CTGTGAGGAT GTGGAGATCG AAAGTGTTAG TGAAAAAGGT
    GACTGGGACT GTCATCCACT GGACCCCACT GTGTGGCATC ATCCAGTGAG TTGGCAGCCA
    TCCAAAGAGG GAGATCATTT GATTGGCCGT ATCACTTTAA GTAAGCGATC CACTATGCCA
    CGAGAAGCCG GTTCTCTACT GGGTCTGAAG GTTGTTGGTG GGAAAATGAC AGAGTCTGGT
    AGACTGGGGG CCTTTATCAC CAAAGTAAAG AAAGGAAGTT TGGCTGACAT TGTCGGGCAT
    TTACGAGCAG GCGATGAGGT GTTGCAGTGG AATGGAAAAG CGTTGCCTGG AGCAACTAAA
    AAGGAAGTGT ATAATATTAT TCTGGAGTCT CAGTCTGCAC CTCAAGTGGA AATAGTTGTT
    TCCAGACCGA TTGGATGTAT TCAACGAATT CTATCCAAAA ACTTCTTGAA AAAGGTCTAT
    AGAGCATATC AAGAAACGCC AAGACTTCCA GGGACATCAC ACCCCCCTCT AGAATCAACT
    GGTTCGAGTT CTATAGATGA ATCTCAAAAG ATGGATCGTC CATCCATCTC TGTAATGTCT
    CCGACTAGTC CCGGGATGTT GAGAGACCTC CCTCTTGTAC TGCCAGGCCA GCTGTCGGTG
    AAATTATGGT ATGATAAAGT GGGCCATCAG CTTATTGTCA ATGTTCTACA AGCACGAGAA
    CTGCCCCCTC GACCTGATGG ACGACCCAGG AATCCCTACG TCAAAATGTA TTTTCTTCCT
    GATAGAAGTG ACAAAAGCAA AAGAAGGACA AAAACGGTGA AGAAGAGTGC AGAGCCAAAG
    TGGAACCAGA CGTTTCTGTA TTCTCACGTC CATCGCAGAG ACTTCAGAGA ACGCATGCTG
    GAGATCACAG TTTGGGATCA GCCCAGAGCC CAGGAAGAAG AGAGTGATTT TCTGGGAGAG
    ATTCTGATTG AGCTGGAAAC AGCTCTGTTG GATGATCAGG CACACTGGTA CAAATTTCAG
    ACCCATGACG TGTCATCGCT TCCGTTGCCC CAACCGTCCC CTTGCCTCCC GCGCAGACAT
    GTTCATGGAG ACAGTCCTAG TAAAAAACTG CAAAGATCTC ATCGCATCAT TGAAACAGAT
    TATGATGATG GTATAACAGT AGTGTCCTCA GCAGCAGAAA GGACCTCCAG AGACAGAGAG
    AGGTCAAGCA CATTGACTGT ACCTGAGAGA CAGGCGGCCA TACAGCATCG CTCGCGGTCA
    GTTTCTCCGC ACAGGGAAGA CCAGTGTAGA GCCCGCTCTC GACCCGCACA CATCCCTATG
    CAGAGGAGTC TGGATGAGAT CCACCAGAAC CGCCATCATT CCTACTCTCC CTCCCATTAC
    CACGACTCCC ACCAGGAACA TCGATCCGGA GACTCGGACT ATGAATATTC AGAGGACAGC
    GAGGTCCTGG AGATGCACAG ATCAATCCGA GGTGGGAGTG CTGAGTGCCT GCATACAAAC
    AGGAAATTAG CTAGACACTG TAACACACTT CCCCCAAAGA TGCCATTGTT AGTGAACGGT
    ATACATAAAG ATATTTACAG CTCTACCCTT CCTGCATGCT TAAAGAGCAA AGCACCACGA
    CGAACAGAGA GGGATGAAGT TGTCCCTCTT AGTGCCATTC CACAGCGCAT TCTCAGGTTC
    ACAGACGAGG TCAGCTCTGG TGATCTTCAG CCTTCATTAG ACAGAGTAAG AAGTGCCAGC
    ACAACTTGTT TGAGACCAGA GTCAAGTTTT CATTCACCTG AAAGTCATTT TCCATTTCAC
    AGGCATTCTG GCAATTTGGA TAGGCCAAGC TGTCAGATAG CTAACAAGAA AACCATTGAT
    GGTGACAGAC TTCAGCCCAC CTCTATCCTG AGAGGCAGCA GGAGGAGGAG AGAGACCCCC
    CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG GGTTCCTGCC CAAACTCTCC TCGAGCTGAC
    AGAGATCTTC CACACGGAGG ACATTATTCT CCAGCAGGGA CCACATCATT AGGGCGCAGG
    GGCCGACAGC TGCCACAACT CCCTGCAAAG AGCAGCAGTA TAGAACAAGG CCGTATACCT
    GAGGAGAAAC CTAACTCAGC TCTAGCAGTG GAGGAGCGGG CTCGTCAGTT GCAGATGAGA
    GTGCACTCGT ATCGGCCGGG ATCTTCTGCC AATTCTGCTC ATGATCCAGA GGCAGAGCTT
    AAGAACAGGA GAGATATGTA CAAGGACCAG CGGAGGAGCT GTGACAACAT GTCTGCGAGG
    TCATCAGACA GTGACATGAG CGACGTATCG GCCATCTCTC ATGCCAGCAG TGCCTCACGG
    CTGAGTGCCA CCAGCTACAT GTCCATTCAG TCTGAAAGGC CACACGGGAG AATCAGGTCA
    AAGTCATTGG AAAGTTGTAA AGGAGAAGAA AAGAAAGAAA GGAGGATTTC GTGGGGGGTG
    AATGGGACAG ACAACAGAAG GAGTTTGGGA AAGAGACGTT TCTCTGAGGA GCTTAAACGC
    AGACGGCACA CGGTGGCTGG AGATGTGAGA GCCATCTTTG CATACCTCAC CTCATACTTT
    CCATGCTTGA ATGCTGAGCC GGCACTGGAT CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT
    CAGCCTGTGG AAGAGAGACC CACCTCCCAG CTCCCAATCC CCACCCTTCG TCCCACAGAT
    AGAGAGTTAG GGTGTAGAGG TGATGGAGAA AGTGCAGTGG GACAGTCCTG CAGTACACTG
    ACTCCATCAG AGACAGATAC ACGACAGCTC CTTCAAACCT CCAGGGGACA GCAGAGGACA
    TGGCTGCGCT GCAATAGTGC ACTGCTGTGT CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50142
    Clone ID Related Accession (Same CDS sequence) XM_009292243.2
    Accession Version XM_009292243.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGAGAT
    CTTCCACACG GAGGACATTA TTCTCCAGCA GGGACCACAT CATTAGGGCG CAGGGGCCGA
    CAGCTGCCAC AACTCCCTGC AAAGAGCAGC AGTATAGAAC AAGGCCGTAT ACCTGAGGAG
    AAACCTAACT CAGCTCTAGC AGTGGAGGAG CGGGCTCGTC AGTTGCAGAT GAGAGTGCAC
    TCGTATCGGC CGGGATCTTC TGCCAATTCT GCTCATGATC CAGAGGCAGA GCTTAAGAAC
    AGGAGAGATA TGTACAAGGA CCAGCGGAGG AGCTGTGACA ACATGTCTGC GAGGTCATCA
    GACAGTGACA TGAGCGACGT ATCGGCCATC TCTCATGCCA GCAGTGCCTC ACGGCTGAGT
    GCCACCAGCT ACATGTCCAT TCAGTCTGAA AGGCCACACG GGAGAATCAG GTCAAAGTCA
    TTGGAAAGTT GTAAAGGAGA AGAAAAGAAA GAAAGGAGGA TTTCGTGGGG GGTGAATGGG
    ACAGACAACA GAAGGAGTTT GGGAAAGAGA CGTTTCTCTG AGGAGCTTAA ACGCAGACGG
    CACACGGTGG CTGGAGATGT GAGAGCCATC TTTGCATACC TCACCTCATA CTTTCCATGC
    TTGAATGCTG AGCCGGCACT GGATCCCCCC AAACCCAGAG CCCTATCTGC GCTTCAGCCT
    GTGGAAGAGA GACCCACCTC CCAGCTCCCA ATCCCCACCC TTCGTCCCAC AGATAGAGAG
    TTAGGGTGTA GAGGTGATGG AGAAAGTGCA GTGGGACAGT CCTGCAGTAC ACTGACTCCA
    TCAGAGACAG ATACACGACA GCTCCTTCAA ACCTCCAGGG GACAGCAGAG GACATGGCTG
    CGCTGCAATA GTGCACTGCT GTGTCATAAT ACAGACTGTT TGAACAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290518.1
    CDS1261..5511
    Translation

    Target ORF information:

    RefSeq Version XM_009292243.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGTCATTTTC CATTTCACAG GCATTCTGGC
    AATTTGGATA GGCCAAGCTG TCAGATAGCT AACAAGAAAA CCATTGATGG TGACAGAGAT
    CTTCCACACG GAGGACATTA TTCTCCAGCA GGGACCACAT CATTAGGGCG CAGGGGCCGA
    CAGCTGCCAC AACTCCCTGC AAAGAGCAGC AGTATAGAAC AAGGCCGTAT ACCTGAGGAG
    AAACCTAACT CAGCTCTAGC AGTGGAGGAG CGGGCTCGTC AGTTGCAGAT GAGAGTGCAC
    TCGTATCGGC CGGGATCTTC TGCCAATTCT GCTCATGATC CAGAGGCAGA GCTTAAGAAC
    AGGAGAGATA TGTACAAGGA CCAGCGGAGG AGCTGTGACA ACATGTCTGC GAGGTCATCA
    GACAGTGACA TGAGCGACGT ATCGGCCATC TCTCATGCCA GCAGTGCCTC ACGGCTGAGT
    GCCACCAGCT ACATGTCCAT TCAGTCTGAA AGGCCACACG GGAGAATCAG GTCAAAGTCA
    TTGGAAAGTT GTAAAGGAGA AGAAAAGAAA GAAAGGAGGA TTTCGTGGGG GGTGAATGGG
    ACAGACAACA GAAGGAGTTT GGGAAAGAGA CGTTTCTCTG AGGAGCTTAA ACGCAGACGG
    CACACGGTGG CTGGAGATGT GAGAGCCATC TTTGCATACC TCACCTCATA CTTTCCATGC
    TTGAATGCTG AGCCGGCACT GGATCCCCCC AAACCCAGAG CCCTATCTGC GCTTCAGCCT
    GTGGAAGAGA GACCCACCTC CCAGCTCCCA ATCCCCACCC TTCGTCCCAC AGATAGAGAG
    TTAGGGTGTA GAGGTGATGG AGAAAGTGCA GTGGGACAGT CCTGCAGTAC ACTGACTCCA
    TCAGAGACAG ATACACGACA GCTCCTTCAA ACCTCCAGGG GACAGCAGAG GACATGGCTG
    CGCTGCAATA GTGCACTGCT GTGTCATAAT ACAGACTGTT TGAACAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50143
    Clone ID Related Accession (Same CDS sequence) XM_009292246.2
    Accession Version XM_009292246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG TGATCTTCAG
    CCTTCATTAG ACAGAGTAAG AAGTGCCAGC ACAACTTGTT TGAGACCAGA GTCAAGTTTT
    CATTCACCTG AAAGTCATTT TCCATTTCAC AGGCATTCTG GCAATTTGGA TAGGCCAAGC
    TGTCAGATAG CTAACAAGAA AACCATTGAT GGTGACAGAC TTCAGCCCAC CTCTATCCTG
    AGAGGCAGCA GGAGGAGGAG AGAGACCCCC CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG
    GGTTCCTGCC CAAACTCTCC TCGAGCTGAC AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290521.1
    CDS1261..5427
    Translation

    Target ORF information:

    RefSeq Version XM_009292246.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG TGATCTTCAG
    CCTTCATTAG ACAGAGTAAG AAGTGCCAGC ACAACTTGTT TGAGACCAGA GTCAAGTTTT
    CATTCACCTG AAAGTCATTT TCCATTTCAC AGGCATTCTG GCAATTTGGA TAGGCCAAGC
    TGTCAGATAG CTAACAAGAA AACCATTGAT GGTGACAGAC TTCAGCCCAC CTCTATCCTG
    AGAGGCAGCA GGAGGAGGAG AGAGACCCCC CTCAGGCCCT TTGGCCAGAC CAGCTTAGGG
    GGTTCCTGCC CAAACTCTCC TCGAGCTGAC AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50144
    Clone ID Related Accession (Same CDS sequence) XM_009292247.2
    Accession Version XM_009292247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 1 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290522.1
    CDS1261..5427
    Translation

    Target ORF information:

    RefSeq Version XM_009292247.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 1b (rims1b), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCGCCT CGGTAGGGCC CCAAAGCGGC CCTCGCCCAC CCACCGTGCC GGCTCCCATG 
    CCTGATATGC CAGACTTGAG CCACCTCACG GAGGAGGAAA GGAAAGTCAT CATGGCAGTG
    ATGGTTCGGC AAAGGGAAGA AGAGGAGAAG GAACAAGCCA TGCTAAAGAC ACTGCACCAG
    CAGTTTGAGA GTTATAAGGA GCAGGTGAGG AAGATCGGGG CAGAAACGAA GCGACAGCAG
    GCTGTCCATA AAGATGATGC ACCTACCTGT GGCATCTGCC GTAAGACCAA GTTTGCAGAT
    GGCTGTGGGC ATCTGTGCTC CTACTGCCAG ACCAAGTTCT GTGCCCGCTG CGGGGGAAGA
    GTATCGTTGC GCTCAAATAA CGAGGATAAA GTGTGTCTCA TAGGGAAAGA CAGGGTGATG
    TGGGTATGTA ACCTGTGCAG GAAACAGCAG GAAATCCTAA CTAAATCGGG AGAATGGTTT
    TCATCAGGGC CCGGGGGGAG GCCTCTTAGT GTGGGTTCTC CCCTCAGTGG CCCTGAGTTG
    GAGAAGGAGA GGAAGTTGCG CTCCAGATCC CAGGTTCCGG GTGCCAACGA CACTCTGCTA
    CCCAACGCTG GTCCCGGTGA CCATCCAAAG GGAGCAGATA CAATGCCCGC CTCACGCTCC
    CGCAGTGAAC CACCAAGAGA AAAGAGGAGG CCAGTGTCCA TGCATGAGCA GAATGGAAAA
    GTGATTGGAC GTGGAGAGAG AAGAAGAGCT CCAGCCAGGT TATCGTCGCA GGCCTCTGAA
    GACCGAGCCC CAGGTGAGAA TCGAGATGGT AGACGACTAG AGAAAGACCA CTCACAGGAG
    GACGATCCCA CCCACCCTGA GCGACGAAGA AGACAGGAGG AGGAAGAGCG AGAGCGGCAA
    AGACGAGAGG AAGAGTACCA GACCCGTTAC CGTAGTGACC CCAATCTAGC CCGCTACCCT
    GTTAAACCGC AGAAGGAGGA ACAAGAAATG CGCATGCATG CCAAAGTCTC CAAAATGCGC
    CAGGAGCGTC ATCACAGTGA CCTGGCCATC AATGAAGTGG GCCTGATGCA GGATGGTGGC
    GAAGCTGCTG GAAATCGCAT TAGCAGGCAA CGAGTAGCTA GCAATGAGGA CCGCAAGGCT
    CTTTTAGAAA ACCATCGAGC TTATTCTGTG GACAGGACCG TGGGGGGTGG TGTTGGGGGG
    CAAGGGTCCC TCAAACAGGG CCATGGTGGC CCTCAAATGG GCCTTACTGG GCCTCCAGAC
    CTCAGGGATG GACAGGATTG GGGCCCAAAG GGGCATCTGG AACCTGGGTC TGCAGCTTTT
    CTGCACAGGG CCAAGCGGGA AAAAGCTGCA GAAATCATGC GTAAAGATTC TTCTCTCAGC
    TCAGACCAGT CTGAGTCATT ACGGCCACCT CCACCAAGAG CCTACAGACC CAAACGAGGC
    CTCAACAAAA GGCAGATGTC CATCAGTAGC TCAGAAGAAG AAGGAGGTTC CACGCCTGAA
    TACACCAGCT GTGAGGATGT GGAGATCGAA AGTGTTAGTG AAAAAGGTGA CTGGGACTGT
    CATCCACTGG ACCCCACTGT GTGGCATCAT CCAGTGAGTT GGCAGCCATC CAAAGAGGGA
    GATCATTTGA TTGGCCGTAT CACTTTAAGT AAGCGATCCA CTATGCCACG AGAAGCCGGT
    TCTCTACTGG GTCTGAAGGT TGTTGGTGGG AAAATGACAG AGTCTGGTAG ACTGGGGGCC
    TTTATCACCA AAGTAAAGAA AGGAAGTTTG GCTGACATTG TCGGGCATTT ACGAGCAGGC
    GATGAGGTGT TGCAGTGGAA TGGAAAAGCG TTGCCTGGAG CAACTAAAAA GGAAGTGTAT
    AATATTATTC TGGAGTCTCA GTCTGCACCT CAAGTGGAAA TAGTTGTTTC CAGACCGATT
    GGATGTATTC AACGAATTCT ATCCAAAAAC TTCTTGAAAA AGGTCTATAG AGCATATCAA
    GAAACGCCAA GACTTCCAGG GACATCACAC CCCCCTCTAG AATCAACTGG TTCGAGTTCT
    ATAGATGAAT CTCAAAAGAT GGATCGTCCA TCCATCTCTG TAATGTCTCC GACTAGTCCC
    GGGATGTTGA GAGACCTCCC TCTTGTACTG CCAGGCCAGC TGTCGGTGAA ATTATGGTAT
    GATAAAGTGG GCCATCAGCT TATTGTCAAT GTTCTACAAG CACGAGAACT GCCCCCTCGA
    CCTGATGGAC GACCCAGGAA TCCCTACGTC AAAATGTATT TTCTTCCTGA TAGAAGTGAC
    AAAAGCAAAA GAAGGACAAA AACGGTGAAG AAGAGTGCAG AGCCAAAGTG GAACCAGACG
    TTTCTGTATT CTCACGTCCA TCGCAGAGAC TTCAGAGAAC GCATGCTGGA GATCACAGTT
    TGGGATCAGC CCAGAGCCCA GGAAGAAGAG AGTGATTTTC TGGGAGAGAT TCTGATTGAG
    CTGGAAACAG CTCTGTTGGA TGATCAGGCA CACTGGTACA AATTTCAGAC CCATGACGTG
    TCATCGCTTC CGTTGCCCCA ACCGTCCCCT TGCCTCCCGC GCAGACATGT TCATGGAGAC
    AGTCCTAGTA AAAAACTGCA AAGATCTCAT CGCATCATTG AAACAGATTA TGATGATGGT
    ATAACAGTAG TGTCCTCAGC AGCAGAAAGG ACCTCCAGAG ACAGAGAGAG GTCAAGCACA
    TTGACTGTAC CTGAGAGACA GGCGGCCATA CAGCATCGCT CGCGGTCAGT TTCTCCGCAC
    AGGGAAGACC AGTGTAGAGC CCGCTCTCGA CCCGCACACA TCCCTATGCA GAGGAGTCTG
    GATGAGATCC ACCAGAACCG CCATCATTCC TACTCTCCCT CCCATTACCA CGACTCCCAC
    CAGGAACATC GATCCGGAGA CTCGGACTAT GAATATTCAG AGGACAGCGA GGTCCTGGAG
    ATGCACAGAT CAATCCGAGG TGGGAGTGCT GAGTGCCTGC ATACAAACAG GAAATTAGCT
    AGACACTGTA ACACACTTCC CCCAAAGATG CCATTGTTAG TGAACGGTAT ACATAAAGAT
    ATTTACAGCT CTACCCTTCC TGCATGCTTA AAGAGCAAAG CACCACGACG AACAGAGAGG
    GATGAAGTTG TCCCTCTTAG TGCCATTCCA CAGCGCATTC TCAGGTTCAC AGACGAGGTC
    AGCTCTGGTG ATCTTCAGCC TTCATTAGAC AGAGTAAGAA GTGCCAGCAC AACTTGTTTG
    AGACCAGAGT CAAGTTTTCA TTCACCTGAA AGAGATCTTC CACACGGAGG ACATTATTCT
    CCAGCAGGGA CCACATCATT AGGGCGCAGG GGCCGACAGC TGCCACAACT CCCTGCAAAG
    AGCAGCAGTA TAGAACAAGG CCGTATACCT GAGGAGAAAC CTAACTCAGC TCTAGCAGTG
    GAGGAGCGGG CTCGTCAGTT GCAGATGAGA GTGCACTCGT ATCGGCCGGG ATCTTCTGCC
    AATTCTGCTC ATGATCCAGA GGCAGAGCTT AAGAACAGGA GAGATATGTA CAAGGACCAG
    CGGAGGAGCT GTGACAACAT GTCTGCGAGG TCATCAGACA GTGACATGAG CGACGTATCG
    GCCATCTCTC ATGCCAGCAG TGCCTCACGG CTGAGTGCCA CCAGCTACAT GTCCATTCAG
    TCTGAAAGGC CACACGGGAG AATCAGGTCA AAGTCATTGG AAAGTTGTAA AGGAGAAGAA
    AAGAAAGAAA GGAGGATTTC GTGGGGGGTG AATGGGACAG ACAACAGAAG GAGTTTGGGA
    AAGAGACGTT TCTCTGAGGA GCTTAAACGC AGACGGCACA CGGTGGCTGG AGATGTGAGA
    GCCATCTTTG CATACCTCAC CTCATACTTT CCATGCTTGA ATGCTGAGCC GGCACTGGAT
    CCCCCCAAAC CCAGAGCCCT ATCTGCGCTT CAGCCTGTGG AAGAGAGACC CACCTCCCAG
    CTCCCAATCC CCACCCTTCG TCCCACAGAT AGAGAGTTAG GGTGTAGAGG TGATGGAGAA
    AGTGCAGTGG GACAGTCCTG CAGTACACTG ACTCCATCAG AGACAGATAC ACGACAGCTC
    CTTCAAACCT CCAGGGGACA GCAGAGGACA TGGCTGCGCT GCAATAGTGC ACTGCTGTGT
    CATAATACAG ACTGTTTGAA CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.