nlrc5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nlrc5
Entrez Gene ID	100535184
Full Name	NLR family, CARD domain containing 5
Synonyms	nod4,si:ch211-122c9.5
General protein information	Preferred Names NLR family, CARD domain containing 5 Names protein NLRC5 NLRC5a protein NLRC5b protein NLRC5c protein NLRC5d protein NLRC5e protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017352039.1	XP_017207528.1	protein NLRC5 isoform X1
XM_017352040.2	XP_017207529.1	protein NLRC5 isoform X2
XM_017352040.1	XP_017207529.1	protein NLRC5 isoform X2
XM_003200446.4	XP_003200494.2	protein NLRC5 isoform X1
XM_003200446.5	XP_003200494.2	protein NLRC5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NLRC5	NP_115582.4
Gallus gallus (chicken)	NLRC5	XP_004944113.1
Bos taurus (cattle)	NLRC5	XP_002694836.1
Danio rerio (zebrafish)	si:ch211-122c9.5	XP_003200494.2
Xenopus tropicalis (tropical clawed frog)	LOC101730474	XP_004914291.1
Pan troglodytes (chimpanzee)	NLRC5	XP_003952898.1
	NLRC5	XP_001095341.2
Mus musculus (house mouse)	Nlrc5	NP_001028379.2
Canis lupus familiaris (dog)	NLRC5	XP_544394.4

Gene Ontology

The following nlrc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nlrc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55896	XM_017352040.2 Latest version!	Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa23318	XM_003200446.5 Latest version!	Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23318	XM_017352039.1 Latest version!	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa55896	XM_017352040.1	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa23318	XM_003200446.4	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nlrc5 ( XM_017352040.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207529.1 Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X2, mRNA.

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CloneID	ODa55896
Clone ID Related Accession (Same CDS sequence)	XM_017352040.2 , XM_017352040.1
Accession Version	XM_017352040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTAAGTC ATGTTGATCT GTCTACAAAT 
GGGGCTTTGA TATTGGTCAG ATCTCTTGCC GATTGTCAGC GTGTCACTTC TGTCGAACTC 
AGGCACCAAG CTGAAGCATT CATCAAATTT GTTCCTGAGA AAGCTGAAGT AGCAATTTGC 
AAGCTCACAG AGTATAATCT GAGCAAAACC AACGTCGTGA AGCTCTCGAA GATACTGGAG 
AGCTGTCCTC ATCTGTCTGA ACTGGACTTG TCTCATAACG TTCTCAGAGA TGAAGGTGTG 
AAGTGTTTTG TTGACCATTT GCCCAAACTA AAGATCTCCA GCTCAGTGAG CCTCATTGGC 
AACCGAATGA CTCAAATCGG GGCACTGCAT CTGGTCAACT CAATGAATAC CTGTGAAAAA 
GTTGTTGCTG TCGAAGTCAG TCTTGGAGTG GAGGATCAGT CTCTGATTCA GTTTGTGCAA 
GAACATGCCA ATGGCAAGAC TCTTAGGCTA AGGGAGTGCA GTTTTGAGCT TGCACACTTG 
AAGATGCTTG TGAAAATCCT GACAAAATGC CCAAGACTCC TTTCATTAGA ATTGTCTTCT 
AATTCACTGA ACAGTCAAGG ACTTTTTTCC TTACTGGATA GCCTGGCTGA ACTATCCACA 
ATCCAAACTT TAACGTTGAG AAATAATGGC CTGAATTCTG AGCAAATTGA GCATTTTGTT 
AAACAGTTTA GTCACTGCAA CCAACACAGA GACATCAGAA TCGAAGAGTC GTGGATGACA 
GGAAGTGCTG CTATTGGCCT CATTGCGAGA TGCCTCAGTC TACAGCAGCA CATCAGAGAA 
ATCAGGGTCC ACCGTGCATC TATAAACATG ATCATTGAAG CCAATTCCAT TTCCAGCTCC 
ACTTCAGTCG GTCATTCTGA AAATGTGTTG CCTGCTCTGC AGTCTATCAG CTTTGATGAC 
TGTGATGTAG AAGGACAGCA CCTCACTCAA CTCGCACTTC CCATCCAGAA ATGTCATGCC 
TTACTGGATT TAAAGTTCTC GCCACTTAAA TTGGACAGAA TAGGTGCAGA GTTTTTTTCT 
GCAGTCATTC CATTCTTAAG GAATCTCAGG AACATCACTC TGGATCTGAA AGGAGCCGCT 
GAGGAGGAAG TGGAAACTCT TGCTGAGGCC CTGCCAAACA CTCCAGCCTT TGAGAGTCTG 
AGCTTGTCCC AGCTTGTCTT AAGTGACCGT GGAGCTACAC TATTCGGAAA AGCTTTTCAA 
AATGTGCCAA ATCTGAAGTC ATTCAGTATG TTGCAGTGCT CTGGTTGGAC AGCAGCTCGA 
GGCTGTGACT TGGTCAGAGG CTTGATGCAG TGTGTTTTGC TGACAGAAAT ACGACTGGAG 
TCTGTGGTAC TCGACCAGGA GGGTATAAAT ATCCTAGCTC AAGGCTTGAG TCGACTGGCC 
TCACTTCGGA GGCTCAGCTT AAACAACATT ACAGTGGTGA CATCTGAGAC TTTTGTAAAT 
GGCACTGGAA TACTCTGTCT ACTGGCCTCT TTTAAAGGTT TCAAACAGAT GGAGGAGATT 
GAGTTGGAAA GTTGGCGGAT GGGAGATGGT GGTACCAATG AGCTTGTGAA ATACATACCT 
TCATGGACAG GGCTGGTAAA AATAAGTCTG TCAGAAAACA TTGTGACTGA CCAGGCCGGA 
GAGAAACTCC TCACAGCACT TTCCCATTGC AGAGCTCTTC AGCAGCTCAA GTTGTCTAAA 
AACAAGCTTG GTCAGGCCAG TGCTGCCAAG CTTGCTAAAA TTCTTCCATT GCTACCTCAG 
CTTTCAGAAC TCAATTTATC AGAGAATCAG TTTGGCCCAC AAGGATCAAG TGTTCTTTGT 
GAAGGTTTGG TGAGCATGAA GGCACTGAAA AAACTACATT TGACTTCGGT TGAGTCTTCA 
GATCTGGTTG GTGTGGCTTC ATCCTTAAAA CACTGTCCAT CCATAGAGGA TGTCAGTTTG 
TCATGGAATG GATGTGGTGA CAGTCTTGCA CGGACACTGG CTGAGATTCT TCCCTTGTGT 
ACGAAGCTGA AAAGACTCGA CCTGGAGGCT AATAACATAA CCACAGCGGG AGCCACACTG 
ATTGCCAAAT GCCTCCCATT ATGCCCATCC ATTGAAGTTA TAAGACTCTG GAGGAACTCA 
ATTGCCAAAG ATGATGAAAA TCTGAAAGAT CCCAAACTCA ACTTTTCATC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207529.1
CDS	508..5664
Translation	MRMERMDWSGDALTLVAQEASVLVDLLCEQDSSVLDHIFDLLESNTQDEIRSLINNRDRVSAIVDYFKTSDNITCRKFFCTIYEYCKDIPFYLETTLVSIAGDPIDNYSSSRNVKRLRLDPLQSYTDALKSMVKEKHKTVMQSVVKDIRLDDTWVYLKHRNPPRAKDRTLQLQASLDSHEGEESEHKVSVESLLKTTGRVVVLLGQAGSGKTLLVHCLGHSWAENTFPSIQLLFLLEFRQLNLISRDLSLKELLFLFYPACSYVEEEKEEVFAFILTHPDKVCFILDGYDEFRAKLTDPRELESTVDLSTPMPMADLLSGLCSRKILPDCTVLVTCRPRDVTDMFGSPGLITCELQGFDRLGVKEYAEQYFHEKGDLKTNAVNLLMDNRHLLSMSHVPGLCHICCICVDHLFSSGEVLNHQLPTSLTQIYIQILLAFLSRFNEDGVSKTSLLMRYRTKIVEMSQLALKGLEGSTIVFMETEVSKELQDFGVRSGILSRVKLTHEDGSSGYGFTFMHLTMQEFLAALHLMTSQTITDGQLKKKLNLKTRWTTKTDPKSVFTDSVHLYVCGLAAEACTSCLVQLKGAESETTWVKKRQAAVRNVLLGLAGGASHTGPKIVELCRCAHETQDAKLAKAIGSRPQIELRNIRLNAVDMDSLAFVTAAADQTVCLDFGGCSIDLDCLEILPSFKNVDHLIFRSRKYDDKFAETLCGILSKLQALQQLDFISGGLTDVGAAKLAKALEDCPNITHLNVSDNSLKDEGIREIAETVSRLHNISSILMGKNNISTDGILTLIERMAACTSVQEVHVEGNKEINLSFSKRSDTISESKDLKNTEETKNIILKQCNLTVSHVASLCNKLRGFAILTLLDLSNNSLGNKGLKKILDLLPKLGGIQEINVSENGVDMEGVVMLASALCKQYNLKRVDASENGKKKVVLGFDTTRGHNSGELTQNETGMQLHKKLSLTHSDIQPAYMTKLCKQLVKCKNLLDIDLSHVDLSTNGALILVRSLADCQRVTSVELRHQAEAFIKFVPEKAEVAICKLTEYNLSKTNVVKLSKILESCPHLSELDLSHNVLRDEGVKCFVDHLPKLKISSSVSLIGNRMTQIGALHLVNSMNTCEKVVAVEVSLGVEDQSLIQFVQEHANGKTLRLRECSFELAHLKMLVKILTKCPRLLSLELSSNSLNSQGLFSLLDSLAELSTIQTLTLRNNGLNSEQIEHFVKQFSHCNQHRDIRIEESWMTGSAAIGLIARCLSLQQHIREIRVHRASINMIIEANSISSSTSVGHSENVLPALQSISFDDCDVEGQHLTQLALPIQKCHALLDLKFSPLKLDRIGAEFFSAVIPFLRNLRNITLDLKGAAEEEVETLAEALPNTPAFESLSLSQLVLSDRGATLFGKAFQNVPNLKSFSMLQCSGWTAARGCDLVRGLMQCVLLTEIRLESVVLDQEGINILAQGLSRLASLRRLSLNNITVVTSETFVNGTGILCLLASFKGFKQMEEIELESWRMGDGGTNELVKYIPSWTGLVKISLSENIVTDQAGEKLLTALSHCRALQQLKLSKNKLGQASAAKLAKILPLLPQLSELNLSENQFGPQGSSVLCEGLVSMKALKKLHLTSVESSDLVGVASSLKHCPSIEDVSLSWNGCGDSLARTLAEILPLCTKLKRLDLEANNITTAGATLIAKCLPLCPSIEVIRLWRNSIAKDDENLKDPKLNFSSV

Target ORF information:

RefSeq Version	XM_017352040.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352040.2

ORF Insert Sequence:

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTAAGTC ATGTTGATCT GTCTACAAAT 
GGGGCTTTGA TATTGGTCAG ATCTCTTGCC GATTGTCAGC GTGTCACTTC TGTCGAACTC 
AGGCACCAAG CTGAAGCATT CATCAAATTT GTTCCTGAGA AAGCTGAAGT AGCAATTTGC 
AAGCTCACAG AGTATAATCT GAGCAAAACC AACGTCGTGA AGCTCTCGAA GATACTGGAG 
AGCTGTCCTC ATCTGTCTGA ACTGGACTTG TCTCATAACG TTCTCAGAGA TGAAGGTGTG 
AAGTGTTTTG TTGACCATTT GCCCAAACTA AAGATCTCCA GCTCAGTGAG CCTCATTGGC 
AACCGAATGA CTCAAATCGG GGCACTGCAT CTGGTCAACT CAATGAATAC CTGTGAAAAA 
GTTGTTGCTG TCGAAGTCAG TCTTGGAGTG GAGGATCAGT CTCTGATTCA GTTTGTGCAA 
GAACATGCCA ATGGCAAGAC TCTTAGGCTA AGGGAGTGCA GTTTTGAGCT TGCACACTTG 
AAGATGCTTG TGAAAATCCT GACAAAATGC CCAAGACTCC TTTCATTAGA ATTGTCTTCT 
AATTCACTGA ACAGTCAAGG ACTTTTTTCC TTACTGGATA GCCTGGCTGA ACTATCCACA 
ATCCAAACTT TAACGTTGAG AAATAATGGC CTGAATTCTG AGCAAATTGA GCATTTTGTT 
AAACAGTTTA GTCACTGCAA CCAACACAGA GACATCAGAA TCGAAGAGTC GTGGATGACA 
GGAAGTGCTG CTATTGGCCT CATTGCGAGA TGCCTCAGTC TACAGCAGCA CATCAGAGAA 
ATCAGGGTCC ACCGTGCATC TATAAACATG ATCATTGAAG CCAATTCCAT TTCCAGCTCC 
ACTTCAGTCG GTCATTCTGA AAATGTGTTG CCTGCTCTGC AGTCTATCAG CTTTGATGAC 
TGTGATGTAG AAGGACAGCA CCTCACTCAA CTCGCACTTC CCATCCAGAA ATGTCATGCC 
TTACTGGATT TAAAGTTCTC GCCACTTAAA TTGGACAGAA TAGGTGCAGA GTTTTTTTCT 
GCAGTCATTC CATTCTTAAG GAATCTCAGG AACATCACTC TGGATCTGAA AGGAGCCGCT 
GAGGAGGAAG TGGAAACTCT TGCTGAGGCC CTGCCAAACA CTCCAGCCTT TGAGAGTCTG 
AGCTTGTCCC AGCTTGTCTT AAGTGACCGT GGAGCTACAC TATTCGGAAA AGCTTTTCAA 
AATGTGCCAA ATCTGAAGTC ATTCAGTATG TTGCAGTGCT CTGGTTGGAC AGCAGCTCGA 
GGCTGTGACT TGGTCAGAGG CTTGATGCAG TGTGTTTTGC TGACAGAAAT ACGACTGGAG 
TCTGTGGTAC TCGACCAGGA GGGTATAAAT ATCCTAGCTC AAGGCTTGAG TCGACTGGCC 
TCACTTCGGA GGCTCAGCTT AAACAACATT ACAGTGGTGA CATCTGAGAC TTTTGTAAAT 
GGCACTGGAA TACTCTGTCT ACTGGCCTCT TTTAAAGGTT TCAAACAGAT GGAGGAGATT 
GAGTTGGAAA GTTGGCGGAT GGGAGATGGT GGTACCAATG AGCTTGTGAA ATACATACCT 
TCATGGACAG GGCTGGTAAA AATAAGTCTG TCAGAAAACA TTGTGACTGA CCAGGCCGGA 
GAGAAACTCC TCACAGCACT TTCCCATTGC AGAGCTCTTC AGCAGCTCAA GTTGTCTAAA 
AACAAGCTTG GTCAGGCCAG TGCTGCCAAG CTTGCTAAAA TTCTTCCATT GCTACCTCAG 
CTTTCAGAAC TCAATTTATC AGAGAATCAG TTTGGCCCAC AAGGATCAAG TGTTCTTTGT 
GAAGGTTTGG TGAGCATGAA GGCACTGAAA AAACTACATT TGACTTCGGT TGAGTCTTCA 
GATCTGGTTG GTGTGGCTTC ATCCTTAAAA CACTGTCCAT CCATAGAGGA TGTCAGTTTG 
TCATGGAATG GATGTGGTGA CAGTCTTGCA CGGACACTGG CTGAGATTCT TCCCTTGTGT 
ACGAAGCTGA AAAGACTCGA CCTGGAGGCT AATAACATAA CCACAGCGGG AGCCACACTG 
ATTGCCAAAT GCCTCCCATT ATGCCCATCC ATTGAAGTTA TAAGACTCTG GAGGAACTCA 
ATTGCCAAAG ATGATGAAAA TCTGAAAGAT CCCAAACTCA ACTTTTCATC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlrc5 ( XM_017352040.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207529.1 Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X3, mRNA.

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CloneID	ODa55896
Clone ID Related Accession (Same CDS sequence)	XM_017352040.2 , XM_017352040.1
Accession Version	XM_017352040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTAAGTC ATGTTGATCT GTCTACAAAT 
GGGGCTTTGA TATTGGTCAG ATCTCTTGCC GATTGTCAGC GTGTCACTTC TGTCGAACTC 
AGGCACCAAG CTGAAGCATT CATCAAATTT GTTCCTGAGA AAGCTGAAGT AGCAATTTGC 
AAGCTCACAG AGTATAATCT GAGCAAAACC AACGTCGTGA AGCTCTCGAA GATACTGGAG 
AGCTGTCCTC ATCTGTCTGA ACTGGACTTG TCTCATAACG TTCTCAGAGA TGAAGGTGTG 
AAGTGTTTTG TTGACCATTT GCCCAAACTA AAGATCTCCA GCTCAGTGAG CCTCATTGGC 
AACCGAATGA CTCAAATCGG GGCACTGCAT CTGGTCAACT CAATGAATAC CTGTGAAAAA 
GTTGTTGCTG TCGAAGTCAG TCTTGGAGTG GAGGATCAGT CTCTGATTCA GTTTGTGCAA 
GAACATGCCA ATGGCAAGAC TCTTAGGCTA AGGGAGTGCA GTTTTGAGCT TGCACACTTG 
AAGATGCTTG TGAAAATCCT GACAAAATGC CCAAGACTCC TTTCATTAGA ATTGTCTTCT 
AATTCACTGA ACAGTCAAGG ACTTTTTTCC TTACTGGATA GCCTGGCTGA ACTATCCACA 
ATCCAAACTT TAACGTTGAG AAATAATGGC CTGAATTCTG AGCAAATTGA GCATTTTGTT 
AAACAGTTTA GTCACTGCAA CCAACACAGA GACATCAGAA TCGAAGAGTC GTGGATGACA 
GGAAGTGCTG CTATTGGCCT CATTGCGAGA TGCCTCAGTC TACAGCAGCA CATCAGAGAA 
ATCAGGGTCC ACCGTGCATC TATAAACATG ATCATTGAAG CCAATTCCAT TTCCAGCTCC 
ACTTCAGTCG GTCATTCTGA AAATGTGTTG CCTGCTCTGC AGTCTATCAG CTTTGATGAC 
TGTGATGTAG AAGGACAGCA CCTCACTCAA CTCGCACTTC CCATCCAGAA ATGTCATGCC 
TTACTGGATT TAAAGTTCTC GCCACTTAAA TTGGACAGAA TAGGTGCAGA GTTTTTTTCT 
GCAGTCATTC CATTCTTAAG GAATCTCAGG AACATCACTC TGGATCTGAA AGGAGCCGCT 
GAGGAGGAAG TGGAAACTCT TGCTGAGGCC CTGCCAAACA CTCCAGCCTT TGAGAGTCTG 
AGCTTGTCCC AGCTTGTCTT AAGTGACCGT GGAGCTACAC TATTCGGAAA AGCTTTTCAA 
AATGTGCCAA ATCTGAAGTC ATTCAGTATG TTGCAGTGCT CTGGTTGGAC AGCAGCTCGA 
GGCTGTGACT TGGTCAGAGG CTTGATGCAG TGTGTTTTGC TGACAGAAAT ACGACTGGAG 
TCTGTGGTAC TCGACCAGGA GGGTATAAAT ATCCTAGCTC AAGGCTTGAG TCGACTGGCC 
TCACTTCGGA GGCTCAGCTT AAACAACATT ACAGTGGTGA CATCTGAGAC TTTTGTAAAT 
GGCACTGGAA TACTCTGTCT ACTGGCCTCT TTTAAAGGTT TCAAACAGAT GGAGGAGATT 
GAGTTGGAAA GTTGGCGGAT GGGAGATGGT GGTACCAATG AGCTTGTGAA ATACATACCT 
TCATGGACAG GGCTGGTAAA AATAAGTCTG TCAGAAAACA TTGTGACTGA CCAGGCCGGA 
GAGAAACTCC TCACAGCACT TTCCCATTGC AGAGCTCTTC AGCAGCTCAA GTTGTCTAAA 
AACAAGCTTG GTCAGGCCAG TGCTGCCAAG CTTGCTAAAA TTCTTCCATT GCTACCTCAG 
CTTTCAGAAC TCAATTTATC AGAGAATCAG TTTGGCCCAC AAGGATCAAG TGTTCTTTGT 
GAAGGTTTGG TGAGCATGAA GGCACTGAAA AAACTACATT TGACTTCGGT TGAGTCTTCA 
GATCTGGTTG GTGTGGCTTC ATCCTTAAAA CACTGTCCAT CCATAGAGGA TGTCAGTTTG 
TCATGGAATG GATGTGGTGA CAGTCTTGCA CGGACACTGG CTGAGATTCT TCCCTTGTGT 
ACGAAGCTGA AAAGACTCGA CCTGGAGGCT AATAACATAA CCACAGCGGG AGCCACACTG 
ATTGCCAAAT GCCTCCCATT ATGCCCATCC ATTGAAGTTA TAAGACTCTG GAGGAACTCA 
ATTGCCAAAG ATGATGAAAA TCTGAAAGAT CCCAAACTCA ACTTTTCATC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207529.1
CDS	504..5660
Translation	MRMERMDWSGDALTLVAQEASVLVDLLCEQDSSVLDHIFDLLESNTQDEIRSLINNRDRVSAIVDYFKTSDNITCRKFFCTIYEYCKDIPFYLETTLVSIAGDPIDNYSSSRNVKRLRLDPLQSYTDALKSMVKEKHKTVMQSVVKDIRLDDTWVYLKHRNPPRAKDRTLQLQASLDSHEGEESEHKVSVESLLKTTGRVVVLLGQAGSGKTLLVHCLGHSWAENTFPSIQLLFLLEFRQLNLISRDLSLKELLFLFYPACSYVEEEKEEVFAFILTHPDKVCFILDGYDEFRAKLTDPRELESTVDLSTPMPMADLLSGLCSRKILPDCTVLVTCRPRDVTDMFGSPGLITCELQGFDRLGVKEYAEQYFHEKGDLKTNAVNLLMDNRHLLSMSHVPGLCHICCICVDHLFSSGEVLNHQLPTSLTQIYIQILLAFLSRFNEDGVSKTSLLMRYRTKIVEMSQLALKGLEGSTIVFMETEVSKELQDFGVRSGILSRVKLTHEDGSSGYGFTFMHLTMQEFLAALHLMTSQTITDGQLKKKLNLKTRWTTKTDPKSVFTDSVHLYVCGLAAEACTSCLVQLKGAESETTWVKKRQAAVRNVLLGLAGGASHTGPKIVELCRCAHETQDAKLAKAIGSRPQIELRNIRLNAVDMDSLAFVTAAADQTVCLDFGGCSIDLDCLEILPSFKNVDHLIFRSRKYDDKFAETLCGILSKLQALQQLDFISGGLTDVGAAKLAKALEDCPNITHLNVSDNSLKDEGIREIAETVSRLHNISSILMGKNNISTDGILTLIERMAACTSVQEVHVEGNKEINLSFSKRSDTISESKDLKNTEETKNIILKQCNLTVSHVASLCNKLRGFAILTLLDLSNNSLGNKGLKKILDLLPKLGGIQEINVSENGVDMEGVVMLASALCKQYNLKRVDASENGKKKVVLGFDTTRGHNSGELTQNETGMQLHKKLSLTHSDIQPAYMTKLCKQLVKCKNLLDIDLSHVDLSTNGALILVRSLADCQRVTSVELRHQAEAFIKFVPEKAEVAICKLTEYNLSKTNVVKLSKILESCPHLSELDLSHNVLRDEGVKCFVDHLPKLKISSSVSLIGNRMTQIGALHLVNSMNTCEKVVAVEVSLGVEDQSLIQFVQEHANGKTLRLRECSFELAHLKMLVKILTKCPRLLSLELSSNSLNSQGLFSLLDSLAELSTIQTLTLRNNGLNSEQIEHFVKQFSHCNQHRDIRIEESWMTGSAAIGLIARCLSLQQHIREIRVHRASINMIIEANSISSSTSVGHSENVLPALQSISFDDCDVEGQHLTQLALPIQKCHALLDLKFSPLKLDRIGAEFFSAVIPFLRNLRNITLDLKGAAEEEVETLAEALPNTPAFESLSLSQLVLSDRGATLFGKAFQNVPNLKSFSMLQCSGWTAARGCDLVRGLMQCVLLTEIRLESVVLDQEGINILAQGLSRLASLRRLSLNNITVVTSETFVNGTGILCLLASFKGFKQMEEIELESWRMGDGGTNELVKYIPSWTGLVKISLSENIVTDQAGEKLLTALSHCRALQQLKLSKNKLGQASAAKLAKILPLLPQLSELNLSENQFGPQGSSVLCEGLVSMKALKKLHLTSVESSDLVGVASSLKHCPSIEDVSLSWNGCGDSLARTLAEILPLCTKLKRLDLEANNITTAGATLIAKCLPLCPSIEVIRLWRNSIAKDDENLKDPKLNFSSV

Target ORF information:

RefSeq Version	XM_017352040.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352040.1

ORF Insert Sequence:

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTAAGTC ATGTTGATCT GTCTACAAAT 
GGGGCTTTGA TATTGGTCAG ATCTCTTGCC GATTGTCAGC GTGTCACTTC TGTCGAACTC 
AGGCACCAAG CTGAAGCATT CATCAAATTT GTTCCTGAGA AAGCTGAAGT AGCAATTTGC 
AAGCTCACAG AGTATAATCT GAGCAAAACC AACGTCGTGA AGCTCTCGAA GATACTGGAG 
AGCTGTCCTC ATCTGTCTGA ACTGGACTTG TCTCATAACG TTCTCAGAGA TGAAGGTGTG 
AAGTGTTTTG TTGACCATTT GCCCAAACTA AAGATCTCCA GCTCAGTGAG CCTCATTGGC 
AACCGAATGA CTCAAATCGG GGCACTGCAT CTGGTCAACT CAATGAATAC CTGTGAAAAA 
GTTGTTGCTG TCGAAGTCAG TCTTGGAGTG GAGGATCAGT CTCTGATTCA GTTTGTGCAA 
GAACATGCCA ATGGCAAGAC TCTTAGGCTA AGGGAGTGCA GTTTTGAGCT TGCACACTTG 
AAGATGCTTG TGAAAATCCT GACAAAATGC CCAAGACTCC TTTCATTAGA ATTGTCTTCT 
AATTCACTGA ACAGTCAAGG ACTTTTTTCC TTACTGGATA GCCTGGCTGA ACTATCCACA 
ATCCAAACTT TAACGTTGAG AAATAATGGC CTGAATTCTG AGCAAATTGA GCATTTTGTT 
AAACAGTTTA GTCACTGCAA CCAACACAGA GACATCAGAA TCGAAGAGTC GTGGATGACA 
GGAAGTGCTG CTATTGGCCT CATTGCGAGA TGCCTCAGTC TACAGCAGCA CATCAGAGAA 
ATCAGGGTCC ACCGTGCATC TATAAACATG ATCATTGAAG CCAATTCCAT TTCCAGCTCC 
ACTTCAGTCG GTCATTCTGA AAATGTGTTG CCTGCTCTGC AGTCTATCAG CTTTGATGAC 
TGTGATGTAG AAGGACAGCA CCTCACTCAA CTCGCACTTC CCATCCAGAA ATGTCATGCC 
TTACTGGATT TAAAGTTCTC GCCACTTAAA TTGGACAGAA TAGGTGCAGA GTTTTTTTCT 
GCAGTCATTC CATTCTTAAG GAATCTCAGG AACATCACTC TGGATCTGAA AGGAGCCGCT 
GAGGAGGAAG TGGAAACTCT TGCTGAGGCC CTGCCAAACA CTCCAGCCTT TGAGAGTCTG 
AGCTTGTCCC AGCTTGTCTT AAGTGACCGT GGAGCTACAC TATTCGGAAA AGCTTTTCAA 
AATGTGCCAA ATCTGAAGTC ATTCAGTATG TTGCAGTGCT CTGGTTGGAC AGCAGCTCGA 
GGCTGTGACT TGGTCAGAGG CTTGATGCAG TGTGTTTTGC TGACAGAAAT ACGACTGGAG 
TCTGTGGTAC TCGACCAGGA GGGTATAAAT ATCCTAGCTC AAGGCTTGAG TCGACTGGCC 
TCACTTCGGA GGCTCAGCTT AAACAACATT ACAGTGGTGA CATCTGAGAC TTTTGTAAAT 
GGCACTGGAA TACTCTGTCT ACTGGCCTCT TTTAAAGGTT TCAAACAGAT GGAGGAGATT 
GAGTTGGAAA GTTGGCGGAT GGGAGATGGT GGTACCAATG AGCTTGTGAA ATACATACCT 
TCATGGACAG GGCTGGTAAA AATAAGTCTG TCAGAAAACA TTGTGACTGA CCAGGCCGGA 
GAGAAACTCC TCACAGCACT TTCCCATTGC AGAGCTCTTC AGCAGCTCAA GTTGTCTAAA 
AACAAGCTTG GTCAGGCCAG TGCTGCCAAG CTTGCTAAAA TTCTTCCATT GCTACCTCAG 
CTTTCAGAAC TCAATTTATC AGAGAATCAG TTTGGCCCAC AAGGATCAAG TGTTCTTTGT 
GAAGGTTTGG TGAGCATGAA GGCACTGAAA AAACTACATT TGACTTCGGT TGAGTCTTCA 
GATCTGGTTG GTGTGGCTTC ATCCTTAAAA CACTGTCCAT CCATAGAGGA TGTCAGTTTG 
TCATGGAATG GATGTGGTGA CAGTCTTGCA CGGACACTGG CTGAGATTCT TCCCTTGTGT 
ACGAAGCTGA AAAGACTCGA CCTGGAGGCT AATAACATAA CCACAGCGGG AGCCACACTG 
ATTGCCAAAT GCCTCCCATT ATGCCCATCC ATTGAAGTTA TAAGACTCTG GAGGAACTCA 
ATTGCCAAAG ATGATGAAAA TCTGAAAGAT CCCAAACTCA ACTTTTCATC GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlrc5 ( XM_017352039.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207528.1 Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23318
Clone ID Related Accession (Same CDS sequence)	XM_017352039.1 , XM_003200446.4 , XM_003200446.5
Accession Version	XM_017352039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207528.1
CDS	84..5324
Translation	MRMERMDWSGDALTLVAQEASVLVDLLCEQDSSVLDHIFDLLESNTQDEIRSLINNRDRVSAIVDYFKTSDNITCRKFFCTIYEYCKDIPFYLETTLVSIAGDPIDNYSSSRNVKRLRLDPLQSYTDALKSMVKEKHKTVMQSVVKDIRLDDTWVYLKHRNPPRAKDRTLQLQASLDSHEGEESEHKVSVESLLKTTGRVVVLLGQAGSGKTLLVHCLGHSWAENTFPSIQLLFLLEFRQLNLISRDLSLKELLFLFYPACSYVEEEKEEVFAFILTHPDKVCFILDGYDEFRAKLTDPRELESTVDLSTPMPMADLLSGLCSRKILPDCTVLVTCRPRDVTDMFGSPGLITCELQGFDRLGVKEYAEQYFHEKGDLKTNAVNLLMDNRHLLSMSHVPGLCHICCICVDHLFSSGEVLNHQLPTSLTQIYIQILLAFLSRFNEDGVSKTSLLMRYRTKIVEMSQLALKGLEGSTIVFMETEVSKELQDFGVRSGILSRVKLTHEDGSSGYGFTFMHLTMQEFLAALHLMTSQTITDGQLKKKLNLKTRWTTKTDPKSVFTDSVHLYVCGLAAEACTSCLVQLKGAESETTWVKKRQAAVRNVLLGLAGGASHTGPKIVELCRCAHETQDAKLAKAIGSRPQIELRNIRLNAVDMDSLAFVTAAADQTVCLDFGGCSIDLDCLEILPSFKNVDHLIFRSRKYDDKFAETLCGILSKLQALQQLDFISGGLTDVGAAKLAKALEDCPNITHLNVSDNSLKDEGIREIAETVSRLHNISSILMGKNNISTDGILTLIERMAACTSVQEVHVEGNKEINLSFSKRSDTISESKDLKNTEETKNIILKQCNLTVSHVASLCNKLRGFAILTLLDLSNNSLGNKGLKKILDLLPKLGGIQEINVSENGVDMEGVVMLASALCKQYNLKRVDASENGKKKVVLGFDTTRGHNSGELTQNETGMQLHKKLSLTHSDIQPAYMTKLCKQLVKCKNLLDIDFSNLKLTDDSVENLMLILPVMLSLHVLNLSHVDLSTNGALILVRSLADCQRVTSVELRHQAEAFIKFVPEKAEVAICKLTEYNLSKTNVVKLSKILESCPHLSELDLSHNVLRDEGVKCFVDHLPKLKISSSVSLIGNRMTQIGALHLVNSMNTCEKVVAVEVSLGVEDQSLIQFVQEHANGKTLRLRECSFELAHLKMLVKILTKCPRLLSLELSSNSLNSQGLFSLLDSLAELSTIQTLTLRNNGLNSEQIEHFVKQFSHCNQHRDIRIEESWMTGSAAIGLIARCLSLQQHIREIRVHRASINMIIEANSISSSTSVGHSENVLPALQSISFDDCDVEGQHLTQLALPIQKCHALLDLKFSPLKLDRIGAEFFSAVIPFLRNLRNITLDLKGAAEEEVETLAEALPNTPAFESLSLSQLVLSDRGATLFGKAFQNVPNLKSFSMLQCSGWTAARGCDLVRGLMQCVLLTEIRLESVVLDQEGINILAQGLSRLASLRRLSLNNITVVTSETFVNGTGILCLLASFKGFKQMEEIELESWRMGDGGTNELVKYIPSWTGLVKISLSENIVTDQAGEKLLTALSHCRALQQLKLSKNKLGQASAAKLAKILPLLPQLSELNLSENQFGPQGSSVLCEGLVSMKALKKLHLTSVESSDLVGVASSLKHCPSIEDVSLSWNGCGDSLARTLAEILPLCTKLKRLDLEANNITTAGATLIAKCLPLCPSIEVIRLWRNSIAKDDENLKDPKLNFSSV

Target ORF information:

RefSeq Version	XM_017352039.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352039.1

ORF Insert Sequence:

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlrc5 ( XM_003200446.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200494.2 Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X1, mRNA.

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CloneID	ODa23318
Clone ID Related Accession (Same CDS sequence)	XM_017352039.1 , XM_003200446.4 , XM_003200446.5
Accession Version	XM_003200446.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200446.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200494.2
CDS	504..5744
Translation	MRMERMDWSGDALTLVAQEASVLVDLLCEQDSSVLDHIFDLLESNTQDEIRSLINNRDRVSAIVDYFKTSDNITCRKFFCTIYEYCKDIPFYLETTLVSIAGDPIDNYSSSRNVKRLRLDPLQSYTDALKSMVKEKHKTVMQSVVKDIRLDDTWVYLKHRNPPRAKDRTLQLQASLDSHEGEESEHKVSVESLLKTTGRVVVLLGQAGSGKTLLVHCLGHSWAENTFPSIQLLFLLEFRQLNLISRDLSLKELLFLFYPACSYVEEEKEEVFAFILTHPDKVCFILDGYDEFRAKLTDPRELESTVDLSTPMPMADLLSGLCSRKILPDCTVLVTCRPRDVTDMFGSPGLITCELQGFDRLGVKEYAEQYFHEKGDLKTNAVNLLMDNRHLLSMSHVPGLCHICCICVDHLFSSGEVLNHQLPTSLTQIYIQILLAFLSRFNEDGVSKTSLLMRYRTKIVEMSQLALKGLEGSTIVFMETEVSKELQDFGVRSGILSRVKLTHEDGSSGYGFTFMHLTMQEFLAALHLMTSQTITDGQLKKKLNLKTRWTTKTDPKSVFTDSVHLYVCGLAAEACTSCLVQLKGAESETTWVKKRQAAVRNVLLGLAGGASHTGPKIVELCRCAHETQDAKLAKAIGSRPQIELRNIRLNAVDMDSLAFVTAAADQTVCLDFGGCSIDLDCLEILPSFKNVDHLIFRSRKYDDKFAETLCGILSKLQALQQLDFISGGLTDVGAAKLAKALEDCPNITHLNVSDNSLKDEGIREIAETVSRLHNISSILMGKNNISTDGILTLIERMAACTSVQEVHVEGNKEINLSFSKRSDTISESKDLKNTEETKNIILKQCNLTVSHVASLCNKLRGFAILTLLDLSNNSLGNKGLKKILDLLPKLGGIQEINVSENGVDMEGVVMLASALCKQYNLKRVDASENGKKKVVLGFDTTRGHNSGELTQNETGMQLHKKLSLTHSDIQPAYMTKLCKQLVKCKNLLDIDFSNLKLTDDSVENLMLILPVMLSLHVLNLSHVDLSTNGALILVRSLADCQRVTSVELRHQAEAFIKFVPEKAEVAICKLTEYNLSKTNVVKLSKILESCPHLSELDLSHNVLRDEGVKCFVDHLPKLKISSSVSLIGNRMTQIGALHLVNSMNTCEKVVAVEVSLGVEDQSLIQFVQEHANGKTLRLRECSFELAHLKMLVKILTKCPRLLSLELSSNSLNSQGLFSLLDSLAELSTIQTLTLRNNGLNSEQIEHFVKQFSHCNQHRDIRIEESWMTGSAAIGLIARCLSLQQHIREIRVHRASINMIIEANSISSSTSVGHSENVLPALQSISFDDCDVEGQHLTQLALPIQKCHALLDLKFSPLKLDRIGAEFFSAVIPFLRNLRNITLDLKGAAEEEVETLAEALPNTPAFESLSLSQLVLSDRGATLFGKAFQNVPNLKSFSMLQCSGWTAARGCDLVRGLMQCVLLTEIRLESVVLDQEGINILAQGLSRLASLRRLSLNNITVVTSETFVNGTGILCLLASFKGFKQMEEIELESWRMGDGGTNELVKYIPSWTGLVKISLSENIVTDQAGEKLLTALSHCRALQQLKLSKNKLGQASAAKLAKILPLLPQLSELNLSENQFGPQGSSVLCEGLVSMKALKKLHLTSVESSDLVGVASSLKHCPSIEDVSLSWNGCGDSLARTLAEILPLCTKLKRLDLEANNITTAGATLIAKCLPLCPSIEVIRLWRNSIAKDDENLKDPKLNFSSV

Target ORF information:

RefSeq Version	XM_003200446.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-122c9.5 (si:ch211-122c9.5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200446.4

ORF Insert Sequence:

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlrc5 ( XM_003200446.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200494.2 Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X1, mRNA.

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CloneID	ODa23318
Clone ID Related Accession (Same CDS sequence)	XM_017352039.1 , XM_003200446.4 , XM_003200446.5
Accession Version	XM_003200446.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200446.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200494.2
CDS	508..5748
Translation	MRMERMDWSGDALTLVAQEASVLVDLLCEQDSSVLDHIFDLLESNTQDEIRSLINNRDRVSAIVDYFKTSDNITCRKFFCTIYEYCKDIPFYLETTLVSIAGDPIDNYSSSRNVKRLRLDPLQSYTDALKSMVKEKHKTVMQSVVKDIRLDDTWVYLKHRNPPRAKDRTLQLQASLDSHEGEESEHKVSVESLLKTTGRVVVLLGQAGSGKTLLVHCLGHSWAENTFPSIQLLFLLEFRQLNLISRDLSLKELLFLFYPACSYVEEEKEEVFAFILTHPDKVCFILDGYDEFRAKLTDPRELESTVDLSTPMPMADLLSGLCSRKILPDCTVLVTCRPRDVTDMFGSPGLITCELQGFDRLGVKEYAEQYFHEKGDLKTNAVNLLMDNRHLLSMSHVPGLCHICCICVDHLFSSGEVLNHQLPTSLTQIYIQILLAFLSRFNEDGVSKTSLLMRYRTKIVEMSQLALKGLEGSTIVFMETEVSKELQDFGVRSGILSRVKLTHEDGSSGYGFTFMHLTMQEFLAALHLMTSQTITDGQLKKKLNLKTRWTTKTDPKSVFTDSVHLYVCGLAAEACTSCLVQLKGAESETTWVKKRQAAVRNVLLGLAGGASHTGPKIVELCRCAHETQDAKLAKAIGSRPQIELRNIRLNAVDMDSLAFVTAAADQTVCLDFGGCSIDLDCLEILPSFKNVDHLIFRSRKYDDKFAETLCGILSKLQALQQLDFISGGLTDVGAAKLAKALEDCPNITHLNVSDNSLKDEGIREIAETVSRLHNISSILMGKNNISTDGILTLIERMAACTSVQEVHVEGNKEINLSFSKRSDTISESKDLKNTEETKNIILKQCNLTVSHVASLCNKLRGFAILTLLDLSNNSLGNKGLKKILDLLPKLGGIQEINVSENGVDMEGVVMLASALCKQYNLKRVDASENGKKKVVLGFDTTRGHNSGELTQNETGMQLHKKLSLTHSDIQPAYMTKLCKQLVKCKNLLDIDFSNLKLTDDSVENLMLILPVMLSLHVLNLSHVDLSTNGALILVRSLADCQRVTSVELRHQAEAFIKFVPEKAEVAICKLTEYNLSKTNVVKLSKILESCPHLSELDLSHNVLRDEGVKCFVDHLPKLKISSSVSLIGNRMTQIGALHLVNSMNTCEKVVAVEVSLGVEDQSLIQFVQEHANGKTLRLRECSFELAHLKMLVKILTKCPRLLSLELSSNSLNSQGLFSLLDSLAELSTIQTLTLRNNGLNSEQIEHFVKQFSHCNQHRDIRIEESWMTGSAAIGLIARCLSLQQHIREIRVHRASINMIIEANSISSSTSVGHSENVLPALQSISFDDCDVEGQHLTQLALPIQKCHALLDLKFSPLKLDRIGAEFFSAVIPFLRNLRNITLDLKGAAEEEVETLAEALPNTPAFESLSLSQLVLSDRGATLFGKAFQNVPNLKSFSMLQCSGWTAARGCDLVRGLMQCVLLTEIRLESVVLDQEGINILAQGLSRLASLRRLSLNNITVVTSETFVNGTGILCLLASFKGFKQMEEIELESWRMGDGGTNELVKYIPSWTGLVKISLSENIVTDQAGEKLLTALSHCRALQQLKLSKNKLGQASAAKLAKILPLLPQLSELNLSENQFGPQGSSVLCEGLVSMKALKKLHLTSVESSDLVGVASSLKHCPSIEDVSLSWNGCGDSLARTLAEILPLCTKLKRLDLEANNITTAGATLIAKCLPLCPSIEVIRLWRNSIAKDDENLKDPKLNFSSV

Target ORF information:

RefSeq Version	XM_003200446.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NLR family, CARD domain containing 5 (nlrc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200446.5

ORF Insert Sequence:

ATGAGGATGG AGAGGATGGA CTGGTCGGGG GACGCCCTGA CACTGGTAGC CCAAGAGGCC 
AGTGTACTGG TGGACTTGCT CTGCGAACAG GATAGTAGTG TTTTGGACCA TATCTTTGAC 
TTGTTGGAAT CTAACACACA GGATGAGATA CGATCACTGA TCAACAATAG AGATCGTGTC 
TCAGCCATCG TGGATTACTT CAAGACTTCA GACAATATCA CATGTAGAAA GTTTTTCTGC 
ACCATATATG AGTACTGTAA AGACATCCCT TTCTATCTTG AAACTACACT AGTGTCAATA 
GCTGGAGATC CAATTGATAA TTATTCATCA TCAAGAAATG TGAAACGTCT ACGACTAGAT 
CCTTTACAGA GCTACACAGA TGCTCTGAAG TCCATGGTAA AGGAAAAACA TAAGACAGTG 
ATGCAGAGTG TGGTAAAGGA TATACGTCTA GATGACACAT GGGTGTATTT AAAGCACAGG 
AACCCTCCAC GAGCAAAAGA CAGGACCCTC CAGCTTCAAG CCTCTCTAGA CAGCCATGAA 
GGTGAAGAGT CAGAGCACAA AGTGTCCGTT GAGTCTCTTT TGAAAACCAC TGGCAGGGTT 
GTTGTGCTGC TGGGCCAGGC TGGATCTGGT AAAACCCTGC TCGTGCACTG CCTCGGCCAT 
AGCTGGGCTG AAAACACCTT CCCGTCTATT CAGCTGCTGT TTCTGCTGGA GTTCCGGCAG 
CTCAATCTGA TCTCTCGAGA CCTGTCCTTG AAAGAATTAC TTTTTCTCTT CTATCCTGCT 
TGTTCGTATG TTGAAGAAGA AAAAGAAGAA GTGTTTGCAT TCATCCTCAC ACACCCGGAC 
AAAGTCTGCT TCATATTAGA TGGTTACGAT GAGTTTAGAG CCAAACTCAC AGATCCTAGA 
GAACTTGAGA GCACAGTTGA TCTGAGCACA CCCATGCCAA TGGCCGATCT ACTTTCAGGA 
CTCTGCAGTC GTAAAATTCT TCCAGATTGC ACTGTTTTGG TCACCTGCAG ACCAAGAGAT 
GTGACGGACA TGTTTGGAAG CCCTGGGCTC ATAACCTGTG AGCTTCAGGG GTTTGACCGA 
TTGGGGGTGA AGGAATACGC TGAGCAGTAC TTCCATGAAA AAGGAGATCT CAAAACAAAT 
GCTGTTAACT TGTTGATGGA TAACCGTCAC CTCTTGTCCA TGTCTCATGT GCCTGGACTC 
TGTCATATCT GCTGTATATG TGTGGATCAT CTGTTCTCCA GTGGTGAAGT GTTGAATCAT 
CAGCTTCCAA CGTCCTTAAC CCAAATCTAC ATCCAGATTC TGTTGGCATT CCTCAGTCGG 
TTCAATGAAG ATGGAGTGTC TAAAACATCT CTGCTTATGA GATACAGGAC TAAAATTGTT 
GAGATGAGCC AACTGGCTCT CAAAGGACTG GAAGGAAGCA CCATAGTCTT TATGGAAACA 
GAAGTATCCA AGGAACTCCA AGATTTTGGT GTCAGAAGTG GGATACTGTC TCGCGTAAAG 
CTGACGCATG AGGATGGATC TTCAGGATAC GGTTTTACCT TTATGCACCT TACAATGCAA 
GAGTTTCTCG CTGCTCTCCA CCTCATGACT AGTCAGACAA TCACAGATGG TCAACTTAAG 
AAGAAGCTTA ACTTGAAAAC CAGATGGACT ACCAAGACTG ACCCAAAATC CGTCTTTACA 
GACTCTGTCC ATCTTTATGT ATGTGGGCTT GCAGCAGAGG CCTGTACTTC CTGTTTGGTG 
CAACTTAAAG GAGCAGAGAG TGAGACGACT TGGGTAAAAA AGCGACAAGC TGCTGTTCGT 
AATGTTTTAC TAGGTTTAGC GGGAGGTGCC AGTCATACAG GTCCAAAAAT TGTGGAGCTG 
TGCCGATGTG CTCATGAGAC ACAGGATGCG AAACTGGCAA AGGCTATTGG TTCGAGACCA 
CAGATTGAAC TGCGAAATAT CAGACTGAAT GCTGTTGACA TGGATTCCCT TGCATTTGTA 
ACAGCGGCTG CTGATCAGAC TGTATGTCTA GACTTTGGTG GATGTTCAAT TGACTTGGAT 
TGTTTGGAAA TCCTCCCTAG TTTCAAGAAT GTGGATCACC TCATTTTCCG TAGCCGGAAA 
TATGATGACA AATTTGCAGA GACTTTGTGC GGTATCCTGT CTAAACTCCA GGCCTTGCAA 
CAATTAGATT TTATCAGTGG AGGCCTCACC GATGTTGGAG CGGCTAAACT TGCCAAGGCG 
TTGGAGGATT GTCCAAACAT CACACACCTG AATGTCAGTG ATAACTCTTT GAAAGATGAG 
GGTATAAGAG AGATTGCAGA AACAGTGTCC AGGCTGCACA ACATCTCCTC TATTTTAATG 
GGGAAGAATA ACATTTCCAC AGATGGGATC CTCACTCTCA TTGAGAGAAT GGCCGCTTGC 
ACCTCTGTTC AGGAAGTCCA TGTTGAGGGG AATAAAGAGA TCAACCTTAG CTTCTCAAAA 
CGCTCTGACA CAATCAGTGA AAGCAAAGAC TTGAAAAACA CAGAAGAGAC AAAGAATATA 
ATCTTGAAGC AGTGTAACCT CACAGTTTCC CATGTGGCCA GCTTGTGTAA TAAACTCAGA 
GGCTTTGCCA TCCTAACGCT TTTAGATTTG TCCAACAACT CTCTGGGTAA CAAAGGCCTG 
AAGAAAATTT TGGACCTTCT GCCTAAACTA GGAGGAATTC AGGAGATAAA TGTCAGTGAA 
AATGGTGTTG ATATGGAGGG TGTGGTGATG CTGGCTTCTG CCCTCTGTAA ACAGTACAAC 
TTAAAAAGAG TGGATGCAAG TGAAAATGGA AAGAAAAAAG TGGTTCTTGG GTTTGACACC 
ACCAGAGGGC ATAATTCAGG GGAGCTGACT CAAAATGAAA CCGGGATGCA ACTGCACAAA 
AAACTGAGTT TAACCCATAG TGATATCCAA CCTGCCTACA TGACCAAGCT GTGTAAGCAA 
CTGGTGAAAT GCAAAAACCT ACTGGACATT GACTTCTCCA ATCTAAAATT AACCGACGAT 
TCTGTTGAGA ATTTGATGTT GATCCTTCCT GTCATGTTGT CTCTTCATGT ACTGAACTTA 
AGTCATGTTG ATCTGTCTAC AAATGGGGCT TTGATATTGG TCAGATCTCT TGCCGATTGT 
CAGCGTGTCA CTTCTGTCGA ACTCAGGCAC CAAGCTGAAG CATTCATCAA ATTTGTTCCT 
GAGAAAGCTG AAGTAGCAAT TTGCAAGCTC ACAGAGTATA ATCTGAGCAA AACCAACGTC 
GTGAAGCTCT CGAAGATACT GGAGAGCTGT CCTCATCTGT CTGAACTGGA CTTGTCTCAT 
AACGTTCTCA GAGATGAAGG TGTGAAGTGT TTTGTTGACC ATTTGCCCAA ACTAAAGATC 
TCCAGCTCAG TGAGCCTCAT TGGCAACCGA ATGACTCAAA TCGGGGCACT GCATCTGGTC 
AACTCAATGA ATACCTGTGA AAAAGTTGTT GCTGTCGAAG TCAGTCTTGG AGTGGAGGAT 
CAGTCTCTGA TTCAGTTTGT GCAAGAACAT GCCAATGGCA AGACTCTTAG GCTAAGGGAG 
TGCAGTTTTG AGCTTGCACA CTTGAAGATG CTTGTGAAAA TCCTGACAAA ATGCCCAAGA 
CTCCTTTCAT TAGAATTGTC TTCTAATTCA CTGAACAGTC AAGGACTTTT TTCCTTACTG 
GATAGCCTGG CTGAACTATC CACAATCCAA ACTTTAACGT TGAGAAATAA TGGCCTGAAT 
TCTGAGCAAA TTGAGCATTT TGTTAAACAG TTTAGTCACT GCAACCAACA CAGAGACATC 
AGAATCGAAG AGTCGTGGAT GACAGGAAGT GCTGCTATTG GCCTCATTGC GAGATGCCTC 
AGTCTACAGC AGCACATCAG AGAAATCAGG GTCCACCGTG CATCTATAAA CATGATCATT 
GAAGCCAATT CCATTTCCAG CTCCACTTCA GTCGGTCATT CTGAAAATGT GTTGCCTGCT 
CTGCAGTCTA TCAGCTTTGA TGACTGTGAT GTAGAAGGAC AGCACCTCAC TCAACTCGCA 
CTTCCCATCC AGAAATGTCA TGCCTTACTG GATTTAAAGT TCTCGCCACT TAAATTGGAC 
AGAATAGGTG CAGAGTTTTT TTCTGCAGTC ATTCCATTCT TAAGGAATCT CAGGAACATC 
ACTCTGGATC TGAAAGGAGC CGCTGAGGAG GAAGTGGAAA CTCTTGCTGA GGCCCTGCCA 
AACACTCCAG CCTTTGAGAG TCTGAGCTTG TCCCAGCTTG TCTTAAGTGA CCGTGGAGCT 
ACACTATTCG GAAAAGCTTT TCAAAATGTG CCAAATCTGA AGTCATTCAG TATGTTGCAG 
TGCTCTGGTT GGACAGCAGC TCGAGGCTGT GACTTGGTCA GAGGCTTGAT GCAGTGTGTT 
TTGCTGACAG AAATACGACT GGAGTCTGTG GTACTCGACC AGGAGGGTAT AAATATCCTA 
GCTCAAGGCT TGAGTCGACT GGCCTCACTT CGGAGGCTCA GCTTAAACAA CATTACAGTG 
GTGACATCTG AGACTTTTGT AAATGGCACT GGAATACTCT GTCTACTGGC CTCTTTTAAA 
GGTTTCAAAC AGATGGAGGA GATTGAGTTG GAAAGTTGGC GGATGGGAGA TGGTGGTACC 
AATGAGCTTG TGAAATACAT ACCTTCATGG ACAGGGCTGG TAAAAATAAG TCTGTCAGAA 
AACATTGTGA CTGACCAGGC CGGAGAGAAA CTCCTCACAG CACTTTCCCA TTGCAGAGCT 
CTTCAGCAGC TCAAGTTGTC TAAAAACAAG CTTGGTCAGG CCAGTGCTGC CAAGCTTGCT 
AAAATTCTTC CATTGCTACC TCAGCTTTCA GAACTCAATT TATCAGAGAA TCAGTTTGGC 
CCACAAGGAT CAAGTGTTCT TTGTGAAGGT TTGGTGAGCA TGAAGGCACT GAAAAAACTA 
CATTTGACTT CGGTTGAGTC TTCAGATCTG GTTGGTGTGG CTTCATCCTT AAAACACTGT 
CCATCCATAG AGGATGTCAG TTTGTCATGG AATGGATGTG GTGACAGTCT TGCACGGACA 
CTGGCTGAGA TTCTTCCCTT GTGTACGAAG CTGAAAAGAC TCGACCTGGA GGCTAATAAC 
ATAACCACAG CGGGAGCCAC ACTGATTGCC AAATGCCTCC CATTATGCCC ATCCATTGAA 
GTTATAAGAC TCTGGAGGAA CTCAATTGCC AAAGATGATG AAAATCTGAA AGATCCCAAA 
CTCAACTTTT CATCGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nlrc5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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