SI:CH73-212J7.1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH73-212J7.1
Entrez Gene ID	100318353
Full Name	si:ch73-212j7.1
General protein information	Preferred Names si:ch73-212j7.1 Names protein KIAA0284 centrosomal protein of 170 kDa protein B solute carrier organic anion transporter family member 2A1-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160328.4	XP_005160385.1	centrosomal protein of 170 kDa protein B isoform X1
XM_005160329.4	XP_005160386.1	centrosomal protein of 170 kDa protein B isoform X3
XM_005160329.3	XP_005160386.1	centrosomal protein of 170 kDa protein B isoform X3
XM_002665642.6	XP_002665688.3	centrosomal protein of 170 kDa protein B isoform X2
XM_005160328.3	XP_005160385.1	centrosomal protein of 170 kDa protein B isoform X1
XM_002665642.5	XP_002665688.3	centrosomal protein of 170 kDa protein B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KIAA0284	XP_003952654.1
Canis lupus familiaris (dog)	KIAA0284	XP_005624002.1
Bos taurus (cattle)	KIAA0284	XP_002696837.2
Rattus norvegicus (Norway rat)	Cep170b	XP_576105.4
	LOC697998	XP_001085960.2
Gallus gallus (chicken)	KIAA0284	XP_004936461.1
Danio rerio (zebrafish)	si:ch73-212j7.1	XP_005160385.1
Homo sapiens (human)	CEP170B	NP_001106197.1
Mus musculus (house mouse)	Cep170b	NP_001019773.2
Xenopus tropicalis (tropical clawed frog)	cep170b	NP_001072723.1

Gene Ontology

The following SI:CH73-212J7.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH73-212J7.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24607	XM_005160328.4 Latest version!	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa24608	XM_002665642.6 Latest version!	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa24609	XM_005160329.4 Latest version!	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa24609	XM_005160329.3	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa24607	XM_005160328.3	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa24608	XM_002665642.5	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH73-212J7.1 ( XM_005160328.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160385.1 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.

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CloneID	ODa24607
Clone ID Related Accession (Same CDS sequence)	XM_005160328.4 , XM_005160328.3
Accession Version	XM_005160328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160328.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGAGA CCATATGCAT CATTACAGAG TTTCCTGTTA 
CCTCAGCTGG TACAGCATAT ACCCGAGGCA AGTTTGAACT ATCAAAAGGT TCCTCCAGGC 
TCAACTAGTC CAATAGACCA GGACTCAAAT ATGAACGACC ATGACAGTTC CAGACGGCGT 
CCCTGGAACC GTGAAGAGGT AATACTGGAC AATCTCATGT TGAATCCAGT TTCCCAGCTC 
TCCCAGGCTA TCAGGGAGAA CACGGAGCAA CTAGCTGAGA AAATGAAAGT TCTGTTTCAC 
AATAAGACCG AGGTCTGGGA GGAAATAGAG GCAAAAATTA ATGCTGAAAA TGAAGTTCCA 
ATCATTAAGA CATCTAATAA GGAAATTTCA TCTATTCTTC AAGAACTGCG AAGGGTCCAA 
AGACAACTGG AGGTGATCAA CACTATTGTT GAGCCTGGTG GGATCCTCAA GATGCCCCCT 
AAGGTAACTT CTGGGGGAAA GATCAAGTCT GCTCCCAAGG AGTTTACCAG CTCCTCTTCT 
ACTAATGCCA ATGCAAGTGC TAAGCAAGGC CCCAGAGGGT CTGGAGGAGG GCGATATGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160385.1
CDS	272..5374
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREERPYASLQSFLLPQLVQHIPEASLNYQKVPPGSTSPIDQDSNMNDHDSSRRRPWNREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_005160328.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160328.4

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGAGA CCATATGCAT CATTACAGAG TTTCCTGTTA 
CCTCAGCTGG TACAGCATAT ACCCGAGGCA AGTTTGAACT ATCAAAAGGT TCCTCCAGGC 
TCAACTAGTC CAATAGACCA GGACTCAAAT ATGAACGACC ATGACAGTTC CAGACGGCGT 
CCCTGGAACC GTGAAGAGGT AATACTGGAC AATCTCATGT TGAATCCAGT TTCCCAGCTC 
TCCCAGGCTA TCAGGGAGAA CACGGAGCAA CTAGCTGAGA AAATGAAAGT TCTGTTTCAC 
AATAAGACCG AGGTCTGGGA GGAAATAGAG GCAAAAATTA ATGCTGAAAA TGAAGTTCCA 
ATCATTAAGA CATCTAATAA GGAAATTTCA TCTATTCTTC AAGAACTGCG AAGGGTCCAA 
AGACAACTGG AGGTGATCAA CACTATTGTT GAGCCTGGTG GGATCCTCAA GATGCCCCCT 
AAGGTAACTT CTGGGGGAAA GATCAAGTCT GCTCCCAAGG AGTTTACCAG CTCCTCTTCT 
ACTAATGCCA ATGCAAGTGC TAAGCAAGGC CCCAGAGGGT CTGGAGGAGG GCGATATGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH73-212J7.1 ( XM_005160328.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160385.1 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.

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CloneID	ODa24607
Clone ID Related Accession (Same CDS sequence)	XM_005160328.4 , XM_005160328.3
Accession Version	XM_005160328.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGAGA CCATATGCAT CATTACAGAG TTTCCTGTTA 
CCTCAGCTGG TACAGCATAT ACCCGAGGCA AGTTTGAACT ATCAAAAGGT TCCTCCAGGC 
TCAACTAGTC CAATAGACCA GGACTCAAAT ATGAACGACC ATGACAGTTC CAGACGGCGT 
CCCTGGAACC GTGAAGAGGT AATACTGGAC AATCTCATGT TGAATCCAGT TTCCCAGCTC 
TCCCAGGCTA TCAGGGAGAA CACGGAGCAA CTAGCTGAGA AAATGAAAGT TCTGTTTCAC 
AATAAGACCG AGGTCTGGGA GGAAATAGAG GCAAAAATTA ATGCTGAAAA TGAAGTTCCA 
ATCATTAAGA CATCTAATAA GGAAATTTCA TCTATTCTTC AAGAACTGCG AAGGGTCCAA 
AGACAACTGG AGGTGATCAA CACTATTGTT GAGCCTGGTG GGATCCTCAA GATGCCCCCT 
AAGGTAACTT CTGGGGGAAA GATCAAGTCT GCTCCCAAGG AGTTTACCAG CTCCTCTTCT 
ACTAATGCCA ATGCAAGTGC TAAGCAAGGC CCCAGAGGGT CTGGAGGAGG GCGATATGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160385.1
CDS	251..5353
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREERPYASLQSFLLPQLVQHIPEASLNYQKVPPGSTSPIDQDSNMNDHDSSRRRPWNREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_005160328.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160328.3

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGAGA CCATATGCAT CATTACAGAG TTTCCTGTTA 
CCTCAGCTGG TACAGCATAT ACCCGAGGCA AGTTTGAACT ATCAAAAGGT TCCTCCAGGC 
TCAACTAGTC CAATAGACCA GGACTCAAAT ATGAACGACC ATGACAGTTC CAGACGGCGT 
CCCTGGAACC GTGAAGAGGT AATACTGGAC AATCTCATGT TGAATCCAGT TTCCCAGCTC 
TCCCAGGCTA TCAGGGAGAA CACGGAGCAA CTAGCTGAGA AAATGAAAGT TCTGTTTCAC 
AATAAGACCG AGGTCTGGGA GGAAATAGAG GCAAAAATTA ATGCTGAAAA TGAAGTTCCA 
ATCATTAAGA CATCTAATAA GGAAATTTCA TCTATTCTTC AAGAACTGCG AAGGGTCCAA 
AGACAACTGG AGGTGATCAA CACTATTGTT GAGCCTGGTG GGATCCTCAA GATGCCCCCT 
AAGGTAACTT CTGGGGGAAA GATCAAGTCT GCTCCCAAGG AGTTTACCAG CTCCTCTTCT 
ACTAATGCCA ATGCAAGTGC TAAGCAAGGC CCCAGAGGGT CTGGAGGAGG GCGATATGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH73-212J7.1 ( XM_002665642.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665688.3 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24608
Clone ID Related Accession (Same CDS sequence)	XM_002665642.6 , XM_002665642.5
Accession Version	XM_002665642.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665642.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGCTG GTACAGCATA TACCCGAGGC AAGTTTGAAC 
TATCAAAAGG TTCCTCCAGG CTCAACTAGT CCAATAGACC AGGACTCAAA TATGAACGAC 
CATGACAGTT CCAGACGGCG TCCCTGGAAC CGTGAAGAGG TAATACTGGA CAATCTCATG 
TTGAATCCAG TTTCCCAGCT CTCCCAGGCT ATCAGGGAGA ACACGGAGCA ACTAGCTGAG 
AAAATGAAAG TTCTGTTTCA CAATAAGACC GAGGTCTGGG AGGAAATAGA GGCAAAAATT 
AATGCTGAAA ATGAAGTTCC AATCATTAAG ACATCTAATA AGGAAATTTC ATCTATTCTT 
CAAGAACTGC GAAGGGTCCA AAGACAACTG GAGGTGATCA ACACTATTGT TGAGCCTGGT 
GGGATCCTCA AGATGCCCCC TAAGGTAACT TCTGGGGGAA AGATCAAGTC TGCTCCCAAG 
GAGTTTACCA GCTCCTCTTC TACTAATGCC AATGCAAGTG CTAAGCAAGG CCCCAGAGGG 
TCTGGAGGAG GGCGATATGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665688.3
CDS	271..5334
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREELVQHIPEASLNYQKVPPGSTSPIDQDSNMNDHDSSRRRPWNREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_002665642.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665642.6

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGCTG GTACAGCATA TACCCGAGGC AAGTTTGAAC 
TATCAAAAGG TTCCTCCAGG CTCAACTAGT CCAATAGACC AGGACTCAAA TATGAACGAC 
CATGACAGTT CCAGACGGCG TCCCTGGAAC CGTGAAGAGG TAATACTGGA CAATCTCATG 
TTGAATCCAG TTTCCCAGCT CTCCCAGGCT ATCAGGGAGA ACACGGAGCA ACTAGCTGAG 
AAAATGAAAG TTCTGTTTCA CAATAAGACC GAGGTCTGGG AGGAAATAGA GGCAAAAATT 
AATGCTGAAA ATGAAGTTCC AATCATTAAG ACATCTAATA AGGAAATTTC ATCTATTCTT 
CAAGAACTGC GAAGGGTCCA AAGACAACTG GAGGTGATCA ACACTATTGT TGAGCCTGGT 
GGGATCCTCA AGATGCCCCC TAAGGTAACT TCTGGGGGAA AGATCAAGTC TGCTCCCAAG 
GAGTTTACCA GCTCCTCTTC TACTAATGCC AATGCAAGTG CTAAGCAAGG CCCCAGAGGG 
TCTGGAGGAG GGCGATATGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH73-212J7.1 ( XM_002665642.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665688.3 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.

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CloneID	ODa24608
Clone ID Related Accession (Same CDS sequence)	XM_002665642.6 , XM_002665642.5
Accession Version	XM_002665642.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665642.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGCTG GTACAGCATA TACCCGAGGC AAGTTTGAAC 
TATCAAAAGG TTCCTCCAGG CTCAACTAGT CCAATAGACC AGGACTCAAA TATGAACGAC 
CATGACAGTT CCAGACGGCG TCCCTGGAAC CGTGAAGAGG TAATACTGGA CAATCTCATG 
TTGAATCCAG TTTCCCAGCT CTCCCAGGCT ATCAGGGAGA ACACGGAGCA ACTAGCTGAG 
AAAATGAAAG TTCTGTTTCA CAATAAGACC GAGGTCTGGG AGGAAATAGA GGCAAAAATT 
AATGCTGAAA ATGAAGTTCC AATCATTAAG ACATCTAATA AGGAAATTTC ATCTATTCTT 
CAAGAACTGC GAAGGGTCCA AAGACAACTG GAGGTGATCA ACACTATTGT TGAGCCTGGT 
GGGATCCTCA AGATGCCCCC TAAGGTAACT TCTGGGGGAA AGATCAAGTC TGCTCCCAAG 
GAGTTTACCA GCTCCTCTTC TACTAATGCC AATGCAAGTG CTAAGCAAGG CCCCAGAGGG 
TCTGGAGGAG GGCGATATGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665688.3
CDS	251..5314
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREELVQHIPEASLNYQKVPPGSTSPIDQDSNMNDHDSSRRRPWNREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_002665642.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665642.5

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGCTG GTACAGCATA TACCCGAGGC AAGTTTGAAC 
TATCAAAAGG TTCCTCCAGG CTCAACTAGT CCAATAGACC AGGACTCAAA TATGAACGAC 
CATGACAGTT CCAGACGGCG TCCCTGGAAC CGTGAAGAGG TAATACTGGA CAATCTCATG 
TTGAATCCAG TTTCCCAGCT CTCCCAGGCT ATCAGGGAGA ACACGGAGCA ACTAGCTGAG 
AAAATGAAAG TTCTGTTTCA CAATAAGACC GAGGTCTGGG AGGAAATAGA GGCAAAAATT 
AATGCTGAAA ATGAAGTTCC AATCATTAAG ACATCTAATA AGGAAATTTC ATCTATTCTT 
CAAGAACTGC GAAGGGTCCA AAGACAACTG GAGGTGATCA ACACTATTGT TGAGCCTGGT 
GGGATCCTCA AGATGCCCCC TAAGGTAACT TCTGGGGGAA AGATCAAGTC TGCTCCCAAG 
GAGTTTACCA GCTCCTCTTC TACTAATGCC AATGCAAGTG CTAAGCAAGG CCCCAGAGGG 
TCTGGAGGAG GGCGATATGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH73-212J7.1 ( XM_005160329.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160386.1 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.

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CloneID	ODa24609
Clone ID Related Accession (Same CDS sequence)	XM_005160329.4 , XM_005160329.3
Accession Version	XM_005160329.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160329.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGGTA ATACTGGACA ATCTCATGTT GAATCCAGTT 
TCCCAGCTCT CCCAGGCTAT CAGGGAGAAC ACGGAGCAAC TAGCTGAGAA AATGAAAGTT 
CTGTTTCACA ATAAGACCGA GGTCTGGGAG GAAATAGAGG CAAAAATTAA TGCTGAAAAT 
GAAGTTCCAA TCATTAAGAC ATCTAATAAG GAAATTTCAT CTATTCTTCA AGAACTGCGA 
AGGGTCCAAA GACAACTGGA GGTGATCAAC ACTATTGTTG AGCCTGGTGG GATCCTCAAG 
ATGCCCCCTA AGGTAACTTC TGGGGGAAAG ATCAAGTCTG CTCCCAAGGA GTTTACCAGC 
TCCTCTTCTA CTAATGCCAA TGCAAGTGCT AAGCAAGGCC CCAGAGGGTC TGGAGGAGGG 
CGATATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160386.1
CDS	272..5203
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_005160329.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160329.4

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGGTA ATACTGGACA ATCTCATGTT GAATCCAGTT 
TCCCAGCTCT CCCAGGCTAT CAGGGAGAAC ACGGAGCAAC TAGCTGAGAA AATGAAAGTT 
CTGTTTCACA ATAAGACCGA GGTCTGGGAG GAAATAGAGG CAAAAATTAA TGCTGAAAAT 
GAAGTTCCAA TCATTAAGAC ATCTAATAAG GAAATTTCAT CTATTCTTCA AGAACTGCGA 
AGGGTCCAAA GACAACTGGA GGTGATCAAC ACTATTGTTG AGCCTGGTGG GATCCTCAAG 
ATGCCCCCTA AGGTAACTTC TGGGGGAAAG ATCAAGTCTG CTCCCAAGGA GTTTACCAGC 
TCCTCTTCTA CTAATGCCAA TGCAAGTGCT AAGCAAGGCC CCAGAGGGTC TGGAGGAGGG 
CGATATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH73-212J7.1 ( XM_005160329.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160386.1 Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.

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CloneID	ODa24609
Clone ID Related Accession (Same CDS sequence)	XM_005160329.4 , XM_005160329.3
Accession Version	XM_005160329.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160329.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGGTA ATACTGGACA ATCTCATGTT GAATCCAGTT 
TCCCAGCTCT CCCAGGCTAT CAGGGAGAAC ACGGAGCAAC TAGCTGAGAA AATGAAAGTT 
CTGTTTCACA ATAAGACCGA GGTCTGGGAG GAAATAGAGG CAAAAATTAA TGCTGAAAAT 
GAAGTTCCAA TCATTAAGAC ATCTAATAAG GAAATTTCAT CTATTCTTCA AGAACTGCGA 
AGGGTCCAAA GACAACTGGA GGTGATCAAC ACTATTGTTG AGCCTGGTGG GATCCTCAAG 
ATGCCCCCTA AGGTAACTTC TGGGGGAAAG ATCAAGTCTG CTCCCAAGGA GTTTACCAGC 
TCCTCTTCTA CTAATGCCAA TGCAAGTGCT AAGCAAGGCC CCAGAGGGTC TGGAGGAGGG 
CGATATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160386.1
CDS	251..5182
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYNPATDEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDIVRFGYDSHVYILERSQHKVPEEALKHEKYTSQLQMGIKCEEGKQQNVQEEKSKLERSDRKSLTESPVPRPTPLYGQPSWWGEEDSGNMENSEVRRSSDNHSDITKEIHTAEDDFNLQEIHDGQAKSTYLYNREPSYFEIPTKEFHVQHKSPTTDLIDIPTKDTNVSLISTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSSRQRSKQQQPGNTLAATPTEVLSAESKVADWLVQSDVSMMCRRTPCEDVYSTKSDLAMHIRTLKGHHHDDGTQSDSEDPVMKGKCSKSHHSMQSVQSYHSTQSKTSEPSESLLSQQSVIPQKSHNPQTLPQHKHHQVKLDESEDRPIQEGSSPPRTEVEPSHTEPHTQQAFIIEFFDDNPRKKRSQSFTHNSVHADSYSALKAKLERRKGLAHGERPASVHGLVPPTQQITVPLKGASHGGSQRSSSLKREKTEDTGSSSSLLGSSSRSSPTITIKPFGSMGKNSKLAQEFTEEFSKDTGKIFLPPKSAPPVMMSPSHTPSSSLAETSGHVSVSYPSSPCQHSALPQSLLSASSPVSAPSHIPGRSPVFSASPVCSSVPQVSPQVVSCVDTKASRVTRTEEEDSLSDAGTYTIETESQDKEVEEARSMIDQVFGVLDAPEYSGQTLGVYRPVIDDGDNEQLFFSQSHSNAADVMSTSSLGHGRDQVISLQAHPGVVGPKWVSRWASLADPYSDPGATQGSVPVPPQSAERDDRVTLIIGQCLDISESEGSQSSRTRRLLPQVPPGEKLENTTPSILIRHETNLDHEPPEKGSRMPQQQDNTQRLRVQDDVDPDSLSDTSRSDDSSILDRSGRSQGKRGTTRHLPSENVNHSRELREMPQPTPKPTSFYIGSEDGLSKSDVSKSPVLSQPEKDSAFKIPPTTVLIRHLSGHESRRPVKPNSSAPDLQTHNKDVVPTKDTSTSFVRQESFTKDRPSDNIQVKKLPHISSHPALRSLDTEETLQDTFTFLKEVEKSPEEKLSGCSQRSKKGSSPAQEDSFSGDSDVDTASTVSMVSSKNAPVSSLKKHTSANDTGKEKSSSGLPAKHKARQPTARERLSEKRKSHASADNTSKAELSRRLQLRRSAGNRGSLDLSDNQHGQQLWPEAGASDHESSSRPNNRKKLTAPLQKEDPNKVTKGSHQVLTRSNSLSAPRPTRASMLRRARLGEASDNESAETDRASPSSEHSKTESKKLSRLDILAMPRKRTRSFTVPSDNESTSSKHGISNRTTEASSSARKGASSEVRHANSKGTAAAGKHSSRTHSTQVKHSSTAGSHRRQRDSDYSSTSEEEFETKTNCKQKRSHASASTQTQTPQKTAQMRLKCGSLETEEDETQNEHFQNWSTHSAEIARLSQDLAKDLAILAREIHDVAGDGDSQASSGIGATTSPVSMPNTPASTISTREEVILDNLMLNPVSQLSQAIRENTEQLAEKMKVLFHNKTEVWEEIEAKINAENEVPIIKTSNKEISSILQELRRVQRQLEVINTIVEPGGILKMPPKVTSGGKIKSAPKEFTSSSSTNANASAKQGPRGSGGGRYV

Target ORF information:

RefSeq Version	XM_005160329.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-212j7.1 (si:ch73-212j7.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160329.3

ORF Insert Sequence:

ATGAGTGTGA CATCATGGTT TTTGGTCAGC AGCTCTGGCA CTCGTCACCG ACTGCCTCGG 
GAAATGATCT TTGTTGGCCG GGAGGACTGT GAGCTCATGC TGCAGTCTCG GAGTGTAGAT 
AAACAGCATG CTGTTATCAA CTATAACCCT GCCACAGATG AGCACCTCGT CAAGGACCTT 
GGAAGTTTAA ATGGGACTTT TGTCAATGAC TTGAGAATTC CCGACCAAAC ATATATAACC 
CTCAAGCTGT CAGACATTGT TCGTTTTGGA TATGATTCAC ATGTGTATAT TCTTGAGAGG 
AGTCAACATA AAGTCCCTGA GGAGGCACTC AAGCACGAGA AGTACACCAG TCAGCTACAG 
ATGGGCATCA AATGTGAAGA GGGAAAACAG CAGAATGTGC AAGAGGAAAA GTCTAAATTA 
GAGCGCTCTG ATCGTAAGAG TCTCACAGAA TCCCCTGTAC CCCGACCAAC ACCACTGTAT 
GGCCAGCCAT CATGGTGGGG TGAGGAGGAC TCTGGGAACA TGGAAAACAG TGAAGTACGA 
AGGTCAAGTG ACAATCATTC AGACATCACA AAGGAGATCC ACACAGCAGA GGATGATTTT 
AACCTTCAGG AGATCCATGA TGGCCAGGCA AAGTCAACGT ACCTGTATAA CAGAGAGCCC 
AGCTACTTTG AAATTCCAAC TAAGGAGTTT CACGTGCAAC ACAAGTCGCC AACGACAGAC 
TTGATTGACA TCCCAACCAA AGATACAAAT GTTTCTCTGA TCTCCACACC ACCAGTTGTC 
CAAAGTCATG CTTCCTTTAC TATCGAGTTT GACGATTGCA TGCCAGGAAA GATTAAAATC 
AAGGATCATG TGACCAAGTT CTCGTCCCGT CAACGTAGCA AACAGCAGCA GCCCGGTAAT 
ACTCTGGCAG CCACTCCCAC GGAGGTTTTG TCAGCTGAGA GCAAGGTGGC GGATTGGCTC 
GTGCAGAGCG ATGTCAGCAT GATGTGCAGG AGGACACCCT GTGAGGACGT ATACAGCACT 
AAGAGTGACT TGGCGATGCA TATTCGGACA CTCAAAGGAC ACCATCATGA CGATGGAACC 
CAGAGTGATT CTGAAGACCC TGTGATGAAA GGAAAATGCA GCAAGTCACA TCATTCCATG 
CAATCAGTAC AATCATACCA CTCAACTCAA TCGAAGACAT CCGAACCATC AGAATCACTT 
TTGTCCCAGC AGTCTGTCAT ACCACAAAAA TCCCATAATC CACAAACACT CCCACAACAC 
AAACACCACC AAGTAAAGCT TGATGAAAGC GAAGATCGTC CCATCCAAGA AGGCTCCTCT 
CCTCCTAGAA CAGAGGTCGA GCCCAGTCAC ACTGAGCCTC ACACTCAACA GGCCTTCATT 
ATCGAGTTTT TTGATGATAA TCCCAGAAAG AAGCGATCGC AGTCCTTTAC CCACAATTCT 
GTGCATGCTG ACTCCTACTC TGCACTGAAG GCCAAACTAG AGAGGCGCAA AGGTCTGGCT 
CATGGAGAAA GGCCCGCCTC GGTGCATGGA CTTGTTCCTC CAACACAGCA AATCACTGTT 
CCCCTGAAAG GAGCAAGCCA TGGAGGTTCA CAGCGATCAA GCTCCTTGAA AAGGGAGAAG 
ACAGAAGACA CTGGGAGCAG CTCATCCCTA TTAGGGAGCT CCTCTCGCTC TTCACCAACT 
ATCACCATTA AGCCTTTTGG AAGCATGGGT AAAAATTCAA AACTAGCTCA GGAATTTACA 
GAAGAATTCT CCAAGGATAC TGGAAAGATT TTCTTACCAC CTAAGTCTGC TCCACCAGTG 
ATGATGTCCC CATCACACAC TCCTTCATCC TCCCTGGCAG AGACCTCTGG ACATGTATCT 
GTATCATATC CATCTTCTCC GTGCCAGCAC TCTGCATTGC CCCAGTCTCT TCTCTCAGCA 
TCATCGCCTG TCTCTGCTCC TTCCCACATC CCTGGAAGGA GTCCGGTTTT TAGTGCCTCA 
CCAGTCTGCT CATCGGTGCC CCAAGTTTCA CCACAGGTAG TTAGCTGTGT TGACACGAAG 
GCATCAAGGG TAACGCGAAC CGAAGAGGAG GACAGTCTGA GTGATGCTGG TACCTACACA 
ATTGAGACTG AGTCACAAGA TAAGGAAGTG GAAGAAGCTA GAAGTATGAT TGACCAGGTG 
TTTGGTGTTT TAGATGCGCC AGAATACAGT GGTCAGACTC TTGGAGTTTA TAGGCCTGTG 
ATTGATGATG GGGACAATGA GCAGCTATTC TTTAGTCAAA GCCACAGTAA TGCAGCTGAT 
GTAATGTCAA CATCATCATT GGGCCATGGC AGGGACCAAG TCATCTCATT GCAGGCACAT 
CCTGGTGTAG TAGGGCCTAA ATGGGTCTCG CGTTGGGCCA GCCTTGCAGA CCCTTATAGT 
GATCCCGGGG CTACACAAGG ATCTGTACCA GTTCCACCCC AAAGTGCAGA AAGAGATGAC 
AGAGTGACAT TAATTATTGG TCAATGCTTG GATATCTCAG AATCAGAGGG AAGTCAAAGT 
TCCAGGACCA GACGGCTACT TCCTCAGGTT CCTCCTGGAG AAAAACTAGA AAACACAACC 
CCTAGCATCC TAATCCGACA TGAAACAAAC TTGGACCATG AGCCACCAGA GAAAGGTTCC 
AGAATGCCAC AGCAGCAAGA CAACACTCAG AGGCTACGTG TCCAGGATGA TGTGGACCCA 
GACAGTCTGA GTGACACCAG CCGTTCAGAT GACAGCTCTA TCCTTGATAG AAGCGGTAGG 
AGCCAAGGAA AACGAGGAAC TACAAGACAC CTACCTTCAG AAAATGTAAA CCATTCAAGA 
GAGCTGAGGG AGATGCCACA ACCTACACCC AAACCCACTT CATTTTATAT CGGTTCTGAG 
GATGGTTTAT CCAAGTCCGA TGTGTCCAAA AGCCCAGTTC TTTCACAGCC AGAGAAAGAC 
TCTGCTTTCA AGATACCTCC AACTACTGTC TTGATACGAC ATTTGAGTGG CCATGAGTCT 
AGACGTCCAG TGAAACCCAA CTCATCAGCT CCAGATCTTC AGACACATAA CAAGGATGTG 
GTGCCCACCA AAGATACTTC AACCTCATTT GTTCGACAGG AGAGTTTTAC CAAAGACAGG 
CCAAGTGACA ACATTCAAGT CAAGAAACTG CCACATATCT CTAGTCATCC TGCTTTGCGA 
AGCTTGGATA CAGAGGAGAC ACTTCAAGAT ACATTTACCT TTCTTAAGGA AGTAGAGAAA 
TCTCCAGAGG AGAAGTTGTC TGGATGTAGT CAACGATCAA AAAAAGGAAG CTCTCCTGCA 
CAAGAAGACT CCTTTTCTGG TGACTCAGAT GTGGATACTG CCAGTACTGT AAGCATGGTC 
AGCAGTAAAA ATGCCCCTGT CAGTTCACTT AAGAAGCACA CGTCTGCAAA TGACACGGGT 
AAGGAGAAGT CATCATCTGG TTTACCTGCA AAACACAAAG CACGACAACC CACAGCTCGC 
GAACGACTGT CAGAGAAACG CAAAAGTCAT GCCTCTGCAG ATAACACCAG TAAAGCTGAA 
CTAAGTCGTC GGCTCCAGCT TCGCCGCAGT GCTGGCAATC GTGGTTCGTT AGACCTTTCA 
GATAATCAGC ATGGTCAGCA GCTATGGCCT GAAGCGGGTG CTTCCGACCA TGAGTCAAGT 
TCACGGCCAA ACAACCGTAA AAAACTCACT GCTCCCCTGC AGAAGGAGGA CCCCAATAAG 
GTGACCAAAG GAAGCCATCA GGTGCTGACC AGATCCAACA GTCTTTCAGC CCCAAGACCA 
ACACGTGCCT CCATGCTCCG CCGAGCTCGA CTAGGTGAGG CTTCAGATAA TGAGAGCGCT 
GAGACAGACC GGGCATCTCC AAGCTCTGAG CACAGCAAGA CAGAATCGAA AAAACTCTCA 
AGGCTAGACA TTCTTGCCAT GCCTCGGAAA CGCACAAGGT CCTTCACTGT TCCAAGTGAC 
AATGAGTCAA CTTCAAGTAA GCATGGCATT TCTAACCGTA CTACAGAGGC AAGTAGCAGT 
GCAAGGAAAG GTGCTTCAAG TGAGGTGAGG CATGCTAACA GCAAAGGGAC TGCAGCAGCT 
GGGAAACACT CCTCCCGTAC CCATTCTACC CAAGTTAAAC ACAGTAGCAC AGCTGGCTCC 
CACCGTAGAC AAAGGGATTC TGACTATTCC TCAACCTCAG AGGAAGAGTT TGAGACTAAG 
ACCAACTGTA AACAAAAACG GTCCCATGCA TCAGCTTCAA CTCAGACACA GACTCCGCAG 
AAGACCGCGC AAATGAGGCT AAAATGTGGC TCTTTGGAAA CAGAAGAGGA TGAAACTCAG 
AACGAACACT TCCAGAACTG GTCAACTCAT AGCGCAGAGA TTGCCAGGCT AAGTCAAGAT 
CTTGCTAAAG ATCTTGCCAT TCTTGCTCGT GAAATCCATG ATGTAGCAGG CGATGGTGAT 
TCCCAGGCAT CCTCAGGCAT AGGAGCTACC ACTTCCCCAG TCTCCATGCC CAATACCCCT 
GCATCCACTA TCTCAACCAG AGAGGAGGTA ATACTGGACA ATCTCATGTT GAATCCAGTT 
TCCCAGCTCT CCCAGGCTAT CAGGGAGAAC ACGGAGCAAC TAGCTGAGAA AATGAAAGTT 
CTGTTTCACA ATAAGACCGA GGTCTGGGAG GAAATAGAGG CAAAAATTAA TGCTGAAAAT 
GAAGTTCCAA TCATTAAGAC ATCTAATAAG GAAATTTCAT CTATTCTTCA AGAACTGCGA 
AGGGTCCAAA GACAACTGGA GGTGATCAAC ACTATTGTTG AGCCTGGTGG GATCCTCAAG 
ATGCCCCCTA AGGTAACTTC TGGGGGAAAG ATCAAGTCTG CTCCCAAGGA GTTTACCAGC 
TCCTCTTCTA CTAATGCCAA TGCAAGTGCT AAGCAAGGCC CCAGAGGGTC TGGAGGAGGG 
CGATATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI:CH73-212J7.1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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