LOC100334178 cDNA ORF clone, Danio rerio(zebrafish)

The following LOC100334178 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100334178 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa56440 XM_009298565.3
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Danio rerio E3 ubiquitin-protein ligase RNF31 (LOC100334178), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
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ODa56440 XM_009298565.2 Danio rerio E3 ubiquitin-protein ligase RNF31 (LOC100334178), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa56440
    Clone ID Related Accession (Same CDS sequence) XM_009298565.2 , XM_009298565.3
    Accession Version XM_009298565.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase RNF31
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) and transcript sequence (GDQH01015019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 742 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCTCTC TCACTGATCA GCTGGAGGAG GTGAGGGTGA ATGCGGAGGG CAGTTTATCT 
    TCTCCAAGTT CTGCCCAGGA GATGAGGGCA GGGGTGGAAG CTATGGCCAA CATCCCCCTT
    CCACCCTCCA GCAAGTATCG CTGTATAGCT GCCGAAGTCA TGTTGACAGA GAACGGTAGT
    GGCAATAACA GGAAGGAGTC CCTAGCCCTT CTGCAGAAAC TTTCTACTGC TCTTAACATT
    TTGGAGAAGT ATGGCTCCAA TCTTACCAAC CCCAGCAGAC CTAAGTACTG GCGAACAGTA
    AAGTACAACA ATCCTGTCTT CAGGACCACC GTAGACTCTA TTCAGGGTGG CAGAGCAGTA
    CTTAACCTGT ACGGCTACAC CAATCAGCAG CAGGATGGCT TAAGCTTTCC TGATGATGTT
    GTTGAGCCAG ATGTTGGCAA GGTGGCGTCA GTAACTCTTG AGGTCATGTG TCTTCGAATG
    GAGCTGGACA TGCTAACCAA GGGCACCCAT CCTCATCCAG AGTTTTTTGA AAGACTTATT
    CCCTCTCTCA CCGTGCAGGA GGAAGAGGGA CTCAGCACAG ATGCAGTTGT CTTTGCTCCT
    AGTGAGAAAG TATGGGATAA ACAAACTCCT TCACCTCTGT CTAGTCCTCC TCAGGCTAAA
    TCCCCTTTCA GTCCAGCTCT CTCTCTCTCT TCTCTGGCCC CCTCTACACA AACAGAGAAC
    TGCACCATAT GTGGCATCTT TCCAGTGTCC GCTCACTGTC CCACTTGTAC CCAGAAGCTT
    TGCACAGAAT GTGACAGACT CTATCACTCT CATTCTGGAC GATCTACACA CATAAGAATC
    CCTGTGACTT CCTCAAGACC CACAAAGCGA CTCAGCTCAT CACTGTCAAC ATGGCAGTGT
    TCATACTGCA CTACAGTAAA TTCGATGCTG CAGGTTTTGT GTGAAACCTG CGATCGCCCC
    CGTTTGTCCT CTGCTGCTCC ATCACTACAG GAGGAGTCAT CACAGCCCTC TACAATATCT
    GAGTGGCAGT GTAAAAGCTG CACAGTGGTG AATGCAGGTA GCAGTATTTT ATGTGAGGTT
    TGCGAACGAC CTCGTCTGGC CACCAGACCA CCTGCTGTAC CCCCACGAGC AACCCCTTCC
    TCCTCAGCAC TAATGGACAG CCAGTGGATA TGCCAGTTTT GTACATACGT CAACCGGACA
    CCATCGACCG TATGTGACAT GTGTTGTCTA TCAAGACCAG AGCCTTCCAT ATCACCCTCT
    AAGCCCCTCC CACTATCACC AGTCAAGGAC CTTCTACCTC CTGCAGCCAC AACCAAAGCC
    ACGCCCACTG AAGACCCTGA TTTAAAGCGA CAGAGACTGA TGAAGGAGGA CGGGCTCAAA
    CTTATTCAGC TCATTCGAGA GGGAGAGAAG AAAGGAGTGA GCCCAGAGGA GGTTTACACC
    AGCATGCGAG TGTCAGGAAA CAGTAACGTA ACGCCGTATG ATTGGCTGAA GACAGAGCTT
    CCTCATGTGC TTGATGAGAT CTGTGTGATG GCTGCATCGT GTCAGCAGGA GCCCAACGGT
    TCAGGGGGGA ACAATGGCCA CGATGGACTA AAGGGCAACC CTGTGCCCCT GTCCAGAGCG
    GAAGCTAAAC GTGCCTGGCT GGCAGCAGGG GGCGATAATG AAAAGGCCGT CAGACAGGCT
    CTCAGAGACC GCAGAGTTAA AGTAAAAGAG CTTGGCTCTT TGGGCTTTAA TGATGCAACC
    CGCTGTGAAG AAGCACTGAG GCAGAGTGGA GGCGATGTAA GGGGGGCTCT AGTCCTGCTG
    CAGCGCCCTT TGCTGGAGCC CTTCCATCAG CGCATGTGGA GCGAAGAACC AGAGCCCCCC
    ATCGACATCA ACCACCCAGA TAAACAGCAT GTTTGCCGTA GGCTGCTAGC AGTGTTTGAT
    CTGCCCAGCT GGGGGCGCTG TGAGCTGATT TTGTCTTTGC TACGGGACCC TGCAGCCAAT
    TACACACTGG AAGACGTGGT TCAGGCTGTG CGTGAATCAC CTGACCGAGA CTTCATTCGG
    CGCGTGCTGA ACAAAGAATG CCCCATCTGC CTGTCAGTTT TTCCCCACAG CAAGATGCGG
    TCTCTGACAT CATGCCAGTG CTCAGTGTGC TGTGGATGTT TCCAGCAGCA TTTTACCATA
    GCGGTGAGAG ACAAACACAT CAGAGACATG GTGTGTCCTG TCTGCACAGA GCCTGACATC
    AACGACCCAG AGCACCTTAA CAGCTACTTC TCTACACTGG ACATACAGCT CAGGGATTGT
    CTTGAACAAG AGGTGTACGA GCTCTTCCAC AAGAAGCTAA CTGAGCAGGC TCTCATCAAG
    GACCCAAAGT TTCTGTGGTG CAGTCATTGC TCATATGGCT TCATCTATGA CGACGATCAA
    CTCAAGGTCA CCTGTAGCCA GTGCAGGAAA AGCTTTTGTG CTCAGTGCAA AAAAACATGG
    GAGCCACAGC ACATGGGTCT TTCGTGTGAG CAATTCCAGC TATGGAAGCG AGAGAATGAC
    CCTGAATACC AGAGGCAGGG TTTGGCCGGA TACCTGCGTG ACAACGGCAT CACTTGTCCT
    AACTGTAGGT TCCAGTATGC TCTGGCCAGG GGCGGCTGCA TGCACTTCAG CTGTTCTCAG
    TGCAGATATC AGTTCTGCAG TGGATGTAAC AACCCCTTCC ACACAACATG TGCAGTGAAC
    CAGTGTACTG TGACAGGTCT GCATGCCCAT CACCCCAGAG ACTGCCTCTT CTACCTCCGA
    GACTGGGAGC CTGCAAGGCT TCAGGCCTTA CTGCAGAAAA AAGGGGTGAA CTACAATACT
    GAAACTGCAC CAGGAGCACA GACTGGTGTG TGTGGAGTGA TCGAGCAGAA AGATGAAGGG
    CCTCGTCCGA CCGATTCTCC GTGTGGTGCT CAGACTCAGC CGGGCCAGGC GGGACTCTGC
    GATAAGCATT ATCGAGAGTA CCTGGTCAGC CTCATAAATG GCCATTCAAT TGATCCGGCT
    CTTCTTTTCA CAATGGACGA GCTAACAGTA GCCTGCAGGA GATACCATGT GGACATCACA
    CTGCTGGATA GAGAAGATGA ACAAACACAC TTTAGCAGAC TGCTTAAGAA ACTCATGGAT
    GACGTGCCTC TGGGTGACAA GGTCCTTCGC AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296840.2
    CDS79..3237
    Translation

    Target ORF information:

    RefSeq Version XM_009298565.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio E3 ubiquitin-protein ligase RNF31 (LOC100334178), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCTCTC TCACTGATCA GCTGGAGGAG GTGAGGGTGA ATGCGGAGGG CAGTTTATCT 
    TCTCCAAGTT CTGCCCAGGA GATGAGGGCA GGGGTGGAAG CTATGGCCAA CATCCCCCTT
    CCACCCTCCA GCAAGTATCG CTGTATAGCT GCCGAAGTCA TGTTGACAGA GAACGGTAGT
    GGCAATAACA GGAAGGAGTC CCTAGCCCTT CTGCAGAAAC TTTCTACTGC TCTTAACATT
    TTGGAGAAGT ATGGCTCCAA TCTTACCAAC CCCAGCAGAC CTAAGTACTG GCGAACAGTA
    AAGTACAACA ATCCTGTCTT CAGGACCACC GTAGACTCTA TTCAGGGTGG CAGAGCAGTA
    CTTAACCTGT ACGGCTACAC CAATCAGCAG CAGGATGGCT TAAGCTTTCC TGATGATGTT
    GTTGAGCCAG ATGTTGGCAA GGTGGCGTCA GTAACTCTTG AGGTCATGTG TCTTCGAATG
    GAGCTGGACA TGCTAACCAA GGGCACCCAT CCTCATCCAG AGTTTTTTGA AAGACTTATT
    CCCTCTCTCA CCGTGCAGGA GGAAGAGGGA CTCAGCACAG ATGCAGTTGT CTTTGCTCCT
    AGTGAGAAAG TATGGGATAA ACAAACTCCT TCACCTCTGT CTAGTCCTCC TCAGGCTAAA
    TCCCCTTTCA GTCCAGCTCT CTCTCTCTCT TCTCTGGCCC CCTCTACACA AACAGAGAAC
    TGCACCATAT GTGGCATCTT TCCAGTGTCC GCTCACTGTC CCACTTGTAC CCAGAAGCTT
    TGCACAGAAT GTGACAGACT CTATCACTCT CATTCTGGAC GATCTACACA CATAAGAATC
    CCTGTGACTT CCTCAAGACC CACAAAGCGA CTCAGCTCAT CACTGTCAAC ATGGCAGTGT
    TCATACTGCA CTACAGTAAA TTCGATGCTG CAGGTTTTGT GTGAAACCTG CGATCGCCCC
    CGTTTGTCCT CTGCTGCTCC ATCACTACAG GAGGAGTCAT CACAGCCCTC TACAATATCT
    GAGTGGCAGT GTAAAAGCTG CACAGTGGTG AATGCAGGTA GCAGTATTTT ATGTGAGGTT
    TGCGAACGAC CTCGTCTGGC CACCAGACCA CCTGCTGTAC CCCCACGAGC AACCCCTTCC
    TCCTCAGCAC TAATGGACAG CCAGTGGATA TGCCAGTTTT GTACATACGT CAACCGGACA
    CCATCGACCG TATGTGACAT GTGTTGTCTA TCAAGACCAG AGCCTTCCAT ATCACCCTCT
    AAGCCCCTCC CACTATCACC AGTCAAGGAC CTTCTACCTC CTGCAGCCAC AACCAAAGCC
    ACGCCCACTG AAGACCCTGA TTTAAAGCGA CAGAGACTGA TGAAGGAGGA CGGGCTCAAA
    CTTATTCAGC TCATTCGAGA GGGAGAGAAG AAAGGAGTGA GCCCAGAGGA GGTTTACACC
    AGCATGCGAG TGTCAGGAAA CAGTAACGTA ACGCCGTATG ATTGGCTGAA GACAGAGCTT
    CCTCATGTGC TTGATGAGAT CTGTGTGATG GCTGCATCGT GTCAGCAGGA GCCCAACGGT
    TCAGGGGGGA ACAATGGCCA CGATGGACTA AAGGGCAACC CTGTGCCCCT GTCCAGAGCG
    GAAGCTAAAC GTGCCTGGCT GGCAGCAGGG GGCGATAATG AAAAGGCCGT CAGACAGGCT
    CTCAGAGACC GCAGAGTTAA AGTAAAAGAG CTTGGCTCTT TGGGCTTTAA TGATGCAACC
    CGCTGTGAAG AAGCACTGAG GCAGAGTGGA GGCGATGTAA GGGGGGCTCT AGTCCTGCTG
    CAGCGCCCTT TGCTGGAGCC CTTCCATCAG CGCATGTGGA GCGAAGAACC AGAGCCCCCC
    ATCGACATCA ACCACCCAGA TAAACAGCAT GTTTGCCGTA GGCTGCTAGC AGTGTTTGAT
    CTGCCCAGCT GGGGGCGCTG TGAGCTGATT TTGTCTTTGC TACGGGACCC TGCAGCCAAT
    TACACACTGG AAGACGTGGT TCAGGCTGTG CGTGAATCAC CTGACCGAGA CTTCATTCGG
    CGCGTGCTGA ACAAAGAATG CCCCATCTGC CTGTCAGTTT TTCCCCACAG CAAGATGCGG
    TCTCTGACAT CATGCCAGTG CTCAGTGTGC TGTGGATGTT TCCAGCAGCA TTTTACCATA
    GCGGTGAGAG ACAAACACAT CAGAGACATG GTGTGTCCTG TCTGCACAGA GCCTGACATC
    AACGACCCAG AGCACCTTAA CAGCTACTTC TCTACACTGG ACATACAGCT CAGGGATTGT
    CTTGAACAAG AGGTGTACGA GCTCTTCCAC AAGAAGCTAA CTGAGCAGGC TCTCATCAAG
    GACCCAAAGT TTCTGTGGTG CAGTCATTGC TCATATGGCT TCATCTATGA CGACGATCAA
    CTCAAGGTCA CCTGTAGCCA GTGCAGGAAA AGCTTTTGTG CTCAGTGCAA AAAAACATGG
    GAGCCACAGC ACATGGGTCT TTCGTGTGAG CAATTCCAGC TATGGAAGCG AGAGAATGAC
    CCTGAATACC AGAGGCAGGG TTTGGCCGGA TACCTGCGTG ACAACGGCAT CACTTGTCCT
    AACTGTAGGT TCCAGTATGC TCTGGCCAGG GGCGGCTGCA TGCACTTCAG CTGTTCTCAG
    TGCAGATATC AGTTCTGCAG TGGATGTAAC AACCCCTTCC ACACAACATG TGCAGTGAAC
    CAGTGTACTG TGACAGGTCT GCATGCCCAT CACCCCAGAG ACTGCCTCTT CTACCTCCGA
    GACTGGGAGC CTGCAAGGCT TCAGGCCTTA CTGCAGAAAA AAGGGGTGAA CTACAATACT
    GAAACTGCAC CAGGAGCACA GACTGGTGTG TGTGGAGTGA TCGAGCAGAA AGATGAAGGG
    CCTCGTCCGA CCGATTCTCC GTGTGGTGCT CAGACTCAGC CGGGCCAGGC GGGACTCTGC
    GATAAGCATT ATCGAGAGTA CCTGGTCAGC CTCATAAATG GCCATTCAAT TGATCCGGCT
    CTTCTTTTCA CAATGGACGA GCTAACAGTA GCCTGCAGGA GATACCATGT GGACATCACA
    CTGCTGGATA GAGAAGATGA ACAAACACAC TTTAGCAGAC TGCTTAAGAA ACTCATGGAT
    GACGTGCCTC TGGGTGACAA GGTCCTTCGC AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56440
    Clone ID Related Accession (Same CDS sequence) XM_009298565.2 , XM_009298565.3
    Accession Version XM_009298565.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase RNF31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) and transcript sequence (GDQQ01015540.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 708 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCTCTC TCACTGATCA GCTGGAGGAG GTGAGGGTGA ATGCGGAGGG CAGTTTATCT 
    TCTCCAAGTT CTGCCCAGGA GATGAGGGCA GGGGTGGAAG CTATGGCCAA CATCCCCCTT
    CCACCCTCCA GCAAGTATCG CTGTATAGCT GCCGAAGTCA TGTTGACAGA GAACGGTAGT
    GGCAATAACA GGAAGGAGTC CCTAGCCCTT CTGCAGAAAC TTTCTACTGC TCTTAACATT
    TTGGAGAAGT ATGGCTCCAA TCTTACCAAC CCCAGCAGAC CTAAGTACTG GCGAACAGTA
    AAGTACAACA ATCCTGTCTT CAGGACCACC GTAGACTCTA TTCAGGGTGG CAGAGCAGTA
    CTTAACCTGT ACGGCTACAC CAATCAGCAG CAGGATGGCT TAAGCTTTCC TGATGATGTT
    GTTGAGCCAG ATGTTGGCAA GGTGGCGTCA GTAACTCTTG AGGTCATGTG TCTTCGAATG
    GAGCTGGACA TGCTAACCAA GGGCACCCAT CCTCATCCAG AGTTTTTTGA AAGACTTATT
    CCCTCTCTCA CCGTGCAGGA GGAAGAGGGA CTCAGCACAG ATGCAGTTGT CTTTGCTCCT
    AGTGAGAAAG TATGGGATAA ACAAACTCCT TCACCTCTGT CTAGTCCTCC TCAGGCTAAA
    TCCCCTTTCA GTCCAGCTCT CTCTCTCTCT TCTCTGGCCC CCTCTACACA AACAGAGAAC
    TGCACCATAT GTGGCATCTT TCCAGTGTCC GCTCACTGTC CCACTTGTAC CCAGAAGCTT
    TGCACAGAAT GTGACAGACT CTATCACTCT CATTCTGGAC GATCTACACA CATAAGAATC
    CCTGTGACTT CCTCAAGACC CACAAAGCGA CTCAGCTCAT CACTGTCAAC ATGGCAGTGT
    TCATACTGCA CTACAGTAAA TTCGATGCTG CAGGTTTTGT GTGAAACCTG CGATCGCCCC
    CGTTTGTCCT CTGCTGCTCC ATCACTACAG GAGGAGTCAT CACAGCCCTC TACAATATCT
    GAGTGGCAGT GTAAAAGCTG CACAGTGGTG AATGCAGGTA GCAGTATTTT ATGTGAGGTT
    TGCGAACGAC CTCGTCTGGC CACCAGACCA CCTGCTGTAC CCCCACGAGC AACCCCTTCC
    TCCTCAGCAC TAATGGACAG CCAGTGGATA TGCCAGTTTT GTACATACGT CAACCGGACA
    CCATCGACCG TATGTGACAT GTGTTGTCTA TCAAGACCAG AGCCTTCCAT ATCACCCTCT
    AAGCCCCTCC CACTATCACC AGTCAAGGAC CTTCTACCTC CTGCAGCCAC AACCAAAGCC
    ACGCCCACTG AAGACCCTGA TTTAAAGCGA CAGAGACTGA TGAAGGAGGA CGGGCTCAAA
    CTTATTCAGC TCATTCGAGA GGGAGAGAAG AAAGGAGTGA GCCCAGAGGA GGTTTACACC
    AGCATGCGAG TGTCAGGAAA CAGTAACGTA ACGCCGTATG ATTGGCTGAA GACAGAGCTT
    CCTCATGTGC TTGATGAGAT CTGTGTGATG GCTGCATCGT GTCAGCAGGA GCCCAACGGT
    TCAGGGGGGA ACAATGGCCA CGATGGACTA AAGGGCAACC CTGTGCCCCT GTCCAGAGCG
    GAAGCTAAAC GTGCCTGGCT GGCAGCAGGG GGCGATAATG AAAAGGCCGT CAGACAGGCT
    CTCAGAGACC GCAGAGTTAA AGTAAAAGAG CTTGGCTCTT TGGGCTTTAA TGATGCAACC
    CGCTGTGAAG AAGCACTGAG GCAGAGTGGA GGCGATGTAA GGGGGGCTCT AGTCCTGCTG
    CAGCGCCCTT TGCTGGAGCC CTTCCATCAG CGCATGTGGA GCGAAGAACC AGAGCCCCCC
    ATCGACATCA ACCACCCAGA TAAACAGCAT GTTTGCCGTA GGCTGCTAGC AGTGTTTGAT
    CTGCCCAGCT GGGGGCGCTG TGAGCTGATT TTGTCTTTGC TACGGGACCC TGCAGCCAAT
    TACACACTGG AAGACGTGGT TCAGGCTGTG CGTGAATCAC CTGACCGAGA CTTCATTCGG
    CGCGTGCTGA ACAAAGAATG CCCCATCTGC CTGTCAGTTT TTCCCCACAG CAAGATGCGG
    TCTCTGACAT CATGCCAGTG CTCAGTGTGC TGTGGATGTT TCCAGCAGCA TTTTACCATA
    GCGGTGAGAG ACAAACACAT CAGAGACATG GTGTGTCCTG TCTGCACAGA GCCTGACATC
    AACGACCCAG AGCACCTTAA CAGCTACTTC TCTACACTGG ACATACAGCT CAGGGATTGT
    CTTGAACAAG AGGTGTACGA GCTCTTCCAC AAGAAGCTAA CTGAGCAGGC TCTCATCAAG
    GACCCAAAGT TTCTGTGGTG CAGTCATTGC TCATATGGCT TCATCTATGA CGACGATCAA
    CTCAAGGTCA CCTGTAGCCA GTGCAGGAAA AGCTTTTGTG CTCAGTGCAA AAAAACATGG
    GAGCCACAGC ACATGGGTCT TTCGTGTGAG CAATTCCAGC TATGGAAGCG AGAGAATGAC
    CCTGAATACC AGAGGCAGGG TTTGGCCGGA TACCTGCGTG ACAACGGCAT CACTTGTCCT
    AACTGTAGGT TCCAGTATGC TCTGGCCAGG GGCGGCTGCA TGCACTTCAG CTGTTCTCAG
    TGCAGATATC AGTTCTGCAG TGGATGTAAC AACCCCTTCC ACACAACATG TGCAGTGAAC
    CAGTGTACTG TGACAGGTCT GCATGCCCAT CACCCCAGAG ACTGCCTCTT CTACCTCCGA
    GACTGGGAGC CTGCAAGGCT TCAGGCCTTA CTGCAGAAAA AAGGGGTGAA CTACAATACT
    GAAACTGCAC CAGGAGCACA GACTGGTGTG TGTGGAGTGA TCGAGCAGAA AGATGAAGGG
    CCTCGTCCGA CCGATTCTCC GTGTGGTGCT CAGACTCAGC CGGGCCAGGC GGGACTCTGC
    GATAAGCATT ATCGAGAGTA CCTGGTCAGC CTCATAAATG GCCATTCAAT TGATCCGGCT
    CTTCTTTTCA CAATGGACGA GCTAACAGTA GCCTGCAGGA GATACCATGT GGACATCACA
    CTGCTGGATA GAGAAGATGA ACAAACACAC TTTAGCAGAC TGCTTAAGAA ACTCATGGAT
    GACGTGCCTC TGGGTGACAA GGTCCTTCGC AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296840.2
    CDS21..3179
    Translation

    Target ORF information:

    RefSeq Version XM_009298565.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio E3 ubiquitin-protein ligase RNF31 (LOC100334178), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298565.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCTCTC TCACTGATCA GCTGGAGGAG GTGAGGGTGA ATGCGGAGGG CAGTTTATCT 
    TCTCCAAGTT CTGCCCAGGA GATGAGGGCA GGGGTGGAAG CTATGGCCAA CATCCCCCTT
    CCACCCTCCA GCAAGTATCG CTGTATAGCT GCCGAAGTCA TGTTGACAGA GAACGGTAGT
    GGCAATAACA GGAAGGAGTC CCTAGCCCTT CTGCAGAAAC TTTCTACTGC TCTTAACATT
    TTGGAGAAGT ATGGCTCCAA TCTTACCAAC CCCAGCAGAC CTAAGTACTG GCGAACAGTA
    AAGTACAACA ATCCTGTCTT CAGGACCACC GTAGACTCTA TTCAGGGTGG CAGAGCAGTA
    CTTAACCTGT ACGGCTACAC CAATCAGCAG CAGGATGGCT TAAGCTTTCC TGATGATGTT
    GTTGAGCCAG ATGTTGGCAA GGTGGCGTCA GTAACTCTTG AGGTCATGTG TCTTCGAATG
    GAGCTGGACA TGCTAACCAA GGGCACCCAT CCTCATCCAG AGTTTTTTGA AAGACTTATT
    CCCTCTCTCA CCGTGCAGGA GGAAGAGGGA CTCAGCACAG ATGCAGTTGT CTTTGCTCCT
    AGTGAGAAAG TATGGGATAA ACAAACTCCT TCACCTCTGT CTAGTCCTCC TCAGGCTAAA
    TCCCCTTTCA GTCCAGCTCT CTCTCTCTCT TCTCTGGCCC CCTCTACACA AACAGAGAAC
    TGCACCATAT GTGGCATCTT TCCAGTGTCC GCTCACTGTC CCACTTGTAC CCAGAAGCTT
    TGCACAGAAT GTGACAGACT CTATCACTCT CATTCTGGAC GATCTACACA CATAAGAATC
    CCTGTGACTT CCTCAAGACC CACAAAGCGA CTCAGCTCAT CACTGTCAAC ATGGCAGTGT
    TCATACTGCA CTACAGTAAA TTCGATGCTG CAGGTTTTGT GTGAAACCTG CGATCGCCCC
    CGTTTGTCCT CTGCTGCTCC ATCACTACAG GAGGAGTCAT CACAGCCCTC TACAATATCT
    GAGTGGCAGT GTAAAAGCTG CACAGTGGTG AATGCAGGTA GCAGTATTTT ATGTGAGGTT
    TGCGAACGAC CTCGTCTGGC CACCAGACCA CCTGCTGTAC CCCCACGAGC AACCCCTTCC
    TCCTCAGCAC TAATGGACAG CCAGTGGATA TGCCAGTTTT GTACATACGT CAACCGGACA
    CCATCGACCG TATGTGACAT GTGTTGTCTA TCAAGACCAG AGCCTTCCAT ATCACCCTCT
    AAGCCCCTCC CACTATCACC AGTCAAGGAC CTTCTACCTC CTGCAGCCAC AACCAAAGCC
    ACGCCCACTG AAGACCCTGA TTTAAAGCGA CAGAGACTGA TGAAGGAGGA CGGGCTCAAA
    CTTATTCAGC TCATTCGAGA GGGAGAGAAG AAAGGAGTGA GCCCAGAGGA GGTTTACACC
    AGCATGCGAG TGTCAGGAAA CAGTAACGTA ACGCCGTATG ATTGGCTGAA GACAGAGCTT
    CCTCATGTGC TTGATGAGAT CTGTGTGATG GCTGCATCGT GTCAGCAGGA GCCCAACGGT
    TCAGGGGGGA ACAATGGCCA CGATGGACTA AAGGGCAACC CTGTGCCCCT GTCCAGAGCG
    GAAGCTAAAC GTGCCTGGCT GGCAGCAGGG GGCGATAATG AAAAGGCCGT CAGACAGGCT
    CTCAGAGACC GCAGAGTTAA AGTAAAAGAG CTTGGCTCTT TGGGCTTTAA TGATGCAACC
    CGCTGTGAAG AAGCACTGAG GCAGAGTGGA GGCGATGTAA GGGGGGCTCT AGTCCTGCTG
    CAGCGCCCTT TGCTGGAGCC CTTCCATCAG CGCATGTGGA GCGAAGAACC AGAGCCCCCC
    ATCGACATCA ACCACCCAGA TAAACAGCAT GTTTGCCGTA GGCTGCTAGC AGTGTTTGAT
    CTGCCCAGCT GGGGGCGCTG TGAGCTGATT TTGTCTTTGC TACGGGACCC TGCAGCCAAT
    TACACACTGG AAGACGTGGT TCAGGCTGTG CGTGAATCAC CTGACCGAGA CTTCATTCGG
    CGCGTGCTGA ACAAAGAATG CCCCATCTGC CTGTCAGTTT TTCCCCACAG CAAGATGCGG
    TCTCTGACAT CATGCCAGTG CTCAGTGTGC TGTGGATGTT TCCAGCAGCA TTTTACCATA
    GCGGTGAGAG ACAAACACAT CAGAGACATG GTGTGTCCTG TCTGCACAGA GCCTGACATC
    AACGACCCAG AGCACCTTAA CAGCTACTTC TCTACACTGG ACATACAGCT CAGGGATTGT
    CTTGAACAAG AGGTGTACGA GCTCTTCCAC AAGAAGCTAA CTGAGCAGGC TCTCATCAAG
    GACCCAAAGT TTCTGTGGTG CAGTCATTGC TCATATGGCT TCATCTATGA CGACGATCAA
    CTCAAGGTCA CCTGTAGCCA GTGCAGGAAA AGCTTTTGTG CTCAGTGCAA AAAAACATGG
    GAGCCACAGC ACATGGGTCT TTCGTGTGAG CAATTCCAGC TATGGAAGCG AGAGAATGAC
    CCTGAATACC AGAGGCAGGG TTTGGCCGGA TACCTGCGTG ACAACGGCAT CACTTGTCCT
    AACTGTAGGT TCCAGTATGC TCTGGCCAGG GGCGGCTGCA TGCACTTCAG CTGTTCTCAG
    TGCAGATATC AGTTCTGCAG TGGATGTAAC AACCCCTTCC ACACAACATG TGCAGTGAAC
    CAGTGTACTG TGACAGGTCT GCATGCCCAT CACCCCAGAG ACTGCCTCTT CTACCTCCGA
    GACTGGGAGC CTGCAAGGCT TCAGGCCTTA CTGCAGAAAA AAGGGGTGAA CTACAATACT
    GAAACTGCAC CAGGAGCACA GACTGGTGTG TGTGGAGTGA TCGAGCAGAA AGATGAAGGG
    CCTCGTCCGA CCGATTCTCC GTGTGGTGCT CAGACTCAGC CGGGCCAGGC GGGACTCTGC
    GATAAGCATT ATCGAGAGTA CCTGGTCAGC CTCATAAATG GCCATTCAAT TGATCCGGCT
    CTTCTTTTCA CAATGGACGA GCTAACAGTA GCCTGCAGGA GATACCATGT GGACATCACA
    CTGCTGGATA GAGAAGATGA ACAAACACAC TTTAGCAGAC TGCTTAAGAA ACTCATGGAT
    GACGTGCCTC TGGGTGACAA GGTCCTTCGC AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.