rttn cDNA ORF clone, Danio rerio(zebrafish)

The following rttn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rttn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa57718 XM_017353967.1
Latest version!
Danio rerio rotatin (rttn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa28452 XM_021470297.1
Latest version!
Danio rerio rotatin (rttn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa49009 XM_017353967.2
Latest version!
Danio rerio rotatin (rttn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa28451 XM_005162659.4
Latest version!
Danio rerio rotatin (rttn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa28451 XM_005162659.3 Danio rerio rotatin (rttn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa57718
    Clone ID Related Accession (Same CDS sequence) XM_017353967.1
    Accession Version XM_017353967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rotatin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTTCCT CAGAACATCC AGAATCGTCT CTGATCCACC ACAGAATGGT CTTCACAGGC 
    ACTGCCGTTT TCACAATACG TCTCCTGCAA ACCTTGTTAC CTGTGGAGAA GGCTGCGGAT
    AATCTTCCGG AAAGTACCGT GGCTGCCATT TTCCTGTTGT GTCTTGATAT ATCCTTCAGC
    GTGGCGTTCC CCGTCATCCA TGAGTCAGCC GTGGCGTATC TAGAGCAGGC TTGCTTTGAA
    AGATACAGCC TGTATCGTAG AGTGTCCCGT ATTTCTCATT GCATGGAGGC GACATGCACC
    TTTTTAAAGG ATGCACAGGC TGAGGGCGAT AAGAACTGGC ATGAGCTTTT GGAGCTTGCT
    GACCAAGGAG TAGATGCTTT TCCATATCAT CAGCACCTAC CGGTGGTGAA GGAATGTGTC
    CAGATTTGCT CCTACCTGTG GAAATTTGCA CAAGCAAGCC CGCTTCTGCA GTCGGAGAGT
    CAGAAGATAT TTCTGAAGCT TTTGTCTCAT CCTCTGTCCC CAGTGAAGGC AGAAACCTAC
    TCCTGCACTC TGAGCATTTT GAAGGAGTGT TTGGGAGTTC AGAACGTGGC CAAACCTGCC
    TCATCCATCT GCGCCGGAGT GCATTTCTTG CTTCACTCCC GTGTCCTGTA TGAGATCAGT
    GTGTTTGGCC TTCAAGACCA AGCAACAGAG GTGAGTGCCG CTGCCAAGGA TATTCTGCTT
    TATCTGCTGA AGGGACGATT GATGATGACA GCTGCAAGTT GGAGGAGATT TAATGAGGCA
    CTTTACCCGG TCATTCCTAT ATTACAGAGC TACGCAGGCA CTGAAGACGC CCTGGGAAAC
    TGTATCCTGC TGCTAAGTGA AGCATCTGCT GAGAAAGGAG ATGAGATGCG TTCCCCGACT
    GCTAGGCTAC GGGCAGCCCT CCGGCTTCTG TTCACCAAAC AGCACACCGT GAGGAAAATG
    GCAGTCCAAC ATCTGCTTCC ACATCTGACC AGCGCAGAGG GGGCCCAAAA CTCCAGACCA
    ACCCTGGACA GCTCCACTCT ATCATGTTTA CCAAATCTCT ACAATCTTAA TAAAAGTGTG
    GACATCAAGT TCAGCAGTTC AGACAAGTCA TATGTAAAGG TGGAGTCTGT AAATAAGTTG
    TACAATATTC TCGCATCGGA AACGGTAGAT CTTGTTTTAC GGAAATCTGC AGCTGAACAG
    TTGGCTATTG TTCTGCAAGA TTCATCAATG CACGCGGTGC TGAAGGCCTT GGGTTTATCA
    GACAAGCTCA CGTCTTTCAT CACAGAGTGT GTCAACAGCA ATGTGCCGAG CATGGATTGC
    TTGTTGGAGT CCAGTGCATG CATGCTGAGG AAGCTGGTTT TTGCAGACCC CTCTCTGCGT
    CACAGTCTGG CTCAACAGAC AACTTTTCTC ATCACGCTGC TCAGAGCTTC TCTGATCATA
    AAGGAGAACC AGGGTGATGT GAGCGAGGCG GCTGCGTTGA TGTGCATTCT GCTGTTTGAT
    GAAATCGCAC GAATGGATGT TTGGACTGAC GAAGCTGGCT CAGACACAGG CCTGTCTTCC
    TTCTCACTTC CTGCAGCCAT TATACGGAGG TACAATTTGC CGTTTCAAGC TCCTTCACAT
    CATGCAGTGA GTCCATACTG TTCAGTTCTT CCTCCATACT CAGATCTCCT GAGTCTGAAG
    CCCGCCTGGG AAGCTCTGCA GATCACCTGG AACACAGCAT GGCATTCTGG GATTGATGGA
    TTGCTAGAGC ACCTGAGGGG TTTTAGAAGT GATGTGCCAG AGTTTCATAG TGATCTGGTG
    CTGTCTGAAT CTCAGGCTGT GGTGTTAAGA ATCAGCAACA TGAGGTCCGG TATAGAGGAC
    TGCATTCACA CGGTTTGTGA TGCGAGCTCC CACAGCACCG TCACTTTTGC CATCACTAGA
    ATGCATCTGT ATCTTCTTTT TGACTTCCTT GCACTCAAAT CCACCACGCA AAGCTGCAAG
    AACATTCTGC AAACCCTCAG CTGGCACAGT GCTGTAGAGA GATTTCTGGT TGTACGTCCA
    GCTTGCCATC AAGATGAGAG GCTCCTGGTG GACATTCTGT CCTTCCTCAA CACGTTCTTC
    AAGCAGAATC AATCTGAACT GGACCAGCAG GACCTTAAAT GGTTGTTGGA ATTACTCCTA
    AACCAGGAGT CGCACACACT GCTGAACTTG TTGCTGAGCG CTGATCCGCA GAACCACACA
    CACGTCCAGT CACAGGGGGA AGTGGACAAA CTCCAGGCCC ACCTCAGTCA CCGGCTACAG
    AGAGAACTGA CAGGCCTCTT CAACACATTG CTGCTTCTGA TGACATTCAC TTCTGACAGG
    ATGTGTGAGG TTTTGTCTGG ACCGTTTGCC TCTGAACTGT CTCTGCGTCT GCTGCAGTGT
    CTGCGCCTGT CTGATGCTCC GTGCTTTTAC GGCCTGCCGT CTCTAGAGCG CACACTCAGG
    ACCATGGCCC ACGTGACTGC CCTGCCCGGC TGGAGCACCC ACACTCCTAG CGTAGAGCCA
    AACACGCTCT GTCTCAAGTA CCTCAGCGGC CTTCTGGAAG TCATATCTTC ATTCTATGTC
    GATTTGGGAG GAAATTCAAT GTCCTTTATG GGCAAAGGAG TAACAAAAAA TGCAGTGATC
    TGCCTGCTTC ATTTGTCACA CGAGATGATG AAGGAAAGCA CAAATAAGGA CTGGATATCC
    TTGTGGTCTC TGGGGCGAGA GCAAAGTGCT GATGAGCAAA GTGCCGCTCA GCTTGGCTTG
    GCCTGGTTAA TTCCTCTGTG GGTGGATCGT GACCCTGAGG TCCGCTGTGC CAGTCTGGCA
    GTGGGTGCGG CTCTGAGTTC GCTGGAGAGA GGGTGTGTGG CTCTGTCTGC GAGCTGCCAG
    AACATTTCTG GCGGCCTGTG GGCCACAGTA CTCAACATCC TGCTGGAGAA ACAGGAGTGC
    AGTATGGTGC GCAGAGAGGC AGCGTTTATT TTGCAGAACC TCCTTGTGAT GCCAATGCCT
    GCAAATGCAG AAGAGGCCAA AGAGTGTGCC TGGCAGAGCC CCTGCGTGCA TGATGAGGAT
    TCAGGCCTGT GTCTGGTCGG GCTTCCTGCC CTGCAGGCTC TGTTGTACCA CTGCCAGTTT
    TTTGAGAGCG TGGGCCAGAT GGTGAAGAGC TGTTACACGG GCAGACATGC CTTCACCCTC
    AGCCCCCCTC CGCCGCCAGC CAATGTCACC CAGGATGCAG ATTTGAATGA TTCTCTGAAG
    CACTGGAGAG AGATGCTTGT TCCTCTAAAC CAGACTCATC CCTCTGGATC TATGTCCACC
    TCCAGTACTC TGATTCCATC AGGAAGCTGC AGTGGGACTC ATTCTCCAGT CCCGACCTCA
    GGTGTCTGCG CTAACCAGGA AGCTCCAGTT AACAGACTCA CTGCTCAGGG TCAGAGTGAC
    ACTGACAGCT CAGACTCTGC CCTCACTCAA GACTCACACC CCGGGGGGCG GAGCGAGCCT
    GTTGCTATAG TGACCCCTCA CCTGTTGCTG GCTGTTTGTG GTTTATTGGA CAACCTGCTG
    GCGGTGCTTC CGGAATTTAC TTTCTCTGCC CTCCAACAGA ACCAGATACT TCAGGGCATG
    GCCAGTGTGG TGGATGCGGC AGCCATATCT CAATGTGTCA ATGAGCTAAA AACACCTCTC
    CTGCCAGCTG CTATGGAAGA TGCCAGGAAC CAGGTGCTGA GTAGCTTGTA TTATGTGCAA
    AGTTTCTGTA AGCTCCTGCA GTCAGCAACG ATACACAGTG AGGATGTGCC GCTCCACAGA
    GAGTACTTCA AACTCCTGCT GAACAACCTC ATTTCCATCC TCACGCTGCC TGTCGCTGAC
    TTGGATGCGC AGACCCAGGG TGCAGTGCTC AACACCTGGT CGGATGTGTT GACGCTTCTG
    GCTACTCTGC TGCGCAGGAA CCAAGCTGCG GTCGGCCCAT CTGTCGCTGT GGCACTGGGG
    AAACACTGGC AGCGTTTTTC AGACACCCTC GGAGTGTGTT TGGACCAATC GGATGGTGAG
    TCTTCTCTCT ACATATCGAC TCTGCACTTT CTGTGTGTGC TGTTCTCAGA CGAGGCTAAG
    AGGAGAACTG GAGGACACAT GGAGGATCTG AGCAGCTCCT CCATTACTGC GGTCTTGGCT
    TCTGAGTCTG GAGAAGTCAT ATGTGAACTG TTACTAGAGC AATATGAGAA GATTTCATTC
    CAGGACCCTC TGAAAAAAAT GGTGGCCAAA GCACTTATGT CTCTCTTAGC CTGTTGCTCC
    AGTGCTCAGA GTTACGCATG TAAAGCTGGT GTGATTGACA GCTGTGTGGA GCGCATGAAG
    CACATTTACG CAGAGCTTCA CCTGGAGTCT GTGAAGACAG GCAGGAATGC TCACAGGAGG
    AAGGTGGAGA ACAGCATGAG AGAACTTAAG ATGATTCTAG AGATGTTGCG AAATTGCCTG
    TATCAACATA ATGAATGCAA AGCTGTGGCT ACAGATTTGC GTCTGGCTGT CGTGCTGTTG
    GCTTTTTGGC CCTGGCTCCT GCTTGATGAT CTCACCATGG CAGCGGCTCT GGAACTGCTC
    TGCGTCTACA CAGCGAACTG CACAGCGGCT TGCAGTGCTT TATGTTTCAG CAGCAGCAGT
    AGCAGTGTCA GCGTTGGCTC CGTTCAGCCT GTTCTTCCTA AGACTGCGGT CACAAACTCA
    CTGATGCATG CAGTGATGAA ACTGGCCTCC CAGGTTGCCC CTGACAACAG CAGCATCCAC
    AGCCTGGCTT TCTCACTGCT GGCCAACTTG GCCATCTCCA GGGATGGCAA AGGGCTGCTA
    CTGAAGTGTA ACTTTCTGCA GAACTTCCTG TCTCTCCCCA TGCCTAAAGC CAGTGGTCGA
    GGCGGTGCTC CAGCTGTGGA GACATTGTCT CTCTGGCTGC GGCTGCTGCT GAACGTGTCA
    TTTGGAGAGG ACGGACAGCA GATGATCCTC AGGCTCAGGG GAGCTCTGGA GCTGCTGGTG
    GAACTGACTC AGTCCAAACA CTCCAGCGCT AAACCCACCG TCTTGCTCAT CCTCCACAAC
    ATCTGCTTCT GCTCCGCTAA CAAGCCCAAA GTCTTAGCCA ACGATAAAGC TGTTGAACTG
    CTAGTCTCTT GTTTAAGCAG TAGCTCATCA GATATCCGCA ACATAGGAGC TTCTGCACTT
    TGGGCTTTGT TGCACAACAA TCAGAAGGCA AAAGCCACTT TGAAGTGTCC TTCCATCAGA
    CTGAAAGTTG AAGAGGCATT CGTGTCCGCA AGGAAAGATG AAGAGCAGAG CAATGACAAA
    GCTGTGAACA TCTATCATCT GAAGTGCCTC CGAAATCTTT CCAACATTTT CAACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209456.1
    CDS64..5403
    Translation

    Target ORF information:

    RefSeq Version XM_017353967.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio rotatin (rttn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTTCCT CAGAACATCC AGAATCGTCT CTGATCCACC ACAGAATGGT CTTCACAGGC 
    ACTGCCGTTT TCACAATACG TCTCCTGCAA ACCTTGTTAC CTGTGGAGAA GGCTGCGGAT
    AATCTTCCGG AAAGTACCGT GGCTGCCATT TTCCTGTTGT GTCTTGATAT ATCCTTCAGC
    GTGGCGTTCC CCGTCATCCA TGAGTCAGCC GTGGCGTATC TAGAGCAGGC TTGCTTTGAA
    AGATACAGCC TGTATCGTAG AGTGTCCCGT ATTTCTCATT GCATGGAGGC GACATGCACC
    TTTTTAAAGG ATGCACAGGC TGAGGGCGAT AAGAACTGGC ATGAGCTTTT GGAGCTTGCT
    GACCAAGGAG TAGATGCTTT TCCATATCAT CAGCACCTAC CGGTGGTGAA GGAATGTGTC
    CAGATTTGCT CCTACCTGTG GAAATTTGCA CAAGCAAGCC CGCTTCTGCA GTCGGAGAGT
    CAGAAGATAT TTCTGAAGCT TTTGTCTCAT CCTCTGTCCC CAGTGAAGGC AGAAACCTAC
    TCCTGCACTC TGAGCATTTT GAAGGAGTGT TTGGGAGTTC AGAACGTGGC CAAACCTGCC
    TCATCCATCT GCGCCGGAGT GCATTTCTTG CTTCACTCCC GTGTCCTGTA TGAGATCAGT
    GTGTTTGGCC TTCAAGACCA AGCAACAGAG GTGAGTGCCG CTGCCAAGGA TATTCTGCTT
    TATCTGCTGA AGGGACGATT GATGATGACA GCTGCAAGTT GGAGGAGATT TAATGAGGCA
    CTTTACCCGG TCATTCCTAT ATTACAGAGC TACGCAGGCA CTGAAGACGC CCTGGGAAAC
    TGTATCCTGC TGCTAAGTGA AGCATCTGCT GAGAAAGGAG ATGAGATGCG TTCCCCGACT
    GCTAGGCTAC GGGCAGCCCT CCGGCTTCTG TTCACCAAAC AGCACACCGT GAGGAAAATG
    GCAGTCCAAC ATCTGCTTCC ACATCTGACC AGCGCAGAGG GGGCCCAAAA CTCCAGACCA
    ACCCTGGACA GCTCCACTCT ATCATGTTTA CCAAATCTCT ACAATCTTAA TAAAAGTGTG
    GACATCAAGT TCAGCAGTTC AGACAAGTCA TATGTAAAGG TGGAGTCTGT AAATAAGTTG
    TACAATATTC TCGCATCGGA AACGGTAGAT CTTGTTTTAC GGAAATCTGC AGCTGAACAG
    TTGGCTATTG TTCTGCAAGA TTCATCAATG CACGCGGTGC TGAAGGCCTT GGGTTTATCA
    GACAAGCTCA CGTCTTTCAT CACAGAGTGT GTCAACAGCA ATGTGCCGAG CATGGATTGC
    TTGTTGGAGT CCAGTGCATG CATGCTGAGG AAGCTGGTTT TTGCAGACCC CTCTCTGCGT
    CACAGTCTGG CTCAACAGAC AACTTTTCTC ATCACGCTGC TCAGAGCTTC TCTGATCATA
    AAGGAGAACC AGGGTGATGT GAGCGAGGCG GCTGCGTTGA TGTGCATTCT GCTGTTTGAT
    GAAATCGCAC GAATGGATGT TTGGACTGAC GAAGCTGGCT CAGACACAGG CCTGTCTTCC
    TTCTCACTTC CTGCAGCCAT TATACGGAGG TACAATTTGC CGTTTCAAGC TCCTTCACAT
    CATGCAGTGA GTCCATACTG TTCAGTTCTT CCTCCATACT CAGATCTCCT GAGTCTGAAG
    CCCGCCTGGG AAGCTCTGCA GATCACCTGG AACACAGCAT GGCATTCTGG GATTGATGGA
    TTGCTAGAGC ACCTGAGGGG TTTTAGAAGT GATGTGCCAG AGTTTCATAG TGATCTGGTG
    CTGTCTGAAT CTCAGGCTGT GGTGTTAAGA ATCAGCAACA TGAGGTCCGG TATAGAGGAC
    TGCATTCACA CGGTTTGTGA TGCGAGCTCC CACAGCACCG TCACTTTTGC CATCACTAGA
    ATGCATCTGT ATCTTCTTTT TGACTTCCTT GCACTCAAAT CCACCACGCA AAGCTGCAAG
    AACATTCTGC AAACCCTCAG CTGGCACAGT GCTGTAGAGA GATTTCTGGT TGTACGTCCA
    GCTTGCCATC AAGATGAGAG GCTCCTGGTG GACATTCTGT CCTTCCTCAA CACGTTCTTC
    AAGCAGAATC AATCTGAACT GGACCAGCAG GACCTTAAAT GGTTGTTGGA ATTACTCCTA
    AACCAGGAGT CGCACACACT GCTGAACTTG TTGCTGAGCG CTGATCCGCA GAACCACACA
    CACGTCCAGT CACAGGGGGA AGTGGACAAA CTCCAGGCCC ACCTCAGTCA CCGGCTACAG
    AGAGAACTGA CAGGCCTCTT CAACACATTG CTGCTTCTGA TGACATTCAC TTCTGACAGG
    ATGTGTGAGG TTTTGTCTGG ACCGTTTGCC TCTGAACTGT CTCTGCGTCT GCTGCAGTGT
    CTGCGCCTGT CTGATGCTCC GTGCTTTTAC GGCCTGCCGT CTCTAGAGCG CACACTCAGG
    ACCATGGCCC ACGTGACTGC CCTGCCCGGC TGGAGCACCC ACACTCCTAG CGTAGAGCCA
    AACACGCTCT GTCTCAAGTA CCTCAGCGGC CTTCTGGAAG TCATATCTTC ATTCTATGTC
    GATTTGGGAG GAAATTCAAT GTCCTTTATG GGCAAAGGAG TAACAAAAAA TGCAGTGATC
    TGCCTGCTTC ATTTGTCACA CGAGATGATG AAGGAAAGCA CAAATAAGGA CTGGATATCC
    TTGTGGTCTC TGGGGCGAGA GCAAAGTGCT GATGAGCAAA GTGCCGCTCA GCTTGGCTTG
    GCCTGGTTAA TTCCTCTGTG GGTGGATCGT GACCCTGAGG TCCGCTGTGC CAGTCTGGCA
    GTGGGTGCGG CTCTGAGTTC GCTGGAGAGA GGGTGTGTGG CTCTGTCTGC GAGCTGCCAG
    AACATTTCTG GCGGCCTGTG GGCCACAGTA CTCAACATCC TGCTGGAGAA ACAGGAGTGC
    AGTATGGTGC GCAGAGAGGC AGCGTTTATT TTGCAGAACC TCCTTGTGAT GCCAATGCCT
    GCAAATGCAG AAGAGGCCAA AGAGTGTGCC TGGCAGAGCC CCTGCGTGCA TGATGAGGAT
    TCAGGCCTGT GTCTGGTCGG GCTTCCTGCC CTGCAGGCTC TGTTGTACCA CTGCCAGTTT
    TTTGAGAGCG TGGGCCAGAT GGTGAAGAGC TGTTACACGG GCAGACATGC CTTCACCCTC
    AGCCCCCCTC CGCCGCCAGC CAATGTCACC CAGGATGCAG ATTTGAATGA TTCTCTGAAG
    CACTGGAGAG AGATGCTTGT TCCTCTAAAC CAGACTCATC CCTCTGGATC TATGTCCACC
    TCCAGTACTC TGATTCCATC AGGAAGCTGC AGTGGGACTC ATTCTCCAGT CCCGACCTCA
    GGTGTCTGCG CTAACCAGGA AGCTCCAGTT AACAGACTCA CTGCTCAGGG TCAGAGTGAC
    ACTGACAGCT CAGACTCTGC CCTCACTCAA GACTCACACC CCGGGGGGCG GAGCGAGCCT
    GTTGCTATAG TGACCCCTCA CCTGTTGCTG GCTGTTTGTG GTTTATTGGA CAACCTGCTG
    GCGGTGCTTC CGGAATTTAC TTTCTCTGCC CTCCAACAGA ACCAGATACT TCAGGGCATG
    GCCAGTGTGG TGGATGCGGC AGCCATATCT CAATGTGTCA ATGAGCTAAA AACACCTCTC
    CTGCCAGCTG CTATGGAAGA TGCCAGGAAC CAGGTGCTGA GTAGCTTGTA TTATGTGCAA
    AGTTTCTGTA AGCTCCTGCA GTCAGCAACG ATACACAGTG AGGATGTGCC GCTCCACAGA
    GAGTACTTCA AACTCCTGCT GAACAACCTC ATTTCCATCC TCACGCTGCC TGTCGCTGAC
    TTGGATGCGC AGACCCAGGG TGCAGTGCTC AACACCTGGT CGGATGTGTT GACGCTTCTG
    GCTACTCTGC TGCGCAGGAA CCAAGCTGCG GTCGGCCCAT CTGTCGCTGT GGCACTGGGG
    AAACACTGGC AGCGTTTTTC AGACACCCTC GGAGTGTGTT TGGACCAATC GGATGGTGAG
    TCTTCTCTCT ACATATCGAC TCTGCACTTT CTGTGTGTGC TGTTCTCAGA CGAGGCTAAG
    AGGAGAACTG GAGGACACAT GGAGGATCTG AGCAGCTCCT CCATTACTGC GGTCTTGGCT
    TCTGAGTCTG GAGAAGTCAT ATGTGAACTG TTACTAGAGC AATATGAGAA GATTTCATTC
    CAGGACCCTC TGAAAAAAAT GGTGGCCAAA GCACTTATGT CTCTCTTAGC CTGTTGCTCC
    AGTGCTCAGA GTTACGCATG TAAAGCTGGT GTGATTGACA GCTGTGTGGA GCGCATGAAG
    CACATTTACG CAGAGCTTCA CCTGGAGTCT GTGAAGACAG GCAGGAATGC TCACAGGAGG
    AAGGTGGAGA ACAGCATGAG AGAACTTAAG ATGATTCTAG AGATGTTGCG AAATTGCCTG
    TATCAACATA ATGAATGCAA AGCTGTGGCT ACAGATTTGC GTCTGGCTGT CGTGCTGTTG
    GCTTTTTGGC CCTGGCTCCT GCTTGATGAT CTCACCATGG CAGCGGCTCT GGAACTGCTC
    TGCGTCTACA CAGCGAACTG CACAGCGGCT TGCAGTGCTT TATGTTTCAG CAGCAGCAGT
    AGCAGTGTCA GCGTTGGCTC CGTTCAGCCT GTTCTTCCTA AGACTGCGGT CACAAACTCA
    CTGATGCATG CAGTGATGAA ACTGGCCTCC CAGGTTGCCC CTGACAACAG CAGCATCCAC
    AGCCTGGCTT TCTCACTGCT GGCCAACTTG GCCATCTCCA GGGATGGCAA AGGGCTGCTA
    CTGAAGTGTA ACTTTCTGCA GAACTTCCTG TCTCTCCCCA TGCCTAAAGC CAGTGGTCGA
    GGCGGTGCTC CAGCTGTGGA GACATTGTCT CTCTGGCTGC GGCTGCTGCT GAACGTGTCA
    TTTGGAGAGG ACGGACAGCA GATGATCCTC AGGCTCAGGG GAGCTCTGGA GCTGCTGGTG
    GAACTGACTC AGTCCAAACA CTCCAGCGCT AAACCCACCG TCTTGCTCAT CCTCCACAAC
    ATCTGCTTCT GCTCCGCTAA CAAGCCCAAA GTCTTAGCCA ACGATAAAGC TGTTGAACTG
    CTAGTCTCTT GTTTAAGCAG TAGCTCATCA GATATCCGCA ACATAGGAGC TTCTGCACTT
    TGGGCTTTGT TGCACAACAA TCAGAAGGCA AAAGCCACTT TGAAGTGTCC TTCCATCAGA
    CTGAAAGTTG AAGAGGCATT CGTGTCCGCA AGGAAAGATG AAGAGCAGAG CAATGACAAA
    GCTGTGAACA TCTATCATCT GAAGTGCCTC CGAAATCTTT CCAACATTTT CAACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28452
    Clone ID Related Accession (Same CDS sequence) XM_021470297.1
    Accession Version XM_021470297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rotatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGTCA TAGAGGGGCT
    CCCCTTAGGC CAGCCCAGCA GTCTCCCCTC CCGTCTGCCC ACCCAGAGCT CCCCCCGGAG
    ATGATGATGG AAACGGAGGA TGCGCTGGAG CTCCAGCTGC AGCAGTGGAG CCTGGCTAGA
    TTCGCCGTAG CAGCCGTAGA GCACGCTCTG CCTCTCATCA GGACAGAAAG CCTGAAGGTT
    TTCCAGCGGG TGCTAGAGCT GCTGGCGGAG GCGCAGGGGC TGCTGGGAGA CAGCGTAGCC
    CCTGAGGTGT GGGACGAGCA CAGCCTGACT GCGACAGAGC TGAGAGAGAA ATTACAGGCA
    TGTATAGAGA TTTTGGGAGA TGTTATTTGC TACCACCATG GCATGACTTC CTCAGAACAT
    CCAGAATCGT CTCTGATCCA CCACAGAATG GTCTTCACAG GCACTGCCGT TTTCACAATA
    CGTCTCCTGC AAACCTTGTT ACCTGTGGAG AAGGCTGCGG ATAATCTTCC GGAAAGTACC
    GTGGCTGCCA TTTTCCTGTT GTGTCTTGAT ATATCCTTCA GCGTGGCGTT CCCCGTCATC
    CATGAGTCAG CCGTGGCGTA TCTAGAGCAG GCTTGCTTTG AAAGATACAG CCTGTATCGT
    AGAGTGTCCC GTATTTCTCA TTGCATGGAG GCGACATGCA CCTTTTTAAA GGATGCACAG
    GCTGAGGGCG ATAAGAACTG GCATGAGCTT TTGGAGCTTG CTGACCAAGG AGTAGATGCT
    TTTCCATATC ATCAGCACCT ACCGGTGGTG AAGGAATGTG TCCAGATTTG CTCCTACCTG
    TGGAAATTTG CACAAGCAAG CCCGCTTCTG CAGTCGGAGA GTCAGAAGAT ATTTCTGAAG
    CTTTTGTCTC ATCCTCTGTC CCCAGTGAAG GCAGAAACCT ACTCCTGCAC TCTGAGCATT
    TTGAAGGAGT GTTTGGGAGT TCAGAACGTG GCCAAACCTG CCTCATCCAT CTGCGCCGGA
    GTGCATTTCT TGCTTCACTC CCGTGTCCTG TATGAGATCA GTGTGTTTGG CCTTCAAGAC
    CAAGCAACAG AGGTGAGTGC CGCTGCCAAG GATATTCTGC TTTATCTGCT GAAGGGACGA
    TTGATGATGA CAGCTGCAAG TTGGAGGAGA TTTAATGAGG CACTTTACCC GGTCATTCCT
    ATATTACAGA GCTACGCAGG CACTGAAGAC GCCCTGGGAA ACTGTATCCT GCTGCTAAGT
    GAAGCATCTG CTGAGAAAGG AGATGAGATG CGTTCCCCGA CTGCTAGGCT ACGGGCAGCC
    CTCCGGCTTC TGTTCACCAA ACAGCACACC GTGAGGAAAA TGGCAGTCCA ACATCTGCTT
    CCACATCTGA CCAGCGCAGA GGGGGCCCAA AACTCCAGAC CAACCCTGGA CAGCTCCACT
    CTATCATGTT TACCAAATCT CTACAATCTT AATAAAAGTG TGGACATCAA GTTCAGCAGT
    TCAGACAAGT CATATGTAAA GGTGGAGTCT GTAAATAAGT TGTACAATAT TCTCGCATCG
    GAAACGGTAG ATCTTGTTTT ACGGAAATCT GCAGCTGAAC AGTTGGCTAT TGTTCTGCAA
    GATTCATCAA TGCACGCGGT GCTGAAGGCC TTGGGTTTAT CAGACAAGCT CACGTCTTTC
    ATCACAGAGT GTGTCAACAG CAATGTGCCG AGCATGGATT GCTTGTTGGA GTCCAGTGCA
    TGCATGCTGA GGAAGCTGGT TTTTGCAGAC CCCTCTCTGC GTCACAGTCT GGCTCAACAG
    ACAACTTTTC TCATCACGCT GCTCAGAGCT TCTCTGATCA TAAAGGAGAA CCAGGGTGAT
    GTGAGCGAGG CGGCTGCGTT GATGTGCATT CTGCTGTTTG ATGAAATCGC ACGAATGGAT
    GTTTGGACTG ACGAAGCTGG CTCAGACACA GGCCTGTCTT CCTTCTCACT TCCTGCAGCC
    ATTATACGGA GGTACAATTT GCCGTTTCAA GCTCCTTCAC ATCATGCAGT GAGTCCATAC
    TGTTCAGTTC TTCCTCCATA CTCAGATCTC CTGAGTCTGA AGCCCGCCTG GGAAGCTCTG
    CAGATCACCT GGAACACAGC ATGGCATTCT GGGATTGATG GATTGCTAGA GCACCTGAGG
    GGTTTTAGAA GTGATGTGCC AGAGTTTCAT AGTGATCTGG TGCTGTCTGA ATCTCAGGCT
    GTGGTGTTAA GAATCAGCAA CATGAGGTCC GGTATAGAGG ACTGCATTCA CACGGTTTGT
    GATGCGAGCT CCCACAGCAC CGTCACTTTT GCCATCACTA GAATGCATCT GTATCTTCTT
    TTTGACTTCC TTGCACTCAA ATCCACCACG CAAAGCTGCA AGAACATTCT GCAAACCCTC
    AGCTGGCACA GTGCTGTAGA GAGATTTCTG GTTGTACGTC CAGCTTGCCA TCAAGATGAG
    AGGCTCCTGG TGGACATTCT GTCCTTCCTC AACACGTTCT TCAAGCAGAA TCAATCTGAA
    CTGGACCAGC AGGACCTTAA ATGGTTGTTG GAATTACTCC TAAACCAGGA GTCGCACACA
    CTGCTGAACT TGTTGCTGAG CGCTGATCCG CAGAACCACA CACACGTCCA GTCACAGGGG
    GAAGTGGACA AACTCCAGGC CCACCTCAGT CACCGGCTAC AGAGAGAACT GACAGGCCTC
    TTCAACACAT TGCTGCTTCT GATGACATTC ACTTCTGACA GGATGTGTGA GGTTTTGTCT
    GGACCGTTTG CCTCTGAACT GTCTCTGCGT CTGCTGCAGT GTCTGCGCCT GTCTGATGCT
    CCGTGCTTTT ACGGCCTGCC GTCTCTAGAG CGCACACTCA GGACCATGGC CCACGTGACT
    GCCCTGCCCG GCTGGAGCAC CCACACTCCT AGCGTAGAGC CAAACACGCT CTGTCTCAAG
    TACCTCAGCG GCCTTCTGGA AGTCATATCT TCATTCTATG TCGATTTGGG AGGAAATTCA
    ATGTCCTTTA TGGGCAAAGG AGTAACAAAA AATGCAGTGA TCTGCCTGCT TCATTTGTCA
    CACGAGATGA TGAAGGAAAG CACAAATAAG GACTGGATAT CCTTGTGGTC TCTGGGGCGA
    GAGCAAAGTG CTGATGAGCA AAGTGCCGCT CAGCTTGGCT TGGCCTGGTT AATTCCTCTG
    TGGGTGGATC GTGACCCTGA GGTCCGCTGT GCCAGTCTGG CAGTGGGTGC GGCTCTGAGT
    TCGCTGGAGA GAGGGTGTGT GGCTCTGTCT GCGAGCTGCC AGAACATTTC TGGCGGCCTG
    TGGGCCACAG TACTCAACAT CCTGCTGGAG AAACAGGAGT GCAGTATGGT GCGCAGAGAG
    GCAGCGTTTA TTTTGCAGAA CCTCCTTGTG ATGCCAATGC CTGCAAATGC AGAAGAGGCC
    AAAGAGTGTG CCTGGCAGAG CCCCTGCGTG CATGATGAGG ATTCAGGCCT GTGTCTGGTC
    GGGCTTCCTG CCCTGCAGGC TCTGTTGTAC CACTGCCAGT TTTTTGAGAG CGTGGGCCAG
    ATGGTGAAGA GCTGTTACAC GGGCAGACAT GCCTTCACCC TCAGCCCCCC TCCGCCGCCA
    GCCAATGTCA CCCAGGATGC AGATTTGAAT GATTCTCTGA AGCACTGGAG AGAGATGCTT
    GTTCCTCTAA ACCAGACTCA TCCCTCTGGA TCTATGTCCA CCTCCAGTAC TCTGATTCCA
    TCAGGAAGCT GCAGTGGGAC TCATTCTCCA GTCCCGACCT CAGGTGTCTG CGCTAACCAG
    GAAGCTCCAG TTAACAGACT CACTGCTCAG GGTCAGAGTG ACACTGACAG CTCAGACTCT
    GCCCTCACTC AAGACTCACA CCCCGGGGGG CGGAGCGAGC CTGTTGCTAT AGTGACCCCT
    CACCTGTTGC TGGCTGTTTG TGGTTTATTG GACAACCTGC TGGCGGTGCT TCCGGAATTT
    ACTTTCTCTG CCCTCCAACA GAACCAGATA CTTCAGGGCA TGGCCAGTGT GGTGGATGCG
    GCAGCCATAT CTCAATGTGT CAATGAGCTA AAAACACCTC TCCTGCCAGC TGCTATGGAA
    GATGCCAGGA ACCAGGTGCT GAGTAGCTTG TATTATGTGC AAAGTTTCTG TAAGCTCCTG
    CAGTCAGCAA CGATACACAG TGAGGATGTG CCGCTCCACA GAGAGTACTT CAAACTCCTG
    CTGAACAACC TCATTTCCAT CCTCACGCTG CCTGTCGCTG ACTTGGATGC GCAGACCCAG
    GGTGCAGTGC TCAACACCTG GTCGGATGTG TTGACGCTTC TGGCTACTCT GCTGCGCAGG
    AACCAAGCTG CGGTCGGCCC ATCTGTCGCT GTGGCACTGG GGAAACACTG GCAGCGTTTT
    TCAGACACCC TCGGAGTGTG TTTGGACCAA TCGGATGGTG AGTCTTCTCT CTACATATCG
    ACTCTGCACT TTCTGTGTGT GCTGTTCTCA GACGAGGCTA AGAGGAGAAC TGGAGGACAC
    ATGGAGGATC TGAGCAGCTC CTCCATTACT GCGGTCTTGG CTTCTGAGTC TGGAGAAGTC
    ATATGTGAAC TGTTACTAGA GCAATATGAG AAGATTTCAT TCCAGGACCC TCTGAAAAAA
    ATGGTGGCCA AAGCACTTAT GTCTCTCTTA GCCTGTTGCT CCAGTGCTCA GAGTTACGCA
    TGTAAAGCTG GTGTGATTGA CAGCTGTGTG GAGCGCATGA AGCACATTTA CGCAGAGCTT
    CACCTGGAGT CTGTGAAGAC AGGCAGGAAT GCTCACAGGA GGAAGCAGGT GGAGAACAGC
    ATGAGAGAAC TTAAGATGAT TCTAGAGATG TTGCGAAATT GCCTGTATCA ACATAATGAA
    TGCAAAGCTG TGGCTACAGA TTTGCGTCTG GCTGTCGTGC TGTTGGCTTT TTGGCCCTGG
    CTCCTGCTTG ATGATCTCAC CATGGCAGCG GCTCTGGAAC TGCTCTGCGT CTACACAGCG
    AACTGCACAG CGGCTTGCAG TGCTTTATGT TTCAGCAGCA GCAGTAGCAG TGTCAGCGTT
    GGCTCCGTTC AGCCTGTTCT TCCTAAGACT GCGGTCACAA ACTCACTGAT GCATGCAGTG
    ATGAAACTGG CCTCCCAGGT TGCCCCTGAC AACAGCAGCA TCCACAGCCT GGCTTTCTCA
    CTGCTGGCCA ACTTGGCCAT CTCCAGGGAT GGCAAAGGGC TGCTACTGAA GTGTAACTTT
    CTGCAGAACT TCCTGTCTCT CCCCATGCCT AAAGCCAGTG GTCGAGGCGG TGCTCCAGCT
    GTGGAGACAT TGTCTCTCTG GCTGCGGCTG CTGCTGAACG TGTCATTTGG AGAGGACGGA
    CAGCAGATGA TCCTCAGGCT CAGGGGAGCT CTGGAGCTGC TGGTGGAACT GACTCAGTCC
    AAACACTCCA GCGCTAAACC CACCGTCTTG CTCATCCTCC ACAACATCTG CTTCTGCTCC
    GCTAACAAGC CCAAAGTCTT AGCCAACGAT AAAGCTGTTG AACTGCTAGT CTCTTGTTTA
    AGCAGTAGCT CATCAGATAT CCGCAACATA GGAGCTTCTG CACTTTGGGC TTTGTTGCAC
    AACAATCAGA AGGCAAAAGC CACTTTGAAG TGTCCTTCCA TCAGACTGAA AGTTGAAGAG
    GCATTCGTGT CCGCAAGGAA AGATGAAGAG CAGAGCAATG ACAAAGCTGT GAACATCTAT
    CATCTGAAGT GCCTCCGAAA TCTTTCCAAC ATTTTCAACT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325972.1
    CDS91..6795
    Translation

    Target ORF information:

    RefSeq Version XM_021470297.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio rotatin (rttn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGTCA TAGAGGGGCT
    CCCCTTAGGC CAGCCCAGCA GTCTCCCCTC CCGTCTGCCC ACCCAGAGCT CCCCCCGGAG
    ATGATGATGG AAACGGAGGA TGCGCTGGAG CTCCAGCTGC AGCAGTGGAG CCTGGCTAGA
    TTCGCCGTAG CAGCCGTAGA GCACGCTCTG CCTCTCATCA GGACAGAAAG CCTGAAGGTT
    TTCCAGCGGG TGCTAGAGCT GCTGGCGGAG GCGCAGGGGC TGCTGGGAGA CAGCGTAGCC
    CCTGAGGTGT GGGACGAGCA CAGCCTGACT GCGACAGAGC TGAGAGAGAA ATTACAGGCA
    TGTATAGAGA TTTTGGGAGA TGTTATTTGC TACCACCATG GCATGACTTC CTCAGAACAT
    CCAGAATCGT CTCTGATCCA CCACAGAATG GTCTTCACAG GCACTGCCGT TTTCACAATA
    CGTCTCCTGC AAACCTTGTT ACCTGTGGAG AAGGCTGCGG ATAATCTTCC GGAAAGTACC
    GTGGCTGCCA TTTTCCTGTT GTGTCTTGAT ATATCCTTCA GCGTGGCGTT CCCCGTCATC
    CATGAGTCAG CCGTGGCGTA TCTAGAGCAG GCTTGCTTTG AAAGATACAG CCTGTATCGT
    AGAGTGTCCC GTATTTCTCA TTGCATGGAG GCGACATGCA CCTTTTTAAA GGATGCACAG
    GCTGAGGGCG ATAAGAACTG GCATGAGCTT TTGGAGCTTG CTGACCAAGG AGTAGATGCT
    TTTCCATATC ATCAGCACCT ACCGGTGGTG AAGGAATGTG TCCAGATTTG CTCCTACCTG
    TGGAAATTTG CACAAGCAAG CCCGCTTCTG CAGTCGGAGA GTCAGAAGAT ATTTCTGAAG
    CTTTTGTCTC ATCCTCTGTC CCCAGTGAAG GCAGAAACCT ACTCCTGCAC TCTGAGCATT
    TTGAAGGAGT GTTTGGGAGT TCAGAACGTG GCCAAACCTG CCTCATCCAT CTGCGCCGGA
    GTGCATTTCT TGCTTCACTC CCGTGTCCTG TATGAGATCA GTGTGTTTGG CCTTCAAGAC
    CAAGCAACAG AGGTGAGTGC CGCTGCCAAG GATATTCTGC TTTATCTGCT GAAGGGACGA
    TTGATGATGA CAGCTGCAAG TTGGAGGAGA TTTAATGAGG CACTTTACCC GGTCATTCCT
    ATATTACAGA GCTACGCAGG CACTGAAGAC GCCCTGGGAA ACTGTATCCT GCTGCTAAGT
    GAAGCATCTG CTGAGAAAGG AGATGAGATG CGTTCCCCGA CTGCTAGGCT ACGGGCAGCC
    CTCCGGCTTC TGTTCACCAA ACAGCACACC GTGAGGAAAA TGGCAGTCCA ACATCTGCTT
    CCACATCTGA CCAGCGCAGA GGGGGCCCAA AACTCCAGAC CAACCCTGGA CAGCTCCACT
    CTATCATGTT TACCAAATCT CTACAATCTT AATAAAAGTG TGGACATCAA GTTCAGCAGT
    TCAGACAAGT CATATGTAAA GGTGGAGTCT GTAAATAAGT TGTACAATAT TCTCGCATCG
    GAAACGGTAG ATCTTGTTTT ACGGAAATCT GCAGCTGAAC AGTTGGCTAT TGTTCTGCAA
    GATTCATCAA TGCACGCGGT GCTGAAGGCC TTGGGTTTAT CAGACAAGCT CACGTCTTTC
    ATCACAGAGT GTGTCAACAG CAATGTGCCG AGCATGGATT GCTTGTTGGA GTCCAGTGCA
    TGCATGCTGA GGAAGCTGGT TTTTGCAGAC CCCTCTCTGC GTCACAGTCT GGCTCAACAG
    ACAACTTTTC TCATCACGCT GCTCAGAGCT TCTCTGATCA TAAAGGAGAA CCAGGGTGAT
    GTGAGCGAGG CGGCTGCGTT GATGTGCATT CTGCTGTTTG ATGAAATCGC ACGAATGGAT
    GTTTGGACTG ACGAAGCTGG CTCAGACACA GGCCTGTCTT CCTTCTCACT TCCTGCAGCC
    ATTATACGGA GGTACAATTT GCCGTTTCAA GCTCCTTCAC ATCATGCAGT GAGTCCATAC
    TGTTCAGTTC TTCCTCCATA CTCAGATCTC CTGAGTCTGA AGCCCGCCTG GGAAGCTCTG
    CAGATCACCT GGAACACAGC ATGGCATTCT GGGATTGATG GATTGCTAGA GCACCTGAGG
    GGTTTTAGAA GTGATGTGCC AGAGTTTCAT AGTGATCTGG TGCTGTCTGA ATCTCAGGCT
    GTGGTGTTAA GAATCAGCAA CATGAGGTCC GGTATAGAGG ACTGCATTCA CACGGTTTGT
    GATGCGAGCT CCCACAGCAC CGTCACTTTT GCCATCACTA GAATGCATCT GTATCTTCTT
    TTTGACTTCC TTGCACTCAA ATCCACCACG CAAAGCTGCA AGAACATTCT GCAAACCCTC
    AGCTGGCACA GTGCTGTAGA GAGATTTCTG GTTGTACGTC CAGCTTGCCA TCAAGATGAG
    AGGCTCCTGG TGGACATTCT GTCCTTCCTC AACACGTTCT TCAAGCAGAA TCAATCTGAA
    CTGGACCAGC AGGACCTTAA ATGGTTGTTG GAATTACTCC TAAACCAGGA GTCGCACACA
    CTGCTGAACT TGTTGCTGAG CGCTGATCCG CAGAACCACA CACACGTCCA GTCACAGGGG
    GAAGTGGACA AACTCCAGGC CCACCTCAGT CACCGGCTAC AGAGAGAACT GACAGGCCTC
    TTCAACACAT TGCTGCTTCT GATGACATTC ACTTCTGACA GGATGTGTGA GGTTTTGTCT
    GGACCGTTTG CCTCTGAACT GTCTCTGCGT CTGCTGCAGT GTCTGCGCCT GTCTGATGCT
    CCGTGCTTTT ACGGCCTGCC GTCTCTAGAG CGCACACTCA GGACCATGGC CCACGTGACT
    GCCCTGCCCG GCTGGAGCAC CCACACTCCT AGCGTAGAGC CAAACACGCT CTGTCTCAAG
    TACCTCAGCG GCCTTCTGGA AGTCATATCT TCATTCTATG TCGATTTGGG AGGAAATTCA
    ATGTCCTTTA TGGGCAAAGG AGTAACAAAA AATGCAGTGA TCTGCCTGCT TCATTTGTCA
    CACGAGATGA TGAAGGAAAG CACAAATAAG GACTGGATAT CCTTGTGGTC TCTGGGGCGA
    GAGCAAAGTG CTGATGAGCA AAGTGCCGCT CAGCTTGGCT TGGCCTGGTT AATTCCTCTG
    TGGGTGGATC GTGACCCTGA GGTCCGCTGT GCCAGTCTGG CAGTGGGTGC GGCTCTGAGT
    TCGCTGGAGA GAGGGTGTGT GGCTCTGTCT GCGAGCTGCC AGAACATTTC TGGCGGCCTG
    TGGGCCACAG TACTCAACAT CCTGCTGGAG AAACAGGAGT GCAGTATGGT GCGCAGAGAG
    GCAGCGTTTA TTTTGCAGAA CCTCCTTGTG ATGCCAATGC CTGCAAATGC AGAAGAGGCC
    AAAGAGTGTG CCTGGCAGAG CCCCTGCGTG CATGATGAGG ATTCAGGCCT GTGTCTGGTC
    GGGCTTCCTG CCCTGCAGGC TCTGTTGTAC CACTGCCAGT TTTTTGAGAG CGTGGGCCAG
    ATGGTGAAGA GCTGTTACAC GGGCAGACAT GCCTTCACCC TCAGCCCCCC TCCGCCGCCA
    GCCAATGTCA CCCAGGATGC AGATTTGAAT GATTCTCTGA AGCACTGGAG AGAGATGCTT
    GTTCCTCTAA ACCAGACTCA TCCCTCTGGA TCTATGTCCA CCTCCAGTAC TCTGATTCCA
    TCAGGAAGCT GCAGTGGGAC TCATTCTCCA GTCCCGACCT CAGGTGTCTG CGCTAACCAG
    GAAGCTCCAG TTAACAGACT CACTGCTCAG GGTCAGAGTG ACACTGACAG CTCAGACTCT
    GCCCTCACTC AAGACTCACA CCCCGGGGGG CGGAGCGAGC CTGTTGCTAT AGTGACCCCT
    CACCTGTTGC TGGCTGTTTG TGGTTTATTG GACAACCTGC TGGCGGTGCT TCCGGAATTT
    ACTTTCTCTG CCCTCCAACA GAACCAGATA CTTCAGGGCA TGGCCAGTGT GGTGGATGCG
    GCAGCCATAT CTCAATGTGT CAATGAGCTA AAAACACCTC TCCTGCCAGC TGCTATGGAA
    GATGCCAGGA ACCAGGTGCT GAGTAGCTTG TATTATGTGC AAAGTTTCTG TAAGCTCCTG
    CAGTCAGCAA CGATACACAG TGAGGATGTG CCGCTCCACA GAGAGTACTT CAAACTCCTG
    CTGAACAACC TCATTTCCAT CCTCACGCTG CCTGTCGCTG ACTTGGATGC GCAGACCCAG
    GGTGCAGTGC TCAACACCTG GTCGGATGTG TTGACGCTTC TGGCTACTCT GCTGCGCAGG
    AACCAAGCTG CGGTCGGCCC ATCTGTCGCT GTGGCACTGG GGAAACACTG GCAGCGTTTT
    TCAGACACCC TCGGAGTGTG TTTGGACCAA TCGGATGGTG AGTCTTCTCT CTACATATCG
    ACTCTGCACT TTCTGTGTGT GCTGTTCTCA GACGAGGCTA AGAGGAGAAC TGGAGGACAC
    ATGGAGGATC TGAGCAGCTC CTCCATTACT GCGGTCTTGG CTTCTGAGTC TGGAGAAGTC
    ATATGTGAAC TGTTACTAGA GCAATATGAG AAGATTTCAT TCCAGGACCC TCTGAAAAAA
    ATGGTGGCCA AAGCACTTAT GTCTCTCTTA GCCTGTTGCT CCAGTGCTCA GAGTTACGCA
    TGTAAAGCTG GTGTGATTGA CAGCTGTGTG GAGCGCATGA AGCACATTTA CGCAGAGCTT
    CACCTGGAGT CTGTGAAGAC AGGCAGGAAT GCTCACAGGA GGAAGCAGGT GGAGAACAGC
    ATGAGAGAAC TTAAGATGAT TCTAGAGATG TTGCGAAATT GCCTGTATCA ACATAATGAA
    TGCAAAGCTG TGGCTACAGA TTTGCGTCTG GCTGTCGTGC TGTTGGCTTT TTGGCCCTGG
    CTCCTGCTTG ATGATCTCAC CATGGCAGCG GCTCTGGAAC TGCTCTGCGT CTACACAGCG
    AACTGCACAG CGGCTTGCAG TGCTTTATGT TTCAGCAGCA GCAGTAGCAG TGTCAGCGTT
    GGCTCCGTTC AGCCTGTTCT TCCTAAGACT GCGGTCACAA ACTCACTGAT GCATGCAGTG
    ATGAAACTGG CCTCCCAGGT TGCCCCTGAC AACAGCAGCA TCCACAGCCT GGCTTTCTCA
    CTGCTGGCCA ACTTGGCCAT CTCCAGGGAT GGCAAAGGGC TGCTACTGAA GTGTAACTTT
    CTGCAGAACT TCCTGTCTCT CCCCATGCCT AAAGCCAGTG GTCGAGGCGG TGCTCCAGCT
    GTGGAGACAT TGTCTCTCTG GCTGCGGCTG CTGCTGAACG TGTCATTTGG AGAGGACGGA
    CAGCAGATGA TCCTCAGGCT CAGGGGAGCT CTGGAGCTGC TGGTGGAACT GACTCAGTCC
    AAACACTCCA GCGCTAAACC CACCGTCTTG CTCATCCTCC ACAACATCTG CTTCTGCTCC
    GCTAACAAGC CCAAAGTCTT AGCCAACGAT AAAGCTGTTG AACTGCTAGT CTCTTGTTTA
    AGCAGTAGCT CATCAGATAT CCGCAACATA GGAGCTTCTG CACTTTGGGC TTTGTTGCAC
    AACAATCAGA AGGCAAAAGC CACTTTGAAG TGTCCTTCCA TCAGACTGAA AGTTGAAGAG
    GCATTCGTGT CCGCAAGGAA AGATGAAGAG CAGAGCAATG ACAAAGCTGT GAACATCTAT
    CATCTGAAGT GCCTCCGAAA TCTTTCCAAC ATTTTCAACT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49009
    Clone ID Related Accession (Same CDS sequence) XM_017353967.2
    Accession Version XM_017353967.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rotatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGGT GGAGAACAGC
    ATGAGAGAAC TTAAGATGAT TCTAGAGATG TTGCGAAATT GCCTGTATCA ACATAATGAA
    TGCAAAGCTG TGGCTACAGA TTTGCGTCTG GCTGTCGTGC TGTTGGCTTT TTGGCCCTGG
    CTCCTGCTTG ATGATCTCAC CATGGCAGCG GCTCTGGAAC TGCTCTGCGT CTACACAGCG
    AACTGCACAG CGGCTTGCAG TGCTTTATGT TTCAGCAGCA GCAGTAGCAG TGTCAGCGTT
    GGCTCCGTTC AGCCTGTTCT TCCTAAGACT GCGGTCACAA ACTCACTGAT GCATGCAGTG
    ATGAAACTGG CCTCCCAGGT TGCCCCTGAC AACAGCAGCA TCCACAGCCT GGCTTTCTCA
    CTGCTGGCCA ACTTGGCCAT CTCCAGGGAT GGCAAAGGGC TGCTACTGAA GTGTAACTTT
    CTGCAGAACT TCCTGTCTCT CCCCATGCCT AAAGCCAGTG GTCGAGGCGG TGCTCCAGCT
    GTGGAGACAT TGTCTCTCTG GCTGCGGCTG CTGCTGAACG TGTCATTTGG AGAGGACGGA
    CAGCAGATGA TCCTCAGGCT CAGGGGAGCT CTGGAGCTGC TGGTGGAACT GACTCAGTCC
    AAACACTCCA GCGCTAAACC CACCGTCTTG CTCATCCTCC ACAACATCTG CTTCTGCTCC
    GCTAACAAGC CCAAAGTCTT AGCCAACGAT AAAGCTGTTG AACTGCTAGT CTCTTGTTTA
    AGCAGTAGCT CATCAGATAT CCGCAACATA GGAGCTTCTG CACTTTGGGC TTTGTTGCAC
    AACAATCAGA AGGCAAAAGC CACTTTGAAG TGTCCTTCCA TCAGACTGAA AGTTGAAGAG
    GCATTCGTGT CCGCAAGGAA AGATGAAGAG CAGAGCAATG ACAAAGCTGT GAACATCTAT
    CATCTGAAGT GCCTCCGAAA TCTTTCCAAC ATTTTCAACT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209456.2
    CDS91..6795
    Translation

    Target ORF information:

    RefSeq Version XM_017353967.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio rotatin (rttn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353967.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGGT GGAGAACAGC
    ATGAGAGAAC TTAAGATGAT TCTAGAGATG TTGCGAAATT GCCTGTATCA ACATAATGAA
    TGCAAAGCTG TGGCTACAGA TTTGCGTCTG GCTGTCGTGC TGTTGGCTTT TTGGCCCTGG
    CTCCTGCTTG ATGATCTCAC CATGGCAGCG GCTCTGGAAC TGCTCTGCGT CTACACAGCG
    AACTGCACAG CGGCTTGCAG TGCTTTATGT TTCAGCAGCA GCAGTAGCAG TGTCAGCGTT
    GGCTCCGTTC AGCCTGTTCT TCCTAAGACT GCGGTCACAA ACTCACTGAT GCATGCAGTG
    ATGAAACTGG CCTCCCAGGT TGCCCCTGAC AACAGCAGCA TCCACAGCCT GGCTTTCTCA
    CTGCTGGCCA ACTTGGCCAT CTCCAGGGAT GGCAAAGGGC TGCTACTGAA GTGTAACTTT
    CTGCAGAACT TCCTGTCTCT CCCCATGCCT AAAGCCAGTG GTCGAGGCGG TGCTCCAGCT
    GTGGAGACAT TGTCTCTCTG GCTGCGGCTG CTGCTGAACG TGTCATTTGG AGAGGACGGA
    CAGCAGATGA TCCTCAGGCT CAGGGGAGCT CTGGAGCTGC TGGTGGAACT GACTCAGTCC
    AAACACTCCA GCGCTAAACC CACCGTCTTG CTCATCCTCC ACAACATCTG CTTCTGCTCC
    GCTAACAAGC CCAAAGTCTT AGCCAACGAT AAAGCTGTTG AACTGCTAGT CTCTTGTTTA
    AGCAGTAGCT CATCAGATAT CCGCAACATA GGAGCTTCTG CACTTTGGGC TTTGTTGCAC
    AACAATCAGA AGGCAAAAGC CACTTTGAAG TGTCCTTCCA TCAGACTGAA AGTTGAAGAG
    GCATTCGTGT CCGCAAGGAA AGATGAAGAG CAGAGCAATG ACAAAGCTGT GAACATCTAT
    CATCTGAAGT GCCTCCGAAA TCTTTCCAAC ATTTTCAACT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28451
    Clone ID Related Accession (Same CDS sequence) XM_005162659.4 , XM_005162659.3
    Accession Version XM_005162659.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rotatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGCA GGTGGAGAAC
    AGCATGAGAG AACTTAAGAT GATTCTAGAG ATGTTGCGAA ATTGCCTGTA TCAACATAAT
    GAATGCAAAG CTGTGGCTAC AGATTTGCGT CTGGCTGTCG TGCTGTTGGC TTTTTGGCCC
    TGGCTCCTGC TTGATGATCT CACCATGGCA GCGGCTCTGG AACTGCTCTG CGTCTACACA
    GCGAACTGCA CAGCGGCTTG CAGTGCTTTA TGTTTCAGCA GCAGCAGTAG CAGTGTCAGC
    GTTGGCTCCG TTCAGCCTGT TCTTCCTAAG ACTGCGGTCA CAAACTCACT GATGCATGCA
    GTGATGAAAC TGGCCTCCCA GGTTGCCCCT GACAACAGCA GCATCCACAG CCTGGCTTTC
    TCACTGCTGG CCAACTTGGC CATCTCCAGG GATGGCAAAG GGCTGCTACT GAAGTGTAAC
    TTTCTGCAGA ACTTCCTGTC TCTCCCCATG CCTAAAGCCA GTGGTCGAGG CGGTGCTCCA
    GCTGTGGAGA CATTGTCTCT CTGGCTGCGG CTGCTGCTGA ACGTGTCATT TGGAGAGGAC
    GGACAGCAGA TGATCCTCAG GCTCAGGGGA GCTCTGGAGC TGCTGGTGGA ACTGACTCAG
    TCCAAACACT CCAGCGCTAA ACCCACCGTC TTGCTCATCC TCCACAACAT CTGCTTCTGC
    TCCGCTAACA AGCCCAAAGT CTTAGCCAAC GATAAAGCTG TTGAACTGCT AGTCTCTTGT
    TTAAGCAGTA GCTCATCAGA TATCCGCAAC ATAGGAGCTT CTGCACTTTG GGCTTTGTTG
    CACAACAATC AGAAGGCAAA AGCCACTTTG AAGTGTCCTT CCATCAGACT GAAAGTTGAA
    GAGGCATTCG TGTCCGCAAG GAAAGATGAA GAGCAGAGCA ATGACAAAGC TGTGAACATC
    TATCATCTGA AGTGCCTCCG AAATCTTTCC AACATTTTCA ACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162716.1
    CDS91..6798
    Translation

    Target ORF information:

    RefSeq Version XM_005162659.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio rotatin (rttn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162659.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGCA GGTGGAGAAC
    AGCATGAGAG AACTTAAGAT GATTCTAGAG ATGTTGCGAA ATTGCCTGTA TCAACATAAT
    GAATGCAAAG CTGTGGCTAC AGATTTGCGT CTGGCTGTCG TGCTGTTGGC TTTTTGGCCC
    TGGCTCCTGC TTGATGATCT CACCATGGCA GCGGCTCTGG AACTGCTCTG CGTCTACACA
    GCGAACTGCA CAGCGGCTTG CAGTGCTTTA TGTTTCAGCA GCAGCAGTAG CAGTGTCAGC
    GTTGGCTCCG TTCAGCCTGT TCTTCCTAAG ACTGCGGTCA CAAACTCACT GATGCATGCA
    GTGATGAAAC TGGCCTCCCA GGTTGCCCCT GACAACAGCA GCATCCACAG CCTGGCTTTC
    TCACTGCTGG CCAACTTGGC CATCTCCAGG GATGGCAAAG GGCTGCTACT GAAGTGTAAC
    TTTCTGCAGA ACTTCCTGTC TCTCCCCATG CCTAAAGCCA GTGGTCGAGG CGGTGCTCCA
    GCTGTGGAGA CATTGTCTCT CTGGCTGCGG CTGCTGCTGA ACGTGTCATT TGGAGAGGAC
    GGACAGCAGA TGATCCTCAG GCTCAGGGGA GCTCTGGAGC TGCTGGTGGA ACTGACTCAG
    TCCAAACACT CCAGCGCTAA ACCCACCGTC TTGCTCATCC TCCACAACAT CTGCTTCTGC
    TCCGCTAACA AGCCCAAAGT CTTAGCCAAC GATAAAGCTG TTGAACTGCT AGTCTCTTGT
    TTAAGCAGTA GCTCATCAGA TATCCGCAAC ATAGGAGCTT CTGCACTTTG GGCTTTGTTG
    CACAACAATC AGAAGGCAAA AGCCACTTTG AAGTGTCCTT CCATCAGACT GAAAGTTGAA
    GAGGCATTCG TGTCCGCAAG GAAAGATGAA GAGCAGAGCA ATGACAAAGC TGTGAACATC
    TATCATCTGA AGTGCCTCCG AAATCTTTCC AACATTTTCA ACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28451
    Clone ID Related Accession (Same CDS sequence) XM_005162659.4 , XM_005162659.3
    Accession Version XM_005162659.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rotatin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGCA GGTGGAGAAC
    AGCATGAGAG AACTTAAGAT GATTCTAGAG ATGTTGCGAA ATTGCCTGTA TCAACATAAT
    GAATGCAAAG CTGTGGCTAC AGATTTGCGT CTGGCTGTCG TGCTGTTGGC TTTTTGGCCC
    TGGCTCCTGC TTGATGATCT CACCATGGCA GCGGCTCTGG AACTGCTCTG CGTCTACACA
    GCGAACTGCA CAGCGGCTTG CAGTGCTTTA TGTTTCAGCA GCAGCAGTAG CAGTGTCAGC
    GTTGGCTCCG TTCAGCCTGT TCTTCCTAAG ACTGCGGTCA CAAACTCACT GATGCATGCA
    GTGATGAAAC TGGCCTCCCA GGTTGCCCCT GACAACAGCA GCATCCACAG CCTGGCTTTC
    TCACTGCTGG CCAACTTGGC CATCTCCAGG GATGGCAAAG GGCTGCTACT GAAGTGTAAC
    TTTCTGCAGA ACTTCCTGTC TCTCCCCATG CCTAAAGCCA GTGGTCGAGG CGGTGCTCCA
    GCTGTGGAGA CATTGTCTCT CTGGCTGCGG CTGCTGCTGA ACGTGTCATT TGGAGAGGAC
    GGACAGCAGA TGATCCTCAG GCTCAGGGGA GCTCTGGAGC TGCTGGTGGA ACTGACTCAG
    TCCAAACACT CCAGCGCTAA ACCCACCGTC TTGCTCATCC TCCACAACAT CTGCTTCTGC
    TCCGCTAACA AGCCCAAAGT CTTAGCCAAC GATAAAGCTG TTGAACTGCT AGTCTCTTGT
    TTAAGCAGTA GCTCATCAGA TATCCGCAAC ATAGGAGCTT CTGCACTTTG GGCTTTGTTG
    CACAACAATC AGAAGGCAAA AGCCACTTTG AAGTGTCCTT CCATCAGACT GAAAGTTGAA
    GAGGCATTCG TGTCCGCAAG GAAAGATGAA GAGCAGAGCA ATGACAAAGC TGTGAACATC
    TATCATCTGA AGTGCCTCCG AAATCTTTCC AACATTTTCA ACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162716.1
    CDS70..6777
    Translation

    Target ORF information:

    RefSeq Version XM_005162659.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio rotatin (rttn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162659.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGTTAT CGTCACTCAT AAAGAAGATC GGTCACTCCT TAGTGGAAAT CAGGGTCCGG 
    GCTCTTAAAA ATATCAGAAG CAAATTAGAT CATGGCCTCA TCTCTGTTCC TGATCTGGTC
    CAAGAGAAGA TGCTGTTTGT TTTCCTCCTA GAATGGTTTA ATTTTCCGGA GGTTCCAATG
    CAGGAGGAAG TTCTGGAGTT GGTCGACATG CTTTCAAAGC ACCCAACCGG AGCCCAAATG
    CTTCGAGAGG TCGGGGCTGT CGAATTCCTA ACACAACTCT CGCCTAATGT GGATCTTCGT
    CTCCGAACAA TCGTAGACGG CGTCTTCGAT CAGCTCTTCC ACCTCCCAGG GGCCCTTCCA
    TGTTGTCCAC CCACCACCTC CTACGAGCCA CACTTGCAGA CAGAAGAGGA GCCGCCGGCT
    GGGGGATTTT TCCAGTGCAG TAAACCCAAG CAGACAGATG CTCCCACCCC CAGAGTACCA
    TTCAGTACTA GGTGCTTGAA GTTCTCTGTT TTTCCATGGC TGTCTTTGAA CACAACAGAT
    CGACATATAT TGTCATCAAA TGAAAGTTCC CTTAGAAGCG ACAACCACAA CTTAGTGCGA
    ACCACCTGTG AGCTGCTTCG TGATGTCATC ATGCAAGACT TCCCAGCTGA AATATTCCTG
    CAAAGGCCAA GTACAGTTCA AAGTCTGCTG TCACTTCTCG GTCTGAATGT TGATGGTGAT
    GTCAGCTACC TGACGTTCCA GGCATTGGCC TGTCTGCAAC AGCTTTGCAG GAACTTGAAG
    AGCCGCCTAC GATTTTACAG AGATCCAAGC TTCTTCTCAG CCAAACAAGA CCCTGTGTCT
    CAGAACTCAT CCATCTCGTA CCCTCAGGAG GTGAGAGGAA CCCAACGGTC TGGAGTGTCT
    TCTCCAGAAG AAAGCTCCCC CAGACCTTCT GTGGTGGGCA GACCCGGTCA ACGGTTGCGT
    GGTGATGGCC AGGACGGAGA CGCTGCTTCT GCTAGCAGCA GCAGCAGCAG TCATAGAGGG
    GCTCCCCTTA GGCCAGCCCA GCAGTCTCCC CTCCCGTCTG CCCACCCAGA GCTCCCCCCG
    GAGATGATGA TGGAAACGGA GGATGCGCTG GAGCTCCAGC TGCAGCAGTG GAGCCTGGCT
    AGATTCGCCG TAGCAGCCGT AGAGCACGCT CTGCCTCTCA TCAGGACAGA AAGCCTGAAG
    GTTTTCCAGC GGGTGCTAGA GCTGCTGGCG GAGGCGCAGG GGCTGCTGGG AGACAGCGTA
    GCCCCTGAGG TGTGGGACGA GCACAGCCTG ACTGCGACAG AGCTGAGAGA GAAATTACAG
    GCATGTATAG AGATTTTGGG AGATGTTATT TGCTACCACC ATGGCATGAC TTCCTCAGAA
    CATCCAGAAT CGTCTCTGAT CCACCACAGA ATGGTCTTCA CAGGCACTGC CGTTTTCACA
    ATACGTCTCC TGCAAACCTT GTTACCTGTG GAGAAGGCTG CGGATAATCT TCCGGAAAGT
    ACCGTGGCTG CCATTTTCCT GTTGTGTCTT GATATATCCT TCAGCGTGGC GTTCCCCGTC
    ATCCATGAGT CAGCCGTGGC GTATCTAGAG CAGGCTTGCT TTGAAAGATA CAGCCTGTAT
    CGTAGAGTGT CCCGTATTTC TCATTGCATG GAGGCGACAT GCACCTTTTT AAAGGATGCA
    CAGGCTGAGG GCGATAAGAA CTGGCATGAG CTTTTGGAGC TTGCTGACCA AGGAGTAGAT
    GCTTTTCCAT ATCATCAGCA CCTACCGGTG GTGAAGGAAT GTGTCCAGAT TTGCTCCTAC
    CTGTGGAAAT TTGCACAAGC AAGCCCGCTT CTGCAGTCGG AGAGTCAGAA GATATTTCTG
    AAGCTTTTGT CTCATCCTCT GTCCCCAGTG AAGGCAGAAA CCTACTCCTG CACTCTGAGC
    ATTTTGAAGG AGTGTTTGGG AGTTCAGAAC GTGGCCAAAC CTGCCTCATC CATCTGCGCC
    GGAGTGCATT TCTTGCTTCA CTCCCGTGTC CTGTATGAGA TCAGTGTGTT TGGCCTTCAA
    GACCAAGCAA CAGAGGTGAG TGCCGCTGCC AAGGATATTC TGCTTTATCT GCTGAAGGGA
    CGATTGATGA TGACAGCTGC AAGTTGGAGG AGATTTAATG AGGCACTTTA CCCGGTCATT
    CCTATATTAC AGAGCTACGC AGGCACTGAA GACGCCCTGG GAAACTGTAT CCTGCTGCTA
    AGTGAAGCAT CTGCTGAGAA AGGAGATGAG ATGCGTTCCC CGACTGCTAG GCTACGGGCA
    GCCCTCCGGC TTCTGTTCAC CAAACAGCAC ACCGTGAGGA AAATGGCAGT CCAACATCTG
    CTTCCACATC TGACCAGCGC AGAGGGGGCC CAAAACTCCA GACCAACCCT GGACAGCTCC
    ACTCTATCAT GTTTACCAAA TCTCTACAAT CTTAATAAAA GTGTGGACAT CAAGTTCAGC
    AGTTCAGACA AGTCATATGT AAAGGTGGAG TCTGTAAATA AGTTGTACAA TATTCTCGCA
    TCGGAAACGG TAGATCTTGT TTTACGGAAA TCTGCAGCTG AACAGTTGGC TATTGTTCTG
    CAAGATTCAT CAATGCACGC GGTGCTGAAG GCCTTGGGTT TATCAGACAA GCTCACGTCT
    TTCATCACAG AGTGTGTCAA CAGCAATGTG CCGAGCATGG ATTGCTTGTT GGAGTCCAGT
    GCATGCATGC TGAGGAAGCT GGTTTTTGCA GACCCCTCTC TGCGTCACAG TCTGGCTCAA
    CAGACAACTT TTCTCATCAC GCTGCTCAGA GCTTCTCTGA TCATAAAGGA GAACCAGGGT
    GATGTGAGCG AGGCGGCTGC GTTGATGTGC ATTCTGCTGT TTGATGAAAT CGCACGAATG
    GATGTTTGGA CTGACGAAGC TGGCTCAGAC ACAGGCCTGT CTTCCTTCTC ACTTCCTGCA
    GCCATTATAC GGAGGTACAA TTTGCCGTTT CAAGCTCCTT CACATCATGC AGTGAGTCCA
    TACTGTTCAG TTCTTCCTCC ATACTCAGAT CTCCTGAGTC TGAAGCCCGC CTGGGAAGCT
    CTGCAGATCA CCTGGAACAC AGCATGGCAT TCTGGGATTG ATGGATTGCT AGAGCACCTG
    AGGGGTTTTA GAAGTGATGT GCCAGAGTTT CATAGTGATC TGGTGCTGTC TGAATCTCAG
    GCTGTGGTGT TAAGAATCAG CAACATGAGG TCCGGTATAG AGGACTGCAT TCACACGGTT
    TGTGATGCGA GCTCCCACAG CACCGTCACT TTTGCCATCA CTAGAATGCA TCTGTATCTT
    CTTTTTGACT TCCTTGCACT CAAATCCACC ACGCAAAGCT GCAAGAACAT TCTGCAAACC
    CTCAGCTGGC ACAGTGCTGT AGAGAGATTT CTGGTTGTAC GTCCAGCTTG CCATCAAGAT
    GAGAGGCTCC TGGTGGACAT TCTGTCCTTC CTCAACACGT TCTTCAAGCA GAATCAATCT
    GAACTGGACC AGCAGGACCT TAAATGGTTG TTGGAATTAC TCCTAAACCA GGAGTCGCAC
    ACACTGCTGA ACTTGTTGCT GAGCGCTGAT CCGCAGAACC ACACACACGT CCAGTCACAG
    GGGGAAGTGG ACAAACTCCA GGCCCACCTC AGTCACCGGC TACAGAGAGA ACTGACAGGC
    CTCTTCAACA CATTGCTGCT TCTGATGACA TTCACTTCTG ACAGGATGTG TGAGGTTTTG
    TCTGGACCGT TTGCCTCTGA ACTGTCTCTG CGTCTGCTGC AGTGTCTGCG CCTGTCTGAT
    GCTCCGTGCT TTTACGGCCT GCCGTCTCTA GAGCGCACAC TCAGGACCAT GGCCCACGTG
    ACTGCCCTGC CCGGCTGGAG CACCCACACT CCTAGCGTAG AGCCAAACAC GCTCTGTCTC
    AAGTACCTCA GCGGCCTTCT GGAAGTCATA TCTTCATTCT ATGTCGATTT GGGAGGAAAT
    TCAATGTCCT TTATGGGCAA AGGAGTAACA AAAAATGCAG TGATCTGCCT GCTTCATTTG
    TCACACGAGA TGATGAAGGA AAGCACAAAT AAGGACTGGA TATCCTTGTG GTCTCTGGGG
    CGAGAGCAAA GTGCTGATGA GCAAAGTGCC GCTCAGCTTG GCTTGGCCTG GTTAATTCCT
    CTGTGGGTGG ATCGTGACCC TGAGGTCCGC TGTGCCAGTC TGGCAGTGGG TGCGGCTCTG
    AGTTCGCTGG AGAGAGGGTG TGTGGCTCTG TCTGCGAGCT GCCAGAACAT TTCTGGCGGC
    CTGTGGGCCA CAGTACTCAA CATCCTGCTG GAGAAACAGG AGTGCAGTAT GGTGCGCAGA
    GAGGCAGCGT TTATTTTGCA GAACCTCCTT GTGATGCCAA TGCCTGCAAA TGCAGAAGAG
    GCCAAAGAGT GTGCCTGGCA GAGCCCCTGC GTGCATGATG AGGATTCAGG CCTGTGTCTG
    GTCGGGCTTC CTGCCCTGCA GGCTCTGTTG TACCACTGCC AGTTTTTTGA GAGCGTGGGC
    CAGATGGTGA AGAGCTGTTA CACGGGCAGA CATGCCTTCA CCCTCAGCCC CCCTCCGCCG
    CCAGCCAATG TCACCCAGGA TGCAGATTTG AATGATTCTC TGAAGCACTG GAGAGAGATG
    CTTGTTCCTC TAAACCAGAC TCATCCCTCT GGATCTATGT CCACCTCCAG TACTCTGATT
    CCATCAGGAA GCTGCAGTGG GACTCATTCT CCAGTCCCGA CCTCAGGTGT CTGCGCTAAC
    CAGGAAGCTC CAGTTAACAG ACTCACTGCT CAGGGTCAGA GTGACACTGA CAGCTCAGAC
    TCTGCCCTCA CTCAAGACTC ACACCCCGGG GGGCGGAGCG AGCCTGTTGC TATAGTGACC
    CCTCACCTGT TGCTGGCTGT TTGTGGTTTA TTGGACAACC TGCTGGCGGT GCTTCCGGAA
    TTTACTTTCT CTGCCCTCCA ACAGAACCAG ATACTTCAGG GCATGGCCAG TGTGGTGGAT
    GCGGCAGCCA TATCTCAATG TGTCAATGAG CTAAAAACAC CTCTCCTGCC AGCTGCTATG
    GAAGATGCCA GGAACCAGGT GCTGAGTAGC TTGTATTATG TGCAAAGTTT CTGTAAGCTC
    CTGCAGTCAG CAACGATACA CAGTGAGGAT GTGCCGCTCC ACAGAGAGTA CTTCAAACTC
    CTGCTGAACA ACCTCATTTC CATCCTCACG CTGCCTGTCG CTGACTTGGA TGCGCAGACC
    CAGGGTGCAG TGCTCAACAC CTGGTCGGAT GTGTTGACGC TTCTGGCTAC TCTGCTGCGC
    AGGAACCAAG CTGCGGTCGG CCCATCTGTC GCTGTGGCAC TGGGGAAACA CTGGCAGCGT
    TTTTCAGACA CCCTCGGAGT GTGTTTGGAC CAATCGGATG GTGAGTCTTC TCTCTACATA
    TCGACTCTGC ACTTTCTGTG TGTGCTGTTC TCAGACGAGG CTAAGAGGAG AACTGGAGGA
    CACATGGAGG ATCTGAGCAG CTCCTCCATT ACTGCGGTCT TGGCTTCTGA GTCTGGAGAA
    GTCATATGTG AACTGTTACT AGAGCAATAT GAGAAGATTT CATTCCAGGA CCCTCTGAAA
    AAAATGGTGG CCAAAGCACT TATGTCTCTC TTAGCCTGTT GCTCCAGTGC TCAGAGTTAC
    GCATGTAAAG CTGGTGTGAT TGACAGCTGT GTGGAGCGCA TGAAGCACAT TTACGCAGAG
    CTTCACCTGG AGTCTGTGAA GACAGGCAGG AATGCTCACA GGAGGAAGCA GGTGGAGAAC
    AGCATGAGAG AACTTAAGAT GATTCTAGAG ATGTTGCGAA ATTGCCTGTA TCAACATAAT
    GAATGCAAAG CTGTGGCTAC AGATTTGCGT CTGGCTGTCG TGCTGTTGGC TTTTTGGCCC
    TGGCTCCTGC TTGATGATCT CACCATGGCA GCGGCTCTGG AACTGCTCTG CGTCTACACA
    GCGAACTGCA CAGCGGCTTG CAGTGCTTTA TGTTTCAGCA GCAGCAGTAG CAGTGTCAGC
    GTTGGCTCCG TTCAGCCTGT TCTTCCTAAG ACTGCGGTCA CAAACTCACT GATGCATGCA
    GTGATGAAAC TGGCCTCCCA GGTTGCCCCT GACAACAGCA GCATCCACAG CCTGGCTTTC
    TCACTGCTGG CCAACTTGGC CATCTCCAGG GATGGCAAAG GGCTGCTACT GAAGTGTAAC
    TTTCTGCAGA ACTTCCTGTC TCTCCCCATG CCTAAAGCCA GTGGTCGAGG CGGTGCTCCA
    GCTGTGGAGA CATTGTCTCT CTGGCTGCGG CTGCTGCTGA ACGTGTCATT TGGAGAGGAC
    GGACAGCAGA TGATCCTCAG GCTCAGGGGA GCTCTGGAGC TGCTGGTGGA ACTGACTCAG
    TCCAAACACT CCAGCGCTAA ACCCACCGTC TTGCTCATCC TCCACAACAT CTGCTTCTGC
    TCCGCTAACA AGCCCAAAGT CTTAGCCAAC GATAAAGCTG TTGAACTGCT AGTCTCTTGT
    TTAAGCAGTA GCTCATCAGA TATCCGCAAC ATAGGAGCTT CTGCACTTTG GGCTTTGTTG
    CACAACAATC AGAAGGCAAA AGCCACTTTG AAGTGTCCTT CCATCAGACT GAAAGTTGAA
    GAGGCATTCG TGTCCGCAAG GAAAGATGAA GAGCAGAGCA ATGACAAAGC TGTGAACATC
    TATCATCTGA AGTGCCTCCG AAATCTTTCC AACATTTTCA ACTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.