ulk1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ulk1a
Entrez Gene ID	100170793
Full Name	unc-51 like autophagy activating kinase 1a
Synonyms	zgc:195008
General protein information	Preferred Names unc-51 like autophagy activating kinase 1a Names unc-51 like autophagy activating kinase 1a unc-51-like kinase 1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130631.1	NP_001124103.1	unc-51 like autophagy activating kinase 1a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ulk1a	NP_001124103.1
Caenorhabditis elegans (roundworm)	unc-51	NP_507869.1

Gene Ontology
Bibliography

Related articles in PubMed

Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes.
Biga PR, Latimer MN, Froehlich JM, Gabillard JC, Seiliez I
Biology open6(11)1720-1725(2017 Nov)

The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erb? and indirectly via Cebpb/(C/ebp?) in zebrafish.
Huang G, Zhang F, Ye Q, Wang H
Autophagy12(8)1292-309(2016 Aug)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos.
He C, Bartholomew CR, Zhou W, Klionsky DJ
Autophagy5(4)520-6(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ulk1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ulk1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03436	NM_001130631.1 Latest version!	Danio rerio unc-51 like autophagy activating kinase 1a (ulk1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ulk1a ( NM_001130631.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124103.1 Danio rerio unc-51 like autophagy activating kinase 1a (ulk1a), mRNA.

Price & Availability↑

CloneID	ODa03436
Clone ID Related Accession (Same CDS sequence)	NM_001130631.1
Accession Version	NM_001130631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	unc-51 like autophagy activating kinase 1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163488.1. ##Evidence-Data-START## Transcript exon combination :: BC163488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAGCA TCGGAAAGTA CGAGTTCAAC AGGAAAGACT TGATCGGACA TGGCGCATTT 
GCTGTGGTGT TCAAAGGAAG ACACAAACAG AAACATAATT TTGAAGTCGC GGTGAAATGC 
ATTAACAGGA AAAATCTCGC CAAATCACAG TCACTCCTAG GAAAGGAAAT CAAAATATTA 
AAGGAGCTGA AACATGAGAA TATTGTCAGT TTGCTGGACT TTCAGGAGAT TTCAGGATGC 
GTCTATCTTG TCATGGAGTA TTGCAATGGA GGTGACTTAG CAGAGTATTT ACACTCAAAA 
GGCTGTCTGA GTGAGGACAC CATCCGTGTG TTACTGCAGC AGCTGGCAGG AGCTATGAGT 
GTTTTAAGGA GTAAAGGCAT CATTCATCGA GACCTGAAGC CCCAAAACAT CCTTCTCTCA 
TACAGCACAG GTCGCAAATC CAATCCCAAC AACATCTGCA TTAAACTGGC TGATTTTGGC 
TTTGCACGGT ATCTGCAGGG AAACACAATG GCTGCCACAC TATGTGGATC TCCAATGTAC 
ATGGCTCCAG AAGTCATCAT GTCTCATAAT TATGATGCCA AAGCTGATCT GTGGAGTGTG 
GGGACCATCA TCTACCAGTG CTTGACTGGC AAAGCCCCAT TTCAGGCAAG TACTCCTCAG 
GAACTGCGGC AGTTCTATGA AAGAAACCGG AGCCTCAGTC CCAGTATTCC ACGTGAAACT 
TCCAGTCACT TGAGGCACCT TCTGCTTGGG TTGCTGCAGA GAAACCAAAG GGAACGCATA 
GACTTTGATG ATTTTTTTCA CCACCCTTTC CTGGAGGCCA GTACATCTAT GAAGAAATCA 
TCTCCTGTGC CGGTGCCTGT GCCCGTGCCC ACATATCCCA GCTCAGGCTC TGGAAGCTTC 
AGCAGTGGCT CCTCGACCTC TCGCCTCACT CCTACACAGT CTGATACTGA AGCTCCAAAG 
TCCCTCCCTC AAATGAAGCC CACAGAGTCT CCTGGATCCC TGCTGAAAGA CTCCTCTTCC 
TCCGACTCGG ATGACTTTGT TATGGTTCCG GCTCAGTTCA CCAGTGAGCC AGTCGGTGCA 
GAAGGGCAGG ACAGCTTGAT GCGCAGCGGA AGCTCACTGC TGGGTGCCAC TGCTTGTTTG 
TGTCAAACAC CATCGTCCTC TCCATCTGGC AGAACATCCC CGAGACCCTC CAGGCATCTA 
GAGTCGTGTG GCAGTAGCTA CGCTCAGTCT ATTCCTGTTC CAGTGCCAAC ACAAAAGCAC 
AACTACCAGA GGATGGAGCA TAATCTACAA GAGTCTGAAA GGACCTCAGC GTCTGCACAT 
CACTGTTGCT CTGATGGATC TCCAGCATGT GGGAGAACCG GTGTTTCTCC AGATTCAGGA 
GGATCCAGAT CGTATTTACC TTCCCCACAG GTCGGAAGCA TTCCTGAAAC ACCCAGTCAG 
CCTGAACCTG TTGGCAGCAG GACAGGGTTT CATGCCACAG ATCAACAGCC TGGTCAAGTG 
TCTCCTCTCT TCACTGACCT CACAGACTTG GACCCAACAC TGGCCCAAAG ACTCAGAGCT 
CATCGCCACA GTCTGGATAC CATAGTGCAC AGGTGGGATT TGTGTCGGCG TCCCAGCTTT 
GGTCAAACTC CAAATGCAGC ACTGCTTCAG CGAGGCCTCA CCACCCGAAC CAAGACCATG 
TGTGACCTGA AAGATCTGGC ACAGTATCCT GCTGCGGGGC CTCGGGTGCC GCTTGTCAGG 
CCAGCAGAAA GAAGAGACAC CGTCAAAAGG TCAGTGAGTA CCGGCCGTCT CTCGGATAAA 
CTGCTGAAGG CTGTGTTTGG ACATCAGCTT GACAGAGGCA GCTGCGAGAG CTTAAATTCA 
GAGAAACCCA TGGATACAAC TGCGCCTCCT TGTGGTGTTG ATCGCTCAGA CAGTCCTGCT 
GGAGTCATGT TCATGCTGGG CACTCCTGAA TATGGACACA CTCCGCCTCA TGCTAGACAC 
AGACGCTTCT CAGTGGGGGC TCCTTGCTCT ACCAGTCCTG GCTGGACCTA CGGCTCTCCT 
GAAAGCCTGC TCTATGGTTA CACAGACGCC ATGGCAACAA ACCTGCACGA GAATGTGACA 
TTTGTGCCAC CTGAACTGCC AGAGGAGACG TTAATGGAGG AGGGACACAC GGACTTGCTG 
AGCCAGTTGC GCTTCGTTCT AGCCTTTTCC CACTGCATAA CAGAACTGGC TGAAACAAAG 
GACATCGGGA CTGGAAGAAA CGTGCCACCT AATGCTTCAC TCTTAGAGCA GAGTGTAGTG 
GCTGACCAGA TCAGCCTGCT GAGCCGAGAG TGGAGCTCTG CAGAACAATT GGTTCTCTAT 
ATGAAATGTG CTGAGCTGCT GTCTTCTGCA CTACACACAG CGATGGCTGG CATCAAAGAA 
GGAAAACTCT ACCCATCAGG ATCTGTGAAA CAAGTGGTGC GTGAGCTGAA TGAGATGTAT 
AAGGAATGTG TGAACTCCTG TCGTTCTCTG ACTGAGAAAC TCCAGCACTT CTTCAGCAGC 
AAACAGAGAC TGATGGACCG GATGAACAGC ATCACCGCTG AGAGGCTCAT CTACGCACAC 
ACCGTTCAGA TGGTACAGAC GGCAGCACTA GACGAGATGT TTCATCACGG AGAAAGCTCT 
CTAGAGAGAT ACCACAAGGC CTTACTGCTG ATGGAGGGCC TGTCCTTGAT TATTACAGAG 
CCTTCAGACC TAAACAATGT CAACAAATGT AAACGGTGTA TCGAGCGACG GCTCTGTTCC 
CTCCAAGCCC AAGGATGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124103.1
CDS	288..3071
Translation	MESIGKYEFNRKDLIGHGAFAVVFKGRHKQKHNFEVAVKCINRKNLAKSQSLLGKEIKILKELKHENIVSLLDFQEISGCVYLVMEYCNGGDLAEYLHSKGCLSEDTIRVLLQQLAGAMSVLRSKGIIHRDLKPQNILLSYSTGRKSNPNNICIKLADFGFARYLQGNTMAATLCGSPMYMAPEVIMSHNYDAKADLWSVGTIIYQCLTGKAPFQASTPQELRQFYERNRSLSPSIPRETSSHLRHLLLGLLQRNQRERIDFDDFFHHPFLEASTSMKKSSPVPVPVPVPTYPSSGSGSFSSGSSTSRLTPTQSDTEAPKSLPQMKPTESPGSLLKDSSSSDSDDFVMVPAQFTSEPVGAEGQDSLMRSGSSLLGATACLCQTPSSSPSGRTSPRPSRHLESCGSSYAQSIPVPVPTQKHNYQRMEHNLQESERTSASAHHCCSDGSPACGRTGVSPDSGGSRSYLPSPQVGSIPETPSQPEPVGSRTGFHATDQQPGQVSPLFTDLTDLDPTLAQRLRAHRHSLDTIVHRWDLCRRPSFGQTPNAALLQRGLTTRTKTMCDLKDLAQYPAAGPRVPLVRPAERRDTVKRSVSTGRLSDKLLKAVFGHQLDRGSCESLNSEKPMDTTAPPCGVDRSDSPAGVMFMLGTPEYGHTPPHARHRRFSVGAPCSTSPGWTYGSPESLLYGYTDAMATNLHENVTFVPPELPEETLMEEGHTDLLSQLRFVLAFSHCITELAETKDIGTGRNVPPNASLLEQSVVADQISLLSREWSSAEQLVLYMKCAELLSSALHTAMAGIKEGKLYPSGSVKQVVRELNEMYKECVNSCRSLTEKLQHFFSSKQRLMDRMNSITAERLIYAHTVQMVQTAALDEMFHHGESSLERYHKALLLMEGLSLIITEPSDLNNVNKCKRCIERRLCSLQAQGCV

Target ORF information:

RefSeq Version	NM_001130631.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1a (ulk1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130631.1

ORF Insert Sequence:

ATGGAAAGCA TCGGAAAGTA CGAGTTCAAC AGGAAAGACT TGATCGGACA TGGCGCATTT 
GCTGTGGTGT TCAAAGGAAG ACACAAACAG AAACATAATT TTGAAGTCGC GGTGAAATGC 
ATTAACAGGA AAAATCTCGC CAAATCACAG TCACTCCTAG GAAAGGAAAT CAAAATATTA 
AAGGAGCTGA AACATGAGAA TATTGTCAGT TTGCTGGACT TTCAGGAGAT TTCAGGATGC 
GTCTATCTTG TCATGGAGTA TTGCAATGGA GGTGACTTAG CAGAGTATTT ACACTCAAAA 
GGCTGTCTGA GTGAGGACAC CATCCGTGTG TTACTGCAGC AGCTGGCAGG AGCTATGAGT 
GTTTTAAGGA GTAAAGGCAT CATTCATCGA GACCTGAAGC CCCAAAACAT CCTTCTCTCA 
TACAGCACAG GTCGCAAATC CAATCCCAAC AACATCTGCA TTAAACTGGC TGATTTTGGC 
TTTGCACGGT ATCTGCAGGG AAACACAATG GCTGCCACAC TATGTGGATC TCCAATGTAC 
ATGGCTCCAG AAGTCATCAT GTCTCATAAT TATGATGCCA AAGCTGATCT GTGGAGTGTG 
GGGACCATCA TCTACCAGTG CTTGACTGGC AAAGCCCCAT TTCAGGCAAG TACTCCTCAG 
GAACTGCGGC AGTTCTATGA AAGAAACCGG AGCCTCAGTC CCAGTATTCC ACGTGAAACT 
TCCAGTCACT TGAGGCACCT TCTGCTTGGG TTGCTGCAGA GAAACCAAAG GGAACGCATA 
GACTTTGATG ATTTTTTTCA CCACCCTTTC CTGGAGGCCA GTACATCTAT GAAGAAATCA 
TCTCCTGTGC CGGTGCCTGT GCCCGTGCCC ACATATCCCA GCTCAGGCTC TGGAAGCTTC 
AGCAGTGGCT CCTCGACCTC TCGCCTCACT CCTACACAGT CTGATACTGA AGCTCCAAAG 
TCCCTCCCTC AAATGAAGCC CACAGAGTCT CCTGGATCCC TGCTGAAAGA CTCCTCTTCC 
TCCGACTCGG ATGACTTTGT TATGGTTCCG GCTCAGTTCA CCAGTGAGCC AGTCGGTGCA 
GAAGGGCAGG ACAGCTTGAT GCGCAGCGGA AGCTCACTGC TGGGTGCCAC TGCTTGTTTG 
TGTCAAACAC CATCGTCCTC TCCATCTGGC AGAACATCCC CGAGACCCTC CAGGCATCTA 
GAGTCGTGTG GCAGTAGCTA CGCTCAGTCT ATTCCTGTTC CAGTGCCAAC ACAAAAGCAC 
AACTACCAGA GGATGGAGCA TAATCTACAA GAGTCTGAAA GGACCTCAGC GTCTGCACAT 
CACTGTTGCT CTGATGGATC TCCAGCATGT GGGAGAACCG GTGTTTCTCC AGATTCAGGA 
GGATCCAGAT CGTATTTACC TTCCCCACAG GTCGGAAGCA TTCCTGAAAC ACCCAGTCAG 
CCTGAACCTG TTGGCAGCAG GACAGGGTTT CATGCCACAG ATCAACAGCC TGGTCAAGTG 
TCTCCTCTCT TCACTGACCT CACAGACTTG GACCCAACAC TGGCCCAAAG ACTCAGAGCT 
CATCGCCACA GTCTGGATAC CATAGTGCAC AGGTGGGATT TGTGTCGGCG TCCCAGCTTT 
GGTCAAACTC CAAATGCAGC ACTGCTTCAG CGAGGCCTCA CCACCCGAAC CAAGACCATG 
TGTGACCTGA AAGATCTGGC ACAGTATCCT GCTGCGGGGC CTCGGGTGCC GCTTGTCAGG 
CCAGCAGAAA GAAGAGACAC CGTCAAAAGG TCAGTGAGTA CCGGCCGTCT CTCGGATAAA 
CTGCTGAAGG CTGTGTTTGG ACATCAGCTT GACAGAGGCA GCTGCGAGAG CTTAAATTCA 
GAGAAACCCA TGGATACAAC TGCGCCTCCT TGTGGTGTTG ATCGCTCAGA CAGTCCTGCT 
GGAGTCATGT TCATGCTGGG CACTCCTGAA TATGGACACA CTCCGCCTCA TGCTAGACAC 
AGACGCTTCT CAGTGGGGGC TCCTTGCTCT ACCAGTCCTG GCTGGACCTA CGGCTCTCCT 
GAAAGCCTGC TCTATGGTTA CACAGACGCC ATGGCAACAA ACCTGCACGA GAATGTGACA 
TTTGTGCCAC CTGAACTGCC AGAGGAGACG TTAATGGAGG AGGGACACAC GGACTTGCTG 
AGCCAGTTGC GCTTCGTTCT AGCCTTTTCC CACTGCATAA CAGAACTGGC TGAAACAAAG 
GACATCGGGA CTGGAAGAAA CGTGCCACCT AATGCTTCAC TCTTAGAGCA GAGTGTAGTG 
GCTGACCAGA TCAGCCTGCT GAGCCGAGAG TGGAGCTCTG CAGAACAATT GGTTCTCTAT 
ATGAAATGTG CTGAGCTGCT GTCTTCTGCA CTACACACAG CGATGGCTGG CATCAAAGAA 
GGAAAACTCT ACCCATCAGG ATCTGTGAAA CAAGTGGTGC GTGAGCTGAA TGAGATGTAT 
AAGGAATGTG TGAACTCCTG TCGTTCTCTG ACTGAGAAAC TCCAGCACTT CTTCAGCAGC 
AAACAGAGAC TGATGGACCG GATGAACAGC ATCACCGCTG AGAGGCTCAT CTACGCACAC 
ACCGTTCAGA TGGTACAGAC GGCAGCACTA GACGAGATGT TTCATCACGG AGAAAGCTCT 
CTAGAGAGAT ACCACAAGGC CTTACTGCTG ATGGAGGGCC TGTCCTTGAT TATTACAGAG 
CCTTCAGACC TAAACAATGT CAACAAATGT AAACGGTGTA TCGAGCGACG GCTCTGTTCC 
CTCCAAGCCC AAGGATGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes.
Biology open6(11)1720-1725(2017 Nov)
Biga PR,Latimer MN,Froehlich JM,Gabillard JC,Seiliez I

The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erb? and indirectly via Cebpb/(C/ebp?) in zebrafish.
Autophagy12(8)1292-309(2016 Aug)
Huang G,Zhang F,Ye Q,Wang H

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos.
Autophagy5(4)520-6(2009 May)
He C,Bartholomew CR,Zhou W,Klionsky DJ

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ulk1a cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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