ORF » Species Summary » Danio rerio » SEMA4D cDNA ORF clone

SEMA4D cDNA ORF clone, Danio rerio(Zebrafish)

The following SEMA4D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA4D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa49808 XM_009301688.2
Latest version!		 Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa150970 XM_021468958.1
Latest version!		 Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SEMA4D ( XM_009301688.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299963.1 Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA.
    Price & Availability↑
    CloneID ODa49808
    Clone ID Related Accession (Same CDS sequence) XM_009301688.2
    Accession Version XM_009301688.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-4D
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) and transcript sequence (GDQQ01015375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 44 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATCAGT TTTCGCCTCT CGATGCCATG GCGTTCAAAG GGAACCTCAC GCTGCCAAAG 
    GTGCCGGAGT CCAACATGGC CCTCAGTGTG CTGGGTGTGT TTCTGGGTTT GCTTCTGGAA 
    GTTTCCACGC ACGGGCCGTC CAGCGTTCCC AGAAGCTCAT GGAAGCAGAA CGATGTGGAT 
    CTGCTGGAGT TTTGGGAGCC GGATGTCTTC AACTACACGA CTCTGCTGCT GAATGAGGAC 
    AGAGGTGTTC TGTATGTGGG CGCTCGAGAG GCTGTGTATG AACTCAACAT CAGCAACATC 
    TCCATCAAGA ACAACCAGCT CCTGTGGAAA GTTCCAGACA TTGACATGTC CATGTGTATT 
    TTAAAGGGGA AATCTAAAGA GACGGACTGT CGAAACTACA TTCGAGTTTT GCAAGTGCTG 
    GATGAAGAAC ATCTGTATGT TTGCGGGACT CACGCGTTTC AGCCTCTCTG TGATTATCTG 
    TCTCTGCAAG ATTTCCGGCT GCTCGAGAAA GCAGAGGACG GAAGAGGGAA ATGTTCCTAT 
    GATCCGTCTC AAAGCTTCAC CACTGTGATG GTTGATGGCG AGTTGTACTC AGGAACTGCT 
    TATAACTTTC TGGGCAGTGA ACCGATCATC TCCAGATCGT CTCCGTCTCA GAGTTTACTG 
    AGAACTGAGT ACTCAACATC CTGGCTCAAT GAGCCCAGTT TCGTCTTTGC GGACGTCATC 
    CGAGAAGGAC AGGACAGTCC TGATGGAGAC GATGACAAGA TTTACTACTT CTTCACGGAG 
    GTGTCGGTGG AGTACGAGTT TTTCGGCAAA CTGCTGATTC CCAGGATCGC TCGCGTCTGT 
    AAGGGGGATC TGGGTGGCCA GCGGACCCTG CAGAAGAAGT GGACGTCGTT TCTGAAGGCC 
    AAGCTGGTTT GCTCAATGCC GGAGCTGAAC TTTGTCTTCA ATGTGGTTCA TGATGTGTTT 
    ATTTTAAAAG CCCCACACTG GAGAGACACC GTCATCTACG GGGTTTTCAC TTCTCAGTGG 
    GGGAATGTAG GTCTGTCGGC GGTCTGTGCG TATAACATGA GCTCAGTGCA TGAGGTTTTC 
    TCCAGAGGAA AGTACATGCA GAAGGTCACG GTTGAACAGT CCCACACAAA GTGGGTCCGC 
    TTTAATGGAG CGCCGCCCAG TCCCAGACCT GGAGCGTGCA TCAATAACAT GAACCGTCAG 
    CAGAACATGA GCTCGTCTCT TCATCTGCCT GATAAGACGC TTCAGTTTGT GAGGGATCAT 
    CCGCTGCTGG AGGACCCGGT GCTGCCCATA GGAAACGGCC CTCGCCTCAT CACTAAAGAT 
    GTGAACTACA CACAGATCGC CGTGCAGAGG GTGCAAGCGC TCGACAACAG CATACACCAC 
    GTCATTTTCA CAGCCACCGA TAAAGGTTTC CTGCATAAGT CAGTGGTGTT GGAGAATGAG 
    GTTCACACGG TGGAGGAGGT TCAGCTGCTG AAGAACTCAG AGCCCATCAA AACACTCCTG 
    CTCTCCACAG ACCAGGCTCA GTTTCTGTAT GCGGGTTCAG ACTCAGGAGT GGTTCAGTCA 
    CCCACAGCGT TCTGTCAGAA ATACGGCTCC TGTGTGGACT GTGTTCTGGC CCGGGACCCA 
    TACTGCGCCT GGGACACTCA ATCCAAAGCC TGTGTGAACA TCTTCCAGAG CAAAGCTGAT 
    CCACGTAAGA TGATTCAGAA CCTCAAAGGG GATGCTGGGA TATGTCCTCC AGTGTCTCCT 
    CTGGCAGTGA AGGATTATCC GCTGCTGTTG GTGAAGCCGG GTGCTTCAGC TGACCTGCCG 
    TGTCGAGTTC AGTCAAACCG AGCTCAGGTC TTGTGGAAAC TCAACAATCA GCTTCTCAGC 
    GACTCCTCTT CACGCCTCCT CCTGATGTCC GAGAGCAGTT TACTGCTGTA CAGCGTGACT 
    ATGGAGGACG CGGGACGCTA CGAATGCTGG TCCGTCGAGT CCGCCGCGGG ACAAAACTTC 
    AGCCGTGTGG TGTCCGGATA CATTCTGCGC TTAGACCTTC CCGAGAGCAA ATCTGCACAG 
    CTGACCCGCG AGCCCACTAC GACCTCAGAA GGTAATAGTG TTAATAATAA TAACAGCCCG 
    GTGACGCCTG GACTAACGTC AGCGCCTACA GATGCCCCGT CACTAACAAC TCCACCAACC 
    TCTGCTATTA AACCCAAATC CAACATCCCT CCAGAGGTCA GTAACTCGCC CGCACGCACT 
    GAAACGATAG ATCCTTCTGC CAAATACATC CAGCATGATA ACAGCGCCGC CCTGCTGGCT 
    CTGTTCCTGC TGTTCTTCCT GCTGTTTCTG GCCGCGCTCC TGTATAACTG CTACATGCAG 
    TATCTGCCGG CTCCGTGTCT ACGGCTGCGC TCCGCTCTGC TCGGATCCAG TAAAAAACCG 
    CAGGCCGAAT ACGCTGCATG CGAAGCCGGT CTGATGGAGA AAACTGATCC AGCCAATCAG 
    ATCGCTGCTC ATCCGCTCCG TGCACTCAGA GACACTGGAT ATGAAACGGA GCCGGAGGCG 
    GAATTAGGAA ACGGCGCTGT TCGGTCTAAT AGTTTCGATG AGAGTCCGTG TAAAGATCGA 
    CCGTTTGATA TCGACTGTGC TTCACAGCCT ATTGAGTTTG CAGATGCAGA TGCAGACGCT 
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299963.1
    CDS471..3116
    Translation

    Target ORF information:

    RefSeq Version XM_009301688.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATCAGT TTTCGCCTCT CGATGCCATG GCGTTCAAAG GGAACCTCAC GCTGCCAAAG 
    GTGCCGGAGT CCAACATGGC CCTCAGTGTG CTGGGTGTGT TTCTGGGTTT GCTTCTGGAA 
    GTTTCCACGC ACGGGCCGTC CAGCGTTCCC AGAAGCTCAT GGAAGCAGAA CGATGTGGAT 
    CTGCTGGAGT TTTGGGAGCC GGATGTCTTC AACTACACGA CTCTGCTGCT GAATGAGGAC 
    AGAGGTGTTC TGTATGTGGG CGCTCGAGAG GCTGTGTATG AACTCAACAT CAGCAACATC 
    TCCATCAAGA ACAACCAGCT CCTGTGGAAA GTTCCAGACA TTGACATGTC CATGTGTATT 
    TTAAAGGGGA AATCTAAAGA GACGGACTGT CGAAACTACA TTCGAGTTTT GCAAGTGCTG 
    GATGAAGAAC ATCTGTATGT TTGCGGGACT CACGCGTTTC AGCCTCTCTG TGATTATCTG 
    TCTCTGCAAG ATTTCCGGCT GCTCGAGAAA GCAGAGGACG GAAGAGGGAA ATGTTCCTAT 
    GATCCGTCTC AAAGCTTCAC CACTGTGATG GTTGATGGCG AGTTGTACTC AGGAACTGCT 
    TATAACTTTC TGGGCAGTGA ACCGATCATC TCCAGATCGT CTCCGTCTCA GAGTTTACTG 
    AGAACTGAGT ACTCAACATC CTGGCTCAAT GAGCCCAGTT TCGTCTTTGC GGACGTCATC 
    CGAGAAGGAC AGGACAGTCC TGATGGAGAC GATGACAAGA TTTACTACTT CTTCACGGAG 
    GTGTCGGTGG AGTACGAGTT TTTCGGCAAA CTGCTGATTC CCAGGATCGC TCGCGTCTGT 
    AAGGGGGATC TGGGTGGCCA GCGGACCCTG CAGAAGAAGT GGACGTCGTT TCTGAAGGCC 
    AAGCTGGTTT GCTCAATGCC GGAGCTGAAC TTTGTCTTCA ATGTGGTTCA TGATGTGTTT 
    ATTTTAAAAG CCCCACACTG GAGAGACACC GTCATCTACG GGGTTTTCAC TTCTCAGTGG 
    GGGAATGTAG GTCTGTCGGC GGTCTGTGCG TATAACATGA GCTCAGTGCA TGAGGTTTTC 
    TCCAGAGGAA AGTACATGCA GAAGGTCACG GTTGAACAGT CCCACACAAA GTGGGTCCGC 
    TTTAATGGAG CGCCGCCCAG TCCCAGACCT GGAGCGTGCA TCAATAACAT GAACCGTCAG 
    CAGAACATGA GCTCGTCTCT TCATCTGCCT GATAAGACGC TTCAGTTTGT GAGGGATCAT 
    CCGCTGCTGG AGGACCCGGT GCTGCCCATA GGAAACGGCC CTCGCCTCAT CACTAAAGAT 
    GTGAACTACA CACAGATCGC CGTGCAGAGG GTGCAAGCGC TCGACAACAG CATACACCAC 
    GTCATTTTCA CAGCCACCGA TAAAGGTTTC CTGCATAAGT CAGTGGTGTT GGAGAATGAG 
    GTTCACACGG TGGAGGAGGT TCAGCTGCTG AAGAACTCAG AGCCCATCAA AACACTCCTG 
    CTCTCCACAG ACCAGGCTCA GTTTCTGTAT GCGGGTTCAG ACTCAGGAGT GGTTCAGTCA 
    CCCACAGCGT TCTGTCAGAA ATACGGCTCC TGTGTGGACT GTGTTCTGGC CCGGGACCCA 
    TACTGCGCCT GGGACACTCA ATCCAAAGCC TGTGTGAACA TCTTCCAGAG CAAAGCTGAT 
    CCACGTAAGA TGATTCAGAA CCTCAAAGGG GATGCTGGGA TATGTCCTCC AGTGTCTCCT 
    CTGGCAGTGA AGGATTATCC GCTGCTGTTG GTGAAGCCGG GTGCTTCAGC TGACCTGCCG 
    TGTCGAGTTC AGTCAAACCG AGCTCAGGTC TTGTGGAAAC TCAACAATCA GCTTCTCAGC 
    GACTCCTCTT CACGCCTCCT CCTGATGTCC GAGAGCAGTT TACTGCTGTA CAGCGTGACT 
    ATGGAGGACG CGGGACGCTA CGAATGCTGG TCCGTCGAGT CCGCCGCGGG ACAAAACTTC 
    AGCCGTGTGG TGTCCGGATA CATTCTGCGC TTAGACCTTC CCGAGAGCAA ATCTGCACAG 
    CTGACCCGCG AGCCCACTAC GACCTCAGAA GGTAATAGTG TTAATAATAA TAACAGCCCG 
    GTGACGCCTG GACTAACGTC AGCGCCTACA GATGCCCCGT CACTAACAAC TCCACCAACC 
    TCTGCTATTA AACCCAAATC CAACATCCCT CCAGAGGTCA GTAACTCGCC CGCACGCACT 
    GAAACGATAG ATCCTTCTGC CAAATACATC CAGCATGATA ACAGCGCCGC CCTGCTGGCT 
    CTGTTCCTGC TGTTCTTCCT GCTGTTTCTG GCCGCGCTCC TGTATAACTG CTACATGCAG 
    TATCTGCCGG CTCCGTGTCT ACGGCTGCGC TCCGCTCTGC TCGGATCCAG TAAAAAACCG 
    CAGGCCGAAT ACGCTGCATG CGAAGCCGGT CTGATGGAGA AAACTGATCC AGCCAATCAG 
    ATCGCTGCTC ATCCGCTCCG TGCACTCAGA GACACTGGAT ATGAAACGGA GCCGGAGGCG 
    GAATTAGGAA ACGGCGCTGT TCGGTCTAAT AGTTTCGATG AGAGTCCGTG TAAAGATCGA 
    CCGTTTGATA TCGACTGTGC TTCACAGCCT ATTGAGTTTG CAGATGCAGA TGCAGACGCT 
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SEMA4D ( XM_021468958.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324633.1 Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA.
    Price & Availability↑
    CloneID ODa150970
    Clone ID Related Accession (Same CDS sequence) XM_021468958.1
    Accession Version XM_021468958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-4D
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) and transcript sequence (GFIL01034772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1550 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCAGT TTTCGCCTCT CGATGCCATG GCGTTCAAAG GGAACCTCAC GCTGCCAAAG 
    GTGCCGGAGT CCAACATGGC CCTCAGTGTG CTGGGTGTGT TTCTGGGTTT GCTTCTGGAA 
    GTTTCCACGC ACGGGCCGTC CAGCGTTCCC AGAAGCTCAT GGAAGCAGAA CGATGTGGAT 
    CTGCTGGAGT TTTGGGAGCC GGATGTCTTC AACTACACGA CTCTGCTGCT GAATGAGGAC 
    AGAGGTGTTC TGTATGTGGG CGCTCGAGAG GCTGTGTATG AACTCAACAT CAGCAACATC 
    TCCATCAAGA ACAACCAGCT CCTGTGGAAA GTTCCAGACA TTGACATGTC CATGTGTATT 
    TTAAAGGGGA AATCTAAAGA GACGGACTGT CGAAACTACA TTCGAGTTTT GCAAGTGCTG 
    GATGAAGAAC ATCTGTATGT TTGCGGGACT CACGCGTTTC AGCCTCTCTG TGATTATCTG 
    TCTCTGCAAG ATTTCCGGCT GCTCGAGAAA GCAGAGGACG GAAGAGGGAA ATGTTCCTAT 
    GATCCGTCTC AAAGCTTCAC CACTGTGATG GTTGATGGCG AGTTGTACTC AGGAACTGCT 
    TATAACTTTC TGGGCAGTGA ACCGATCATC TCCAGATCGT CTCCGTCTCA GAGTTTACTG 
    AGAACTGAGT ACTCAACATC CTGGCTCAAT GAGCCCAGTT TCGTCTTTGC GGACGTCATC 
    CGAGAAGGAC AGGACAGTCC TGATGGAGAC GATGACAAGA TTTACTACTT CTTCACGGAG 
    GTGTCGGTGG AGTACGAGTT TTTCGGCAAA CTGCTGATTC CCAGGATCGC TCGCGTCTGT 
    AAGGGGGATC TGGGTGGCCA GCGGACCCTG CAGAAGAAGT GGACGTCGTT TCTGAAGGCC 
    AAGCTGGTTT GCTCAATGCC GGAGCTGAAC TTTGTCTTCA ATGTGGTTCA TGATGTGTTT 
    ATTTTAAAAG CCCCACACTG GAGAGACACC GTCATCTACG GGGTTTTCAC TTCTCAGTGG 
    GGGAATGTAG GTCTGTCGGC GGTCTGTGCG TATAACATGA GCTCAGTGCA TGAGGTTTTC 
    TCCAGAGGAA AGTACATGCA GAAGGTCACG GTTGAACAGT CCCACACAAA GTGGGTCCGC 
    TTTAATGGAG CGCCGCCCAG TCCCAGACCT GGAGCGTGCA TCAATAACAT GAACCGTCAG 
    CAGAACATGA GCTCGTCTCT TCATCTGCCT GATAAGACGC TTCAGTTTGT GAGGGATCAT 
    CCGCTGCTGG AGGACCCGGT GCTGCCCATA GGAAACGGCC CTCGCCTCAT CACTAAAGAT 
    GTGAACTACA CACAGATCGC CGTGCAGAGG GTGCAAGCGC TCGACAACAG CATACACCAC 
    GTCATTTTCA CAGCCACCGA TAAAGGTTTC CTGCATAAGT CAGTGGTGTT GGAGAATGAG 
    GTTCACACGG TGGAGGAGGT TCAGCTGCTG AAGAACTCAG AGCCCATCAA AACACTCCTG 
    CTCTCCACAG ACCAGGCTCA GTTTCTGTAT GCGGGTTCAG ACTCAGGAGT GGTTCAGTCA 
    CCCACAGCGT TCTGTCAGAA ATACGGCTCC TGTGTGGACT GTGTTCTGGC CCGGGACCCA 
    TACTGCGCCT GGGACACTCA ATCCAAAGCC TGTGTGAACA TCTTCCAGAG CAAAGCTGAT 
    CCACGTAAGA TGATTCAGAA CCTCAAAGGG GATGCTGGGA TATGTCCTCC AGTGTCTCCT 
    CTGGCAGTGA AGGATTATCC GCTGCTGTTG GTGAAGCCGG GTGCTTCAGC TGACCTGCCG 
    TGTCGAGTTC AGTCAAACCG AGCTCAGGTC TTGTGGAAAC TCAACAATCA GCTTCTCAGC 
    GACTCCTCTT CACGCCTCCT CCTGATGTCC GAGAGCAGTT TACTGCTGTA CAGCGTGACT 
    ATGGAGGACG CGGGACGCTA CGAATGCTGG TCCGTCGAGT CCGCCGCGGG ACAAAACTTC 
    AGCCGTGTGG TGTCCGGATA CATTCTGCGC TTAGACCTTC CCGAGAGCAA ATCTGCACAG 
    CTGACCCGCG AGCCCACTAC GACCTCAGAA GCTCCGCCTC TTCCTCTTCC TCCTCCTCTT 
    CCTCCGTCCG CTGCTGTGGA TGGGGGAGTG TCTCTGGTTC CTCCTCTGCT GAGTCAGCAG 
    TCGTGGACTC TTCACACTCA TCAGGCTTTC CTGCTCTTCT GTCTGGCTGT GGGTCCTGGA 
    GATGTGAGTT TTGAGTGGCG TGTGAACGGC CGGAGACTTG AGTCTGGCAT CACCGAACAT 
    AGACGTGTGT TTTCCCATGA TGCCGTTGTG CTGAGCAGCT GGATGAAGGC CGAGGCTCTG 
    AATGAAGATG TCCAGTATGA ATGTAAAGCT CTGTCCACAA CCGGGAACGA CACTTCCAGA 
    GTAACGCTAC GATCGTCCAG CAGAGATGAG GCTGATGTTT GTCACACAGA TGTGGAAGAC 
    TGGAGGACTG CAATGTCTGA ACACAACACA CTTCTGCAGC AGTGGAGGAA AGCCTGGGAA 
    GGCTGTGATG GACAAGGTGT TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324633.1
    CDS182..2728
    Translation

    Target ORF information:

    RefSeq Version XM_021468958.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (sema4d), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021468958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCAGT TTTCGCCTCT CGATGCCATG GCGTTCAAAG GGAACCTCAC GCTGCCAAAG 
    GTGCCGGAGT CCAACATGGC CCTCAGTGTG CTGGGTGTGT TTCTGGGTTT GCTTCTGGAA 
    GTTTCCACGC ACGGGCCGTC CAGCGTTCCC AGAAGCTCAT GGAAGCAGAA CGATGTGGAT 
    CTGCTGGAGT TTTGGGAGCC GGATGTCTTC AACTACACGA CTCTGCTGCT GAATGAGGAC 
    AGAGGTGTTC TGTATGTGGG CGCTCGAGAG GCTGTGTATG AACTCAACAT CAGCAACATC 
    TCCATCAAGA ACAACCAGCT CCTGTGGAAA GTTCCAGACA TTGACATGTC CATGTGTATT 
    TTAAAGGGGA AATCTAAAGA GACGGACTGT CGAAACTACA TTCGAGTTTT GCAAGTGCTG 
    GATGAAGAAC ATCTGTATGT TTGCGGGACT CACGCGTTTC AGCCTCTCTG TGATTATCTG 
    TCTCTGCAAG ATTTCCGGCT GCTCGAGAAA GCAGAGGACG GAAGAGGGAA ATGTTCCTAT 
    GATCCGTCTC AAAGCTTCAC CACTGTGATG GTTGATGGCG AGTTGTACTC AGGAACTGCT 
    TATAACTTTC TGGGCAGTGA ACCGATCATC TCCAGATCGT CTCCGTCTCA GAGTTTACTG 
    AGAACTGAGT ACTCAACATC CTGGCTCAAT GAGCCCAGTT TCGTCTTTGC GGACGTCATC 
    CGAGAAGGAC AGGACAGTCC TGATGGAGAC GATGACAAGA TTTACTACTT CTTCACGGAG 
    GTGTCGGTGG AGTACGAGTT TTTCGGCAAA CTGCTGATTC CCAGGATCGC TCGCGTCTGT 
    AAGGGGGATC TGGGTGGCCA GCGGACCCTG CAGAAGAAGT GGACGTCGTT TCTGAAGGCC 
    AAGCTGGTTT GCTCAATGCC GGAGCTGAAC TTTGTCTTCA ATGTGGTTCA TGATGTGTTT 
    ATTTTAAAAG CCCCACACTG GAGAGACACC GTCATCTACG GGGTTTTCAC TTCTCAGTGG 
    GGGAATGTAG GTCTGTCGGC GGTCTGTGCG TATAACATGA GCTCAGTGCA TGAGGTTTTC 
    TCCAGAGGAA AGTACATGCA GAAGGTCACG GTTGAACAGT CCCACACAAA GTGGGTCCGC 
    TTTAATGGAG CGCCGCCCAG TCCCAGACCT GGAGCGTGCA TCAATAACAT GAACCGTCAG 
    CAGAACATGA GCTCGTCTCT TCATCTGCCT GATAAGACGC TTCAGTTTGT GAGGGATCAT 
    CCGCTGCTGG AGGACCCGGT GCTGCCCATA GGAAACGGCC CTCGCCTCAT CACTAAAGAT 
    GTGAACTACA CACAGATCGC CGTGCAGAGG GTGCAAGCGC TCGACAACAG CATACACCAC 
    GTCATTTTCA CAGCCACCGA TAAAGGTTTC CTGCATAAGT CAGTGGTGTT GGAGAATGAG 
    GTTCACACGG TGGAGGAGGT TCAGCTGCTG AAGAACTCAG AGCCCATCAA AACACTCCTG 
    CTCTCCACAG ACCAGGCTCA GTTTCTGTAT GCGGGTTCAG ACTCAGGAGT GGTTCAGTCA 
    CCCACAGCGT TCTGTCAGAA ATACGGCTCC TGTGTGGACT GTGTTCTGGC CCGGGACCCA 
    TACTGCGCCT GGGACACTCA ATCCAAAGCC TGTGTGAACA TCTTCCAGAG CAAAGCTGAT 
    CCACGTAAGA TGATTCAGAA CCTCAAAGGG GATGCTGGGA TATGTCCTCC AGTGTCTCCT 
    CTGGCAGTGA AGGATTATCC GCTGCTGTTG GTGAAGCCGG GTGCTTCAGC TGACCTGCCG 
    TGTCGAGTTC AGTCAAACCG AGCTCAGGTC TTGTGGAAAC TCAACAATCA GCTTCTCAGC 
    GACTCCTCTT CACGCCTCCT CCTGATGTCC GAGAGCAGTT TACTGCTGTA CAGCGTGACT 
    ATGGAGGACG CGGGACGCTA CGAATGCTGG TCCGTCGAGT CCGCCGCGGG ACAAAACTTC 
    AGCCGTGTGG TGTCCGGATA CATTCTGCGC TTAGACCTTC CCGAGAGCAA ATCTGCACAG 
    CTGACCCGCG AGCCCACTAC GACCTCAGAA GCTCCGCCTC TTCCTCTTCC TCCTCCTCTT 
    CCTCCGTCCG CTGCTGTGGA TGGGGGAGTG TCTCTGGTTC CTCCTCTGCT GAGTCAGCAG 
    TCGTGGACTC TTCACACTCA TCAGGCTTTC CTGCTCTTCT GTCTGGCTGT GGGTCCTGGA 
    GATGTGAGTT TTGAGTGGCG TGTGAACGGC CGGAGACTTG AGTCTGGCAT CACCGAACAT 
    AGACGTGTGT TTTCCCATGA TGCCGTTGTG CTGAGCAGCT GGATGAAGGC CGAGGCTCTG 
    AATGAAGATG TCCAGTATGA ATGTAAAGCT CTGTCCACAA CCGGGAACGA CACTTCCAGA 
    GTAACGCTAC GATCGTCCAG CAGAGATGAG GCTGATGTTT GTCACACAGA TGTGGAAGAC 
    TGGAGGACTG CAATGTCTGA ACACAACACA CTTCTGCAGC AGTGGAGGAA AGCCTGGGAA 
    GGCTGTGATG GACAAGGTGT TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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