THBS2A cDNA ORF clone, Danio rerio(Zebrafish)

The following THBS2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa54766 XM_017358840.1
Latest version!
Danio rerio thrombospondin 2a (thbs2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa18999 XM_009307490.3
Latest version!
Danio rerio thrombospondin 2a (thbs2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa18999 XM_009307490.2 Danio rerio thrombospondin 2a (thbs2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa54766
    Clone ID Related Accession (Same CDS sequence) XM_017358840.1
    Accession Version XM_017358840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product thrombospondin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGTGT ATTTTGAAGG AGTTATGATA CTCAGGAGTA ACCTCTGCTT GTGGCCTTTG 
    CTGTTACTAT GTATCAGAGC ACTACCTCAA GATGGCCTTG TGGAAGATGA ATCAATCTTC
    GATCTCTTCA AAATCAGCGG CATTAGCAGA AAAACGATTG GAGCCAAGCT CTTCAAGGGC
    CACGACTGGG ACTCACCGGC ATATCGTTTC ATCCGCTTTG ACCACATCCC AGCTGTGAGC
    ACCCCGGCGC TCCATCAGAT CCTTAAACAG GTCCAGAACA ATGAGGGCTT TGTGTTTGTG
    GCCACCATGA GACAAGACAA GGGCTCCAGG GGCACCTTAC TTGGAGTGGA GGGTCCCGGA
    GGGCTCCGTC AGCTTGAGAT AGTGTCCAAT GGTCGCGCCA ACACACTGGA TCTGGTCTAC
    ATGGTAAAGG GATCCCAGAA CGTGGTGTCC TTCGAGGATG TGGACCTGTC TGATTCGCAG
    TGGAAGAACA TCTCGCTGTA CGTTCATGGG GAAAACGCTC ACCTGTATGT GGGCTGCAGC
    CTCATAGACA GCGTGATCCT GGATGAGCCT TTCTATGAGC TCCTGCGTCC AGAGGGCAGC
    CAGATGTTTG TGGCCAAGGG ATCTATCAGG GAAAACCACT TCAGGGGCTT ACTGCAAAAT
    GTTCAGTTCC TTTTTGACAC TCCAATAGAA AACATCCTGA GAAACAAAGG CTGTGAGATT
    GCCAAACCTG AGGAAGTTAA CGTGGTTAAT GAGAGTACAG AATCTGTGTC TGTCGGTACA
    TCTATATCTA CCAACTTCAT TGGGGAGAAG GAGAAAATAG CATCAGACGT GTGCGAGCGC
    TCTTGTGAAG AGCTCAGTAA CATGGTCCAG GAGCTCAAAG GTCTTCGAAT CATCGTGGGC
    AATCTCATCG ATGGCTTGCA GAAAGTGACT GAGGAGAACA CCGTGATGAA GGAGGTGCTG
    GGAAACATGA AGAACATCAA AGACAAGCGC ATGTGCTGGC AAGACGGCCG TCTCTTTGAG
    GATAAAGAGG ACTGGGTTGT GGACAGCTGC ACCAAGTGTA CCTGTCAGGA ATCAAAGATT
    GTGTGCCATC AAATCACATG TCCACCAGTA GCATGTGCCA GCCCCACTTT CCTAGATGGC
    GAGTGCTGTC CACTGTGTCT GCCTAAAGAC AGCGAGGACG GCTGGTCACC TTGGTCTGAA
    TGGACAGAGT GTACGGTCAC ATGTGGGACG GGCACGCAAC AAAGAGGCAG GTCATGTGAT
    GCCACAAGTA ACCCCTGCAG TGGCCCATCT ATCCAAACCC GCAGATGCAG CTTGGGAAAA
    TGCGACAGTC GTGTTCGTCA AGATGGTGGT TGGAGCTTGT GGTCCCCCTG GTCATCTTGC
    TCTGTGACGT GTGGAGAAGG ACAAATCACA CGAATTCGCC ACTGCAACTC TCCTGTTCCT
    CAGCTGGGAG GAAAGGACTG TGAGGGCAGT GGGAGAGAGA CGCAAAAGTG TGAAGCTAAA
    CCCTGTCCAA TTGATGGAGC CTGGGGCCCC TGGTCTCCTT GGGCAGTCTG CTCTGTCACC
    TGTGGTGGAG GAACGAGGAC ACGAACACGT GTGTGTAACA GCCCTCGTCC ACAGTATGGA
    GGAAAGAAAT GCCCTGGAGA TTCCGTAGAC ACTAATGCCT GTAATAAACA GGACTGTCCT
    GTGGATGGTT GTCTGTCTAA CCCATGTTTC GGGGGTGTTG AATGCACAAG CTCTCCTGAC
    GGCTCGTGGG AGTGTGGTCC GTGCCCTCTC GGCTTCCGGG GCAATGGAAA AGTGTGTGAG
    GATGTCAATG AGTGTGACAT GGTCCCCGAT CTGTGCTATA AAGTGGGTGA TTCCCAGCGT
    TGTGTCAACA CAGACCCAGG ATTCCACTGC TTGCCTTGTC CTCCTCGATA CAAAGGCAAT
    CAGCCGTTTG GAATGGGAGT GGAGGCTGCC AAGCTAAATA AACAGGTGTG TGAGCAAGAA
    AACCCCTGCA AGGAGAAAAC CCACAACTGC CACAGGTCTG CTGAGTGCAT TTACCTTGGC
    CACTTCAGCG AACCCATGTT TAAGTGTGAG TGTAAGATCG GTTATGCTGG GGATGGACTC
    ATCTGTGGCG AGGACTCAGA CCTTGATGGC TGGCCTAATC AGAACCTGGT GTGTGGAGCC
    AACCAATCCT ACCACTGCAA AAAGGACAAT TGTCCCAAAC TTCCAAACTC AGGCCAAGAG
    GATTTTGATA AAGACGGTCA AGGTGACGCC TGCGACAAAG ACGATGATAA TGATGGAATC
    ATGGATGAGA CTGACAACTG TCCTCTACTG TATAATCCCA GGCAATTTGA CTATGATAAA
    GATCTTGTCG GTGACCGCTG TGATAACTGC CCATACGAGC ACAATCCTGC TCAGATTGAC
    ACAGACGGTA ATGGCGAAGG GGACGCCTGT GCTGTTGACA TTGATGGAGA TGAGGTTCTG
    AATGAGAATG ATAACTGTCC CTACATGTAC AACACTGACC AGAAGGACAC GGATATGGAT
    GGAGTGGGTG ACCAGTGTGA CAACTGCCCT CTGCTGCACA ACCCTGAGCA GACTGACTCA
    GATAATGACC GAGTTGGTGA CGAGTGCGAC AACAATCAAG ACATTGATGA GGACGGCCAC
    CAGAACAGCC TGGATAATTG TCCTTACATT CCCAACGCCA ACCAGGCCGA CCACGACAAA
    GATGGGAAAG GAGACGCTTG CGATTCCGAT GATGATAATG ACGGAGTCCC AGATGATAAG
    GACAACTGCA GACTGGTGCC CAACAAAGAC CAACGAGACT CTGATGGGGA TGGAAGAGGC
    GACGCCTGCA AAGACGATTT TGACAACGAC AGCATTCCAG ACTTTATGGA CGTGTGTCCT
    GAGAACGATG CCATCAGCAA CACAGACTTC CGCAAGTTTC AGATGGTGCA TTTGGATCCC
    AAAGGAACTG CGCAAATTGA TCCCAACTGG GTGGTCAGAC ATCAAGGCAA AGAGCTCGTT
    CAGACCGCCA ACTCGGACCC TGGCATTGCA GTGGGCTTCG ACGAATTCAA CGCTGTGGAT
    TTCAGCGGCA CTTTTTACGT CAACACAGAT AGAGATGATG ATTATGCTGG CTTTGTGTTC
    GGCTATCAGT CCAGCGGGCG CTTTTATGTG GTAATGTGGA AGCAGATAAC GCAGACATAC
    TGGGAGGACA ATCCTTCAAA AGCCATCGGC ATCTCTGGGG TTTCTCTGAA AGTGGTCAAC
    TCGACCACAG GCACAGGAGA GCACCTTCGC AATGCCCTGT GGCACACTGG GGACACACCT
    GGACAGGTTC GCACACTGTG GCACGACCCT AAGAACATCG GCTGGAAGGA TTACACCGCC
    TACAGGTGGC ACCTCATTCA CAGACCCAAG ACTGGATTCA TCAGAGTGGT GGTTTATGAG
    GGGAAACAGA TCATGGCAGA CTCAGGACCA GTTTATGATC AAACATTTGC AGGAGGGAGA
    TTAGGACTCT TCGTCTTCTC TCAGGAACTG GTTTATTTCT CTGATCTCAA GTATGAATGT
    CGAGATAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214329.1
    CDS99..3650
    Translation

    Target ORF information:

    RefSeq Version XM_017358840.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio thrombospondin 2a (thbs2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGTGT ATTTTGAAGG AGTTATGATA CTCAGGAGTA ACCTCTGCTT GTGGCCTTTG 
    CTGTTACTAT GTATCAGAGC ACTACCTCAA GATGGCCTTG TGGAAGATGA ATCAATCTTC
    GATCTCTTCA AAATCAGCGG CATTAGCAGA AAAACGATTG GAGCCAAGCT CTTCAAGGGC
    CACGACTGGG ACTCACCGGC ATATCGTTTC ATCCGCTTTG ACCACATCCC AGCTGTGAGC
    ACCCCGGCGC TCCATCAGAT CCTTAAACAG GTCCAGAACA ATGAGGGCTT TGTGTTTGTG
    GCCACCATGA GACAAGACAA GGGCTCCAGG GGCACCTTAC TTGGAGTGGA GGGTCCCGGA
    GGGCTCCGTC AGCTTGAGAT AGTGTCCAAT GGTCGCGCCA ACACACTGGA TCTGGTCTAC
    ATGGTAAAGG GATCCCAGAA CGTGGTGTCC TTCGAGGATG TGGACCTGTC TGATTCGCAG
    TGGAAGAACA TCTCGCTGTA CGTTCATGGG GAAAACGCTC ACCTGTATGT GGGCTGCAGC
    CTCATAGACA GCGTGATCCT GGATGAGCCT TTCTATGAGC TCCTGCGTCC AGAGGGCAGC
    CAGATGTTTG TGGCCAAGGG ATCTATCAGG GAAAACCACT TCAGGGGCTT ACTGCAAAAT
    GTTCAGTTCC TTTTTGACAC TCCAATAGAA AACATCCTGA GAAACAAAGG CTGTGAGATT
    GCCAAACCTG AGGAAGTTAA CGTGGTTAAT GAGAGTACAG AATCTGTGTC TGTCGGTACA
    TCTATATCTA CCAACTTCAT TGGGGAGAAG GAGAAAATAG CATCAGACGT GTGCGAGCGC
    TCTTGTGAAG AGCTCAGTAA CATGGTCCAG GAGCTCAAAG GTCTTCGAAT CATCGTGGGC
    AATCTCATCG ATGGCTTGCA GAAAGTGACT GAGGAGAACA CCGTGATGAA GGAGGTGCTG
    GGAAACATGA AGAACATCAA AGACAAGCGC ATGTGCTGGC AAGACGGCCG TCTCTTTGAG
    GATAAAGAGG ACTGGGTTGT GGACAGCTGC ACCAAGTGTA CCTGTCAGGA ATCAAAGATT
    GTGTGCCATC AAATCACATG TCCACCAGTA GCATGTGCCA GCCCCACTTT CCTAGATGGC
    GAGTGCTGTC CACTGTGTCT GCCTAAAGAC AGCGAGGACG GCTGGTCACC TTGGTCTGAA
    TGGACAGAGT GTACGGTCAC ATGTGGGACG GGCACGCAAC AAAGAGGCAG GTCATGTGAT
    GCCACAAGTA ACCCCTGCAG TGGCCCATCT ATCCAAACCC GCAGATGCAG CTTGGGAAAA
    TGCGACAGTC GTGTTCGTCA AGATGGTGGT TGGAGCTTGT GGTCCCCCTG GTCATCTTGC
    TCTGTGACGT GTGGAGAAGG ACAAATCACA CGAATTCGCC ACTGCAACTC TCCTGTTCCT
    CAGCTGGGAG GAAAGGACTG TGAGGGCAGT GGGAGAGAGA CGCAAAAGTG TGAAGCTAAA
    CCCTGTCCAA TTGATGGAGC CTGGGGCCCC TGGTCTCCTT GGGCAGTCTG CTCTGTCACC
    TGTGGTGGAG GAACGAGGAC ACGAACACGT GTGTGTAACA GCCCTCGTCC ACAGTATGGA
    GGAAAGAAAT GCCCTGGAGA TTCCGTAGAC ACTAATGCCT GTAATAAACA GGACTGTCCT
    GTGGATGGTT GTCTGTCTAA CCCATGTTTC GGGGGTGTTG AATGCACAAG CTCTCCTGAC
    GGCTCGTGGG AGTGTGGTCC GTGCCCTCTC GGCTTCCGGG GCAATGGAAA AGTGTGTGAG
    GATGTCAATG AGTGTGACAT GGTCCCCGAT CTGTGCTATA AAGTGGGTGA TTCCCAGCGT
    TGTGTCAACA CAGACCCAGG ATTCCACTGC TTGCCTTGTC CTCCTCGATA CAAAGGCAAT
    CAGCCGTTTG GAATGGGAGT GGAGGCTGCC AAGCTAAATA AACAGGTGTG TGAGCAAGAA
    AACCCCTGCA AGGAGAAAAC CCACAACTGC CACAGGTCTG CTGAGTGCAT TTACCTTGGC
    CACTTCAGCG AACCCATGTT TAAGTGTGAG TGTAAGATCG GTTATGCTGG GGATGGACTC
    ATCTGTGGCG AGGACTCAGA CCTTGATGGC TGGCCTAATC AGAACCTGGT GTGTGGAGCC
    AACCAATCCT ACCACTGCAA AAAGGACAAT TGTCCCAAAC TTCCAAACTC AGGCCAAGAG
    GATTTTGATA AAGACGGTCA AGGTGACGCC TGCGACAAAG ACGATGATAA TGATGGAATC
    ATGGATGAGA CTGACAACTG TCCTCTACTG TATAATCCCA GGCAATTTGA CTATGATAAA
    GATCTTGTCG GTGACCGCTG TGATAACTGC CCATACGAGC ACAATCCTGC TCAGATTGAC
    ACAGACGGTA ATGGCGAAGG GGACGCCTGT GCTGTTGACA TTGATGGAGA TGAGGTTCTG
    AATGAGAATG ATAACTGTCC CTACATGTAC AACACTGACC AGAAGGACAC GGATATGGAT
    GGAGTGGGTG ACCAGTGTGA CAACTGCCCT CTGCTGCACA ACCCTGAGCA GACTGACTCA
    GATAATGACC GAGTTGGTGA CGAGTGCGAC AACAATCAAG ACATTGATGA GGACGGCCAC
    CAGAACAGCC TGGATAATTG TCCTTACATT CCCAACGCCA ACCAGGCCGA CCACGACAAA
    GATGGGAAAG GAGACGCTTG CGATTCCGAT GATGATAATG ACGGAGTCCC AGATGATAAG
    GACAACTGCA GACTGGTGCC CAACAAAGAC CAACGAGACT CTGATGGGGA TGGAAGAGGC
    GACGCCTGCA AAGACGATTT TGACAACGAC AGCATTCCAG ACTTTATGGA CGTGTGTCCT
    GAGAACGATG CCATCAGCAA CACAGACTTC CGCAAGTTTC AGATGGTGCA TTTGGATCCC
    AAAGGAACTG CGCAAATTGA TCCCAACTGG GTGGTCAGAC ATCAAGGCAA AGAGCTCGTT
    CAGACCGCCA ACTCGGACCC TGGCATTGCA GTGGGCTTCG ACGAATTCAA CGCTGTGGAT
    TTCAGCGGCA CTTTTTACGT CAACACAGAT AGAGATGATG ATTATGCTGG CTTTGTGTTC
    GGCTATCAGT CCAGCGGGCG CTTTTATGTG GTAATGTGGA AGCAGATAAC GCAGACATAC
    TGGGAGGACA ATCCTTCAAA AGCCATCGGC ATCTCTGGGG TTTCTCTGAA AGTGGTCAAC
    TCGACCACAG GCACAGGAGA GCACCTTCGC AATGCCCTGT GGCACACTGG GGACACACCT
    GGACAGGTTC GCACACTGTG GCACGACCCT AAGAACATCG GCTGGAAGGA TTACACCGCC
    TACAGGTGGC ACCTCATTCA CAGACCCAAG ACTGGATTCA TCAGAGTGGT GGTTTATGAG
    GGGAAACAGA TCATGGCAGA CTCAGGACCA GTTTATGATC AAACATTTGC AGGAGGGAGA
    TTAGGACTCT TCGTCTTCTC TCAGGAACTG GTTTATTTCT CTGATCTCAA GTATGAATGT
    CGAGATAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18999
    Clone ID Related Accession (Same CDS sequence) XM_009307490.3 , XM_009307490.2
    Accession Version XM_009307490.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product thrombospondin-2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATACTCA GGAGTAACCT CTGCTTGTGG CCTTTGCTGT TACTATGTAT CAGAGCACTA 
    CCTCAAGATG GCCTTGTGGA AGATGAATCA ATCTTCGATC TCTTCAAAAT CAGCGGCATT
    AGCAGAAAAA CGATTGGAGC CAAGCTCTTC AAGGGCCACG ACTGGGACTC ACCGGCATAT
    CGTTTCATCC GCTTTGACCA CATCCCAGCT GTGAGCACCC CGGCGCTCCA TCAGATCCTT
    AAACAGGTCC AGAACAATGA GGGCTTTGTG TTTGTGGCCA CCATGAGACA AGACAAGGGC
    TCCAGGGGCA CCTTACTTGG AGTGGAGGGT CCCGGAGGGC TCCGTCAGCT TGAGATAGTG
    TCCAATGGTC GCGCCAACAC ACTGGATCTG GTCTACATGG TAAAGGGATC CCAGAACGTG
    GTGTCCTTCG AGGATGTGGA CCTGTCTGAT TCGCAGTGGA AGAACATCTC GCTGTACGTT
    CATGGGGAAA ACGCTCACCT GTATGTGGGC TGCAGCCTCA TAGACAGCGT GATCCTGGAT
    GAGCCTTTCT ATGAGCTCCT GCGTCCAGAG GGCAGCCAGA TGTTTGTGGC CAAGGGATCT
    ATCAGGGAAA ACCACTTCAG GGGCTTACTG CAAAATGTTC AGTTCCTTTT TGACACTCCA
    ATAGAAAACA TCCTGAGAAA CAAAGGCTGT GAGATTGCCA AACCTGAGGA AGTTAACGTG
    GTTAATGAGA GTACAGAATC TGTGTCTGTC GGTACATCTA TATCTACCAA CTTCATTGGG
    GAGAAGGAGA AAATAGCATC AGACGTGTGC GAGCGCTCTT GTGAAGAGCT CAGTAACATG
    GTCCAGGAGC TCAAAGGTCT TCGAATCATC GTGGGCAATC TCATCGATGG CTTGCAGAAA
    GTGACTGAGG AGAACACCGT GATGAAGGAG GTGCTGGGAA ACATGAAGAA CATCAAAGAC
    AAGCGCATGT GCTGGCAAGA CGGCCGTCTC TTTGAGGATA AAGAGGACTG GGTTGTGGAC
    AGCTGCACCA AGTGTACCTG TCAGGAATCA AAGATTGTGT GCCATCAAAT CACATGTCCA
    CCAGTAGCAT GTGCCAGCCC CACTTTCCTA GATGGCGAGT GCTGTCCACT GTGTCTGCCT
    AAAGACAGCG AGGACGGCTG GTCACCTTGG TCTGAATGGA CAGAGTGTAC GGTCACATGT
    GGGACGGGCA CGCAACAAAG AGGCAGGTCA TGTGATGCCA CAAGTAACCC CTGCAGTGGC
    CCATCTATCC AAACCCGCAG ATGCAGCTTG GGAAAATGCG ACAGTCGTGT TCGTCAAGAT
    GGTGGTTGGA GCTTGTGGTC CCCCTGGTCA TCTTGCTCTG TGACGTGTGG AGAAGGACAA
    ATCACACGAA TTCGCCACTG CAACTCTCCT GTTCCTCAGC TGGGAGGAAA GGACTGTGAG
    GGCAGTGGGA GAGAGACGCA AAAGTGTGAA GCTAAACCCT GTCCAATTGA TGGAGCCTGG
    GGCCCCTGGT CTCCTTGGGC AGTCTGCTCT GTCACCTGTG GTGGAGGAAC GAGGACACGA
    ACACGTGTGT GTAACAGCCC TCGTCCACAG TATGGAGGAA AGAAATGCCC TGGAGATTCC
    GTAGACACTA ATGCCTGTAA TAAACAGGAC TGTCCTGTGG ATGGTTGTCT GTCTAACCCA
    TGTTTCGGGG GTGTTGAATG CACAAGCTCT CCTGACGGCT CGTGGGAGTG TGGTCCGTGC
    CCTCTCGGCT TCCGGGGCAA TGGAAAAGTG TGTGAGGATG TCAATGAGTG TGACATGGTC
    CCCGATCTGT GCTATAAAGT GGGTGATTCC CAGCGTTGTG TCAACACAGA CCCAGGATTC
    CACTGCTTGC CTTGTCCTCC TCGATACAAA GGCAATCAGC CGTTTGGAAT GGGAGTGGAG
    GCTGCCAAGC TAAATAAACA GGTGTGTGAG CAAGAAAACC CCTGCAAGGA GAAAACCCAC
    AACTGCCACA GGTCTGCTGA GTGCATTTAC CTTGGCCACT TCAGCGAACC CATGTTTAAG
    TGTGAGTGTA AGATCGGTTA TGCTGGGGAT GGACTCATCT GTGGCGAGGA CTCAGACCTT
    GATGGCTGGC CTAATCAGAA CCTGGTGTGT GGAGCCAACC AATCCTACCA CTGCAAAAAG
    GACAATTGTC CCAAACTTCC AAACTCAGGC CAAGAGGATT TTGATAAAGA CGGTCAAGGT
    GACGCCTGCG ACAAAGACGA TGATAATGAT GGAATCATGG ATGAGACTGA CAACTGTCCT
    CTACTGTATA ATCCCAGGCA ATTTGACTAT GATAAAGATC TTGTCGGTGA CCGCTGTGAT
    AACTGCCCAT ACGAGCACAA TCCTGCTCAG ATTGACACAG ACGGTAATGG CGAAGGGGAC
    GCCTGTGCTG TTGACATTGA TGGAGATGAG GTTCTGAATG AGAATGATAA CTGTCCCTAC
    ATGTACAACA CTGACCAGAA GGACACGGAT ATGGATGGAG TGGGTGACCA GTGTGACAAC
    TGCCCTCTGC TGCACAACCC TGAGCAGACT GACTCAGATA ATGACCGAGT TGGTGACGAG
    TGCGACAACA ATCAAGACAT TGATGAGGAC GGCCACCAGA ACAGCCTGGA TAATTGTCCT
    TACATTCCCA ACGCCAACCA GGCCGACCAC GACAAAGATG GGAAAGGAGA CGCTTGCGAT
    TCCGATGATG ATAATGACGG AGTCCCAGAT GATAAGGACA ACTGCAGACT GGTGCCCAAC
    AAAGACCAAC GAGACTCTGA TGGGGATGGA AGAGGCGACG CCTGCAAAGA CGATTTTGAC
    AACGACAGCA TTCCAGACTT TATGGACGTG TGTCCTGAGA ACGATGCCAT CAGCAACACA
    GACTTCCGCA AGTTTCAGAT GGTGCATTTG GATCCCAAAG GAACTGCGCA AATTGATCCC
    AACTGGGTGG TCAGACATCA AGGCAAAGAG CTCGTTCAGA CCGCCAACTC GGACCCTGGC
    ATTGCAGTGG GCTTCGACGA ATTCAACGCT GTGGATTTCA GCGGCACTTT TTACGTCAAC
    ACAGATAGAG ATGATGATTA TGCTGGCTTT GTGTTCGGCT ATCAGTCCAG CGGGCGCTTT
    TATGTGGTAA TGTGGAAGCA GATAACGCAG ACATACTGGG AGGACAATCC TTCAAAAGCC
    ATCGGCATCT CTGGGGTTTC TCTGAAAGTG GTCAACTCGA CCACAGGCAC AGGAGAGCAC
    CTTCGCAATG CCCTGTGGCA CACTGGGGAC ACACCTGGAC AGGTTCGCAC ACTGTGGCAC
    GACCCTAAGA ACATCGGCTG GAAGGATTAC ACCGCCTACA GGTGGCACCT CATTCACAGA
    CCCAAGACTG GATTCATCAG AGTGGTGGTT TATGAGGGGA AACAGATCAT GGCAGACTCA
    GGACCAGTTT ATGATCAAAC ATTTGCAGGA GGGAGATTAG GACTCTTCGT CTTCTCTCAG
    GAACTGGTTT ATTTCTCTGA TCTCAAGTAT GAATGTCGAG ATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305765.1
    CDS821..4348
    Translation

    Target ORF information:

    RefSeq Version XM_009307490.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio thrombospondin 2a (thbs2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307490.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATACTCA GGAGTAACCT CTGCTTGTGG CCTTTGCTGT TACTATGTAT CAGAGCACTA 
    CCTCAAGATG GCCTTGTGGA AGATGAATCA ATCTTCGATC TCTTCAAAAT CAGCGGCATT
    AGCAGAAAAA CGATTGGAGC CAAGCTCTTC AAGGGCCACG ACTGGGACTC ACCGGCATAT
    CGTTTCATCC GCTTTGACCA CATCCCAGCT GTGAGCACCC CGGCGCTCCA TCAGATCCTT
    AAACAGGTCC AGAACAATGA GGGCTTTGTG TTTGTGGCCA CCATGAGACA AGACAAGGGC
    TCCAGGGGCA CCTTACTTGG AGTGGAGGGT CCCGGAGGGC TCCGTCAGCT TGAGATAGTG
    TCCAATGGTC GCGCCAACAC ACTGGATCTG GTCTACATGG TAAAGGGATC CCAGAACGTG
    GTGTCCTTCG AGGATGTGGA CCTGTCTGAT TCGCAGTGGA AGAACATCTC GCTGTACGTT
    CATGGGGAAA ACGCTCACCT GTATGTGGGC TGCAGCCTCA TAGACAGCGT GATCCTGGAT
    GAGCCTTTCT ATGAGCTCCT GCGTCCAGAG GGCAGCCAGA TGTTTGTGGC CAAGGGATCT
    ATCAGGGAAA ACCACTTCAG GGGCTTACTG CAAAATGTTC AGTTCCTTTT TGACACTCCA
    ATAGAAAACA TCCTGAGAAA CAAAGGCTGT GAGATTGCCA AACCTGAGGA AGTTAACGTG
    GTTAATGAGA GTACAGAATC TGTGTCTGTC GGTACATCTA TATCTACCAA CTTCATTGGG
    GAGAAGGAGA AAATAGCATC AGACGTGTGC GAGCGCTCTT GTGAAGAGCT CAGTAACATG
    GTCCAGGAGC TCAAAGGTCT TCGAATCATC GTGGGCAATC TCATCGATGG CTTGCAGAAA
    GTGACTGAGG AGAACACCGT GATGAAGGAG GTGCTGGGAA ACATGAAGAA CATCAAAGAC
    AAGCGCATGT GCTGGCAAGA CGGCCGTCTC TTTGAGGATA AAGAGGACTG GGTTGTGGAC
    AGCTGCACCA AGTGTACCTG TCAGGAATCA AAGATTGTGT GCCATCAAAT CACATGTCCA
    CCAGTAGCAT GTGCCAGCCC CACTTTCCTA GATGGCGAGT GCTGTCCACT GTGTCTGCCT
    AAAGACAGCG AGGACGGCTG GTCACCTTGG TCTGAATGGA CAGAGTGTAC GGTCACATGT
    GGGACGGGCA CGCAACAAAG AGGCAGGTCA TGTGATGCCA CAAGTAACCC CTGCAGTGGC
    CCATCTATCC AAACCCGCAG ATGCAGCTTG GGAAAATGCG ACAGTCGTGT TCGTCAAGAT
    GGTGGTTGGA GCTTGTGGTC CCCCTGGTCA TCTTGCTCTG TGACGTGTGG AGAAGGACAA
    ATCACACGAA TTCGCCACTG CAACTCTCCT GTTCCTCAGC TGGGAGGAAA GGACTGTGAG
    GGCAGTGGGA GAGAGACGCA AAAGTGTGAA GCTAAACCCT GTCCAATTGA TGGAGCCTGG
    GGCCCCTGGT CTCCTTGGGC AGTCTGCTCT GTCACCTGTG GTGGAGGAAC GAGGACACGA
    ACACGTGTGT GTAACAGCCC TCGTCCACAG TATGGAGGAA AGAAATGCCC TGGAGATTCC
    GTAGACACTA ATGCCTGTAA TAAACAGGAC TGTCCTGTGG ATGGTTGTCT GTCTAACCCA
    TGTTTCGGGG GTGTTGAATG CACAAGCTCT CCTGACGGCT CGTGGGAGTG TGGTCCGTGC
    CCTCTCGGCT TCCGGGGCAA TGGAAAAGTG TGTGAGGATG TCAATGAGTG TGACATGGTC
    CCCGATCTGT GCTATAAAGT GGGTGATTCC CAGCGTTGTG TCAACACAGA CCCAGGATTC
    CACTGCTTGC CTTGTCCTCC TCGATACAAA GGCAATCAGC CGTTTGGAAT GGGAGTGGAG
    GCTGCCAAGC TAAATAAACA GGTGTGTGAG CAAGAAAACC CCTGCAAGGA GAAAACCCAC
    AACTGCCACA GGTCTGCTGA GTGCATTTAC CTTGGCCACT TCAGCGAACC CATGTTTAAG
    TGTGAGTGTA AGATCGGTTA TGCTGGGGAT GGACTCATCT GTGGCGAGGA CTCAGACCTT
    GATGGCTGGC CTAATCAGAA CCTGGTGTGT GGAGCCAACC AATCCTACCA CTGCAAAAAG
    GACAATTGTC CCAAACTTCC AAACTCAGGC CAAGAGGATT TTGATAAAGA CGGTCAAGGT
    GACGCCTGCG ACAAAGACGA TGATAATGAT GGAATCATGG ATGAGACTGA CAACTGTCCT
    CTACTGTATA ATCCCAGGCA ATTTGACTAT GATAAAGATC TTGTCGGTGA CCGCTGTGAT
    AACTGCCCAT ACGAGCACAA TCCTGCTCAG ATTGACACAG ACGGTAATGG CGAAGGGGAC
    GCCTGTGCTG TTGACATTGA TGGAGATGAG GTTCTGAATG AGAATGATAA CTGTCCCTAC
    ATGTACAACA CTGACCAGAA GGACACGGAT ATGGATGGAG TGGGTGACCA GTGTGACAAC
    TGCCCTCTGC TGCACAACCC TGAGCAGACT GACTCAGATA ATGACCGAGT TGGTGACGAG
    TGCGACAACA ATCAAGACAT TGATGAGGAC GGCCACCAGA ACAGCCTGGA TAATTGTCCT
    TACATTCCCA ACGCCAACCA GGCCGACCAC GACAAAGATG GGAAAGGAGA CGCTTGCGAT
    TCCGATGATG ATAATGACGG AGTCCCAGAT GATAAGGACA ACTGCAGACT GGTGCCCAAC
    AAAGACCAAC GAGACTCTGA TGGGGATGGA AGAGGCGACG CCTGCAAAGA CGATTTTGAC
    AACGACAGCA TTCCAGACTT TATGGACGTG TGTCCTGAGA ACGATGCCAT CAGCAACACA
    GACTTCCGCA AGTTTCAGAT GGTGCATTTG GATCCCAAAG GAACTGCGCA AATTGATCCC
    AACTGGGTGG TCAGACATCA AGGCAAAGAG CTCGTTCAGA CCGCCAACTC GGACCCTGGC
    ATTGCAGTGG GCTTCGACGA ATTCAACGCT GTGGATTTCA GCGGCACTTT TTACGTCAAC
    ACAGATAGAG ATGATGATTA TGCTGGCTTT GTGTTCGGCT ATCAGTCCAG CGGGCGCTTT
    TATGTGGTAA TGTGGAAGCA GATAACGCAG ACATACTGGG AGGACAATCC TTCAAAAGCC
    ATCGGCATCT CTGGGGTTTC TCTGAAAGTG GTCAACTCGA CCACAGGCAC AGGAGAGCAC
    CTTCGCAATG CCCTGTGGCA CACTGGGGAC ACACCTGGAC AGGTTCGCAC ACTGTGGCAC
    GACCCTAAGA ACATCGGCTG GAAGGATTAC ACCGCCTACA GGTGGCACCT CATTCACAGA
    CCCAAGACTG GATTCATCAG AGTGGTGGTT TATGAGGGGA AACAGATCAT GGCAGACTCA
    GGACCAGTTT ATGATCAAAC ATTTGCAGGA GGGAGATTAG GACTCTTCGT CTTCTCTCAG
    GAACTGGTTT ATTTCTCTGA TCTCAAGTAT GAATGTCGAG ATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18999
    Clone ID Related Accession (Same CDS sequence) XM_009307490.3 , XM_009307490.2
    Accession Version XM_009307490.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product thrombospondin-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATACTCA GGAGTAACCT CTGCTTGTGG CCTTTGCTGT TACTATGTAT CAGAGCACTA 
    CCTCAAGATG GCCTTGTGGA AGATGAATCA ATCTTCGATC TCTTCAAAAT CAGCGGCATT
    AGCAGAAAAA CGATTGGAGC CAAGCTCTTC AAGGGCCACG ACTGGGACTC ACCGGCATAT
    CGTTTCATCC GCTTTGACCA CATCCCAGCT GTGAGCACCC CGGCGCTCCA TCAGATCCTT
    AAACAGGTCC AGAACAATGA GGGCTTTGTG TTTGTGGCCA CCATGAGACA AGACAAGGGC
    TCCAGGGGCA CCTTACTTGG AGTGGAGGGT CCCGGAGGGC TCCGTCAGCT TGAGATAGTG
    TCCAATGGTC GCGCCAACAC ACTGGATCTG GTCTACATGG TAAAGGGATC CCAGAACGTG
    GTGTCCTTCG AGGATGTGGA CCTGTCTGAT TCGCAGTGGA AGAACATCTC GCTGTACGTT
    CATGGGGAAA ACGCTCACCT GTATGTGGGC TGCAGCCTCA TAGACAGCGT GATCCTGGAT
    GAGCCTTTCT ATGAGCTCCT GCGTCCAGAG GGCAGCCAGA TGTTTGTGGC CAAGGGATCT
    ATCAGGGAAA ACCACTTCAG GGGCTTACTG CAAAATGTTC AGTTCCTTTT TGACACTCCA
    ATAGAAAACA TCCTGAGAAA CAAAGGCTGT GAGATTGCCA AACCTGAGGA AGTTAACGTG
    GTTAATGAGA GTACAGAATC TGTGTCTGTC GGTACATCTA TATCTACCAA CTTCATTGGG
    GAGAAGGAGA AAATAGCATC AGACGTGTGC GAGCGCTCTT GTGAAGAGCT CAGTAACATG
    GTCCAGGAGC TCAAAGGTCT TCGAATCATC GTGGGCAATC TCATCGATGG CTTGCAGAAA
    GTGACTGAGG AGAACACCGT GATGAAGGAG GTGCTGGGAA ACATGAAGAA CATCAAAGAC
    AAGCGCATGT GCTGGCAAGA CGGCCGTCTC TTTGAGGATA AAGAGGACTG GGTTGTGGAC
    AGCTGCACCA AGTGTACCTG TCAGGAATCA AAGATTGTGT GCCATCAAAT CACATGTCCA
    CCAGTAGCAT GTGCCAGCCC CACTTTCCTA GATGGCGAGT GCTGTCCACT GTGTCTGCCT
    AAAGACAGCG AGGACGGCTG GTCACCTTGG TCTGAATGGA CAGAGTGTAC GGTCACATGT
    GGGACGGGCA CGCAACAAAG AGGCAGGTCA TGTGATGCCA CAAGTAACCC CTGCAGTGGC
    CCATCTATCC AAACCCGCAG ATGCAGCTTG GGAAAATGCG ACAGTCGTGT TCGTCAAGAT
    GGTGGTTGGA GCTTGTGGTC CCCCTGGTCA TCTTGCTCTG TGACGTGTGG AGAAGGACAA
    ATCACACGAA TTCGCCACTG CAACTCTCCT GTTCCTCAGC TGGGAGGAAA GGACTGTGAG
    GGCAGTGGGA GAGAGACGCA AAAGTGTGAA GCTAAACCCT GTCCAATTGA TGGAGCCTGG
    GGCCCCTGGT CTCCTTGGGC AGTCTGCTCT GTCACCTGTG GTGGAGGAAC GAGGACACGA
    ACACGTGTGT GTAACAGCCC TCGTCCACAG TATGGAGGAA AGAAATGCCC TGGAGATTCC
    GTAGACACTA ATGCCTGTAA TAAACAGGAC TGTCCTGTGG ATGGTTGTCT GTCTAACCCA
    TGTTTCGGGG GTGTTGAATG CACAAGCTCT CCTGACGGCT CGTGGGAGTG TGGTCCGTGC
    CCTCTCGGCT TCCGGGGCAA TGGAAAAGTG TGTGAGGATG TCAATGAGTG TGACATGGTC
    CCCGATCTGT GCTATAAAGT GGGTGATTCC CAGCGTTGTG TCAACACAGA CCCAGGATTC
    CACTGCTTGC CTTGTCCTCC TCGATACAAA GGCAATCAGC CGTTTGGAAT GGGAGTGGAG
    GCTGCCAAGC TAAATAAACA GGTGTGTGAG CAAGAAAACC CCTGCAAGGA GAAAACCCAC
    AACTGCCACA GGTCTGCTGA GTGCATTTAC CTTGGCCACT TCAGCGAACC CATGTTTAAG
    TGTGAGTGTA AGATCGGTTA TGCTGGGGAT GGACTCATCT GTGGCGAGGA CTCAGACCTT
    GATGGCTGGC CTAATCAGAA CCTGGTGTGT GGAGCCAACC AATCCTACCA CTGCAAAAAG
    GACAATTGTC CCAAACTTCC AAACTCAGGC CAAGAGGATT TTGATAAAGA CGGTCAAGGT
    GACGCCTGCG ACAAAGACGA TGATAATGAT GGAATCATGG ATGAGACTGA CAACTGTCCT
    CTACTGTATA ATCCCAGGCA ATTTGACTAT GATAAAGATC TTGTCGGTGA CCGCTGTGAT
    AACTGCCCAT ACGAGCACAA TCCTGCTCAG ATTGACACAG ACGGTAATGG CGAAGGGGAC
    GCCTGTGCTG TTGACATTGA TGGAGATGAG GTTCTGAATG AGAATGATAA CTGTCCCTAC
    ATGTACAACA CTGACCAGAA GGACACGGAT ATGGATGGAG TGGGTGACCA GTGTGACAAC
    TGCCCTCTGC TGCACAACCC TGAGCAGACT GACTCAGATA ATGACCGAGT TGGTGACGAG
    TGCGACAACA ATCAAGACAT TGATGAGGAC GGCCACCAGA ACAGCCTGGA TAATTGTCCT
    TACATTCCCA ACGCCAACCA GGCCGACCAC GACAAAGATG GGAAAGGAGA CGCTTGCGAT
    TCCGATGATG ATAATGACGG AGTCCCAGAT GATAAGGACA ACTGCAGACT GGTGCCCAAC
    AAAGACCAAC GAGACTCTGA TGGGGATGGA AGAGGCGACG CCTGCAAAGA CGATTTTGAC
    AACGACAGCA TTCCAGACTT TATGGACGTG TGTCCTGAGA ACGATGCCAT CAGCAACACA
    GACTTCCGCA AGTTTCAGAT GGTGCATTTG GATCCCAAAG GAACTGCGCA AATTGATCCC
    AACTGGGTGG TCAGACATCA AGGCAAAGAG CTCGTTCAGA CCGCCAACTC GGACCCTGGC
    ATTGCAGTGG GCTTCGACGA ATTCAACGCT GTGGATTTCA GCGGCACTTT TTACGTCAAC
    ACAGATAGAG ATGATGATTA TGCTGGCTTT GTGTTCGGCT ATCAGTCCAG CGGGCGCTTT
    TATGTGGTAA TGTGGAAGCA GATAACGCAG ACATACTGGG AGGACAATCC TTCAAAAGCC
    ATCGGCATCT CTGGGGTTTC TCTGAAAGTG GTCAACTCGA CCACAGGCAC AGGAGAGCAC
    CTTCGCAATG CCCTGTGGCA CACTGGGGAC ACACCTGGAC AGGTTCGCAC ACTGTGGCAC
    GACCCTAAGA ACATCGGCTG GAAGGATTAC ACCGCCTACA GGTGGCACCT CATTCACAGA
    CCCAAGACTG GATTCATCAG AGTGGTGGTT TATGAGGGGA AACAGATCAT GGCAGACTCA
    GGACCAGTTT ATGATCAAAC ATTTGCAGGA GGGAGATTAG GACTCTTCGT CTTCTCTCAG
    GAACTGGTTT ATTTCTCTGA TCTCAAGTAT GAATGTCGAG ATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305765.1
    CDS733..4260
    Translation

    Target ORF information:

    RefSeq Version XM_009307490.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio thrombospondin 2a (thbs2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATACTCA GGAGTAACCT CTGCTTGTGG CCTTTGCTGT TACTATGTAT CAGAGCACTA 
    CCTCAAGATG GCCTTGTGGA AGATGAATCA ATCTTCGATC TCTTCAAAAT CAGCGGCATT
    AGCAGAAAAA CGATTGGAGC CAAGCTCTTC AAGGGCCACG ACTGGGACTC ACCGGCATAT
    CGTTTCATCC GCTTTGACCA CATCCCAGCT GTGAGCACCC CGGCGCTCCA TCAGATCCTT
    AAACAGGTCC AGAACAATGA GGGCTTTGTG TTTGTGGCCA CCATGAGACA AGACAAGGGC
    TCCAGGGGCA CCTTACTTGG AGTGGAGGGT CCCGGAGGGC TCCGTCAGCT TGAGATAGTG
    TCCAATGGTC GCGCCAACAC ACTGGATCTG GTCTACATGG TAAAGGGATC CCAGAACGTG
    GTGTCCTTCG AGGATGTGGA CCTGTCTGAT TCGCAGTGGA AGAACATCTC GCTGTACGTT
    CATGGGGAAA ACGCTCACCT GTATGTGGGC TGCAGCCTCA TAGACAGCGT GATCCTGGAT
    GAGCCTTTCT ATGAGCTCCT GCGTCCAGAG GGCAGCCAGA TGTTTGTGGC CAAGGGATCT
    ATCAGGGAAA ACCACTTCAG GGGCTTACTG CAAAATGTTC AGTTCCTTTT TGACACTCCA
    ATAGAAAACA TCCTGAGAAA CAAAGGCTGT GAGATTGCCA AACCTGAGGA AGTTAACGTG
    GTTAATGAGA GTACAGAATC TGTGTCTGTC GGTACATCTA TATCTACCAA CTTCATTGGG
    GAGAAGGAGA AAATAGCATC AGACGTGTGC GAGCGCTCTT GTGAAGAGCT CAGTAACATG
    GTCCAGGAGC TCAAAGGTCT TCGAATCATC GTGGGCAATC TCATCGATGG CTTGCAGAAA
    GTGACTGAGG AGAACACCGT GATGAAGGAG GTGCTGGGAA ACATGAAGAA CATCAAAGAC
    AAGCGCATGT GCTGGCAAGA CGGCCGTCTC TTTGAGGATA AAGAGGACTG GGTTGTGGAC
    AGCTGCACCA AGTGTACCTG TCAGGAATCA AAGATTGTGT GCCATCAAAT CACATGTCCA
    CCAGTAGCAT GTGCCAGCCC CACTTTCCTA GATGGCGAGT GCTGTCCACT GTGTCTGCCT
    AAAGACAGCG AGGACGGCTG GTCACCTTGG TCTGAATGGA CAGAGTGTAC GGTCACATGT
    GGGACGGGCA CGCAACAAAG AGGCAGGTCA TGTGATGCCA CAAGTAACCC CTGCAGTGGC
    CCATCTATCC AAACCCGCAG ATGCAGCTTG GGAAAATGCG ACAGTCGTGT TCGTCAAGAT
    GGTGGTTGGA GCTTGTGGTC CCCCTGGTCA TCTTGCTCTG TGACGTGTGG AGAAGGACAA
    ATCACACGAA TTCGCCACTG CAACTCTCCT GTTCCTCAGC TGGGAGGAAA GGACTGTGAG
    GGCAGTGGGA GAGAGACGCA AAAGTGTGAA GCTAAACCCT GTCCAATTGA TGGAGCCTGG
    GGCCCCTGGT CTCCTTGGGC AGTCTGCTCT GTCACCTGTG GTGGAGGAAC GAGGACACGA
    ACACGTGTGT GTAACAGCCC TCGTCCACAG TATGGAGGAA AGAAATGCCC TGGAGATTCC
    GTAGACACTA ATGCCTGTAA TAAACAGGAC TGTCCTGTGG ATGGTTGTCT GTCTAACCCA
    TGTTTCGGGG GTGTTGAATG CACAAGCTCT CCTGACGGCT CGTGGGAGTG TGGTCCGTGC
    CCTCTCGGCT TCCGGGGCAA TGGAAAAGTG TGTGAGGATG TCAATGAGTG TGACATGGTC
    CCCGATCTGT GCTATAAAGT GGGTGATTCC CAGCGTTGTG TCAACACAGA CCCAGGATTC
    CACTGCTTGC CTTGTCCTCC TCGATACAAA GGCAATCAGC CGTTTGGAAT GGGAGTGGAG
    GCTGCCAAGC TAAATAAACA GGTGTGTGAG CAAGAAAACC CCTGCAAGGA GAAAACCCAC
    AACTGCCACA GGTCTGCTGA GTGCATTTAC CTTGGCCACT TCAGCGAACC CATGTTTAAG
    TGTGAGTGTA AGATCGGTTA TGCTGGGGAT GGACTCATCT GTGGCGAGGA CTCAGACCTT
    GATGGCTGGC CTAATCAGAA CCTGGTGTGT GGAGCCAACC AATCCTACCA CTGCAAAAAG
    GACAATTGTC CCAAACTTCC AAACTCAGGC CAAGAGGATT TTGATAAAGA CGGTCAAGGT
    GACGCCTGCG ACAAAGACGA TGATAATGAT GGAATCATGG ATGAGACTGA CAACTGTCCT
    CTACTGTATA ATCCCAGGCA ATTTGACTAT GATAAAGATC TTGTCGGTGA CCGCTGTGAT
    AACTGCCCAT ACGAGCACAA TCCTGCTCAG ATTGACACAG ACGGTAATGG CGAAGGGGAC
    GCCTGTGCTG TTGACATTGA TGGAGATGAG GTTCTGAATG AGAATGATAA CTGTCCCTAC
    ATGTACAACA CTGACCAGAA GGACACGGAT ATGGATGGAG TGGGTGACCA GTGTGACAAC
    TGCCCTCTGC TGCACAACCC TGAGCAGACT GACTCAGATA ATGACCGAGT TGGTGACGAG
    TGCGACAACA ATCAAGACAT TGATGAGGAC GGCCACCAGA ACAGCCTGGA TAATTGTCCT
    TACATTCCCA ACGCCAACCA GGCCGACCAC GACAAAGATG GGAAAGGAGA CGCTTGCGAT
    TCCGATGATG ATAATGACGG AGTCCCAGAT GATAAGGACA ACTGCAGACT GGTGCCCAAC
    AAAGACCAAC GAGACTCTGA TGGGGATGGA AGAGGCGACG CCTGCAAAGA CGATTTTGAC
    AACGACAGCA TTCCAGACTT TATGGACGTG TGTCCTGAGA ACGATGCCAT CAGCAACACA
    GACTTCCGCA AGTTTCAGAT GGTGCATTTG GATCCCAAAG GAACTGCGCA AATTGATCCC
    AACTGGGTGG TCAGACATCA AGGCAAAGAG CTCGTTCAGA CCGCCAACTC GGACCCTGGC
    ATTGCAGTGG GCTTCGACGA ATTCAACGCT GTGGATTTCA GCGGCACTTT TTACGTCAAC
    ACAGATAGAG ATGATGATTA TGCTGGCTTT GTGTTCGGCT ATCAGTCCAG CGGGCGCTTT
    TATGTGGTAA TGTGGAAGCA GATAACGCAG ACATACTGGG AGGACAATCC TTCAAAAGCC
    ATCGGCATCT CTGGGGTTTC TCTGAAAGTG GTCAACTCGA CCACAGGCAC AGGAGAGCAC
    CTTCGCAATG CCCTGTGGCA CACTGGGGAC ACACCTGGAC AGGTTCGCAC ACTGTGGCAC
    GACCCTAAGA ACATCGGCTG GAAGGATTAC ACCGCCTACA GGTGGCACCT CATTCACAGA
    CCCAAGACTG GATTCATCAG AGTGGTGGTT TATGAGGGGA AACAGATCAT GGCAGACTCA
    GGACCAGTTT ATGATCAAAC ATTTGCAGGA GGGAGATTAG GACTCTTCGT CTTCTCTCAG
    GAACTGGTTT ATTTCTCTGA TCTCAAGTAT GAATGTCGAG ATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.