clcn2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clcn2b
Entrez Gene ID	100333644
Full Name	chloride channel, voltage-sensitive 2b
General protein information	Preferred Names chloride channel, voltage-sensitive 2b Names chloride channel, voltage-sensitive 2b chloride channel 2b clc-2b chloride channel
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304042.2	XP_009302317.1	chloride channel, voltage-sensitive 2b isoform X1
XM_009304044.2	XP_009302319.1	chloride channel, voltage-sensitive 2b isoform X2
NM_001316315.1	NP_001303244.1	chloride channel, voltage-sensitive 2b
XM_009304047.3	XP_009302322.1	chloride channel, voltage-sensitive 2b isoform X3
XM_009304044.3	XP_009302319.1	chloride channel, voltage-sensitive 2b isoform X2
XM_009304047.2	XP_009302322.1	chloride channel, voltage-sensitive 2b isoform X3
XM_009304042.3	XP_009302317.1	chloride channel, voltage-sensitive 2b isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	clh-4	NP_001024885.1
Pan troglodytes (chimpanzee)	CLCN2	XP_003950294.1
Rattus norvegicus (Norway rat)	LOC100912455	XP_003751129.1
Caenorhabditis elegans (roundworm)	clh-3	NP_001022060.1
Canis lupus familiaris (dog)	CLCN2	XP_005639832.1
Bos taurus (cattle)	CLCN2	NP_001095957.1
Rattus norvegicus (Norway rat)	Clcn2	NP_058833.1
Xenopus tropicalis (tropical clawed frog)	clcn2	XP_004914466.1
	CLCN2	XP_002808378.1
Mus musculus (house mouse)	Clcn2	NP_034030.2
Danio rerio (zebrafish)	LOC100333644	XP_002667721.3
Drosophila melanogaster (fruit fly)	ClC-a	NP_001097752.1
Homo sapiens (human)	CLCN2	NP_004357.3
Gallus gallus (chicken)	CLCN2	XP_423073.4
Anopheles gambiae (African malaria mosquito)	GPRMGL4	XP_003436287.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Identification and characterization of the zebrafish ClC-2 chloride channel orthologs.
P?rez-Rius C, Gait?n-Pe?as H, Est?vez R, Barrallo-Gimeno A
Pflugers Archiv : European journal of physiology467(8)1769-81(2015 Aug)

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
Wang YF, Yan JJ, Tseng YC, Chen RD, Hwang PP
International journal of biological sciences11(10)1190-203(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification and characterization of the zebrafish ClC-2a/b/c chloride channel orthologs.
Title: Identification and characterization of the zebrafish ClC-2 chloride channel orthologs.

The following clcn2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clcn2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49833	NM_001316315.1 Latest version!	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49832	XM_009304042.3 Latest version!	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49833	XM_009304044.3 Latest version!	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49834	XM_009304047.3 Latest version!	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa49833	XM_009304044.2	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49834	XM_009304047.2	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49832	XM_009304042.2	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clcn2b ( NM_001316315.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001303244.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), mRNA.

Price & Availability↑

CloneID	ODa49833
Clone ID Related Accession (Same CDS sequence)	XM_009304044.2 , NM_001316315.1 , XM_009304044.3
Accession Version	NM_001316315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	chloride channel, voltage-sensitive 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from LN610102.1. ##Evidence-Data-START## Transcript exon combination :: LN610102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303244.1
CDS	1..2697
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRICAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEESNNVELRQSKRVRISLLDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	NM_001316315.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316315.1

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304042.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302317.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49832
Clone ID Related Accession (Same CDS sequence)	XM_009304042.2 , XM_009304042.3
Accession Version	XM_009304042.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGAGTAATA ACGTGGAGCT CAGACAGAGC 
AAACGCGTGC GGATCTCACT GTTGGATGAT CCAGACGCAG AGGATGACAT GACTCTTGGA 
GAGATTGAAG AGTGGGAGGA GCAACAGCTT GACGAGCAGG TGAACTTCAA CAGCTGCAAG 
ATTGACCCCG CCCCCTTCCA GCTGGTTGAG CGAACATCGC TACACAAGAC TCACACCATC 
TTCTCTCTGC TGGGTTTGGA TCATGCATAC GTCACCAGCA TCGGGAGACT CATTGGAGTG 
GTTTCATTGA GAGAGCTGCG TAAAGCCATC GAGGGCTCCA TGACGGTGAA GGGAGTGAAG 
GTTCGTCCTC CGCTGGCCAG TTTCAGAGAC AGCGGAACCA GCGGCACCAG CAGCGAGAGC 
GAAGCCACAG AACTGCACAA ACTGTGGGAC AGACACTCCA GCCTGAGCCT GCCCCGAGAC 
CAGCAGAACC ACAACACTGA CAACAAGACA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302317.1
CDS	163..2898
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRRFAYASAPPDFFSVCAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEESNNVELRQSKRVRISLLDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304042.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304042.2

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGAGTAATA ACGTGGAGCT CAGACAGAGC 
AAACGCGTGC GGATCTCACT GTTGGATGAT CCAGACGCAG AGGATGACAT GACTCTTGGA 
GAGATTGAAG AGTGGGAGGA GCAACAGCTT GACGAGCAGG TGAACTTCAA CAGCTGCAAG 
ATTGACCCCG CCCCCTTCCA GCTGGTTGAG CGAACATCGC TACACAAGAC TCACACCATC 
TTCTCTCTGC TGGGTTTGGA TCATGCATAC GTCACCAGCA TCGGGAGACT CATTGGAGTG 
GTTTCATTGA GAGAGCTGCG TAAAGCCATC GAGGGCTCCA TGACGGTGAA GGGAGTGAAG 
GTTCGTCCTC CGCTGGCCAG TTTCAGAGAC AGCGGAACCA GCGGCACCAG CAGCGAGAGC 
GAAGCCACAG AACTGCACAA ACTGTGGGAC AGACACTCCA GCCTGAGCCT GCCCCGAGAC 
CAGCAGAACC ACAACACTGA CAACAAGACA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304042.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302317.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49832
Clone ID Related Accession (Same CDS sequence)	XM_009304042.2 , XM_009304042.3
Accession Version	XM_009304042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGAGTAATA ACGTGGAGCT CAGACAGAGC 
AAACGCGTGC GGATCTCACT GTTGGATGAT CCAGACGCAG AGGATGACAT GACTCTTGGA 
GAGATTGAAG AGTGGGAGGA GCAACAGCTT GACGAGCAGG TGAACTTCAA CAGCTGCAAG 
ATTGACCCCG CCCCCTTCCA GCTGGTTGAG CGAACATCGC TACACAAGAC TCACACCATC 
TTCTCTCTGC TGGGTTTGGA TCATGCATAC GTCACCAGCA TCGGGAGACT CATTGGAGTG 
GTTTCATTGA GAGAGCTGCG TAAAGCCATC GAGGGCTCCA TGACGGTGAA GGGAGTGAAG 
GTTCGTCCTC CGCTGGCCAG TTTCAGAGAC AGCGGAACCA GCGGCACCAG CAGCGAGAGC 
GAAGCCACAG AACTGCACAA ACTGTGGGAC AGACACTCCA GCCTGAGCCT GCCCCGAGAC 
CAGCAGAACC ACAACACTGA CAACAAGACA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302317.1
CDS	164..2899
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRRFAYASAPPDFFSVCAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEESNNVELRQSKRVRISLLDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304042.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304042.3

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGAGTAATA ACGTGGAGCT CAGACAGAGC 
AAACGCGTGC GGATCTCACT GTTGGATGAT CCAGACGCAG AGGATGACAT GACTCTTGGA 
GAGATTGAAG AGTGGGAGGA GCAACAGCTT GACGAGCAGG TGAACTTCAA CAGCTGCAAG 
ATTGACCCCG CCCCCTTCCA GCTGGTTGAG CGAACATCGC TACACAAGAC TCACACCATC 
TTCTCTCTGC TGGGTTTGGA TCATGCATAC GTCACCAGCA TCGGGAGACT CATTGGAGTG 
GTTTCATTGA GAGAGCTGCG TAAAGCCATC GAGGGCTCCA TGACGGTGAA GGGAGTGAAG 
GTTCGTCCTC CGCTGGCCAG TTTCAGAGAC AGCGGAACCA GCGGCACCAG CAGCGAGAGC 
GAAGCCACAG AACTGCACAA ACTGTGGGAC AGACACTCCA GCCTGAGCCT GCCCCGAGAC 
CAGCAGAACC ACAACACTGA CAACAAGACA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304044.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302319.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49833
Clone ID Related Accession (Same CDS sequence)	XM_009304044.2 , NM_001316315.1 , XM_009304044.3
Accession Version	XM_009304044.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302319.1
CDS	163..2859
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRICAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEESNNVELRQSKRVRISLLDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304044.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304044.2

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304044.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302319.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49833
Clone ID Related Accession (Same CDS sequence)	XM_009304044.2 , NM_001316315.1 , XM_009304044.3
Accession Version	XM_009304044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302319.1
CDS	164..2860
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRICAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEESNNVELRQSKRVRISLLDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304044.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304044.3

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAATCTGT GCAGTGCACT GTCAGCGGTT CCTCATGTCG 
CGGGTCGGCG AGGACTGGAT CTTCCTCATT TTACTGGGAC TGGTCATGGC TCTGGTCAGC 
TTCGTCATGG ACTTCTGCAT CGCACTCCTC CTGCAGGCAC AGAAATGGAT GTACGGTGGC 
CTGGACAGTA ATGTGGTGCT GCAGTATCTG GCGTGGGTCA CTTATCCTGT GGTGCTCATC 
AGTTTCTCTG CCGGTTTCAC ACACATTGTC GCTCCACAGG CAGCCGGCTC TGGTATTCCC 
GAGATGAAGA CGATCCTCAG AGGTGTGGTG CTGAAGGAAT ATCTCACACT CAAGACCTTC 
GTGGCCAAAG TGGTCGGCCT GACCTGTGCG CTGGGTAGCG GCCTTCCTCT GGGGAAAGAG 
GGTCCGTTTG TGCACATCGC CAGTCTGTGT GCGGCTCTCC TCTGCAAGTT CATGTCTTTC 
TTTGGTGGAA TTTATGAGAA TGAGTCCAGG AACATTGAGA TGTTGGCTGC GGCCTGTGCT 
GTCGGCGTCG GCTGCTGCTT TGCTGCTCCC ATTGGAGGTG TGTTGTTCAG CATTGAGGTC 
ACCTCCACGT TTTTCGCCGT GAGGAATTAC TGGCGTGGAT TCTTTGCCGC CACATTCAGT 
GCCTTCATCT TCAGAGTCCT GGCCGTCTGG AACAGAGATG AAGAAACCAT CACTGCTCTC 
TTCAAGACGC GCTTCAGGCT TGATTTCCCC TTCGACCTGC AGGAGCTTCC AGCATTCGCC 
GTCATCGGGA TCGCCAGTGG GTTTGGTGGA GCTCTGTTTG TGTATCTGAA CAGGCTGATC 
GTTCAGTTCA TGCGCAAACA GAAAACCATT AACAAGTTCC TCATGAAGAA GCGTCTGCTA 
TATCCTGCTC TCGTTACTCT ACTGATATCC ACACTCTCCT TTCCACCAGG CTTTGGTCAA 
TTCATGGCAG GACAGCTCAC ACAGAAGGAG ACGCTCGTTT CGTTCTTCGA CAACCGTACC 
TGGGCCAAGC AGGGCATCTC AGAGGACTTC ACCTTCGACA CACACCTGAT GGCCTGGAAA 
CACCCACAGG CCAACGTCTT CATCATCCTC GTCATCTTTA TCATCATGAA GTTCTGGATG 
TCTGCTTTAG CCACAACGCT GCCGGTGCCC TGCGGAGCCT TCATGCCGGT TTTCGTCATT 
GGTGCTGCGT TTGGTCGTCT GGTGGGTGAA AGTATGGCTG CCTGGTTTCC AGAGGGCATC 
AACACAGACG GCACCATCTA TCCCATAGTT CCCGGTGGAT ACGCTGTTGT GGGAGCTGCA 
GCGCTGTCCG GAGCCGTCAC ACACACTGTC TCTACAGCAG TGATCGTGTT TGAGCTGACG 
GGTCAGATCA GTCACATTCT GCCCATCATG ATCGCTGTGA TTTTAGCCAA CGCTGTGGCT 
CAGAGTCTGC AGCCGTCCAT CTATGACTCC ATCATCCGCA TCAAGAAGCT GCCATACCTG 
CCGGAGCTGG GCTGGGGACA CCATGAGAAG TATAATATCC GCGTGGAGGA CATCATGGTG 
CGAGATGTTC GTTATATCAC ACTGAACTCC ACATACAGAG ACCTGCAAGA CATCCTCACG 
ATCGGAAACC TCAAAACTGT GGCGCTGGTG GAGTCTGCAG ATTCGATGAT TCTGCTGGGC 
TCTATTGAGC GCGCTCAGTT GCAGGCTCTC CTGGTTCAGC AGGTGAGCCG GGTGCGGCGA 
CAGGAAGTGA TGCGTGAGCG GGCGGAAACA GAGAGGAAGC GTATGTCGGT GGCCAGTTCA 
CCCAGCAGTG ATGACAGCAG TCAGAAAGTC AACCAGGAGG TTCGCTTCCA GATCTCCACA 
GAAGAGTCTA CGTTCGTTCC AGTTCGCCCG ATCTCTCCCA AACCTCTGAA ACCTGCGCTG 
AAGAGGACAT CTGTTGCTGA GAAAAACATC GAGATCCCAA CCAGTCCTCA TGACTCCAGC 
ATGGGTCTGA AGAATCTGCT GTGTGCTCGT CCTCAGACAG ACGTGATGGA GGAGAGTAAT 
AACGTGGAGC TCAGACAGAG CAAACGCGTG CGGATCTCAC TGTTGGATGA TCCAGACGCA 
GAGGATGACA TGACTCTTGG AGAGATTGAA GAGTGGGAGG AGCAACAGCT TGACGAGCAG 
GTGAACTTCA ACAGCTGCAA GATTGACCCC GCCCCCTTCC AGCTGGTTGA GCGAACATCG 
CTACACAAGA CTCACACCAT CTTCTCTCTG CTGGGTTTGG ATCATGCATA CGTCACCAGC 
ATCGGGAGAC TCATTGGAGT GGTTTCATTG AGAGAGCTGC GTAAAGCCAT CGAGGGCTCC 
ATGACGGTGA AGGGAGTGAA GGTTCGTCCT CCGCTGGCCA GTTTCAGAGA CAGCGGAACC 
AGCGGCACCA GCAGCGAGAG CGAAGCCACA GAACTGCACA AACTGTGGGA CAGACACTCC 
AGCCTGAGCC TGCCCCGAGA CCAGCAGAAC CACAACACTG ACAACAAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304047.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302322.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa49834
Clone ID Related Accession (Same CDS sequence)	XM_009304047.3 , XM_009304047.2
Accession Version	XM_009304047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGGATGATC CAGACGCAGA GGATGACATG 
ACTCTTGGAG AGATTGAAGA GTGGGAGGAG CAACAGCTTG ACGAGCAGGT GAACTTCAAC 
AGCTGCAAGA TTGACCCCGC CCCCTTCCAG CTGGTTGAGC GAACATCGCT ACACAAGACT 
CACACCATCT TCTCTCTGCT GGGTTTGGAT CATGCATACG TCACCAGCAT CGGGAGACTC 
ATTGGAGTGG TTTCATTGAG AGAGCTGCGT AAAGCCATCG AGGGCTCCAT GACGGTGAAG 
GGAGTGAAGG TTCGTCCTCC GCTGGCCAGT TTCAGAGACA GCGGAACCAG CGGCACCAGC 
AGCGAGAGCG AAGCCACAGA ACTGCACAAA CTGTGGGACA GACACTCCAG CCTGAGCCTG 
CCCCGAGACC AGCAGAACCA CAACACTGAC AACAAGACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302322.1
CDS	164..2848
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRRFAYASAPPDFFSVCAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEEDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304047.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304047.3

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGGATGATC CAGACGCAGA GGATGACATG 
ACTCTTGGAG AGATTGAAGA GTGGGAGGAG CAACAGCTTG ACGAGCAGGT GAACTTCAAC 
AGCTGCAAGA TTGACCCCGC CCCCTTCCAG CTGGTTGAGC GAACATCGCT ACACAAGACT 
CACACCATCT TCTCTCTGCT GGGTTTGGAT CATGCATACG TCACCAGCAT CGGGAGACTC 
ATTGGAGTGG TTTCATTGAG AGAGCTGCGT AAAGCCATCG AGGGCTCCAT GACGGTGAAG 
GGAGTGAAGG TTCGTCCTCC GCTGGCCAGT TTCAGAGACA GCGGAACCAG CGGCACCAGC 
AGCGAGAGCG AAGCCACAGA ACTGCACAAA CTGTGGGACA GACACTCCAG CCTGAGCCTG 
CCCCGAGACC AGCAGAACCA CAACACTGAC AACAAGACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcn2b ( XM_009304047.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302322.1 Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.

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CloneID	ODa49834
Clone ID Related Accession (Same CDS sequence)	XM_009304047.3 , XM_009304047.2
Accession Version	XM_009304047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chloride channel, voltage-sensitive 2b isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGGATGATC CAGACGCAGA GGATGACATG 
ACTCTTGGAG AGATTGAAGA GTGGGAGGAG CAACAGCTTG ACGAGCAGGT GAACTTCAAC 
AGCTGCAAGA TTGACCCCGC CCCCTTCCAG CTGGTTGAGC GAACATCGCT ACACAAGACT 
CACACCATCT TCTCTCTGCT GGGTTTGGAT CATGCATACG TCACCAGCAT CGGGAGACTC 
ATTGGAGTGG TTTCATTGAG AGAGCTGCGT AAAGCCATCG AGGGCTCCAT GACGGTGAAG 
GGAGTGAAGG TTCGTCCTCC GCTGGCCAGT TTCAGAGACA GCGGAACCAG CGGCACCAGC 
AGCGAGAGCG AAGCCACAGA ACTGCACAAA CTGTGGGACA GACACTCCAG CCTGAGCCTG 
CCCCGAGACC AGCAGAACCA CAACACTGAC AACAAGACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302322.1
CDS	163..2847
Translation	MAVDGQEQRALQYEQTLMYGRYTQELGVYAREEAARLREDGRQRRSISERSPSLKLLEYEKGRCVKCRRFAYASAPPDFFSVCAVHCQRFLMSRVGEDWIFLILLGLVMALVSFVMDFCIALLLQAQKWMYGGLDSNVVLQYLAWVTYPVVLISFSAGFTHIVAPQAAGSGIPEMKTILRGVVLKEYLTLKTFVAKVVGLTCALGSGLPLGKEGPFVHIASLCAALLCKFMSFFGGIYENESRNIEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRLIVQFMRKQKTINKFLMKKRLLYPALVTLLISTLSFPPGFGQFMAGQLTQKETLVSFFDNRTWAKQGISEDFTFDTHLMAWKHPQANVFIILVIFIIMKFWMSALATTLPVPCGAFMPVFVIGAAFGRLVGESMAAWFPEGINTDGTIYPIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPIMIAVILANAVAQSLQPSIYDSIIRIKKLPYLPELGWGHHEKYNIRVEDIMVRDVRYITLNSTYRDLQDILTIGNLKTVALVESADSMILLGSIERAQLQALLVQQVSRVRRQEVMRERAETERKRMSVASSPSSDDSSQKVNQEVRFQISTEESTFVPVRPISPKPLKPALKRTSVAEKNIEIPTSPHDSSMGLKNLLCARPQTDVMEEDDPDAEDDMTLGEIEEWEEQQLDEQVNFNSCKIDPAPFQLVERTSLHKTHTIFSLLGLDHAYVTSIGRLIGVVSLRELRKAIEGSMTVKGVKVRPPLASFRDSGTSGTSSESEATELHKLWDRHSSLSLPRDQQNHNTDNKTH

Target ORF information:

RefSeq Version	XM_009304047.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chloride channel, voltage-sensitive 2b (clcn2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304047.2

ORF Insert Sequence:

ATGGCTGTGG ACGGACAAGA GCAGCGCGCG CTCCAGTATG AGCAGACTCT GATGTATGGC 
CGCTACACTC AGGAACTGGG TGTATATGCT AGAGAAGAAG CAGCTCGTCT GCGTGAGGAT 
GGCCGACAGC GACGCTCCAT TTCTGAACGC AGTCCGTCGC TCAAACTGCT GGAGTACGAG 
AAGGGAAGAT GCGTCAAGTG TCGAAGGTTT GCATATGCAT CAGCGCCGCC GGACTTTTTT 
TCTGTCTGTG CAGTGCACTG TCAGCGGTTC CTCATGTCGC GGGTCGGCGA GGACTGGATC 
TTCCTCATTT TACTGGGACT GGTCATGGCT CTGGTCAGCT TCGTCATGGA CTTCTGCATC 
GCACTCCTCC TGCAGGCACA GAAATGGATG TACGGTGGCC TGGACAGTAA TGTGGTGCTG 
CAGTATCTGG CGTGGGTCAC TTATCCTGTG GTGCTCATCA GTTTCTCTGC CGGTTTCACA 
CACATTGTCG CTCCACAGGC AGCCGGCTCT GGTATTCCCG AGATGAAGAC GATCCTCAGA 
GGTGTGGTGC TGAAGGAATA TCTCACACTC AAGACCTTCG TGGCCAAAGT GGTCGGCCTG 
ACCTGTGCGC TGGGTAGCGG CCTTCCTCTG GGGAAAGAGG GTCCGTTTGT GCACATCGCC 
AGTCTGTGTG CGGCTCTCCT CTGCAAGTTC ATGTCTTTCT TTGGTGGAAT TTATGAGAAT 
GAGTCCAGGA ACATTGAGAT GTTGGCTGCG GCCTGTGCTG TCGGCGTCGG CTGCTGCTTT 
GCTGCTCCCA TTGGAGGTGT GTTGTTCAGC ATTGAGGTCA CCTCCACGTT TTTCGCCGTG 
AGGAATTACT GGCGTGGATT CTTTGCCGCC ACATTCAGTG CCTTCATCTT CAGAGTCCTG 
GCCGTCTGGA ACAGAGATGA AGAAACCATC ACTGCTCTCT TCAAGACGCG CTTCAGGCTT 
GATTTCCCCT TCGACCTGCA GGAGCTTCCA GCATTCGCCG TCATCGGGAT CGCCAGTGGG 
TTTGGTGGAG CTCTGTTTGT GTATCTGAAC AGGCTGATCG TTCAGTTCAT GCGCAAACAG 
AAAACCATTA ACAAGTTCCT CATGAAGAAG CGTCTGCTAT ATCCTGCTCT CGTTACTCTA 
CTGATATCCA CACTCTCCTT TCCACCAGGC TTTGGTCAAT TCATGGCAGG ACAGCTCACA 
CAGAAGGAGA CGCTCGTTTC GTTCTTCGAC AACCGTACCT GGGCCAAGCA GGGCATCTCA 
GAGGACTTCA CCTTCGACAC ACACCTGATG GCCTGGAAAC ACCCACAGGC CAACGTCTTC 
ATCATCCTCG TCATCTTTAT CATCATGAAG TTCTGGATGT CTGCTTTAGC CACAACGCTG 
CCGGTGCCCT GCGGAGCCTT CATGCCGGTT TTCGTCATTG GTGCTGCGTT TGGTCGTCTG 
GTGGGTGAAA GTATGGCTGC CTGGTTTCCA GAGGGCATCA ACACAGACGG CACCATCTAT 
CCCATAGTTC CCGGTGGATA CGCTGTTGTG GGAGCTGCAG CGCTGTCCGG AGCCGTCACA 
CACACTGTCT CTACAGCAGT GATCGTGTTT GAGCTGACGG GTCAGATCAG TCACATTCTG 
CCCATCATGA TCGCTGTGAT TTTAGCCAAC GCTGTGGCTC AGAGTCTGCA GCCGTCCATC 
TATGACTCCA TCATCCGCAT CAAGAAGCTG CCATACCTGC CGGAGCTGGG CTGGGGACAC 
CATGAGAAGT ATAATATCCG CGTGGAGGAC ATCATGGTGC GAGATGTTCG TTATATCACA 
CTGAACTCCA CATACAGAGA CCTGCAAGAC ATCCTCACGA TCGGAAACCT CAAAACTGTG 
GCGCTGGTGG AGTCTGCAGA TTCGATGATT CTGCTGGGCT CTATTGAGCG CGCTCAGTTG 
CAGGCTCTCC TGGTTCAGCA GGTGAGCCGG GTGCGGCGAC AGGAAGTGAT GCGTGAGCGG 
GCGGAAACAG AGAGGAAGCG TATGTCGGTG GCCAGTTCAC CCAGCAGTGA TGACAGCAGT 
CAGAAAGTCA ACCAGGAGGT TCGCTTCCAG ATCTCCACAG AAGAGTCTAC GTTCGTTCCA 
GTTCGCCCGA TCTCTCCCAA ACCTCTGAAA CCTGCGCTGA AGAGGACATC TGTTGCTGAG 
AAAAACATCG AGATCCCAAC CAGTCCTCAT GACTCCAGCA TGGGTCTGAA GAATCTGCTG 
TGTGCTCGTC CTCAGACAGA CGTGATGGAG GAGGATGATC CAGACGCAGA GGATGACATG 
ACTCTTGGAG AGATTGAAGA GTGGGAGGAG CAACAGCTTG ACGAGCAGGT GAACTTCAAC 
AGCTGCAAGA TTGACCCCGC CCCCTTCCAG CTGGTTGAGC GAACATCGCT ACACAAGACT 
CACACCATCT TCTCTCTGCT GGGTTTGGAT CATGCATACG TCACCAGCAT CGGGAGACTC 
ATTGGAGTGG TTTCATTGAG AGAGCTGCGT AAAGCCATCG AGGGCTCCAT GACGGTGAAG 
GGAGTGAAGG TTCGTCCTCC GCTGGCCAGT TTCAGAGACA GCGGAACCAG CGGCACCAGC 
AGCGAGAGCG AAGCCACAGA ACTGCACAAA CTGTGGGACA GACACTCCAG CCTGAGCCTG 
CCCCGAGACC AGCAGAACCA CAACACTGAC AACAAGACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Identification and characterization of the zebrafish ClC-2 chloride channel orthologs.
Pflugers Archiv : European journal of physiology467(8)1769-81(2015 Aug)
P?rez-Rius C,Gait?n-Pe?as H,Est?vez R,Barrallo-Gimeno A

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
International journal of biological sciences11(10)1190-203(2015)
Wang YF,Yan JJ,Tseng YC,Chen RD,Hwang PP

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clcn2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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